From 4592855095e4db6b62aa826317a66410425e6518 Mon Sep 17 00:00:00 2001 From: Eric Coissac Date: Tue, 17 Jan 2023 19:06:14 +0100 Subject: [PATCH] Adds the new version of the doc as a quarto book --- doc/.DS_Store | Bin 0 -> 6148 bytes doc/.gitignore | 1 + doc/_book/OBITools-V4.pdf | Bin 0 -> 104171 bytes doc/_book/annexes.html | 725 +++---- doc/_book/commands.html | 490 +++++ doc/_book/index.html | 743 +++---- doc/_book/intro.html | 536 ++++++ doc/_book/library.html | 489 +++++ doc/_book/references.html | 329 ++++ doc/_book/search.json | 121 ++ .../site_libs/bootstrap/bootstrap-icons.css | 1704 +++++++++++++++++ .../site_libs/bootstrap/bootstrap-icons.woff | Bin 0 -> 137124 bytes .../site_libs/bootstrap/bootstrap.min.css | 10 + .../site_libs/bootstrap/bootstrap.min.js | 7 + .../site_libs/clipboard/clipboard.min.js | 7 + doc/_book/site_libs/quarto-html/anchor.min.js | 9 + doc/_book/site_libs/quarto-html/popper.min.js | 6 + .../quarto-syntax-highlighting.css | 171 ++ 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2  The OBITools commands

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2.1 Specifying the input files to OBITools commands

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2.2 Options common to most of the OBITools commands

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2.2.1 Specifying input format

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Five sequence formats are accepted for input files. Fasta and Fastq are the main ones, EMBL and Genbank allow the use of flat files produced by these two international databases. The last one, ecoPCR, is maintained for compatibility with previous OBITools and allows to read ecoPCR outputs as sequence files.

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  • --ecopcr : Read data following the ecoPCR output format.
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  • --embl Read data following the EMBL flatfile format.
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  • --genbank Read data following the Genbank flatfile format.
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Several encoding schemes have been proposed for quality scores in Fastq format. Currently, OBITools considers Sanger encoding as the standard. For reasons of compatibility with older datasets produced with Solexa sequencers, it is possible, by using the following option, to force the use of the corresponding quality encoding scheme when reading these older files.

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  • --solexa Decodes quality string according to the Solexa specification. (default: false)
    • +
+
+
+

2.2.2 Specifying output format

+

Only two output sequence formats are supported by OBITools, Fasta and Fastq. Fastq is used when output sequences are associated with quality information. Otherwise, Fasta is the default format. However, it is possible to force the output format by using one of the following two options. Forcing the use of Fasta results in the loss of quality information. Conversely, when the Fastq format is forced with sequences that have no quality data, dummy qualities set to 40 for each nucleotide are added.

+
    +
  • --fasta-output Read data following the ecoPCR output format.
    • +
  • --fastq-output Read data following the EMBL flatfile format.
    • +
+

OBITools allows multiple input files to be specified for a single command.

+
    +
  • --no-order When several input files are provided, indicates that there is no order among them. (default: false)
    • +
+
+
+

2.2.3 Format of the annotations in Fasta and Fastq files

+

OBITools extend the Fasta and Fastq formats by introducing a format for the title lines of these formats allowing to annotate every sequence. While the previous version of OBITools used an ad-hoc format for these annotation, this new version introduce the usage of the standard JSON format to store them.

+

On input, OBITools automatically recognize the format of the annotations, but two options allows to force the parsing following one of them. You should normally not need to use these options.

+
    +

  • --input-OBI-header FASTA/FASTQ title line annotations follow OBI format. (default: false)

    • +

  • --input-json-header FASTA/FASTQ title line annotations follow json format. (default: false)

    • +
+

On output, by default annotation are formatted using the new JSON format. For compatibility with previous version of OBITools and with external scripts and software, it is possible to force the usage of the previous OBITools format.

+
    +

  • --output-OBI-header|-O output FASTA/FASTQ title line annotations follow OBI format. (default: false)

    • +

  • --output-json-header output FASTA/FASTQ title line annotations follow json format. (default: false)

    • +
+ +
+
+
+

2.3 OBITools expression language

+

Several OBITools (e.g. obigrep, obiannotate) allow the user to specify some simple expressions to compute values or define predicates. This expressions are parsed and evaluated using the gval go package, which allows for evaluating go-Like expression.

+
+

2.3.1 Variables usable in the expression

+
+

2.3.1.1 sequence

+

sequence is the sequence object on which the expression is evaluated

+
+
+

2.3.1.2 annotation

+
+
+
+

2.3.2 Function defined in the language

+
+

2.3.2.1 len

+
+
+

2.3.2.2 ismap

+
+
+

2.3.2.3 hasattribute

+
+
+

2.3.2.4 min

+
+
+

2.3.2.5 max

+
+
+
+

2.3.3 Accessing to the sequence annotations

+
+
+
+

2.4 Metabarcode design and quality assessment

+
+

2.4.0.1 obipcr

+
+

Replace the ecoPCR original OBITools

+
+
+
+
+

2.5 File format conversions

+
+

2.5.0.1 obiconvert

+
+
+
+

2.6 Sequence annotations

+
+

2.6.0.1 obitag

+
+
+
+

2.7 Computations on sequences

+
+

2.7.1 obipairing

+
+

Replace the illuminapairedends original OBITools

+
+
+

2.7.1.1 obimultiplex

+
+

Replace the ngsfilter original OBITools

+
+
+
+

2.7.1.2 obicomplement

+
+
+

2.7.1.3 obiclean

+
+
+

2.7.1.4 obiuniq

+
+
+
+
+

2.8 Sequence sampling and filtering

+
+

2.8.0.1 obigrep

+
+
+

2.8.1 Utilities

+
+

2.8.1.1 obicount

+
+
+

2.8.1.2 obidistribute

+
+
+

2.8.1.3 obifind

+
+

Replace the ecofind original OBITools.

+
+ + +
+
+
+ +
+ + +
+ + + + \ No newline at end of file diff --git a/doc/_book/index.html b/doc/_book/index.html index 77ee1ce..57db65f 100644 --- a/doc/_book/index.html +++ b/doc/_book/index.html @@ -1,461 +1,326 @@ - - + - - - The GO OBITools - - + + - - - - - + - - - - - + + - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - + + + + + + + + + + + + + + + + + + + + - + - - -
- -
- +
+
+ +
+ +
+ + + + + +
- +
+
+

OBITools V4

+
+ + + +
+ +
+
Author
+
+

Eric Coissac

+
+
+ +
+
Published
+
+

January 17, 2023

- - - - - - - - - + +
- - - - + + \ No newline at end of file diff --git a/doc/_book/intro.html b/doc/_book/intro.html new file mode 100644 index 0000000..5f78c1e --- /dev/null +++ b/doc/_book/intro.html @@ -0,0 +1,536 @@ + + + + + + + + + +OBITools V4 - 1  The OBITools + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+
+ +
+ +
+ + + + + +
+ +
+
+

1  The OBITools

+
+ + + +
+ + + + +
+ + +
+ +
+

1.1 Aims of OBITools

+
+
+

1.2 File formats usable with OBITools

+
+

1.2.1 The sequence files

+

Sequences can be stored following various format. OBITools knows some of them. The central formats for sequence files manipulated by OBITools scripts are the fasta and fastq format. OBITools extends the both these formats by specifying a syntax to include in the definition line data qualifying the sequence. All file formats use the IUPAC code for encoding nucleotides.

+
+
+

1.2.2 The IUPAC Code

+

The International Union of Pure and Applied Chemistry (IUPAC_) defined the standard code for representing protein or DNA sequences.

+
+

1.2.2.1 Nucleic IUPAC Code

+ + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
CodeNucleotide
AAdenine
CCytosine
GGuanine
TThymine
UUracil
RPurine (A or G)
YPyrimidine (C, T, or U)
MC or A
KT, U, or G
WT, U, or A
SC or G
BC, T, U, or G (not A)
DA, T, U, or G (not C)
HA, T, U, or C (not G)
VA, C, or G (not T, not U)
NAny base (A, C, G, T, or U)
+
+
+
+

1.2.3 The fasta format

+

The fasta format is certainly the most widely used sequence file format. This is certainly due to its great simplicity. It was originally created for the Lipman and Pearson FASTA program. OBITools use in more of the classical :ref:fasta format an :ref:extended version of this format where structured data are included in the title line.

+

In fasta format a sequence is represented by a title line beginning with a > character and the sequences by itself following the :doc:iupac code. The sequence is usually split other severals lines of the same length (expect for the last one)

+
>my_sequence this is my pretty sequence
+ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT
+GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT
+AACGACGTTGCAGTACGTTGCAGT
+

This is no special format for the title line excepting that this line should be unique. Usually the first word following the > character is considered as the sequence identifier. The end of the title line corresponding to a description of the sequence. Several sequences can be concatenated in a same file. The description of the next sequence is just pasted at the end of the record of the previous one

+
>sequence_A this is my first pretty sequence
+ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT
+GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT
+AACGACGTTGCAGTACGTTGCAGT
+>sequence_B this is my second pretty sequence
+ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT
+GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT
+AACGACGTTGCAGTACGTTGCAGT
+>sequence_C this is my third pretty sequence
+ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT
+GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT
+AACGACGTTGCAGTACGTTGCAGT
+
+
+

1.2.4 The fastq sequence format1

+

fastq format is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores. Both the sequence letter and quality score are encoded with a single ASCII character for brevity. It was originally developed at the Wellcome Trust Sanger Institute to bundle a fasta sequence and its quality data, but has recently become the de facto standard for storing the output of high throughput sequencing instruments such as the Illumina Genome Analyzer Illumina (Cock et al. 2010) .

+

A fastq file normally uses four lines per sequence.

+
    +
  • Line 1 begins with a ‘@’ character and is followed by a sequence identifier and an optional description (like a :ref:fasta title line).
    • +
  • Line 2 is the raw sequence letters.
    • +
  • Line 3 begins with a ‘+’ character and is optionally followed by the same sequence identifier (and any description) again.
    • +
  • Line 4 encodes the quality values for the sequence in Line 2, and must contain the same number of symbols as letters in the sequence.
    • +
+

A fastq file containing a single sequence might look like this:

+
@SEQ_ID
+GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTT
++
+!''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65
+

The character ‘!’ represents the lowest quality while ‘~’ is the highest. Here are the quality value characters in left-to-right increasing order of quality (ASCII):

+
!"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~
+

The original Sanger FASTQ files also allowed the sequence and quality strings to be wrapped (split over multiple lines), but this is generally discouraged as it can make parsing complicated due to the unfortunate choice of “@” and “+” as markers (these characters can also occur in the quality string).

+
+

1.2.4.1 Variations

+
+
1.2.4.1.1 Quality
+

A quality value Q is an integer mapping of p (i.e., the probability that the corresponding base call is incorrect). Two different equations have been in use. The first is the standard Sanger variant to assess reliability of a base call, otherwise known as Phred quality score:

+

\[ +Q_\text{sanger} = -10 \, \log_{10} p +\]

+

The Solexa pipeline (i.e., the software delivered with the Illumina Genome Analyzer) earlier used a different mapping, encoding the odds \(\mathbf{p}/(1-\mathbf{p})\) instead of the probability \(\mathbf{p}\):

+

\[ +Q_\text{solexa-prior to v.1.3} = -10 \, \log_{10} \frac{p}{1-p} +\]

+

Although both mappings are asymptotically identical at higher quality values, they differ at lower quality levels (i.e., approximately \(\mathbf{p} > 0.05\), or equivalently, \(\mathbf{Q} < 13\)).

+

|Relationship between Q and p using the Sanger (red) and Solexa (black) equations (described above). The vertical dotted line indicates \(\mathbf{p}= 0.05\), or equivalently, \(Q = 13\).|

+
+
+
+

1.2.4.2 Encoding

+
    +
  • Sanger format can encode a Phred quality score from 0 to 93 using ASCII 33 to 126 (although in raw read data the Phred quality score rarely exceeds 60, higher scores are possible in assemblies or read maps).
    • +
  • Solexa/Illumina 1.0 format can encode a Solexa/Illumina quality score from -5 to 62 using ASCII 59 to 126 (although in raw read data Solexa scores from -5 to 40 only are expected)
    • +
  • Starting with Illumina 1.3 and before Illumina 1.8, the format encoded a Phred quality score from 0 to 62 using ASCII 64 to 126 (although in raw read data Phred scores from 0 to 40 only are expected).
    • +
  • Starting in Illumina 1.5 and before Illumina 1.8, the Phred scores 0 to 2 have a slightly different meaning. The values 0 and 1 are no longer used and the value 2, encoded by ASCII 66 “B”.
    • +
+

Sequencing Control Software, Version 2.6, Catalog # SY-960-2601, Part # 15009921 Rev. A, November 2009] [http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf\\](http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf){.uri} (page 30) states the following: If a read ends with a segment of mostly low quality (Q15 or below), then all of the quality values in the segment are replaced with a value of 2 (encoded as the letter B in Illumina’s text-based encoding of quality scores)… This Q2 indicator does not predict a specific error rate, but rather indicates that a specific final portion of the read should not be used in further analyses. Also, the quality score encoded as “B” letter may occur internally within reads at least as late as pipeline version 1.6, as shown in the following example:

+
@HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1
+TTAATTGGTAAATAAATCTCCTAATAGCTTAGATNTTACCTTNNNNNNNNNNTAGTTTCTTGAGATTTGTTGGGGGAGACATTTTTGTGATTGCCTTGAT
++HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1
+efcfffffcfeefffcffffffddf`feed]`]_Ba_^__[YBBBBBBBBBBRTT\]][]dddd`ddd^dddadd^BBBBBBBBBBBBBBBBBBBBBBBB
+

An alternative interpretation of this ASCII encoding has been proposed. Also, in Illumina runs using PhiX controls, the character ‘B’ was observed to represent an “unknown quality score”. The error rate of ‘B’ reads was roughly 3 phred scores lower the mean observed score of a given run.

+
    +
  • Starting in Illumina 1.8, the quality scores have basically returned to the use of the Sanger format (Phred+33).
    • +
+
+
+
+
+

1.3 File extension

+

There is no standard file extension for a FASTQ file, but .fq and .fastq, are commonly used.

+
+
+

1.4 See also

+
    +
  • :ref:fasta
    • +
+
+
+

1.5 References

+

.. [1] Cock et al (2009) The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants. Nucleic Acids Research,

+

.. [2] Illumina Quality Scores, Tobias Mann, Bioinformatics, San Diego, Illumina 1__

+

.. |Relationship between Q and p using the Sanger (red) and Solexa (black) equations (described above). The vertical dotted line indicates p = 0.05, or equivalently, Q Å 13.| image:: Probability metrics.png

+

See http://en.wikipedia.org/wiki/FASTQ_format

+ + + +
+
+
+
    +

  1. This article uses material from the Wikipedia article FASTQ format which is released under the Creative Commons Attribution-Share-Alike License 3.0↩︎

    2. +
+
+ +
+ + +
+ + + + \ No newline at end of file diff --git a/doc/_book/library.html b/doc/_book/library.html new file mode 100644 index 0000000..8b2c379 --- /dev/null +++ b/doc/_book/library.html @@ -0,0 +1,489 @@ + + + + + + + + + +OBITools V4 - 3  The GO OBITools library + + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+
+ +
+ +
+ + + + + +
+ +
+
+

3  The GO OBITools library

+
+ + + +
+ + + + +
+ + +
+ +
+

3.1 BioSequence

+

The BioSequence class is used to represent biological sequences. It allows for storing : - the sequence itself as a []byte - the sequencing quality score as a []byte if needed - an identifier as a string - a definition as a string - a set of (key, value) pairs in a map[sting]interface{}

+

BioSequence is defined in the obiseq module and is included using the code

+
import (
+    "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
+)
+
+

3.1.1 Creating new instances

+

To create new instance, use

+
    +
  • MakeBioSequence(id string, sequence []byte, definition string) obiseq.BioSequence
    • +
  • NewBioSequence(id string, sequence []byte, definition string) *obiseq.BioSequence
    • +
+

Both create a BioSequence instance, but when the first one returns the instance, the second returns a pointer on the new instance. Two other functions MakeEmptyBioSequence, and NewEmptyBioSequence do the same job but provide an uninitialized objects.

+
    +
  • id parameters corresponds to the unique identifier of the sequence. It mist be a string constituted of a single word (not containing any space).
    • +
  • sequence is the DNA sequence itself, provided as a byte array ([]byte).
    • +
  • definition is a string, potentially empty, but usualy containing a sentence explaining what is that sequence.
    • +
+
import (
+    "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
+)
+
+func main() {
+    myseq := obiseq.NewBiosequence(
+        "seq_GH0001",
+        bytes.FromString("ACGTGTCAGTCG"),
+        "A short test sequence",
+        )
+}
+

When formated as fasta the parameters correspond to the following schema

+
>id definition containing potentially several words
+sequence
+
+
+

3.1.2 End of life of a BioSequence instance

+

When a BioSequence instance is no more used, it is normally taken in charge by the GO garbage collector. You can if you want call the Recycle method on the instance to store the allocated memory element in a pool to limit allocation effort when many sequences are manipulated.

+
+
+

3.1.3 Accessing to the elements of a sequence

+

The different elements of an obiseq.BioSequence must be accessed using a set of methods. For the three main elements provided during the creation of a new instance methodes are :

+
    +
  • Id() string
    • +
  • Sequence() []byte
    • +
  • Definition() string
    • +
+

It exists pending method to change the value of these elements

+
    +
  • SetId(id string)
    • +
  • SetSequence(sequence []byte)
    • +
  • SetDefinition(definition string)
    • +
+
import (
+    "fmt"
+    "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
+)
+
+func main() {
+    myseq := obiseq.NewBiosequence(
+        "seq_GH0001",
+        bytes.FromString("ACGTGTCAGTCG"),
+        "A short test sequence",
+        )
+
+    fmt.Println(myseq.Id())
+    myseq.SetId("SPE01_0001")
+    fmt.Println(myseq.Id())
+}
+
+

3.1.3.1 Different ways for accessing an editing the sequence

+

If Sequence()and SetSequence(sequence []byte) methods are the basic ones, several other methods exist.

+
    +
  • String() string return the sequence directly converted to a string instance.
    • +
  • The Write method family allows for extending an existing sequence following the buffer protocol. +
      +
    • Write(data []byte) (int, error) allows for appending a byte array on 3’ end of the sequence.
      • +
    • WriteString(data string) (int, error) allows for appending a string.
      • +
    • WriteByte(data byte) error allows for appending a single byte.
      • +
    • +
+

The Clear method empties the sequence buffer.

+
import (
+    "fmt"
+    "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
+)
+
+func main() {
+    myseq := obiseq.NewEmptyBiosequence()
+
+    myseq.WriteString("accc")
+    myseq.WriteByte(byte('c'))
+    fmt.Println(myseq.String())
+}
+
+
+

3.1.3.2 Sequence quality scores

+

Sequence quality scores cannot be initialized at the time of instance creation. You must use dedicated methods to add quality scores to a sequence.

+

To be coherent the length of both the DNA sequence and que quality score sequence must be equal. But assessment of this constraint is realized. It is of the programmer responsability to check that invariant.

+

While accessing to the quality scores relies on the method Quality() []byte, setting the quality need to call one of the following method. They run similarly to their sequence dedicated conterpart.

+
    +
  • SetQualities(qualities Quality)
    • +
  • WriteQualities(data []byte) (int, error)
    • +
  • WriteByteQualities(data byte) error
    • +
+

In a way analogous to the Clear method, ClearQualities() empties the sequence of quality scores.

