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@ -158,7 +158,7 @@ Examples
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maximum amplified sequence lengths (excluding primers) are 50 bp and 500 bp, respectively. The
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primers used are TCACAGACCTGTTATTGC and TYTGTCTGSTTRATTSCG (possibility to use
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:doc:`IUPAC codes <../iupac>`). They amplify a short portion of the nuclear 18S gene. The
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results are saved in the ``mysequence.ecopcr`` file.
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results are saved in the *mysequence.ecopcr* file.
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@ -171,11 +171,11 @@ Examples
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Launches an *in silico* PCR on mydatabase (see :doc:`obiconvert <./obiconvert>` for a description
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of the database format), with a maximum of two mismatches for each primer, but with a perfect match
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on the last two nucleotides of the 3' end of each primer (a perfect match can be enforced by adding
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on the last two nucleotides of the 3' end of each primer (a perfect match can be enforced by adding
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a '#' after the considered nucleotide). The minimum and maximum amplified sequence lengths (excluding
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primers) are 80 bp and 120 bp, respectively. The ``-D`` option keeps 50 nucleotides on each side of
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the *in silico* amplified sequences, (including the amplified DNA fragment plus the two target
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sequences of the primers). The primers used are TTAGATACCCCACTATGC and TAGAACAGGCTCCTCTAG. They
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sequences of the primers). The primers used are TTAGATACCCCACTATGC and TAGAACAGGCTCCTCTAG. They
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amplify a short portion of the mitochondrial 12S gene. The ``-r`` option restricts the search to
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vertebrates (7742 is the :doc:`taxid <../attributes/taxid>` of vertebrates). The results are saved
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in the ``mysequence.ecopcr`` file.
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