diff --git a/pkg/obiformats/fastaseq_read.go b/pkg/obiformats/fastaseq_read.go
index c902857..a61414c 100644
--- a/pkg/obiformats/fastaseq_read.go
+++ b/pkg/obiformats/fastaseq_read.go
@@ -16,7 +16,7 @@ import (
 	log "github.com/sirupsen/logrus"
 )
 
-// lastSequenceCut extracts the up to the last sequence cut from a given buffer.
+// lastFastaCut extracts the up to the last sequence cut from a given buffer.
 //
 // It takes a parameter:
 //   - buffer []byte: the buffer to extract the sequence cut from.
@@ -24,7 +24,7 @@ import (
 // It returns two values:
 //   - []byte: the extracted sequences.
 //   - []byte: the remaining buffer after the sequence cut (the last sequence).
-func lastSequenceCut(buffer []byte) ([]byte, []byte) {
+func lastFastaCut(buffer []byte) ([]byte, []byte) {
 	imax := len(buffer)
 	last := 0
 	state := 0
@@ -45,13 +45,13 @@ func lastSequenceCut(buffer []byte) ([]byte, []byte) {
 	return []byte{}, buffer
 }
 
-// firstSequenceCut cuts the input buffer at the first occurrence of a ">" character
+// firstFastaCut cuts the input buffer at the first occurrence of a ">" character
 // following a sequence of "\r" or "\n" characters.
 //
 // It takes a byte slice as input, representing the buffer to be cut.
 // It returns two byte slices: the first slice contains the part of the buffer before the cut,
 // and the second slice contains the part of the buffer after the cut.
-func firstSequenceCut(buffer []byte) ([]byte, []byte) {
+func firstFastaCut(buffer []byte) ([]byte, []byte) {
 	imax := len(buffer)
 	last := 0
 	state := 0
@@ -73,17 +73,6 @@ func firstSequenceCut(buffer []byte) ([]byte, []byte) {
 
 }
 
-func fullSequenceCut(buffer []byte) ([]byte, []byte, []byte) {
-	before, buffer := firstSequenceCut(buffer)
-
-	if len(buffer) == 0 {
-		return before, []byte{}, []byte{}
-	}
-
-	buffer, after := lastSequenceCut(buffer)
-	return before, buffer, after
-}
-
 func Concatenate[S ~[]E, E any](s1, s2 S) S {
 	if len(s1) > 0 {
 		if len(s2) > 0 {
@@ -109,7 +98,7 @@ func FastaChunkReader(r io.Reader, size int, cutHead bool) (chan FastxChunk, err
 			buff = buff[:n]
 		}
 
-		begin, buff := firstSequenceCut(buff)
+		begin, buff := firstFastaCut(buff)
 