+ + +
+
+
+ +
+ + +
+ + + + \ No newline at end of file diff --git a/doc/_book/references.html b/doc/_book/references.html new file mode 100644 index 0000000..06d6a80 --- /dev/null +++ b/doc/_book/references.html @@ -0,0 +1,329 @@ + + + + + + + + + +OBITools V4 - References + + + + + + + + + + + + + + + + + + + + + + + + + + + +
+
+ +
+ +
+ + + + + +
+ +
+
+

References

+
+ + + +
+ + + + +
+ + +
+ +
+
+Cock, Peter JA, Christopher J Fields, Naohisa Goto, Michael L Heuer, and +Peter M Rice. 2010. “The Sanger FASTQ File Format for Sequences +with Quality Scores, and the Solexa/Illumina FASTQ Variants.” +Nucleic Acids Research 38 (6): 1767–71. +
+
+ + + +
+ + +
+ + + + \ No newline at end of file diff --git a/doc/_book/search.json b/doc/_book/search.json new file mode 100644 index 0000000..4314f8e --- /dev/null +++ b/doc/_book/search.json @@ -0,0 +1,121 @@ +[ + { + "objectID": "index.html", + "href": "index.html", + "title": "OBITools V4", + "section": "", + "text": "Preface\nThe first version of OBITools started to be developed in 2005. This was at the beginning of the DNA metabarcoding story at the Laboratoire d’Ecologie Alpine (LECA) in Grenoble. At that time, with Pierre Taberlet and François Pompanon, we were thinking about the potential of this new methodology under development. PIerre and François developed more the laboratory methods, while I was thinking more about the tools for analysing the sequences produced. Two ideas were behind this development. I wanted something modular, and something easy to extend. To achieve the first goal, I decided to implement obitools as a suite of unix commands mimicking the classic unix commands but dedicated to sequence files. The basic unix commands are very useful for automatically manipulating, parsing and editing text files. They work in flow, line by line on the input text. The result is a new text file that can be used as input for the next command. Such a design makes it possible to quickly develop a text processing pipeline by chaining simple elementary operations. The OBITools are the exact counterpart of these basic Unix commands, but the basic information they process is a sequence (potentially spanning several lines of text), not a single line of text. Most OBITools consume sequence files and produce sequence files. Thus, the principles of chaining and modularity are respected. In order to be able to easily extend the OBITools to keep up with our evolving ideas about processing DNA metabarcoding data, it was decided to develop them using an interpreted language: Python. Python 2, the version available at the time, allowed us to develop the OBITools efficiently. When parts of the algorithms were computationally demanding, they were implemented in C and linked to the Python code. Even though Python is not the most efficient language available, even though computers were not as powerful as they are today, the size of the data we could produce using 454 sequencers or early solexa machines was small enough to be processed in a reasonable time." + }, + { + "objectID": "intro.html#aims-of-obitools", + "href": "intro.html#aims-of-obitools", + "title": "1  The OBITools", + "section": "1.1 Aims of OBITools", + "text": "1.1 Aims of OBITools" + }, + { + "objectID": "intro.html#file-formats-usable-with-obitools", + "href": "intro.html#file-formats-usable-with-obitools", + "title": "1  The OBITools", + "section": "1.2 File formats usable with OBITools", + "text": "1.2 File formats usable with OBITools\n\n1.2.1 The sequence files\nSequences can be stored following various format. OBITools knows some of them. The central formats for sequence files manipulated by OBITools scripts are the fasta and fastq format. OBITools extends the both these formats by specifying a syntax to include in the definition line data qualifying the sequence. All file formats use the IUPAC code for encoding nucleotides.\n\n\n1.2.2 The IUPAC Code\nThe International Union of Pure and Applied Chemistry (IUPAC_) defined the standard code for representing protein or DNA sequences.\n\n1.2.2.1 Nucleic IUPAC Code\n\n\n\nCode\nNucleotide\n\n\n\n\nA\nAdenine\n\n\nC\nCytosine\n\n\nG\nGuanine\n\n\nT\nThymine\n\n\nU\nUracil\n\n\nR\nPurine (A or G)\n\n\nY\nPyrimidine (C, T, or U)\n\n\nM\nC or A\n\n\nK\nT, U, or G\n\n\nW\nT, U, or A\n\n\nS\nC or G\n\n\nB\nC, T, U, or G (not A)\n\n\nD\nA, T, U, or G (not C)\n\n\nH\nA, T, U, or C (not G)\n\n\nV\nA, C, or G (not T, not U)\n\n\nN\nAny base (A, C, G, T, or U)\n\n\n\n\n\n\n1.2.3 The fasta format\nThe fasta format is certainly the most widely used sequence file format. This is certainly due to its great simplicity. It was originally created for the Lipman and Pearson FASTA program. OBITools use in more of the classical :ref:fasta format an :ref:extended version of this format where structured data are included in the title line.\nIn fasta format a sequence is represented by a title line beginning with a > character and the sequences by itself following the :doc:iupac code. The sequence is usually split other severals lines of the same length (expect for the last one)\n>my_sequence this is my pretty sequence\nACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT\nGTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT\nAACGACGTTGCAGTACGTTGCAGT\nThis is no special format for the title line excepting that this line should be unique. Usually the first word following the > character is considered as the sequence identifier. The end of the title line corresponding to a description of the sequence. Several sequences can be concatenated in a same file. The description of the next sequence is just pasted at the end of the record of the previous one\n>sequence_A this is my first pretty sequence\nACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT\nGTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT\nAACGACGTTGCAGTACGTTGCAGT\n>sequence_B this is my second pretty sequence\nACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT\nGTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT\nAACGACGTTGCAGTACGTTGCAGT\n>sequence_C this is my third pretty sequence\nACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT\nGTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT\nAACGACGTTGCAGTACGTTGCAGT\n\n\n1.2.4 The fastq sequence format1\nfastq format is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores. Both the sequence letter and quality score are encoded with a single ASCII character for brevity. It was originally developed at the Wellcome Trust Sanger Institute to bundle a fasta sequence and its quality data, but has recently become the de facto standard for storing the output of high throughput sequencing instruments such as the Illumina Genome Analyzer Illumina (Cock et al. 2010) .\nA fastq file normally uses four lines per sequence.\n\nLine 1 begins with a ‘@’ character and is followed by a sequence identifier and an optional description (like a :ref:fasta title line).\nLine 2 is the raw sequence letters.\nLine 3 begins with a ‘+’ character and is optionally followed by the same sequence identifier (and any description) again.\nLine 4 encodes the quality values for the sequence in Line 2, and must contain the same number of symbols as letters in the sequence.\n\nA fastq file containing a single sequence might look like this:\n@SEQ_ID\nGATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTT\n+\n!''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65\nThe character ‘!’ represents the lowest quality while ‘~’ is the highest. Here are the quality value characters in left-to-right increasing order of quality (ASCII):\n!\"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\\]^_`abcdefghijklmnopqrstuvwxyz{|}~\nThe original Sanger FASTQ files also allowed the sequence and quality strings to be wrapped (split over multiple lines), but this is generally discouraged as it can make parsing complicated due to the unfortunate choice of “@” and “+” as markers (these characters can also occur in the quality string).\n\n1.2.4.1 Variations\n\n1.2.4.1.1 Quality\nA quality value Q is an integer mapping of p (i.e., the probability that the corresponding base call is incorrect). Two different equations have been in use. The first is the standard Sanger variant to assess reliability of a base call, otherwise known as Phred quality score:\n\\[\nQ_\\text{sanger} = -10 \\, \\log_{10} p\n\\]\nThe Solexa pipeline (i.e., the software delivered with the Illumina Genome Analyzer) earlier used a different mapping, encoding the odds \\(\\mathbf{p}/(1-\\mathbf{p})\\) instead of the probability \\(\\mathbf{p}\\):\n\\[\nQ_\\text{solexa-prior to v.1.3} = -10 \\, \\log_{10} \\frac{p}{1-p}\n\\]\nAlthough both mappings are asymptotically identical at higher quality values, they differ at lower quality levels (i.e., approximately \\(\\mathbf{p} > 0.05\\), or equivalently, \\(\\mathbf{Q} < 13\\)).\n|Relationship between Q and p using the Sanger (red) and Solexa (black) equations (described above). The vertical dotted line indicates \\(\\mathbf{p}= 0.05\\), or equivalently, \\(Q = 13\\).|\n\n\n\n1.2.4.2 Encoding\n\nSanger format can encode a Phred quality score from 0 to 93 using ASCII 33 to 126 (although in raw read data the Phred quality score rarely exceeds 60, higher scores are possible in assemblies or read maps).\nSolexa/Illumina 1.0 format can encode a Solexa/Illumina quality score from -5 to 62 using ASCII 59 to 126 (although in raw read data Solexa scores from -5 to 40 only are expected)\nStarting with Illumina 1.3 and before Illumina 1.8, the format encoded a Phred quality score from 0 to 62 using ASCII 64 to 126 (although in raw read data Phred scores from 0 to 40 only are expected).\nStarting in Illumina 1.5 and before Illumina 1.8, the Phred scores 0 to 2 have a slightly different meaning. The values 0 and 1 are no longer used and the value 2, encoded by ASCII 66 “B”.\n\nSequencing Control Software, Version 2.6, Catalog # SY-960-2601, Part # 15009921 Rev. A, November 2009] [http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf\\\\](http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf){.uri} (page 30) states the following: If a read ends with a segment of mostly low quality (Q15 or below), then all of the quality values in the segment are replaced with a value of 2 (encoded as the letter B in Illumina’s text-based encoding of quality scores)… This Q2 indicator does not predict a specific error rate, but rather indicates that a specific final portion of the read should not be used in further analyses. Also, the quality score encoded as “B” letter may occur internally within reads at least as late as pipeline version 1.6, as shown in the following example:\n@HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1\nTTAATTGGTAAATAAATCTCCTAATAGCTTAGATNTTACCTTNNNNNNNNNNTAGTTTCTTGAGATTTGTTGGGGGAGACATTTTTGTGATTGCCTTGAT\n+HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1\nefcfffffcfeefffcffffffddf`feed]`]_Ba_^__[YBBBBBBBBBBRTT\\]][]dddd`ddd^dddadd^BBBBBBBBBBBBBBBBBBBBBBBB\nAn alternative interpretation of this ASCII encoding has been proposed. Also, in Illumina runs using PhiX controls, the character ‘B’ was observed to represent an “unknown quality score”. The error rate of ‘B’ reads was roughly 3 phred scores lower the mean observed score of a given run.\n\nStarting in Illumina 1.8, the quality scores have basically returned to the use of the Sanger format (Phred+33)." + }, + { + "objectID": "intro.html#file-extension", + "href": "intro.html#file-extension", + "title": "1  The OBITools", + "section": "1.3 File extension", + "text": "1.3 File extension\nThere is no standard file extension for a FASTQ file, but .fq and .fastq, are commonly used." + }, + { + "objectID": "intro.html#see-also", + "href": "intro.html#see-also", + "title": "1  The OBITools", + "section": "1.4 See also", + "text": "1.4 See also\n\n:ref:fasta" + }, + { + "objectID": "intro.html#references", + "href": "intro.html#references", + "title": "1  The OBITools", + "section": "1.5 References", + "text": "1.5 References\n.. [1] Cock et al (2009) The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants. Nucleic Acids Research,\n.. [2] Illumina Quality Scores, Tobias Mann, Bioinformatics, San Diego, Illumina 1__\n.. |Relationship between Q and p using the Sanger (red) and Solexa (black) equations (described above). The vertical dotted line indicates p = 0.05, or equivalently, Q Å 13.| image:: Probability metrics.png\nSee http://en.wikipedia.org/wiki/FASTQ_format\n\n\n\n\nCock, Peter JA, Christopher J Fields, Naohisa Goto, Michael L Heuer, and Peter M Rice. 2010. “The Sanger FASTQ File Format for Sequences with Quality Scores, and the Solexa/Illumina FASTQ Variants.” Nucleic Acids Research 38 (6): 1767–71." + }, + { + "objectID": "commands.html#specifying-the-input-files-to-obitools-commands", + "href": "commands.html#specifying-the-input-files-to-obitools-commands", + "title": "2  The OBITools commands", + "section": "2.1 Specifying the input files to OBITools commands", + "text": "2.1 Specifying the input files to OBITools commands" + }, + { + "objectID": "commands.html#options-common-to-most-of-the-obitools-commands", + "href": "commands.html#options-common-to-most-of-the-obitools-commands", + "title": "2  The OBITools commands", + "section": "2.2 Options common to most of the OBITools commands", + "text": "2.2 Options common to most of the OBITools commands\n\n2.2.1 Specifying input format\nFive sequence formats are accepted for input files. Fasta and Fastq are the main ones, EMBL and Genbank allow the use of flat files produced by these two international databases. The last one, ecoPCR, is maintained for compatibility with previous OBITools and allows to read ecoPCR outputs as sequence files.\n\n--ecopcr : Read data following the ecoPCR output format.\n--embl Read data following the EMBL flatfile format.\n--genbank Read data following the Genbank flatfile format.\n\nSeveral encoding schemes have been proposed for quality scores in Fastq format. Currently, OBITools considers Sanger encoding as the standard. For reasons of compatibility with older datasets produced with Solexa sequencers, it is possible, by using the following option, to force the use of the corresponding quality encoding scheme when reading these older files.\n\n--solexa Decodes quality string according to the Solexa specification. (default: false)\n\n\n\n2.2.2 Specifying output format\nOnly two output sequence formats are supported by OBITools, Fasta and Fastq. Fastq is used when output sequences are associated with quality information. Otherwise, Fasta is the default format. However, it is possible to force the output format by using one of the following two options. Forcing the use of Fasta results in the loss of quality information. Conversely, when the Fastq format is forced with sequences that have no quality data, dummy qualities set to 40 for each nucleotide are added.\n\n--fasta-output Read data following the ecoPCR output format.\n--fastq-output Read data following the EMBL flatfile format.\n\nOBITools allows multiple input files to be specified for a single command.\n\n--no-order When several input files are provided, indicates that there is no order among them. (default: false)\n\n\n\n2.2.3 Format of the annotations in Fasta and Fastq files\nOBITools extend the Fasta and Fastq formats by introducing a format for the title lines of these formats allowing to annotate every sequence. While the previous version of OBITools used an ad-hoc format for these annotation, this new version introduce the usage of the standard JSON format to store them.\nOn input, OBITools automatically recognize the format of the annotations, but two options allows to force the parsing following one of them. You should normally not need to use these options.\n\n--input-OBI-header FASTA/FASTQ title line annotations follow OBI format. (default: false)\n--input-json-header FASTA/FASTQ title line annotations follow json format. (default: false)\n\nOn output, by default annotation are formatted using the new JSON format. For compatibility with previous version of OBITools and with external scripts and software, it is possible to force the usage of the previous OBITools format.\n\n--output-OBI-header|-O output FASTA/FASTQ title line annotations follow OBI format. (default: false)\n--output-json-header output FASTA/FASTQ title line annotations follow json format. (default: false)\n\n\n2.2.3.1 System related options\n\n--debug (default: false)\n--help\\|-h\\|-? (default: false)\n--max-cpu Number of parallele threads computing the result (default: 10)\n--workers\\|-w Number of parallele threads computing the result (default: 9)" + }, + { + "objectID": "commands.html#obitools-expression-language", + "href": "commands.html#obitools-expression-language", + "title": "2  The OBITools commands", + "section": "2.3 OBITools expression language", + "text": "2.3 OBITools expression language\nSeveral OBITools (e.g. obigrep, obiannotate) allow the user to specify some simple expressions to compute values or define predicates. This expressions are parsed and evaluated using the gval go package, which allows for evaluating go-Like expression.\n\n2.3.1 Variables usable in the expression\n\n2.3.1.1 sequence\nsequence is the sequence object on which the expression is evaluated\n\n\n2.3.1.2 annotation\n\n\n\n2.3.2 Function defined in the language\n\n2.3.2.1 len\n\n\n2.3.2.2 ismap\n\n\n2.3.2.3 hasattribute\n\n\n2.3.2.4 min\n\n\n2.3.2.5 max\n\n\n\n2.3.3 Accessing to the sequence annotations" + }, + { + "objectID": "commands.html#metabarcode-design-and-quality-assessment", + "href": "commands.html#metabarcode-design-and-quality-assessment", + "title": "2  The OBITools commands", + "section": "2.4 Metabarcode design and quality assessment", + "text": "2.4 Metabarcode design and quality assessment\n\n2.4.0.1 obipcr\n\nReplace the ecoPCR original OBITools" + }, + { + "objectID": "commands.html#file-format-conversions", + "href": "commands.html#file-format-conversions", + "title": "2  The OBITools commands", + "section": "2.5 File format conversions", + "text": "2.5 File format conversions\n\n2.5.0.1 obiconvert" + }, + { + "objectID": "commands.html#sequence-annotations", + "href": "commands.html#sequence-annotations", + "title": "2  The OBITools commands", + "section": "2.6 Sequence annotations", + "text": "2.6 Sequence annotations\n\n2.6.0.1 obitag" + }, + { + "objectID": "commands.html#computations-on-sequences", + "href": "commands.html#computations-on-sequences", + "title": "2  The OBITools commands", + "section": "2.7 Computations on sequences", + "text": "2.7 Computations on sequences\n\n2.7.1 obipairing\n\nReplace the illuminapairedends original OBITools\n\n\n2.7.1.1 obimultiplex\n\nReplace the ngsfilter original OBITools\n\n\n\n2.7.1.2 obicomplement\n\n\n2.7.1.3 obiclean\n\n\n2.7.1.4 obiuniq" + }, + { + "objectID": "commands.html#sequence-sampling-and-filtering", + "href": "commands.html#sequence-sampling-and-filtering", + "title": "2  The OBITools commands", + "section": "2.8 Sequence sampling and filtering", + "text": "2.8 Sequence sampling and filtering\n\n2.8.0.1 obigrep\n\n\n2.8.1 Utilities\n\n2.8.1.1 obicount\n\n\n2.8.1.2 obidistribute\n\n\n2.8.1.3 obifind\n\nReplace the ecofind original OBITools." + }, + { + "objectID": "library.html#biosequence", + "href": "library.html#biosequence", + "title": "3  The GO OBITools library", + "section": "3.1 BioSequence", + "text": "3.1 BioSequence\nThe BioSequence class is used to represent biological sequences. It allows for storing : - the sequence itself as a []byte - the sequencing quality score as a []byte if needed - an identifier as a string - a definition as a string - a set of (key, value) pairs in a map[sting]interface{}\nBioSequence is defined in the obiseq module and is included using the code\nimport (\n \"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq\"\n)\n\n3.1.1 Creating new instances\nTo create new instance, use\n\nMakeBioSequence(id string, sequence []byte, definition string) obiseq.BioSequence\nNewBioSequence(id string, sequence []byte, definition string) *obiseq.BioSequence\n\nBoth create a BioSequence instance, but when the first one returns the instance, the second returns a pointer on the new instance. Two other functions MakeEmptyBioSequence, and NewEmptyBioSequence do the same job but provide an uninitialized objects.\n\nid parameters corresponds to the unique identifier of the sequence. It mist be a string constituted of a single word (not containing any space).\nsequence is the DNA sequence itself, provided as a byte array ([]byte).\ndefinition is a string, potentially empty, but usualy containing a sentence explaining what is that sequence.