OBIJupyterHub/jupyterhub_volumes/web/obidoc/docs/cookbook/illumina/results/unidentified.fastq

@HELIUM_000100422_612GNAAXX:7:1:9007:3289#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atctctc:atctctc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctctc","obimultiplex_forward_tag":"atctctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctctc","obimultiplex_reverse_tag":"atctctc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1204,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCBCCACdUdeeee`[e]aecYV[_D?FECEECEBEEBECEEDEEEEEABEEEEEEEEEEEEEELAEBLCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8649:5297#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":4061,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
atctccagcgagatcgggcaatcctgagccaaatcctgtttttttccaaacaaagattcagaaaacgaaaaaaaggataggtgcagagactcaatgggatctcgctggagat
+
####EEEEEEEEEEEEEEc_cdXa[]aaI[UI\e[Q`Qdfeecab`adeeeeeb_bedced^dgeeaceeeeeQ]Zd_cEEEEEEEEEEEEEEEEEEEEEEEEEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8702:15835#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":4895,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cccatcgctcaccgaaggcgtcaaggaacactgtgcctaacgaggggatgtggctggcttgctggccgcaccccgagttgcaattctatataatctacacgactctcggcaacggatatctgagcgatggg
+
CCCCCCCCCCCCCCCCCCCCCCCEEEEEEED=bbeXa[Y\U`baeeeeabcae_N_G[PZUeT^b`ecc]baebad_\__ebc_ceebbecdJabZ][`[I]ZY^`c]CCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8613:9165#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":101,"pairing_fast_score":0.918,"pairing_mismatches":{"(A:02)->(C:34)":104,"(A:02)->(G:34)":97},"score":3695,"score_norm":0.98,"seq_a_single":7,"seq_ab_match":99,"seq_b_single":7}
ccagagcgagtccattgagtctctgcacctatcctttatgtttatttttgctttctgaagcccttttgtttttctaaaaataaagatttggctcaggattgcccactcgctctgg
+
CCCCCCCEFEEEEDEEEEEEEFEEEEDEEEbbbcbecaY\`Zdee``bYY]adddeec_bXcb\bdbdcdcbedZOWZYZe_``cEEDEDEEEEEELEEECEELEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8670:8750#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4849,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctcgaggctttcaagaacataactaatactactcaagcaaacaaacattaacattcaaaaataatagggtatctaatcctagtaagcctcggagatcggaagagcggttc
+
846?=?:?<6;7>3;=B@\_a__acccafdedeeeeeedeeeeafdbeeddedcbeeefeddeedeeebbea_bdccdUWW[[^^YaEEEEEEEEEEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8644:15229#0/1 {"ali_dir":"left","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":106,"pairing_fast_score":0.893,"pairing_mismatches":{"(A:02)->(G:34)":79,"(A:27)->(G:02)":5,"(C:33)->(T:02)":39},"score":407,"score_norm":0.972,"seq_a_single":2,"seq_ab_match":103,"seq_b_single":2}
ccacacgctctgggcaatcctgagccaaatcacgttttccgaaaacaaacaaagattcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggagagcgtgtg
+
ABDDECA>CC;998;=A>1A>@=<@DD@>D:ADAADDDKD;3@7_VSSOQTCCCC2>A@8A>5AACC>=DCEEEEEEBLEEEE=EEEBEEEEEEEEDEEEEEEEEEEECC
@HELIUM_000100422_612GNAAXX:7:1:8613:5665#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(G:34)->(T:21)":26},"score":4167,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
accgcgctcttccgatctccttcctggttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccccaggaaggagatcggaagagagttta
+
##################EEFFEaZDa^aa^^]]eeeec^dbbdddeegeeeeeeeefeeefaeefeeeeedceecee][acaEEEEFEEEEEEFEEEEEEDEEFFEE##################
@HELIUM_000100422_612GNAAXX:7:1:8647:14028#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":47,"pairing_fast_overlap":59,"pairing_fast_score":0.839,"pairing_mismatches":{"(A:02)->(G:34)":108,"(C:02)->(T:34)":104},"score":82,"score_norm":0.949,"seq_a_single":49,"seq_ab_match":56,"seq_b_single":49}
gcccaccacctagatctacactctttccctcacggcgctcttccgatctccatcagctgcgatatccgttgccgagagtccttggcacatgccgtgccgcgagtatggagatcggaagagcggctcagcaggaatgcggcgacggatatcgtatgcg
+
#################################################CCCACC5<=@CB;;?D=@>cCEABECEBEAEEELEEECEEAEEDCDEEEEEEEELEDAL#################################################
@HELIUM_000100422_612GNAAXX:7:1:8617:21317#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":107,"pairing_fast_score":0.673,"pairing_mismatches":{"(A:02)->(C:33)":76,"(A:31)->(N:02)":31,"(C:24)->(N:02)":42,"(C:30)->(N:02)":35,"(C:31)->(N:02)":34,"(C:32)->(N:02)":13,"(G:32)->(N:02)":10,"(T:02)->(G:33)":85,"(T:17)->(N:02)":43,"(T:24)->(N:02)":41,"(T:25)->(N:02)":30,"(T:29)->(N:02)":39,"(T:31)->(G:02)":15,"(T:31)->(N:02)":32,"(T:33)->(N:02)":37,"(T:34)->(N:02)":40},"score":896,"score_norm":0.804,"seq_a_single":1,"seq_ab_match":86,"seq_b_single":1}
cccagtctcgtccattgagtctctgcacctatcccttttttctcgctttctgaactctggtttgttcgcgtaaaccaggatttggctcaggattgcccacgagactggg
+
CEEEEEBEEJGJJBIDDBBBEEEEEEEBECIIIIHKKLGLBB;8>EEE:E<<>>X__\`Yddc\dYdcdd?DDDDKDDDA<A<<K@:DD@@@@:DDADDDDDDDDDDDB
@HELIUM_000100422_612GNAAXX:7:1:8634:16270#0/1 {"ali_dir":"left","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":94,"pairing_fast_score":0.769,"pairing_mismatches":{"(A:02)->(C:34)":67,"(C:02)->(G:34)":87,"(C:02)->(T:34)":108,"(G:34)->(A:02)":16,"(G:34)->(T:07)":27,"(T:02)->(C:34)":106},"score":2005,"score_norm":0.926,"seq_a_single":14,"seq_ab_match":87,"seq_b_single":14}
tggatatccgttgccgagagtcgtgtggattaaatagcattgcaacatgagggacggctagcaagccagccgtgtccccgcattaggcatagtgttccttgacgccttcggcacgtagtggg
+
CCCCCCCCBCDDCADLDEDa_]^]_^G]]TWcc_c\[L[_ITMPQWWU[_`]VJVX?EE?BCDDEELEEEEDEEEEEEEEEEEEEELEFEEEEEEEELFEEEEEELELCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8682:8238#0/1 {"ali_dir":"left","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":74,"pairing_fast_score":0.803,"pairing_mismatches":{"(A:02)->(G:34)":91,"(C:02)->(A:34)":95,"(C:02)->(T:34)":101},"score":3261,"score_norm":0.946,"seq_a_single":34,"seq_ab_match":70,"seq_b_single":34}
ccacacgctctgatatccgttgccgagagtcgtttgttattttactaagaggccacaacctgcatgcacaccgcaaacggggcaacacaggacgagtccttctcatattatgttttccttggcacatgccgtgcaaagcgtg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC?CCCCe_dbX^daQMNGQ^aaX[`c\__cR_VTXZSO\aa`^cedYc^cb`acaaXaaaEELDEFLELEEELEEEEEEBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8654:20149#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(C:02)->(T:18)":102,"(T:02)->(G:34)":97},"score":5099,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
atctccagcagcgtagggcaatcctgagccaaatcctctttttagaaaacaaaggttaaaggaaaagaaaagggggataggtgcagagactcaatggtacgttgctggagat
+
C@CCacc_aaa_]RQJRO^aa]V__cc_ceaebeed`ededed`dddeeeddd]eeefece\bdddeb`dedeeeec_dEEEEEEEEEEEEEEEEELEEEE<EEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8647:3650#0/1 {"ali_dir":"left","ali_length":88,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":88,"pairing_fast_score":0.953,"pairing_mismatches":{"(T:06)->(A:34)":93},"score":5419,"score_norm":0.989,"seq_a_single":20,"seq_ab_match":87,"seq_b_single":20}
ccacgatcgcgccgaaggcgtcaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatccgcgatcg
+
CCCCCCCCCCCCCCCCCCBCU_baV_a_eeededdd_dRSQOQ^eeee^NZ^[XaZa`adccdaddbedeeeeaeedb]ebe_eaNRRQQU]GYYWaaa\S^O^]e_eCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8632:16185#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":103,"pairing_fast_score":1,"score":6548,"score_norm":1,"seq_a_single":5,"seq_ab_match":103,"seq_b_single":5}
catctccacagcatgcgcacggcatgtgccaaggcgggaagcgccttcagcgtgatgtcccaggcttcgcgccaggagactctcggcaacggatatctcgagctgtggagatc
+
###A:\cHZWW]X^Tbebb_\ba[bcc`_eNeTdeddeebedebecedacecfceccbc_b\___beb`ec`c_cL]]\Y^]]]]X\^__YZXYZdd^UZa]I[aacJ=A###
@HELIUM_000100422_612GNAAXX:7:1:8650:17434#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(C:34)":102},"score":6264,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagagcgagtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccactcgctctgg
+
CCCCCCb__dcWdcbeJeO^^^]ZRd\Tdeeceeaeeeeedeeeeebeeededebdeeddedeecdeedeeddebddeeeccb_d`ccaIaa[_aN_aEEELEEEEEECCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8646:6034#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4173,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gccacccacgagatcttcactctttcccctcacgacgctcttccgatctccagtgcaacaccaactctttcattcagacgtatacgtatgtatatacggtgatcatggagatcggaagagcggttcagcaggaatgccgagacggatatcgtatgcg
+
###############################################AA`c^`dZdddddc^ceeeed\eeabeeeeeeeefeefedeeeffeedeccedeed_UX[Z;A=:7:=A=A#######################################
@HELIUM_000100422_612GNAAXX:7:1:8614:14696#0/1 {"ali_dir":"left","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":83,"pairing_fast_score":0.875,"pairing_mismatches":{"(A:02)->(C:34)":103,"(C:02)->(A:33)":105,"(T:02)->(A:34)":91},"score":2575,"score_norm":0.964,"seq_a_single":25,"seq_ab_match":80,"seq_b_single":25}
ccaacgagcattcggttggggcgaccagtgaataaataaatcaccacttattttaagatatctttatctcatttaagaccctgttttataaagatcaaaaaccaagctaccttagggataacagcggcgttgg
+
CCCCCCCCCCCCCCCCCCCCCCCCDEEEEEaa_[cbe`a`M]_X^ZWN^dcdadSbedeeY_\_acc]b_FEEDDDCAECEEEEEEEEEELECEEEEEEEEELEKEEECCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8619:5417#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":107,"pairing_fast_score":0.712,"pairing_mismatches":{"(A:34)->(G:02)":6,"(C:34)->(G:02)":10,"(G:02)->(C:34)":84,"(G:34)->(A:02)":7,"(G:34)->(C:02)":15,"(G:34)->(T:02)":23,"(T:02)->(A:34)":103,"(T:18)->(A:34)":54},"score":2535,"score_norm":0.897,"seq_a_single":1,"seq_ab_match":96,"seq_b_single":1}
tccagagtacgcgggcaatcctgagccaaatcctttttttttggcaaacaaataaagattccgaaagaaataagggataggtgcagagactcaatggcgtcgatctgga
+
#EELLLLEELLEEELEEEEEEELEEEEEaX[`V[[[a_edddBb^`acX\`TRD^]RLQRR`X[^^cYc[]\\\aEEEEEEEELEEEEEEEEEEDEEEEEEELEDEDE#
@HELIUM_000100422_612GNAAXX:7:1:9054:7902#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gatctac:gatctac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatctac","obimultiplex_forward_tag":"gatctac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatctac","obimultiplex_reverse_tag":"gatctac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4398,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ccgtcta
+
eee_edb
@HELIUM_000100422_612GNAAXX:7:1:8616:10136#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(A:02)->(C:34)":101,"(A:02)->(G:34)":94},"score":5462,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
ccagcacagctccattgagtctctgcacctatccctttttgattttaactttctgaacttttatttgttttcagaaagaaggatttggctcaggattgcccagctgtgctgg
+
CCCCS[XU_ccTY]\U[b_cdb^UUa^]_a_cebec`eb_Ta`eeee]deeeeec`dbbdeed`cddbec][]cc`]dcc[\[[^aaEEEEEELEEEEEELEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8700:8418#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":103,"pairing_fast_score":0.92,"pairing_mismatches":{"(A:02)->(G:34)":87,"(T:34)->(G:26)":9},"score":4698,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
catctccatcgtcgcagggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggagatc
+
####A\`WCL]`VcXVe`_b\beXdc\cdV]XU^^WU`c]eeeaeaddeeedc[\b^`_c`ee_\_\[T_cdacca_ZVcEEEFEELEEEEEEEEEEEEEEEEEEEEE#####
@HELIUM_000100422_612GNAAXX:7:1:8650:10242#0/1 {"ali_dir":"right","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":79,"pairing_fast_score":1,"score":5492,"score_norm":1,"seq_a_single":29,"seq_ab_match":79,"seq_b_single":29}
tctttccccacacgacgctcttccgatctccacagctcgagggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaaaagatcggaagagcggttcagcagaaatgc
+
#######################A:???=_bYbY`dfaT^YU]c_dcac_bf[eeced^edeefeeeee[beeedddedbeddeeeb]feeed_\^^b_b`cddddddB############################
@HELIUM_000100422_612GNAAXX:7:1:8626:18323#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":5073,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
accactctcctccgatctccttatgtgggtcgccccaaccgaatctattatatgtaagggtattttacatggttgataaagctctatagggtcttcttgcacataaggagatcggaagagcggtgc
+
###############A??UYKTZ_edeaeeeec_`cc_ccRc_cdceddeeebaeeeccceeeeabceb`bZb\aZb`dRb^d_bdd_aZaYcEEDDEEEEEEEDEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8671:17914#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":107,"pairing_fast_score":0.894,"pairing_mismatches":{"(G:02)->(A:28)":95,"(G:34)->(T:17)":34,"(T:02)->(G:34)":98},"score":5048,"score_norm":0.972,"seq_a_single":1,"seq_ab_match":104,"seq_b_single":1}
cccagtctgcagggcaatcctgagccaaatcctggtttacgcgaacaaaccagagttcagaaagcgagaaaaaagggataggtgcagagactcaatggtgcagactggg
+
CEEEEEab[bcce_ccebaccVc_eeedec^ZbD`]`X`e_f_cdeeeeeddebebec_beeebbdde]bee_Zdcdd`aEEEEEEEEEEEEEEFEELEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8673:15735#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(G:34)->(T:02)":14},"score":4322,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccacgtcagtcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggactgacgtgg
+
CCCCCCEEEEEEELEEEEEEEEaUbaaee]]eeeaaaU]ecddeeeedcdeceedbeefceeddd]eee`c_aVa_ddc_c]UcEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8678:10187#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":92,"pairing_fast_score":0.899,"pairing_mismatches":{"(A:02)->(C:32)":102,"(A:02)->(C:33)":98,"(A:02)->(G:34)":108},"score":2793,"score_norm":0.967,"seq_a_single":16,"seq_ab_match":89,"seq_b_single":16}
ccgcgcattattcggttggggcgaccgtggattaatcatcctcacctatatagatatatttatcatttactgaccctttaaaagatcatcaataaagctaccttagggataacagaatgcgcgg
+
CCCCCCCCCCCCCDCCEEFEEEEEEEEEEEEEEEEEEEEEEEDEfdaeefegeecef`edPda`d]c\c^TWZY[b`caacdabbDEEEEECEEEEEKEEDJEECEELCCACBCCCCCCACCCC
@HELIUM_000100422_612GNAAXX:7:1:8984:14076#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:34)":65,"(C:02)->(A:32)":59,"(C:02)->(G:34)":56},"score":1765,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
BBCCCCC=CCCCCCC@??=_Y_`eeebbceeeb^Z_dbS`__^^]aEEBBCDEECLECJE@EEELEEEEDCEEEEELEEELCCCCCCCCCCCCCDCCCCB
@HELIUM_000100422_612GNAAXX:7:1:8621:4087#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5935,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
tctccacagcgacaccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaacgtgatttggctcaggattgccctgtcgctgtgaga
+
###EEEEEEEFEEEEEEEeZece^ddd_ebaeeee_aeeeeebbadcdbddeeeebbdebabOdeedddd^deeeeUaa\]baeZ`ec\\^\\YVXYTQRQNRUY]`^C8A
@HELIUM_000100422_612GNAAXX:7:1:8627:16041#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4734,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccatgctgctggggcaatcctgagccaaatcacgttttccgaaaacaaacaacggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggcagcagcatgg
+
CCCEEEEEEEEEEEEEEEFEEac_cc`]^]acdL`cdedf`cdeeeedddced_eeeaceecede_acc_[Y[\]ecebb\[YU\[YY[]cEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8627:18657#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5663,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacacgacgctcttccgatctcctaagtctggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaagaagatcggaggagcgttcagcagaa
+
#####AAA608(4@A@@?;9<<5Cdaedb^ddbZaa_aa[ccXde`edceeedddebeeddeeeedecedcceedeeed_bdd^cc]c_XZYYWbcc`\ZXYZXaYaE########################
@HELIUM_000100422_612GNAAXX:7:1:8616:1574#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":103,"pairing_fast_score":0.94,"pairing_mismatches":{"(A:34)->(G:02)":11,"(C:02)->(A:34)":108,"(C:34)->(A:02)":12},"score":1778,"score_norm":0.971,"seq_a_single":5,"seq_ab_match":100,"seq_b_single":5}
ccagatcgcgaccattgagtctctgcacctatccttttcctttggattctagttcgagaaccacttgttttctcaaaacacggatttggctcaggattgccctcgcgatctgg
+
CCCCCEEEEELLEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE=?E_bc\Zece\abb`ceb[_bd`eeS[[F?EEFEBCEEFCEEEEEEEEEE:EEEEEEEEEEELCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8693:1415#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":85,"pairing_fast_score":0.72,"pairing_mismatches":{"(A:02)->(C:34)":97,"(A:33)->(G:02)":53,"(C:02)->(G:34)":96,"(C:34)->(G:02)":24,"(G:02)->(T:34)":105,"(G:34)->(A:02)":36,"(G:34)->(C:02)":25,"(T:19)->(G:02)":68},"score":398,"score_norm":0.906,"seq_a_single":23,"seq_ab_match":77,"seq_b_single":23}
ccacgatgcaggatatccgttgccgagagtcgtgtggattaaatagcattgcaacacgaggggcagctagcaagccagccgcttccccgggttaggcacagtgttccttgacgccttcggctgcatcgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCLLEEEECEDEEDLE@EBEEDEDEEBEEDAKBEEEEDEB@ECA=C=?@CCZUaWcaaEEEBCEEEEEEEEEEELLEEEEEEELEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:2482#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":108,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(C:34)":84,"(A:02)->(G:34)":108,"(A:34)->(C:02)":16,"(C:02)->(G:34)":97,"(C:34)->(T:02)":31,"(G:02)->(A:34)":85,"(G:02)->(C:34)":101,"(G:02)->(T:34)":82,"(T:02)->(C:34)":90},"score":1397,"score_norm":0.907,"seq_a_single":0,"seq_ab_match":98,"seq_b_single":0}
ccagagtacgcgggcaatcctgagccaaatcctttttttttttggcaaacaaataaagattccgaaagaaaaagggataggtgcagagactcaatggcgtcgatctgg
+
EEEEEEEEEEEEEEELEEEEEEEEEEEEEELEEbbddedddd\da\Kc_YdeZ\eEEEEEEEEDDDDEEEEEEEEEEEEEELELLEEEELEEEEEELLEELEEEEEEL
@HELIUM_000100422_612GNAAXX:7:1:9388:6567#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (taccgtg:taccgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taccgtg","obimultiplex_forward_tag":"taccgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taccgtg","obimultiplex_reverse_tag":"taccgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(T:02)->(C:28)":54},"score":1873,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCC@CCCCCCCCCCCCC@CcSd`eceb_ebe``aPS__ZYc`Ubdbb;5@>;;FEEDBEAE?EEEEEE@EEEEEEDEEECEBCCCCCCCCCCC@BCCCC?
@HELIUM_000100422_612GNAAXX:7:1:9393:8196#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agatcta:agatcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agatcta","obimultiplex_forward_tag":"agatcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agatcta","obimultiplex_reverse_tag":"agatcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":3120,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaactattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCA`WcTBccc[Yee\`c^bZcVOZ_S[[^_X@X\ZT_QY]\[M^MZ\RMRRQMVZXZbc_QaEEECBCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8734:7406#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atgatca:atgatca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgatca","obimultiplex_forward_tag":"atgatca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atgatca","obimultiplex_reverse_tag":"atgatca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:02)->(G:34)":56},"score":546,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCAEECEBc^[Sc]UccEE=EBAEE@AC???C8C;?ECELC?LDCDEEEEEEEEEEEEEELBEELCCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8621:11178#0/1 {"ali_dir":"right","ali_length":48,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":45,"pairing_fast_overlap":48,"pairing_fast_score":1,"score":3529,"score_norm":1,"seq_a_single":60,"seq_ab_match":48,"seq_b_single":60}
ttttaaagatacggccaccaccgagatctacactctttccctacacacgctcttcgatctccacgcagctcgatatccgttgccgagagtcccttggcacatgccgtgagatcggaagagcggttcagcaggaatgccgagacggatctcgtatgcggtcttctgctt
+
##############################?<B8;0@;77;+8;88(96A4?6@CAACBCeedeeeeeebeeeeeceeffebeeeeeeeeeeeebceeee`ceeeeeeCCCC;BCC<CCC@<BA>57:87?<?6998+9<AAA0A#######################
@HELIUM_000100422_612GNAAXX:7:1:8617:4785#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":105,"pairing_fast_overlap":108,"pairing_fast_score":1,"score":6983,"score_norm":1,"seq_a_single":0,"seq_ab_match":108,"seq_b_single":0}
ccagagtagcggggcaatcctgagccaaatccttttttcgcaaacaaacaaagattccgaaagctaaaaaaaaaggataggtgcagagactcaatggcgctactctgg
+
Zc`_dccd]bZbeeea`eeece`beeeeecXe]efeeeeed`eeddeddeeddeeedeefedebddddeeedd`dececc]^`]UY]ZYRaUU^\ZZV]Y_cEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:8860:10997#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agccata:agccata)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agccata","obimultiplex_forward_tag":"agccata","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agccata","obimultiplex_reverse_tag":"agccata","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(a:02)->(t:34)":81,"(a:07)->(t:32)":48,"(c:02)->(g:34)":77},"score":1412,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctc
+
@@B?BBBBCCCCCCCCC?CDLBCDEDEE6@@@@@@:E?1QS=\ba^\EX`]]W^PNXeab]ZaMa=DEDEEEEAEDL?BBLACC;CC?CCCCA=C@CA@?
@HELIUM_000100422_612GNAAXX:7:1:8641:18932#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(C:02)->(A:34)":94,"(G:23)->(A:34)":59},"score":4496,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
cctacacgacgctcttccgatctccactcgctacgggcaatcctgagccaaatccttaagttaaaataaagaaaagataggtgcagagactcaatgggtagcgagtggagatcggaagagcggttcagcag
+
CCA//00-==;>==8A>?+>=?=cdccceebeaeb`cebbdcefeeedeefeedeeeeCcfdddeeddcddeedddde\\\Z]SYXZ[aEFEELEEEEEEEEEEFEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:8621:5606#0/1 {"ali_dir":"right","ali_length":76,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":73,"pairing_fast_overlap":76,"pairing_fast_score":1,"score":5431,"score_norm":1,"seq_a_single":32,"seq_ab_match":76,"seq_b_single":32}
cactctttccctacacgacgctcttccgatctccacagtcgagccattgagtctctgcacctatcctttttgattttaacttaaggatttggctcaggattgcccctcagatcggaagagcggttcagcaggaatgccga
+
##########@-2BBBBBC<>==<ABCB@8A;cccdab__ce`dcbecdedeeeedaebeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeed\a_aaeececaccccE################################
@HELIUM_000100422_612GNAAXX:7:1:8634:20734#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":69,"pairing_fast_overlap":105,"pairing_fast_score":0.676,"pairing_mismatches":{"(A:33)->(G:02)":12,"(A:34)->(G:02)":8,"(C:02)->(G:30)":85,"(C:02)->(T:34)":93,"(C:30)->(T:02)":36,"(T:16)->(G:15)":63,"(T:30)->(G:02)":18,"(T:34)->(C:02)":27},"score":2368,"score_norm":0.914,"seq_a_single":3,"seq_ab_match":96,"seq_b_single":3}
tctccacacgcagaccattgagtctctgcacctatccttttttcaccttctaggcctttgtttgtttttggaaaataggatttggctcaggatgccctctgcgtgtggaga
+
###EELELEEBKEEEEAHAEEEEEEELDEEE@EDAH]^bddc`XOWP^RZR_TUZXOZLNOG2VZ_PRNRIT^[M^a]__WMbBHAEEEAEEL@DA@DE9DEEEEEEE###
@HELIUM_000100422_612GNAAXX:7:1:8680:20579#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":105,"pairing_fast_score":0.755,"pairing_mismatches":{"(A:32)->(G:02)":47,"(C:29)->(A:02)":4,"(G:16)->(C:02)":13,"(G:25)->(A:02)":54,"(G:30)->(T:02)":41,"(T:32)->(C:02)":37},"score":587,"score_norm":0.933,"seq_a_single":3,"seq_ab_match":98,"seq_b_single":3}
ccacgagcacagggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggtatgctcgtgg
+
>>>G;;;@<@AA:AAA=A;ADDBBDDDJDDCADD>AJC?AH+CAADJ???@C1CC=AA7>DDDDCC<CC=C^YR]PAN<GBP>;LZ2<>BA:80::,>?:>3>5666>1<<
@HELIUM_000100422_612GNAAXX:7:1:8676:2768#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4188,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctacactctttccctacacgacgctcttccgatctccgtgagccccgtatatacatacgtatacgtctgaatgaaagagttggtgcaagccaggagatcggaagagcggttcagcaggaatgcggagaccgatatcgtatgcg
+
#########?=?=<=;1A<CCC@AAA?A-5?3<=BCACDBCCCCCACCCeeeeebeeeeeeedeeeeeeeeeeeeeeeeeededeeeeedeeee^^^`a\[]Z[[___?AA=8=:BABB######################################
@HELIUM_000100422_612GNAAXX:7:1:8612:8399#0/1 {"ali_dir":"left","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":106,"pairing_fast_score":0.845,"pairing_mismatches":{"(C:02)->(G:29)":80,"(C:02)->(T:33)":101,"(C:21)->(A:02)":8,"(T:02)->(G:29)":86},"score":2077,"score_norm":0.962,"seq_a_single":2,"seq_ab_match":102,"seq_b_single":2}
tgcaccccttgagtctctgcacctatccatttttctcgtcgctttcttaactgaactttcccttgttttcgtaaagcaggatttggctcaggattgcccgtgcatgctgg
+
BBDDDDD?D@22112DDDDDDDDDDDD`RL`KQ\`RO_G[V`^RX^]^WZVH?HGYELL7[TKL_bbbBDCDCDCC8DCG@8@DDGD;@C@D@@D@:@ADK??DDDDDBB
@HELIUM_000100422_612GNAAXX:7:1:8673:9717#0/1 {"ali_dir":"left","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":74,"pairing_fast_score":0.887,"pairing_mismatches":{"(A:33)->(G:02)":42,"(A:34)->(G:02)":48},"score":1690,"score_norm":0.973,"seq_a_single":34,"seq_ab_match":72,"seq_b_single":34}
ccacgtgcgcgcacggcatgtgccaaggaaaacaataaacgagaaggactcgacttgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatcgcgacacgtgg
+
CCCCCCCCCCCBCCCCBCCCCCCBCCACCCCCC@BBEEEEEKEEEEDLEDC^[aabe\W^ZUYV`ac`d`R_[Ycec:FEEEDEEEEEDDABEDEEE?EEEEE?EDEBCCCCACCCCCCCCCCCCCCCCCCCDCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8699:14791#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5467,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cccacacgacgctcttccgatctccgcgatgaggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgtcatcgcggagatcggaagagcggttcagcag
+
6803<;<0:@@ACA@?==2<<A=cccccee_edddedeeZedeeccecdededeeeeebdcefebdeeeebeeeecedeccUecbdcd_cc_c_bca`cEEFEEEEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:9181:13392#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(c:02)->(a:31)":68,"(c:30)->(a:02)":41,"(t:02)->(g:34)":76},"score":712,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCLEEEEEEEEDEEEDD=C=C?CCH:ECZ]YYSUSRX[VVW>A@8?EEAEEIEECCBECLCEAEECCCCC8?CCCCCA?C?CC
@HELIUM_000100422_612GNAAXX:7:1:8659:19826#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":85,"pairing_fast_score":0.951,"pairing_mismatches":{"(T:34)->(G:07)":32},"score":5235,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
ccgtcacgacgctcttccgatctccgcgcattcaagaagaccctatagagcttttttaataaatcaataatactgatcaaaaattcggttggggcgaccaatgcgcggagatcggaagagcggtgcagcag
+
#########??3:;<';?.()B@_ceeeRRQGNd^^_de_Zb`eeecbbT_eebaedaX\`aYeceeefeec]]_Vc]__]^[cL_]_ZZYWWYZWMXT]Y[[]`]]LA?#####################
@HELIUM_000100422_612GNAAXX:7:1:8622:13344#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:34)->(G:02)":29},"score":3498,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tccgatcgcccactgtcgagggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatggtcgacagtgggagaccgga
+
########EEEEEEEEEE>EEEEEEEDELDEEEEEEEEacbcNddda`d`ee`beeceb_eed_`aUae]ebea_`bdXYPPZY\[^[b]aEEEEEEFEEEEEEEEEE########
@HELIUM_000100422_612GNAAXX:7:1:8914:9631#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtattc:tgtattc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtattc","obimultiplex_forward_tag":"tgtattc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtattc","obimultiplex_reverse_tag":"tgtattc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:34)->(T:02)":19},"score":1112,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCC@CCCCCCCCCCBCCCL^^da\_VOWcUdd]]`aXCCC<A>><5A=>99=EE?CAEEECEEEEEEECEDELEELEBELECCCCCCCCCCBCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8682:6824#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5719,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacgcacgtaccattgagtctctgcacctatctttttttcttttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccctacgtgcgtgg
+
CCC_\_e^cee\_`a_adcfde`Zcecc_ccddbdbdccdd\dddddbfeedce_cc`cddbab[ddeddYZ`acccbU]ZXY\ZcEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8646:10752#0/1 {"ali_dir":"right","ali_length":67,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":67,"pairing_fast_score":1,"score":4911,"score_norm":1,"seq_a_single":41,"seq_ab_match":67,"seq_b_single":41}
cgagatctacactctttccctacacgacgctcttccgatctcccactagcgtgatatccgttgccgagagtcccactgtgttccttgacgccttcggacgctagtgggagatcggaggagcggttcagcaggaatgcggagaccgatat
+
77A6:>9<;9:96A535/A@9ABAB8AB=AAA8:78-)@CCedefebceeedeeeedeedeeeeeeeeeeeeeeeffeeefefeeeeddebeefbeee]eabbdadeeCCC@;<@BCCCCC@@CC########################
@HELIUM_000100422_612GNAAXX:7:1:8703:4967#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5800,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccatcacgtcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccgacgtgatgg
+
CCCCCCTcdeXXaf_ece^baba_ccbcc]aaN]beaeeeeedbeeeeefe`eebeeeceeeebddaeeeedde]]baabc`aZ_ccccEEEEEEEEEEDEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9346:3962#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{},"score":1199,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCDCCCCCCCBBCEEEEEEEEEa`bad]WX`_dc__a__aEE:<EDEEBCAE:EBEEDEEEBCEEAELEELEAEHC>BCCC>CCCCCC.CC?B<
@HELIUM_000100422_612GNAAXX:7:1:8842:15635#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (taatcat:taatcat)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taatcat","obimultiplex_forward_tag":"taatcat","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taatcat","obimultiplex_reverse_tag":"taatcat","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(A:31)":59,"(C:02)->(G:31)":56},"score":1131,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCDCCCCCBCCCCC?><]Z][LbaX]eXeeaWVR^\C=C4EEEEEA<A8=CD7ICEIEAAEEEEEECEEEEFE?LEEEECCCCCCBCCCCCD@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8662:9899#0/1 {"ali_dir":"right","ali_length":80,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":80,"pairing_fast_score":1,"score":5573,"score_norm":1,"seq_a_single":28,"seq_ab_match":80,"seq_b_single":28}
ctttccctacacgacgctcttccgatctccagacgagagccattgagtctctgcacctatccgctccggcaggaacggacttggctcaggattgcccctctcgtctggagatcggaagagcggctcagcagcaatg
+
##############@0::@CACCCACD@eeeRdedd^dYbbZ]beedeeadeee`eeeecefOeeebebee_cbeee_bdededddece`c_dcbceb[Vb[a]dUed?C=85==>)?>=?###############
@HELIUM_000100422_612GNAAXX:7:1:8631:7464#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":69,"pairing_fast_overlap":81,"pairing_fast_score":0.885,"pairing_mismatches":{"(A:34)->(G:02)":43,"(C:02)->(T:30)":91,"(T:02)->(C:34)":108},"score":1078,"score_norm":0.963,"seq_a_single":27,"seq_ab_match":78,"seq_b_single":27}
tacgatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcgtttattgttttccttggcacatgccgtgcgtacgacgtgg
+
CCCCCCCCCCCCCCCCCACCC@CCCCCEDECEEBEDBEBBECLEEDEFEBCEa]]]_Xc\Z`\\a`W`[DECEBECEEDDEEEE?EBCEEHEBEEEEEDDEEDEEEELCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9084:8915#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtaatta:gtaatta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtaatta","obimultiplex_forward_tag":"gtaatta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtaatta","obimultiplex_reverse_tag":"gtaatta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1277,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagttctaaacccaaatagttcaaccaacaaaactattcaccagagtactacaagcaacagcttaagactcaaaagacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCCCCCDa`ea[^SXYeafee`^Y``DEDDEEEEEEDCEEEEEEEDEEEEEELEEEEEEEEEEEEEEELCCCCCCCCBCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8651:9749#0/1 {"ali_dir":"left","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":91,"pairing_fast_score":0.898,"pairing_mismatches":{"(A:02)->(G:34)":108,"(C:02)->(A:34)":94,"(T:02)->(G:34)":102},"score":3646,"score_norm":0.967,"seq_a_single":17,"seq_ab_match":88,"seq_b_single":17}
cccgacgtgatccattgagtctctgcacctatcctttttttttcttgcttttttattatttttttcttttgagtattttttgcttttgacaaaaggagttggctcaggattgcccacacgtcggg
+
