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maximum amplified sequence lengths (excluding primers) are 50 bp and 500 bp, respectively. The maximum amplified sequence lengths (excluding primers) are 50 bp and 500 bp, respectively. The
primers used are TCACAGACCTGTTATTGC and TYTGTCTGSTTRATTSCG (possibility to use primers used are TCACAGACCTGTTATTGC and TYTGTCTGSTTRATTSCG (possibility to use
:doc:`IUPAC codes <../iupac>`). They amplify a short portion of the nuclear 18S gene. The :doc:`IUPAC codes <../iupac>`). They amplify a short portion of the nuclear 18S gene. The
results are saved in the ``mysequence.ecopcr`` file. results are saved in the *mysequence.ecopcr* file.