\n\nimport (\n \"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq\"\n)\n\nfunc main() {\n myseq := obiseq.NewBiosequence(\n \"seq_GH0001\",\n bytes.FromString(\"ACGTGTCAGTCG\"),\n \"A short test sequence\",\n )\n}\nWhen formated as fasta the parameters correspond to the following schema\n>id definition containing potentially several words\nsequence\n\n\n3.1.2 End of life of a BioSequence instance\nWhen a BioSequence instance is no more used, it is normally taken in charge by the GO garbage collector. You can if you want call the Recycle method on the instance to store the allocated memory element in a pool to limit allocation effort when many sequences are manipulated.\n\n\n3.1.3 Accessing to the elements of a sequence\nThe different elements of an obiseq.BioSequence must be accessed using a set of methods. For the three main elements provided during the creation of a new instance methodes are :\n\nId() string\nSequence() []byte\nDefinition() string\n\nIt exists pending method to change the value of these elements\n\nSetId(id string)\nSetSequence(sequence []byte)\nSetDefinition(definition string)\n\nimport (\n \"fmt\"\n \"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq\"\n)\n\nfunc main() {\n myseq := obiseq.NewBiosequence(\n \"seq_GH0001\",\n bytes.FromString(\"ACGTGTCAGTCG\"),\n \"A short test sequence\",\n )\n\n fmt.Println(myseq.Id())\n myseq.SetId(\"SPE01_0001\")\n fmt.Println(myseq.Id())\n}\n\n3.1.3.1 Different ways for accessing an editing the sequence\nIf Sequence()and SetSequence(sequence []byte) methods are the basic ones, several other methods exist.\n\nString() string return the sequence directly converted to a string instance.\nThe Write method family allows for extending an existing sequence following the buffer protocol.\n\nWrite(data []byte) (int, error) allows for appending a byte array on 3’ end of the sequence.\nWriteString(data string) (int, error) allows for appending a string.\nWriteByte(data byte) error allows for appending a single byte.\n\n\nThe Clear method empties the sequence buffer.\nimport (\n \"fmt\"\n \"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq\"\n)\n\nfunc main() {\n myseq := obiseq.NewEmptyBiosequence()\n\n myseq.WriteString(\"accc\")\n myseq.WriteByte(byte('c'))\n fmt.Println(myseq.String())\n}\n\n\n3.1.3.2 Sequence quality scores\nSequence quality scores cannot be initialized at the time of instance creation. You must use dedicated methods to add quality scores to a sequence.\nTo be coherent the length of both the DNA sequence and que quality score sequence must be equal. But assessment of this constraint is realized. It is of the programmer responsability to check that invariant.\nWhile accessing to the quality scores relies on the method Quality() []byte, setting the quality need to call one of the following method. They run similarly to their sequence dedicated conterpart.\n\nSetQualities(qualities Quality)\nWriteQualities(data []byte) (int, error)\nWriteByteQualities(data byte) error\n\nIn a way analogous to the Clear method, ClearQualities() empties the sequence of quality scores." + }, + { + "objectID": "annexes.html", + "href": "annexes.html", + "title": "4  Annexes", + "section": "", + "text": "4.0.1 Sequence attributes\n\n4.0.1.1 Reserved sequence attributes\n\n4.0.1.1.1 ali_dir\n\n4.0.1.1.1.1 Type : string\nThe attribute can contain 2 string values \"left\" or \"right\".\n\n\n4.0.1.1.1.2 Set by the obipairing tool\nThe alignment generated by obipairing is a 3’-end gap free algorithm. Two cases can occur when aligning the forward and reverse reads. If the barcode is long enough, both the reads overlap only on their 3’ ends. In such case, the alignment direction ali_dir is set to left. If the barcode is shorter than the read length, the paired reads overlap by their 5’ ends, and the complete barcode is sequenced by both the reads. In that later case, ali_dir is set to right.\n\n\n\n4.0.1.1.2 ali_length\n\n4.0.1.1.2.1 Set by the obipairing tool\nLength of the aligned parts when merging forward and reverse reads\n\n\n\n4.0.1.1.3 count : the number of sequence occurrences\n\n4.0.1.1.3.1 Set by the obiuniq tool\nThe count attribute indicates how-many strictly identical sequences have been merged in a single record. It contains an integer value. If it is absent this means that the sequence record represents a single occurrence of the sequence.\n\n\n4.0.1.1.3.2 Getter : method Count()\nThe Count() method allows to access to the count attribute as an integer value. If the count attribute is not defined for the given sequence, the value 1 is returned\n\n\n\n4.0.1.1.4 merged_*\n\n4.0.1.1.4.1 Type : map[string]int\n\n\n4.0.1.1.4.2 Set by the obiuniq tool\nThe -m option of the obiuniq tools allows for keeping track of the distribution of the values stored in given attribute of interest. Often this option is used to summarise distribution of a sequence variant accross samples when obiuniq is run after running obimultiplex. The actual name of the attribute depends on the name of the monitored attribute. If -m option is used with the attribute sample, then this attribute names merged_sample.\n\n\n\n4.0.1.1.5 mode\n\n4.0.1.1.5.1 Set by the obipairing tool\nobitag_ref_index\n\n\n4.0.1.1.5.2 Set by the obirefidx tool.\nIt resumes to which taxonomic annotation a match to that sequence must lead according to the number of differences existing between the query sequence and the reference sequence having that tag.\n\n\n4.0.1.1.5.3 Getter : method Count()\n\n\n\n4.0.1.1.6 pairing_mismatches\n\n4.0.1.1.6.1 Set by the obipairing tool\n\n\n\n4.0.1.1.7 score\n\n4.0.1.1.7.1 Set by the obipairing tool\n\n\n\n4.0.1.1.8 score_norm\n\n4.0.1.1.8.1 Set by the obipairing tool" + }, + { + "objectID": "references.html", + "href": "references.html", + "title": "References", + "section": "", + "text": "Cock, Peter JA, Christopher J Fields, Naohisa Goto, Michael L Heuer, and\nPeter M Rice. 2010. “The Sanger FASTQ File Format for Sequences\nwith Quality Scores, and the Solexa/Illumina FASTQ Variants.”\nNucleic 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n=this._getDimension();this._element.classList.remove(ct),this._element.classList.add(ht),this._element.style[n]=0,this._addAriaAndCollapsedClass(this._triggerArray,!0),this._isTransitioning=!0;const s=`scroll${n[0].toUpperCase()+n.slice(1)}`;this._queueCallback((()=>{this._isTransitioning=!1,this._element.classList.remove(ht),this._element.classList.add(ct,lt),this._element.style[n]="",j.trigger(this._element,"shown.bs.collapse")}),this._element,!0),this._element.style[n]=`${this._element[s]}px`}hide(){if(this._isTransitioning||!this._isShown())return;if(j.trigger(this._element,"hide.bs.collapse").defaultPrevented)return;const t=this._getDimension();this._element.style[t]=`${this._element.getBoundingClientRect()[t]}px`,u(this._element),this._element.classList.add(ht),this._element.classList.remove(ct,lt);const e=this._triggerArray.length;for(let t=0;t{this._isTransitioning=!1,this._element.classList.remove(ht),this._element.classList.add(ct),j.trigger(this._element,"hidden.bs.collapse")}),this._element,!0)}_isShown(t=this._element){return t.classList.contains(lt)}_getConfig(t){return(t={...rt,...U.getDataAttributes(this._element),...t}).toggle=Boolean(t.toggle),t.parent=r(t.parent),a(ot,t,at),t}_getDimension(){return this._element.classList.contains("collapse-horizontal")?"width":"height"}_initializeChildren(){if(!this._config.parent)return;const t=V.find(ut,this._config.parent);V.find(ft,this._config.parent).filter((e=>!t.includes(e))).forEach((t=>{const e=n(t);e&&this._addAriaAndCollapsedClass([t],this._isShown(e))}))}_addAriaAndCollapsedClass(t,e){t.length&&t.forEach((t=>{e?t.classList.remove(dt):t.classList.add(dt),t.setAttribute("aria-expanded",e)}))}static jQueryInterface(t){return this.each((function(){const e={};"string"==typeof t&&/show|hide/.test(t)&&(e.toggle=!1);const 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0===p?0:p,g=ke(e,{boundary:l,rootBoundary:c,padding:d,altBoundary:h}),_=Ut(e.placement),b=ce(e.placement),v=!b,y=ee(_),w="x"===y?"y":"x",E=e.modifiersData.popperOffsets,A=e.rects.reference,T=e.rects.popper,O="function"==typeof m?m(Object.assign({},e.rects,{placement:e.placement})):m,C={x:0,y:0};if(E){if(o||a){var k="y"===y?mt:bt,L="y"===y?gt:_t,x="y"===y?"height":"width",D=E[y],S=E[y]+g[k],N=E[y]-g[L],I=f?-T[x]/2:0,P=b===wt?A[x]:T[x],j=b===wt?-T[x]:-A[x],M=e.elements.arrow,H=f&&M?Kt(M):{width:0,height:0},B=e.modifiersData["arrow#persistent"]?e.modifiersData["arrow#persistent"].padding:{top:0,right:0,bottom:0,left:0},R=B[k],W=B[L],$=oe(0,A[x],H[x]),z=v?A[x]/2-I-$-R-O:P-$-R-O,q=v?-A[x]/2+I+$+W+O:j+$+W+O,F=e.elements.arrow&&te(e.elements.arrow),U=F?"y"===y?F.clientTop||0:F.clientLeft||0:0,V=e.modifiersData.offset?e.modifiersData.offset[e.placement][y]:0,K=E[y]+z-V-U,X=E[y]+q-V;if(o){var Y=oe(f?ne(S,K):S,D,f?ie(N,X):N);E[y]=Y,C[y]=Y-D}if(a){var Q="x"===y?mt:bt,G="x"===y?gt:_t,Z=E[w],J=Z+g[Q],tt=Z-g[G],et=oe(f?ne(J,K):J,Z,f?ie(tt,X):tt);E[w]=et,C[w]=et-Z}}e.modifiersData[n]=C}},requiresIfExists:["offset"]};function Me(t,e,i){void 0===i&&(i=!1);var n=zt(e);zt(e)&&function(t){var e=t.getBoundingClientRect();e.width,t.offsetWidth,e.height,t.offsetHeight}(e);var s,o,r=Gt(e),a=Vt(t),l={scrollLeft:0,scrollTop:0},c={x:0,y:0};return(n||!n&&!i)&&(("body"!==Rt(e)||we(r))&&(l=(s=e)!==Wt(s)&&zt(s)?{scrollLeft:(o=s).scrollLeft,scrollTop:o.scrollTop}:ve(s)),zt(e)?((c=Vt(e)).x+=e.clientLeft,c.y+=e.clientTop):r&&(c.x=ye(r))),{x:a.left+l.scrollLeft-c.x,y:a.top+l.scrollTop-c.y,width:a.width,height:a.height}}function He(t){var e=new Map,i=new Set,n=[];function s(t){i.add(t.name),[].concat(t.requires||[],t.requiresIfExists||[]).forEach((function(t){if(!i.has(t)){var n=e.get(t);n&&s(n)}})),n.push(t)}return t.forEach((function(t){e.set(t.name,t)})),t.forEach((function(t){i.has(t.name)||s(t)})),n}var Be={placement:"bottom",modifiers:[],strategy:"absolute"};function Re(){for(var t=arguments.length,e=new Array(t),i=0;ij.on(t,"mouseover",d))),this._element.focus(),this._element.setAttribute("aria-expanded",!0),this._menu.classList.add(Je),this._element.classList.add(Je),j.trigger(this._element,"shown.bs.dropdown",t)}hide(){if(c(this._element)||!this._isShown(this._menu))return;const t={relatedTarget:this._element};this._completeHide(t)}dispose(){this._popper&&this._popper.destroy(),super.dispose()}update(){this._inNavbar=this._detectNavbar(),this._popper&&this._popper.update()}_completeHide(t){j.trigger(this._element,"hide.bs.dropdown",t).defaultPrevented||("ontouchstart"in document.documentElement&&[].concat(...document.body.children).forEach((t=>j.off(t,"mouseover",d))),this._popper&&this._popper.destroy(),this._menu.classList.remove(Je),this._element.classList.remove(Je),this._element.setAttribute("aria-expanded","false"),U.removeDataAttribute(this._menu,"popper"),j.trigger(this._element,"hidden.bs.dropdown",t))}_getConfig(t){if(t={...this.constructor.Default,...U.getDataAttributes(this._element),...t},a(Ue,t,this.constructor.DefaultType),"object"==typeof t.reference&&!o(t.reference)&&"function"!=typeof t.reference.getBoundingClientRect)throw new TypeError(`${Ue.toUpperCase()}: Option "reference" provided type "object" without a required "getBoundingClientRect" method.`);return t}_createPopper(t){if(void 0===Fe)throw new TypeError("Bootstrap's dropdowns require Popper (https://popper.js.org)");let e=this._element;"parent"===this._config.reference?e=t:o(this._config.reference)?e=r(this._config.reference):"object"==typeof this._config.reference&&(e=this._config.reference);const i=this._getPopperConfig(),n=i.modifiers.find((t=>"applyStyles"===t.name&&!1===t.enabled));this._popper=qe(e,this._menu,i),n&&U.setDataAttribute(this._menu,"popper","static")}_isShown(t=this._element){return t.classList.contains(Je)}_getMenuElement(){return V.next(this._element,ei)[0]}_getPlacement(){const t=this._element.parentNode;if(t.classList.contains("dropend"))return ri;if(t.classList.contains("dropstart"))return ai;const e="end"===getComputedStyle(this._menu).getPropertyValue("--bs-position").trim();return t.classList.contains("dropup")?e?ni:ii:e?oi:si}_detectNavbar(){return null!==this._element.closest(".navbar")}_getOffset(){const{offset:t}=this._config;return"string"==typeof t?t.split(",").map((t=>Number.parseInt(t,10))):"function"==typeof t?e=>t(e,this._element):t}_getPopperConfig(){const t={placement:this._getPlacement(),modifiers:[{name:"preventOverflow",options:{boundary:this._config.boundary}},{name:"offset",options:{offset:this._getOffset()}}]};return"static"===this._config.display&&(t.modifiers=[{name:"applyStyles",enabled:!1}]),{...t,..."function"==typeof this._config.popperConfig?this._config.popperConfig(t):this._config.popperConfig}}_selectMenuItem({key:t,target:e}){const i=V.find(".dropdown-menu .dropdown-item:not(.disabled):not(:disabled)",this._menu).filter(l);i.length&&v(i,e,t===Ye,!i.includes(e)).focus()}static jQueryInterface(t){return this.each((function(){const e=hi.getOrCreateInstance(this,t);if("string"==typeof t){if(void 0===e[t])throw new TypeError(`No method named "${t}"`);e[t]()}}))}static clearMenus(t){if(t&&(2===t.button||"keyup"===t.type&&"Tab"!==t.key))return;const e=V.find(ti);for(let i=0,n=e.length;ie+t)),this._setElementAttributes(di,"paddingRight",(e=>e+t)),this._setElementAttributes(ui,"marginRight",(e=>e-t))}_disableOverFlow(){this._saveInitialAttribute(this._element,"overflow"),this._element.style.overflow="hidden"}_setElementAttributes(t,e,i){const n=this.getWidth();this._applyManipulationCallback(t,(t=>{if(t!==this._element&&window.innerWidth>t.clientWidth+n)return;this._saveInitialAttribute(t,e);const s=window.getComputedStyle(t)[e];t.style[e]=`${i(Number.parseFloat(s))}px`}))}reset(){this._resetElementAttributes(this._element,"overflow"),this._resetElementAttributes(this._element,"paddingRight"),this._resetElementAttributes(di,"paddingRight"),this._resetElementAttributes(ui,"marginRight")}_saveInitialAttribute(t,e){const i=t.style[e];i&&U.setDataAttribute(t,e,i)}_resetElementAttributes(t,e){this._applyManipulationCallback(t,(t=>{const i=U.getDataAttribute(t,e);void 0===i?t.style.removeProperty(e):(U.removeDataAttribute(t,e),t.style[e]=i)}))}_applyManipulationCallback(t,e){o(t)?e(t):V.find(t,this._element).forEach(e)}isOverflowing(){return this.getWidth()>0}}const pi={className:"modal-backdrop",isVisible:!0,isAnimated:!1,rootElement:"body",clickCallback:null},mi={className:"string",isVisible:"boolean",isAnimated:"boolean",rootElement:"(element|string)",clickCallback:"(function|null)"},gi="show",_i="mousedown.bs.backdrop";class bi{constructor(t){this._config=this._getConfig(t),this._isAppended=!1,this._element=null}show(t){this._config.isVisible?(this._append(),this._config.isAnimated&&u(this._getElement()),this._getElement().classList.add(gi),this._emulateAnimation((()=>{_(t)}))):_(t)}hide(t){this._config.isVisible?(this._getElement().classList.remove(gi),this._emulateAnimation((()=>{this.dispose(),_(t)}))):_(t)}_getElement(){if(!this._element){const t=document.createElement("div");t.className=this._config.className,this._config.isAnimated&&t.classList.add("fade"),this._element=t}return this._element}_getConfig(t){return(t={...pi,..."object"==typeof t?t:{}}).rootElement=r(t.rootElement),a("backdrop",t,mi),t}_append(){this._isAppended||(this._config.rootElement.append(this._getElement()),j.on(this._getElement(),_i,(()=>{_(this._config.clickCallback)})),this._isAppended=!0)}dispose(){this._isAppended&&(j.off(this._element,_i),this._element.remove(),this._isAppended=!1)}_emulateAnimation(t){b(t,this._getElement(),this._config.isAnimated)}}const vi={trapElement:null,autofocus:!0},yi={trapElement:"element",autofocus:"boolean"},wi=".bs.focustrap",Ei="backward";class Ai{constructor(t){this._config=this._getConfig(t),this._isActive=!1,this._lastTabNavDirection=null}activate(){const{trapElement:t,autofocus:e}=this._config;this._isActive||(e&&t.focus(),j.off(document,wi),j.on(document,"focusin.bs.focustrap",(t=>this._handleFocusin(t))),j.on(document,"keydown.tab.bs.focustrap",(t=>this._handleKeydown(t))),this._isActive=!0)}deactivate(){this._isActive&&(this._isActive=!1,j.off(document,wi))}_handleFocusin(t){const{target:e}=t,{trapElement:i}=this._config;if(e===document||e===i||i.contains(e))return;const n=V.focusableChildren(i);0===n.length?i.focus():this._lastTabNavDirection===Ei?n[n.length-1].focus():n[0].focus()}_handleKeydown(t){"Tab"===t.key&&(this._lastTabNavDirection=t.shiftKey?Ei:"forward")}_getConfig(t){return t={...vi,..."object"==typeof t?t:{}},a("focustrap",t,yi),t}}const Ti="modal",Oi="Escape",Ci={backdrop:!0,keyboard:!0,focus:!0},ki={backdrop:"(boolean|string)",keyboard:"boolean",focus:"boolean"},Li="hidden.bs.modal",xi="show.bs.modal",Di="resize.bs.modal",Si="click.dismiss.bs.modal",Ni="keydown.dismiss.bs.modal",Ii="mousedown.dismiss.bs.modal",Pi="modal-open",ji="show",Mi="modal-static";class Hi extends B{constructor(t,e){super(t),this._config=this._getConfig(e),this._dialog=V.findOne(".modal-dialog",this._element),this._backdrop=this._initializeBackDrop(),this._focustrap=this._initializeFocusTrap(),this._isShown=!1,this._ignoreBackdropClick=!1,this._isTransitioning=!1,this._scrollBar=new fi}static get Default(){return Ci}static get NAME(){return Ti}toggle(t){return this._isShown?this.hide():this.show(t)}show(t){this._isShown||this._isTransitioning||j.trigger(this._element,xi,{relatedTarget:t}).defaultPrevented||(this._isShown=!0,this._isAnimated()&&(this._isTransitioning=!0),this._scrollBar.hide(),document.body.classList.add(Pi),this._adjustDialog(),this._setEscapeEvent(),this._setResizeEvent(),j.on(this._dialog,Ii,(()=>{j.one(this._element,"mouseup.dismiss.bs.modal",(t=>{t.target===this._element&&(this._ignoreBackdropClick=!0)}))})),this._showBackdrop((()=>this._showElement(t))))}hide(){if(!this._isShown||this._isTransitioning)return;if(j.trigger(this._element,"hide.bs.modal").defaultPrevented)return;this._isShown=!1;const t=this._isAnimated();t&&(this._isTransitioning=!0),this._setEscapeEvent(),this._setResizeEvent(),this._focustrap.deactivate(),this._element.classList.remove(ji),j.off(this._element,Si),j.off(this._dialog,Ii),this._queueCallback((()=>this._hideModal()),this._element,t)}dispose(){[window,this._dialog].forEach((t=>j.off(t,".bs.modal"))),this._backdrop.dispose(),this._focustrap.deactivate(),super.dispose()}handleUpdate(){this._adjustDialog()}_initializeBackDrop(){return new bi({isVisible:Boolean(this._config.backdrop),isAnimated:this._isAnimated()})}_initializeFocusTrap(){return new Ai({trapElement:this._element})}_getConfig(t){return t={...Ci,...U.getDataAttributes(this._element),..."object"==typeof t?t:{}},a(Ti,t,ki),t}_showElement(t){const e=this._isAnimated(),i=V.findOne(".modal-body",this._dialog);this._element.parentNode&&this._element.parentNode.nodeType===Node.ELEMENT_NODE||document.body.append(this._element),this._element.style.display="block",this._element.removeAttribute("aria-hidden"),this._element.setAttribute("aria-modal",!0),this._element.setAttribute("role","dialog"),this._element.scrollTop=0,i&&(i.scrollTop=0),e&&u(this._element),this._element.classList.add(ji),this._queueCallback((()=>{this._config.focus&&this._focustrap.activate(),this._isTransitioning=!1,j.trigger(this._element,"shown.bs.modal",{relatedTarget:t})}),this._dialog,e)}_setEscapeEvent(){this._isShown?j.on(this._element,Ni,(t=>{this._config.keyboard&&t.key===Oi?(t.preventDefault(),this.hide()):this._config.keyboard||t.key!