CCCCCCA@CBCCCCCCCEEEEEEEEEEEEEeba`Vdddddb`bbb`Jcd`dcdedZPS``d___Ybbbb`X\\Madeee`RSNaaaaEEEEEELEEEEEEELEEEEELCCC@CCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8980:4326#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcggtc:ggaggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcggtc","obimultiplex_forward_tag":"ttcggtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggaggaa","obimultiplex_reverse_tag":"ggaggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"score":1975,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataatacgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCDBCCXaceZ]e``ebdeebb^daXY__\_Y\^R]AEAECBBEEEEEDEEEEEEEEEEEEEELCEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8615:8733#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(G:31)->(T:06)":41},"score":3520,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccacgtcgaatgggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttaagaaagcgaaaagaaaaaaaagataggtgcagagactcaatggcgtacacgtgg
+
BBBBDDDDDDD@BDDDDDBDDDDDDDDDDDDDD`W`\QQPDL]dc_UY\_a`Z_U]UbQaadd[[`__``aa\\\[__Z\XS^aZR[XSZa`aEEDEE@EDEEDEDEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8642:20367#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":87,"pairing_fast_score":0.905,"pairing_mismatches":{"(G:32)->(A:06)":56,"(T:34)->(G:23)":44},"score":4344,"score_norm":0.977,"seq_a_single":21,"seq_ab_match":85,"seq_b_single":21}
ccaagcgagtgatatccgttgccgagagtcatatttttttctgttggcctcggcagccgacggagccctcgaagggctcgccggtgccttagctttaccgtgttccttggcgtcatcccagagctctgg
+
CCCCCCCCCCCDCBCDCBCCCEEEbccWU___[Y\\eaX^`SUCYJWHULKQSYLEVUS`OZcaac_``eedeYee^fbe_`aedc^cc^dea]ZcbbEEEEEEEEEECCCCCCCCCCCCCCCCCCCBC
@HELIUM_000100422_612GNAAXX:7:1:8629:21150#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":102,"pairing_fast_score":0.808,"pairing_mismatches":{"(C:02)->(T:34)":99,"(G:34)->(T:09)":25,"(G:34)->(T:11)":19,"(G:34)->(T:16)":17,"(T:34)->(G:14)":20,"(T:34)->(N:04)":36},"score":4880,"score_norm":0.941,"seq_a_single":6,"seq_ab_match":96,"seq_b_single":6}
ccagtcactgcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccgcagtgactgg
+
CCCCCCbede]eafXZDSEEQMRRFQRRQ\[[]\QIRSJ_]\_Xdeeaedeecce^ddbde^e]bbdeaceddeeaaeced`bWXa`caQ]cEEEEEELEEDEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8687:16530#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":106,"pairing_fast_score":0.961,"pairing_mismatches":{"(A:02)->(C:34)":103},"score":4443,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccactgcacgtccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgcccacgtgcagtgagg
+
##EEDEEEEEEEEEECDEEe^cccc`Rcb[`dbZZadf`bcebX]_aaebfeab`bedbd[cdfdddddaec[Y\Y[VZXZZa[NaEEECEEEEEEEEEEEELEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8686:4525#0/1 {"ali_dir":"left","ali_length":88,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":73,"pairing_fast_overlap":88,"pairing_fast_score":0.859,"pairing_mismatches":{"(T:02)->(A:34)":87,"(T:02)->(C:34)":100,"(T:02)->(G:34)":104},"score":4264,"score_norm":0.966,"seq_a_single":20,"seq_ab_match":85,"seq_b_single":20}
ccaacggaactgttatccctaaggtagcttttgtttgtagcctgttgtacagggtctgtagttggatatatttatctttttttataaggatgagttatccttggtcgccccaaccgaatttccgttgg
+
CCCCCCCCCCCCCCCCCCCDEEEbaba\ddeeceddcbbd^bccdacddd]^cb\eaeb_^__`Zceeeeeebdabbccddd\dEELEEEEEEEEEEEELEEELEEEECCCCCCDCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8665:5689#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":102,"pairing_fast_score":0.828,"pairing_mismatches":{"(A:02)->(T:34)":108,"(A:34)->(G:02)":16,"(C:02)->(A:34)":100,"(C:02)->(G:34)":103,"(C:34)->(A:02)":25},"score":3147,"score_norm":0.941,"seq_a_single":6,"seq_ab_match":96,"seq_b_single":6}
ccagcgactgtgggcaatcctgagccaaatccatgttttgagaaaacaagggggttctcgaactagaatacaacggaaaaggataggtgcagagactcaatggacagtcgctgg
+
CCCCCCLEEDEE;EELEBEEEBE?LeaaeXaeWeeeZb[^cV\_`^_QKFYW]YYb_eeIMPQPKFYWUbVcV]_Ma`]TaEFEEEEEEDEEEEEEFEELEELEEBDLCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8611:20183#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(T:02)->(G:34)":103,"(T:34)->(G:10)":18},"score":5263,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagtcacgaggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggagcgtcatgg
+
CCCCCCEEEeeeeba`aF]edWcedeeee^edeeXccc_eebbeeeecd^eceeceedbdfedddbdeed____ceeeeeeeecEEEEEEEEEEEEEEEEEELEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8696:7996#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":104,"pairing_fast_overlap":107,"pairing_fast_score":1,"score":4915,"score_norm":1,"seq_a_single":1,"seq_ab_match":107,"seq_b_single":1}
cccagcatgaggggcaatcctgagccaaatcctggtttacgcgaacaaaccagagttcaaaaagcgagaaaaaagggataggtgcagagactcaatgggactagctggg
+
CEEEEEEDEebeebc_`_XeZeec[ebeebceecbc_Z_deeeddeeeedef]eecceeTeeeYcb`c^cY^WT[eeb^cEEEEEEEEEEEEEEEEEEEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8919:21074#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(c:31)->(a:34)":73,"(c:32)->(a:02)":41,"(t:24)->(a:02)":34},"score":1160,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCBCCCCCCCCCBCEEKEEE>EEEBEBB@B?C?C6CJCCEAE=BADD?CaY]RddXaaa_c]c_cZeeCE?EEEEEE@CCCCC?CCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8652:5640#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":85,"pairing_fast_score":0.854,"pairing_mismatches":{"(A:02)->(G:34)":96,"(G:09)->(C:34)":80,"(G:35)->(T:07)":37},"score":3657,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
cccatctacgcgatatccgttgccgagagtcgtgtcgattgaatagcattgcaaaacggggggcagcaagcaagctagccgcgtccccggattaggcacagtgttccttgacgccttcgggcgtagatggg
+
CCCCCCCCCCCCCCCCCCCCCCCEEEb^WdcZ^^^WHUZ]Q_c___cbdeaZa_c_]Xcddbe^dWPYYZRRRPOWYZXFcc_]adcXdEEEEEELEEEEEEEEEEEECCCCCCCCCCCCCCCCBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9356:13499#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcggtc:ttcggtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcggtc","obimultiplex_forward_tag":"ttcggtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcggtc","obimultiplex_reverse_tag":"ttcggtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":63,"pairing_fast_score":0.683,"pairing_mismatches":{"(A:30)->(G:02)":28,"(A:31)->(G:02)":30,"(A:34)->(T:02)":23,"(C:02)->(A:34)":58,"(C:34)->(A:02)":22},"score":480,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
CCC3?<??CCCCCACCBCEEELLEEDEHBICDCdZaDXX_T]6>=?BC@=CA>>AC8LEEE8DEEE?EEBCEEEELBEEEECCCCCCCCCCCCCCCCC>
@HELIUM_000100422_612GNAAXX:7:86:4295:5054#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":59,"pairing_fast_score":0.929,"pairing_mismatches":{"(A:23)->(C:06)":65},"score":3436,"score_norm":0.983,"seq_a_single":49,"seq_ab_match":58,"seq_b_single":49}
gcgaccaccgatatccactctctttccctacccgaccctcttccgatctccgcctcctttagataccccactatgcttttctagaggagcctgtttctaaggaggcggagatcggaagagcggttaagcaggattgccgcgacagatctcttatgcg
+
#######################################?>;>:;:9<)ddR`daeeec_]YaU<Y]aebcabeUbabbbcbceS[NN[cbbb[^WS[`daYbbEEEE#################################################
@HELIUM_000100422_612GNAAXX:7:1:8653:10074#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":105,"pairing_fast_score":0.873,"pairing_mismatches":{"(A:32)->(G:02)":14,"(C:02)->(A:33)":94,"(G:33)->(T:08)":25,"(T:07)->(C:02)":4},"score":3140,"score_norm":0.962,"seq_a_single":3,"seq_ab_match":101,"seq_b_single":3}
ccatgtactgcccattgagtctctgcacctatccttttttattttcgctttctgaaccttcgtttgttttcggaaaacgtgatttggctcaggattgcccgcagtacgtgg
+
>>>1=@<=@;DDDJDDDDacc\`\E[V\_Y_[LTTLJYPc^UXZ\]Y\c`bcccV`bZa^Y[PW\c[b^ZYcDDDD<DDDDDDDDDDDDDDDDKDDDDDD<<DCCD@@BBB
@HELIUM_000100422_612GNAAXX:7:1:8888:19765#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ggaggaa:gcgatga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggaggaa","obimultiplex_forward_tag":"ggaggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgatga","obimultiplex_reverse_tag":"gcgatga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4439,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cgcgaca
+
eeedeea
@HELIUM_000100422_612GNAAXX:7:1:8649:15770#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(A:33)->(G:02)":27,"(A:34)->(C:08)":39,"(G:34)->(A:02)":25,"(G:34)->(T:02)":26},"score":3464,"score_norm":0.953,"seq_a_single":23,"seq_ab_match":81,"seq_b_single":23}
ccacacgagcaccgaaggcgtcaaggaacactgtgtctaacccaagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatcgaatgtgctgg
+
CCDCCCCCCCCCCCCCCCBCCCCELLKcdad_Xc_`]\F[XeaMeeb\\__`babWR\XBW`aKS^_b`eeddMacdcP`ccZaEC?EEEECEEEEEEEEEEEEEEEECCCCCCDCCCCCCCCCCCCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:2105#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":87,"pairing_fast_score":0.833,"pairing_mismatches":{"(A:02)->(C:34)":107,"(A:34)->(T:02)":35,"(T:02)->(A:34)":85},"score":2180,"score_norm":0.954,"seq_a_single":21,"seq_ab_match":83,"seq_b_single":21}
ccgtgcctcattcggttggggcgaccaagaaaataatcattctttaaatataagattcacaaatcaaccaaattgatccttataaaagatcaccgaatcaagctaccttagggataacaggaggcacgg
+
CCCCCCCCCCCCCCCCCCCCCEEEEEEEECEEDDL`YY\Jc__\dadcdd`[cJaPcccaacc`_^]\RaEE?AEA=EEDEEEALEEBEDEEELEEEEEEEEEEBELECCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8673:9315#0/1 {"ali_dir":"left","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":54,"pairing_fast_overlap":74,"pairing_fast_score":0.761,"pairing_mismatches":{"(G:34)->(A:07)":46,"(T:02)->(A:34)":103,"(T:02)->(C:34)":97,"(T:02)->(G:34)":92},"score":2550,"score_norm":0.932,"seq_a_single":34,"seq_ab_match":69,"seq_b_single":34}
ccacagacgagcacggcatgtgccaaggaaaacaaaatatgagaaggacttgtcttgttatgccccgttttcggtgtgcatactggtcgtggcctcctttgaatcacaaacgactctcggcaacggatatctgcgtctgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCBYccVZS\\]V^G^RU\[cc\]aea^c[]_^b[Z\`_[]Z\ada=CCECEECEEBEECLELEELEEEEELEEEEECCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8662:18286#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":81,"pairing_fast_score":0.897,"pairing_mismatches":{"(C:02)->(A:33)":97,"(G:34)->(C:07)":48},"score":2328,"score_norm":0.975,"seq_a_single":27,"seq_ab_match":79,"seq_b_single":27}
cccgactagcaccgaaggcgtcaaggaacactgatattgccatgcgagggtgagggctagcttgctggcctaaccccaagcagcgatcaaccattaatccacacgactctcggcaacggatatctgctagtcggg
+
CCCCCCCCCCCCCCCCCCCCCBCCCCCcY__X_c][[[LU\RX`Uc_G_bbae^^WJ\IILBLLKMDJHGVZ_A?AB?EAEEEEBCCD=DDEEEE?KABDCCCDAEE?CBCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8700:12017#0/1 {"ali_dir":"left","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":93,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:02)->(G:34)":105,"(C:02)->(G:34)":93},"score":3997,"score_norm":0.968,"seq_a_single":15,"seq_ab_match":90,"seq_b_single":15}
ccagcgatcagccattgagtctctgcacctatccctttttttcttgctttcttcatcctttattttgcttttgaaaaaaaggaaaataaaaggagttggctcaggattgcccctgatcgctgg
+
CCCBBBCCCC?CCCC]U`]d^db_ac\]d^ebd_`O[`]_bY^^YMW\eee^a__Y\MH\dbdbYV[Z`baa^\dcceEEEBB?BEEEEEEELEEEEELEEEEELEEECCCC=CCCBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8661:3750#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(G:34)":102},"score":4365,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagagtacgcgggcaatcctgagccaaatccgtgttttgagaaaacaagtggttctcgaactagaatccaaaggaaaaggataggtgcagagactcaatggcagagctctgg
+
CCCCCRaVa]_^]Zda_cS`c^e]ceecaddceeeeeedee_deeeeeadce_edae`adea_eba_aEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:2659#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":100,"pairing_fast_score":0.835,"pairing_mismatches":{"(A:02)->(G:34)":105,"(A:06)->(G:34)":78,"(G:34)->(T:09)":22},"score":4106,"score_norm":0.96,"seq_a_single":8,"seq_ab_match":96,"seq_b_single":8}
ccagtcgagctgggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggagctcgactgg
+
CCCCCCCCEEEEEEEEa[[\ZFebLecccT_]eeeeeeeddbdddeddbdbdbeeeebed_Z_`^_\aaZeacceYcGcEEEEEEEEELEEEEEEEEEEEEEEELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8644:12753#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4774,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
cctgtacagccgtatatacatacgtatacgtcctgaaatactatctcaaaaaatgaatgacgacccgaatctttatttctttttttatatttatataaaaactgaatgaaa
+
CCCFEEEEEEEEEfbee_ceaWU[aaea]ab`aZ`\Xa]OVcdaeddeeefee^`b`]aa[WXY]^\\^Z\[a```c^\ac\c]cEEEEEEEEDDEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8665:13770#0/1 {"ali_dir":"right","ali_length":68,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":68,"pairing_fast_score":0.938,"pairing_mismatches":{"(A:34)->(G:02)":44},"score":3229,"score_norm":0.985,"seq_a_single":40,"seq_ab_match":67,"seq_b_single":40}
ccgatccccccgcctaccccacaccacgctcttcccatctcccacgtagctgatatccgttgccgagagtcagtggggtgttccttgacgccttcggagctacgtgggagatccgaagagcggttcagcaggaatgcggagaccgata
+
########################################EEELEEEEEEEEEEEEEEb_ddd`^T`Td`Xb^_Xb_bc_\ddc_cce\bb_cY^bb_\XYZUX_SW]AC:??/?A9AAACC;@########################
@HELIUM_000100422_612GNAAXX:7:1:9227:19549#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":3593,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCCLEEECa_YVcebeRQPPRSYWaUX\\YY\U[Y[^a^\[eac^c]bc^ce^e]eebacZbceebCCCCCCCCCBCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8691:9421#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5375,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccaacacacactaggattagataccctattattttagagcgttaaattttgtgcctgagtagtattagttatgttcttgagtgtgttggagatcggaagagcggtcca
+
<<:=:?=?=AAA>;;3<;C_eeed^d_cccee[aaac_cbeecbcc`ceeeeecdfefedeeeeeeeeeeeedac_cc_RRRRQRXXZZ_a__ceeedEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8923:15792#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tatctca:tatctca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatctca","obimultiplex_forward_tag":"tatctca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatctca","obimultiplex_reverse_tag":"tatctca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(G:33)->(A:02)":20,"(T:26)->(G:02)":39,"(T:34)->(A:02)":26},"score":404,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCBCCCACCCCCCDCCCCKDLEEELDBEEBEED@=@=D>5A;<;<==@=5=ZGJQQRdcaCEEEEEEEEEEEEEDLFEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8702:12758#0/1 {"ali_dir":"left","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":82,"pairing_fast_score":0.937,"pairing_mismatches":{"(G:33)->(T:08)":57,"(G:34)->(T:07)":56},"score":3718,"score_norm":0.976,"seq_a_single":26,"seq_ab_match":80,"seq_b_single":26}
cccatcagcagccgaaggcgtcaaggaacactcatattgccttgtccggtggagcggtcggcctgccttccgctccccgcgcagcgatgatatcttaatccacacgactctcggcaacggatatcctgctgatg
+
CCCCCCCCBCCCCCCCCCBCCCCCCCEEEdebac]`\[YIPJJI\Q_LaaaUa]QGEOHTHPaWZ_bacXdada]cbebeeZb^e^cefXcbb]cEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCB@C
@HELIUM_000100422_612GNAAXX:7:1:8701:11147#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":85,"pairing_fast_score":0.878,"pairing_mismatches":{"(A:02)->(C:34)":96,"(G:34)->(A:02)":35},"score":2780,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
ccatcgatcgcccgaaggcgtcaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatcgcgatcgatgg
+
CCCCCCCCCCCCCCCCCCCCCCCELEEEEFFEEELEDDbbcbXQd`c__]YTUUVSK]`cceY^[_`a`eecdbeeecH]^bEECEEEEDEDEEELEEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8625:17825#0/1 {"ali_dir":"right","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":74,"pairing_fast_score":1,"score":5125,"score_norm":1,"seq_a_single":34,"seq_ab_match":74,"seq_b_single":34}
ctacacgctttccctacacgacgctttccgatctgctcgatatccgttgccgagagtcgtatcgatggcggcgtgcctgtgttccttggcgtcatccgagcgctgtggagatcgcaagagcggtccagcagaaatgcggaga
+
################?=C=CCC?@CCCC@9ACCcabeceeeebfefeeeeeeeeebeecd_edcddcde\dedddaeededbc_e]cabd`b^WYU[\\ddcba^cE##################################
@HELIUM_000100422_612GNAAXX:7:1:9192:15735#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acggtac:cacacaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acggtac","obimultiplex_forward_tag":"acggtac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"cacacaa","obimultiplex_reverse_tag":"cacacaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"score":1427,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CBCCCCBCCCCCCBDCCDeeaed\a_`deeddd_[Z]`DDEEEDEECC?=CDABEBEB?CCDBDEEEEEECEEEEEEDEAECCCCCCBCCBCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8634:9708#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":84,"pairing_fast_score":0.938,"pairing_mismatches":{"(A:02)->(G:34)":108,"(G:34)->(T:02)":37},"score":2437,"score_norm":0.976,"seq_a_single":24,"seq_ab_match":82,"seq_b_single":24}
gcctacacgacgctcgtccgatctacgggcaatcctgagccaaatcctattttccgaaaacaaagtgaaaagggggataggtgcagagactcaatgggtgctctctggagatagaaggagcgtttcagagga
+
########################EBEEAEEEDEAELBEA`Rd\^\[`_bcaYe_\^^^cce[OZW`ZdbXbdddbQbEEEEEEEEEEEEEEEEDEEEEDEEEEEEEL########################
@HELIUM_000100422_612GNAAXX:7:1:8671:3540#0/1 {"ali_dir":"right","ali_length":67,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":67,"pairing_fast_score":1,"score":4673,"score_norm":1,"seq_a_single":41,"seq_ab_match":67,"seq_b_single":41}
cgagatctacactctttccctacacgacgctcttccgatctcccactagcgtgatatccgttgccgagagtcccactgtgttccttgacgccttcggacgctagtgggagatcggaagagcggttcagcagaaatgcggagacagatct
+
#################################@@@5@BBAbcbcdcbdbdcbddaddbaddddddbddc_dddcbdddddddadd``abYbc`bacbYd\][[T[_b??=78:6:8AA##############################
@HELIUM_000100422_612GNAAXX:7:1:8695:14214#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":103,"pairing_fast_score":0.92,"pairing_mismatches":{"(C:34)->(G:02)":10,"(T:34)->(A:02)":14},"score":3882,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
ccagctaggcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccgcactagctgg
+
CCCCCEEEELEEELEEEEEEEEEEEEEac_ccc\\\^FcI`ccde\bbdfacee`ed]edeeecdaeace]de^eceeeea\WaEEEEEEFEEEEEFEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8671:13946#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":104,"pairing_fast_overlap":107,"pairing_fast_score":1,"score":5025,"score_norm":1,"seq_a_single":1,"seq_ab_match":107,"seq_b_single":1}
cccagctgtgaccattgagtctctgcacctatcccttttttctcgctttctgaactctggtttgttcgcgtaaaccaggatttggctcaggattgccctcacagctggg
+
C^YY_`_a_QLNPM]bbM_c\aa_cc^cd_UY_Xbbbee_bebbe^eeecaWeededdcd`ebab_bee_S``aa[`dBDEEEEEEEEEEEEEEDEEEEEEEEEDEEEC
@HELIUM_000100422_612GNAAXX:7:1:8683:1477#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:34)->(G:20)":15},"score":4638,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccagcacagctccattgagtctctgcacctatccctttttgattttaactttctgaacttttatttgttttcagaaagaaggatttggctcaggattgcccagctgtgctgg
+
CCCCEEEEEEc_X\DaZcacc]d_bc\]__aae_e]eeeddZdceeebeeefeddZ`^`beedcabdaedbdbebcceEEEEEEEEEEEEEEEEEFEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8698:1585#0/1 {"ali_dir":"right","ali_length":88,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":88,"pairing_fast_score":1,"score":5138,"score_norm":1,"seq_a_single":20,"seq_ab_match":88,"seq_b_single":20}
acacgacgctcttccgatctaatatataaaatatttcatattttctataggaataatttcttatctaatattaaagataggtgcagagactcaatggactcgctctggagatcgaaagagcggttcag
+
########?4<6;'<7<:C@aeeebeedeeeeeeebeeeeeeecefdeeeeeeefceebeeeebeccaeeeecbbc_a\_dac_bbcc__cEEEEEEEEEEEEEEEEE####################
@HELIUM_000100422_612GNAAXX:7:1:8647:9837#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(C:34)":102},"score":5676,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagtacgctgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccagcgtactgg
+
CCCCCCEEEEcc_^bddaddbddbceeeeb_c\^eebeedeedeeedfee_deeefeeeeeceecddedeebdeaceeeebb_`__``cEEEEEEEEEEEELEEEEEEACCBCC
@HELIUM_000100422_612GNAAXX:7:1:8676:16458#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":4320,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
ccagagacagcccattgaagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccgcagatgtgg
+
CCCCCCCEEEEEEBEEEEcTZZ[XXZeeacbccWaebdeeededcceeee`c\cca]eeeec]dc``Zc_ac__cVXZZZdcEEEEEEEEEEEEEEAEEEEEEEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8614:20428#0/1_sub[28..127] {"ali_dir":"left","ali_length":66,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acggtac:)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acggtac","obimultiplex_forward_tag":"acggtac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_primer":"tagaacaggctcctctag","pairing_fast_count":54,"pairing_fast_overlap":66,"pairing_fast_score":0.857,"pairing_mismatches":{"(C:02)->(G:07)":56},"score":1015,"score_norm":0.955,"seq_a_single":42,"seq_ab_match":63,"seq_b_single":42}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
BCCCCCCAACCCCCDAEB`cPc^edc`[bcb`eD8>DB>CC,C5;158)-2,2=8186>ECAEEF?EEBECEEEEELDECJCCACCACCACCCA<BB;CC
@HELIUM_000100422_612GNAAXX:7:64:2567:18202#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":61,"pairing_fast_score":0.983,"pairing_mismatches":{"(T:34)->(A:02)":48},"score":3169,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
ccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggaagcctgttctactacttcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCBCCLcdeafS`ededebcddebe]Y\cbcc]cbXZb`c_]]a][[Z]]^aaDEEDEEEDEDEBECCCCCC@CCCCCC>CCCCABCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:28:15703:5948#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:34)":103,"(C:02)->(A:34)":85,"(G:02)->(C:34)":90},"score":1165,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggcagcctgttctagatattcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCDDCBCCCCEEFFc]Sc``ccab^^b]]W_[ECCDCBC@EC@DDEEELDEEELBEEEEEEEEEEELCEEEECBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8655:6260#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(C:02)->(A:34)":102},"score":4536,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccagcagatcggggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgatctgctgg
+
CCCCCCCCEbaM`]ZW]XMb]_\c_db`ee_ceeeccdebd]bbbeddddbddeeebaee_bb`_VXZZYcbbbc\aEEEEEEEEEEEEEEEEEEEEEEEELEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8671:7175#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":84,"pairing_fast_score":0.951,"pairing_mismatches":{"(C:10)->(T:34)":92},"score":5122,"score_norm":0.988,"seq_a_single":24,"seq_ab_match":83,"seq_b_single":24}
cccgacacgacgctcttccgatctccacagacaggtcgccccaaccgaatctttagtcaaggtgttgatttattgataaagctctatagggtcttcttgtgtctgtggagatcggaggagcggttcagcaga
+
##################A0@@C3^^V]eR_`^^_M\_`I`dX^eee^e`cdcdce`e^ZPZZWeebeeeeecc`_ccQQUZZXc_a\aRMFRRZZ\^^cbbb`[]^Q?#######################
@HELIUM_000100422_612GNAAXX:7:1:8634:1223#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":5714,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacacggcgctcttccgatctcactgcagtggggcaatcctgagccaaatcttattgtctattaaaaataagataggtgcagagactcaatggcactgcagtggagatcggaagagcggttcagaggaa
+
#################A=A:9==6\\cadaY]a[c_acb_edeebeeeeeeeZebeecffeeeeedddeeeeZ`d`e^edcbaeebeebcbee]_Rd_^Y^^[]\dSAB#######################
@HELIUM_000100422_612GNAAXX:7:1:8621:5122#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":81,"pairing_fast_score":0.744,"pairing_mismatches":{"(A:33)->(G:02)":42,"(A:34)->(C:02)":46,"(A:34)->(G:02)":37,"(C:31)->(A:02)":28,"(C:34)->(G:02)":47,"(G:31)->(A:02)":31,"(G:31)->(C:02)":43,"(T:31)->(A:02)":33,"(T:34)->(G:02)":40},"score":429,"score_norm":0.889,"seq_a_single":27,"seq_ab_match":72,"seq_b_single":27}
ccagactgctcggatgacgccaaggaacacgataaaagctgaggcaccggcgagccgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatcaagcagtctgg
+
CCCCCCCCCBCCCBB?CAACCCBCCCCIEEIAIEEELECL:KI9BLLAE.EEEEEbTYU_^FTOJ5B@AEEDEE>EDEDEEEE,EEEEAAEE@@@@@DEEEEEEEEBECCCCCCCCCC=CCCC@BCCBCCCCCBC
@HELIUM_000100422_612GNAAXX:7:1:8865:17473#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaggctt:gaggctt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaggctt","obimultiplex_forward_tag":"gaggctt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaggctt","obimultiplex_reverse_tag":"gaggctt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:23)->(a:02)":41},"score":1389,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCDCCCCCBCCBCLELEEEEEDEEEAEDDEDD@?AA=A9;;5>EED>ECC^W^__\Xcaa]R`aZa_HaU[^_EEECCCCBCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8657:13678#0/1 {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(T:02)->(A:34)":94},"score":2236,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
gatatcccccaacataccaaaactagctgtcactccaccagttgtcggagatgtaaggattgatacataaaataactttttatttaattgataatcatataaagcagacgatattttagccatttgcatcaagctcaaacttccttcctgcat
+
CCCCCCCCCCCCCBCCCCCCCCCCBCCCCCCCCCCCCCBC?ACCCQ^dac\\cZR\ZXZSZZa\^]_`^`ccecQINMWW]dFEEBECECEEELDEDEEEEEEEEEECCCDCCCCCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCCCCCCCCD
@HELIUM_000100422_612GNAAXX:7:1:8994:16234#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtaatta:gtaatta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtaatta","obimultiplex_forward_tag":"gtaatta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtaatta","obimultiplex_reverse_tag":"gtaatta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:33)->(G:02)":34,"(A:34)->(C:02)":49,"(C:32)->(A:02)":27,"(T:28)->(C:02)":36,"(T:34)->(A:02)":26},"score":337,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCCBCCCBCCBDEBECLJCEEEBEK@FBADD;DC>=A<ALB=BAKQ9MRebeCEEEEEEBEEEEEEELDEEECCCCCCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8660:10984#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(G:02)->(C:10)":95},"score":4106,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
catctccacgactctgccattgagtctctgcacctatcctttttttattttcactttctgaacttttatttgtttcaggatttggctcaggattcccccagagtcgtgggatc
+
#####EEEEEEEEEEEEEDEEaVcdaddddddddddcd\____]]b`bbc^cQ[^^[[XZRRPYZaaQ\^cUccVRU]\^baba[C<CAD=ADA4DDD>A=A>>>DDD#####
@HELIUM_000100422_612GNAAXX:7:1:9213:1890#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:26)->(A:28)":35},"score":1451,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaaatattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCBBCCCCCCAC?Ce_ebbcdS]__U`ec=Wdc^b^T`?DC?BBEEDEECDEEEEEEEEEEEEEEEEEDEELEEEKCBCCCCCDCCCCC?CCCCB
@HELIUM_000100422_612GNAAXX:7:1:9082:20958#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(c:19)->(a:34)":67},"score":2760,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCEEEEEEEEFa[LYW\RVZVYO]PJ]ac^\S\\ZVXVTWcaZ\Qca\\^D[cb^]D]UQ\]_DECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8636:7090#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:ttctaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttctaac","obimultiplex_reverse_tag":"ttctaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4484,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cctctaa
+
eeeeeee
@HELIUM_000100422_612GNAAXX:7:1:8644:16075#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":105,"pairing_fast_score":0.882,"pairing_mismatches":{"(C:02)->(A:34)":97,"(C:02)->(T:34)":101,"(C:33)->(A:08)":24,"(T:02)->(G:34)":100},"score":3960,"score_norm":0.962,"seq_a_single":3,"seq_ab_match":101,"seq_b_single":3}
cccgcacgtagggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaaagataggtgcagagactcaatggtacgtgcgtgg
+
BBBb_bb\^bbJMILIQKQPP[MER[_]X[WbSZbbdR\c\`db^____dd\Y`\d\aa^ddbdc`cc_ccDDDEEEEEEEEEEEEEEEEEEEEEELEELLEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8827:9261#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtgcctc:gtgcctc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtgcctc","obimultiplex_forward_tag":"gtgcctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgcctc","obimultiplex_reverse_tag":"gtgcctc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:33)->(g:02)":33,"(t:02)->(g:10)":71},"score":951,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaacgcaaaggacctggcggtgcttcatatccct
+
C@CCCCCCDCCCCCCCCCCEIEECELCLEEECKDaMOdZeb^QD@HMORQ@H_W_A0>><<<EBECE@<>4<ECEEE=EEBACCCCCCCCC?BCC?CCCC
@HELIUM_000100422_612GNAAXX:7:1:9219:4509#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agtgcaa:agtgcaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtgcaa","obimultiplex_forward_tag":"agtgcaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtgcaa","obimultiplex_reverse_tag":"agtgcaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":63,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:02)->(G:30)":47,"(C:02)->(T:34)":64},"score":1388,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
C@C8CAC==AA>=9CC@BOTXO]Pe\`U]Yc\SZ`LYZ^WZTacEEH9;<?EE=AEELEE?EELEEEEEEEEELELCEELECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8623:3365#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":92,"pairing_fast_score":0.764,"pairing_mismatches":{"(C:34)->(T:02)":17,"(G:34)->(A:07)":23,"(T:02)->(A:34)":73,"(T:02)->(C:34)":99,"(T:02)->(G:34)":103,"(T:34)->(G:06)":29},"score":3136,"score_norm":0.935,"seq_a_single":16,"seq_ab_match":86,"seq_b_single":16}
ccgatctacctgttatccctaaggtagcttggttcggtgatcatgtttaaggatctttgggttgacttgttaatctgtagatgataggatgatttttccttggtcgccccaaccgaatgattgg
+
CCDCCCCCCCCCCCCCLaa_]cGcaX][Gcbbccc`ca___cabeeeebe_`aceeeeecced`aDEEEEEEL@EFEEEEEEEEEEEEEEEEEEEEEELEEELEEEEECCCCCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8673:8893#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":96},"score":4881,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccacgtcgagtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccactcgacgtgg
+
CCCCCCEEEEdaZd\c_T^ebeeeXaYK]beeTeceaeeebefefeeeedccded`eeeeeebdddbeeeebdea_cc`cEEEEEEEEEEEEEEELEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9115:4408#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agtgcaa:agtgcaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtgcaa","obimultiplex_forward_tag":"agtgcaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtgcaa","obimultiplex_reverse_tag":"agtgcaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"score":1633,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCCDCCCBC@BBC_aU]ece`_ddbced]_]^c^^\aEEBED>>BBEEC5CEEEEDEEEEEEEE?DEEEELEEEFECCCCCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8693:2350#0/1 {"ali_dir":"right","ali_length":70,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":70,"pairing_fast_score":0.881,"pairing_mismatches":{"(A:34)->(C:02)":68,"(T:31)->(G:02)":59},"score":2410,"score_norm":0.971,"seq_a_single":38,"seq_ab_match":68,"seq_b_single":38}
gatctgcactcgttcccctcacgccgctcttccgttctcccagactgtcccgaaggcgtcaaggaacaccccagcgtagactctcggcaacggatatcgacagtctggagatcggaagagcggttcagcaggatgcggagagcgat
+
######################################EDEEEEEEEEEEEEEEEDEEIEEEEEEEELeabedRd`_b_[Pdb\dbUWXZ]S^__]Yeab_bUT^I^I==@;:C=C:A;@??########################
@HELIUM_000100422_612GNAAXX:7:106:14210:10196#0/1_sub[79..82] {"ali_dir":"right","ali_length":57,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gactcct:gcctcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gactcct","obimultiplex_forward_tag":"gactcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":57,"pairing_fast_score":0.944,"pairing_mismatches":{},"score":418,"score_norm":0.982,"seq_a_single":51,"seq_ab_match":56,"seq_b_single":51}
tttt
+
DDDB
@HELIUM_000100422_612GNAAXX:7:110:13075:18670#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(A:02)->(G:34)":103,"(C:26)->(G:02)":49,"(T:34)->(G:02)":47},"score":1063,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagccctgttctagatattcgg
+
