From 77604f635bc88385112da9d51fb3bb58c94d9265 Mon Sep 17 00:00:00 2001 From: Celine Mercier Date: Wed, 28 Apr 2021 00:52:07 +0200 Subject: [PATCH] Update Wolf tutorial with the OBITools3 --- Wolf-tutorial-with-the-OBITools3.md | 6 +++--- 1 file changed, 3 insertions(+), 3 deletions(-) diff --git a/Wolf-tutorial-with-the-OBITools3.md b/Wolf-tutorial-with-the-OBITools3.md index 356fc2b..82b22a9 100644 --- a/Wolf-tutorial-with-the-OBITools3.md +++ b/Wolf-tutorial-with-the-OBITools3.md @@ -55,11 +55,11 @@ And unzip it: We can check the average alignment score (corresponding approximately to the similarity between the sequences in the overlap): - obi stats -a score_norm wolf/aligned_reads + obi stats -a score_norm wolf/aligned_reads -### 3. Remove unaligned sequence records +### 3. Remove sequence records with a low overlap alignment score: - obi grep -a mode:alignment wolf/aligned_reads wolf/good_sequences + obi grep -p "sequence['score_norm'] > 0.8" wolf/aligned_reads wolf/good_sequences ### 4. Assign each sequence record to the corresponding sample/marker combination