==Oi||this._triggerBackdropTransition()})):j.off(this._element,Ni)}_setResizeEvent(){this._isShown?j.on(window,Di,(()=>this._adjustDialog())):j.off(window,Di)}_hideModal(){this._element.style.display="none",this._element.setAttribute("aria-hidden",!0),this._element.removeAttribute("aria-modal"),this._element.removeAttribute("role"),this._isTransitioning=!1,this._backdrop.hide((()=>{document.body.classList.remove(Pi),this._resetAdjustments(),this._scrollBar.reset(),j.trigger(this._element,Li)}))}_showBackdrop(t){j.on(this._element,Si,(t=>{this._ignoreBackdropClick?this._ignoreBackdropClick=!1:t.target===t.currentTarget&&(!0===this._config.backdrop?this.hide():"static"===this._config.backdrop&&this._triggerBackdropTransition())})),this._backdrop.show(t)}_isAnimated(){return this._element.classList.contains("fade")}_triggerBackdropTransition(){if(j.trigger(this._element,"hidePrevented.bs.modal").defaultPrevented)return;const{classList:t,scrollHeight:e,style:i}=this._element,n=e>document.documentElement.clientHeight;!n&&"hidden"===i.overflowY||t.contains(Mi)||(n||(i.overflowY="hidden"),t.add(Mi),this._queueCallback((()=>{t.remove(Mi),n||this._queueCallback((()=>{i.overflowY=""}),this._dialog)}),this._dialog),this._element.focus())}_adjustDialog(){const t=this._element.scrollHeight>document.documentElement.clientHeight,e=this._scrollBar.getWidth(),i=e>0;(!i&&t&&!m()||i&&!t&&m())&&(this._element.style.paddingLeft=`${e}px`),(i&&!t&&!m()||!i&&t&&m())&&(this._element.style.paddingRight=`${e}px`)}_resetAdjustments(){this._element.style.paddingLeft="",this._element.style.paddingRight=""}static jQueryInterface(t,e){return this.each((function(){const i=Hi.getOrCreateInstance(this,t);if("string"==typeof t){if(void 0===i[t])throw new TypeError(`No method named "${t}"`);i[t](e)}}))}}j.on(document,"click.bs.modal.data-api",'[data-bs-toggle="modal"]',(function(t){const e=n(this);["A","AREA"].includes(this.tagName)&&t.preventDefault(),j.one(e,xi,(t=>{t.defaultPrevented||j.one(e,Li,(()=>{l(this)&&this.focus()}))}));const i=V.findOne(".modal.show");i&&Hi.getInstance(i).hide(),Hi.getOrCreateInstance(e).toggle(this)})),R(Hi),g(Hi);const Bi="offcanvas",Ri={backdrop:!0,keyboard:!0,scroll:!1},Wi={backdrop:"boolean",keyboard:"boolean",scroll:"boolean"},$i="show",zi=".offcanvas.show",qi="hidden.bs.offcanvas";class Fi extends B{constructor(t,e){super(t),this._config=this._getConfig(e),this._isShown=!1,this._backdrop=this._initializeBackDrop(),this._focustrap=this._initializeFocusTrap(),this._addEventListeners()}static get NAME(){return Bi}static get Default(){return Ri}toggle(t){return this._isShown?this.hide():this.show(t)}show(t){this._isShown||j.trigger(this._element,"show.bs.offcanvas",{relatedTarget:t}).defaultPrevented||(this._isShown=!0,this._element.style.visibility="visible",this._backdrop.show(),this._config.scroll||(new fi).hide(),this._element.removeAttribute("aria-hidden"),this._element.setAttribute("aria-modal",!0),this._element.setAttribute("role","dialog"),this._element.classList.add($i),this._queueCallback((()=>{this._config.scroll||this._focustrap.activate(),j.trigger(this._element,"shown.bs.offcanvas",{relatedTarget:t})}),this._element,!0))}hide(){this._isShown&&(j.trigger(this._element,"hide.bs.offcanvas").defaultPrevented||(this._focustrap.deactivate(),this._element.blur(),this._isShown=!1,this._element.classList.remove($i),this._backdrop.hide(),this._queueCallback((()=>{this._element.setAttribute("aria-hidden",!0),this._element.removeAttribute("aria-modal"),this._element.removeAttribute("role"),this._element.style.visibility="hidden",this._config.scroll||(new fi).reset(),j.trigger(this._element,qi)}),this._element,!0)))}dispose(){this._backdrop.dispose(),this._focustrap.deactivate(),super.dispose()}_getConfig(t){return t={...Ri,...U.getDataAttributes(this._element),..."object"==typeof t?t:{}},a(Bi,t,Wi),t}_initializeBackDrop(){return new bi({className:"offcanvas-backdrop",isVisible:this._config.backdrop,isAnimated:!0,rootElement:this._element.parentNode,clickCallback:()=>this.hide()})}_initializeFocusTrap(){return new Ai({trapElement:this._element})}_addEventListeners(){j.on(this._element,"keydown.dismiss.bs.offcanvas",(t=>{this._config.keyboard&&"Escape"===t.key&&this.hide()}))}static jQueryInterface(t){return this.each((function(){const e=Fi.getOrCreateInstance(this,t);if("string"==typeof t){if(void 0===e[t]||t.startsWith("_")||"constructor"===t)throw new TypeError(`No method named "${t}"`);e[t](this)}}))}}j.on(document,"click.bs.offcanvas.data-api",'[data-bs-toggle="offcanvas"]',(function(t){const e=n(this);if(["A","AREA"].includes(this.tagName)&&t.preventDefault(),c(this))return;j.one(e,qi,(()=>{l(this)&&this.focus()}));const i=V.findOne(zi);i&&i!==e&&Fi.getInstance(i).hide(),Fi.getOrCreateInstance(e).toggle(this)})),j.on(window,"load.bs.offcanvas.data-api",(()=>V.find(zi).forEach((t=>Fi.getOrCreateInstance(t).show())))),R(Fi),g(Fi);const Ui=new Set(["background","cite","href","itemtype","longdesc","poster","src","xlink:href"]),Vi=/^(?:(?:https?|mailto|ftp|tel|file|sms):|[^#&/:?]*(?:[#/?]|$))/i,Ki=/^data:(?:image\/(?:bmp|gif|jpeg|jpg|png|tiff|webp)|video\/(?:mpeg|mp4|ogg|webm)|audio\/(?:mp3|oga|ogg|opus));base64,[\d+/a-z]+=*$/i,Xi=(t,e)=>{const i=t.nodeName.toLowerCase();if(e.includes(i))return!Ui.has(i)||Boolean(Vi.test(t.nodeValue)||Ki.test(t.nodeValue));const n=e.filter((t=>t instanceof RegExp));for(let t=0,e=n.length;t{Xi(t,r)||i.removeAttribute(t.nodeName)}))}return n.body.innerHTML}const Qi="tooltip",Gi=new Set(["sanitize","allowList","sanitizeFn"]),Zi={animation:"boolean",template:"string",title:"(string|element|function)",trigger:"string",delay:"(number|object)",html:"boolean",selector:"(string|boolean)",placement:"(string|function)",offset:"(array|string|function)",container:"(string|element|boolean)",fallbackPlacements:"array",boundary:"(string|element)",customClass:"(string|function)",sanitize:"boolean",sanitizeFn:"(null|function)",allowList:"object",popperConfig:"(null|object|function)"},Ji={AUTO:"auto",TOP:"top",RIGHT:m()?"left":"right",BOTTOM:"bottom",LEFT:m()?"right":"left"},tn={animation:!0,template:'',trigger:"hover focus",title:"",delay:0,html:!1,selector:!1,placement:"top",offset:[0,0],container:!1,fallbackPlacements:["top","right","bottom","left"],boundary:"clippingParents",customClass:"",sanitize:!0,sanitizeFn:null,allowList:{"*":["class","dir","id","lang","role",/^aria-[\w-]*$/i],a:["target","href","title","rel"],area:[],b:[],br:[],col:[],code:[],div:[],em:[],hr:[],h1:[],h2:[],h3:[],h4:[],h5:[],h6:[],i:[],img:["src","srcset","alt","title","width","height"],li:[],ol:[],p:[],pre:[],s:[],small:[],span:[],sub:[],sup:[],strong:[],u:[],ul:[]},popperConfig:null},en={HIDE:"hide.bs.tooltip",HIDDEN:"hidden.bs.tooltip",SHOW:"show.bs.tooltip",SHOWN:"shown.bs.tooltip",INSERTED:"inserted.bs.tooltip",CLICK:"click.bs.tooltip",FOCUSIN:"focusin.bs.tooltip",FOCUSOUT:"focusout.bs.tooltip",MOUSEENTER:"mouseenter.bs.tooltip",MOUSELEAVE:"mouseleave.bs.tooltip"},nn="fade",sn="show",on="show",rn="out",an=".tooltip-inner",ln=".modal",cn="hide.bs.modal",hn="hover",dn="focus";class un extends B{constructor(t,e){if(void 0===Fe)throw new TypeError("Bootstrap's tooltips require Popper (https://popper.js.org)");super(t),this._isEnabled=!0,this._timeout=0,this._hoverState="",this._activeTrigger={},this._popper=null,this._config=this._getConfig(e),this.tip=null,this._setListeners()}static get Default(){return tn}static get NAME(){return Qi}static get Event(){return en}static get DefaultType(){return Zi}enable(){this._isEnabled=!0}disable(){this._isEnabled=!1}toggleEnabled(){this._isEnabled=!this._isEnabled}toggle(t){if(this._isEnabled)if(t){const e=this._initializeOnDelegatedTarget(t);e._activeTrigger.click=!e._activeTrigger.click,e._isWithActiveTrigger()?e._enter(null,e):e._leave(null,e)}else{if(this.getTipElement().classList.contains(sn))return void 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t=this.getTipElement();if(j.trigger(this._element,this.constructor.Event.HIDE).defaultPrevented)return;t.classList.remove(sn),"ontouchstart"in document.documentElement&&[].concat(...document.body.children).forEach((t=>j.off(t,"mouseover",d))),this._activeTrigger.click=!1,this._activeTrigger.focus=!1,this._activeTrigger.hover=!1;const e=this.tip.classList.contains(nn);this._queueCallback((()=>{this._isWithActiveTrigger()||(this._hoverState!==on&&t.remove(),this._cleanTipClass(),this._element.removeAttribute("aria-describedby"),j.trigger(this._element,this.constructor.Event.HIDDEN),this._disposePopper())}),this.tip,e),this._hoverState=""}update(){null!==this._popper&&this._popper.update()}isWithContent(){return Boolean(this.getTitle())}getTipElement(){if(this.tip)return this.tip;const t=document.createElement("div");t.innerHTML=this._config.template;const e=t.children[0];return this.setContent(e),e.classList.remove(nn,sn),this.tip=e,this.tip}setContent(t){this._sanitizeAndSetContent(t,this.getTitle(),an)}_sanitizeAndSetContent(t,e,i){const n=V.findOne(i,t);e||!n?this.setElementContent(n,e):n.remove()}setElementContent(t,e){if(null!==t)return o(e)?(e=r(e),void(this._config.html?e.parentNode!==t&&(t.innerHTML="",t.append(e)):t.textContent=e.textContent)):void(this._config.html?(this._config.sanitize&&(e=Yi(e,this._config.allowList,this._config.sanitizeFn)),t.innerHTML=e):t.textContent=e)}getTitle(){const t=this._element.getAttribute("data-bs-original-title")||this._config.title;return this._resolvePossibleFunction(t)}updateAttachment(t){return"right"===t?"end":"left"===t?"start":t}_initializeOnDelegatedTarget(t,e){return e||this.constructor.getOrCreateInstance(t.delegateTarget,this._getDelegateConfig())}_getOffset(){const{offset:t}=this._config;return"string"==typeof t?t.split(",").map((t=>Number.parseInt(t,10))):"function"==typeof t?e=>t(e,this._element):t}_resolvePossibleFunction(t){return"function"==typeof t?t.call(this._element):t}_getPopperConfig(t){const e={placement:t,modifiers:[{name:"flip",options:{fallbackPlacements:this._config.fallbackPlacements}},{name:"offset",options:{offset:this._getOffset()}},{name:"preventOverflow",options:{boundary:this._config.boundary}},{name:"arrow",options:{element:`.${this.constructor.NAME}-arrow`}},{name:"onChange",enabled:!0,phase:"afterWrite",fn:t=>this._handlePopperPlacementChange(t)}],onFirstUpdate:t=>{t.options.placement!==t.placement&&this._handlePopperPlacementChange(t)}};return{...e,..."function"==typeof this._config.popperConfig?this._config.popperConfig(e):this._config.popperConfig}}_addAttachmentClass(t){this.getTipElement().classList.add(`${this._getBasicClassPrefix()}-${this.updateAttachment(t)}`)}_getAttachment(t){return Ji[t.toUpperCase()]}_setListeners(){this._config.trigger.split(" 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this._element.getAttribute("data-bs-original-title");(t||"string"!==e)&&(this._element.setAttribute("data-bs-original-title",t||""),!t||this._element.getAttribute("aria-label")||this._element.textContent||this._element.setAttribute("aria-label",t),this._element.setAttribute("title",""))}_enter(t,e){e=this._initializeOnDelegatedTarget(t,e),t&&(e._activeTrigger["focusin"===t.type?dn:hn]=!0),e.getTipElement().classList.contains(sn)||e._hoverState===on?e._hoverState=on:(clearTimeout(e._timeout),e._hoverState=on,e._config.delay&&e._config.delay.show?e._timeout=setTimeout((()=>{e._hoverState===on&&e.show()}),e._config.delay.show):e.show())}_leave(t,e){e=this._initializeOnDelegatedTarget(t,e),t&&(e._activeTrigger["focusout"===t.type?dn:hn]=e._element.contains(t.relatedTarget)),e._isWithActiveTrigger()||(clearTimeout(e._timeout),e._hoverState=rn,e._config.delay&&e._config.delay.hide?e._timeout=setTimeout((()=>{e._hoverState===rn&&e.hide()}),e._config.delay.hide):e.hide())}_isWithActiveTrigger(){for(const t in this._activeTrigger)if(this._activeTrigger[t])return!0;return!1}_getConfig(t){const e=U.getDataAttributes(this._element);return Object.keys(e).forEach((t=>{Gi.has(t)&&delete e[t]})),(t={...this.constructor.Default,...e,..."object"==typeof t&&t?t:{}}).container=!1===t.container?document.body:r(t.container),"number"==typeof t.delay&&(t.delay={show:t.delay,hide:t.delay}),"number"==typeof t.title&&(t.title=t.title.toString()),"number"==typeof t.content&&(t.content=t.content.toString()),a(Qi,t,this.constructor.DefaultType),t.sanitize&&(t.template=Yi(t.template,t.allowList,t.sanitizeFn)),t}_getDelegateConfig(){const t={};for(const e in this._config)this.constructor.Default[e]!==this._config[e]&&(t[e]=this._config[e]);return t}_cleanTipClass(){const t=this.getTipElement(),e=new RegExp(`(^|\\s)${this._getBasicClassPrefix()}\\S+`,"g"),i=t.getAttribute("class").match(e);null!==i&&i.length>0&&i.map((t=>t.trim())).forEach((e=>t.classList.remove(e)))}_getBasicClassPrefix(){return"bs-tooltip"}_handlePopperPlacementChange(t){const{state:e}=t;e&&(this.tip=e.elements.popper,this._cleanTipClass(),this._addAttachmentClass(this._getAttachment(e.placement)))}_disposePopper(){this._popper&&(this._popper.destroy(),this._popper=null)}static jQueryInterface(t){return this.each((function(){const e=un.getOrCreateInstance(this,t);if("string"==typeof t){if(void 0===e[t])throw new TypeError(`No method named "${t}"`);e[t]()}}))}}g(un);const fn={...un.Default,placement:"right",offset:[0,8],trigger:"click",content:"",template:''},pn={...un.DefaultType,content:"(string|element|function)"},mn={HIDE:"hide.bs.popover",HIDDEN:"hidden.bs.popover",SHOW:"show.bs.popover",SHOWN:"shown.bs.popover",INSERTED:"inserted.bs.popover",CLICK:"click.bs.popover",FOCUSIN:"focusin.bs.popover",FOCUSOUT:"focusout.bs.popover",MOUSEENTER:"mouseenter.bs.popover",MOUSELEAVE:"mouseleave.bs.popover"};class gn extends un{static get Default(){return fn}static get NAME(){return"popover"}static get Event(){return mn}static get DefaultType(){return pn}isWithContent(){return this.getTitle()||this._getContent()}setContent(t){this._sanitizeAndSetContent(t,this.getTitle(),".popover-header"),this._sanitizeAndSetContent(t,this._getContent(),".popover-body")}_getContent(){return this._resolvePossibleFunction(this._config.content)}_getBasicClassPrefix(){return"bs-popover"}static jQueryInterface(t){return this.each((function(){const 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t=this._scrollElement===this._scrollElement.window?"offset":En,e="auto"===this._config.method?t:this._config.method,n=e===En?this._getScrollTop():0;this._offsets=[],this._targets=[],this._scrollHeight=this._getScrollHeight(),V.find(wn,this._config.target).map((t=>{const s=i(t),o=s?V.findOne(s):null;if(o){const t=o.getBoundingClientRect();if(t.width||t.height)return[U[e](o).top+n,s]}return null})).filter((t=>t)).sort(((t,e)=>t[0]-e[0])).forEach((t=>{this._offsets.push(t[0]),this._targets.push(t[1])}))}dispose(){j.off(this._scrollElement,".bs.scrollspy"),super.dispose()}_getConfig(t){return(t={...bn,...U.getDataAttributes(this._element),..."object"==typeof t&&t?t:{}}).target=r(t.target)||document.documentElement,a(_n,t,vn),t}_getScrollTop(){return this._scrollElement===window?this._scrollElement.pageYOffset:this._scrollElement.scrollTop}_getScrollHeight(){return this._scrollElement.scrollHeight||Math.max(document.body.scrollHeight,document.documentElement.scrollHeight)}_getOffsetHeight(){return this._scrollElement===window?window.innerHeight:this._scrollElement.getBoundingClientRect().height}_process(){const t=this._getScrollTop()+this._config.offset,e=this._getScrollHeight(),i=this._config.offset+e-this._getOffsetHeight();if(this._scrollHeight!==e&&this.refresh(),t>=i){const t=this._targets[this._targets.length-1];this._activeTarget!==t&&this._activate(t)}else{if(this._activeTarget&&t0)return this._activeTarget=null,void this._clear();for(let e=this._offsets.length;e--;)this._activeTarget!==this._targets[e]&&t>=this._offsets[e]&&(void 0===this._offsets[e+1]||t`${e}[data-bs-target="${t}"],${e}[href="${t}"]`)),i=V.findOne(e.join(","),this._config.target);i.classList.add(yn),i.classList.contains("dropdown-item")?V.findOne(".dropdown-toggle",i.closest(".dropdown")).classList.add(yn):V.parents(i,".nav, .list-group").forEach((t=>{V.prev(t,".nav-link, .list-group-item").forEach((t=>t.classList.add(yn))),V.prev(t,".nav-item").forEach((t=>{V.children(t,".nav-link").forEach((t=>t.classList.add(yn)))}))})),j.trigger(this._scrollElement,"activate.bs.scrollspy",{relatedTarget:t})}_clear(){V.find(wn,this._config.target).filter((t=>t.classList.contains(yn))).forEach((t=>t.classList.remove(yn)))}static jQueryInterface(t){return this.each((function(){const e=An.getOrCreateInstance(this,t);if("string"==typeof t){if(void 0===e[t])throw new TypeError(`No method named "${t}"`);e[t]()}}))}}j.on(window,"load.bs.scrollspy.data-api",(()=>{V.find('[data-bs-spy="scroll"]').forEach((t=>new An(t)))})),g(An);const Tn="active",On="fade",Cn="show",kn=".active",Ln=":scope > li > .active";class xn extends B{static get NAME(){return"tab"}show(){if(this._element.parentNode&&this._element.parentNode.nodeType===Node.ELEMENT_NODE&&this._element.classList.contains(Tn))return;let t;const e=n(this._element),i=this._element.closest(".nav, .list-group");if(i){const e="UL"===i.nodeName||"OL"===i.nodeName?Ln:kn;t=V.find(e,i),t=t[t.length-1]}const s=t?j.trigger(t,"hide.bs.tab",{relatedTarget:this._element}):null;if(j.trigger(this._element,"show.bs.tab",{relatedTarget:t}).defaultPrevented||null!==s&&s.defaultPrevented)return;this._activate(this._element,i);const o=()=>{j.trigger(t,"hidden.bs.tab",{relatedTarget:this._element}),j.trigger(this._element,"shown.bs.tab",{relatedTarget:t})};e?this._activate(e,e.parentNode,o):o()}_activate(t,e,i){const n=(!e||"UL"!==e.nodeName&&"OL"!==e.nodeName?V.children(e,kn):V.find(Ln,e))[0],s=i&&n&&n.classList.contains(On),o=()=>this._transitionComplete(t,n,i);n&&s?(n.classList.remove(Cn),this._queueCallback(o,t,!0)):o()}_transitionComplete(t,e,i){if(e){e.classList.remove(Tn);const t=V.findOne(":scope > .dropdown-menu 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Dn="toast",Sn="hide",Nn="show",In="showing",Pn={animation:"boolean",autohide:"boolean",delay:"number"},jn={animation:!0,autohide:!0,delay:5e3};class Mn extends B{constructor(t,e){super(t),this._config=this._getConfig(e),this._timeout=null,this._hasMouseInteraction=!1,this._hasKeyboardInteraction=!1,this._setListeners()}static get DefaultType(){return Pn}static get Default(){return jn}static get NAME(){return Dn}show(){j.trigger(this._element,"show.bs.toast").defaultPrevented||(this._clearTimeout(),this._config.animation&&this._element.classList.add("fade"),this._element.classList.remove(Sn),u(this._element),this._element.classList.add(Nn),this._element.classList.add(In),this._queueCallback((()=>{this._element.classList.remove(In),j.trigger(this._element,"shown.bs.toast"),this._maybeScheduleHide()}),this._element,this._config.animation))}hide(){this._element.classList.contains(Nn)&&(j.trigger(this._element,"hide.bs.toast").defaultPrevented||(this._element.classList.add(In),this._queueCallback((()=>{this._element.classList.add(Sn),this._element.classList.remove(In),this._element.classList.remove(Nn),j.trigger(this._element,"hidden.bs.toast")}),this._element,this._config.animation)))}dispose(){this._clearTimeout(),this._element.classList.contains(Nn)&&this._element.classList.remove(Nn),super.dispose()}_getConfig(t){return t={...jn,...U.getDataAttributes(this._element),..."object"==typeof t&&t?t:{}},a(Dn,t,this.constructor.DefaultType),t}_maybeScheduleHide(){this._config.autohide&&(this._hasMouseInteraction||this._hasKeyboardInteraction||(this._timeout=setTimeout((()=>{this.hide()}),this._config.delay)))}_onInteraction(t,e){switch(t.type){case"mouseover":case"mouseout":this._hasMouseInteraction=e;break;case"focusin":case"focusout":this._hasKeyboardInteraction=e}if(e)return void this._clearTimeout();const i=t.relatedTarget;this._element===i||this._element.contains(i)||this._maybeScheduleHide()}_setListeners(){j.on(this._element,"mouseover.bs.toast",(t=>this._onInteraction(t,!0))),j.on(this._element,"mouseout.bs.toast",(t=>this._onInteraction(t,!1))),j.on(this._element,"focusin.bs.toast",(t=>this._onInteraction(t,!0))),j.on(this._element,"focusout.bs.toast",(t=>this._onInteraction(t,!1)))}_clearTimeout(){clearTimeout(this._timeout),this._timeout=null}static jQueryInterface(t){return this.each((function(){const e=Mn.getOrCreateInstance(this,t);if("string"==typeof 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/dev/null +++ b/doc/_book/site_libs/quarto-html/quarto-syntax-highlighting.css @@ -0,0 +1,171 @@ +/* quarto syntax highlight colors */ +:root { + --quarto-hl-ot-color: #003B4F; + --quarto-hl-at-color: #657422; + --quarto-hl-ss-color: #20794D; + --quarto-hl-an-color: #5E5E5E; + --quarto-hl-fu-color: #4758AB; + --quarto-hl-st-color: #20794D; + --quarto-hl-cf-color: #003B4F; + --quarto-hl-op-color: #5E5E5E; + --quarto-hl-er-color: #AD0000; + --quarto-hl-bn-color: #AD0000; + --quarto-hl-al-color: #AD0000; + --quarto-hl-va-color: #111111; + --quarto-hl-bu-color: inherit; + --quarto-hl-ex-color: inherit; + --quarto-hl-pp-color: #AD0000; + --quarto-hl-in-color: #5E5E5E; + --quarto-hl-vs-color: #20794D; + --quarto-hl-wa-color: #5E5E5E; + --quarto-hl-do-color: #5E5E5E; + --quarto-hl-im-color: #00769E; + --quarto-hl-ch-color: #20794D; + --quarto-hl-dt-color: #AD0000; + --quarto-hl-fl-color: #AD0000; + --quarto-hl-co-color: #5E5E5E; + --quarto-hl-cv-color: #5E5E5E; + --quarto-hl-cn-color: #8f5902; + --quarto-hl-sc-color: #5E5E5E; + --quarto-hl-dv-color: #AD0000; + --quarto-hl-kw-color: #003B4F; +} + +/* other quarto variables */ +:root { + --quarto-font-monospace: SFMono-Regular, Menlo, Monaco, Consolas, "Liberation Mono", "Courier New", monospace; +} + +pre > code.sourceCode > span { + color: #003B4F; +} + +code span { + color: #003B4F; +} + +code.sourceCode > span { + color: #003B4F; +} + +div.sourceCode, +div.sourceCode pre.sourceCode { + color: #003B4F; +} + +code span.ot { + color: #003B4F; +} + +code span.at { + color: #657422; +} + +code span.ss { + color: #20794D; +} + +code span.an { + color: #5E5E5E; +} + +code span.fu { + color: #4758AB; +} + +code span.st { + color: #20794D; +} + +code span.cf { + color: #003B4F; +} + +code span.op { + color: #5E5E5E; +} + +code span.er { + color: #AD0000; +} + +code span.bn { + color: #AD0000; +} + +code span.al { + color: #AD0000; +} + +code span.va { + color: #111111; +} + +code span.pp { + color: #AD0000; +} + +code span.in { + color: #5E5E5E; +} + +code span.vs { + color: #20794D; +} + +code span.wa { + color: #5E5E5E; + font-style: italic; +} + +code span.do { + color: #5E5E5E; + font-style: italic; +} + +code span.im { + color: #00769E; +} + +code span.ch { + color: #20794D; +} + +code span.dt { + color: #AD0000; +} + +code span.fl { + color: #AD0000; +} + +code span.co { + color: #5E5E5E; +} + +code span.cv { + color: #5E5E5E; + font-style: italic; +} + +code span.cn { + color: #8f5902; +} + +code span.sc { + color: #5E5E5E; +} + +code span.dv { + color: #AD0000; +} + +code span.kw { + color: #003B4F; +} + +.prevent-inlining { + content: " { + const sibling = el.previousElementSibling; + if (sibling && sibling.tagName === "A") { + return sibling.classList.contains("active"); + } else { + return false; + } + }; + + // fire slideEnter for bootstrap tab activations (for htmlwidget resize behavior) + function fireSlideEnter(e) { + const event = window.document.createEvent("Event"); + event.initEvent("slideenter", true, true); + window.document.dispatchEvent(event); + } + const tabs = window.document.querySelectorAll('a[data-bs-toggle="tab"]'); + tabs.forEach((tab) => { + tab.addEventListener("shown.bs.tab", fireSlideEnter); + }); + + // fire slideEnter for tabby tab activations (for htmlwidget resize behavior) + document.addEventListener("tabby", fireSlideEnter, false); + + // Track scrolling and mark TOC links as active + // get table of contents and sidebar (bail if we don't have at least one) + const tocLinks = tocEl + ? [...tocEl.querySelectorAll("a[data-scroll-target]")] + : []; + const makeActive = (link) => tocLinks[link].classList.add("active"); + const removeActive = (link) => tocLinks[link].classList.remove("active"); + const removeAllActive = () => + [...Array(tocLinks.length).keys()].forEach((link) => removeActive(link)); + + // activate the anchor for a section associated with this TOC entry + tocLinks.forEach((link) => { + link.addEventListener("click", () => { + if (link.href.indexOf("#") !