CBCCCCCCCCCCCCCCCCDCCCBCCCCCCCDCCCCBCCCCCCCCCALEDC?`^cZ]]`Xa[]]XHXVX[A@@9=C5.CC=9,=>5>>=;CC7DCDBEEEBEELE?EEEBBA8BCCCCBCCC@CCACDCCCCCCBCCCCCDCBBCCBDCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8768:8206#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaggctt:gaggctt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaggctt","obimultiplex_forward_tag":"gaggctt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaggctt","obimultiplex_reverse_tag":"gaggctt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(t:02)->(c:33)":70,"(t:30)->(g:19)":41},"score":2260,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattctccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
ACCCDCCC?CCCCCCBCBCELELEEEEEcd]aTYLYZ_]_@\Q_UZ]XSX]]accU`WW^VVZbQ^cc?KBEEEEEFEECECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8907:20531#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgccgg:gcgccgg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgccgg","obimultiplex_forward_tag":"gcgccgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgccgg","obimultiplex_reverse_tag":"gcgccgg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(C:32)":72,"(C:02)->(G:30)":56,"(T:02)->(G:15)":35},"score":405,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaagtattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
B>B1?,???7<769:'74:4;=ZHUYJFW]a=?=9C??EEEEEEEDE?@?,AC=EH??E:EEBEEEEEEEEJEDLBLEE=LCBCCCCBCDDCCCBDCCCC
@HELIUM_000100422_612GNAAXX:7:1:8616:20127#0/1 {"ali_dir":"right","ali_length":50,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":47,"pairing_fast_overlap":50,"pairing_fast_score":1,"score":3199,"score_norm":1,"seq_a_single":58,"seq_ab_match":50,"seq_b_single":58}
aatgattccgcggacaccgatatctacactctttccctacacgacgctctgccgatctcctatctcaggtagtactctggcgaatagtggggtatctaatgagataggagatcggaagagcggttcagcaggaatgccgagacagatatcgtgtgctgtcttctgt
+
############################################A-??>B)7B@@CCBcdddd`c\a\UQTQVYSSXZaY__\bd^d`\V`VX^bd]a`d``adc`ccBB>B######################################################
@HELIUM_000100422_612GNAAXX:7:1:8668:11646#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":89,"pairing_fast_score":0.884,"pairing_mismatches":{"(G:32)->(T:02)":31,"(T:02)->(C:33)":99,"(T:34)->(G:02)":29},"score":2674,"score_norm":0.966,"seq_a_single":19,"seq_ab_match":86,"seq_b_single":19}
ccagtcgtcctgttatccctaaggtagcttggtctttgatctattgttaggatcttttgagcgattagataatcttatttgtggtggatgatgaattccatggtcgccccaaccgaatgacgactgg
+
CCCCCCC?CCCCCCCCCCCEEEEE?DEELEJ;ACDEEEEEa``aada^_UWSP_`]`[QLIZ[N\ZS[[]]SYW]U]`\SdddTbbD>?BDBD7DDDDKDDDDBDDDDBBBBBBBBBBB=BBBBBBB
@HELIUM_000100422_612GNAAXX:7:1:8685:10729#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4960,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccttcaacgggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaacgttgaaggagaccggaagagcggttc
+
<<0=?==?,8>9:+<ACC`^`aYebeeece][cbeeccWY\YXbcbdadeeebd`bbZefcdeeeeeebeeedede`ZYZZZVRRPQReeeZcECEEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8648:14968#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":89,"pairing_fast_score":0.942,"pairing_mismatches":{"(C:02)->(G:34)":108,"(C:02)->(T:34)":97},"score":4445,"score_norm":0.978,"seq_a_single":19,"seq_ab_match":87,"seq_b_single":19}
cacgacgctcctccgatctccgaggcttactaggattagataccctattattttggaatgtaaattgttttgcctgagtagtattagttatgttcttgaaagcctcggagatcgaaagagcgatcca
+
########?:'>2?;==A0JOQR\XVV]RRRRJ\W^a^\cb_ceead`cebeab^dcdbcb`db[_`_ceb^de_Ya_PRRJRRJXXXZc_`_caELEEEEEEEEEEL###################
@HELIUM_000100422_612GNAAXX:7:1:8694:6541#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":5165,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ccctcacgacgctttccgatctccaacctgacaagaagaccctatagagcttaatttaacctactaaataagtaaaaattaaattcggttggggcgacctcaggttggagatcggaggagcggttcagca
+
##########@A?5>9=3ACCCdddaccbecebeeeeebeeeeefdeeeefeeeeeeeeeeecceeceecee[_`_ceeb^eaa]a_X\^\XaWcEEEEEEEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8621:20906#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":51,"pairing_fast_overlap":85,"pairing_fast_score":0.622,"pairing_mismatches":{"(A:34)->(C:02)":30,"(A:34)->(G:02)":27,"(C:02)->(T:22)":94,"(G:25)->(T:02)":52,"(G:33)->(T:02)":26,"(G:34)->(C:02)":35,"(G:34)->(T:02)":61,"(T:02)->(A:32)":93,"(T:29)->(C:02)":59,"(T:34)->(C:02)":38},"score":531,"score_norm":0.871,"seq_a_single":23,"seq_ab_match":74,"seq_b_single":23}
cccacgcagtcccgaaggcgtcaaggaacactgtgcctaacccgggggcatgtctagcttgctagccgtccctcgtgttgcaaagctatttaatccacacgactctcggcaacggatatcgactgcgtggg
+
CCCCCCCC@>CCCCCBCC@C@CCEEKLEELEEABLEBLEBEEEDDECBE@<C=BE;LAGDL7>BCEQVLV__UXXYXH<>=>EEAE>EECEEJ@EEEEEEEDEDEEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8623:7836#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":89,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(G:34)":87,"(C:02)->(T:34)":97,"(G:29)->(T:22)":36,"(G:34)->(T:02)":23,"(T:02)->(C:34)":99,"(T:02)->(G:33)":96,"(T:02)->(G:34)":103},"score":2553,"score_norm":0.921,"seq_a_single":19,"seq_ab_match":82,"seq_b_single":19}
ccgaatatcctgttatccctaaggtagctttatttggtgatcttatttaagggtctttgtatgacttaatggtctttgtaataaaggatgatgtggtccttggtcgccccaaccgaatgatattcgg
+
CCCCCCCCCCCCBCCCCCCEEELEEEEEEEDEEE^>`cSO``c_ccccQ]RXcVadeeebe]_]ZeeeabS\\\^aEDDAEDEBEELCEDEEEBEKLELEEELEEEEECCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:9226:2921#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agtcgtc:agtcgtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtcgtc","obimultiplex_forward_tag":"agtcgtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtcgtc","obimultiplex_reverse_tag":"agtcgtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{},"score":1509,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCC=BBBBCCCCCCCCCCLELDEEEEMCEEEADDEDD8C@CCC><A0>B?ABE=@\VG]VT[__cbdbe[\cQYRJZ\]\`CC@CCC<CCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8635:13321#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":6766,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccagcactctcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggagagtgctgg
+
CCCCCC]ce_cda]b\e^bedXececeeeeeeeed`__]eeX`aeeecbdeeeebeef_deeZadaeeeabccad_eeecc\cc_XRWZXRRQQQS`R]Y`__Y`bcaCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:16948#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":85,"pairing_fast_score":0.707,"pairing_mismatches":{"(A:34)->(C:02)":27,"(A:34)->(G:02)":48,"(G:31)->(C:02)":65,"(G:33)->(T:02)":35,"(T:34)->(C:02)":59},"score":2646,"score_norm":0.929,"seq_a_single":23,"seq_ab_match":79,"seq_b_single":23}
cccactacgtgccgaaggcgtcaaggaacactatgcctaatgcggggacacggctggcttgctagccgtccctcatgttgcaatgctatttaatccacacgactctcggcaacggatatccacgtagtggg
+
CCCCCCCCCCCCCCCCCCCCCCCEEELEEEDEEEKCEEELEDEEDEDLFBEBADDFE@LEAEEEIEdbb]eeaZ^ebaaZd`Oe]_a`_c_cXWZS\a^aR`_\a^U]CCCCCC@CCCCCCCCCCCBCCCC
@HELIUM_000100422_612GNAAXX:7:1:8653:5767#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":102,"pairing_fast_score":0.879,"pairing_mismatches":{"(A:02)->(G:31)":87,"(A:33)->(G:02)":16,"(C:02)->(G:34)":103},"score":2207,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccacgactctggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaagaaaaaggataggtgcagagactcaatggctctctcgtgg
+
BBBBBBBDDD@@@@AKDDDDDDDBDDDDDDDa^bOa\a^S`]\db]ccZV^_X_S[\VSRYI\\\WaEDBBBBBEEEEEEEEEEEEIEEDEEEEEEEEEEEELA<?EECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:7929#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:34)->(G:31)":15},"score":5355,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagagagtcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgagcagtctgg
+
CCCCCCEEEEc`__Cbceecc`ccK\^ZYceccebeeecebedeeeedfdecddecfedeeeeeddeeeeeddee_WddddbbZ_a_cEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9334:2459#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:aacgagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgagc","obimultiplex_reverse_tag":"aacgagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{},"score":3152,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCBCCCCDCCCCLEEEEEEELe_bdU\YZXPTY[HXWWZMYXVZU`[NRWWIaa`\_^eccdeebebecSc_W`\CCCDCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8687:12697#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5151,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccgaagtagactaggattagataccctattattttggagcgttaaattttgtgcctgagtagtattagttatgttcttgactacttcggagatcggaggagcgttcag
+
<0:3>A;AAA@B8<::BA=Yac_\[Y][_c]c[]ZUYad_ebcddd][de_ebddbebeee_eeeeeeeeebb]dcca]RRRRQXXXYWdcdcbebZeEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8614:8120#0/1 {"ali_dir":"left","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":84,"pairing_fast_score":0.988,"pairing_mismatches":{"(G:34)->(A:02)":25},"score":301,"score_norm":0.988,"seq_a_single":24,"seq_ab_match":83,"seq_b_single":24}
ccagtgcacacccgaaggcgtcaaggaacactgtgcctaatccgggggcgtggctaagcttgcttgctgccccccgttttgcaatgctattcaattgacacgactctcggcaacggatatcgtgtgcactgg
+
CCCCCCCCCCCCCCCCCCACCCCCLDEEEEEAEEECDDECEEAECEEECD6EA0A==;=<??46?587^[RcEEEEEDCD;EEE@=EDD?EEEE@EEEBEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8616:12725#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:34)->(G:10)":16},"score":4840,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagcgctacggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgtagcgctgg
+
CCCCCBEEEEEa]]cFeV`^_^[_Wb[eccbacc_QPaa]_O`_eeeecaecbecddeecdedSb]eeecece_ebc_daeaaeEEEEEEEEEEEEEEEEEECEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8635:13769#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":7230,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacacgcagagggcaatcctgagccaaatcacgttttccgaaaacaaacaaagattcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggtctgcgtgtgg
+
CCA][\cUccea`KdaXVAUUebbdecbcZaeaececeb]]ddeebdbb_eeb`de_eeeeeeeceeeedeeeeeeee_eeedcc_\_`aeeeeVeeebbaee`eY\\CCC
@HELIUM_000100422_612GNAAXX:7:1:8624:6583#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":101,"pairing_fast_score":0.908,"pairing_mismatches":{"(C:02)->(G:34)":85,"(C:34)->(A:02)":9,"(T:34)->(G:02)":12},"score":4572,"score_norm":0.97,"seq_a_single":7,"seq_ab_match":98,"seq_b_single":7}
gctagctgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccctttctttgttttctcaaaacacggatttggctcaggattgccccagctagctgg
+
CCCCCCCELEELEEEEEccc__c__c_c]\ebeeedebeecccebeYdceee]aeeedbdc_cddb\`dacd`dd`abccccEELEEEEEEEEEEEEEEEEEEEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8701:4202#0/1 {"ali_dir":"left","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":91,"pairing_fast_score":0.909,"pairing_mismatches":{"(C:02)->(G:34)":90,"(T:02)->(C:34)":101},"score":2682,"score_norm":0.978,"seq_a_single":17,"seq_ab_match":89,"seq_b_single":17}
gcgtagatatccgttgccgagagtcgtgtggattaaatagctttgcagcacggggggcggcaagcaagccaaccgcgtccccgggctaggcacagtgttccttgacgccttcggtacgctgctgg
+
CCCCCCCCCCCCCCCCCeZeceace^bcbL`aWdc`_a]KV]b_^VWGTZ__b\cedabECEEEEEEBEEEEEEEEEEEDEDEEEEEEELEEEEEEEEEELECEEDEECCCBCCBCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8681:20767#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":89,"pairing_fast_score":0.965,"pairing_mismatches":{"(G:02)->(T:34)":106},"score":5154,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
cacgacgctcttccgatctccaacacacactaggattagataccctattattttagagcgttaaattttgtgcctgagtagtattagttatgttcttgagtgtgttggagatcggaagagcggttca
+
<;63@@BBAA1A=>A@CCA^c``c_aac]a\\Q_aSaadcdfceeedddeeeeccedeebddeeeeeeeeeebb___aYRROQQ\^\\^XLYYZc]ccEEEEEEELEE###################
@HELIUM_000100422_612GNAAXX:7:1:8638:5351#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":86,"pairing_fast_score":0.952,"pairing_mismatches":{"(A:02)->(C:34)":99},"score":3822,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ctacacgacgctcttccgatctccgcgcgaacaagaagaccctatagagcttaatttaacctactaaataagtaaaaattaaattcggttggggcgaccttcgcgcggagatcggacgagcggttcagca
+
##############?;?2?:<6^QQQQJVW__XcccbbeeZeeeeeeebededeeeceedc`_]eeeedeee_eeee`cEDEEEEEEEDEEEEEEEBELEDEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8647:2586#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":93,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:33)->(G:02)":32,"(A:34)->(G:02)":42,"(T:21)->(C:08)":78},"score":2426,"score_norm":0.968,"seq_a_single":15,"seq_ab_match":90,"seq_b_single":15}
tcgctcttccgagcgccagacgagaggggcaatcctgagccaaatcctgctttccaaaaaggaagaataaaaaaggataggtgcagagactcaatggctctcgtctggagacaggaagagcgg
+
###############EEEEEEEDEEEEBEEEKEEEEEDEDELEEEEEECaabT^a__^bbX_[b[VV`YU[UMPXPJ9RQKOVSP\[[QVJWYBBB=BBB=BBBBBBB###############
@HELIUM_000100422_612GNAAXX:7:1:8701:5290#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":105,"pairing_fast_score":0.549,"pairing_mismatches":{"(A:02)->(G:06)":77,"(A:02)->(G:14)":84,"(A:27)->(G:02)":6,"(C:02)->(T:15)":85,"(C:02)->(T:34)":98,"(C:07)->(A:34)":63,"(C:08)->(T:31)":38,"(C:28)->(G:02)":25,"(G:02)->(A:06)":78,"(G:02)->(C:11)":105,"(G:24)->(T:22)":54,"(G:26)->(A:02)":14,"(T:02)->(G:34)":100,"(T:25)->(G:25)":35},"score":1564,"score_norm":0.867,"seq_a_single":3,"seq_ab_match":91,"seq_b_single":3}
ccacgacgcgagggcaatcctgagccaaatcacgktttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaagagataggtgcagagactcaatggtcgcctcgtgg
+
?==@CECDDDB>?DDCECE:EBEDFCECE4Ea_aC[QC^aZ\?ea`YL\\[]W9:E[MP\]]G^bKKKP22(111*0001210892EEEEDECBBDEL7LEEBB5<DEC?C
@HELIUM_000100422_612GNAAXX:7:1:8627:15178#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":102,"pairing_fast_score":0.949,"pairing_mismatches":{"(C:02)->(T:34)":108,"(G:29)->(T:08)":42},"score":3513,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccatcgatcgcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggcgatcgatgg
+
CCCCCCEEEEEEEEcV__^eXd\^caRa^TbcecYKWYPOTAUFd_eX[baac\[ede]eeeac_Za\cc^aEDEEEEEEEEC@EEEEEEEEEEEEEEEEEEEEEEELCCDCCC
@HELIUM_000100422_612GNAAXX:7:1:9320:11326#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agcagag:agcagag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agcagag","obimultiplex_forward_tag":"agcagag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agcagag","obimultiplex_reverse_tag":"agcagag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3737,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCEEEd\_dd_adadXYJRZc[^d\[Y][YYU[[Vcae_e_cececb]edeacYc_Y[]TY^\[CCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8661:1854#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(T:34)->(G:06)":28},"score":5947,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccaacaacattagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgttgttggagatcggacgagcgcttc
+
################A=_dRbb\bbbG]]XVZTcceeZeb_ebdbeecaea\_`cc]addba_ece[Wedb]eeeaa_`beb_a[\`abZa]b^b`baec_b__Va_=C<)@#############
@HELIUM_000100422_612GNAAXX:7:1:9312:3254#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgatga:gccggcg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgatga","obimultiplex_forward_tag":"gcgatga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccggcg","obimultiplex_reverse_tag":"gccggcg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":58,"pairing_fast_score":0.909,"pairing_mismatches":{},"score":2344,"score_norm":0.966,"seq_a_single":50,"seq_ab_match":56,"seq_b_single":50}
tttt
+
ETb_
@HELIUM_000100422_612GNAAXX:7:1:9156:11521#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ggaggaa:agtcgtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggaggaa","obimultiplex_forward_tag":"ggaggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":1,"obimultiplex_reverse_match":"tagaacaggctcctgtag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtcgtc","obimultiplex_reverse_tag":"agtcgtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:34)->(t:02)":35,"(c:22)->(a:34)":73,"(c:32)->(g:02)":41},"score":1186,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCEELEEEEEEEEECEBDLDDBCEJE=<<>6AEEEBE?<??1C??_\acdebcb]_Ca]X[^`Y_CCCCCCCCCCCCCCCCCB
@HELIUM_000100422_612GNAAXX:7:1:8680:15679#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":4859,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccatcgtgcgtgggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggacgcacgatgg
+
CCCCCCEEEEEEDEEEEEEcU`cSNbe_ecebbeeeac_eeddaeeeba_deeeeddecaeeaaecebdcedecebeceac__d_a]aEEEEEEEEEEEEEEEEEEEECDCCCC
@HELIUM_000100422_612GNAAXX:7:1:8702:20007#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":3942,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccgatctaggcctcaagaacataactaatactactcaggcacaaaaattaacatcccaaaataatagggtatctaatcctagtggccttcggagatcggaagagcggttcagcag
+
#######################EEEEaceacbcc`[`accb_a^^^^_c_cb]ebce_ddd_deeeeeedcddeeeec[]_cEEEEEEEEEEEFEEEEEEEEEEEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:8698:9805#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":93,"pairing_fast_score":0.956,"pairing_mismatches":{"(G:34)->(T:18)":37},"score":4941,"score_norm":0.989,"seq_a_single":15,"seq_ab_match":92,"seq_b_single":15}
acgctcttccgatctccagagctctggggcaatcctgagccaaatcctattttccgaaaacaaagtgaaaagggggataggtgcagagactcaatggcagagctctggagatcggaagagcgg
+
==8B=BAA/<??<;/IXXeddceeeeeeZ^W^QWa_D_eeaWeWeeeeededeeedadeeeebbd[^]aXadadc]\]a]K\][U\]Y[Uc^aEEEEEDEEEEEEEEE###############
@HELIUM_000100422_612GNAAXX:7:1:9359:10073#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acggtac:acggtac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acggtac","obimultiplex_forward_tag":"acggtac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acggtac","obimultiplex_reverse_tag":"acggtac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:02)->(g:34)":77},"score":2052,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCDCCC@CELEEEDEEEccccRPIPRUV\aIJKPQPLQWDQYZ]RZWZ_YVU^\a^aDEEEEEEELEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8614:1271#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":101,"pairing_fast_score":0.929,"pairing_mismatches":{"(C:02)->(A:34)":101,"(C:02)->(G:34)":104},"score":5019,"score_norm":0.98,"seq_a_single":7,"seq_ab_match":99,"seq_b_single":7}
ccagagctctggggcaatcctgagccaaatccggttcatggagacaatagtttcttcttttattctcctaagataggaaggggataggtgcagagactcaatggcagagctctgg
+
CCCCCCCX_^^[T^c`baZaacdSb_bdab_cb__abee`e`edec]eeebeedbed_ebddc_d^eeca`aUa_YYYUYdaEEEEEEEEEEEEEEEEEELEELEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8644:6186#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5106,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccaattaacactaggattagataccctattattttggagcgttaaattttgtgcctgagtagtattagttatgttcttgagttaattggagatcggaggagcgtttca
+
########?;;:9:77:<=Kc_cKaa_accdeaRRRQQccecebdeddeeeeeddeeafccceeeeeeeeedeX^^Ua]RRRRQZ\^\\ccc`cecacEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8702:18609#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":89,"pairing_fast_score":0.953,"pairing_mismatches":{"(G:35)->(C:06)":33},"score":4847,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
cacgacgctcttccgatctccgaagtagactaggattagataccctattattttggagcgttaaattttgtgcctgagtagtattagttatgttcttgactacttcggagatcggaagagcggttca
+
CCA;;A>A@CCCA=8=@AA\__c\[[[^]U]aHRSRQQZbdcaeecdddeeeeddeeceeeeeeeeeeeeecc^a_\a[RRRRQX[[[[dccbcccEEEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:9128:2383#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agtgcaa:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtgcaa","obimultiplex_forward_tag":"agtgcaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:02)->(G:14)":51},"score":1575,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactgctagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCDBCCCDCCCBCEEEb_ccc^ebaeed[`d[X[\^JJOPLCP0*8898<7BEEABEELAEEEEBEEEEELAEELECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:12331#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":5510,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacacgactctcttccgatctccagatcgacgccattgagtctctgcacctatccctcgggcaccacagccttgtgttggctcaggattgccccgtcgatctggagatcggaagagcggttcagcagga
+
###################?=/=BAcdbaWRZWaeZbedbeeebeeeedef`eeeebdceXded`eeeeeeecedcedebedc]bebecRccb`_c]e\\^\RLXcEE#########################
@HELIUM_000100422_612GNAAXX:7:1:8618:2641#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":90,"pairing_fast_score":0.908,"pairing_mismatches":{"(A:34)->(G:02)":32,"(A:34)->(G:07)":76},"score":3473,"score_norm":0.978,"seq_a_single":18,"seq_ab_match":88,"seq_b_single":18}
acgccgctcctccgatctccagagtagcgccattgagtctctgcacctatcacgatatcgagcagatcatgcaacacctcttggctcaggattgccccgctactctggagatcggacgagcggttc
+
##################EEEEEEEEEEEEELEEEEEEEEEEEEEEEEEa_[]b_eded]S^^[cac_\a\cbceG_UU]`V^I`[a^RKRRRa[a]`c]eTa^X^\K@AA###############
@HELIUM_000100422_612GNAAXX:7:86:3316:15662#0/1_sub[29..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaatatc:aatatct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":1,"obimultiplex_reverse_match":"aagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aatatct","obimultiplex_reverse_tag":"aatatct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(t:27)->(g:34)":76},"score":3564,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacattcct
+
CCCCCCCCCCCCCCCC?ACLEEEd]caebaeb_^`YK^cd\c^a`^M[PYSPT\Z_cceec]`ac]YaXS^abeeCV_c^cCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8703:2996#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(A:34)->(G:02)":16},"score":4454,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
gccgatctcccactgactcgggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatgggagtcagtgggagatcgga
+
########EEEEEEELEEEEEEEEbP_W]`YaYUTTYN^eeb^ebeeeaeeddeeeeeeadXe`c^`ddbS^__c\\cccW\c[]a^abebaEBEEEDCCCECECEEE########
@HELIUM_000100422_612GNAAXX:7:1:8682:2157#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":54,"pairing_fast_overlap":73,"pairing_fast_score":0.771,"pairing_mismatches":{"(C:02)->(T:34)":93,"(G:02)->(T:34)":100,"(T:02)->(A:34)":104},"score":826,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacgcgatcggcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtgttgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatccgatcgcgtgg
+
CCCCCCCCCCADCCCCCCCCCCDCCCCC=CCDAAC\QYe`aVa\YVPPIS?B,1?C8CEB>ECA5CDEBEFDE@CDCB@EAELEDEEEEEEELEEEBEELEEELEEDECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:19274#0/1 {"ali_dir":"right","ali_length":65,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":36,"pairing_fast_overlap":65,"pairing_fast_score":0.581,"pairing_mismatches":{"(A:02)->(C:34)":93,"(C:02)->(A:34)":101,"(C:02)->(T:32)":100,"(C:02)->(T:34)":106,"(T:21)->(G:08)":73,"(T:31)->(G:20)":63},"score":1756,"score_norm":0.877,"seq_a_single":43,"seq_ab_match":57,"seq_b_single":43}
agcgagatgtgcgcgctttccctacacgaggctcttccgatctcgacgtgtacggatatccgttgccgagagtcccacgtgttccttggcgtcatcccgtacacgtggagcccccaagccagctcaacagggaccgcggcaccacagccgg
+
##########################?:93:3<+??/82)===aSZa`Z^>V]WaS^[WYYYAZa[b]\ZU[9bbIbEELEEEEEEEEEEDDLELBEBEJLEEDELEE###########################################
@HELIUM_000100422_612GNAAXX:7:1:8612:10698#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(A:02)->(G:34)":81},"score":4932,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccacgagagagctattgagtctctgcacctatcctttttgattttcgctttctgaacctttgtttgttttctgaaaccaggatttggctcaggattgcccctctctcgtgg
+
CACede\a_aaaUdc`_Zeeeaa\\_aX]^]eXbbbeecMeceeecefeedcdcbac\ccedbb`ddedd]^^aaEEEEELEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:9371:6268#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtgacgt:gtgacgt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtgacgt","obimultiplex_forward_tag":"gtgacgt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgacgt","obimultiplex_reverse_tag":"gtgacgt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(G:34)":56},"score":1660,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCC@CCCCCCCCD<Bd`cad`dY[ddbUeT<Sa\KTXSQacaaDDEDEEEDLAAAEDBECEFEEEEEECEEELECDLCCCCCCCCCCCCCACCCCB
@HELIUM_000100422_612GNAAXX:7:1:8622:12993#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":108,"pairing_fast_score":0.952,"pairing_mismatches":{"(A:02)->(G:34)":108},"score":4793,"score_norm":0.981,"seq_a_single":0,"seq_ab_match":106,"seq_b_single":0}
ccagatcgcgagggcaatcctgagccaaatccggttttctgacaataacaagttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggtcgcgatctgg
+
EEd\`acc\YaK__c\_YTa_]e]\eeec]_cN[[V]abZ[dbdeebeedcebe\cd_d^bc`]cddcU_aca_cEEEEELEEEEEEEEEEEEEEEEEEEEEEEEEEL
@HELIUM_000100422_612GNAAXX:7:1:8786:3974#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aacctga:aacctga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacctga","obimultiplex_forward_tag":"aacctga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacctga","obimultiplex_reverse_tag":"aacctga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3832,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
BCCCCCCCCCCCDCCCBCC_X]dcdZZVeaecbbW[cbL[X=ceeeaWTXTYXKZ[YY\cca]Se_beab]db`XXK]eebCCCCCCCCCCCCCCCCACC
@HELIUM_000100422_612GNAAXX:7:1:8703:13460#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":90,"pairing_fast_score":0.655,"pairing_mismatches":{"(A:02)->(T:34)":78,"(A:34)->(G:29)":38,"(G:02)->(T:31)":93,"(G:02)->(T:34)":104,"(G:34)->(T:02)":25,"(T:02)->(A:34)":89,"(T:02)->(G:34)":106,"(T:34)->(G:02)":24},"score":2298,"score_norm":0.9,"seq_a_single":18,"seq_ab_match":81,"seq_b_single":18}
cctggtggcatttcaggtcaaggtgcagcttgtttctaagtgtatgatggattacattgttatttgtttatttgaattgttggttttaatattgacatgaaattggatttggttgtagccaccagg
+
CCCACCCCCCCCCDCCCCDEEEELLEEEFDEaYPb_ZCaa`aa]\_]NR_`Na^ZXZeXWc^Vcc^^c]bWaEEDEBLEEEELEEEEBLABEIE9BEEEEEEALDLDECCCCCC0CBCCC?CCBCC
@HELIUM_000100422_612GNAAXX:7:1:8637:9570#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":103,"pairing_fast_score":0.83,"pairing_mismatches":{"(A:02)->(C:34)":97,"(A:02)->(G:34)":102,"(A:34)->(G:21)":66,"(C:02)->(A:34)":99,"(C:34)->(G:02)":25},"score":3100,"score_norm":0.951,"seq_a_single":5,"seq_ab_match":98,"seq_b_single":5}
ccagagcgagtgggcaatcctgagccaaatctttatttttagaaaaacaagggtttaaaaactagactagaataaaaagggataggtgcagagactcaatggactcgctctgg
+
CCCCCEEEEEEEEEEEEEAEEEDELebebeb`d`e`e^`ZPOS]cb^RG_`ec\b[_cQ]bYX`]Dab_dddeceEEEBEEEEEEEEEEFEEDEEELBLEELEDEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8958:3395#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (accaatc:aacggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"accaatc","obimultiplex_forward_tag":"accaatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacggaa","obimultiplex_reverse_tag":"aacggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3470,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCA@CCCCBCCCCCCCC=CYRT_^T_\bbc`^]^NY_ZZ`_`]_`]][Y_X]cd`]c^_aQ_`cbcee_eb]eEEEEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9113:16252#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gatgacg:gatgacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatgacg","obimultiplex_forward_tag":"gatgacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatgacg","obimultiplex_reverse_tag":"gatgacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(a:02)->(t:28)":48,"(c:02)->(a:24)":49,"(c:02)->(a:34)":59,"(c:02)->(g:32)":62,"(g:02)->(t:34)":81},"score":508,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
B<6BAB?8?A<94;>>>8B]O\\]_\_ZCCC@>=/5ACDEDE>>A<=FBA@7@>>>>2LCEJEECCCDCD?CEDEEEEEELCCCCCCCACCC@C=CACBC
@HELIUM_000100422_612GNAAXX:7:1:9196:8076#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tggtcct:tggtcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggtcct","obimultiplex_forward_tag":"tggtcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtcct","obimultiplex_reverse_tag":"tggtcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(c:02)->(t:34)":81},"score":3122,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC@CZD]\`a__SceeeRPHMR\ZO`UY]W[[XV[YX[Z_[\^G\SK[^Ua^^__U__cXaEEEELCCCCCCCBCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8615:18128#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":102,"pairing_fast_score":0.889,"pairing_mismatches":{"(A:02)->(G:33)":96,"(C:02)->(A:21)":79,"(G:02)->(T:33)":99},"score":637,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagctcagtgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccactgagctgg
+
CCCCC?EBA>@EBEEEEEEEEEEEEEEEEEEEEEEDEEB5A:C9A@B9CB@A:0@E@@:UK`ZPBY^Uc]9DAD?DD??<DDDDDDDDDDDDDDDKDDK@@@@<7<<>:BBBBB
@HELIUM_000100422_612GNAAXX:7:25:12238:2366#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":61,"pairing_fast_score":0.983,"pairing_mismatches":{"(C:02)->(T:34)":108},"score":1824,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagccctgttctagttaattgg
+
CCCCDCCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCCCCBCCCCCCCSa`c\]^^eeb\d]``eUe`a`e`eb]BD4BCDBEBEBCD=DBEEEEE@EBDEEEEE=EELCCCCCCCCCCCCCBCCCCCCCCCCCCCCCDCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9117:13290#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tatctca:gccggcg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatctca","obimultiplex_forward_tag":"tatctca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccggcg","obimultiplex_reverse_tag":"gccggcg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4087,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
ggcct
+
^\Zbe
@HELIUM_000100422_612GNAAXX:7:1:8703:11527#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(C:02)->(A:33)":100,"(C:31)->(A:02)":15},"score":3028,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccatgtcagcggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgctgacatgg
+
CCC@C@EEBE@A>>IEEEEEEEEEDEEBBb\_b_bZVc_[[TX]`S\ZP]Oce\bd]b`[`_``URXQV\d`aX_\T_>EAEEEDBEBEBEEBEAEEEAKEEEEDAEE>CCCCC
@HELIUM_000100422_612GNAAXX:7:1:9232:19330#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gccgtgc:gccgtgc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccgtgc","obimultiplex_forward_tag":"gccgtgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccgtgc","obimultiplex_reverse_tag":"gccgtgc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(A:02)->(C:19)":54,"(A:30)->(G:25)":32,"(C:02)->(G:19)":56,"(G:32)->(A:02)":20},"score":677,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCC?C?CCCCCCCCCCCCAJAAcNQL^W^XZ?UWRX\I:9*5DC>B?222203=1=36@CC,EEE?EE@CAEELEELDE=LCCCCCCBCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:9054:8280#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgagt:ttcgagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgagt","obimultiplex_forward_tag":"ttcgagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgagt","obimultiplex_reverse_tag":"ttcgagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(T:27)->(G:07)":36},"score":1268,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCCCbM]dbcdbTW]VOPF@@NWS`[\_DCDCE>E=DEEEEE>EEEEECEEEEEEEEEBEEEEEBFCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:19316#0/1 {"ali_dir":"right","ali_length":65,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":65,"pairing_fast_score":0.935,"pairing_mismatches":{"(T:34)->(G:10)":50},"score":4132,"score_norm":0.985,"seq_a_single":43,"seq_ab_match":64,"seq_b_single":43}