 		if len(begin) > 0 && !cutHead {
 			return out, fmt.Errorf("begin is not empty : %s", string(begin))
@@ -127,7 +116,7 @@ func FastaChunkReader(r io.Reader, size int, cutHead bool) (chan FastxChunk, err
 				buff = Concatenate(end, buff)
 				// fmt.Println("------------buff--pasted----------------")
 				// fmt.Println(string(buff))
-				buff, end = lastSequenceCut(buff)
+				buff, end = lastFastaCut(buff)
 				// fmt.Println("----------------buff--cutted------------")
 				// fmt.Println(string(buff))
 				// fmt.Println("------------------end-------------------")
diff --git a/pkg/obiformats/fastqseq_read.go b/pkg/obiformats/fastqseq_read.go
new file mode 100644
index 0000000..62b559d
--- /dev/null
+++ b/pkg/obiformats/fastqseq_read.go
@@ -0,0 +1,359 @@
+package obiformats
+
+import (
+	"bytes"
+	"io"
+	"os"
+	"path"
+
+	"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiiter"
+	"git.metabarcoding.org/lecasofts/go/obitools/pkg/obioptions"
+	"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
+	"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiutils"
+	log "github.com/sirupsen/logrus"
+	"golang.org/x/exp/slices"
+)
+
+func lastFastqCut(buffer []byte) ([]byte, []byte) {
+	imax := len(buffer)
+	cut := imax
+	state := 0
+	restart := imax - 1
+	for i := restart; i >= 0 && state < 7; i-- {
+		C := buffer[i]
+		is_end_of_line := C == '\r' || C == '\n'
+		is_space := C == ' ' || C == '\t'
+		is_sep := is_space || is_end_of_line
+
+		switch state {
+		case 0:
+			if C == '+' {
+				// Potential start of quality part step 1
+				state = 1
+				restart = i
+			}
+		case 1:
+			if is_end_of_line {
+				// Potential start of quality part step 2
+				state = 2
+			} else {
+				// it was not the start of quality part
+				state = 0
+				i = restart
+			}
+		case 2:
+			if is_sep {
+				// Potential start of quality part step 2 (stay in the same state)
+				state = 2
+			} else if (C >= 'a' && C <= 'z') || C == '-' || C == '.' {
+				// End of the sequence
+				state = 3
+			} else {
+				// it was not the start of quality part
+				state = 0
+				i = restart
+			}
+		case 3:
+			if is_end_of_line {
+				// Entrering in the header line
+				state = 4
+			} else if (C >= 'a' && C <= 'z') || C == '-' || C == '.' {
+				// progressing along of the sequence
+				state = 3
+			} else {
+				// it was not the sequence part
+				state = 0
+				i = restart
+			}
+		case 4:
+			if is_end_of_line {
+				state = 4
+			} else {
+				state = 5
+			}
+		case 5:
+			if is_end_of_line {
+				// It was not the header line
+				state = 0
+				i = restart
+			} else if C == '@' {
+				state = 6
+				cut = i
+			}
+		case 6:
+			if is_end_of_line {
+				state = 7
+			} else {
+				state = 0
+				i = restart
+			}
+		}
+	}
+	if state == 7 {
+		return buffer[:cut], bytes.Clone(buffer[cut:])
+	}
+	return []byte{}, buffer
+}
+
+func FastqChunkReader(r io.Reader, size int) (chan FastxChunk, error) {
+	out := make(chan FastxChunk)
+	buff := make([]byte, size)
+
+	n, err := r.Read(buff)
+
+	if n > 0 && err == nil {
+		if n < size {
+			buff = buff[:n]
+		}
+
+		go func(buff []byte) {
+			idx := 0
+			end := []byte{}
+
+			for err == nil && n > 0 {
+				// fmt.Println("============end=========================")
+				// fmt.Println(string(end))
+				// fmt.Println("------------buff------------------------")
+				// fmt.Println(string(buff))
+				buff = Concatenate(end, buff)
+				// fmt.Println("------------buff--pasted----------------")
+				// fmt.Println(string(buff))
+				buff, end = lastFastqCut(buff)
+				// fmt.Println("----------------buff--cutted------------")
+				// fmt.Println(string(buff))
+				// fmt.Println("------------------end-------------------")
+				// fmt.Println(string(end))
+				// fmt.Println("========================================")
+				if len(buff) > 0 {
+					out <- FastxChunk{
+						Bytes: bytes.Clone(buff),
+						index: idx,
+					}
+					idx++
+				}
+
+				buff = slices.Grow(buff[:0], size)[0:size]
+				n, err = r.Read(buff)
+				if n < size {
+					buff = buff[:n]
+				}
+				// fmt.Printf("n = %d, err = %v\n", n, err)
+			}
+
+			if len(end) > 0 {
+				out <- FastxChunk{
+					Bytes: bytes.Clone(end),
+					index: idx,
+				}
+			}
+
+			close(out)
+		}(buff)
+	}
+
+	return out, nil
+}
+
+func ParseFastqChunk(source string, ch FastxChunk, quality_shift byte) *obiiter.BioSequenceBatch {
+	slice := make(obiseq.BioSequenceSlice, 0, obioptions.CLIBatchSize())
+
+	state := 0
+	start := 0
+	current := 0
+	var identifier string
+	var definition string
+
+	for i := 0; i < len(ch.Bytes); i++ {
+		C := ch.Bytes[i]
+		is_end_of_line := C == '\r' || C == '\n'
+		is_space := C == ' ' || C == '\t'
+		is_sep := is_space || is_end_of_line
+
+		switch state {
+		case 0:
+			if C == '@' {
+				// Beginning of sequence
+				state = 1
+			}
+		case 1:
+			if is_sep {
+				// No identifier -> ERROR