== -1) { + const anchor = link.href.split("#")[1]; + const heading = window.document.querySelector( + `[data-anchor-id=${anchor}]` + ); + if (heading) { + // Add the class + heading.classList.add("reveal-anchorjs-link"); + + // function to show the anchor + const handleMouseout = () => { + heading.classList.remove("reveal-anchorjs-link"); + heading.removeEventListener("mouseout", handleMouseout); + }; + + // add a function to clear the anchor when the user mouses out of it + heading.addEventListener("mouseout", handleMouseout); + } + } + }); + }); + + const sections = tocLinks.map((link) => { + const target = link.getAttribute("data-scroll-target"); + if (target.startsWith("#")) { + return window.document.getElementById(decodeURI(`${target.slice(1)}`)); + } else { + return window.document.querySelector(decodeURI(`${target}`)); + } + }); + + const sectionMargin = 200; + let currentActive = 0; + // track whether we've initialized state the first time + let init = false; + + const updateActiveLink = () => { + // The index from bottom to top (e.g. reversed list) + let sectionIndex = -1; + if ( + window.innerHeight + window.pageYOffset >= + window.document.body.offsetHeight + ) { + sectionIndex = 0; + } else { + sectionIndex = [...sections].reverse().findIndex((section) => { + if (section) { + return window.pageYOffset >= section.offsetTop - sectionMargin; + } else { + return false; + } + }); + } + if (sectionIndex > -1) { + const current = sections.length - sectionIndex - 1; + if (current !== currentActive) { + removeAllActive(); + currentActive = current; + makeActive(current); + if (init) { + window.dispatchEvent(sectionChanged); + } + init = true; + } + } + }; + + const inHiddenRegion = (top, bottom, hiddenRegions) => { + for (const region of hiddenRegions) { + if (top <= region.bottom && bottom >= region.top) { + return true; + } + } + return false; + }; + + const categorySelector = "header.quarto-title-block .quarto-category"; + const activateCategories = (href) => { + // Find any categories + // Surround them with a link pointing back to: + // #category=Authoring + try { + const categoryEls = window.document.querySelectorAll(categorySelector); + for (const categoryEl of categoryEls) { + const categoryText = categoryEl.textContent; + if (categoryText) { + const link = `${href}#category=${encodeURIComponent(categoryText)}`; + const linkEl = window.document.createElement("a"); + linkEl.setAttribute("href", link); + for (const child of categoryEl.childNodes) { + linkEl.append(child); + } + categoryEl.appendChild(linkEl); + } + } + } catch { + // Ignore errors + } + }; + function hasTitleCategories() { + return window.document.querySelector(categorySelector) !== null; + } + + function offsetRelativeUrl(url) { + const offset = getMeta("quarto:offset"); + return offset ? offset + url : url; + } + + function offsetAbsoluteUrl(url) { + const offset = getMeta("quarto:offset"); + const baseUrl = new URL(offset, window.location); + + const projRelativeUrl = url.replace(baseUrl, ""); + if (projRelativeUrl.startsWith("/")) { + return projRelativeUrl; + } else { + return "/" + projRelativeUrl; + } + } + + // read a meta tag value + function getMeta(metaName) { + const metas = window.document.getElementsByTagName("meta"); + for (let i = 0; i < metas.length; i++) { + if (metas[i].getAttribute("name") === metaName) { + return metas[i].getAttribute("content"); + } + } + return ""; + } + + async function findAndActivateCategories() { + const currentPagePath = offsetAbsoluteUrl(window.location.href); + const response = await fetch(offsetRelativeUrl("listings.json")); + if (response.status == 200) { + return response.json().then(function (listingPaths) { + const listingHrefs = []; + for (const listingPath of listingPaths) { + const pathWithoutLeadingSlash = listingPath.listing.substring(1); + for (const item of listingPath.items) { + if ( + item === currentPagePath || + item === currentPagePath + "index.html" + ) { + // Resolve this path against the offset to be sure + // we already are using the correct path to the listing + // (this adjusts the listing urls to be rooted against + // whatever root the page is actually running against) + const relative = offsetRelativeUrl(pathWithoutLeadingSlash); + const baseUrl = window.location; + const resolvedPath = new URL(relative, baseUrl); + listingHrefs.push(resolvedPath.pathname); + break; + } + } + } + + // Look up the tree for a nearby linting and use that if we find one + const nearestListing = findNearestParentListing( + offsetAbsoluteUrl(window.location.pathname), + listingHrefs + ); + if (nearestListing) { + activateCategories(nearestListing); + } else { + // See if the referrer is a listing page for this item + const referredRelativePath = offsetAbsoluteUrl(document.referrer); + const referrerListing = listingHrefs.find((listingHref) => { + const isListingReferrer = + listingHref === referredRelativePath || + listingHref === referredRelativePath + "index.html"; + return isListingReferrer; + }); + + if (referrerListing) { + // Try to use the referrer if possible + activateCategories(referrerListing); + } else if (listingHrefs.length > 0) { + // Otherwise, just fall back to the first listing + activateCategories(listingHrefs[0]); + } + } + }); + } + } + if (hasTitleCategories()) { + findAndActivateCategories(); + } + + const findNearestParentListing = (href, listingHrefs) => { + if (!href || !listingHrefs) { + return undefined; + } + // Look up the tree for a nearby linting and use that if we find one + const relativeParts = href.substring(1).split("/"); + while (relativeParts.length > 0) { + const path = relativeParts.join("/"); + for (const listingHref of listingHrefs) { + if (listingHref.startsWith(path)) { + return listingHref; + } + } + relativeParts.pop(); + } + + return undefined; + }; + + const manageSidebarVisiblity = (el, placeholderDescriptor) => { + let isVisible = true; + + return (hiddenRegions) => { + if (el === null) { + return; + } + + // Find the last element of the TOC + const lastChildEl = el.lastElementChild; + + if (lastChildEl) { + // Find the top and bottom o the element that is being managed + const elTop = el.offsetTop; + const elBottom = + elTop + lastChildEl.offsetTop + lastChildEl.offsetHeight; + + // Converts the sidebar to a menu + const convertToMenu = () => { + for (const child of el.children) { + child.style.opacity = 0; + child.style.overflow = "hidden"; + } + + const toggleContainer = window.document.createElement("div"); + toggleContainer.style.width = "100%"; + toggleContainer.classList.add("zindex-over-content"); + toggleContainer.classList.add("quarto-sidebar-toggle"); + toggleContainer.classList.add("headroom-target"); // Marks this to be managed by headeroom + toggleContainer.id = placeholderDescriptor.id; + toggleContainer.style.position = "fixed"; + + const toggleIcon = window.document.createElement("i"); + toggleIcon.classList.add("quarto-sidebar-toggle-icon"); + toggleIcon.classList.add("bi"); + toggleIcon.classList.add("bi-caret-down-fill"); + + const toggleTitle = window.document.createElement("div"); + const titleEl = window.document.body.querySelector( + placeholderDescriptor.titleSelector + ); + if (titleEl) { + toggleTitle.append(titleEl.innerText, toggleIcon); + } + toggleTitle.classList.add("zindex-over-content"); + toggleTitle.classList.add("quarto-sidebar-toggle-title"); + toggleContainer.append(toggleTitle); + + const toggleContents = window.document.createElement("div"); + toggleContents.classList = el.classList; + toggleContents.classList.add("zindex-over-content"); + toggleContents.classList.add("quarto-sidebar-toggle-contents"); + for (const child of el.children) { + if (child.id === "toc-title") { + continue; + } + + const clone = child.cloneNode(true); + clone.style.opacity = 1; + clone.style.display = null; + toggleContents.append(clone); + } + toggleContents.style.height = "0px"; + toggleContainer.append(toggleContents); + el.parentElement.prepend(toggleContainer); + + // Process clicks + let tocShowing = false; + // Allow the caller to control whether this is dismissed + // when it is clicked (e.g. sidebar navigation supports + // opening and closing the nav tree, so don't dismiss on click) + const clickEl = placeholderDescriptor.dismissOnClick + ? toggleContainer + : toggleTitle; + + const closeToggle = () => { + if (tocShowing) { + toggleContainer.classList.remove("expanded"); + toggleContents.style.height = "0px"; + tocShowing = false; + } + }; + + const positionToggle = () => { + // position the element (top left of parent, same width as parent) + const elRect = el.getBoundingClientRect(); + toggleContainer.style.left = `${elRect.left}px`; + toggleContainer.style.top = `${elRect.top}px`; + toggleContainer.style.width = `${elRect.width}px`; + }; + + // Get rid of any expanded toggle if the user scrolls + window.document.addEventListener( + "scroll", + throttle(() => { + closeToggle(); + }, 50) + ); + + // Handle positioning of the toggle + window.addEventListener( + "resize", + throttle(() => { + positionToggle(); + }, 50) + ); + positionToggle(); + + // Process the click + clickEl.onclick = () => { + if (!tocShowing) { + toggleContainer.classList.add("expanded"); + toggleContents.style.height = null; + tocShowing = true; + } else { + closeToggle(); + } + }; + }; + + // Converts a sidebar from a menu back to a sidebar + const convertToSidebar = () => { + for (const child of el.children) { + child.style.opacity = 1; + child.style.overflow = null; + } + + const placeholderEl = window.document.getElementById( + placeholderDescriptor.id + ); + if (placeholderEl) { + placeholderEl.remove(); + } + + el.classList.remove("rollup"); + }; + + if (isReaderMode()) { + convertToMenu(); + isVisible = false; + } else { + if (!isVisible) { + // If the element is current not visible reveal if there are + // no conflicts with overlay regions + if (!inHiddenRegion(elTop, elBottom, hiddenRegions)) { + convertToSidebar(); + isVisible = true; + } + } else { + // If the element is visible, hide it if it conflicts with overlay regions + // and insert a placeholder toggle (or if we're in reader mode) + if (inHiddenRegion(elTop, elBottom, hiddenRegions)) { + convertToMenu(); + isVisible = false; + } + } + } + } + }; + }; + + // Find any conflicting margin elements and add margins to the + // top to prevent overlap + const marginChildren = window.document.querySelectorAll( + ".column-margin.column-container > * " + ); + + nexttick(() => { + let lastBottom = 0; + for (const marginChild of marginChildren) { + const top = marginChild.getBoundingClientRect().top + window.scrollY; + if (top < lastBottom) { + const margin = lastBottom - top; + marginChild.style.marginTop = `${margin}px`; + } + const styles = window.getComputedStyle(marginChild); + const marginTop = parseFloat(styles["marginTop"]); + + lastBottom = top + marginChild.getBoundingClientRect().height + marginTop; + } + }); + + // Manage the visibility of the toc and the sidebar + const marginScrollVisibility = manageSidebarVisiblity(marginSidebarEl, { + id: "quarto-toc-toggle", + titleSelector: "#toc-title", + dismissOnClick: true, + }); + const sidebarScrollVisiblity = manageSidebarVisiblity(sidebarEl, { + id: "quarto-sidebarnav-toggle", + titleSelector: ".title", + dismissOnClick: false, + }); + let tocLeftScrollVisibility; + if (leftTocEl) { + tocLeftScrollVisibility = manageSidebarVisiblity(leftTocEl, { + id: "quarto-lefttoc-toggle", + titleSelector: "#toc-title", + dismissOnClick: true, + }); + } + + // Find the first element that uses formatting in special columns + const conflictingEls = window.document.body.querySelectorAll( + '[class^="column-"], [class*=" column-"], aside, [class*="margin-caption"], [class*=" margin-caption"], [class*="margin-ref"], [class*=" margin-ref"]' + ); + + // Filter all the possibly conflicting elements into ones + // the do conflict on the left or ride side + const arrConflictingEls = Array.from(conflictingEls); + const leftSideConflictEls = arrConflictingEls.filter((el) => { + if (el.tagName === "ASIDE") { + return false; + } + return Array.from(el.classList).find((className) => { + return ( + className !== "column-body" && + className.startsWith("column-") && + !className.endsWith("right") && + !className.endsWith("container") && + className !== "column-margin" + ); + }); + }); + const rightSideConflictEls = arrConflictingEls.filter((el) => { + if (el.tagName === "ASIDE") { + return true; + } + + const hasMarginCaption = Array.from(el.classList).find((className) => { + return className == "margin-caption"; + }); + if (hasMarginCaption) { + return true; + } + + return Array.from(el.classList).find((className) => { + return ( + className !== "column-body" && + !className.endsWith("container") && + className.startsWith("column-") && + !className.endsWith("left") + ); + }); + }); + + const kOverlapPaddingSize = 10; + function toRegions(els) { + return els.map((el) => { + const top = + el.getBoundingClientRect().top + + document.documentElement.scrollTop - + kOverlapPaddingSize; + return { + top, + bottom: top + el.scrollHeight + 2 * kOverlapPaddingSize, + }; + }); + } + + const hideOverlappedSidebars = () => { + marginScrollVisibility(toRegions(rightSideConflictEls)); + sidebarScrollVisiblity(toRegions(leftSideConflictEls)); + if (tocLeftScrollVisibility) { + tocLeftScrollVisibility(toRegions(leftSideConflictEls)); + } + }; + + window.quartoToggleReader = () => { + // Applies a slow class (or removes it) + // to update the transition speed + const slowTransition = (slow) => { + const manageTransition = (id, slow) => { + const el = document.getElementById(id); + if (el) { + if (slow) { + el.classList.add("slow"); + } else { + el.classList.remove("slow"); + } + } + }; + + manageTransition("TOC", slow); + manageTransition("quarto-sidebar", slow); + }; + + const readerMode = !isReaderMode(); + setReaderModeValue(readerMode); + + // If we're entering reader mode, slow the transition + if (readerMode) { + slowTransition(readerMode); + } + highlightReaderToggle(readerMode); + hideOverlappedSidebars(); + + // If we're exiting reader mode, restore the non-slow transition + if (!readerMode) { + slowTransition(!readerMode); + } + }; + + const highlightReaderToggle = (readerMode) => { + const els = document.querySelectorAll(".quarto-reader-toggle"); + if (els) { + els.forEach((el) => { + if (readerMode) { + el.classList.add("reader"); + } else { + el.classList.remove("reader"); + } + }); + } + }; + + const setReaderModeValue = (val) => { + if (window.location.protocol !== "file:") { + window.localStorage.setItem("quarto-reader-mode", val); + } else { + localReaderMode = val; + } + }; + + const isReaderMode = () => { + if (window.location.protocol !== "file:") { + return window.localStorage.getItem("quarto-reader-mode") === "true"; + } else { + return localReaderMode; + } + }; + let localReaderMode = null; + + // Walk the TOC and collapse/expand nodes + // Nodes are expanded if: + // - they are top level + // - they have children that are 'active' links + // - they are directly below an link that is 'active' + const walk = (el, depth) => { + // Tick depth when we enter a UL + if (el.tagName === "UL") { + depth = depth + 1; + } + + // It this is active link + let isActiveNode = false; + if (el.tagName === "A" && el.classList.contains("active")) { + isActiveNode = true; + } + + // See if there is an active child to this element + let hasActiveChild = false; + for (child of el.children) { + hasActiveChild = walk(child, depth) || hasActiveChild; + } + + // Process the collapse state if this is an UL + if (el.tagName === "UL") { + if (depth === 1 || hasActiveChild || prevSiblingIsActiveLink(el)) { + el.classList.remove("collapse"); + } else { + el.classList.add("collapse"); + } + + // untick depth when we leave a UL + depth = depth - 1; + } + return hasActiveChild || isActiveNode; + }; + + // walk the TOC and expand / collapse any items that should be shown + + if (tocEl) { + walk(tocEl, 0); + updateActiveLink(); + } + + // Throttle the scroll event and walk peridiocally + window.document.addEventListener( + "scroll", + throttle(() => { + if (tocEl) { + updateActiveLink(); + walk(tocEl, 0); + } + if (!isReaderMode()) { + hideOverlappedSidebars(); + } + }, 5) + ); + window.addEventListener( + "resize", + throttle(() => { + if (!isReaderMode()) { + hideOverlappedSidebars(); + } + }, 10) + ); + hideOverlappedSidebars(); + highlightReaderToggle(isReaderMode()); +}); + +// grouped tabsets +window.addEventListener("pageshow", (_event) => { + function getTabSettings() { + const data = localStorage.getItem("quarto-persistent-tabsets-data"); + if (!data) { + localStorage.setItem("quarto-persistent-tabsets-data", "{}"); + return {}; + } + if (data) { + return JSON.parse(data); + } + } + + function setTabSettings(data) { + localStorage.setItem( + "quarto-persistent-tabsets-data", + JSON.stringify(data) + ); + } + + function setTabState(groupName, groupValue) { + const data = getTabSettings(); + data[groupName] = groupValue; + setTabSettings(data); + } + + function toggleTab(tab, active) { + const tabPanelId = tab.getAttribute("aria-controls"); + const tabPanel = document.getElementById(tabPanelId); + if (active) { + tab.classList.add("active"); + tabPanel.classList.add("active"); + } else { + tab.classList.remove("active"); + tabPanel.classList.remove("active"); + } + } + + function toggleAll(selectedGroup, selectorsToSync) { + for (const [thisGroup, tabs] of Object.entries(selectorsToSync)) { + const active = selectedGroup === thisGroup; + for (const tab of tabs) { + toggleTab(tab, active); + } + } + } + + function findSelectorsToSyncByLanguage() { + const result = {}; + const tabs = Array.from( + document.querySelectorAll(`div[data-group] a[id^='tabset-']`) + ); + for (const item of tabs) { + const div = item.parentElement.parentElement.parentElement; + const group = div.getAttribute("data-group"); + if (!result[group]) { + result[group] = {}; + } + const selectorsToSync = result[group]; + const value = item.innerHTML; + if (!selectorsToSync[value]) { + selectorsToSync[value] = []; + } + selectorsToSync[value].push(item); + } + return result; + } + + function setupSelectorSync() { + const selectorsToSync = findSelectorsToSyncByLanguage(); + Object.entries(selectorsToSync).forEach(([group, tabSetsByValue]) => { + Object.entries(tabSetsByValue).forEach(([value, items]) => { + items.forEach((item) => { + item.addEventListener("click", (_event) => { + setTabState(group, value); + toggleAll(value, selectorsToSync[group]); + }); + }); + }); + }); + return selectorsToSync; + } + + const selectorsToSync = setupSelectorSync(); + for (const [group, selectedName] of Object.entries(getTabSettings())) { + const selectors = selectorsToSync[group]; + // it's possible that stale state gives us empty selections, so we explicitly check here. + if (selectors) { + toggleAll(selectedName, selectors); + } + } +}); + +function throttle(func, wait) { + let waiting = false; + return function () { + if (!waiting) { + func.apply(this, arguments); + waiting = true; + setTimeout(function () { + waiting = false; + }, wait); + } + }; +} + +function nexttick(func) { + return setTimeout(func, 0); +} diff --git a/doc/_book/site_libs/quarto-html/tippy.css b/doc/_book/site_libs/quarto-html/tippy.css new file mode 100644 index 0000000..e6ae635 --- /dev/null +++ b/doc/_book/site_libs/quarto-html/tippy.css @@ -0,0 +1 @@ +.tippy-box[data-animation=fade][data-state=hidden]{opacity:0}[data-tippy-root]{max-width:calc(100vw - 10px)}.tippy-box{position:relative;background-color:#333;color:#fff;border-radius:4px;font-size:14px;line-height:1.4;white-space:normal;outline:0;transition-property:transform,visibility,opacity}.tippy-box[data-placement^=top]>.tippy-arrow{bottom:0}.tippy-box[data-placement^=top]>.tippy-arrow:before{bottom:-7px;left:0;border-width:8px 8px 0;border-top-color:initial;transform-origin:center top}.tippy-box[data-placement^=bottom]>.tippy-arrow{top:0}.tippy-box[data-placement^=bottom]>.tippy-arrow:before{top:-7px;left:0;border-width:0 8px 8px;border-bottom-color:initial;transform-origin:center bottom}.tippy-box[data-placement^=left]>.tippy-arrow{right:0}.tippy-box[data-placement^=left]>.tippy-arrow:before{border-width:8px 0 8px 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--- /dev/null +++ b/doc/_book/site_libs/quarto-nav/quarto-nav.js @@ -0,0 +1,221 @@ +const headroomChanged = new CustomEvent("quarto-hrChanged", { + detail: {}, + bubbles: true, + cancelable: false, + composed: false, +}); + +window.document.addEventListener("DOMContentLoaded", function () { + let init = false; + + function throttle(func, wait) { + var timeout; + return function () { + const context = this; + const args = arguments; + const later = function () { + clearTimeout(timeout); + timeout = null; + func.apply(context, args); + }; + + if (!timeout) { + timeout = setTimeout(later, wait); + } + }; + } + + function headerOffset() { + // Set an offset if there is are fixed top navbar + const headerEl = window.document.querySelector("header.fixed-top"); + if (headerEl) { + return headerEl.clientHeight; + } else { + return 0; + } + } + + function footerOffset() { + const footerEl = window.document.querySelector("footer.footer"); + if (footerEl) { + return footerEl.clientHeight; + } else { + return 0; + } + } + + function updateDocumentOffsetWithoutAnimation() { + updateDocumentOffset(false); + } + + function updateDocumentOffset(animated) { + // set body offset + const topOffset = headerOffset(); + const bodyOffset = topOffset + footerOffset(); + const bodyEl = window.document.body; + bodyEl.setAttribute("data-bs-offset", topOffset); + bodyEl.style.paddingTop = topOffset + "px"; + + // deal with sidebar offsets + const sidebars = window.document.querySelectorAll( + ".sidebar, .headroom-target" + ); + sidebars.forEach((sidebar) => { + if (!animated) { + sidebar.classList.add("notransition"); + // Remove the no transition class after the animation has time to complete + setTimeout(function () { + sidebar.classList.remove("notransition"); + }, 201); + } + + if (window.Headroom && sidebar.classList.contains("sidebar-unpinned")) { + sidebar.style.top = "0"; + sidebar.style.maxHeight = "100vh"; + } else { + sidebar.style.top = topOffset + "px"; + sidebar.style.maxHeight = "calc(100vh - " + topOffset + "px)"; + } + }); + + // allow space for footer + const mainContainer = window.document.querySelector(".quarto-container"); + if (mainContainer) { + mainContainer.style.minHeight = "calc(100vh - " + bodyOffset + "px)"; + } + + // link offset + let linkStyle = window.document.querySelector("#quarto-target-style"); + if (!linkStyle) { + linkStyle = window.document.createElement("style"); + window.document.head.appendChild(linkStyle); + } + while (linkStyle.firstChild) { + linkStyle.removeChild(linkStyle.firstChild); + } + if (topOffset > 0) { + linkStyle.appendChild( + window.document.createTextNode(` + section:target::before { + content: ""; + display: block; + height: ${topOffset}px; + margin: -${topOffset}px 0 0; + }`) + ); + } + if (init) { + window.dispatchEvent(headroomChanged); + } + init = true; + } + + // initialize headroom + var header = window.document.querySelector("#quarto-header"); + if (header && window.Headroom) { + const headroom = new window.Headroom(header, { + tolerance: 5, + onPin: function () { + const sidebars = window.document.querySelectorAll( + ".sidebar, .headroom-target" + ); + sidebars.forEach((sidebar) => { + sidebar.classList.remove("sidebar-unpinned"); + }); + updateDocumentOffset(); + }, + onUnpin: function () { + const sidebars = window.document.querySelectorAll( + ".sidebar, .headroom-target" + ); + sidebars.forEach((sidebar) => { + sidebar.classList.add("sidebar-unpinned"); + }); + updateDocumentOffset(); + }, + }); + headroom.init(); + + let frozen = false; + window.quartoToggleHeadroom = function () { + if (frozen) { + headroom.unfreeze(); + frozen = false; + } else { + headroom.freeze(); + frozen = true; + } + }; + } + + // Observe size changed for the header + const headerEl = window.document.querySelector("header.fixed-top"); + if (headerEl && window.ResizeObserver) { + const observer = new window.ResizeObserver( + updateDocumentOffsetWithoutAnimation + ); + 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item into view, if necessary + const navSidebar = window.document.querySelector("nav#quarto-sidebar"); + if (navSidebar) { + // Find the active item + const activeItem = navSidebar.querySelector("li.sidebar-item a.active"); + if (activeItem) { + // Wait for the scroll height and height to resolve by observing size changes on the + // nav element that is scrollable + const resizeObserver = new ResizeObserver((_entries) => { + // The bottom of the element + const elBottom = activeItem.offsetTop; + const viewBottom = navSidebar.scrollTop + navSidebar.clientHeight; + + // The element height and scroll height are the same, then we are still loading + if (viewBottom !== navSidebar.scrollHeight) { + // Determine if the item isn't visible and scroll to it + if (elBottom >= viewBottom) { + navSidebar.scrollTop = elBottom; + } + + // stop observing now since we've completed the scroll + resizeObserver.unobserve(navSidebar); + } + }); + resizeObserver.observe(navSidebar); + } + } + } +}); diff 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Apache Software License 2.0 + * + * http://www.apache.org/licenses/LICENSE-2.0 + */ +var e,t;e=this,t=function(){"use strict";function e(e,t){var n=Object.keys(e);if(Object.getOwnPropertySymbols){var r=Object.getOwnPropertySymbols(e);t&&(r=r.filter((function(t){return Object.getOwnPropertyDescriptor(e,t).enumerable}))),n.push.apply(n,r)}return n}function t(t){for(var n=1;ne.length)&&(t=e.length);for(var n=0,r=new Array(t);n0&&void 0!==arguments[0]?arguments[0]:1,t=arguments.length>1&&void 0!==arguments[1]?arguments[1]:3,n=new Map,r=Math.pow(10,t);return{get:function(t){var i=t.match(C).length;if(n.has(i))return n.get(i);var o=1/Math.pow(i,.5*e),c=parseFloat(Math.round(o*r)/r);return n.set(i,c),c},clear:function(){n.clear()}}}var $=function(){function e(){var t=arguments.length>0&&void 0!==arguments[0]?arguments[0]:{},n=t.getFn,i=void 0===n?I.getFn:n,o=t.fieldNormWeight,c=void 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a/doc/_book/site_libs/quarto-search/quarto-search.js b/doc/_book/site_libs/quarto-search/quarto-search.js new file mode 100644 index 0000000..6fd4b5b --- /dev/null +++ b/doc/_book/site_libs/quarto-search/quarto-search.js @@ -0,0 +1,1123 @@ +const kQueryArg = "q"; +const kResultsArg = "show-results"; + +// If items don't provide a URL, then both the navigator and the onSelect +// function aren't called (and therefore, the default implementation is used) +// +// We're using this sentinel URL to signal to those handlers that this +// item is a more item (along with the type) and can be handled appropriately +const kItemTypeMoreHref = "0767FDFD-0422-4E5A-BC8A-3BE11E5BBA05"; + +window.document.addEventListener("DOMContentLoaded", function (_event) { + // Ensure that search is available on this page. If it isn't, + // should return early and not do anything + var searchEl = window.document.getElementById("quarto-search"); + if (!searchEl) return; + + const { autocomplete } = window["@algolia/autocomplete-js"]; + + let quartoSearchOptions = {}; + let language = {}; + const searchOptionEl = window.document.getElementById( + "quarto-search-options" + ); + if (searchOptionEl) { + const jsonStr = searchOptionEl.textContent; + quartoSearchOptions = JSON.parse(jsonStr); + language = quartoSearchOptions.language; + } + + // note the search mode + if (quartoSearchOptions.type === "overlay") { + searchEl.classList.add("type-overlay"); + } else { + searchEl.classList.add("type-textbox"); + } + + // Used to determine highlighting behavior for this page + // A `q` query param is expected when the user follows a search + // to this page + const currentUrl = new URL(window.location); + const query = currentUrl.searchParams.get(kQueryArg); + const showSearchResults = currentUrl.searchParams.get(kResultsArg); + const mainEl = window.document.querySelector("main"); + + // highlight matches on the page + if (query !== null && mainEl) { + // perform any highlighting + highlight(query, mainEl); + + // fix up the URL to remove the q query param + const replacementUrl = new URL(window.location); + replacementUrl.searchParams.delete(kQueryArg); + window.history.replaceState({}, "", replacementUrl); + } + + // function to clear highlighting on the page when the search query changes + // (e.g. if the user edits the query or clears it) + let highlighting = true; + const resetHighlighting = (searchTerm) => { + if (mainEl && highlighting && query !== null && searchTerm !== query) { + clearHighlight(query, mainEl); + highlighting = false; + } + }; + + // Clear search highlighting when the user scrolls sufficiently + const resetFn = () => { + resetHighlighting(""); + window.removeEventListener("quarto-hrChanged", resetFn); + window.removeEventListener("quarto-sectionChanged", resetFn); + }; + + // Register this event after the initial scrolling and settling of events + // on the page + window.addEventListener("quarto-hrChanged", resetFn); + window.addEventListener("quarto-sectionChanged", resetFn); + + // Responsively switch to overlay mode if the search is present on the navbar + // Note that switching the sidebar to overlay mode requires more coordinate (not just + // the media query since we generate different HTML for sidebar overlays than we do + // for sidebar input UI) + const detachedMediaQuery = + quartoSearchOptions.type === "overlay" + ? "all" + : quartoSearchOptions.location === "navbar" + ? "(max-width: 991px)" + : "none"; + + // If configured, include the analytics client to send insights + const plugins = configurePlugins(quartoSearchOptions); + + let lastState = null; + const { setIsOpen } = autocomplete({ + container: searchEl, + detachedMediaQuery: detachedMediaQuery, + defaultActiveItemId: 0, + panelContainer: "#quarto-search-results", + panelPlacement: quartoSearchOptions["panel-placement"], + debug: false, + plugins, + classNames: { + form: "d-flex", + }, + translations: { + clearButtonTitle: language["search-clear-button-title"], + detachedCancelButtonText: language["search-detached-cancel-button-title"], + submitButtonTitle: language["search-submit-button-title"], + }, + initialState: { + query, + }, + getItemUrl({ item }) { + return item.href; + }, + onStateChange({ state }) { + // Perhaps reset highlighting + resetHighlighting(state.query); + + // If the panel just opened, ensure the panel is positioned properly + if (state.isOpen) { + if (lastState && !lastState.isOpen) { + setTimeout(() => { + positionPanel(quartoSearchOptions["panel-placement"]); + }, 150); + } + } + + // Perhaps show the copy link + showCopyLink(state.query, quartoSearchOptions); + + lastState = state; + }, + reshape({ sources, state }) { + return sources.map((source) => { + try { + const items = source.getItems(); + + // Validate the items + validateItems(items); + + // group the items by document + const groupedItems = new Map(); + items.forEach((item) => { + const hrefParts = item.href.split("#"); + const baseHref = hrefParts[0]; + const isDocumentItem = hrefParts.length === 1; + + const items = groupedItems.get(baseHref); + if (!items) { + groupedItems.set(baseHref, [item]); + } else { + // If the href for this item matches the document + // exactly, place this item first as it is the item that represents + // the document itself + if (isDocumentItem) { + items.unshift(item); + } else { + items.push(item); + } + groupedItems.set(baseHref, items); + } + }); + + const reshapedItems = []; + let count = 1; + for (const [_key, value] of groupedItems) { + const firstItem = value[0]; + reshapedItems.push({ + ...firstItem, + type: kItemTypeDoc, + }); + + const collapseMatches = quartoSearchOptions["collapse-after"]; + const collapseCount = + typeof collapseMatches === "number" ? collapseMatches : 1; + + if (value.length > 1) { + const target = `search-more-${count}`; + const isExpanded = + state.context.expanded && + state.context.expanded.includes(target); + + const remainingCount = value.length - collapseCount; + + for (let i = 1; i < value.length; i++) { + if (collapseMatches && i === collapseCount) { + reshapedItems.push({ + target, + title: isExpanded + ? language["search-hide-matches-text"] + : remainingCount === 1 + ? `${remainingCount} ${language["search-more-match-text"]}` + : `${remainingCount} ${language["search-more-matches-text"]}`, + type: kItemTypeMore, + href: kItemTypeMoreHref, + }); + } + + if (isExpanded || !collapseMatches || i < collapseCount) { + reshapedItems.push({ + ...value[i], + type: kItemTypeItem, + target, + }); + } + } + } + count += 1; + } + + return { + ...source, + getItems() { + return reshapedItems; + }, + }; + } catch (error) { + // Some form of error occurred + return { + ...source, + getItems() { + return [ + { + title: error.name || "An Error Occurred While Searching", + text: + error.message || + "An unknown error occurred while attempting to perform the requested search.", + type: kItemTypeError, + }, + ]; + }, + }; + } + }); + }, + navigator: { + navigate({ itemUrl }) { + if (itemUrl !== offsetURL(kItemTypeMoreHref)) { + window.location.assign(itemUrl); + } + }, + navigateNewTab({ itemUrl }) { + if (itemUrl !== offsetURL(kItemTypeMoreHref)) { + const windowReference = window.open(itemUrl, "_blank", "noopener"); + if (windowReference) { + windowReference.focus(); + } + } + }, + navigateNewWindow({ itemUrl }) { + if (itemUrl !== offsetURL(kItemTypeMoreHref)) { + window.open(itemUrl, "_blank", "noopener"); + } + }, + }, + getSources({ state, setContext, setActiveItemId, refresh }) { + return [ + { + sourceId: "documents", + getItemUrl({ item }) { + if (item.href) { + return offsetURL(item.href); + } else { + return undefined; + } + }, + onSelect({ + item, + state, + setContext, + setIsOpen, + setActiveItemId, + refresh, + }) { + if (item.type === kItemTypeMore) { + toggleExpanded(item, state, setContext, setActiveItemId, refresh); + + // Toggle more + setIsOpen(true); + } + }, + getItems({ query }) { + const limit = quartoSearchOptions.limit; + if (quartoSearchOptions.algolia) { + return algoliaSearch(query, limit, quartoSearchOptions.algolia); + } else { + // Fuse search options + const fuseSearchOptions = { + isCaseSensitive: false, + shouldSort: true, + minMatchCharLength: 2, + limit: limit, + }; + + return readSearchData().then(function (fuse) { + return fuseSearch(query, fuse, fuseSearchOptions); + }); + } + }, + templates: { + noResults({ createElement }) { + return createElement( + "div", + { class: "quarto-search-no-results" }, + language["search-no-results-text"] + ); + }, + header({ items, createElement }) { + // count the documents + const count = items.filter((item) => { + return item.type === kItemTypeDoc; + }).length; + + if (count > 0) { + return createElement( + "div", + { class: "search-result-header" }, + `${count} ${language["search-matching-documents-text"]}` + ); + } else { + return createElement( + "div", + { class: "search-result-header-no-results" }, + `` + ); + } + }, + footer({ _items, createElement }) { + if ( + quartoSearchOptions.algolia && + quartoSearchOptions.algolia["show-logo"] + ) { + const libDir = quartoSearchOptions.algolia["libDir"]; + const logo = createElement("img", { + src: offsetURL( + `${libDir}/quarto-search/search-by-algolia.svg` + ), + class: "algolia-search-logo", + }); + return createElement( + "a", + { href: "http://www.algolia.com/" }, + logo + ); + } + }, + + item({ item, createElement }) { + return renderItem( + item, + createElement, + state, + setActiveItemId, + setContext, + refresh + ); + }, + }, + }, + ]; + }, + }); + + // Remove the labeleledby attribute since it is pointing + // to a non-existent label + if (quartoSearchOptions.type === "overlay") { + const inputEl = window.document.querySelector( + "#quarto-search .aa-Autocomplete" + ); + if (inputEl) { + inputEl.removeAttribute("aria-labelledby"); + } + } + + // If the main document scrolls dismiss the search results + // (otherwise, since they're floating in the document they can scroll with the document) + window.document.body.onscroll = () => { + setIsOpen(false); + }; + + if (showSearchResults) { + setIsOpen(true); + focusSearchInput(); + } +}); + +function configurePlugins(quartoSearchOptions) { + const autocompletePlugins = []; + const algoliaOptions = quartoSearchOptions.algolia; + if ( + algoliaOptions && + algoliaOptions["analytics-events"] && + algoliaOptions["search-only-api-key"] && + algoliaOptions["application-id"] + ) { + const apiKey = algoliaOptions["search-only-api-key"]; + const appId = algoliaOptions["application-id"]; + + // Aloglia insights may not be loaded because they require cookie consent + // Use deferred loading so events will start being recorded when/if consent + // is granted. + const algoliaInsightsDeferredPlugin = deferredLoadPlugin(() => { + if ( + window.aa && + window["@algolia/autocomplete-plugin-algolia-insights"] + ) { + window.aa("init", { + appId, + apiKey, + useCookie: true, + }); + + const { createAlgoliaInsightsPlugin } = + window["@algolia/autocomplete-plugin-algolia-insights"]; + // Register the insights client + const algoliaInsightsPlugin = createAlgoliaInsightsPlugin({ + insightsClient: window.aa, + onItemsChange({ insights, insightsEvents }) { + const events = insightsEvents.map((event) => { + const maxEvents = event.objectIDs.slice(0, 20); + return { + ...event, + objectIDs: maxEvents, + }; + }); + + insights.viewedObjectIDs(...events); + }, + }); + return algoliaInsightsPlugin; + } + }); + + // Add the plugin + autocompletePlugins.push(algoliaInsightsDeferredPlugin); + return autocompletePlugins; + } +} + +// For plugins that may not load immediately, create a wrapper +// plugin and forward events and plugin data once the plugin +// is initialized. This is useful for cases like cookie consent +// which may prevent the analytics insights event plugin from initializing +// immediately. +function deferredLoadPlugin(createPlugin) { + let plugin = undefined; + let subscribeObj = undefined; + const wrappedPlugin = () => { + if (!plugin && subscribeObj) { + plugin = createPlugin(); + if (plugin && plugin.subscribe) { + plugin.subscribe(subscribeObj); + } + } + return plugin; + }; + + return { + subscribe: (obj) => { + subscribeObj = obj; + }, + onStateChange: (obj) => { + const plugin = wrappedPlugin(); + if (plugin && plugin.onStateChange) { + plugin.onStateChange(obj); + } + }, + onSubmit: (obj) => { + const plugin = wrappedPlugin(); + if (plugin && plugin.onSubmit) { + plugin.onSubmit(obj); + } + }, + onReset: (obj) => { + const plugin = wrappedPlugin(); + if (plugin && plugin.onReset) { + plugin.onReset(obj); + } + }, + getSources: (obj) => { + const plugin = wrappedPlugin(); + if (plugin && plugin.getSources) { + return plugin.getSources(obj); + } else { + return Promise.resolve([]); + } + }, + data: (obj) => { + const plugin = wrappedPlugin(); + if (plugin && plugin.data) { + plugin.data(obj); + } + }, + }; +} + +function validateItems(items) { + // Validate the first item + if (items.length > 0) { + const item = items[0]; + const missingFields = []; + if (item.href == undefined) { + missingFields.push("href"); + } + if (!item.title == undefined) { + missingFields.push("title"); + } + if (!item.text == undefined) { + missingFields.push("text"); + } + + if (missingFields.length === 1) { + throw { + name: `Error: Search index is missing the ${missingFields[0]} field.