accgagatcttcactctttccctacacggcgctcttccgttctcccacgtagctccgaaggcgtcaaggaacaccccactgactctcggcaacggatatcagctacggagatcggaagagcggttcagcaggaatgccgagaccgatctcg
+
#################################?69;?5)(16_ac]aQFRQO][a^Tccccc`aZ`a_cccS^]^^Z`]aa\]edeeeebeefdeedcUbc_bcec]@CA<8==A8ACC;CC?=?#########################
@HELIUM_000100422_612GNAAXX:7:1:8617:19945#0/1 {"ali_dir":"right","ali_length":65,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":65,"pairing_fast_score":1,"score":4778,"score_norm":1,"seq_a_single":43,"seq_ab_match":65,"seq_b_single":43}
accgagatctacactctttccctacacgacgctcttccgatctcctaagtctctgttatccctaaggtagcttccgcgatgaggtcgccccaaccgaatagacttaggagatcggaagagcggttcagcaggaatgcggagaccgatatcg
+
#######?2(5A@AA;:8A>=A6=A=A=AC(A@C?C8C:@CCCeeeefeeeeedeefeeeeedeeeeebdedeeeeedfeeeeeecedeeeeeebeeeebeebcaeebCCB@;9:<'<???##############################
@HELIUM_000100422_612GNAAXX:7:1:8693:5782#0/1 {"ali_dir":"right","ali_length":54,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":51,"pairing_fast_overlap":54,"pairing_fast_score":1,"score":3929,"score_norm":1,"seq_a_single":54,"seq_ab_match":54,"seq_b_single":54}
atacggccaccaccgagatctacactctttccctacacgacgctcttccgatcttccgttgccgagagtccgaacggggtgttccttggcgtcatcccgatcgcgtggagatcggaagagcggttcagcaggaatgcggagaccgatctcgtatgccgtctt
+
############################?76<;64:5849=A?CC:CAC;CCCBceeeeeefeeedbeeeeeeeeeeeefeedeceeedfeeb]`eccecZaebededBCB@@CBC@AB==>B#######################################
@HELIUM_000100422_612GNAAXX:7:1:8680:3693#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":86,"pairing_fast_score":0.675,"pairing_mismatches":{"(A:25)->(G:02)":56,"(A:34)->(G:02)":26,"(A:34)->(T:02)":34,"(C:13)->(G:34)":86,"(C:34)->(A:02)":31,"(G:33)->(A:02)":25,"(G:33)->(C:02)":48,"(G:34)->(T:02)":27,"(T:32)->(C:02)":51},"score":3083,"score_norm":0.884,"seq_a_single":22,"seq_ab_match":76,"seq_b_single":22}
ccagactgctcgatatccgttgccgagagtcatatttttttctgttggcctcggcagccacagagccctcgagcggcccgccggcgcctcagcttttatcgtgttccttggcgtcatcccacgcgtgtgg
+
CCBDCCCCCCCCCCCCCCDCCCEEKLLEEELLELDEEEDDDD@DEEEKDEJCEEAC[VVZX\[[Wdcd[ceeecaeeeeeefed]ETWYU^b]_^X\XYQee`dW[_cCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8677:20436#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":105,"pairing_fast_overlap":108,"pairing_fast_score":1,"score":3009,"score_norm":1,"seq_a_single":0,"seq_ab_match":108,"seq_b_single":0}
gcagggcaatcctgagccaaatcctgctttacgaaaacaagggaaagttcagttaagaaagcgacgagaaaaatggataggtgcagagactcaatggtgctgtcgtgg
+
EEEEEEEEEEEEEEEEEEEEEEEEEEEEc_UcSdeeeed`d_deedfdfa\a_`_`eacac_bb^WX[YWebEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:8786:15430#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acacgta:acacgta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acacgta","obimultiplex_forward_tag":"acacgta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacgta","obimultiplex_reverse_tag":"acacgta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3454,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCDCCCCCCCCCCDCCCCEF`\aaPOPcJ`aWTSTW^XZ]MVWGRZZOYRV`Zc^^aa^[[UceceeccZ_bY]^[\ec`CCCCCCCBCCDDCCCBACC
@HELIUM_000100422_612GNAAXX:7:1:8611:20099#0/1 {"ali_dir":"right","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(C:11)->(A:33)":96,"(T:07)->(C:30)":86},"score":3095,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ccacccatatcttcactccttccctacaacgcgctctgccgatcttatcagcattcggttggggcgacccgccggcggaagctaccttagggataacagatgattaggagatcggaagagcggttcagcaggaatgcggagagcgatacagta
+
############################################?c]URMOIGO_WXY^_cccccb`_dc\adUZX]UJB?>:BOCTUPQa`Y`aDKTXPNIQPKYSV5?CC@@@88>9>>C1<=?###########################
@HELIUM_000100422_612GNAAXX:7:1:8681:5380#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(G:34)->(T:07)":25,"(T:02)->(C:34)":102},"score":5325,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
cccacacgacgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccgtcgtgtggg
+
CCCCCCDEEEc\cbbeJbbaa`a\Gcccc\ccdad^decfdebcdeeecea^edddeeeeeeccLcac_dedde[`cacXVZYLZ]a^^aEEEEEEEEEEELEEEEEFCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8696:8652#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(A:02)->(G:34)":89},"score":5081,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccagcatgcacgggcaatcctgagccaaatcctgctttacgaaaacaagggaaagttcagttaagaaagcgacgagaaaaatggataggtgcagagactcaatgggtgcatgctgg
+
CCCCCCCCa[^XU[bQb_\aeae]_eeeeeac__dbd]_ePX`___ddd^beeccccZ`c_acaccbaa`Y[ZXZa_aaa[]_ccEEELEEEEEEEEEEEEEEEEEDECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:18893#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":73,"pairing_fast_overlap":85,"pairing_fast_score":0.89,"pairing_mismatches":{"(G:31)->(T:02)":37,"(G:34)->(T:02)":34,"(T:02)->(C:34)":107},"score":1290,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
cccatcgctcagatatccgttgccgagagtcgtgtggattaaatagctttgcagcaaggggagcggccagcaagccagtcgtgtccccaagctaggcacagtgttccttgacgccttcggtgagcgatggg
+
CCCCCCCCCCCCCCCCCCCCCCCEDEEEEEEEELEEIEEBEadbd^SZZWMGLIDMLYGQWLNZVBa><A@EEAEECCCCBEEEEEEEDEDEEDEEEEEEEEEEEDLDACCCCCCCCDCCCCCCCCCBCDC
@HELIUM_000100422_612GNAAXX:7:1:8643:7997#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":103,"pairing_fast_score":0.81,"pairing_mismatches":{"(A:02)->(G:34)":95,"(A:02)->(T:34)":98,"(C:02)->(T:34)":105,"(C:34)->(A:02)":28,"(C:34)->(G:02)":12},"score":2764,"score_norm":0.951,"seq_a_single":5,"seq_ab_match":98,"seq_b_single":5}
ccactgacgtcccattgagtctctgcacctatcctttatttattctcgctttatgaaacccttgtttgttttcataaaacgggatttggctcaggattgccccgtcgatctgg
+
CCCCCEEEEEELEEEEEEEEEEEEEEELEEEEEEEEEEabadbbeededeeeddc]dc`bcK^b_beedebba\e_c^BEEEEEEEEEDEEEEELEELEEEEEELEEDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8657:8193#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":102,"pairing_fast_score":0.99,"pairing_mismatches":{"(A:34)->(T:02)":7},"score":3770,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagtcactgcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggcagtgactgg
+
CCCCCCLEEEEEEEEEEEEEEEc`_bc^deeedd_ceecbebbee^eaddedebbececZ_`_X_e]aeZ^^P\]VaEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8700:10551#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":75,"pairing_fast_overlap":90,"pairing_fast_score":0.862,"pairing_mismatches":{"(A:02)->(C:34)":105,"(G:34)->(A:02)":29},"score":3753,"score_norm":0.967,"seq_a_single":18,"seq_ab_match":87,"seq_b_single":18}
ccaccaatcattcggttggggcgaccaaggaaacatcatccttatatataaagaaccattattctcactaaagaccttttactaaaaatcaccaactaagctaccttagggataacaggattggtg
+
CCCCCBCCCCCCCCCCCCEEEEEEEEEELa\]YZVVaUc_caebeeece^edccbWebeeeccee__`[a`ca^_Q[]^^QRRRRccaEEEELEEEEEEEEECELEEECCCCCCCCCCCCCCCC@C
@HELIUM_000100422_612GNAAXX:7:1:8632:5999#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":4880,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cccatctacgcccgaaggcgtcaaggaacactgtgcctagctcgggaatgcggctagtttgctggccgtgccccgtgctgcaatgctatttaatccacacgactctcggcaacggatatcgcgtagatggg
+
CCCCCCCCCCCCCCCCCCCCCCCEEEEcc]cbabaaa]ROLea\bebeace_bWIT]I\]La`ccWabdecdbeebc\]Zbeb_`\^_caZYIIWMYWZYMRRQUeaZCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8628:1289#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4253,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcggccaccgagatccacactctttccccacgcgacgctcttccgatctccgcatgcggatatccgttgccgagagtcgtgttccttggcgtcatcccgcatgcgtggagatcggaagagcggttcagcaggaatgcagagaccgagacggtagccg
+
#################################A)@:>A=B8BCC@CC.eceabdeeeececcefeeeeeeedeeeXeeceececece[ebde`aeecdeb\eaedeb5A6=-==82=6C=C@@#################################
@HELIUM_000100422_612GNAAXX:7:1:8662:15963#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":87,"pairing_fast_score":0.952,"pairing_mismatches":{"(A:34)->(C:02)":22,"(C:02)->(G:34)":108,"(G:34)->(A:02)":24},"score":3256,"score_norm":0.966,"seq_a_single":21,"seq_ab_match":84,"seq_b_single":21}
ccatcgcgagaggatgacgccaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatctctcgcgatg
+
CCCCCCCCCCCCBCCCCCCCCLELEEEEEEDaa`ebVZebbeae\[ZZQ[QYUVUdb^cceX_cc^_aceeacedee^d]cEEEEEEEEEDEEEEEFEEEEEEFEEELCCCCCCCCCCCCCCCCCCC@C
@HELIUM_000100422_612GNAAXX:7:1:8666:2684#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (taccgtg:taccgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taccgtg","obimultiplex_forward_tag":"taccgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taccgtg","obimultiplex_reverse_tag":"taccgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(c:34)->(g:02)":41,"(t:31)->(g:34)":76},"score":1591,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattctacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCACEEEEDLLED?EEEEDDEDD?AEL=BEEA?ECAE?CYQ_R[SSNP^_ccee_cabbcaC`dbNbCCCCCCCCCCCCCACCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:15654#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(G:34)":100},"score":4095,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccacgacgcgagggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatgggcgacacgtgg
+
CCCEEEEEEEEEEEEEEEFEEEec]cad_^dfbbcbcdaZ]_beedcddddedbe`ebcceeeddcceec]_`_ceeeMeEEEEEEEEEEEEEEEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8623:11405#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:34)->(G:10)":46,"(C:23)->(G:02)":19},"score":3510,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccatactcgcggggcaatcctgagccaaatccgtgttttgagaaaacaagaaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgcgagtatgg
+
CCCCCCEEEEEEDEEEEEAEFEEaLaUS]_cbddddNda^XT]P`FaX^X_``]`dd_`dYbadd_``a``c`[cXb``AAA8>A@AAAD?DDDAAAA@BDD?DDBDDBBBBBB
@HELIUM_000100422_612GNAAXX:7:1:8673:5272#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":92,"pairing_fast_score":0.865,"pairing_mismatches":{"(A:02)->(C:34)":108,"(A:02)->(G:26)":90,"(A:34)->(G:02)":22,"(G:35)->(T:02)":23,"(T:35)->(G:02)":20},"score":3493,"score_norm":0.946,"seq_a_single":16,"seq_ab_match":87,"seq_b_single":16}
cctgtaacactgttatccctaaggtagcttatgtttgtagtcttggtagggtctgttgattgattaattgatctttattatgagggatggtttatcctaggtcgccccaaccgaattgttacag
+
CCDDCCCCCCCCCCCCEEEMELMDEEFEEdeceecccc^b_cceea]c]ccaeceebe^ebc_c^a\c_aXY[[]eeeaeEEEE=:=;9DEEEEEEEEEEEEEEEEELCCCCCCCCCCCCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8632:4571#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":89,"pairing_fast_score":0.907,"pairing_mismatches":{"(A:02)->(C:34)":105},"score":3595,"score_norm":0.978,"seq_a_single":19,"seq_ab_match":87,"seq_b_single":19}
ccgcgcattattcggttggggcgaccatggaattcatcatccaccacaaataagattatctaatcactcaaaagatcctaacaatagatcaaagaccaagctaccttagggataacagaatgcgcgg
+
CCCCCCCCCCCCCCCCCCCY\\beKeeaQ`_aRRSQKab]_ceZ]eedeeeedc^_be_b_cca_cc___ddadbCEDBEE?EEEEEEEEEEEEEELEEEEDEELEEDCCCCBBCCCCDCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8675:14585#0/1 {"ali_dir":"left","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":82,"pairing_fast_score":0.899,"pairing_mismatches":{"(C:09)->(A:34)":92,"(T:34)->(A:06)":33},"score":4725,"score_norm":0.976,"seq_a_single":26,"seq_ab_match":80,"seq_b_single":26}
ccagacacgctggatgacgccaaggaacacgataaaagctgaggcaccggcgagccgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatcagcgtgtctg
+
CCCCCCCCCCCCCCCCCCCCCCCCCC`aecccG``aZdbV^^LV[L]XZ`]`bcac^bcdbeOdWebdaec`eedbccaeecaeebbea^RFLPQb`\XXX\dEEEEECCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8660:9491#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4639,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccgattctattagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacctagaatcggagatcggaagagcgtttc
+
AC=ACA@CC>A5CB>6=8edaebdeeeee[cedceeeebeee]ddda]eceeeeeeeeeeedeefdeeedaadeedadc[_cEEEEEEEEEEEEEEEEEEEEEEEEDE##################
@HELIUM_000100422_612GNAAXX:7:1:8630:11024#0/1 {"ali_dir":"left","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":94,"pairing_fast_score":0.901,"pairing_mismatches":{"(C:34)->(G:02)":27,"(G:34)->(T:02)":26,"(G:34)->(T:08)":52},"score":4977,"score_norm":0.968,"seq_a_single":14,"seq_ab_match":91,"seq_b_single":14}
caccgaaggcgtcaaggaacactgtgcctaacctagggacgcggctggcttgctggccgcaccttgggttgcaatgctatataatcaacacgactctcggcaacggatatctgtgcgactgg
+
CACCCCCCCCCCCBEEEEEEEEEEELLEEEEEEeeeed]edec_ca_LQNQFddbd`aebcbeee^eeeedeebadeeeeeecbd__^__bcdbXcecaa`ccc_W\WCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8633:1250#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":100,"pairing_fast_score":0.948,"pairing_mismatches":{"(A:02)->(G:34)":104,"(C:02)->(T:34)":107},"score":4724,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
tgcgatctccacgtcgtacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgagatcgga
+
########EEEEEEEEEEEEc_Zadcca^^`^M\V[a[a]T\Keeececeeeceaabb][]Xc_ac\^`ecebbcca^e_beca_ecbdbeEEDECEEEDEEDLEELE########
@HELIUM_000100422_612GNAAXX:7:1:8627:5905#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":89,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:28)->(T:30)":44,"(G:02)->(A:34)":106},"score":4996,"score_norm":0.978,"seq_a_single":19,"seq_ab_match":87,"seq_b_single":19}
caccacgctcttccgatctccttcggtcggtagtactctggcgtatattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagaccgaaggagatcgaaggagcggtaag
+
########B@BCBA;CACBadcbcecbcebccdd`bcb`bbec?edeeeeebeeeeeefaeddefedeaeeeebddcU`___U\RQQRQdcc^cEEEEEEEEEEELEE###################
@HELIUM_000100422_612GNAAXX:7:1:8652:10403#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(A:02)->(C:33)":97,"(G:31)->(T:02)":37},"score":2653,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
ccagtctctcggatatccgttgccgagagtcgtgtggattatatagcattgcaacccaaggtgcggccagcaagccagccgcgtccccgggttaggcacagtgttccttgacgccttcggcgagagactgg
+
CCCCBCCCCCCCCCCCCCCC@CCEEEEEECEEEDBEICECEEaaa]Z_b^d]ZVWU_[P\`C@W[MV[\YNM>MGFPX[Va``\aeVaecCEEEEEKEDEECCCC>EECCCBCCACBCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8688:10143#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4900,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccgaagtagttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccctacttcggagatcgcaagagcgtttc
+
575=:==?9/<2?:;/>@YYIYX]_bdbddcdfedeae`cZeb_eeebeceefeeeeeedeeadddddeeed`_a_cc]]`caX[[YS]aEEEEEEEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:9085:5501#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(c:30)":58},"score":2,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
98:8;<7<B:BBB<B>B>BK4G@@AA<:D@DDA=<3A808920@@@:8CC%%DDD>AHA>>=?EEEEBEDBECEEEEEECBCBCCCC@C@CBACC@ACCC
@HELIUM_000100422_612GNAAXX:7:1:8695:8859#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":73,"pairing_fast_score":0.829,"pairing_mismatches":{"(A:34)->(G:02)":45,"(C:02)->(A:34)":104,"(C:02)->(G:34)":93},"score":2775,"score_norm":0.959,"seq_a_single":35,"seq_ab_match":70,"seq_b_single":35}
ccatgcagtgcgcacggcatgtgccaaggaaaacaaaacataggaaggtcgcgtctcgtgatgcctcgttcgcggggtgtgcatgggtcgtggcctctcaataaccataaacgactctcggcaacggatatcgcactgcatgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCC<CCCCCCCCBDDDEE=EBLEEE]bec]beeaacb`S`bceee_ac`cc[c`Xda^^X]a_WW\aEELEE?EEEEEEDLEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:12353#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5713,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
tccctccgacgctcttccgatctccgaagtagcaagaagaccctatagagctttatctacccatatgatatcatattcctagattcggttggggcgacctagatctggagatcggaagagcggttcagcag
+
#########?)?<A:A@<7<<<:Raa[^ccaXdd`^`beeeedeeeeeeTd`ecaaa^b\ecdeecfbeeeaceeeeec_ad`ac_\`d^dcdca`^]SX]\[YXXYSAA#####################
@HELIUM_000100422_612GNAAXX:7:1:9392:2194#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ggaggaa:ggaggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggaggaa","obimultiplex_forward_tag":"ggaggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggaggaa","obimultiplex_reverse_tag":"ggaggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"score":1485,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEDDEDD@EDF?EDDE9EEEEDD=YXVa_`_`_caLcaebXe`a]QaDCEECCCCCCCCCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8963:10044#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgcca:ttcgcca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgcca","obimultiplex_forward_tag":"ttcgcca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgcca","obimultiplex_reverse_tag":"ttcgcca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(G:32)":56},"score":1315,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCDCCCBCEEEEBEEEEcc_de`\[d_\ecZebddaEBEBBEEDJCEEEEEEEEEEEEEEEEEEELEEAECCCCCCBCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8646:19217#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":4046,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccacgtgtacggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatgcaaaggaaaaggataggtgcagagactcaatgggtagcgagtgg
+
CCCCCCc]_ccbbceeeeccaNZMeebecZbceceeeefeedcebdedddeeeeceeebbeebDEEEEEEEEEEEEEEEEEEDEEEEEEEEEEEEEEEEEEEEEEEEECCCDCB
@HELIUM_000100422_612GNAAXX:7:1:9358:13395#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3806,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
atacagcgaccacccagatctacactctttccctacacgaccgctcttccgatctccacggtacttagataccccactatgctagaggagcctgttctagtaccgttgagatcggaagagcggttcagcaggaatgccgagaccgatatagtatgctgtcttc
+
###################B9;>=A=A901/00(+/109@C*CCCCCCCC@CCBCeeceeeeeeeedcce_eeaeeeceeedeeeeeeed_deeefecee`eeee]H^>?86*77BBBB@==9A>,0000;::3:99/6;=A=2A##################
@HELIUM_000100422_612GNAAXX:7:1:9068:14378#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aatgtcc:aatgtcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatgtcc","obimultiplex_forward_tag":"aatgtcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aatgtcc","obimultiplex_reverse_tag":"aatgtcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:07)":49,"(T:02)->(G:10)":51,"(T:25)->(A:02)":38},"score":358,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtgcgaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
C6CCBCCCCCC?CCBCCCCEAEEEEEEE@?@;@291AC99:VKb^__,12411A:(8;BD@DBD>BBBCDCDBBI5@>@DDBBBB?B=BBBB>>:>>///
@HELIUM_000100422_612GNAAXX:7:1:8719:3212#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgatga:gcgatga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgatga","obimultiplex_forward_tag":"gcgatga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgatga","obimultiplex_reverse_tag":"gcgatga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{"(a:02)->(c:34)":80},"score":3489,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCACCCCCCCCCCDCCCCEEEEa^SY[_^_b[SYFIXdXcbfeebecccd`aZd]___eebUd]bdZcbcae`\aQaELE?CCCCCCCCCACCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8678:1288#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4319,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gccaccacccagatctacactctttccctacacggcgctcttccgatctccggttcacccgtatatacatacgtatacgtctgaatgaaagagttggtggccgcgtggagatcggaagagcggttcagcaggaatgccgagacagatatcgtatgcg
+
##############?);?7A:A<A2<AC==C@C:/=CC@@BACCC@CCCbeeeeeeceeeeefeeebeeccedeeeeee_eeccacdcceeeedceebeedceeeefdCA=;9>=B;AAA>AAA=6=9800)0,B=?####################
@HELIUM_000100422_612GNAAXX:7:1:8627:4404#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":4423,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
ccacgctcatgccattgagtctctgcacctatccttttttgattttaactttctgaacttttatttgttttcagaaagaaggatttggctcaggattgccccatgagcgtgg
+
CCCCEEEEEEEEEEEcZdbeeecZZ_c\ccc_]edeeddcb\addc]_bdeeeec]b_bbeedcc_dced]^]bcc`cEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8637:5483#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(G:34)":100},"score":2504,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccacgctctcagggcaatcctgagccaaatccttctttctgaaaacaaataaaagttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggtgagagcgtgga
+
CCCCEEEEEEEEBEEEEEEEEEEEEEEedebeabbacb_ZUPZab\QVdabc\Y]\T_\_dd]ccBBBBBCEEEEEEBEEDDDEEDEEEBEEEAEBEECLEEDBDAA?A?AA
@HELIUM_000100422_612GNAAXX:7:1:8667:4186#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5200,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccagatgcgacgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatgggtcgcatctggag
+
##EEEEEEEEededeceeee[eaeee_eeeecbQQQPQ^d]]_ddeedcddefeddeceececcc[]^`aaaeeeadYYZY[QORRQbdEEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8998:15541#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtaggt:tgtaggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtaggt","obimultiplex_forward_tag":"tgtaggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaggt","obimultiplex_reverse_tag":"tgtaggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":42,"pairing_fast_overlap":63,"pairing_fast_score":0.7,"pairing_mismatches":{"(a:02)->(c:34)":71,"(c:26)->(a:34)":54,"(g:02)->(t:34)":80,"(t:34)->(g:02)":31},"score":856,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ctagccgtaaaccttgatagaaatatacaattgatatccgccagggaactacaagcgccagcttaaaacccaaaggacttggcggtgcctcagacccac
+
B@BCBCCCC@CCCCCCCCEDDBE?DJB>EELB5/2111E[FA]ZL?SPWSY^cC]a]aAC?==AA=ECE=L?CC<CBCDLECCCCCCAACCAA;AAC@C
@HELIUM_000100422_612GNAAXX:7:1:9010:19915#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttatgtg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatgtg","obimultiplex_forward_tag":"ttatgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(t:30)":48,"(g:02)->(t:34)":81},"score":1393,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CDDCCCCBCCCCCCCCC?CcDZ]`[W]V_b_bQIHAQRHR[TXU\X[H=?A:BEEEEBEEDDEEDFEEBDEEEEEEEDEALBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:15342#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":85,"pairing_fast_score":0.768,"pairing_mismatches":{"(A:02)->(C:34)":97,"(A:28)->(G:02)":59,"(G:34)->(A:02)":37,"(G:34)->(T:02)":29,"(T:02)->(C:34)":107,"(T:34)->(G:02)":30},"score":1794,"score_norm":0.929,"seq_a_single":23,"seq_ab_match":79,"seq_b_single":23}
ccagtgcatcggatatccgttgccgagagtcgtgtggattatatagcattgcaacccaaggtgcggccagcaagccagccgcgtcctcgggttaggcacagtgttccttgacgccttcggcgatgcactgg
+
CCCCCCCCCCCCCCCCCCCCBCCEEEEELLEDEE?ELBEBEECEDECCEC8;96:E@EFEEAEa_MX\Y[]\LZ[MU[YJZ\\TZeUceeOba_bELEECEEEEEELECCBCCACCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:11590#0/1 {"ali_dir":"left","ali_length":97,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":97,"pairing_fast_score":0.723,"pairing_mismatches":{"(A:02)->(G:34)":92,"(A:24)->(G:09)":43,"(C:02)->(G:34)":108,"(C:34)->(A:02)":25,"(G:34)->(C:02)":12},"score":1756,"score_norm":0.918,"seq_a_single":11,"seq_ab_match":89,"seq_b_single":11}
ccatgcagtgcgggcaatcctgagccaaatccgtgttttgagaaaacaaaggggttctcgaatcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggcactgcatgg
+
CCCCCC??C@CLEDEEEDDEEEEELEEEEAEABa]WR[NLRL<GMPaaRRIOIULZbaW_LPBNOe`b^dCD5DCAECEELEEEEELEEEELEEEELEEEEEEDEEELCACCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:17931#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:34)->(G:11)":15},"score":5217,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagacgagagccattgagtctctgcacctatcctttatttattctcgctttgtgaaatccttgtttgttttcataaaacgggatttggctcaggattgcccctctcgtctgg
+
CCCCCEEEEEEEEcEbZaacddb_dac\bcdcSdbeeeeeddedcddZbeeeeeede_e\eecd^deededadUadcccb\`WRacacKdEEEEEEEEEEEEEEEBEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8614:12499#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":4911,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
cccgacgatctgcaatcctgagccaactccttttttcaaaagaaaaaaaaaagaagggttcagaaagcaagaaaaaataaaggataggtgcagagactcaatggagatcgtcggg
+
CCCCCCCEEEEEeae]WaY]bcYaZ^``U]eeeebc``dddJdccccccddbbd`de[bf^cc_ced]bKbbcbbbbdd`[cPcdEEEEEEEEEEEEEEEEEEEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8623:8362#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":107,"pairing_fast_score":0.923,"pairing_mismatches":{"(C:02)->(A:34)":95,"(G:32)->(T:13)":34},"score":4114,"score_norm":0.981,"seq_a_single":1,"seq_ab_match":105,"seq_b_single":1}
ccagcgatgctgggcaatcctgagccaaatccggttttctgccaataacaaaagccagaaagcgaaaatcaaaaaggataggtgcagagactcaatggagcatcgctgg
+
CEEEEEEEEEEEEEEEEEBda_caLcceecbb_CYeeee`]`eeeddeeeeebb`abeece`baaeeceb_ccc`eXeeEEEEEEEEEEEEEEELEEEEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8685:11783#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4254,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctaccctccttccctacacgacgctcttccgatctccttcacttcaccaactctttcattcagacgtatacgtatgtatatacgggtagatcggagatcggaagagcggttcagcaggaatgccgagacagatatcgtatgcg
+
#######################??;A=:8<8<,)/+/<<<<3@=8CCCbe`ecbc`ccbccceeZeddUeedeeedceefeffdefeeeceeededdeeccebb]ddACA?AA@C@CC=AAAA977::00000>=A8A##################
@HELIUM_000100422_612GNAAXX:7:1:9235:3942#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ggaggaa:ggaggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggaggaa","obimultiplex_forward_tag":"ggaggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggaggaa","obimultiplex_reverse_tag":"ggaggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"score":1072,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCDCCCCDCCCBCT^`eZcccY]d[e_bV?AA?CE=ACCD?A@9A9AD?DEEEE@EEEEDEEEEEEEEEEECEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9017:10856#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacattg:aacattg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacattg","obimultiplex_forward_tag":"aacattg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacattg","obimultiplex_reverse_tag":"aacattg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(A:02)->(G:07)":52,"(G:02)->(C:10)":55,"(T:33)->(A:02)":21},"score":593,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactgcccgcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCCCCCCCC@C@DK@D4EEECcR]caURRb]<CA:?CA?A221/1<74A<;<AA5BEEEEECE?CDDCDA=EELCCC<BCC;CCCCC@BCCCC
@HELIUM_000100422_612GNAAXX:7:1:9021:16140#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aaccgcc:aaccgcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccgcc","obimultiplex_forward_tag":"aaccgcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccgcc","obimultiplex_reverse_tag":"aaccgcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":1806,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCC@CCCCCCCCCCCCCCCeV_cS_ZbJ\\XPXSBSRWMQ\US\\YZUUVRR5:9<--C??697>><DEE>EBEEDB<EELCC@CCCC@6CCC=BCCCCB
@HELIUM_000100422_612GNAAXX:7:15:15309:5584#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":3183,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgcacaccgagatctacactctttccctacaccacgctcttccgatctccgcctcctttagataccccactatgcttttctagaggagccctgttctaaggaggcggcgatcggaagagcggttcagcaggaatgccgcgacagatatcgtgcgcc
+
###################@=C@BCCCC;AA9A6AAAA@;2CA@A<CCAdd\Yd^bdddad^WdYc``cddcd\cdRQ`Y][aadcT`ad`bbb^MUaEEEEEEEEEE#################################################
@HELIUM_000100422_612GNAAXX:7:1:8634:7952#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":102,"pairing_fast_score":0.889,"pairing_mismatches":{"(C:02)->(G:34)":90,"(G:02)->(T:34)":99,"(T:02)->(G:34)":106},"score":3359,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
cccacacgcgaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccctcgcgtgtggg
+
CCCCCCEEEEEEEBEEEEEEEEEEEEEEEE_ZT_`Zaccebdee_bWe`daeabcccXe[ede_cb]ebdeb^eebabcEEEEEEEEEELEEEEEEEELEEEEEELEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8681:12056#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(C:02)->(A:34)":94},"score":4587,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tccgatcgtgcaggggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggctgcatcgtggagatcgga
+
########EEEEEEEEEEcc]b]\^VP]QPNRR]cc_cebabbddeedcbcdeebdbdeeeebbeb^ebddcded]be\[US[YXY[ZaEEEELEEEEEEEEEEEEEE########
@HELIUM_000100422_612GNAAXX:7:1:8642:5051#0/1 {"ali_dir":"right","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":92,"pairing_fast_score":0.989,"pairing_mismatches":{"(T:02)->(G:34)":108},"score":5197,"score_norm":0.989,"seq_a_single":16,"seq_ab_match":91,"seq_b_single":16}
acgacgtctgcgatctccatgcagacaagaagaccctatagagctttattcactaagctatataaactcttactcttaatgaattcggttggggcgacctctgcatggagatcggaggagcgct
+
################EEa^Zc^ebeaeeaaeeeeededdddbeeeeecce]Zc`bW_bcdeefedededaeee`f]a^U^VOZZ]RRQRR__`[aEEEEEDEEFEEL################
@HELIUM_000100422_612GNAAXX:7:1:8693:16636#0/1 {"ali_dir":"right","ali_length":77,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":77,"pairing_fast_score":1,"score":5372,"score_norm":1,"seq_a_single":31,"seq_ab_match":77,"seq_b_single":31}
actctttccccacaccgcgctcttccgatctccagagtagcgccattgagtctctgcacctatcccaaatctggcacgacctggctcaggattgcccgcgtactctggagatcggaagagcggttcagaaggaatgcgg
+
##################AA<B<<><72<A@be\ea_caaaXKdddc`ebeeeebadededeeeeeeeecbeeeeeeeegceeeee[__Y`c\ccbadac]a\\`_ebC?C3795;5A?####################
@HELIUM_000100422_612GNAAXX:7:1:8620:9963#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":5011,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccgtgcctcggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaagaggcacggagatcgcaagagcgtttc
+
################CAa\_\_eeeeceebbc^^`_^a]_`cedebdeecedddeedeedeedceeeeeeee_cccNaa]cURRQQRccccEEDFEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8705:13648#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":89,"pairing_fast_score":0.756,"pairing_mismatches":{"(A:02)->(C:34)":106,"(A:02)->(T:34)":108,"(C:34)->(G:02)":32,"(G:34)->(T:02)":31,"(T:34)->(A:02)":25},"score":1236,"score_norm":0.91,"seq_a_single":19,"seq_ab_match":81,"seq_b_single":19}
ccaacaggttacaaccaaatccaatttcatgccgatattaaaatcaacaatccaaataaacgaataccaatgtaatccatcattccacttagaaacaagctgcaccttgacctgaaatacctgttgg
+
CCCCCCCCCCCCDBCCCCCLEEEELEEFEELLEDEEcQaX]aZLRMZ_\]TPRQa[b\`[=.@CCCCLDECDBCEEELDDEEEEEEEEDEEEEEEEEEEEEEECELELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8698:3660#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":3886,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatcttccctctttccctacccggcgctcttccgatctccttccgctcaccaactctttcattcagacgtatacgtatgtatatacgggacgactggagatcggaagagcggttcagcaggaatgcggagaccgatctcgtatgcc
+
#################################################EaWaac_`\QLRRLbeeebaeeec`cb`]eecee`eeaeddd`dab[eedebeeZeccc?CC=C?3?8?<CCCCCC=?@C;>;?::8<);>A>A'CC@##########
@HELIUM_000100422_612GNAAXX:7:1:9345:13603#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:02)->(a:34)":74},"score":2323,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCACEJEEEEEEEccc`RPNKRJYY]UNPOPJORVWSabcbc]aaRY]X]`\^dcdEELEEEEEBECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:1989#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4341,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgcccacccagatctacactctttccctacacgacgctcttccgatctcccactagcgtgatatccgttgccgagagtcccactgtgttccttgacgccttcggacgagatcggaagagcggttcagcaggaatgccgagaccgatatcttatgcg
+
#################A8ABC?3897662A==<=C@@CCCCBBC;CCCeeedbbeeeeeaeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeeddeeeeeeeeeab@BA3:A?C=CACCCCC#################################