+				log.Errorf("%s : sequence identifier is empty", source)
+				return nil
+			} else {
+				// Beginning of identifier
+				state = 2
+				start = i
+			}
+		case 2:
+			if is_sep {
+				// End of identifier
+				identifier = string(ch.Bytes[start:i])
+				state = 3
+			}
+		case 3:
+			if is_end_of_line {
+				// Definition empty
+				definition = ""
+				state = 5
+			} else if !is_space {
+				// Beginning of definition
+				start = i
+				state = 4
+			}
+		case 4:
+			if is_end_of_line {
+				definition = string(ch.Bytes[start:i])
+				state = 5
+
+			}
+		case 5:
+			if !is_end_of_line {
+				// Beginning of sequence
+				start = i
+				if C >= 'A' && C <= 'Z' {
+					ch.Bytes[current] = C + 'a' - 'A'
+				}
+				current = i + 1
+				state = 6
+			}
+		case 6:
+			if is_end_of_line {
+				// End of sequence
+				s := obiseq.NewBioSequence(identifier, bytes.Clone(ch.Bytes[start:current]), definition)
+				s.SetSource(source)
+				slice = append(slice, s)
+				state = 7
+			} else {
+				if C >= 'A' && C <= 'Z' {
+					ch.Bytes[current] = C + 'a' - 'A'
+				}
+				current = i + 1
+			}
+		case 7:
+			if is_end_of_line {
+				state = 7
+			} else if C == '+' {
+				state = 8
+			} else {
+				log.Errorf("%s[%s] : sequence data not followed by a line starting with +", identifier, source)
+				return nil // Error
+			}
+		case 8:
+			if is_end_of_line {
+				state = 9
+			}
+		case 9:
+			if is_end_of_line {
+				state = 9
+			} else {
+				// beginning of quality
+				state = 10
+				start = i
+			}
+		case 10:
+			if is_end_of_line {
+				// End of quality
+				q := ch.Bytes[start:i]
+				if len(q) != slice[len(slice)-1].Len() {
+					log.Errorf("%s[%s] : sequence data and quality lenght not equal (%d/%d)",
+						identifier, source, len(q), slice[len(slice)-1].Len())
+					return nil // Error quality lenght not equal to sequence length
+				}
+				for i := 0; i < len(q); i++ {
+					q[i] = q[i] - quality_shift
+				}
+				slice[len(slice)-1].SetQualities(q)
+				state = 11
+			}
+		case 11:
+			if is_end_of_line {
+				state = 11
+			} else if C == '@' {
+				state = 1
+			} else {
+				log.Errorf("%s[%s] : sequence record not followed by a line starting with @", identifier, source)
+				return nil
+			}
+
+		}
+	}
+
+	batch := obiiter.MakeBioSequenceBatch(ch.index, slice)
+	return &batch
+}
+
+func ReadFastq(reader io.Reader, options ...WithOption) (obiiter.IBioSequence, error) {
+	opt := MakeOptions(options)
+	out := obiiter.MakeIBioSequence()
+
+	source := opt.Source()
+
+	nworker := obioptions.CLIReadParallelWorkers()
+	out.Add(nworker)
+
+	chkchan, err := FastqChunkReader(reader, 1024*500)
+
+	if err != nil {
+		return obiiter.NilIBioSequence, err
+	}
+
+	go func() {
+		out.WaitAndClose()
+	}()
+
+	parser := func() {
+		defer out.Done()
+		for chk := range chkchan {
+			seqs := ParseFastqChunk(source, chk, byte(opt.QualityShift()))
+			if seqs != nil {
+				out.Push(*seqs)
+			} else {
+				log.Fatalf("error parsing %s", source)
+			}
+		}
+	}
+
+	for i := 0; i < nworker; i++ {
+		go parser()
+	}
+
+	newIter := out.SortBatches().Rebatch(opt.BatchSize())
+
+	log.Debugln("Full file batch mode : ", opt.FullFileBatch())
+	if opt.FullFileBatch() {
+		newIter = newIter.CompleteFileIterator()
+	}
+
+	annotParser := opt.ParseFastSeqHeader()
+
+	if annotParser != nil {
+		return IParseFastSeqHeaderBatch(newIter, options...), nil
+	}
+
+	return newIter, nil
+}
+
+func ReadFastqFromFile(filename string, options ...WithOption) (obiiter.IBioSequence, error) {
+	options = append(options, OptionsSource(obiutils.RemoveAllExt((path.Base(filename)))))
+
+	file, err := Ropen(filename)
+
+	if err != nil {
+		return obiiter.NilIBioSequence, err
+	}
+
+	return ReadFastq(file, options...)
+}
+
+func ReadFastqFromStdin(reader io.Reader, options ...WithOption) (obiiter.IBioSequence, error) {
+	options = append(options, OptionsSource(obiutils.RemoveAllExt("stdin")))
+	input, err := Buf(os.Stdin)
+
+	if err != nil {
+		log.Fatalf("open file error: %v", err)
+		return obiiter.NilIBioSequence, err
+	}
+
+	return ReadFastq(input, options...)
+}
diff --git a/pkg/obiformats/universal_read.go b/pkg/obiformats/universal_read.go
index 84ca323..bd7660b 100644
--- a/pkg/obiformats/universal_read.go
+++ b/pkg/obiformats/universal_read.go
@@ -148,14 +148,15 @@ func ReadSequencesFromFile(filename string,
 	if err != nil {
 		return obiiter.NilIBioSequence, err
 	}
-
+	log.Infof("%s mime type: %s", filename, mime.String())
 	reader = bufio.NewReader(reader)
 
 	switch mime.String() {
 	case "text/fastq":
-		file.Close()
-		is, err := ReadFastSeqFromFile(filename, options...)
-		return is, err
+		return ReadFastq(reader, options...)
+		// file.Close()
+		// is, err := ReadFastSeqFromFile(filename, options...)
+		// return is, err
 	case "text/fasta":
 		return ReadFasta(reader, options...)
 	case "text/ecopcr2":