`, + message: `The items being returned for this search do not include all the required fields. Please ensure that your index items include the ${missingFields[0]} field or use index-fields in your _quarto.yml file to specify the field names.`, + }; + } else if (missingFields.length > 1) { + const missingFieldList = missingFields + .map((field) => { + return `${field}`; + }) + .join(", "); + + throw { + name: `Error: Search index is missing the following fields: ${missingFieldList}.`, + message: `The items being returned for this search do not include all the required fields. Please ensure that your index items includes the following fields: ${missingFieldList}, or use index-fields in your _quarto.yml file to specify the field names.`, + }; + } + } +} + +let lastQuery = null; +function showCopyLink(query, options) { + const language = options.language; + lastQuery = query; + // Insert share icon + const inputSuffixEl = window.document.body.querySelector( + ".aa-Form .aa-InputWrapperSuffix" + ); + + if (inputSuffixEl) { + let copyButtonEl = window.document.body.querySelector( + ".aa-Form .aa-InputWrapperSuffix .aa-CopyButton" + ); + + if (copyButtonEl === null) { + copyButtonEl = window.document.createElement("button"); + copyButtonEl.setAttribute("class", "aa-CopyButton"); + copyButtonEl.setAttribute("type", "button"); + copyButtonEl.setAttribute("title", language["search-copy-link-title"]); + copyButtonEl.onmousedown = (e) => { + e.preventDefault(); + e.stopPropagation(); + }; + + const linkIcon = "bi-clipboard"; + const checkIcon = "bi-check2"; + + const shareIconEl = window.document.createElement("i"); + shareIconEl.setAttribute("class", `bi ${linkIcon}`); + copyButtonEl.appendChild(shareIconEl); + inputSuffixEl.prepend(copyButtonEl); + + const clipboard = new window.ClipboardJS(".aa-CopyButton", { + text: function (_trigger) { + const copyUrl = new URL(window.location); + copyUrl.searchParams.set(kQueryArg, lastQuery); + copyUrl.searchParams.set(kResultsArg, "1"); + return copyUrl.toString(); + }, + }); + clipboard.on("success", function (e) { + // Focus the input + + // button target + const button = e.trigger; + const icon = button.querySelector("i.bi"); + + // flash "checked" + icon.classList.add(checkIcon); + icon.classList.remove(linkIcon); + setTimeout(function () { + icon.classList.remove(checkIcon); + icon.classList.add(linkIcon); + }, 1000); + }); + } + + // If there is a query, show the link icon + if (copyButtonEl) { + if (lastQuery && options["copy-button"]) { + copyButtonEl.style.display = "flex"; + } else { + copyButtonEl.style.display = "none"; + } + } + } +} + +/* Search Index Handling */ +// create the index +var fuseIndex = undefined; +async function readSearchData() { + // Initialize the search index on demand + if (fuseIndex === undefined) { + // create fuse index + const options = { + keys: [ + { name: "title", weight: 20 }, + { name: "section", weight: 20 }, + { name: "text", weight: 10 }, + ], + ignoreLocation: true, + threshold: 0.1, + }; + const fuse = new window.Fuse([], options); + + // fetch the main search.json + const response = await fetch(offsetURL("search.json")); + if (response.status == 200) { + return response.json().then(function (searchDocs) { + searchDocs.forEach(function (searchDoc) { + fuse.add(searchDoc); + }); + fuseIndex = fuse; + return fuseIndex; + }); + } else { + return Promise.reject( + new Error( + "Unexpected status from search index request: " + response.status + ) + ); + } + } + return fuseIndex; +} + +function inputElement() { + return window.document.body.querySelector(".aa-Form .aa-Input"); +} + +function focusSearchInput() { + setTimeout(() => { + const inputEl = inputElement(); + if (inputEl) { + inputEl.focus(); + } + }, 50); +} + +/* Panels */ +const kItemTypeDoc = "document"; +const kItemTypeMore = "document-more"; +const kItemTypeItem = "document-item"; +const kItemTypeError = "error"; + +function renderItem( + item, + createElement, + state, + setActiveItemId, + setContext, + refresh +) { + switch (item.type) { + case kItemTypeDoc: + return createDocumentCard( + createElement, + "file-richtext", + item.title, + item.section, + item.text, + item.href + ); + case kItemTypeMore: + return createMoreCard( + createElement, + item, + state, + setActiveItemId, + setContext, + refresh + ); + case kItemTypeItem: + return createSectionCard( + createElement, + item.section, + item.text, + item.href + ); + case kItemTypeError: + return createErrorCard(createElement, item.title, item.text); + default: + return undefined; + } +} + +function createDocumentCard(createElement, icon, title, section, text, href) { + const iconEl = createElement("i", { + class: `bi bi-${icon} search-result-icon`, + }); + const titleEl = createElement("p", { class: "search-result-title" }, title); + const titleContainerEl = createElement( + "div", + { class: "search-result-title-container" }, + [iconEl, titleEl] + ); + + const textEls = []; + if (section) { + const sectionEl = createElement( + "p", + { class: "search-result-section" }, + section + ); + textEls.push(sectionEl); + } + const descEl = createElement("p", { + class: "search-result-text", + dangerouslySetInnerHTML: { + __html: text, + }, + }); + textEls.push(descEl); + + const textContainerEl = createElement( + "div", + { class: "search-result-text-container" }, + textEls + ); + + const containerEl = createElement( + "div", + { + class: "search-result-container", + }, + [titleContainerEl, textContainerEl] + ); + + const linkEl = createElement( + "a", + { + href: offsetURL(href), + class: "search-result-link", + }, + containerEl + ); + + const classes = ["search-result-doc", "search-item"]; + if (!section) { + classes.push("document-selectable"); + } + + return createElement( + "div", + { + class: classes.join(" "), + }, + linkEl + ); +} + +function createMoreCard( + createElement, + item, + state, + setActiveItemId, + setContext, + refresh +) { + const moreCardEl = createElement( + "div", + { + class: "search-result-more search-item", + onClick: (e) => { + // Handle expanding the sections by adding the expanded + // section to the list of expanded sections + toggleExpanded(item, state, setContext, setActiveItemId, refresh); + e.stopPropagation(); + }, + }, + item.title + ); + + return moreCardEl; +} + +function toggleExpanded(item, state, setContext, setActiveItemId, refresh) { + const expanded = state.context.expanded || []; + if (expanded.includes(item.target)) { + setContext({ + expanded: expanded.filter((target) => target !== item.target), + }); + } else { + setContext({ expanded: [...expanded, item.target] }); + } + + refresh(); + setActiveItemId(item.__autocomplete_id); +} + +function createSectionCard(createElement, section, text, href) { + const sectionEl = createSection(createElement, section, text, href); + return createElement( + "div", + { + class: "search-result-doc-section search-item", + }, + sectionEl + ); +} + +function createSection(createElement, title, text, href) { + const descEl = createElement("p", { + class: "search-result-text", + dangerouslySetInnerHTML: { + __html: text, + }, + }); + + const titleEl = createElement("p", { class: "search-result-section" }, title); + const linkEl = createElement( + "a", + { + href: offsetURL(href), + class: "search-result-link", + }, + [titleEl, descEl] + ); + return linkEl; +} + +function createErrorCard(createElement, title, text) { + const descEl = createElement("p", { + class: "search-error-text", + dangerouslySetInnerHTML: { + __html: text, + }, + }); + + const titleEl = createElement("p", { + class: "search-error-title", + dangerouslySetInnerHTML: { + __html: ` ${title}`, + }, + }); + const errorEl = createElement("div", { class: "search-error" }, [ + titleEl, + descEl, + ]); + return errorEl; +} + +function positionPanel(pos) { + const panelEl = window.document.querySelector( + "#quarto-search-results .aa-Panel" + ); + const inputEl = window.document.querySelector( + "#quarto-search .aa-Autocomplete" + ); + + if (panelEl && inputEl) { + panelEl.style.top = `${Math.round(panelEl.offsetTop)}px`; + if (pos === "start") { + panelEl.style.left = `${Math.round(inputEl.left)}px`; + } else { + panelEl.style.right = `${Math.round(inputEl.offsetRight)}px`; + } + } +} + +/* Highlighting */ +// highlighting functions +function highlightMatch(query, text) { + if (text) { + const start = text.toLowerCase().indexOf(query.toLowerCase()); + if (start !== -1) { + const startMark = ""; + const endMark = ""; + + const end = start + query.length; + text = + text.slice(0, start) + + startMark + + text.slice(start, end) + + endMark + + text.slice(end); + const startInfo = clipStart(text, start); + const endInfo = clipEnd( + text, + startInfo.position + startMark.length + endMark.length + ); + text = + startInfo.prefix + + text.slice(startInfo.position, endInfo.position) + + endInfo.suffix; + + return text; + } else { + return text; + } + } else { + return text; + } +} + +function clipStart(text, pos) { + const clipStart = pos - 50; + if (clipStart < 0) { + // This will just return the start of the string + return { + position: 0, + prefix: "", + }; + } else { + // We're clipping before the start of the string, walk backwards to the first space. + const spacePos = findSpace(text, pos, -1); + return { + position: spacePos.position, + prefix: "", + }; + } +} + +function clipEnd(text, pos) { + const clipEnd = pos + 200; + if (clipEnd > text.length) { + return { + position: text.length, + suffix: "", + }; + } else { + const spacePos = findSpace(text, clipEnd, 1); + return { + position: spacePos.position, + suffix: spacePos.clipped ? "…" : "", + }; + } +} + +function findSpace(text, start, step) { + let stepPos = start; + while (stepPos > -1 && stepPos < text.length) { + const char = text[stepPos]; + if (char === " " || char === "," || char === ":") { + return { + position: step === 1 ? stepPos : stepPos - step, + clipped: stepPos > 1 && stepPos < text.length, + }; + } + stepPos = stepPos + step; + } + + return { + position: stepPos - step, + clipped: false, + }; +} + +// removes highlighting as implemented by the mark tag +function clearHighlight(searchterm, el) { + const childNodes = el.childNodes; + for (let i = childNodes.length - 1; i >= 0; i--) { + const node = childNodes[i]; + if (node.nodeType === Node.ELEMENT_NODE) { + if ( + node.tagName === "MARK" && + node.innerText.toLowerCase() === searchterm.toLowerCase() + ) { + el.replaceChild(document.createTextNode(node.innerText), node); + } else { + clearHighlight(searchterm, node); + } + } + } +} + +// highlight matches +function highlight(term, el) { + const termRegex = new RegExp(term, "ig"); + const childNodes = el.childNodes; + + // walk back to front avoid mutating elements in front of us + for (let i = childNodes.length - 1; i >= 0; i--) { + const node = childNodes[i]; + + if (node.nodeType === Node.TEXT_NODE) { + // Search text nodes for text to highlight + const text = node.nodeValue; + + let startIndex = 0; + let matchIndex = text.search(termRegex); + if (matchIndex > -1) { + const markFragment = document.createDocumentFragment(); + while (matchIndex > -1) { + const prefix = text.slice(startIndex, matchIndex); + markFragment.appendChild(document.createTextNode(prefix)); + + const mark = document.createElement("mark"); + mark.appendChild( + document.createTextNode( + text.slice(matchIndex, matchIndex + term.length) + ) + ); + markFragment.appendChild(mark); + + startIndex = matchIndex + term.length; + matchIndex = text.slice(startIndex).search(new RegExp(term, "ig")); + if (matchIndex > -1) { + matchIndex = startIndex + matchIndex; + } + } + if (startIndex < text.length) { + markFragment.appendChild( + document.createTextNode(text.slice(startIndex, text.length)) + ); + } + + el.replaceChild(markFragment, node); + } + } else if (node.nodeType === Node.ELEMENT_NODE) { + // recurse through elements + highlight(term, node); + } + } +} + +/* Link Handling */ +// get the offset from this page for a given site root relative url +function offsetURL(url) { + var offset = getMeta("quarto:offset"); + return offset ? offset + url : url; +} + +// read a meta tag value +function getMeta(metaName) { + var metas = window.document.getElementsByTagName("meta"); + for (let i = 0; i < metas.length; i++) { + if (metas[i].getAttribute("name") === metaName) { + return metas[i].getAttribute("content"); + } + } + return ""; +} + +function algoliaSearch(query, limit, algoliaOptions) { + const { getAlgoliaResults } = window["@algolia/autocomplete-preset-algolia"]; + + const applicationId = algoliaOptions["application-id"]; + const searchOnlyApiKey = algoliaOptions["search-only-api-key"]; + const indexName = algoliaOptions["index-name"]; + const indexFields = algoliaOptions["index-fields"]; + const searchClient = window.algoliasearch(applicationId, searchOnlyApiKey); + const searchParams = algoliaOptions["params"]; + const searchAnalytics = !!algoliaOptions["analytics-events"]; + + return getAlgoliaResults({ + searchClient, + queries: [ + { + indexName: indexName, + query, + params: { + hitsPerPage: limit, + clickAnalytics: searchAnalytics, + ...searchParams, + }, + }, + ], + transformResponse: (response) => { + if (!indexFields) { + return response.hits.map((hit) => { + return hit.map((item) => { + return { + ...item, + text: highlightMatch(query, item.text), + }; + }); + }); + } else { + const remappedHits = response.hits.map((hit) => { + return hit.map((item) => { + const newItem = { ...item }; + ["href", "section", "title", "text"].forEach((keyName) => { + const mappedName = indexFields[keyName]; + if ( + mappedName && + item[mappedName] !== undefined && + mappedName !== keyName + ) { + newItem[keyName] = item[mappedName]; + delete newItem[mappedName]; + } + }); + newItem.text = highlightMatch(query, newItem.text); + return newItem; + }); + }); + return remappedHits; + } + }, + }); +} + +function fuseSearch(query, fuse, fuseOptions) { + return fuse.search(query, fuseOptions).map((result) => { + const addParam = (url, name, value) => { + const anchorParts = url.split("#"); + const baseUrl = anchorParts[0]; + const sep = baseUrl.search("\\?") > 0 ? "&" : "?"; + anchorParts[0] = baseUrl + sep + name + "=" + value; + return anchorParts.join("#"); + }; + + return { + title: result.item.title, + section: result.item.section, + href: addParam(result.item.href, kQueryArg, query), + text: highlightMatch(query, result.item.text), + }; + }); +} diff --git a/doc/_quarto.yml b/doc/_quarto.yml new file mode 100644 index 0000000..630ec63 --- /dev/null +++ b/doc/_quarto.yml @@ -0,0 +1,25 @@ +project: + type: book + +book: + title: "OBITools V4" + author: "Eric Coissac" + date: "1/17/2023" + chapters: + - index.qmd + - intro.qmd + - commands.qmd + - library.qmd + - annexes.qmd + - references.qmd + +bibliography: book.bib + +format: + html: + theme: cosmo + pdf: + documentclass: scrreprt + + + diff --git a/doc/annexes.qmd b/doc/annexes.qmd new file mode 100644 index 0000000..80d7e27 --- /dev/null +++ b/doc/annexes.qmd @@ -0,0 +1,82 @@ +# Annexes + +### Sequence attributes + +#### Reserved sequence attributes + +##### `ali_dir` + +###### Type : `string` + +The attribute can contain 2 string values `"left"` or `"right".` + +###### Set by the *obipairing* tool + +The alignment generated by *obipairing* is a 3'-end gap free algorithm. +Two cases can occur when aligning the forward and reverse reads. If the +barcode is long enough, both the reads overlap only on their 3' ends. In +such case, the alignment direction `ali_dir` is set to *left*. If the +barcode is shorter than the read length, the paired reads overlap by +their 5' ends, and the complete barcode is sequenced by both the reads. +In that later case, `ali_dir` is set to *right*. + +##### `ali_length` + +###### Set by the *obipairing* tool + +Length of the aligned parts when merging forward and reverse reads + +##### `count` : the number of sequence occurrences + +###### Set by the *obiuniq* tool + +The `count` attribute indicates how-many strictly identical sequences +have been merged in a single record. It contains an integer value. If it +is absent this means that the sequence record represents a single +occurrence of the sequence. + +###### Getter : method `Count()` + +The `Count()` method allows to access to the count attribute as an +integer value. If the `count` attribute is not defined for the given +sequence, the value *1* is returned + +##### `merged_*` + +###### Type : `map[string]int` + +###### Set by the *obiuniq* tool + +The `-m` option of the *obiuniq* tools allows for keeping track of the +distribution of the values stored in given attribute of interest. Often +this option is used to summarise distribution of a sequence variant +accross samples when *obiuniq* is run after running *obimultiplex*. The +actual name of the attribute depends on the name of the monitored +attribute. If `-m` option is used with the attribute *sample*, then this +attribute names *merged_sample*. + +##### `mode` + +###### Set by the *obipairing* tool + +**`obitag_ref_index`** + +###### Set by the *obirefidx* tool. + +It resumes to which taxonomic annotation a match to that sequence must +lead according to the number of differences existing between the query +sequence and the reference sequence having that tag. + +###### Getter : method `Count()` + +##### `pairing_mismatches` + +###### Set by the *obipairing* tool + +##### `score` + +###### Set by the *obipairing* tool + +##### `score_norm` + +###### Set by the *obipairing* tool diff --git a/doc/book.bib b/doc/book.bib index e69de29..1df4f9c 100644 --- a/doc/book.bib +++ b/doc/book.bib @@ -0,0 +1,10 @@ +@article{cock2010sanger, + title={The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants}, + author={Cock, Peter JA and Fields, Christopher J and Goto, Naohisa and Heuer, Michael L and Rice, Peter M}, + journal={Nucleic acids research}, + volume={38}, + number={6}, + pages={1767--1771}, + year={2010}, + publisher={Oxford University Press} +} diff --git a/doc/commands.qmd b/doc/commands.qmd new file mode 100644 index 0000000..b5f474a --- /dev/null +++ b/doc/commands.qmd @@ -0,0 +1,121 @@ +# The *OBITools* commands + +## Specifying the input files to *OBITools* commands + +## Options common to most of the *OBITools* commands + +### Specifying input format + +Five sequence formats are accepted for input files. [Fasta](#fasta-classical "Fasta format description") and [Fastq](#fastq-classical "Fastq format description") are the main ones, EMBL and Genbank allow the use of flat files produced by these two international databases. The last one, ecoPCR, is maintained for compatibility with previous *OBITools* and allows to read *ecoPCR* outputs as sequence files. + +- `--ecopcr` : Read data following the *ecoPCR* output format. +- `--embl` Read data following the *EMBL* flatfile format. +- `--genbank` Read data following the *Genbank* flatfile format. + +Several encoding schemes have been proposed for quality scores in [Fastq](#fastq-classical "Fastq format description") format. Currently, *OBITools* considers Sanger encoding as the standard. For reasons of compatibility with older datasets produced with *Solexa* sequencers, it is possible, by using the following option, to force the use of the corresponding quality encoding scheme when reading these older files. + +- `--solexa` Decodes quality string according to the Solexa specification. (default: false) + +### Specifying output format + +Only two output sequence formats are supported by OBITools, Fasta and Fastq. Fastq is used when output sequences are associated with quality information. Otherwise, Fasta is the default format. However, it is possible to force the output format by using one of the following two options. Forcing the use of Fasta results in the loss of quality information. Conversely, when the Fastq format is forced with sequences that have no quality data, dummy qualities set to 40 for each nucleotide are added. + +- `--fasta-output` Read data following the ecoPCR output format. +- `--fastq-output` Read data following the EMBL flatfile format. + +OBITools allows multiple input files to be specified for a single command. + +- `--no-order` When several input files are provided, indicates that there is no order among them. (default: false) + +### Format of the annotations in Fasta and Fastq files + +OBITools extend the [Fasta](#fasta-classical "Fasta format description") and [Fastq](#fastq-classical "Fastq format description") formats by introducing a format for the title lines of these formats allowing to annotate every sequence. While the previous version of OBITools used an *ad-hoc* format for these annotation, this new version introduce the usage of the standard JSON format to store them. + +On input, OBITools automatically recognize the format of the annotations, but two options allows to force the parsing following one of them. You should normally not need to use these options. + +- `--input-OBI-header` FASTA/FASTQ title line annotations follow OBI format. (default: false) + +- `--input-json-header` FASTA/FASTQ title line annotations follow json format. (default: false) + +On output, by default annotation are formatted using the new JSON format. For compatibility with previous version of OBITools and with external scripts and software, it is possible to force the usage of the previous OBITools format. + +- `--output-OBI-header|-O` output FASTA/FASTQ title line annotations follow OBI format. (default: false) + +- `--output-json-header` output FASTA/FASTQ title line annotations follow json format. (default: false) + +#### System related options + +- `--debug` (default: false) +- `--help\|-h\|-?