@HELIUM_000100422_612GNAAXX:7:1:8632:17367#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":73,"pairing_fast_score":0.786,"pairing_mismatches":{"(A:02)->(C:34)":96,"(C:02)->(T:34)":99,"(G:02)->(C:31)":91,"(G:34)->(T:10)":45},"score":2741,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacgactagcgatatccgttgccgagagtcgtttatggttaatgagaggccacgacccgtgtgcacaccgaaaacggggcaacacgagacgcacccttcttgagttttgttttccttggcacatgccgtgcgctagtcgtgg
+
CDCCCBCCDBCCBCBCBCDCCCCCCBCCCC@CCCCZa_XXLV[_Fac`_TNQ]eReeceZee]_ab___`]ceece_aeeEEEEEC?EEEIEEEDLEELEEEFEDDDDCCCCDCCCCBCCCCDCBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8652:17625#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":100,"pairing_fast_score":0.99,"pairing_mismatches":{"(C:02)->(G:34)":108},"score":4152,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tgcgatctcccacgtagctgggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatggagctacgtgggagatcgca
+
#####??7SI[TY`VcQZ^eZca\]_S]aY`W[e\eVbe`beacc\c^[eaeecee]c`bc`bU^S[\eeeaeDEEEEEEEEEDEEEDEEEEEDEEDEDEEEEEEEEL########
@HELIUM_000100422_612GNAAXX:7:1:9062:15398#0/1_sub[83..89] {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:cgatctc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"cgatctc","obimultiplex_reverse_tag":"cgatctc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3898,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
cctctaa
+
eeeecee
@HELIUM_000100422_612GNAAXX:7:1:8785:19748#0/1 {"ali_dir":"right","ali_length":10,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":2,"pairing_fast_overlap":10,"pairing_fast_score":0.286,"pairing_mismatches":{"(A:34)->(-:00)":102,"(G:34)->(A:34)":104},"score":314,"score_norm":0.8,"seq_a_single":98,"seq_ab_match":8,"seq_b_single":99}
cggaaaaattgccgatttctacgaagatgaggtcgatcaggcggtggcctcactgacgtcattactcgaacccatgatgatggtgttccttggcgtcatccacrcatgcggatatccgttgccgagagtcaggtgttccccaagatggtcacgcacatgattgccgtgggagaatcaacgggcgcactcgatgcaatgctcgaaaca
+
################################################CCC>C7@=CC5CC@C;@@AA+8@@CCCCCCC?CCCCCCBCC@C@CCC?CCCeeCeCeeeeeCCCCCCCCCCCCCCCCCCCCCCCA@CCCCCBCC?CC6CAC9>7;9AA###################################################
@HELIUM_000100422_612GNAAXX:7:1:8682:18378#0/1 {"ali_dir":"right","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":87,"pairing_fast_score":1,"score":5753,"score_norm":1,"seq_a_single":21,"seq_ab_match":87,"seq_b_single":21}
cacgcgacgctcttgcgatcttcagatcgcgaccattgagtctctgcacctatccggctactcgtgtcccttcgccggacttggctcaggattgccctcgcgatctggagatcggaagagcggtgcagc
+
#########?9?08+78);C9bbccc_c]]LSScbK]cX[ece]``aXaecebeebeeecebaebe^eededbed`edc]eccaebcc_Zc][Z_\db^eeUXUR^c^;A###################
@HELIUM_000100422_612GNAAXX:7:1:8702:19544#0/1 {"ali_dir":"right","ali_length":75,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":75,"pairing_fast_score":1,"score":3675,"score_norm":1,"seq_a_single":33,"seq_ab_match":75,"seq_b_single":33}
acactctttcccgacacgacgctcttccgatcctcgccccaaccgaatccatgataagttagttatttatttagggttaagctctatagggtcttcttgacttagtggagatcggaagagcggttcagcagcaatgcggag
+
#################################DDDDD`dabdab_\^cdcdbX\\[][X^]]daa`d`ddZYd^Wd^baaWbbbddYdab__EDEDDEEEEEFEEED#################################
@HELIUM_000100422_612GNAAXX:7:1:9255:9942#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:ttctaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttctaac","obimultiplex_reverse_tag":"ttctaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4190,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ccttcta
+
]aaceec
@HELIUM_000100422_612GNAAXX:7:1:8639:12548#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":87,"pairing_fast_score":0.905,"pairing_mismatches":{"(A:02)->(C:34)":100,"(T:32)->(G:02)":32},"score":3476,"score_norm":0.977,"seq_a_single":21,"seq_ab_match":85,"seq_b_single":21}
ccgaggcttctgttatccctaaggtagcttgtttttttgatctgggtcagggtcttttttgagatgtatatttctttttgtgggtgatgatttgtagagcatcggtcgccccaaccgaataagcctcgg
+
CCCCCCCCCCCCCCCCCCCCCEEEEEEEEEEJEEbbb[]daea_aaX][___^d_eeeaXX`_YYd\cdaddbddedddd`ca_aEEEEFEEEEEEEEELAEDEEEEECCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8653:5548#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4783,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcctccgatctccgaatggtggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaccattcggagatcggaggagcggttc
+
##############@B>>XZYVY]KWa`Ud^d`S[ZXYXa^`[bbc]]eeaec_^eebbeeeddacd_ceaaeaaaTc_]Xca]^^Y[c]`cDEEEEEEEA>EEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8691:20860#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":108,"pairing_fast_score":0.581,"pairing_mismatches":{"(A:02)->(C:33)":96,"(A:22)->(G:02)":43,"(A:32)->(G:02)":11,"(C:02)->(T:34)":95,"(C:09)->(G:30)":52,"(C:32)->(G:02)":8,"(G:20)->(T:02)":12,"(G:25)->(T:02)":34,"(G:31)->(C:02)":17,"(G:31)->(T:02)":14,"(T:02)->(C:34)":85,"(T:19)->(G:02)":15,"(T:31)->(G:02)":24,"(T:32)->(A:02)":19,"(T:32)->(G:02)":1},"score":1218,"score_norm":0.852,"seq_a_single":0,"seq_ab_match":92,"seq_b_single":0}
tccgttgccgagagtcgttacagtaagtacgacgggagccgcacgcccgcggagcgcgggaacgcggcgtcccactttgtgttccttggcgtcatcctgctgtcgtgg
+
JCCCCCCJ?<J>@I=BIBJ<CCCICCCCCC7=@C:-;;<853@^^Z`aTb\BXRW^^c_`]]c`c`EEEECCEDAECEBDEEEELEEEEEELEELKEDEEEEEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:8612:6691#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":85,"pairing_fast_score":0.951,"pairing_mismatches":{"(G:18)->(C:02)":33},"score":3067,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
cctacacgacgctcttccgatctcctctgagggaagaagaccctatagagctttatctaaagtttaatatatctaacccaagattcggttggggcgacccctcagaggagatcgcaggagcgcttcagcag
+
#######################EEDEEEEEE<C`P`c`Y`[^cccaa^^^[]acc_d]_S[V][W\\dad[aP\[ZWW^`aDDCDCDA;DAA>>@3:CCBACCDCDD#######################
@HELIUM_000100422_612GNAAXX:7:1:8672:4629#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":3836,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccactctgcacgggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatgggcactcagtgg
+
CCCEcaZ_ceeWZYXU\_W_c\\XW^LRJQQ^\^UPccbabYdee_adbdbcZcceebeeeeb`fDDDDDEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8644:7861#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":73,"pairing_fast_score":0.943,"pairing_mismatches":{"(G:34)->(T:02)":45},"score":2185,"score_norm":0.986,"seq_a_single":35,"seq_ab_match":72,"seq_b_single":35}
ccacactgtcggatatccgttgccgagagtcgtttatggttattgagaggccacgacccatgcacaccccgcgaacgaggcatcacgagacacgaccttcctatgttttgttttccttggcacatgccgtgccgacagtgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCC;CCCCCCCCC?ECEEEEEEELEEEEFEEEEEBEBb_Zbadd^eecbS_^a_]cb`a^[Z]^cRcWb`ADC;EEEEEEEEEDDDDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8702:19656#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":93,"pairing_fast_score":1,"score":5464,"score_norm":1,"seq_a_single":15,"seq_ab_match":93,"seq_b_single":15}
acgctcttccgatctccagctgacgcccattgagtctctgcacctatcccccttttcactttgttttcggaaaataggatttggctcaggattgccccgactacgtggagatcgaaagagcgg
+
#############@?Se\ca]Zbeedee^aaUX_dddc`Xa]a^a]`Zbceeedeecccddeededddadeebee]ZX[[`aa`Z^^]][^\Z^\XaEEEEEEEEEEE###############
@HELIUM_000100422_612GNAAXX:7:1:8695:9965#0/1 {"ali_dir":"left","ali_length":65,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":43,"pairing_fast_overlap":65,"pairing_fast_score":0.694,"pairing_mismatches":{"(A:06)->(C:32)":73,"(A:25)->(T:11)":56,"(C:02)->(G:28)":92,"(C:02)->(G:32)":102,"(G:02)->(A:33)":106,"(G:02)->(T:32)":103},"score":1470,"score_norm":0.908,"seq_a_single":43,"seq_ab_match":59,"seq_b_single":43}
cccagtctcgtgatatccgttgccgagagtcgttttgacatatagaatacagaatacgacgcgcaccccgaccgcgcaacgtatctcggaggcgggggggggtgaatgccaattcgttaggtgttccttgacgccttcggacgagactggg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC@CCCCACC@8CCCc_ceeabO6<=@<LDKJVOH7LPYVU\:EE?P[YZZ3DBDEEBEDE?DFB4BB:DEECJJADKBECC@CCCCCCCCCCC@CCCCBCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9172:14735#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgagt:ttcgagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgagt","obimultiplex_forward_tag":"ttcgagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgagt","obimultiplex_reverse_tag":"ttcgagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(C:02)->(G:34)":56},"score":1490,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
BCCCCCCBCCCCCCDCCCCebcdbcddbeddda]bWdaV_U]EEEEEEEEEFEEDLBDLEEEEEEEELEEEEEELELLEELCCCCCCCBCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8610:4347#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4920,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
tctcccagactgtcccattgagtctctgcacctatccccttcctatgctaggagaagaaaccattgtctccacgaaccggatttggctcaggattgcccgacagtctgaga
+
7A<W^]^Rb]_eecdcdbbJeeeeeebeeb_ebcaceed_eeeefe`ddfdeeeeeadaeb_ebcbbbccbecEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE###
@HELIUM_000100422_612GNAAXX:7:1:8650:16135#0/1 {"ali_dir":"right","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":82,"pairing_fast_score":0.848,"pairing_mismatches":{"(A:34)->(C:09)":53,"(G:34)->(A:06)":37,"(G:34)->(T:22)":30},"score":4349,"score_norm":0.963,"seq_a_single":26,"seq_ab_match":79,"seq_b_single":26}
ttccccacacgacgctcttccgatctccagagtagcgccattgagtctctgcacctatcccgccggacttccaacgggacttggctcaggattgccccgctactctggagatcggaggagcggttcagcagcag
+
###AA->C?60?@+>>>>>AA0?=A@_c[C_`Z]UXGWaP\cWY\]Y\ZZV\F\^XRNcee_ee`fcabeebeeceebecc`bdaaaa_R`__][`dbdEEEEEEEEE##########################
@HELIUM_000100422_612GNAAXX:7:1:8632:8871#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":96},"score":5143,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagtcgtcacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgtgacgactgg
+
CCCCCCEEEEEEEEEe`^eceeee_`Q_a_c\M_`ece^eceee_a_cT_cc`deeeeeaece[cdceeeebdee_cccceeabcc_bcEEEEEELEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8880:4054#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gatgacg:gatgacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatgacg","obimultiplex_forward_tag":"gatgacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatgacg","obimultiplex_reverse_tag":"gatgacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4069,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CBCCCCCCCCCCCCCCCCCEEEdddbdbcdddbbP]cc`_c`^Zd``[aec_febadbee_Zbc^acdbeededZb\dcc[CCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9147:11482#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gccgtgc:gccgtgc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccgtgc","obimultiplex_forward_tag":"gccgtgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccgtgc","obimultiplex_reverse_tag":"gccgtgc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(a:27)->(c:32)":72},"score":1341,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCELEEEEELEEEEEDDDFEDAEBECAEEC?EEEEEaOZWKEMLJI[aZa]_]WAdeXcM_`ZEC85894??AAAAC87CC4C
@HELIUM_000100422_612GNAAXX:7:1:8699:3638#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4341,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctacactccttccctacacgacgctcttccgatctccgatgacgcaccaactctttcattcagacgtatacgtatgtatatacgggtaccgtggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgcg
+
#############?:;;?//00*?<)<?9;?B?+@CCCAB@B6DBABCCeedcbedeeeddeaeeeeefeecdeeeeeedfdeedeeeeeeeeedfddeeceeeceeeCA><3=9A=A?A;AAA<37::0000+:7:39?#################
@HELIUM_000100422_612GNAAXX:7:1:8648:8406#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":102,"pairing_fast_score":0.879,"pairing_mismatches":{"(G:34)->(A:02)":12,"(G:34)->(T:12)":35,"(T:02)->(G:33)":103},"score":3536,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagacgacgagggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtcgtcgtctgg
+
CCCCCCEEEEELEEEEEEFEEEEEEaaa^RRRMMEcca_aa[]aaYaZ]cd_^[_aea\[`d^[a``ca^\[_R`[ccc_aJ\>>CCD>CACCCACDDDADDKDADCBBBB@BB
@HELIUM_000100422_612GNAAXX:7:1:9221:10579#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(c:02)->(t:34)":81,"(t:32)->(a:02)":38},"score":1492,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCCCCCCCCCCCCCEAEECCECEEEEBB@@CEJ1<A9+CC@A;?AD@d\aQ_SX]SVYc\\`a^cSW`bcbEEEALCCCCCCBBCCDCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9373:10234#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtgagcc:gtgagcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtgagcc","obimultiplex_forward_tag":"gtgagcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgagcc","obimultiplex_reverse_tag":"gtgagcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(C:25)":64,"(A:02)->(G:17)":52,"(T:34)->(A:02)":21},"score":604,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattaaataaacaaaattattcgccagagtactgccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
BCCCCCCCCCCCCCBCBCCEL:EAEEEC@?C?D9;?A\R_Y^a\\caA8>>;@A@+??AB@<@C<?A??>EADBDDG?EELBCBCCCBCCCCCC@ACBCC
@HELIUM_000100422_612GNAAXX:7:1:8775:1302#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:aacgagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgagc","obimultiplex_reverse_tag":"aacgagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:34)->(G:02)":29,"(T:26)->(G:02)":35},"score":548,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCBCCBCCCCCCCAC?BEEE@EEEECLEC9EAD>DC8C?@ZZbYbRVZV<CEEEC7E8C=EEEECE?EEEEE@EJCE>ECDCCCC;CBCCCC@;CCCC
@HELIUM_000100422_612GNAAXX:7:1:8664:12964#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":73,"pairing_fast_score":0.829,"pairing_mismatches":{"(A:10)->(C:27)":70,"(C:02)->(T:34)":95,"(G:34)->(A:19)":46},"score":2276,"score_norm":0.959,"seq_a_single":35,"seq_ab_match":70,"seq_b_single":35}
ccacgcatcgagatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcgtttattgttttccttggcacatgccgtgctcgatgcgtgg
+
CCCCCCCCCCBCCCCCCCCCCCCCCCCCCAC?CCCZ_`^VXZ_]_D_dV\X_^XKSZNT^^YRVVPZKE?X_YaVX[QaECEEEEEEEEEEEEELEEEEEEEEEEEDDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC@CC
@HELIUM_000100422_612GNAAXX:7:1:8688:6952#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":4279,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccagctagtgcccattgagtctctgcacctatcctttccctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccgcactagctgg
+
CCCCCCEEEEEEE:EEEebccc`]ffbeddeeeeedefeebeeedeeeecbcdfeebedeeeeeddfedeeedecDEEEEEEEEEEFEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8684:19057#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(T:02)->(G:34)":87,"(T:34)->(G:11)":36},"score":5054,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
cccagtactcgccattgagtctctgcacctatccttttttattataactttctgaatccttggttgttttcagaaaacaggatttggctcaggattgcccctgctgatggg
+
CCCUceedeee\^dbeXa^aYa__U__c]ccaeb]E`eeceaadeeWddededa_dec_eeeaa]ebee`YZ_aceeeEEEEBEEELEEEEEEDEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8701:13156#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":104,"pairing_fast_score":0.97,"pairing_mismatches":{"(A:34)->(G:02)":7},"score":5417,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
atctccacgagcgtcgggcaatcctgagccaaatcctgaaacaaaaaaaagttcagaaagtgaaaataaaaaaaggataggtgcagagactcaatggactcgctctggagat
+
####EELEEEEb\[Q\[`^_\\ZdaeeeWecceedeeeadbdaddddddde`e__aabaeaaaacccbaeddddd]cdd[\Z][Z[^][_aEEEEEEEEEEEEEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8631:17495#0/1 {"ali_dir":"left","ali_length":72,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":72,"pairing_fast_score":0.986,"pairing_mismatches":{"(A:33)->(T:22)":37},"score":3258,"score_norm":0.986,"seq_a_single":36,"seq_ab_match":71,"seq_b_single":36}
ccactgcgtagccattgagtctctgcacctatctttaatattagataaaaaaaaatattatttcttatctgaaataagaaatattttatatatttctttttctcaataaagaagatttggctcaggattgcccgcgtcagctgg
+
CCCCCCCCCCCCCBCCCCCCCCCCCCCCCCCCCCCCBS]]Z[VR^ddcdYX_\]^YbI`eeebed^a\]^QV[ZZ\\U^]cbc_ccccXcEDD@DDDEFEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9235:8379#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(a:02)->(c:29)":63,"(c:07)->(t:02)":48,"(t:30)->(g:02)":38},"score":183,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagcactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
A;ACCCCCCCCCCCC@C=CEL>LEEEE@EEDCEC@?EH7<A03@?=@1?A2@@?_VIX??<?G:A@CC=ECEEEEBEEB?E@C?CCBCAC@CCCB>AACC
@HELIUM_000100422_612GNAAXX:7:1:8632:7806#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":4707,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctaccctactctcttccgatctccggtctggggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgccagaccggagatggaggagcgcttcagcaga
+
#############D@@?CCACCCcccccb]eebfbceeeeece_deeeee_efdegeeeeeeeeeaeeeeeeeeededebdUe`^ccaEEEEDEFDEEEFFDEEADDE#######################
@HELIUM_000100422_612GNAAXX:7:1:8897:12369#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:33)->(C:02)":27,"(A:34)->(T:02)":19},"score":546,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CACCCCCBCCCCCCABACLEEEEBEEK=EEEB^MX[[Zc^_EEECEA/;=2<C=??BEBEEEEEEE8<9>;EEEALDEEEECBCCCCCCCCCCC=CBCC
@HELIUM_000100422_612GNAAXX:7:1:8687:14445#0/1 {"ali_dir":"right","ali_length":68,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":68,"pairing_fast_score":0.938,"pairing_mismatches":{"(C:07)->(T:34)":105},"score":4565,"score_norm":0.985,"seq_a_single":40,"seq_ab_match":67,"seq_b_single":40}
cagatctgcactccttccctacacgacgctcttccgatctcccacgtagctgatatccgttgccgagagtcagtggggtgttccttgacgccttcggagctacgtgggagatcggaagagcggttcagcaggaatgccgagacggata
+
###############ABAC2BC@C8CCC?2;/=A;*8?A:cca]edeeecdeeee[deeebedeeeecede^__eeeeW^edeeeeaebacWd`b^[[KU_ca]GdaU?A:;AA##################################
@HELIUM_000100422_612GNAAXX:7:1:8690:4758#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":4112,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
ttcgatctccgacgcatcgggcaatcctgagccaaatcctgttttacgagcaattcaaaaaggaatcaaaaataggataggtgcagagactcaatgggatgcgtctggagatcgga
+
########EEEEEEEEEEccZY_[]V[[cc[MdVcbeeedeeedddb`e`deeedbeeeeeecedd]ceeeee]]KcdEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE########
@HELIUM_000100422_612GNAAXX:7:1:8685:19992#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":104,"pairing_fast_score":0.99,"pairing_mismatches":{"(G:02)->(C:34)":108},"score":5526,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccacgtcgagtgggcaatcctgagccaaatcacgttttccgaaaacaagcaaaggttcagaaagcgaaaagaaaaaaaagataggtgcagagactcaatggactcgacgtgg
+
CCCCEEEAaYcZL]bP]]\__ScZdeecbeeacZU_Qeaab_ecebdHbc__cca]c]`cceedcdcbdPdd^^_bbb^bYU\`Q\aa_a`ccEEEEEEEEEEEEEELCCCC
@HELIUM_000100422_612GNAAXX:7:1:8628:11553#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":98,"pairing_fast_score":0.832,"pairing_mismatches":{"(A:02)->(G:34)":86,"(C:02)->(A:34)":104,"(C:34)->(A:02)":25},"score":3157,"score_norm":0.959,"seq_a_single":10,"seq_ab_match":94,"seq_b_single":10}
ccacagcgacagggcaatcctgagccaaatccgtgttttgagaaaacaaagaaaggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtgtcgctgtgg
+
CCCCCCCCCCEEEEEEEECEEEEELEEEd]^_b`^^ab`PedWdaed`dbdba\aaceaeec`U`b_Y^`ebccbEEEELEEEEELEEEEEEEEEEEEEEEEELEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8697:5190#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":85,"pairing_fast_score":0.878,"pairing_mismatches":{"(A:02)->(C:34)":96,"(G:34)->(A:02)":25,"(T:02)->(C:34)":103},"score":4279,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
cccgacgatctccgaaggcgtcaaggaacaccgtgtctaacccgagggcatggctagcttgctggtcatcccttgcgtcgcatttatatttaatccacacgactctcggcaacggatatcagatcgtcggg
+
CCBCCCCCCCCCCCCCCCCCCCCELEcdedeeeceed]a^cbcYd^c]T`]aNaV[\_YYYcYad]dZabdebecbbYcXcWccceececEEEEELEEEEEFLEEEEECCCCCCCCCCCCCCCCCCCDACC
@HELIUM_000100422_612GNAAXX:7:1:8635:9276#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":75,"pairing_fast_overlap":86,"pairing_fast_score":0.904,"pairing_mismatches":{"(A:02)->(C:34)":95,"(G:02)->(C:34)":84},"score":3872,"score_norm":0.977,"seq_a_single":22,"seq_ab_match":84,"seq_b_single":22}
ccatcgcgagaggatgacgccaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatctctcgcgatgg
+
CCCCCCCCCC=CC?CCCCCCCC_cac^cY_Zbce_Zdcc`_`KddbcdY`]QJ]\\N^`YY_^`^__J\`beUbb`dbb[e__LBEEEEEAEFFLEEEEEEEAEFEEFCCCCCCCCCCCCCCCCCCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8674:14210#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":103,"pairing_fast_score":0.87,"pairing_mismatches":{"(C:02)->(A:34)":108,"(C:02)->(T:28)":99,"(C:33)->(T:02)":33,"(T:33)->(C:02)":24},"score":2582,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
ccacacgagcaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattcccgcgcgagatgg
+
BBBBBDDDDD3==:=DDDDDDDDKDDDDDDDDK`_a_aOW[SSddYUda]f^\b]Za_MT[``XWaSd`ddT\_aEEEEEBDEEEEEEEEEEEEEECEFEEEEEEEELCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8661:2510#0/1 {"ali_dir":"left","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":79,"pairing_fast_score":0.842,"pairing_mismatches":{"(A:34)->(G:02)":39,"(C:02)->(A:34)":104,"(T:02)->(A:35)":98},"score":1763,"score_norm":0.962,"seq_a_single":29,"seq_ab_match":76,"seq_b_single":29}
cacacgcacggcatgtgccaaggataacaataaatgagaaggactcgtgccgtgtagccccgttcgcggtgtgcatgcggttcgtggactctttgtaattacaaacgactctcggcaacggatatcgtgtgtcgtgg
+
CCDCCCCCCCCCCCCCCCCC?CCCCCCCCEEFEEEDEEL^[WX_VV^a`Hd`__KX\QLHUGVVR_aZUZ\\E>?EBDEE:EEEEAEEDEEEDEEEEMEEEEELEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:8599#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":3946,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
catcacggcgctcgtccgatctccaaccaagcaagaagaccctatagagcttaaccctaaataaataactaacttatcatggattcggttggggcgacccttggttggagatcgcaggagcggttcagca
+
######################EEEEEEEEDEEECEEEEECEEbddbb`dNadccddS]adSb_^_dddbbcd]TUZXT[_V\^Z_bNWYZHVX\WRS[Ob\]Y]T]U?B####################
@HELIUM_000100422_612GNAAXX:7:1:8616:9731#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":85,"pairing_fast_score":0.866,"pairing_mismatches":{"(G:02)->(A:30)":83,"(G:02)->(T:33)":106,"(T:02)->(G:32)":94,"(T:02)->(G:33)":108},"score":2379,"score_norm":0.953,"seq_a_single":23,"seq_ab_match":81,"seq_b_single":23}
ccgacacggcgctcttccgatctccaacaacaatttcaggtcaaggtgcagcctaccgataaaactgtggacgcactcatgaaattggatttggttgtatgttgttggagatcgaaggagcggttcagcag
+
##############A@@C5=<=4_bb^`\O]V^^a[S^VJWRXRQWOQZYXPSYMI\TP\ZYY^a[CE?BCB?D@??ACCCAHEBEEEEBEBBJEEEBEECEEBDKDK#######################
@HELIUM_000100422_612GNAAXX:7:1:8695:4779#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":5858,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacacgacgctcttccgatctccttatgtgcaagaagaccctatagagctttatcacacacctattaatttagacactgattcggttggggcgacccaataaggagatcggaagagcggttcagcagaa
+
B@A===?=A@@=CCCC?C@<8@@CCaceeedbebeeeceeeceefeeeefeceeeeeedaeebeededcedeceeecdeddaaZac__a`_a_aa][[[Z^_b^_dc_A########################
@HELIUM_000100422_612GNAAXX:7:1:8664:1252#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":89,"pairing_fast_score":0.907,"pairing_mismatches":{"(A:02)->(C:34)":97,"(T:34)->(A:10)":33},"score":4150,"score_norm":0.978,"seq_a_single":19,"seq_ab_match":87,"seq_b_single":19}
cctgtaacaattcggttggggcgaccatggaattcatcatccaccacaaataagattatctaatcacccaaaagatcctaacaatagatcaaagaccaagctaccttagggataacagtgttacagg
+
CCCCCCCCCCCCCCCCCCC]_a\^M[\NVcW`Fc_`_abUdedcdcd^c^d_ccVeee]b\c]c_`\^\^eece^Z\W\\baaVaEEEDEEEEEEELEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8838:4641#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:ggttatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggttatt","obimultiplex_reverse_tag":"ggttatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:34)->(g:06)":41,"(g:34)->(c:08)":40},"score":3615,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCCCCCBDCFEEa\`_X`e_\eZVWUVOQLFGda_cd\Y[[\Ybcaab_aY\eecdeedcb]ee_____\\YCCCCCDCCCDCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8685:15027#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":89,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:34)->(G:02)":34,"(C:02)->(A:34)":95,"(C:34)->(G:02)":25,"(G:34)->(A:02)":29},"score":685,"score_norm":0.955,"seq_a_single":19,"seq_ab_match":85,"seq_b_single":19}
cctctgaggattcggttggggcgaccaaggaataaatcatcctacaaaactacgacacatatgtcactccatagaccctaaactaagatcatcaacaaagctaccttagggataacagacttagtgg
+
CCCCCCCBCCCCCCCCCCCEEEEELEEELEEEELE<@<=@A;^N]J]WdXJMODM[>:8885<9;98?<AC>A>9>>A?CA?EAEEEEB@EADBLEBEEEEEEEEEEECCCCCCCCCB@@@@CCACC
@HELIUM_000100422_612GNAAXX:7:1:8675:13788#0/1 {"ali_dir":"left","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":46,"pairing_fast_overlap":79,"pairing_fast_score":0.605,"pairing_mismatches":{"(A:33)->(G:02)":54,"(A:34)->(C:02)":35,"(A:35)->(C:02)":38,"(C:02)->(A:34)":108,"(C:02)->(T:34)":97,"(C:34)->(G:02)":56,"(G:32)->(T:02)":33,"(G:33)->(C:02)":45,"(T:34)->(A:02)":34,"(T:34)->(C:02)":37,"(T:34)->(G:02)":44},"score":1664,"score_norm":0.835,"seq_a_single":29,"seq_ab_match":66,"seq_b_single":29}
ccagcacacagggatgacgccaaggaacaccagtactacctcctgccccgcggagcggtcgtcccgccttccgctcccagggcagcggttacaccttaatcgacacgactctcggcaacggatatcctgtgtgttgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEJLLELMDELEELKDEEEELE=KELDACB>;[O^RVYQZabceb[V[_\UWXXW]^QSYY^^aEDLEEBEEBEEEELCCCBCCCCCCCCCCCCCCCBCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9304:11250#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgatga:gcgatga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgatga","obimultiplex_forward_tag":"gcgatga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgatga","obimultiplex_reverse_tag":"gcgatga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":63,"pairing_fast_score":0.75,"pairing_mismatches":{"(a:02)->(g:34)":76,"(a:14)->(c:02)":42,"(g:02)->(t:34)":80,"(t:02)->(g:16)":63},"score":270,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgcaagaggactactagcaatagcgtaaagctcaaaggacttggcggtgctttatatccct
+
CCDACCCCCCCCCCCCCCLDEEEEEDEB?EE3@9@9981:=8<AA<>CE>BECXLTNIZ<<D:DCCEAE?CEEEEL?CBLECCCCCCBC@CCBC@CCCB
@HELIUM_000100422_612GNAAXX:7:1:8635:16712#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":73,"pairing_fast_score":0.743,"pairing_mismatches":{"(A:27)->(C:02)":64,"(G:25)->(T:02)":62,"(G:29)->(C:02)":73,"(G:34)->(T:02)":47,"(T:02)->(C:33)":101},"score":644,"score_norm":0.932,"seq_a_single":35,"seq_ab_match":68,"seq_b_single":35}
ccatgcagtgcgatatccgttgccgagagtcgtttatggttattgagaggccacgacccatgcacaccccgcgaacgaggcatcacgagacgcgaccttcctatgttttgttttccttggcacatgccgtgcgcactgcatgg
+
CCCCCCCCCCCCCCCCCCACCCCCCCCCCCCBCCCEFEDE@EEEEDLEEEE;EDDCCC-C>C<EAEEAE?ACGC:;<A\WYP\YJ]U`_[D>D:@CC?EDKDEE7DDDCCCCCCCCCCCCCCBCDCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8665:5667#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(A:02)->(G:34)":87},"score":4820,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccatcgactcggggcaatcctgagccaaatccgtgttttgagaaaacaagaaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgagtcgatctgg
+
CCDCCCCC\c^aU][aWSS^Wbdaddddea]eeeeeefeeebbeeeeedddebeaeeeb_eccUcbecec`^^baKaaaEEEEEEELEEEEEEEEEEEEEEEEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8621:6167#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:34)->(A:02)":12},"score":5063,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccactgacgtcccattgagtctctgcacctatccttttttattttcgctttctgaaccttcgtttgttttcggaaaacgtgatttggctcaggattgcccgacgtcagtgg
+
CCCEEEEEEEELEa[^f]ebeeeede`ccc_ecd^bdddbedddd\edd`eefb]cdcdcc^^bcefeddUYW\]cc`__YMZZWaKcEEEEEEEEEEEEEEEEEFFECCC
@HELIUM_000100422_612GNAAXX:7:1:8610:14529#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":103,"pairing_fast_score":0.84,"pairing_mismatches":{"(A:02)->(C:34)":101,"(A:02)->(G:34)":95,"(C:02)->(T:34)":105,"(T:34)->(G:07)":36},"score":4544,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
ccagatcgacgccattgagtctctgcacctatcctttatttattctcgcgttctgaaacccttgtttcttttcataaaacgggatttggctcaggattgccccgtcgatctgg
+
CCCCCbbcRbTT`MN`XX\XXSOV\aVVb`\]]aYGa\]R`_bVeedc_b]bebZIROQJV\]^VbbeeeSYYP_`X[UXTZSO_XaEEEEEEELEEEEELDEELEEEBCCCC
@HELIUM_000100422_612GNAAXX:7:1:9036:17920#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aacctga:aacctga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacctga","obimultiplex_forward_tag":"aacctga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacctga","obimultiplex_reverse_tag":"aacctga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(c:02)->(t:34)":81,"(t:33)->(a:10)":35},"score":3539,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
CCCDCCCCCCCCCCCCCCCELEeddd_\d`Ze[YEX]ZO]c]S]ba`WWWZ[_c`X]ceeaebZedbe^`_]ccebZeDELCCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8683:15230#0/1 {"ali_dir":"right","ali_length":12,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":9,"pairing_fast_overlap":12,"pairing_fast_score":1,"score":887,"score_norm":1,"seq_a_single":96,"seq_ab_match":12,"seq_b_single":96}
cgaaacagataaaagctggggccccggcggtccttgcgggggcgccgtgggctgccgaggccaacagaaaaaaatatgactctcggcaacggatatccagagctctgggatgacgccaaggaacacgataaaagctgaggcaccggcgagcctttcgagggctccgtcggctgccgaggccaacagacaaaaatatgactctcg
+
#################################################C>CCCBCCCBCCCCCBB@BBBCCDCCCCCCCCCACCCCCCCCCCCCCeeeeeeeeeeedCCCCCCCCCCCCCCBCCCCCCCCCCCBCCBDCCAA?B<BA>BA@CCCBA==6;CC@BC:?B:AC@CACCA3@C:3><:?#################
@HELIUM_000100422_612GNAAXX:7:1:8619:16247#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":100,"pairing_fast_score":0.918,"pairing_mismatches":{"(A:02)->(G:34)":98},"score":4590,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
ccacgtgtacgccattgagtctctgcacctatccttttttgattttcagtttctgaacttttatttgtttgttttcggaaagaaggatttggctcaggattgccccgtacacgtgg
+
DCCCCCCCWa]Q_\__bacaec]]_c[^WYad[ebedcab^ceedeeYaeefedeca^bdeedbddcbddddeddEEEEEEEEEELEEEEEEEEEEELEEEEEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9228:6762#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atctctc:tggtggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctctc","obimultiplex_forward_tag":"atctctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtggc","obimultiplex_reverse_tag":"tggtggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(T:32)":36,"(T:02)->(G:30)":54},"score":405,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagaggactacgagcaatagcttaaaactcaaaggacttggcggtgctttatatcccg
+
CCC?CCCACCCCBA>AA<7:=:9EE`WSP\\R^AAJ:>7>>:;9<=/22--29HA2=CCAB>A>A@A;7=:8>HA=H<>@@B?CCCC7@CCCA<@>@<:(
@HELIUM_000100422_612GNAAXX:7:1:8615:14601#0/1 {"ali_dir":"right","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":79,"pairing_fast_score":1,"score":4934,"score_norm":1,"seq_a_single":29,"seq_ab_match":79,"seq_b_single":29}
tctttccctacacgacgctcttccgatctccacgtacgctccattgagtctctgcacctatctcgtcgtagaaacaagatttggctcaggattgcccactgtgcgtggagatcggaagagcggtgcagcaggaatgc
+
###############CACCAB@CC=C@CA\]\caddad_]_cTeeceeeeedeee`eeeedeeebeefdeeeeeddeedbeeeecbeefeeecd`cZcEEEEEEEEEE#############################
@HELIUM_000100422_612GNAAXX:7:1:8645:11006#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":5338,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
ccgatctccagagctctggggcaatcctgagccaaatcctgttttacgagcaattcaaaaaggaatcaaaaataggataggtgcagagactcaatggcagagctctggagatcgg
+
####C>A_Z]L`JYa\[ZRMPRRRRROQ_]_ZcYceedbde]baedededaeecec^eee`eeeeZ\deeeee`^_d__\_`c_]_cb``eEEECEEEEEEEEEEEEE#######
@HELIUM_000100422_612GNAAXX:7:1:8628:14219#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":105},"score":5223,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccagtcgagctgggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggagctcgactgg
+
CCCCCCCCZbeebeeeb[T^[]eb`ecedddbeeeceeebd`dddedddd_deeeeccee^bdc`__a__eccceYaRa`aaaEEEEEEEEEEEEEEEEEEEEELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8667:5013#0/1 {"ali_dir":"left","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":91,"pairing_fast_score":1,"score":4062,"score_norm":1,"seq_a_single":17,"seq_ab_match":91,"seq_b_single":17}
cctgtaacactgttatccctaaggtagcttatgtttgtagtcttggtagggtctgttgattgattaattgatctttattatgagggatgatttatcctaggtcgccccaaccgaattgttacagg
+
CCCCCCCCCCCCCCCCCEEEEEEEEe\accebeeeeedcdZa__I_[Y\M^__caZd^[cedcceebebdYYYZZeedeec_cccEEEEEEEEEEEFEEEEDEEEEEECCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9058:7151#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (taccgtg:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taccgtg","obimultiplex_forward_tag":"taccgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:02)->(g:34)":76},"score":2769,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCBCCCAELELEEFEEc_cVYHV]\`cdZYZVZVWZXSV`_[U\\\_VXW`^ebcba\_a`cELEEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8699:20789#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":106,"pairing_fast_score":0.883,"pairing_mismatches":{"(A:02)->(G:34)":90,"(A:02)->(T:34)":102,"(T:02)->(C:34)":97},"score":5269,"score_norm":0.972,"seq_a_single":2,"seq_ab_match":103,"seq_b_single":2}
ctccacgtactgcccattgagtctctgcacctatccttttttcgccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgcccgcagtacgtggag
+
ABaQRRRRRZc]_ZYZ\Y[^Y\]cccbececceebeecededebceeee^eee]cdebaac`deeddddNebaUc``\[\[\dEEEEEELEEEEEELEEEELEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8676:12262#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":105,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(T:21)":82,"(A:07)->(G:15)":54,"(C:34)->(A:02)":12,"(T:02)->(G:34)":87,"(T:34)->(G:02)":15},"score":3719,"score_norm":0.952,"seq_a_single":3,"seq_ab_match":100,"seq_b_single":3}
ccgcgtacgctccattgagtctctgcacctatccttttttattttcgctttctgaacctttgtttgtttttggaaaacgtgttttggctcaggattgcccgatacgcgtgg
+
CC6EEEEEEEELEELEEeUeb]YYSX]]`[^cUd_`dddec\\`\ded^eca]4Y[bc_^ad_aZbedba`[deeBEDEEE?EEEELDEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:9080:13956#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3888,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
gataccgcgaccaccgagatctacactctttccctacacgacgctcttccgatctccacggtacttagataccccactatgctagaggagcctgttctattgttgtggagatcggaagagcggttcagcaggaatgcggagaccgatatcgtatgcggtcttc
+
####?'??A,,??><<?*6=A==CACCCC@BCCCCACBCBCCCCCCCCBCCCCCCeedeeeeeeeefededeeeeeeeeeeedeeeeeeeeeeeedecedXedeeeedCCC@3C:CBCCCCAACC######################################
@HELIUM_000100422_612GNAAXX:7:1:8699:14495#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":2669,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCDCCCLBEEEEEEEeZYeQYXX\VOY`UO\VY\MQ\aGW_[YK_XbXW]b`bSb`c`U_aEEEDEBEBCCCCCCBCCCCCC@CCCC
@HELIUM_000100422_612GNAAXX:7:1:9117:7388#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acacgta:acacgta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acacgta","obimultiplex_forward_tag":"acacgta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacgta","obimultiplex_reverse_tag":"acacgta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(c:20)->(a:02)":42,"(t:25)->(g:02)":39},"score":892,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
BB@ABBBB?BBBBBBB<BBCEDBDBD><CCC>C4;@:@CCC>CDDDB`TZJU[LRZZ]b_Y]]EDEEEBEEEEEEEEBEEECCCCCCAA>=>ACCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8628:2763#0/1 {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4246,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
aacaccgagatctacactctttccctacaccacgctcttccgatctcccacagagaggatatccgttgccgagagtcatattttttctgttgccttggccttggcaacagatcgcaagagcggtccagcagaaatgcggagacagacatagtat
+
######################################@@C;:BCCceefeedeeeeeeedeceacebeebeece_e_bbddeeddddcceeaWeecU_`a`]IU\aE##############################################
@HELIUM_000100422_612GNAAXX:7:1:8650:12048#0/1 {"ali_dir":"left","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":82,"pairing_fast_score":0.848,"pairing_mismatches":{"(C:34)->(G:02)":47,"(G:34)->(T:02)":41,"(T:34)->(A:02)":33},"score":2757,"score_norm":0.963,"seq_a_single":26,"seq_ab_match":79,"seq_b_single":26}
ccactgctgcaggatgacgccaaggaacacgataaaagctgaggcaccggcgagccgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatctgcagcagtg
+
CCCCCCCCCCCCCDCCCCCCCCCCCCEEEEEELEEEEEDELEEEEELEEcca[]\Pedbddeea_eabcad`^bccc_\c`cbdcaKc\aEEDDDDDEEEEEEDEEEECCCCCBCCDCCCCCCCDCCBCCDC>C
@HELIUM_000100422_612GNAAXX:7:1:8672:4138#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(G:34)->(T:07)":40},"score":4963,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccacactgtcgccattgagtctctgcacctatcatttttgatttttgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgccccgacagtgtgg
+
CCCc_cUR\XI^Z^cd^ddde^aaX`\Ubccec^dddddGaeeeeeeaeeeedec^eddebbbd_deedb[_addccEEEEEEEEEEFEEEEEFEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8623:2950#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":4743,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctactgcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccctgctcagcag
+
##EEEEa_R[][_]^]eeaa\[`Y_`_`\L]IYXe]eb^ee`ccdeeebacaabe[ddeedd`beaeebdedbbccaDEEECEEEEEEEEEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:9028:8263#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gccggcg:tctgagg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccggcg","obimultiplex_forward_tag":"gccggcg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tctgagg","obimultiplex_reverse_tag":"tctgagg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(C:02)->(G:21)":49},"score":1970,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggagtactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
DCCCCCCCBCCCCCDCCCR^Xaebcd]e\\ec_b^bcbcc_`^]^UVX?83DEE=E?EEFEEEFDEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8636:6606#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":89,"pairing_fast_score":0.907,"pairing_mismatches":{"(A:02)->(C:34)":99,"(T:02)->(A:34)":95},"score":2978,"score_norm":0.978,"seq_a_single":19,"seq_ab_match":87,"seq_b_single":19}
ccttcgccactgttatccctaaggtagcttggtctttgatctattgttaggatcttttgagtgattagataatcttatttgtggtggatgatgaattccatggtcgccccaaccgaattggcgaagg
+
CCCCCCCCCCCCCCCCCCCUVaL]]XXZZaQaabebbeee_d___cZec[dXeceeeeee_]aVaEEEEEEEEEEEEAEEEEEEEEEEEEEEFELEEELEEEEEEEEDCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8612:13017#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4337,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctacactctttccctacacgacgctcttccgatctccaacgagcccgtatatacatacgtatacgtctgaatgaaagagttggtgtatggctggagatcggaagagcggttagcaggaatgccgagaccgatatcgtatgcgg
+
#########?;;=C??@C@AAB<ACCCC=>AAA??B@?ACAA@B=CCCCdedeeeefeeededeeeeeeeeedeeeeeeeeeeedeeedeeeeeaeeeecebeeeacaCCCC>C<C<CCACC0C6??9=A=A;=:>-<?BA@###############
@HELIUM_000100422_612GNAAXX:7:1:8631:16853#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:32)->(G:02)":14,"(T:02)->(G:34)":103},"score":4275,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccacgtagtcgggacaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgactacgtgg
+
CCCCCCEEEEEEEJEEEECEEaee```aZcdee_ec_ebccPX]bade_Zdaed_]cebbcec_eX^^a]c`\^d\_cc`e]beEEEEEEEEEEEEEEEEEELEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8618:9756#0/1 {"ali_dir":"left","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":93,"pairing_fast_score":0.822,"pairing_mismatches":{"(A:02)->(T:31)":106,"(C:08)->(A:34)":58,"(C:28)->(G:02)":25,"(G:34)->(A:02)":29,"(T:34)->(G:02)":16},"score":2962,"score_norm":0.946,"seq_a_single":15,"seq_ab_match":88,"seq_b_single":15}
ccgcctgtaattcggttggggcgacctaggataaatcatcctaataaactaagactaatatgtcactccatagaccctaaactaagatcatcaacaaagctaccttagggataacagtacagg
+
CCCCCCC?CCCCCCBLADEAB@BBFEEEL=EAEaYYNY]a\XZ^XFUS]^W]\Y\a\F^[]W[U\W]BcZYX[\Ld]^a`YUSXYaE@EE?EEEEBEED?@E@EEIDECCCCCCCBCCB?CCC
@HELIUM_000100422_612GNAAXX:7:1:8660:19367#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5986,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccgatctccaacaacatacaaccaaatccaatttcaattaacctgcccgttcttctgtttgctgctgcaccttgacctgaaattgttgttggagatcggaagagcggttcagcag
+
##########?==CACC?CC=ACeeeeeeeebebeZebeee]eeedeeeeedeeeeeeeeeaeeeeeedeeeeeeee]^c]^c_ccccee`ae[Z[\]dbdcc]^]]UA######################
@HELIUM_000100422_612GNAAXX:7:1:8685:13702#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":106,"pairing_fast_score":0.951,"pairing_mismatches":{"(C:02)->(A:34)":94,"(T:02)->(G:34)":108},"score":6096,"score_norm":0.981,"seq_a_single":2,"seq_ab_match":104,"seq_b_single":2}
ctccactgcagtggggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggcactgcagtggag
+
?C\ccc`ccd_`ceeabe`eeb]edebebdebeeee]eeeZddddeedcdddeeddefeedeeeeebcbdbdeed[d]]\WZa^]]]_cEEEELEEEEEEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:8700:15340#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":85,"pairing_fast_score":0.951,"pairing_mismatches":{"(G:34)->(C:05)":35},"score":5714,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
cctacacgacgctcttcctatctccactcgctacgggcaatcctgagccaaatccttaagttaaaatcaaaaaaggataggtgcagagactcaatgggtagcgagtggagatcggaagagcggttagcaga
+
########ABCCC;,?9<);AAAZecbecdd^ccHc__acaY\aeeebeeeeececeeeeddeeefedbeeeeeeebe]ccccUacccbb]bb_`__aRYXYY][X]V??=3+-(000C?<@#########
@HELIUM_000100422_612GNAAXX:7:1:8652:3041#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":73,"pairing_fast_score":0.829,"pairing_mismatches":{"(C:02)->(A:34)":85,"(C:02)->(T:33)":99,"(T:34)->(G:02)":40},"score":1117,"score_norm":0.959,"seq_a_single":35,"seq_ab_match":70,"seq_b_single":35}
ccactgcagtggatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcgtttattgttttccttggcacatgccgtgcgtacgacgtgg
+
CCCCCCCCACCCCCCCCCCCCCCCCCCCCCCBCBCEBB:L56;:;?AC?+YRaZNRRIW\^]^ORLWLSFaXBBBBE>BEEAEELAEEE5EBEEAEE:KEADEEEE<@B?@CCBBBB??<B>B@?5CCCCC@CCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9275:3571#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgacac:gcgacac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgacac","obimultiplex_forward_tag":"gcgacac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgacac","obimultiplex_reverse_tag":"gcgacac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(C:33)->(T:02)":22,"(T:34)->(A:02)":25},"score":805,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
CCCBCCCCCCCCACCCCCEEEKDELCbe_^ecebcZSeEEBEEEEFECEEEBCE@ECEEAEDEEEEDEDEEEEEELBEELDCCCCCCCCCCCCCCCCCB
@HELIUM_000100422_612GNAAXX:7:1:9395:3591#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgaacg:gcgaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgaacg","obimultiplex_forward_tag":"gcgaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgaacg","obimultiplex_reverse_tag":"gcgaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":36,"pairing_fast_overlap":62,"pairing_fast_score":0.61,"pairing_mismatches":{"(A:02)->(C:33)":72,"(A:20)->(C:32)":54,"(A:34)->(G:02)":23,"(A:34)->(G:07)":37,"(T:29)->(G:14)":35},"score":1556,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
?9?ACCCBCCCCCBCCC@CBE=LEEc[Pb_[cRE@NGcb_eba^]_]RSVSJ_B[YRYCC?@?EEEEEEE=KEEEDLE?ELA>CC>CCCCCACC0CCCC>
@HELIUM_000100422_612GNAAXX:7:1:8720:19249#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtaatta:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtaatta","obimultiplex_forward_tag":"gtaatta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":875,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCDCCCCBCCCCBCCBCLKEDEEEDEEEEEEDDBDCEAEE/EEDEDEE>=EWU^TYc[`[R_WYQbBEEEEEEEEAEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:17657#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":86,"pairing_fast_score":0.952,"pairing_mismatches":{"(T:33)->(G:08)":30},"score":5880,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ctacacgacgctcttgcgatctccgaggcttggtcgccccaaccgaatttaatttttacttatttagtaggttaaattaagctctatagggtcttcttgaagcctcggagatcggaagagcggttcagaa
+
:3<CCC;C:9<<<+6'8*CCC>dddddddE]`aYaa^b`cZb^Ydddddbddddddd_dddddddddbdddddddbdcdWc^ddddddd]db``Y``b`bbbbbb^`P@@@###################
@HELIUM_000100422_612GNAAXX:7:1:8724:3507#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tatcagc:tatcagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatcagc","obimultiplex_forward_tag":"tatcagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatcagc","obimultiplex_reverse_tag":"tatcagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":780,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCBCCCCCCCBCCCCBCEEEECEEEEEEEEE][Pd`\WV]SQUa<E=AEDBECABE<E?ABEEBEEBC?EDDDDAABELC@?CC@C8CCCC@?CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:12684#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":3323,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacacgacgctcttccgatctccggtgagaggtcgccccaaccgaatctttgatcggtttttcgattgtttgataaagctctatagggtcttcttgtctcaccggagatcggaagagcgcttcagcaga
+
########################EEEEECEEEEB<BB^XO\Scc`cbc`cZe`]`Sb]aR\^^aQ]VaceXed]debbXXcedaeebbEEEEECEDEEEEEEEEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8611:5303#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":87},"score":3864,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccactgtgctggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagcacagtgg
+
CCCCCCEEEEEEEEEEEEEEEEEEEaa_adTbdd]\X]Yeabdeeeedbcbee]cedea^eabad_daaa__aZc_cc`caEEEEELEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8641:20681#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":85,"pairing_fast_score":0.951,"pairing_mismatches":{"(G:33)->(T:02)":32},"score":4006,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
ccccacacggcgctttccgatctccttcaacgcaagaagaccctatagagcttcattttactcactacatatattgaacaaaattcggttggggcgacccgttgaaggagatcggaagagcggttcagcag
+
#######################EEEEEEEEKEEEEEEcRc]^edeeeedece]ecece]eedeefddeddeddbd^ed`cc[]^accX[YVWccdEEEEEEEEFEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:9295:1835#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtaaca:tgtaaca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtaaca","obimultiplex_forward_tag":"tgtaaca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaaca","obimultiplex_reverse_tag":"tgtaaca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":3898,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattctataacaaaataattcgccagagaactactagcaacagcttaaaactcaaaggacttggcggtgctttatacccct
+
CCCCCCCCCCCCCCCCCCLEEEeadb\eZ\e[`ZY]ba`c]b_dada_c_bd]RZ[ZZeda[e^bdcce`beWe_ZZebZcCDCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8935:14910#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3917,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
gatacggcggccaccgagatctacactctttccctacacgacgctcttccgatctccactaagttagaacaggctcctctagcatagtggggtatctaaacttagtggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgcggtcttc
+
####?0A?5/(?:=A0AAA=AA.B@AABA?C?C=>7>6C=@C@CCCCC?DBCCCCecceeeeeeeeeedeeeeeeeeeeeeeeeeeeeeedeeeeeeeeeeeeefeeeACA@AACCCCCCBBCCC=:=8=A?AA@=?;?=AAA####################
@HELIUM_000100422_612GNAAXX:7:1:9165:14015#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tatgttg:tatgttg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatgttg","obimultiplex_forward_tag":"tatgttg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatgttg","obimultiplex_reverse_tag":"tatgttg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(G:34)":56},"score":1570,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCcPccbcdd_dd_dd[QZaZ\[][aEBEEEBEE>EEBLE9EEBEECEE8EEEEEEEEELCEBECCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8672:15366#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":89,"pairing_fast_score":0.791,"pairing_mismatches":{"(A:02)->(C:34)":105,"(C:02)->(A:34)":99,"(C:31)->(A:02)":25,"(G:33)->(T:02)":29},"score":3238,"score_norm":0.944,"seq_a_single":19,"seq_ab_match":84,"seq_b_single":19}
ccttcgccaattcggttggggcgaccatggaattcatcatccaccacaaataagattatctaatcactcaaaagatcctaacaatagatcaaagaccaagctaccttagggataacagtggcgaagg
+
CCCCCCCCCCCCC?CCCCCEEEEEIEEEKEEaTXYYY_XQ\aeWeK^\[\a^aL_\_ca`^Rc`_]\\]Tfedeb\]`]\a`O`bEEEEEEEEEEFLELEEEEELEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8655:1134#0/1 {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4454,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
cccaccgagatctacactctttccctacacgacgctcttccgatctccagagctctgggatgacgccaaggaacacggatttggctcaggattgccccgactctctggagatcggaagagcggttcagcaggaatgccgcgacagatatcgtat
+
##############################?0A:;+::8:A-,ACA_eccXebUcYdeebeedeeeeaedeeeeceeeeeeeeeeeeeeeebc^eceeecceeeeeeb;CB9A?A??@BBBAA###############################
@HELIUM_000100422_612GNAAXX:7:1:8616:16594#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":92,"pairing_fast_score":0.798,"pairing_mismatches":{"(G:02)->(C:29)":102,"(G:20)->(T:02)":47,"(G:29)->(T:02)":20,"(T:02)->(A:25)":92,"(T:02)->(G:33)":100},"score":434,"score_norm":0.946,"seq_a_single":16,"seq_ab_match":87,"seq_b_single":16}
ccggcgctgttatccctaaggtagcttggtctttgatctattgttaggatcttttgagtgattagataatcttatttgtggtggatgatgaattccatggtcgccccaaccgaatcgccggcgg
+
AAAA@AAA@@>8;)A1@@<G=8@@@@:;5@CC<CC0=;@7D5@8=:>-::D=@;BB6:6`XOLTX^a<C::4@8:6=65:>-8>CB<B<9@C08BB<B:K4GD@06>40885888A3A@AAAAA
@HELIUM_000100422_612GNAAXX:7:1:8745:15899#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcggtc:ttcggtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcggtc","obimultiplex_forward_tag":"ttcggtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcggtc","obimultiplex_reverse_tag":"ttcggtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"score":3274,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
CCCCCCCCDCDCCCCCCCEBEEee__eecbeQNSWZXaUaIV_XbX]QRYPb[\UZ^Ua^X^__V`Q]cc_b\`_K^EEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9313:2031#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acaccgt:acaccgt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acaccgt","obimultiplex_forward_tag":"acaccgt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaccgt","obimultiplex_reverse_tag":"acaccgt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(a:02)->(g:34)":76,"(c:02)->(g:34)":55,"(c:18)->(a:20)":45},"score":1374,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCBCCCAACC@J8ELEEEEEa^WIa`@^a\Ha\[[\_6[STa]_Va=LBAEEEDB>CADDBEBECCEELEE;EECCCCCCCAACCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9388:5910#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gccgtgc:gccgtgc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccgtgc","obimultiplex_forward_tag":"gccgtgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccgtgc","obimultiplex_reverse_tag":"gccgtgc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3142,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CBCBCCCCCCCCCCCCCCCEEEEDFFEEEaP`MUWYZZP^]MY\Y\YZZ_dYebbaVc^`]WUaM^d^_ccUccc`T`__\CCCCCC?CCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8935:16153#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (taccgtg:taccgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taccgtg","obimultiplex_forward_tag":"taccgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taccgtg","obimultiplex_reverse_tag":"taccgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:02)->(g:34)":76},"score":2208,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCACCCCCCCCCCCCCCCELELEEEEe__eYYWVYV[_Z_^Y^[[YYa_]ZeXUYWHYTSRZ[YaEEEEEEEEELCDCEECCCCCDCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8662:14787#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":104,"pairing_fast_score":0.812,"pairing_mismatches":{"(C:02)->(G:34)":107,"(G:02)->(C:34)":108,"(G:02)->(T:34)":81,"(G:06)->(A:34)":56,"(G:23)->(C:34)":49,"(G:34)->(A:02)":17,"(G:34)->(C:02)":5},"score":2361,"score_norm":0.933,"seq_a_single":4,"seq_ab_match":97,"seq_b_single":4}
ccacgctagctccattgagtctctgcacctatcttttttttcttttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgcccagctagcgtgg
+
CCCCLEEEEEEEEDEELEEEEEEEEEEEEEEEEddd_cc^bd`dd]MZCcRQPOJGRRQQeebXdUM\`W]V_ZccccDELEEEEEEEEEEEEEEEEEEEEEEEEELLCCCC
@HELIUM_000100422_612GNAAXX:7:1:8652:12588#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(A:02)->(C:34)":105,"(C:02)->(T:34)":107},"score":3506,"score_norm":0.978,"seq_a_single":18,"seq_ab_match":88,"seq_b_single":18}
ccaatgtcctacaaccaaatccaatttcatattaatattaaaatcaacaatccaataaacaaataacaatgtaatccatcattccacttagaaacaagctgcaccttgacctgaaatggacattgg
+
CCCCCCCCCCCCCCCCCC\T[`X_Z_a]eeeXedeeebdeeeeebdeecec^a]_Qcee]Yb]]`]dcdcEEEEB?EEDEDFEEEEEEEEEEEEEEEEEEEECELELECCCCCCCCCCCCCCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8687:9284#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":85,"pairing_fast_score":0.72,"pairing_mismatches":{"(C:02)->(A:34)":102,"(C:02)->(T:34)":94,"(C:10)->(T:16)":53,"(C:34)->(G:02)":36,"(G:02)->(T:35)":79,"(G:34)->(A:02)":25,"(T:02)->(A:34)":93},"score":2459,"score_norm":0.918,"seq_a_single":23,"seq_ab_match":78,"seq_b_single":23}
cccacgctgagccgaaggcgtcaaggaacactgtgcctaacccgggggcatgtctagcttgctagccgtccctcgtgttgcaaagctatttaatccacacgactctcggcaacggatatcctcagcgtggg
+
CCCCCCCCCCC;CCCCCCACCCCELEEEEEEDFEELa^bbbbeded^\YULa4^]ZFXZVSb]`_V]b[bcebc]ZaEM?EEEEDEEEEEEELLEEDEEEELEEEEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8670:14285#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":106,"pairing_fast_score":0.951,"pairing_mismatches":{"(A:02)->(G:34)":90,"(T:02)->(G:34)":108},"score":5552,"score_norm":0.981,"seq_a_single":2,"seq_ab_match":104,"seq_b_single":2}
ctccacgcactagccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgcccctagtgcgtggag
+
A;aeed^e\cc__W\Y\Y^`_cecc_bd_`___bce_bacecebdeeeebfce_cddfdcdcdeedbcd`eadad`cU\Z\XaEEEEEELEEEEEEEEEEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:9355:15997#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgcatt:gcgcatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgcatt","obimultiplex_forward_tag":"gcgcatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcatt","obimultiplex_reverse_tag":"gcgcatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(t:34)->(c:22)":38},"score":3711,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCACaCabeeedYcde_XYOU[C[^bE\]_bZ][_]aa`c]c[]^WW^`PcZcaecc``ccZ``_UCCCCCCCBCCCBC@BCCCC
@HELIUM_000100422_612GNAAXX:7:1:9143:4360#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(g:02)->(t:34)":80},"score":2365,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaacagcttaaaactcaaaggacttggcggtgctttatacccct
+
CCCCCCCCCCCCCBCCCCEELEEEEEEEFEEEBDEDDCbbYVbebea_]__c`aac`cc[YZcd`cdcae]cbEDEEEELECCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8651:3776#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5705,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccacgagacgtccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgcccacgtctcgtggag
+
CCb^`YYaNRRORM\_[^abceecc``ca_eeeeeefe[eece_eeeedeeeeeddeedddcdfeddddeda^^a\\T^]a`eeZecEEEEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8684:4650#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":48,"pairing_fast_overlap":73,"pairing_fast_score":0.686,"pairing_mismatches":{"(A:02)->(C:34)":93,"(A:28)->(G:02)":39,"(C:02)->(T:34)":95,"(G:02)->(A:34)":87,"(G:28)->(T:02)":49,"(G:34)->(T:02)":38,"(T:02)->(A:34)":103},"score":944,"score_norm":0.89,"seq_a_single":35,"seq_ab_match":65,"seq_b_single":35}
cccacagacgtgatatccgttgccgagagtcgtttgtgatttcaaggaggccgcatcctgtatgcacaccgcgaacgggacaaagcaggatgcatccttctaatatgttgttttccttggcacatgccgtgcacgtctgtggg
+
CCCCCCCCCC?CCCCCCCCCCCCCCCCCCAC@CC=L@LFDC?CCBC@BFXRZYXT^TaZ[[]QCKLOL[ADBCBEC>C?:AC:DEELEEECEL?LEEEEEEBLEEFDDC?CCCCCCCCCCCCCCCCCCCDCCBCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9227:19029#0/1_sub[78..84] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tccggcg:gccggcg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tccggcg","obimultiplex_forward_tag":"tccggcg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccggcg","obimultiplex_reverse_tag":"gccggcg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"score":4045,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
cacgtac
+
edcde`e
@HELIUM_000100422_612GNAAXX:7:1:9090:10224#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ggtctgg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggtctgg","obimultiplex_reverse_tag":"ggtctgg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"score":3107,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
CCCDCCCCCCCCCCCCCCEDEEEFEEDEccY\]ZZYaS\a__^Y]^YWXZWccca_a^c`\V_\c_dcdba]Z^_aDDEEFCCCCCBDCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:102:18053:1635#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gactcct:gcctcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gactcct","obimultiplex_forward_tag":"gactcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":58,"pairing_fast_score":0.855,"pairing_mismatches":{},"score":2426,"score_norm":0.966,"seq_a_single":50,"seq_ab_match":56,"seq_b_single":50}
tttt
+
X[MT
@HELIUM_000100422_612GNAAXX:7:1:9075:15078#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttatttg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatttg","obimultiplex_forward_tag":"ttatttg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(T:34)->(G:02)":21},"score":560,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
@CCCCCC@CCCC@CCCCCACLEEECBEC;;;8;775]NZ\VSKYY_C;==7;:AEACDDADECEEAEEEDEDELEEIB=ELCACCCCB@BCCBB>CCCC<
@HELIUM_000100422_612GNAAXX:7:1:9340:14793#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4331,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
agagg
+
eeeee
@HELIUM_000100422_612GNAAXX:7:1:8699:7721#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5164,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacagctcgaccattgagtctctgcacctatccttttttattttcgctttctgaacctttgcttgttttcggaaaacgtgatttggctcaggattgccccgcagatgtgg
+
CCCEEEEaa][Ma__accUccabXX[a]U`^dLXVU__eecbddab]beebcbebcdbae^\X`^ccee^deabceec]c^Y^O^aEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:9183:14947#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtattc:ttcggtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtattc","obimultiplex_forward_tag":"tgtattc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcggtc","obimultiplex_reverse_tag":"ttcggtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(A:33)->(T:02)":19},"score":942,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCBCCCCDCACBCKEEEedb_Teb[dbcdMdEEDEBEEEEECCC@=EA?CEACDD7CEEEEDEFEFEEEELBEELECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8782:1469#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:06)->(a:34)":59},"score":3205,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgcccaagaactactagcaacagcttaaaactcaaaggacttggtggtgctttctacccct
+
CCCCCCCCCCCCCCCCC=CaL]Pb\a\Ve\[c^a\bd^_bebYc\Y_e[bZ\_a[Y__GNUX]XUZR[\`dVdEEEEEEBECCCCCCCCC?CCC=CCACC
@HELIUM_000100422_612GNAAXX:7:1:8690:2510#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":46,"pairing_fast_overlap":85,"pairing_fast_score":0.561,"pairing_mismatches":{"(A:02)->(C:34)":97,"(A:02)->(T:34)":102,"(A:28)->(G:08)":59,"(A:29)->(G:05)":56,"(A:34)->(G:02)":26,"(C:02)->(T:33)":84,"(C:34)->(G:02)":24,"(G:34)->(T:02)":32,"(T:02)->(C:34)":107,"(T:31)->(G:23)":50},"score":1231,"score_norm":0.871,"seq_a_single":23,"seq_ab_match":74,"seq_b_single":23}
ccacgcacgtagatatccgttgccgagagtcgtgtggattaaatagcattgcaacacgaggggcagctagcaagccagccgcttccccgggttaggcacagtgttccttgacgccttcggtacgtgcgtgg
+
CCCCCCCDCCCCCCCCCCCCCCCLDLDEBCELDEFa_XZ_c^WHYUZ\b@ZTO>MCLG@]]WYP[?DA=BEECDDLEDBEEEEKEEEEEEEEFEEELEEEELEDEELECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:41:9892:3024#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":49,"pairing_fast_overlap":61,"pairing_fast_score":0.845,"pairing_mismatches":{"(C:07)->(A:33)":94,"(T:02)->(A:34)":108,"(T:34)->(G:17)":55},"score":3467,"score_norm":0.951,"seq_a_single":47,"seq_ab_match":58,"seq_b_single":47}
ccgcctccttagaacagggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC?CCCCACe_b[WaaD_cbea^``e_`X]X`aa^d_c\__\U]_\_^TcccY\YF\U^[^\SUa\aEELC@ACCCCCCCCCCCCCCBCCCCCCCC;CCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:16669#0/1 {"ali_dir":"right","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(G:34)->(T:09)":39,"(T:02)->(C:34)":104},"score":3810,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
cgatctcccgactgagcgggcaatcctgagccaaatccggttcgtggagacaatggtttcttctcctagcataggaaggggataggtgcagagactcaatgggctcagagatcg
+
######EEEFEEEEEEEEEEEEEEDEEEEEEaVdad\dFbcdeafaeeef`eee]eeeeeeeeeeZ[\[NZZYZYYKZZ]SORQQR`^UNaacaEEEEEEEEELEEEE######
@HELIUM_000100422_612GNAAXX:7:73:1526:5308#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aaataac:aattaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaataac","obimultiplex_forward_tag":"aaataac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":59,"pairing_fast_score":0.929,"pairing_mismatches":{},"score":4200,"score_norm":0.983,"seq_a_single":49,"seq_ab_match":58,"seq_b_single":49}
caata
+
eeeee
@HELIUM_000100422_612GNAAXX:7:1:8649:21142#0/1 {"ali_dir":"right","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":98,"pairing_fast_score":1,"score":4924,"score_norm":1,"seq_a_single":10,"seq_ab_match":98,"seq_b_single":10}
cttccgatctccactgcacgtgggcaatcctgagccaaatcctgaaacaaataaaagttcagaaagtgaaaataggataggtgcagagactcaatggacgtgcagtggagatcggaag
+
=A?8==A><2N]]XXJYZcacc_cccc_`c_K`Lbe^eecbe]ddeebddccedcbc\ZbX^\[Wc\c_cddd^cX]][\`a^NY^aEEEEEEEEEEEEEEEEFEFEE##########
@HELIUM_000100422_612GNAAXX:7:1:8701:7646#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":102,"pairing_fast_score":0.828,"pairing_mismatches":{"(A:02)->(C:34)":107,"(A:02)->(G:34)":82,"(C:02)->(A:34)":100,"(T:02)->(G:34)":103,"(T:34)->(G:17)":37},"score":4376,"score_norm":0.951,"seq_a_single":6,"seq_ab_match":97,"seq_b_single":6}
ccatgctgctggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagcagcatgg
+
CCCCCC`_d_c_]c_W__]_ZJccMbece_c_cbcXDa\deeZeeebd]IbeedbeeeKcbe^`bbcecb__aVaEEEEEELEEEEEEEEEEEEEEEEELEELEFELDCCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8981:16633#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gatctac:gatctac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatctac","obimultiplex_forward_tag":"gatctac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatctac","obimultiplex_reverse_tag":"gatctac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(a:08)->(t:32)":65,"(c:02)->(a:24)":69,"(t:26)->(a:02)":35},"score":200,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