` (default: false) +- `--max-cpu ` Number of parallele threads computing the result (default: 10) +- `--workers\|-w ` Number of parallele threads computing the result (default: 9) + +## OBITools expression language + +Several OBITools (*e.g.* obigrep, obiannotate) allow the user to specify some simple expressions to compute values or define predicates. This expressions are parsed and evaluated using the [gval](https://pkg.go.dev/github.com/PaesslerAG/gval "Gval (Go eVALuate) for evaluating arbitrary expressions Go-like expressions.") go package, which allows for evaluating go-Like expression. + +### Variables usable in the expression + +#### sequence + +sequence is the sequence object on which the expression is evaluated + +#### annotation + +### Function defined in the language + +#### len + +#### ismap + +#### hasattribute + +#### min + +#### max + +### Accessing to the sequence annotations + +## Metabarcode design and quality assessment + +#### `obipcr` + +> Replace the `ecoPCR` original *OBITools* + +## File format conversions + +#### `obiconvert` + +## Sequence annotations + +#### `obitag` + +## Computations on sequences + +### `obipairing` + +> Replace the `illuminapairedends` original *OBITools* + +#### `obimultiplex` + +> Replace the `ngsfilter` original *OBITools* + +#### `obicomplement` + +#### `obiclean` + +#### `obiuniq` + +## Sequence sampling and filtering + +#### `obigrep` + +### Utilities + +#### `obicount` + +#### `obidistribute` + +#### `obifind` + +> Replace the `ecofind` original *OBITools.* diff --git a/doc/cover.png b/doc/cover.png new file mode 100644 index 0000000000000000000000000000000000000000..e1f5bc61d11049afe5b5dc2a67a3a32f406be964 GIT binary patch literal 51194 zcmeGEc|4SD-v^E}jk1(1(TY*FlxR`eGHD^Dh?$s5v}zCf)yz1~&-pn%`*s{Voafs2Qyiiw zBO}vqmc#V2X?wuKFvlE0Xa9b;zN0~?Q8lV2_81+HF!Hxu|%##&P=|Kqo= z_gI^fm;Zr`bf?s>*y$C)YI&h3O zQB!`6&C#FUrzd7dANr*$J5_0!c>kKj)m8__F%2@NEgbgi(9wxG`h&l4p6}m!^uAuN zLGzOj^*z~7H$`))$NnGVRcI6RA2BOl&9gpuiD7%M+KZtwEAspLs#RL=^TI!0t^1Hy z&I!+GZug0~`u$kecTPc7-R0o2A7QEC3r2OSFqly?vNXE<|9$vBBK)5u{GS&5f2sw% zFu5nqhfa^{LPGq7mQ=#+Xes*iqvr4!t%9&D&yF3$|qh{MYCEceD+)UDf>J%geLfGF}!FCFaLj zoZBz8enyPOcUA2*sp~shR{ct8u76i4;eLHzxY;N#>}kuR*v|G5<1cSsE1w-1kyH7z zzBaUZRFb4t$8T*xhhfzh{eq^X@#VpmU3N5Wwk(fzc$s8?^n1W|@$=2!G(s|`gngUQ 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This was at the beginning of the DNA metabarcoding story at the Laboratoire d'Ecologie Alpine (LECA) in Grenoble. At that time, with Pierre Taberlet and François Pompanon, we were thinking about the potential of this new methodology under development. PIerre and François developed more the laboratory methods, while I was thinking more about the tools for analysing the sequences produced. Two ideas were behind this development. I wanted something modular, and something easy to extend. To achieve the first goal, I decided to implement obitools as a suite of unix commands mimicking the classic unix commands but dedicated to sequence files. The basic unix commands are very useful for automatically manipulating, parsing and editing text files. They work in flow, line by line on the input text. The result is a new text file that can be used as input for the next command. Such a design makes it possible to quickly develop a text processing pipeline by chaining simple elementary operations. The *OBITools* are the exact counterpart of these basic Unix commands, but the basic information they process is a sequence (potentially spanning several lines of text), not a single line of text. Most *OBITools* consume sequence files and produce sequence files. Thus, the principles of chaining and modularity are respected. In order to be able to easily extend the *OBITools* to keep up with our evolving ideas about processing DNA metabarcoding data, it was decided to develop them using an interpreted language: Python. Python 2, the version available at the time, allowed us to develop the *OBITools* efficiently. When parts of the algorithms were computationally demanding, they were implemented in C and linked to the Python code. Even though Python is not the most efficient language available, even though computers were not as powerful as they are today, the size of the data we could produce using 454 sequencers or early solexa machines was small enough to be processed in a reasonable time. + + diff --git a/doc/intro.qmd b/doc/intro.qmd new file mode 100644 index 0000000..4b5845d --- /dev/null +++ b/doc/intro.qmd @@ -0,0 +1,142 @@ +# The OBITools + +## Aims of *OBITools* + +## File formats usable with *OBITools* + +### The sequence files + +Sequences can be stored following various format. OBITools knows some of them. The central formats for sequence files manipulated by OBITools scripts are the `fasta` and fastq format. OBITools extends the both these formats by specifying a syntax to include in the definition line data qualifying the sequence. All file formats use the `IUPAC` code for encoding nucleotides. + +### The IUPAC Code + +The International Union of Pure and Applied Chemistry (IUPAC\_) defined the standard code for representing protein or DNA sequences. + +#### Nucleic IUPAC Code {#DNA-IUPAC} + +| **Code** | **Nucleotide** | +|----------|-----------------------------| +| A | Adenine | +| C | Cytosine | +| G | Guanine | +| T | Thymine | +| U | Uracil | +| R | Purine (A or G) | +| Y | Pyrimidine (C, T, or U) | +| M | C or A | +| K | T, U, or G | +| W | T, U, or A | +| S | C or G | +| B | C, T, U, or G (not A) | +| D | A, T, U, or G (not C) | +| H | A, T, U, or C (not G) | +| V | A, C, or G (not T, not U) | +| N | Any base (A, C, G, T, or U) | + +### The *fasta* format {#classical-fasta} + +The **fasta format** is certainly the most widely used sequence file format. This is certainly due to its great simplicity. It was originally created for the Lipman and Pearson [FASTA program](http://www.ncbi.nlm.nih.gov/pubmed/3162770?dopt=Citation). OBITools use in more of the classical :ref:`fasta` format an :ref:`extended version` of this format where structured data are included in the title line. + +In *fasta* format a sequence is represented by a title line beginning with a **`>`** character and the sequences by itself following the :doc:`iupac` code. The sequence is usually split other severals lines of the same length (expect for the last one) + + >my_sequence this is my pretty sequence + ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT + GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT + AACGACGTTGCAGTACGTTGCAGT + +This is no special format for the title line excepting that this line should be unique. Usually the first word following the **\>** character is considered as the sequence identifier. The end of the title line corresponding to a description of the sequence. Several sequences can be concatenated in a same file. The description of the next sequence is just pasted at the end of the record of the previous one + + >sequence_A this is my first pretty sequence + ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT + GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT + AACGACGTTGCAGTACGTTGCAGT + >sequence_B this is my second pretty sequence + ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT + GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT + AACGACGTTGCAGTACGTTGCAGT + >sequence_C this is my third pretty sequence + ACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGT + GTGCTGACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTACGTTGCAGTGTTT + AACGACGTTGCAGTACGTTGCAGT + +### The *fastq* sequence format[^01_obitools_doc-1] {#classical-fastq} + +**fastq format** is a text-based format for storing both a biological sequence (usually nucleotide sequence) and its corresponding quality scores. Both the sequence letter and quality score are encoded with a single ASCII character for brevity. It was originally developed at the `Wellcome Trust Sanger Institute` to bundle a [fasta](#classical-fasta) sequence and its quality data, but has recently become the *de facto* standard for storing the output of high throughput sequencing instruments such as the Illumina Genome Analyzer Illumina [@cock2010sanger] . + +[^01_obitools_doc-1]: This article uses material from the Wikipedia article [`FASTQ format`](http://en.wikipedia.org/wiki/FASTQ_format) which is released under the `Creative Commons Attribution-Share-Alike License 3.0` + +A fastq file normally uses four lines per sequence. + +- Line 1 begins with a '\@' character and is followed by a sequence identifier and an *optional* description (like a :ref:`fasta` title line). +- Line 2 is the raw sequence letters. +- Line 3 begins with a '+' character and is *optionally* followed by the same sequence identifier (and any description) again. +- Line 4 encodes the quality values for the sequence in Line 2, and must contain the same number of symbols as letters in the sequence. + +A fastq file containing a single sequence might look like this: + + @SEQ_ID + GATTTGGGGTTCAAAGCAGTATCGATCAAATAGTAAATCCATTTGTTCAACTCACAGTTT + + + !''*((((***+))%%%++)(%%%%).1***-+*''))**55CCF>>>>>>CCCCCCC65 + +The character '!' represents the lowest quality while '\~' is the highest. Here are the quality value characters in left-to-right increasing order of quality (`ASCII`): + + !"#$%&'()*+,-./0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ[\]^_`abcdefghijklmnopqrstuvwxyz{|}~ + +The original Sanger FASTQ files also allowed the sequence and quality strings to be wrapped (split over multiple lines), but this is generally discouraged as it can make parsing complicated due to the unfortunate choice of "\@" and "+" as markers (these characters can also occur in the quality string). + +#### Variations + +##### Quality + +A quality value *Q* is an integer mapping of *p* (i.e., the probability that the corresponding base call is incorrect). Two different equations have been in use. The first is the standard Sanger variant to assess reliability of a base call, otherwise known as Phred quality score: + +$$ +Q_\text{sanger} = -10 \, \log_{10} p +$$ + +The Solexa pipeline (i.e., the software delivered with the Illumina Genome Analyzer) earlier used a different mapping, encoding the odds $\mathbf{p}/(1-\mathbf{p})$ instead of the probability $\mathbf{p}$: + +$$ +Q_\text{solexa-prior to v.1.3} = -10 \, \log_{10} \frac{p}{1-p} +$$ + +Although both mappings are asymptotically identical at higher quality values, they differ at lower quality levels (i.e., approximately $\mathbf{p} > 0.05$, or equivalently, $\mathbf{Q} < 13$). + +\|Relationship between *Q* and *p* using the Sanger (red) and Solexa (black) equations (described above). The vertical dotted line indicates $\mathbf{p}= 0.05$, or equivalently, $Q = 13$.\| + +#### Encoding + +- Sanger format can encode a Phred quality score from 0 to 93 using ASCII 33 to 126 (although in raw read data the Phred quality score rarely exceeds 60, higher scores are possible in assemblies or read maps). +- Solexa/Illumina 1.0 format can encode a Solexa/Illumina quality score from -5 to 62 using ASCII 59 to 126 (although in raw read data Solexa scores from -5 to 40 only are expected) +- Starting with Illumina 1.3 and before Illumina 1.8, the format encoded a Phred quality score from 0 to 62 using ASCII 64 to 126 (although in raw read data Phred scores from 0 to 40 only are expected). +- Starting in Illumina 1.5 and before Illumina 1.8, the Phred scores 0 to 2 have a slightly different meaning. The values 0 and 1 are no longer used and the value 2, encoded by ASCII 66 "B". + +Sequencing Control Software, Version 2.6, Catalog \# SY-960-2601, Part \# 15009921 Rev. A, November 2009] [[http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf\\\\](http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf){.uri}](%5Bhttp://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf\%5D(http://watson.nci.nih.gov/solexa/Using_SCSv2.6_15009921_A.pdf)%7B.uri%7D){.uri} (page 30) states the following: *If a read ends with a segment of mostly low quality (Q15 or below), then all of the quality values in the segment are replaced with a value of 2 (encoded as the letter B in Illumina's text-based encoding of quality scores)... This Q2 indicator does not predict a specific error rate, but rather indicates that a specific final portion of the read should not be used in further analyses.* Also, the quality score encoded as "B" letter may occur internally within reads at least as late as pipeline version 1.6, as shown in the following example: + + @HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1 + TTAATTGGTAAATAAATCTCCTAATAGCTTAGATNTTACCTTNNNNNNNNNNTAGTTTCTTGAGATTTGTTGGGGGAGACATTTTTGTGATTGCCTTGAT + +HWI-EAS209_0006_FC706VJ:5:58:5894:21141#ATCACG/1 + efcfffffcfeefffcffffffddf`feed]`]_Ba_^__[YBBBBBBBBBBRTT\]][]dddd`ddd^dddadd^BBBBBBBBBBBBBBBBBBBBBBBB + +An alternative interpretation of this ASCII encoding has been proposed. Also, in Illumina runs using PhiX controls, the character 'B' was observed to represent an "unknown quality score". The error rate of 'B' reads was roughly 3 phred scores lower the mean observed score of a given run. + +- Starting in Illumina 1.8, the quality scores have basically returned to the use of the Sanger format (Phred+33). + +## File extension + +There is no standard file extension for a FASTQ file, but .fq and .fastq, are commonly used. + +## See also + +- :ref:`fasta` + +## References + +.. [1] Cock et al (2009) The Sanger FASTQ file format for sequences with quality scores, and the Solexa/Illumina FASTQ variants. Nucleic Acids Research, + +.. [2] Illumina Quality Scores, Tobias Mann, Bioinformatics, San Diego, Illumina `1`\_\_ + +.. \|Relationship between *Q* and *p* using the Sanger (red) and Solexa (black) equations (described above). The vertical dotted line indicates *p* = 0.05, or equivalently, *Q* Å 13.\| image:: Probability metrics.png + +See diff --git a/doc/library.qmd b/doc/library.qmd new file mode 100644 index 0000000..ccfa9e0 --- /dev/null +++ b/doc/library.qmd @@ -0,0 +1,157 @@ +# The GO *OBITools* library + +## BioSequence + +The `BioSequence` class is used to represent biological sequences. It +allows for storing : - the sequence itself as a `[]byte` - the +sequencing quality score as a `[]byte` if needed - an identifier as a +`string` - a definition as a `string` - a set of *(key, value)* pairs in +a `map[sting]interface{}` + +BioSequence is defined in the obiseq module and is included using the +code + +``` go +import ( + "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq" +) +``` + +### Creating new instances + +To create new instance, use + +- `MakeBioSequence(id string, sequence []byte, definition string) obiseq.BioSequence` +- `NewBioSequence(id string, sequence []byte, definition string) *obiseq.BioSequence` + +Both create a `BioSequence` instance, but when the first one returns the +instance, the second returns a pointer on the new instance. Two other +functions `MakeEmptyBioSequence`, and `NewEmptyBioSequence` do the same +job but provide an uninitialized objects. + +- `id` parameters corresponds to the unique identifier of the + sequence. It mist be a string constituted of a single word (not + containing any space). +- `sequence` is the DNA sequence itself, provided as a `byte` array + (`[]byte`). +- `definition` is a `string`, potentially empty, but usualy containing + a sentence explaining what is that sequence. + +``` go +import ( + "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq" +) + +func main() { + myseq := obiseq.NewBiosequence( + "seq_GH0001", + bytes.FromString("ACGTGTCAGTCG"), + "A short test sequence", + ) +} +``` + +When formated as fasta the parameters correspond to the following schema + + >id definition containing potentially several words + sequence + +### End of life of a `BioSequence` instance + +When a `BioSequence` instance is no more used, it is normally taken in +charge by the GO garbage collector. You can if you want call the +`Recycle` method on the instance to store the allocated memory element +in a `pool` to limit allocation effort when many sequences are +manipulated. + +### Accessing to the elements of a sequence + +The different elements of an `obiseq.BioSequence` must be accessed using +a set of methods. For the three main elements provided during the +creation of a new instance methodes are : + +- `Id() string` +- `Sequence() []byte` +- `Definition() string` + +It exists pending method to change the value of these elements + +- `SetId(id string)` +- `SetSequence(sequence []byte)` +- `SetDefinition(definition string)` + +``` go +import ( + "fmt" + "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq" +) + +func main() { + myseq := obiseq.NewBiosequence( + "seq_GH0001", + bytes.FromString("ACGTGTCAGTCG"), + "A short test sequence", + ) + + fmt.Println(myseq.Id()) + myseq.SetId("SPE01_0001") + fmt.Println(myseq.Id()) +} +``` + +#### Different ways for accessing an editing the sequence + +If `Sequence()`and `SetSequence(sequence []byte)` methods are the basic +ones, several other methods exist. + +- `String() string` return the sequence directly converted to a + `string` instance. +- The `Write` method family allows for extending an existing sequence + following the buffer protocol. + - `Write(data []byte) (int, error)` allows for appending a byte + array on 3' end of the sequence. + - `WriteString(data string) (int, error)` allows for appending a + `string`. + - `WriteByte(data byte) error` allows for appending a single + `byte`. + +The `Clear` method empties the sequence buffer. + +``` go +import ( + "fmt" + "git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq" +) + +func main() { + myseq := obiseq.NewEmptyBiosequence() + + myseq.WriteString("accc") + myseq.WriteByte(byte('c')) + fmt.Println(myseq.String()) +} +``` + +#### Sequence quality scores + +Sequence quality scores cannot be initialized at the time of instance +creation. You must use dedicated methods to add quality scores to a +sequence. + +To be coherent the length of both the DNA sequence and que quality score +sequence must be equal. But assessment of this constraint is realized. +It is of the programmer responsability to check that invariant. + +While accessing to the quality scores relies on the method +`Quality() []byte`, setting the quality need to call one of the +following method. They run similarly to their sequence dedicated +conterpart. + +- `SetQualities(qualities Quality)` +- `WriteQualities(data []byte) (int, error)` +- `WriteByteQualities(data byte) error` + +In a way analogous to the `Clear` method, `ClearQualities()` empties the +sequence of quality scores. + + diff --git a/doc/references.qmd b/doc/references.qmd new file mode 100644 index 0000000..925f7c4 --- /dev/null +++ b/doc/references.qmd @@ -0,0 +1,4 @@ +# References {.unnumbered} + +::: {#refs} +::: diff --git a/doc/summary.qmd b/doc/summary.qmd new file mode 100644 index 0000000..b450ab7 --- /dev/null +++ b/doc/summary.qmd @@ -0,0 +1,3 @@ +# Summary + +In summary, this book has no content whatsoever.