34:BBBAA??AAABBBABBBI4C/=AAAD>BDB4D;A>=A53-:=6;4A>>>:0A73A=C9AS[DN]BBECE>??C?=EBE?CCCCCA7CCCBCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8643:8379#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":105,"pairing_fast_score":0.794,"pairing_mismatches":{"(C:02)->(A:28)":97,"(C:02)->(A:33)":104,"(C:17)->(A:02)":19,"(G:30)->(T:02)":14,"(G:34)->(T:02)":12,"(T:02)->(G:34)":100},"score":2565,"score_norm":0.943,"seq_a_single":3,"seq_ab_match":99,"seq_b_single":3}
ccacgcatcgagggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggtcgatgcgtgg
+
CCCEBEB<EEELEHEEEB;EEBeeT]bedV^WU[NDUNNYRZW`X[NZW]UPGL\cYbUb[^]U^SY_X<EDEADDEAEAAED<<EEDEDEEEAEEFDBLEEEKEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8613:15757#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":87,"pairing_fast_score":0.655,"pairing_mismatches":{"(A:02)->(C:34)":98,"(A:34)->(T:02)":37,"(C:02)->(G:34)":99,"(C:02)->(T:34)":102,"(G:02)->(T:08)":78,"(G:25)->(T:02)":31,"(G:29)->(T:02)":54,"(T:02)->(A:34)":91,"(T:34)->(G:02)":36},"score":564,"score_norm":0.897,"seq_a_single":21,"seq_ab_match":78,"seq_b_single":21}
acagacgcaccgaaggcgtcaaggaacactgtgcctaacacggggacgtggctggcttgctggccgcaccccgtgtttcaatgctatataatccacacgactctcggcaacggatatctgcgtctgtgg
+
CCCCCCCCCCBC@CAACCACCEEEEEEEEDC99>>LLDEE@ECCCEDE>A>C8G78C=@>978[OZS]\PO]JP_CE2EEEEDEEEEEDEL2EE8BELLEELEBEEEECCCC=CCCCCDCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8693:18485#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":107,"pairing_fast_score":0.856,"pairing_mismatches":{"(C:34)->(A:02)":12,"(G:02)->(C:34)":97,"(T:02)->(G:34)":85,"(T:34)->(G:02)":15},"score":2600,"score_norm":0.963,"seq_a_single":1,"seq_ab_match":103,"seq_b_single":1}
cccatcgctcaccattgagtctctgcacctatccctcttttctcgctttctaaactctagtttgttcgcgtaaaccaggatttggctcaggattgcccgcagcgctggg
+
CEEEEEEEEEELEELEEEEEEEEEEEEEEFEc_\cccI`Xbeb]cedbdec]]^cee``cdeeeeae`bEDEEEEEEEEEEEEELEEEEEEEEEEELEEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8644:6751#0/1 {"ali_dir":"right","ali_length":77,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":77,"pairing_fast_score":1,"score":4470,"score_norm":1,"seq_a_single":31,"seq_ab_match":77,"seq_b_single":31}
acgctttccctgcccgacgctcttccgatctccagacgtacgccattgagtctctgcacctatcccgtacgtaacggggcttggctcaggattgccccgtacgtctggagatcggaagagcggttcagcaggaatgccg
+
###############################EEEEEEcc[d^^eVecebdeedeebb`beccbdac_^_a\_`cccee`a_ccWd`XSSYP\V^ZQda]____[aEEE###############################
@HELIUM_000100422_612GNAAXX:7:1:8681:17597#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(C:34)":102},"score":4744,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagacgagagccattgagtctctgcacctatcctttatttattctcgctttgtgaaatccttgtttgttttcataaaacgggatttggctcaggattgcccagcgtgtctgg
+
CCCCCEEEDa^O^Wbc]]]^[YYcKc__ca_c[d`ceebed`ececfbbeeeeedab\c`]cdbb_eeeedddZ^dcccEEEEEEEEEEEEEEEEEEEEEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:10026#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":103,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(G:34)":86,"(C:02)->(A:34)":99,"(T:02)->(G:34)":102},"score":4267,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
ccagcagcgtagggcaatcctgagccaaatccgtgttttgagaaaacaaggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtacgctgctgg
+
CCCCC[UaU__abLXJZSUcZ_cX`_c`c_deeee_ceecd__eedecadcbe]eeecbeaa[cec`ebcEDEEEEEEEELEEEELEEEEEEEEEEEELEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8665:18393#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":107,"pairing_fast_score":0.933,"pairing_mismatches":{"(A:02)->(G:31)":91,"(G:34)->(T:02)":4},"score":2604,"score_norm":0.981,"seq_a_single":1,"seq_ab_match":105,"seq_b_single":1}
ccagatcgcgaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttctcaaaacacggatttggctcaggattgccctcgcgatctgg
+
CEELEEEEEEDEECEEEEEEEEEEEEEEEEEEEEEEEEEd^c^`febce`c_ccb_eedade_eOb^ceb_cc`ccEEEEEEEEEEEEEEIEEEEEEEEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8681:1186#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(G:34)->(A:02)":10},"score":4824,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
gtcgactggggcaatcctgagccaaatccgtgttttgagaaaacaagaaggttctcgaactagaatccaaaggaaaaggataggtgcagagactcaatggtcgcgatctgg
+
CCCEEEEEELEEEEEEEEc__Xeecededeeeeeedebddecfeebdddedeeee[__`^UZZYS\\K^[_cY_`^[cNXRQQIR_[Z`aeZTbEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8628:13164#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":106,"pairing_fast_score":0.806,"pairing_mismatches":{"(A:34)->(T:10)":30,"(C:02)->(A:34)":94,"(C:19)->(T:34)":37,"(G:34)->(C:02)":15,"(T:07)->(C:29)":42},"score":1727,"score_norm":0.953,"seq_a_single":2,"seq_ab_match":101,"seq_b_single":2}
ctccagacatcgcgggcaatcctgagccaaatcctgttttccaaaaacaaacaaaggttcagaaggtgaaaaaaggataggtgcagagactcaatgggcgatgtctggag
+
##DDEDEEEDECAALEEEaZGYX_cc`b^FOVGKRXDQNQQB^[^dc`Z\aabEEBCE>ECEE<>=>1BAEEEE@EEDEEEBEEEEEEEEEEELEEEECEDEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8614:4719#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":81,"pairing_fast_score":0.897,"pairing_mismatches":{"(C:02)->(G:33)":97,"(G:02)->(C:32)":93},"score":1529,"score_norm":0.975,"seq_a_single":27,"seq_ab_match":79,"seq_b_single":27}
ccagagcagtcgatatccgttgccgagagtcatattttttttgttgccttggccttggcaaccgacatagcccttgagcgactcgccggtgcctcagcttttatcgtgttccttggcgtcatccgagcagtctgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEDFEEDDDDDDDDEBEEC,CBC:<2;;?A;AAAdd\]`aTaU]^\\^_bbbd[VZM[BAJDC4KDEEEEDDEEEECCCCCCCACBCCABCCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8680:15314#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5086,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
gtcgactggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagtcgactgg
+
CCCZRRZcbacc`]aRcccb_aceeee^eefecbfeced_ccdee`dfedccdee[[[[]_a`a`__Xa\\cZca`^cEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8613:10548#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":92,"pairing_fast_score":0.91,"pairing_mismatches":{"(A:02)->(C:34)":102,"(G:34)->(A:07)":29},"score":2727,"score_norm":0.978,"seq_a_single":16,"seq_ab_match":90,"seq_b_single":16}
ccagaaccgattcggttggggcgaccaaggaaaaatcatcctcaattataaagatatactaatcaatcaacagaccctaaatagatcacagaataagctaccttagggataacagacgtcacgg
+
CCCCCCCCCCCCCCCC^ddUQRRRSYQYGeceedc_dadZa`\^dUdcaaeedcebe][dEEBEEE4EEEEEECEEEEEEEEEEEEEEEEEEEEEEEEEEELEEEEEECCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:14928#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(C:23)->(G:02)":37},"score":985,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
aacacgctcttccgatcgccatgcagattagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctctgcatggagaccgcacgagagctcc
+
##################CCCCCCCCBC5<:@9+:=A:;B;<9@ZIPYX]PZHTPVWQ[aGXD>D4DDD@4-=D<D@AD7D?DDDDD>A<<<<<<A4<D@DDDDDDDD##################
@HELIUM_000100422_612GNAAXX:7:1:8703:10808#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":105},"score":4218,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccagcagatcggggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgatctgctgg
+
CCCCCCCCEEc`ca^dY]Z^Wc\cZ_]`Z\Rbcccca[aHZW_dbeddddbceeeeaeee\Zaa_XVY]Xc__ccEEEEEEEEEEEEEEEEFEEEEEEEEEEEELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8659:6354#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":86,"pairing_fast_score":0.952,"pairing_mismatches":{"(C:12)->(T:30)":78},"score":1989,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ccacgcactagccgaaggcgtcaaggaacactgtgcctaacccggggaagcggctggcttgctagctgcccctcgtgttgcaatgctatttaatccacacgactctcggcaacggatatcctatgcgtgg
+
CCCCCCCCCCCCCCCCCCCC6?AAAEEEEBEDBBECECE?BEBBBEBECA=7)<=A:^VYbXZ`]SZSNIGaUO_]XAQI\LKQXYPK[P\]ZO]D=D@DC>CDD>DDBBB>>BBB@=?BBBBBBBBBBB
@HELIUM_000100422_612GNAAXX:7:1:8648:3472#0/1 {"ali_dir":"right","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":4565,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
cgatctccatcgctgtgccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccttcttgttttctcaaaacacggatttggctcaggattgccagatcg
+
######:;ADAACCCDDDA<DDD_]`a_\XL\]ZcZca[ddadd\^dddc\^`aaaXW]aab`cZad^a]cadd^dd__dZ^accc^c^VaEEEDEEEEEEEEEEEEE######
@HELIUM_000100422_612GNAAXX:7:1:8630:1890#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":87,"pairing_fast_score":0.905,"pairing_mismatches":{"(A:02)->(G:34)":102,"(C:02)->(A:34)":88},"score":3386,"score_norm":0.977,"seq_a_single":21,"seq_ab_match":85,"seq_b_single":21}
ccagagcagtcgggcaatcctgagccaaatccttttttgttttcagaaaaaacaaacaaaacaaaagggcttcagaaagcaaaaataaacataaaggataggtgcagagactcaatgggactgctctgg
+
CCCCCCCCCCCCBCCCCCCCCEEEacaaaTLOO\W[Wca\][`Ycbb`db\\_[J]_]^Z][cQ\\`Zde`ebae[[]aDDDDEEEELEEEEEEEEEEEEELEEEEEECCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8672:5066#0/1 {"ali_dir":"right","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":94,"pairing_fast_score":0.989,"pairing_mismatches":{"(T:02)->(G:34)":108},"score":5448,"score_norm":0.989,"seq_a_single":14,"seq_ab_match":93,"seq_b_single":14}
cgctcttccgatctcccagcacactccattgagtctctgcacctatctaccttttttagtcagagaatctctgacttggatttggctcaggattgcccagtgtgctggcgatcggaggagcg
+
###??+?+<9,;?<YaY^Y]]^HXZZYYdeZfe_cc__bccdd]c]]cYeebdeeeeedcedaedfdfeee_e`ccaecfeb^eaebcbSORRQWaaEEDEEEFEEEL##############
@HELIUM_000100422_612GNAAXX:7:1:9321:6000#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgccgg:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgccgg","obimultiplex_forward_tag":"gcgccgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1622,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttacaccctt
+
CCCCCCCCCCCCBCACC@CEEEEeeedeee^ed[K^a^__SVc``ccC<C:BCEEEECEEEDEEEFEEFEEDEEEELDEELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8641:15424#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":4091,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgagcctcttccgatctccgcgcattggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgaatgcgcggagatcggaagagcggttcagcag
+
################?>58>B<^aXa]cYV_Wc]ce_eeebeecece_ccc[`a`baZbdddbebc]ee_cacd`dcccdLbEEEDDEEEEEDDEEEBEEEEEECCE#######################
@HELIUM_000100422_612GNAAXX:7:1:8632:11896#0/1 {"ali_dir":"left","ali_length":80,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":80,"pairing_fast_score":0.74,"pairing_mismatches":{"(A:02)->(G:34)":106,"(A:33)->(T:02)":37,"(A:34)->(T:02)":31,"(C:02)->(A:35)":107,"(C:34)->(A:02)":34,"(G:31)->(A:02)":38,"(G:34)->(C:02)":43,"(G:34)->(T:02)":46,"(T:34)->(A:02)":30},"score":1756,"score_norm":0.875,"seq_a_single":28,"seq_ab_match":70,"seq_b_single":28}
agtgcatcggcacggcatgtgccaaggaatactcttagtgacgatgtgtctcgtgcccattcacggcgtgcgggatgcattgaccaaccacgaatatacaataacgactctcggcaacggatatccgatgcactgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCLLLELECKIBEEBLEDLCE=EEBEDEEDDCCCeaZ^bbbabeM\bWQb]]YMDMJBM_bc__\aEEEEFEEEEEEELMECCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8684:4696#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(A:34)->(C:02)":27},"score":3741,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
cccgacgctacgggcaatcctgagccaactccttttgtcaaaagcaaaaaatactcaaaagaaaaaaataataaaaaagcaagaaaaaaaaaggataggtgcagagactcaatgggtagcgtcggg
+
CCCCCCCCCCCCCCCCCCEEEEEEEELEEEDbcaaaaNZaa\XaUQ_Z\[\cYOe\cab]ddc^cdb_ac[dcbe___]YQQQPLb[]S]cEEEEEEEEEDEEEEEDECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8805:21248#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3872,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
tataccgccgccacccatatctacactcnnnnnnnnnncgacgctcttccgatctccacggtactagaacaggctcctctagcatagtggggtatctaagtaccgtggagatcggaagagcggttcagcaggaatgccgagacagatctcgtatgcggtcttc
+
######################################A+AAAAAA<@AC@CCB:dcdeeeeeeedeeceeeeeeeeeeeeeadeeebebcd[dcaeeeeceedeeee:C;=8B?C?CC@CC@CC######################################
@HELIUM_000100422_612GNAAXX:7:1:8681:3041#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":102,"pairing_fast_score":0.636,"pairing_mismatches":{"(A:02)->(C:30)":103,"(A:02)->(C:31)":108,"(A:02)->(C:33)":106,"(A:02)->(G:33)":96,"(A:20)->(C:02)":28,"(C:02)->(G:33)":100,"(C:02)->(T:31)":105,"(C:08)->(A:20)":57,"(C:19)->(G:02)":12,"(G:02)->(T:33)":88,"(T:24)->(G:02)":15,"(T:33)->(G:09)":36},"score":2627,"score_norm":0.882,"seq_a_single":6,"seq_ab_match":90,"seq_b_single":6}
ccgcgcgcgatccattgagtctctgcaactatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccatcgcgcgtgg
+
BB0B<?9A9>/=;;BDABDDDDDCDDB>_aH[[NMELQMI]T[_R[_TP`VRTZ\K8ZZQ^PYZ]SVZV^`YZR^cVcccW_@@DCDKDDDDDDBKDCDK>>H>IKDI@=BBBB
@HELIUM_000100422_612GNAAXX:7:1:8664:20563#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":73,"pairing_fast_score":0.786,"pairing_mismatches":{"(A:34)->(G:09)":66,"(A:34)->(T:02)":42,"(C:34)->(G:02)":51,"(G:34)->(T:02)":38},"score":1769,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacactgtcggatatccgttgccgagagtcgtttatggttaatgagaggccacgacccgtgtgcacaccgaaaacgggacaacacgtgacgcacccttcctatgttttgttttccttggcacatgccgtgcctgtgctgtgg
+
CCCCCCCCCCCCCCCCCCDCCCCCCCCCCC?7???EELEEELAEFEEEE=LEEaaS^\caddb`cF``cQ[b_acUeI`ab`DEBE?E:DEEEDEEEEEEEBEEDDDDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCDCCCC
@HELIUM_000100422_612GNAAXX:7:1:8651:8802#0/1 {"ali_dir":"right","ali_length":95,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":95,"pairing_fast_score":0.957,"pairing_mismatches":{"(C:34)->(G:02)":36},"score":3106,"score_norm":0.989,"seq_a_single":13,"seq_ab_match":94,"seq_b_single":13}
tctcttccggtctcgacgaccattgagtctctgcacctatcccgctttctaaattctggtttgttcgcgtaacccaggatttggctcaggattgccctcgtcgtctggagatcgcaggagc
+
#############EEEEEEEEEEEAEEEEEEEEDELEEa\YX^bUeeebeeeceeecXddeeebefe^b_]ac`cdc^^\^^aEEEEEEEEEEEEEEECEEEEEEEEE#############
@HELIUM_000100422_612GNAAXX:7:1:9321:3704#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:02)->(A:34)":68,"(T:34)->(A:18)":21},"score":1724,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCC?CCCCCCCCCCBBC?CcD_ceeedTeebecbTa_bU]Y^^a__aEEEEAEEEDEEEEBEEELEELEEEEEEEELEDELCCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8661:4464#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":100,"pairing_fast_score":0.979,"pairing_mismatches":{"(T:02)->(G:34)":107},"score":5878,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tccgatctcccactgtacgccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtaacccaggatttggctcaggattgccccgtacagtgggagatcgca
+
########EEaZ\cedceecedceZebeaaa^Necceceeee_eeeeeeaeeeeefedeeeeefeeedecceceeabb^^bb^[Xa\YRJRRR]]\aEEEEEEEEELE########
@HELIUM_000100422_612GNAAXX:7:1:8612:7059#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":106,"pairing_fast_score":0.99,"pairing_mismatches":{"(T:02)->(G:34)":108},"score":4895,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccacagtcgcagggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggtgcgtctgtggag
+
##EEa^aWX\_a_\c_aZZYUYVed_eeccc_a\\bT\]eaddddeddcdedde_debeeee`c`aZ^]`aaeed]eEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:8654:11302#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":100,"pairing_fast_score":0.918,"pairing_mismatches":{"(C:02)->(A:34)":83,"(T:02)->(G:34)":105},"score":3817,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
ccacagcagctgggcaatcctgagccaaatccttctttccgaaaacaaacaaataaaagttcagaaactgaaaatcaaaaaaagataggtgcagagactcaatggagctgctgtgg
+
CCCCCCCCEEEEEEEEEEBEEeU]ee_aaaaaSaa_eddedddeeddddcedddffdce_ebcecfea_b`ddccDDDDEEELEEEEEEEEEEEEEEEEEEEEELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8701:9524#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":106,"pairing_fast_score":0.99,"pairing_mismatches":{"(C:02)->(G:34)":108},"score":5627,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccagagtacgcccattgagtctctgcacctatccctttttctttcggaatctttatttgtttgccaaaaaaaaaggatttggctcaggattgcccgcgtactctggag
+
3?^^^^aZTYc][daLbedeaece_bbcedebceaebVbbe`ebb`b`bded`be_abe_ZaSHR[Zb`bceebbb_W]abac\cdcEEEEEEEEEEEDEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:9091:12436#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3904,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
tatacggcgaccaccgagatctacactctttccctacacgacgctcttccgatctccacggtacttagataccccactatgctagaggagcctgttctagtaccgtggagatcggaagagcggttcagcaggaatgccgagaccgataccgtatgcggtcttc
+
####A5AA0<:A/AA<A@=AA8A/.///<7<<<8+59-AC=CCCCCCCCCCCCCCeedeeeeeeeeeedebeeeeeeeeeeeeeeeeeeedeeeeeefdebedeecebCCA@?CAC@CCCCB;C@886880(0+067/79AA>:=,,+)'000,/@'99@?:?
@HELIUM_000100422_612GNAAXX:7:1:8632:17795#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":103,"pairing_fast_score":0.99,"pairing_mismatches":{"(G:34)->(T:02)":6},"score":3639,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagtgcacacccattgagtctctgcacctatccttttcctttggattctagttcgagaaccccttgttttctcaaaacacggatttggctcaggattgcccgtgtgcacagg
+
CCCCCLEEEEEEEDEEEEEbdbd]Zacc^\^^R^[\\]\^U]\L\^Xa^LVZacc^eedeededdeebee`dcc`EEEEEEEEEEEEEEEEEEEFEEEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:8922#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":89,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(C:34)":106,"(A:02)->(T:34)":108,"(C:02)->(A:34)":95,"(C:34)->(G:02)":32,"(T:34)->(A:02)":20,"(T:34)->(A:20)":38},"score":2290,"score_norm":0.933,"seq_a_single":19,"seq_ab_match":83,"seq_b_single":19}
ccaacacactacaaccaaatccaatttcacgccaatattagaaccaacaatccaaataaacaaatatcaatgtaatccatcattccacttagaaacaagctgcaccttgacctgaaatgtgtgttgg
+
CC3CCCCCCCCCCBCCCCBLEEEEEEEEEEELd\dadDaa]_ZUZXW__[Z]`Xee\\eb]_e``b_T]b?EECEAEE@CECBEEEEEDEEEEDLEEEEEEEECELELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8640:19797#0/1 {"ali_dir":"right","ali_length":78,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":75,"pairing_fast_overlap":78,"pairing_fast_score":1,"score":4147,"score_norm":1,"seq_a_single":30,"seq_ab_match":78,"seq_b_single":30}
actctttccctacacgacgctttccgatctcctcggtcgccccaaccgaatctttaaaaatttttattttatcagattaagctctatagggtcttcttggaggcacggagatcggaagagcggttcagcaggaatgcg
+
#################?@@C@C;CCC?CAaedabc]^ePeddcceccdeeeeeeeeeedccdceebbb`bdeceZ_aa]`]bccbeUcEEEEEEEEEEEEEEEEDEE##############################
@HELIUM_000100422_612GNAAXX:7:1:9134:20690#0/1 {"ali_dir":"right","ali_length":52,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":49,"pairing_fast_overlap":52,"pairing_fast_score":1,"score":3789,"score_norm":1,"seq_a_single":56,"seq_ab_match":52,"seq_b_single":56}
atatacagcgacccccgagatctacactctttccctacacgacgctcttccgatctcacggtacttagataccccactatgctagaggagcctgttctagtaccgtggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgccgtcttct
+
###############?(7==?A=6<::::)+.:A==?B@@?CC@CCCCCCCCCCCCeeeeeeeeeeeeebdeeeeeddedaedfeeeaecdeec`de`ecaeecebe_CC;=6>=?AAB?BCCCAA7:97<CC?3@############################
@HELIUM_000100422_612GNAAXX:7:1:8641:2318#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":106,"pairing_fast_score":0.913,"pairing_mismatches":{"(A:02)->(C:34)":104,"(A:02)->(G:34)":90,"(C:02)->(T:34)":103},"score":5066,"score_norm":0.972,"seq_a_single":2,"seq_ab_match":103,"seq_b_single":2}
ctccacgatgcagccattgagtctctgcacctatccctttttttttcgttttctgaatctttgttttaagaaaacaggatttggctcaggattgcccctgcatcgtggag
+
##EEEEEa[]YbQYaTU^[]]][ORRLQVKc_`_`aaZ_deeedde^ededee]ed_edddceebdYcZeeeeZZaebXcbWeabedEELEEEEEEEEEEEELLEEEE##
@HELIUM_000100422_612GNAAXX:7:1:9232:7986#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tatctca:tatctca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatctca","obimultiplex_forward_tag":"tatctca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatctca","obimultiplex_reverse_tag":"tatctca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4337,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
ggcct
+
eeade
@HELIUM_000100422_612GNAAXX:7:1:8628:19182#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":106,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:34)->(A:02)":14},"score":4727,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccagacacgctccattgagtctctgcacctatccttttttcaccttctaggcctttgtttgtttttggaaaataggatttggctcaggattgcccctacgcagtggag
+
##EEEEEEEEEEELEFEEEb_caaa__c[[\\]eTe_ceeeecaccbdda[eecbdeedddcdfeYdddbeeedee]``Y_aeb]eaEEEEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8694:6133#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":4629,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ttccctcacgacgctttccgatctccagacgacgagggcaatcctgagccaaatcctgttttccgaaaacaaaggataggtgcagagactcaatggtcgtcgtctggaagatcggaagagcgctccagcgga
+
####?7<=<=2:7A48:7<)@=@Beb\e][PRS^`adbddbdfeeeccdedff`de`ebeeceeb_cfaefbbdc]eRebaa`bceeb_bcEEECEEEEEEEEEEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8609:2044#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":43,"pairing_fast_overlap":86,"pairing_fast_score":0.518,"pairing_mismatches":{"(A:02)->(C:34)":102,"(A:31)->(G:02)":61,"(A:32)->(G:02)":40,"(A:34)->(C:02)":45,"(A:34)->(G:02)":52,"(C:02)->(A:33)":92,"(C:02)->(G:34)":108,"(C:02)->(T:34)":103,"(C:34)->(G:02)":59,"(G:34)->(A:02)":43,"(T:33)->(A:02)":39},"score":1314,"score_norm":0.826,"seq_a_single":22,"seq_ab_match":71,"seq_b_single":22}
ccacgagcgtcggatgacgccaaggaacacggtaaagctaaggcaccggcgagcctttcgagggctccgtcggctgccgaggccaacagaaaaaaatatgactctcggcaacggatatccactgcagtgg
+
CCCCCCCCCCCCCBCCCCCCCCEELLEEEEELFEEEEDKJEELDLELEBDELEEDE:ELEIED@E?dURNYc^d]e^dddbVYaWa`U]`DKEEEDEEEEBLLEEEELCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9225:6309#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtgcctc:gtgcctc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtgcctc","obimultiplex_forward_tag":"gtgcctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgcctc","obimultiplex_reverse_tag":"gtgcctc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":31,"pairing_fast_overlap":62,"pairing_fast_score":0.525,"pairing_mismatches":{"(C:34)->(A:02)":30,"(T:23)->(G:02)":51,"(T:26)->(G:02)":36,"(T:32)->(G:02)":39,"(T:33)->(A:02)":21},"score":383,"score_norm":0.855,"seq_a_single":46,"seq_ab_match":53,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCDCCCCCCCCKEEEEELDELEEED@DCCJB?C>EECCEECAC?UXZZS\`bEBEEEEEDEBEELEELDFLECCBCCC=CCCCCC@CCCC@
@HELIUM_000100422_612GNAAXX:7:1:9379:18493#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ggttatt:tgtattc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggttatt","obimultiplex_forward_tag":"ggttatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtattc","obimultiplex_reverse_tag":"tgtattc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(C:32)->(A:02)":27,"(C:34)->(A:02)":30,"(G:34)->(A:02)":20,"(T:26)->(G:02)":51,"(T:33)->(A:02)":21,"(T:33)->(C:02)":26},"score":341,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCACCBCCCCCCBLKEDEEKJDELEEEDB?DDDDCEEECEEEEAD=BOPKNRb`c?DEEEEEEEEEEEEEEFEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8655:9158#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":102,"pairing_fast_score":0.879,"pairing_mismatches":{"(A:02)->(C:34)":102,"(C:02)->(G:34)":96,"(T:34)->(G:20)":15},"score":5737,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagagtacgcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgcgtactctgg
+
CCCCCCEEEa]U]bDU\\_cc`c\XabY\ceecec`^caeecebebbeebebeedefafeeaedadZeeeeddebaddddab`^Zac`aM_cc\aLEEEEELEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8647:9779#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":89,"pairing_fast_score":0.907,"pairing_mismatches":{"(A:02)->(C:34)":105,"(C:02)->(T:34)":107,"(G:34)->(A:12)":29},"score":2946,"score_norm":0.966,"seq_a_single":19,"seq_ab_match":86,"seq_b_single":19}
ccgcgaacgattcggttggggcgaccaaggaataaatcatcctacaaaactacgacacatatgtcactccatagaccctaaactaagatcatcaacaaagctaccttagggataacagcgttcgcgg
+
CCCCCCCCCCCCCCCCCCCcac_cQ]PZEXSZQPRRJUNZX]\[a\c\]\G[cc]cdcc]^U]]\YZYYW__bEEEEEFEEEEEEEEBDEEEEEEEEEEEEEEELFLECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8657:2169#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":89,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:34)->(T:02)":21,"(C:02)->(T:34)":97,"(G:02)->(A:34)":85,"(T:02)->(C:34)":99,"(T:02)->(G:34)":103},"score":2158,"score_norm":0.933,"seq_a_single":19,"seq_ab_match":83,"seq_b_single":19}
ccgaatatcctgttatccctaaggtagcttatctttaggtctattattagggtctttataaagatcaatgggtcttatggtgtaaggatgatttattccttggtcgccccaaccgaatgatattcgg
+
CCDCCCCCCCCCCCCCCCCELEEEEEFEEaR\^R[[^WZ^U_YMZSZcbZc^Tedaeeeedee\aCDEEEEEEEEEEEELEEECLEEEEDEEEEEELELEEELEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8788:8523#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:13)->(a:31)":59},"score":3247,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctg
+
CCCCCCCCCCCCCCCCCACdZcaebe[Kccdb\`IYc`bb]^ecbbbaW\aXcad^`XB[RYVa_`[caecEEEEEECEEECCCCCCCACCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8705:5681#0/1 {"ali_dir":"right","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":73,"pairing_fast_score":1,"score":5171,"score_norm":1,"seq_a_single":35,"seq_ab_match":73,"seq_b_single":35}
ctacactcttgccctacacgacgctcttccgatctccgacggagccctcgaaaggctcgccggtgccttagctttaccgtgttccttggcgtcatcccagagctctggagatcggaagagcggttcagcaggaatgccgagac
+
##################@==@CAAA;4==*68B<_dcbedecdbde^cecaeeegeeecebcbeeeedeceeeeedeeeeeeebeceXbcc_]M_cc__eeacec_\AAC9@A@C8CC?6807*?;8>9<.?##########
@HELIUM_000100422_612GNAAXX:7:1:8641:18826#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acagctc:taccgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acagctc","obimultiplex_forward_tag":"acagctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taccgtg","obimultiplex_reverse_tag":"taccgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(a:25)->(c:32)":69,"(c:21)->(t:27)":38},"score":2055,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagagaactactagcaacagattaaacctcaaaggacttggcagtgctttatacccct
+
CAC7<?<?CCCCCACCBCLELEEDEEBCECb]WZWZ[=S\WLXT[WMIHRLNYXMO\W\c`bQad]^cAV\cEEEEEEAEE>AA=AACCCCCCACCCC?
@HELIUM_000100422_612GNAAXX:7:1:8612:8686#0/1 {"ali_dir":"left","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":60,"pairing_fast_overlap":93,"pairing_fast_score":0.667,"pairing_mismatches":{"(C:02)->(A:17)":81,"(C:02)->(A:31)":108,"(C:02)->(G:33)":99,"(C:02)->(T:30)":70,"(C:27)->(G:02)":28,"(C:32)->(G:02)":18,"(G:24)->(T:02)":31,"(T:02)->(C:28)":95,"(T:21)->(G:02)":25},"score":309,"score_norm":0.903,"seq_a_single":15,"seq_ab_match":84,"seq_b_single":15}
cctggtaagctgttatccctaaggtagcttggtctttaatcttgaaatgttcgtttatagacttataggtcttttgtttaaaggatgatttatcctaggtcgccccaaccgaatcttaccagg
+
8:A@@=AAA?262:>CCJAC@)@:?<<E>3B<:C@CCC_R__[]3?DBDDD8CDDBDD8D8DDDDDDDDH???DDDBDDD;DDDDDDDDDC@DCFDDDKDDDDDDD?IBBB@BBBBB@@AA@=
@HELIUM_000100422_612GNAAXX:7:55:7513:15393#0/1_sub[28..127] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:cctcctt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":2,"obimultiplex_reverse_match":"aggaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"cctcctt","obimultiplex_reverse_tag":"cctcctt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":61,"pairing_fast_score":0.845,"pairing_mismatches":{"(T:34)->(A:02)":21},"score":2407,"score_norm":0.934,"seq_a_single":47,"seq_ab_match":57,"seq_b_single":47}
ttagccctaaacacaagtaattaatataacaaaattgttcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCCBCCCCCC@CCLedebf\_c^]]e^a_baeeRbdcbec]WGWVW^\T][bEECEEEEEEDEAEELEELFEELCCCCCCCCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:18011#0/1 {"ali_dir":"right","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":92,"pairing_fast_score":1,"score":4603,"score_norm":1,"seq_a_single":16,"seq_ab_match":92,"seq_b_single":16}
cacgctcttccgatctccaacggaacaagaagaccctatagagctttattcactagcttatacaaactattactcttaatgaattcggttggggcgaccttccgttggagatcggaggagcggt
+
###?=?=>?*?;6=A:TceedReeeceeMYcb^b]beeebacaaeeeeeeeeeeec^eeeedededecedeceeeeeedbbEEEEEEEEEEEEEEEEEEEEEEEEEEE################
@HELIUM_000100422_612GNAAXX:7:1:9310:4368#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gaggctt:gaggctt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaggctt","obimultiplex_forward_tag":"gaggctt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaggctt","obimultiplex_reverse_tag":"gaggctt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:02)->(G:10)":57,"(A:31)->(G:07)":48},"score":1332,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcgatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCC@CCCCCCCCCCAC;CDBEEEbf[XaXZc\_PWSN.Q_ZZYc_aID*-*<>9=849::6BECEEB@B5BC?EEI7DBDBCBCCB?BCCCCCCCCAC@
@HELIUM_000100422_612GNAAXX:7:1:8685:9785#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4776,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacgactagcgggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggcgtacgtctgg
+
CCDEEEEEEEEEEEEEEEDEFd^aTda___`ZbbeeedcededefdXded`eddecebaddeeccbbeeec_]ccdcdSdZYVYY^\YY^cEEEEEEEEEEEEEEEEDCCC
@HELIUM_000100422_612GNAAXX:7:1:8631:3947#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":86,"pairing_fast_score":0.952,"pairing_mismatches":{"(A:02)->(C:34)":99},"score":3933,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ctacacgacgctcctccgatctccaaccgcccaagaagaccctatagagcttaatttaacctactaaataagtaaaaattaaattcggttggggcgaccggcggttggggatcggcagagtggttcagaa
+
####################??a[RZSZ[TZZLQIIQRReWeccb_b`Zc`b`__ccXb`^d\ee`cedded]aa_Z]`a`[ceeeEEDBDDBEEEEELEEEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8656:13197#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":73,"pairing_fast_score":0.9,"pairing_mismatches":{"(A:02)->(C:34)":96,"(G:34)->(T:23)":38},"score":2853,"score_norm":0.973,"seq_a_single":35,"seq_ab_match":71,"seq_b_single":35}
ccacgactagcgatatccgttgccgagagtcgtttatggttaatgagaggccacgacccgtgtgcacaccgaaaacgggacaacacgtgacgcacccttcttaagttttgttttccttggcacatgccgtgcctctctcgtgg
+
CCCCCCCCCCCCCCCCCCDCCCCCCCCCCBCBCCCYUC`YaO`JX_eeS^\^`aJ\_``adedc]bbbOVDJZ`c_\YRb^a[aaEEEDCEEEDELEEDEEEEEDDDDCCCCDCCCDCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8672:8036#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5749,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccacgtgtcgcccattgagtctctgcacctatcctttttcaccttctggggcctttgtttgtttttggaaaataggatttggctcaggattgcccgctgtctctggag
+
##EEEEEEEdcd\]abeeaedee_da_a`[aY\\_a_`Uc_cZdeedeeeeeddaddedddadeeddbd]edeeceede`eceLbaeQRPRRabEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8679:12983#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":4761,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
ccagacatcgcccattgagtctctgcacctatcctttttttattcttgctttctgaaccctcttttgttttctgaaataaggatttggctcaggattgcccgcgatgtctgg
+
CCCCEEEEaaSa^NZ[Ucc_c_a]U`bY\[a`bdS\Vd^bddcddeebTeded^ddbd`eedbbd]eeddddedfc^ccEEEEDEEEEEEEEEEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8642:8561#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":102,"pairing_fast_score":0.939,"pairing_mismatches":{"(C:02)->(T:22)":106,"(C:02)->(T:29)":104,"(T:02)->(G:32)":103},"score":5095,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccacgatcgcggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtgtgctcgtgg
+
BBBBA>SMWKce\`_[__ZY_cY`bbcccc_Wc`c__c]ac^^cccca\^_ccc`accc^bcZW^[`_c]_[_Y__bceccIcbDDDDDDDDDDDDDDDAACJG5@9:6:??;?
@HELIUM_000100422_612GNAAXX:7:1:8664:17047#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":3775,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctacactctttccctacacgacgctcttccgatctccacggtacccgtatatacatacgtatacgtctgaatgaaagagttggtgcgtcatcggagatcggaagagcggttaagcaggaatgccgagaccgatatcgtatgcg
+
########################A:AA61;@?90<6:A=CC<C;CCCCeefe\_c_ceededbeebeeeedbeeeedeeeeefU_a`]cc^cc^dacdcbeEEEEEE#################################################
@HELIUM_000100422_612GNAAXX:7:1:8788:18886#0/1_sub[74..81] {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgagt:ttcgagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgagt","obimultiplex_forward_tag":"ttcgagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgagt","obimultiplex_reverse_tag":"ttcgagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4116,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ccagagca
+
NZYOJeed
@HELIUM_000100422_612GNAAXX:7:1:8662:10718#0/1 {"ali_dir":"left","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":74,"pairing_fast_score":0.944,"pairing_mismatches":{"(A:34)->(G:02)":44},"score":3339,"score_norm":0.986,"seq_a_single":34,"seq_ab_match":73,"seq_b_single":34}
ccatcacgtcggcacggctgtgccaaggaaaacaaaactcaagaagggtgcgtctcgtgttgccccgttttcggtgtgcatgcgggtcgcggcctctcattaaccataaacgactctcggcaacggatatccgacgtgatgg
+
CCCCCCCCCCCCCDCCBCCCCCCCCCCBCCCCCCDDDDE@CEELEEEEBEEAb`e__a^b_WVZT^W]ce_]Rbb_]]`YNLT[WKV[WW`K^^V^^\Tc\`_`_YZYCCCCCCC@ACCCCCCCCBCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:5643#0/1 {"ali_dir":"right","ali_length":77,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":77,"pairing_fast_score":1,"score":5499,"score_norm":1,"seq_a_single":31,"seq_ab_match":77,"seq_b_single":31}
actctttccctacacgacgctcttccgatcttagcggggcaatcctgagccaagtccaatcgaagttgtcgatgggataggtgcagagactcaatggcgctactctggagatcggaagagcggttcagcaggaatgccg
+
##?CCACC>B9?A??00=?CCCCCCCC@CCCddeeceeedeceede_eeUeeedeeebdeee`ddceeeedbdeeeccecb_ee^ede_ce\abU^deeedb_ebdedBC#############################
@HELIUM_000100422_612GNAAXX:7:1:8682:5306#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":73,"pairing_fast_score":0.929,"pairing_mismatches":{"(G:34)->(T:02)":36,"(T:34)->(A:02)":40},"score":682,"score_norm":0.973,"seq_a_single":35,"seq_ab_match":71,"seq_b_single":35}
ccacagctgacgatatccgttgccgagagtcgtttgtaattacaaagagtccacgaaccgcatgcacaccgcgaacggggctacacggcacgagtccttctcatttatggttatccttggcacatgccgtgccagagtcgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCLEEDLEEEEEEEEFDE7EE@>A>5A11222EEEE_\`^\b[`\cEAECDEEEEEEEEAFE?EECCCCC1112*(///0CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8667:20372#0/1 {"ali_dir":"left","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":69,"pairing_fast_overlap":82,"pairing_fast_score":0.873,"pairing_mismatches":{"(C:31)->(G:02)":41,"(G:34)->(T:02)":29,"(T:02)->(G:34)":106},"score":1065,"score_norm":0.951,"seq_a_single":26,"seq_ab_match":78,"seq_b_single":26}
ccagagctctggatatccgttgccgagagtcatattttttctgttgccttagccttggcaaccgacatagcccttgagcggctcgccggtgcctcagcttttatcgtgttccttggcgtcatcccaagctctgg
+
CCCCCC=CBAACCCCCCCBCCCCCCBLELEEEEEEEEEBBI@BEECCY_[KGJAFVDBPP`^]I_[H_aC??A=EE=BEC?CAACDBEEBEBEEEEFEEEEEEEDLEECCBCCCCCBACCCCCCCBCCBCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8673:2835#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(A:02)->(C:34)":105,"(C:02)->(T:34)":107},"score":3068,"score_norm":0.978,"seq_a_single":18,"seq_ab_match":88,"seq_b_single":18}
ccaattaactacaaccaaatccaatttcatgccaatataaaaatcaacaatccaataaaacaaataacaatgtaatccatcattcactcagaaacaagctgcaccttgacctgaaatgttaattgg
+
CCCCCCCCCCCCCCCCCCEFEa\a_ac_aZNeeee`dbdeeedab_c`_cb`daedddeddadddEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEELELECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8675:11508#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":106,"pairing_fast_score":0.99,"pairing_mismatches":{"(T:02)->(G:34)":108},"score":5916,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccagagacagcgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatgggctgtctctggag
+
CCeedeecde`beee]eefddbdcec_`ddaZd`cdccdedddddeedcddddedddeeeddccc\\_`aaXeeebcRQQKRZYVXZcbEEEEEEEEEEEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:8654:5417#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":103,"pairing_fast_score":1,"score":4382,"score_norm":1,"seq_a_single":5,"seq_ab_match":103,"seq_b_single":5}
ccacgtcgagtgggcaatcctgagccaaatcacgttttccgaaaacaaaaaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggactcgacacgtgg
+
CCCCCEEEEEEEEEc_c_W[XX\^O\]PZSYZZ_Y[]bTdbZdbddddd`ddb``]_[`ceeeba_accbdbddddbdbdaEEEEEEEEEEDEEEEEFEBEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:9241:3295#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttaggat:ttaggat)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttaggat","obimultiplex_forward_tag":"ttaggat","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttaggat","obimultiplex_reverse_tag":"ttaggat","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(G:34)->(T:02)":20,"(T:32)->(A:02)":21},"score":2032,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCBBCCCCCBBCCBLJE`]`XM[ZV\bYMRNE\U]GaKWWWU[UV\P\QYXY[c_bEEEEEEEEEDEEEEELBEEECCCCCCCCCCCCCCCCACC
@HELIUM_000100422_612GNAAXX:7:1:8670:15432#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5560,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccactgcacgtccattgagtctctgcacctatccttttttattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgcccacgtgcagtgg
+
CCCY\U^^Ua^]\``\Ze^cec]_Ncccc_cbe`a`_ddeeddddeecdeeeeba]`M_aa`^aXdeddd[^_cceec]a`Y_baaEFEDEEEEEEEDEEEFEEEEEEBCC
@HELIUM_000100422_612GNAAXX:7:1:8823:4136#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:32)->(A:02)":27},"score":133,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCBCCCBCCCCCCCBCCBDCEEEEEJBCCCDDDDBBA9>5>>>9>>ZS927DCEEBEEEEBDEEEEEDEBEEEDELEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:9874#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":73,"pairing_fast_score":0.886,"pairing_mismatches":{"(A:34)->(G:06)":66,"(C:02)->(T:34)":99},"score":2241,"score_norm":0.973,"seq_a_single":35,"seq_ab_match":71,"seq_b_single":35}
ccatgcagtgcgatatccgttgccgagagtcgtttatggttattgagaggccacgacccatgcacaccccgcgaacgaggcatcacgagacacgaccttcctatgttttgttttccttggcacatgccgtgcgcactgcatgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCC@ACACCCEDEEEEEEEeeeaK][\`c\`cabRO]]_aGbae_de`deb^d_EE?EECCEEEEEEEADEEELDEEEDD=DDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8949:11120#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gatctac:gatctac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatctac","obimultiplex_forward_tag":"gatctac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatctac","obimultiplex_reverse_tag":"gatctac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(T:02)->(A:34)":59},"score":1128,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
?AC4<;39CCCCCBCCCCCe\Udede__ed``ZUBF]L+<:)9:0:;-/?A+ADC8DELBEDEEEEEE>CEEEEEELDE@ECACC>CCCC@CCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8670:5128#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":86,"pairing_fast_score":0.964,"pairing_mismatches":{"(T:02)->(C:34)":106},"score":4166,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
cagctcgtctgatatccgttgccgagagtcgtgtggattaaatacgattgcaacacaagagatgaccagcaagctagccatgcccttgggttagacacagtgttccttgacgccttcggagacgagctgg
+
CCCCCCCCCCCCCDCCCCCCCC]aeeebbc`cTca`ca`eedebc]eada]bcbeefcbeeedZeb\^TZRRRJRRRRPQSRRRQcZcZcEEEEEEEEEEEEEEELEECCCCCC8CCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8730:15634#0/1_sub[82..88] {"ali_dir":"right","ali_length":54,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:gatctgc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatctgc","obimultiplex_reverse_tag":"gatctgc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":54,"pairing_fast_score":1,"score":3913,"score_norm":1,"seq_a_single":54,"seq_ab_match":54,"seq_b_single":54}
caactag
+
eeeeeee
@HELIUM_000100422_612GNAAXX:7:1:8658:9947#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":5053,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
tccgatctcccactgactcgggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatgggagtcagtgggagatcgga
+
A=B??A=:W^ab]Uddcdeceeeeeededaae`dccdccc`ccdeeddeeefeeeeeeedeeedeebeeeebbeecdeEEEEEEEEEEEEEEEEEEEEEEEEEEDEEE########
@HELIUM_000100422_612GNAAXX:7:1:8669:14414#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":100,"pairing_fast_score":0.845,"pairing_mismatches":{"(A:34)->(G:23)":36,"(A:34)->(T:10)":35,"(C:02)->(G:33)":107,"(C:02)->(T:30)":92,"(G:31)->(T:10)":43},"score":3901,"score_norm":0.95,"seq_a_single":8,"seq_ab_match":95,"seq_b_single":8}
tccgatctcccactgtgacgggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagacttaatgggtcacagtgggagatcgga
+
########EEEEEEEEEEac_Sccdb`ca]LTVUFCQPWYX`C^]]\Cd\ZZdcOadddU^X^dddbddddcb_ZWT\[]R^^Y[``DA>CHA?DDDDDD>D?DDDKD########
@HELIUM_000100422_612GNAAXX:7:1:8620:11088#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5017,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctagcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggctgctagtggag
+
AAbee_ecaZa_TeUeedeefeebedeeeebdededdeeeedbdeeeeceeecccd_c_cc]ecbeebeaecEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEFEE##
@HELIUM_000100422_612GNAAXX:7:1:8646:13929#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":103,"pairing_fast_score":0.84,"pairing_mismatches":{"(A:27)->(T:02)":15,"(A:32)->(G:07)":43,"(C:02)->(G:34)":102,"(G:20)->(T:07)":39},"score":2202,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
cagacgacgagggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtcgtcgtctgg
+
AAAAACCCB:<7<8ECCCCCCCCCCCa]RQV_SZZ_[[9MFHE]]_O_[b^`^IXM\JW^a_`PabX``EEEECDEEEEECEEEEEEEEEEEEEEEDEEEELEDEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8654:10471#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgccgg:gatctac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgccgg","obimultiplex_forward_tag":"gcgccgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatctac","obimultiplex_reverse_tag":"gatctac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(g:15)->(t:34)":81},"score":3197,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCC?CCCCCCCCDCCCC;CCLEEDEEEEec``_`WacQZ_]W\]W^YOVXMWbac_^`cc_adUUa`^\bZUbcbcW_^^DCCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8692:15186#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":6040,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccatctctcttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccgagagatggagatcggaagagcgcttc
+
################BC_bbcceceeb[^XW]^c]ee\]^]Vee^deQ]\^\eeeeeeece_cc`^cdee_beedce]\]d_Z^]]a_cc_[\^Z\^\]]cbca_c`CAA###############
@HELIUM_000100422_612GNAAXX:7:1:8685:16234#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":101,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(G:34)":97,"(C:02)->(G:34)":91,"(C:02)->(T:34)":100},"score":4012,"score_norm":0.96,"seq_a_single":7,"seq_ab_match":97,"seq_b_single":7}
ccagcagcactccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttttttttctcaaaacacggatttggctcaggattgcccagtgctgctgg
+
CCCCCCCE:EEEEECEEEEEccc]]aK[a^d]_c_c_c_bcWccb]d[``cMccb]_^Vceebdb\`Ybebb``bW^Ya]aEEELEEEEELEEDEELEELEDEEEEEDBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:11277#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":87,"pairing_fast_score":1,"score":3863,"score_norm":1,"seq_a_single":21,"seq_ab_match":87,"seq_b_single":21}
ccgtgcctcccgtatatacatacgtatacgtcctgaaatactatctcaaaaaatgaatgacgacccgaatctttatttctttttttatatttatataaaaactgaatgaaagagttggtgcgttcgcgg
+
CCCCCCCCCCCCCCCCCCCCCEEDEEEEEEEEEDEbcdc[[`eaebdfeeeecacca`b`WU\Y^]____WXZTYbb\ZbdcdddYYPY[[aa`DEEEEEEEEEEEEECDCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8672:14560#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":59,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctgcaccacgctcatccgatctccgaagtagggtcgccccaaccgaatctaggaatatgatatcatatgggtagataaagctctatagggtcttcttgctacttcggagatcggaagagcggttcagcag
+
#######################CABABD:;D810221::95:<<<4<<.@94>=99>7@@C@;8>BB8<46>-<>;4C%%%%DC>7C1@5<=CC?<CBBB3CBBCCC#######################
@HELIUM_000100422_612GNAAXX:7:1:8703:8880#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":106,"pairing_fast_score":0.961,"pairing_mismatches":{"(A:02)->(G:34)":90},"score":5574,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccagatcgcgaccattgagtctctgcacctatcccttatttctttcggaatctttatttgtttgccaaaaagaaggatttggctcaggattgccctcgcgatctggag
+
78U___R_c`c__c`c\_]e_ce`c_cceMceebebeaeddeeeeecdeeeebddedddeddefbd_bb^ddeed_dbc_acEEEEEDELEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8954:4109#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgatga:gcgatga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgatga","obimultiplex_forward_tag":"gcgatga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgatga","obimultiplex_reverse_tag":"gcgatga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":1228,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCDCCCCCCCCCDCLBEEEEEEFEEFEEDDEDD=AC?:DC2C>C?EEBE=>5>=a]Z^_W_`a_____`[XV_EEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9172:12716#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgaacg:gcgaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgaacg","obimultiplex_forward_tag":"gcgaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgaacg","obimultiplex_reverse_tag":"gcgaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:30)->(T:10)":51},"score":2787,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCBBCCCCCCDCC\aca`a_Z\a__e_b[`]S][cS\_ab`KPFDBH[INL?NaZ\V^S]W\XaEEDEEELCE5EECBCCCBCCCCBCA<CCCC
@HELIUM_000100422_612GNAAXX:7:1:8699:9467#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":95},"score":5834,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagcgtcatgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctaggattgccccatgacgctgg
+
CCCCC_dcZZa]_dbccecc]c__acded_c_a]\]]Z]^abcea`e`ebb^e`edced`eee`addeccebbeeeaebeeeeXeed^eEEEEELEEEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8614:6314#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5107,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
cccatgtgtgcccattgagtctctgcacctatccttttttattataactttctgaatccttggttgttttcagaaaacaggatttggctcaggattgcccgcacacatggg
+
CCCEEEDEEEEEEEEEc__ccc`Za_b`a`cdad`_ddcee\ddeebeeebeddZeeecdee\_aebceaddceeeeeddZ_ac``I_cEEEEEEDEEEEEEDEEEEEDCC
@HELIUM_000100422_612GNAAXX:7:1:8661:7464#0/1 {"ali_dir":"left","ali_length":10,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":7,"pairing_fast_overlap":10,"pairing_fast_score":1,"score":-2,"score_norm":1,"seq_a_single":98,"seq_ab_match":10,"seq_b_single":98}
ccagagctctgccattgagtctctgcacccatcccttatttattctcgctttctgaaacctttgttttcctaaaacgggatttggctcaggatacccccagagctctgccattgagtctctgcacctatcccgtatttattctcgctttctgaaacctttgttttcataaaacgggatttggctcaggattgccccagagctctgg
+
>9929??;?4?<B??BA@BBB@B@ABABA(/(00/747;;A=AA:ABABA:3:965>AAA<B:AB?################################%%A@@<2@=@7;;9;=>68B8B@//00-=A:7:C=C?=????8CB@CBAA@AACCCBC>+/CCBCBCC@CCCC@CBCCCCCBCCCACCCCC8CCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8788:5624#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agtcgtc:agtcgtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtcgtc","obimultiplex_forward_tag":"agtcgtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtcgtc","obimultiplex_reverse_tag":"agtcgtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{},"score":1359,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCBCCCCCCCCCCCCCLEEEEEEEDCEEECDDLDDBEEC-B:>;88<?CC_^M^\VaQ\_`dcceb_cR^a?=?;EBEECCBCCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8669:4984#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:02)->(G:34)":92},"score":5462,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccagatcgacggggcaatcctgagccaaatccatgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggcatgtgc
+
CCCbT]_Y\^c^bJ_bXaXa\bdcXc[X[aU`Wdcdddcee[\_a^edddeeefaeeefbded_fbeeefedece_dddaea_eEEEEEEELEEFEEEEEEEEEEEEECAC
@HELIUM_000100422_612GNAAXX:7:1:9076:17411#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atgatca:atgatca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgatca","obimultiplex_forward_tag":"atgatca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atgatca","obimultiplex_reverse_tag":"atgatca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(T:26)->(A:10)":35},"score":1773,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCCCCCCCCCAC_Uce^ee_^ddc]`W>U`__[^^\Xb]^<><>7DAA-C?EE?EEEEEEEEEEEEEEEDDEEECACCCCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8723:19113#0/1_sub[28..133] {"ali_dir":"left","ali_length":56,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tggtggc:tggtggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggtggc","obimultiplex_forward_tag":"tggtggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtggc","obimultiplex_reverse_tag":"tggtggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":56,"pairing_fast_score":1,"score":2599,"score_norm":1,"seq_a_single":52,"seq_ab_match":56,"seq_b_single":52}
ttagccctaaacgtaaactgcaaactattctataacaaaataattcgccagagaactactagcaacagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCACCCCCCCCCBCDEEEEEEEEEEDEEDDE`U`_eabeced_[_]Y_`]bZceead[bcbaaH^^add]CCCDCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8675:7420#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":5776,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ctacgcgacgctcttccgatctccaaccgccggtcgccccaaccgaatttaatttttacttatttagtaggttaaattaagctctatagggtcttcttgggcggttggagatcggaagagcggttcagaa
+
######################EcdTddcc_Z_fdaee_ebceceeeeeeeeeeeeeaeeeeeeedf\cccdbcecece`aLcc__c_c`\Sca`c__cXc_\X\U^I@#####################
@HELIUM_000100422_612GNAAXX:7:1:8678:17826#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":81,"pairing_fast_score":0.872,"pairing_mismatches":{"(A:34)->(G:02)":29,"(G:30)->(A:17)":63,"(G:30)->(C:02)":49},"score":2491,"score_norm":0.963,"seq_a_single":27,"seq_ab_match":78,"seq_b_single":27}
ccagagagtcgggatgacgccaaggaacacgataaaagctgaggcaccggcgagtcgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatccgactctctgg
+
CCCCCCCC>C>A:>A@CCCCCCCCCCCBLEEEEEEEEBAEEBE5AAA8HU[OR[IZW[Z[\M@GT]`_RZ`e`Z_MSHISJPONM`Sa\aaa^`S_JbEEEBDEB?EDCCCCBCCCCCCBCC?CCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:68:10013:19657#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":61,"pairing_fast_score":0.914,"pairing_mismatches":{"(A:02)->(G:34)":104,"(C:02)->(T:35)":108},"score":3342,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggatgcctgttctaaggaggcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC=CC[ccca`c]^Zddbdd^eWeec^eebeeV^]\]`M[YZZJ`PSZRMRRQ`a_\_\[aL=EEMCCCCCCCCCCCCCCCCCCCCCCCCCCCDCCCCCCDCDDCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8625:7332#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(T:02)->(A:34)":99},"score":5174,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccagtgcaaggtagtactctggcgaataattttgtttttataattatttgtgtttagggctaagcatagtggggtatctaattgcactggagatcggaagagcgtttc
+
########A@>8A;A@C?eaececcaceeccbb^eeee_^edfeeeddddddddddddddeeeeeeeeeeefedebe_ccccaXZXYUc_cEEEEEEELEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8664:11185#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5045,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
cccacacacacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgtgtgtgtggg
+
CCCCCCedeV]P[\Ra^]^ZXVSY`cecebeeeeeebe^eeededdbedebddeefecae`Ncc[cbdcbedddeceeeeEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8679:17084#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":103,"pairing_fast_score":0.91,"pairing_mismatches":{"(C:02)->(G:34)":83,"(G:34)->(A:02)":6,"(T:02)->(G:34)":89},"score":4674,"score_norm":0.971,"seq_a_single":5,"seq_ab_match":100,"seq_b_single":5}
ccagagtacgcccattgagtctctgcacctatccttttcctttggattctagttcgagaaccacttgttttctcaaaacacggatttggctcaggattgcccgcgtactctgg
+
CCCCCLEEEc\\_e^c_cc[cada\eb_ddcdaQQSRIW\OdedZbcced]]`a\eab`cee_`beeabfddedaaeeceEELEEEEELEEEEECEEEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8655:7111#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":5623,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ccagagacgtgggatgacgccaaggaacacggtaaagctaaggcaccggcgagcctttcgagggctccgtcggctaccgaggccaacagaaaaaaatatgactctcggcaacggatatcgagcagtctgg
+
CCCCCCCCDCCCCCCCCCBCCCXededbeebccVc]\^_^Tca[YeeececYWc_eeeee_beeeaeda\_a]aY]cccccbebdabc^cZd_ZZ]dZ__ec^aeEEECCCCCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:9071:10042#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttccgct:ttccgct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttccgct","obimultiplex_forward_tag":"ttccgct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttccgct","obimultiplex_reverse_tag":"ttccgct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:02)->(g:34)":76,"(t:33)->(a:02)":34},"score":740,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaattattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCEEEBEEEEEEEEEEDKEEE=<<=>=EEECE;BEEEBCCEEE:Ece_aIbQVSWZN`ELBFEEADCCCCCCCCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8640:6135#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":3968,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
ccacgagacgtccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccccttttttttctcaaaacacggatttggctcaggattgcccacgtctcgtgg
+
CC@CCCCCEEEEEFEEEEEEcaa[PaKPQPRa_c`^`ZKb_^aec_^Yaa[I]ZSSbbUeZ`a^ebbaddce_dd`_cb\`cUaEEEEEEEEEEEEEEEEEEEEEEEECCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:10836#0/1 {"ali_dir":"right","ali_length":97,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":97,"pairing_fast_score":1,"score":3645,"score_norm":1,"seq_a_single":11,"seq_ab_match":97,"seq_b_single":11}
tcttccgatctcccacagcacaccattgagtctctgcacctatcctttttttattttcactttctgaacttttatttgtttcaggatttggctcaggattgccctgtgagatcggagga
+
###########EEEEEEEEEEEEEFEEEaW_a`__X_Z]_c_aadddddddddbdddd^beeecdaYRX\]eed_`dddedEEEEEEEEEEEEEEEBEEEEEEEEEEE###########
@HELIUM_000100422_612GNAAXX:7:1:8626:12273#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":92,"pairing_fast_score":1,"score":3802,"score_norm":1,"seq_a_single":16,"seq_ab_match":92,"seq_b_single":16}
ccaacattgattcggttggggcgacctaggaataatcatcctaaaaataaaagacaaattagtctatacaatgaacatttaaagatttaaaatcaagctaccttagggataacagcaatgttgg
+
CCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEa_]^\^\]Vc]d]ddeeXddddeeeeeca]ZV\[_cb_ccbZcURQQRQcddc^_cc^aEEEEEEEEEEEDEEFEEECCBCCDCCB@3;8988
@HELIUM_000100422_612GNAAXX:7:1:8641:12667#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":46,"pairing_fast_overlap":73,"pairing_fast_score":0.657,"pairing_mismatches":{"(A:02)->(C:34)":106,"(C:02)->(A:13)":81,"(C:02)->(A:34)":104,"(C:02)->(T:34)":102,"(G:18)->(T:02)":68,"(T:02)->(A:31)":107,"(T:02)->(C:34)":95,"(T:22)->(G:31)":75,"(T:26)->(G:02)":69},"score":1,"score_norm":0.877,"seq_a_single":35,"seq_ab_match":64,"seq_b_single":35}
ccacagcagctgcacggcatgtgccaaggaaaccaaaacttaagaagggtgcgtcacgtgttgtcccgttttcggtgtgcacacgggtcgtggcctctcattaaccataaacgactctcggcaacggatatcagctgctgtgg
+
DCCCCCCCCCCCCCCCCCCDCCCCCDCCBCB?BCBEECD=CCADDAEEEBECE8E4?:?CE/E/EA8<D5>;>7@X`D6C7?ACCCC5DEDEEALACCEBFLELELIEACCBCCCCBBCCCDCCDCCCCDBCCCBCCBDCCDC
@HELIUM_000100422_612GNAAXX:7:1:8652:14336#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":106,"pairing_fast_score":0.951,"pairing_mismatches":{"(A:02)->(G:34)":90,"(T:02)->(G:34)":108},"score":5473,"score_norm":0.981,"seq_a_single":2,"seq_ab_match":104,"seq_b_single":2}
ctccacgtactgcccattgagtctctgcacctatccttttttcgccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgcccgcagtacgtggag
+
CCc^]\\aUN\aKc\ddeI^\^Y^\\^^Wdefeedcedeeeeeb`a\aUeeeccWdeedddadcebd[dWedc^dc^Y\^`^_Y]aEEELEEEEEEEEEEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:8622:6541#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":5268,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
atctaggggcaatcctgagccaaatccgtgttttgagaaaacaaggggttctcgaactagaatccaaaggaaaaggataggtgcagagactcaatggctctctgtgggagat
+
:3ACddedc`dc]WXZ[YcKcc^ccbeedeeeeceedbeceeebdeeeeeeeadbe`adce`ebccccc\dcccecbabEEEEEEEEEEEEEEEEEEEEEEEEEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8666:15757#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":6065,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccgatctccgaatatcggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgagacttaggagatcggaagagcggttcagcag
+
####################BCCeeeeeeaeeZcaaScbcccc``accbeee]edeeeecefcdfdeedeedeeedeeeeeUeeeedeebebeeeeceebeb]___ccB@A####################
@HELIUM_000100422_612GNAAXX:7:1:8623:15978#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":105,"pairing_fast_score":0.784,"pairing_mismatches":{"(A:02)->(C:10)":93,"(A:02)->(G:27)":87,"(A:34)->(C:02)":16,"(G:02)->(T:25)":96,"(G:34)->(A:02)":13,"(T:34)->(G:02)":30},"score":2289,"score_norm":0.943,"seq_a_single":3,"seq_ab_match":99,"seq_b_single":3}
cccacagagaggggcaatcctgagccaaatccgtgttttgagaaaacaaaggggttctcgaactagaatacaaaggaaaaggataggtgcagcattgccccgtacacgtgg
+
CCCEEECEEDEDLEELEEEEEEEEEEEEDLEEEDEEEEEEbMSYaaUU]N[\SQ`U^YRPVcR[bb`WOZW`Z`\@[W[[L;T[8CE9D::<4,=C?<C<CCBBCCCCAAA
@HELIUM_000100422_612GNAAXX:7:1:8691:4410#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":73,"pairing_fast_score":0.714,"pairing_mismatches":{"(A:02)->(C:34)":106,"(A:34)->(G:10)":45,"(T:02)->(A:34)":104,"(T:02)->(C:34)":98,"(T:34)->(G:13)":59},"score":2385,"score_norm":0.918,"seq_a_single":35,"seq_ab_match":67,"seq_b_single":35}
ccacgcactaggcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatcctagtgcgtgg
+
CCCCCCCCCCCCC@CCCCCDCCCCCCCCBCCCCCC`deac`cdQFYUIYXZSTPKPPPEXWQSccb``ce`eeYeeedeDBEEEEBEDEDEEEDLEDLDECEELDLEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:7805#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":92,"pairing_fast_score":0.955,"pairing_mismatches":{"(A:02)->(C:34)":96},"score":2671,"score_norm":0.989,"seq_a_single":16,"seq_ab_match":91,"seq_b_single":16}
ccaattaacctgttatccctaaggtagctttattgatgatcttttaaagggtcagtaaatgataaatatatctatataggtgaggatgattaatccacggtcgccccaaccgaatgttaattgg
+
CCCCCCCCCCCCCCCCEEEFEEEEDEEEEEEEEEEcb`fKcbcceabeQdda\W^RQRQQYYZWZeeeeedfbfe_cEEEEEEEEEFEEEEEEEELEEEEEEEEEEEECCDCCCCCCCDDCCCC
@HELIUM_000100422_612GNAAXX:7:1:8696:8379#0/1 {"ali_dir":"right","ali_length":75,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":75,"pairing_fast_score":1,"score":5511,"score_norm":1,"seq_a_single":33,"seq_ab_match":75,"seq_b_single":33}
acactctttccctacacgacgctcttccgatctccagagtagcggggcaatcctgagccaaacctccgtcaaagagataggtgcagagactcaatggcgctactctggagatcggaagagcggttagcagaaatgccgaga
+
BCB<<?8>.?AA=@CCBAC>CCCCCCCC@ACB>eeeeeebeeeeeeeefeeeeeeeeeeeeeeeeedeeedefeeeeeeeebee`eeeeeeedeeeeeeb^ef`eceeCCB?;>:A6ACCB8CB@<@9-0'/0:<<)9AB?
@HELIUM_000100422_612GNAAXX:7:1:9232:2085#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acacgta:acacgta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acacgta","obimultiplex_forward_tag":"acacgta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacgta","obimultiplex_reverse_tag":"acacgta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":2227,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCBCCCDCCCCCCCCCCCCE@EEEEEEEeb]bQT_X^R_ee`\YX\]MY^[EaYabX\[ZabV`EDEECEEEEEEEEEEC?CCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8676:12836#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":83,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(C:35)":102,"(G:29)->(T:02)":38,"(T:02)->(G:34)":97},"score":3342,"score_norm":0.964,"seq_a_single":25,"seq_ab_match":80,"seq_b_single":25}
tccctacaccacgcgcttccgatctccagacatcgcgggcaatcctgagccaaatcctattttccaaaaacaaaggataggtgcagagactcaatggttgtcgtctggagatcggacgagcggtacagcagaa
+
#########################EEEEEEEEDAEEGc`Zccccece^]\\Yabc]]deaZd^\`badde]^``ec_c`][]a\ccaEEECDECELEDEEMEEDEEE#########################
@HELIUM_000100422_612GNAAXX:7:1:8671:16255#0/1 {"ali_dir":"left","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":84,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(T:34)":102,"(T:02)->(C:34)":104,"(T:02)->(G:34)":108,"(T:33)->(G:15)":43},"score":2994,"score_norm":0.952,"seq_a_single":24,"seq_ab_match":80,"seq_b_single":24}
ccaacgagcctgttatccctaaggtagcttgtttttgtactcttaaaatagggtctattagaggattaatatatctttgtaattaaggatgattattgtattccttggtcgccccaaccgaatgctcgttgg
+
CCCCCCCCCCCCCCCCCCCCCCCCEEEEEEc]`_^^]^L]a^C[X\\_a^`O_b`a^a]WabQa]VX_\adedbbb`YaEEEEEEDEEEEEEEEEEEEEEELELEEELCCCCCCDCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8639:17959#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5521,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccggtctccgcgcattcaagaagaccctatagagctttatctaaccatatgatatcatattcctagattcggttggggcgaccaatgcgcggagatcgcaagagcggttcagcag
+
#########A.>A=6:6@5)087^^a\IaUW^\_K^^_dbaeeeeeecab]beca____aaaaedcaac`fe]cbd\d_c^c][Z[\\b^aV_ced_adecZ_^aUaE#######################
@HELIUM_000100422_612GNAAXX:7:1:8632:15794#0/1 {"ali_dir":"right","ali_length":57,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":54,"pairing_fast_overlap":57,"pairing_fast_score":1,"score":4164,"score_norm":1,"seq_a_single":51,"seq_ab_match":57,"seq_b_single":51}
cggcgaccaccgagatctacactctttccctacacgacgctcttccgatctcagtcgtccgtatatacatacgtatacgtctgaatgaaagagttggtgagcggaaggagatcggaagagcggttcagcaggaatgccgagacggatatcgtatgccgc
+
#########################@?9>A>(A@CC>CAC@C@CCCCCCCCeeeeeebeeeeeeeedeeeeeeeeeeeeaeee`eeeeeedeeededeeeddcac^d^AB@################################################
@HELIUM_000100422_612GNAAXX:7:1:8666:3560#0/1 {"ali_dir":"right","ali_length":80,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":80,"pairing_fast_score":1,"score":4727,"score_norm":1,"seq_a_single":28,"seq_ab_match":80,"seq_b_single":28}
ctttccctacacgacgctcttccgatctccacgtgtacggggcaatcctgagccaaatcctgttttccgaaaacaaaggttcagaaagttaaaaaagggataggtgcaagatcggaagagcggttcagcagaaatg
+
##############BAA?0-0*07:<?6QQILQbeeZecYcXdbeedeeeee_eVaeeeeeeeeeeed\Z`ceecc\`deeeeec^ee[ac_acccdcEEEEEEEEEE############################
@HELIUM_000100422_612GNAAXX:7:1:8701:5058#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3878,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
gatacggcggccaccgagatctacactctttccctacacgacgctcttccgatctactagaatacaaaggaaaaggataggtgcagagactcaatgggacgagactgaagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgccgtcttc
+
###################?@9<CBC@B>:+:>A':@=?;>C@CCCCCBCDCCCCbeeeedecfeeeeeeeeeeeeceeeebeeceeeeeeedeeeeeedcceee]_c?AC;<AAC@BCB:;94<67778<3?5:00(00;?<)@00(1'8355645@7@=69
@HELIUM_000100422_612GNAAXX:7:1:9174:18106#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:agatcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agatcta","obimultiplex_reverse_tag":"agatcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(T:22)->(G:10)":35,"(T:34)->(A:02)":21},"score":1234,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
=AA8>AAACCCCCCCCCACELEa_dc]JX\V^UP:\\VDONMMW[\]=A5EDCD?4CEAEEEEEBE??C<@?EBEELEEEI?<?<:<C;CBCCCCCBC8C
@HELIUM_000100422_612GNAAXX:7:1:8692:21085#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":86,"pairing_fast_score":0.759,"pairing_mismatches":{"(A:32)->(G:02)":52,"(G:25)->(C:02)":64,"(G:33)->(C:02)":53,"(G:34)->(A:02)":25,"(T:33)->(A:02)":39,"(T:34)->(A:02)":33},"score":2147,"score_norm":0.93,"seq_a_single":22,"seq_ab_match":80,"seq_b_single":22}
ccagagagtcgggatgacgccaaggaacacggtaaagctaaggcaccggcgagcccttcgagggctccgtcggctgccgaggccaacagaaaaaaatatgactctcggcaacggatatccgactctctgg
+
CCCCCCC?CCCCBDCCCCCCCCEELEEEEEEELEEEEEKEEEEEEEEEBEDJKD2EEEEEA5<C/=C>A?baWebb^c]ZdcdK`MXNXQ]UWUMdZ[]]aS`^^aZ\CDCCCCCDCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8640:18047#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":105,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(A:33)":97,"(C:10)->(T:02)":19,"(G:32)->(T:02)":12,"(T:02)->(C:33)":104,"(T:02)->(G:33)":100},"score":2,"score_norm":0.952,"seq_a_single":3,"seq_ab_match":100,"seq_b_single":3}
ccacagctgacgggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggctgcatcgtgg
+
AAACCCC<BACJ0?A22242C@>@@>@@5@C>3BB9<@81<<;<><C%%%%CDDC>D@C@@<@@@@<@:@8D@AD8DC:DDA@5C511112@@:DAK?DKDDDKDDDDBBB
@HELIUM_000100422_612GNAAXX:7:1:8631:3991#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4896,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccacaccgtggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaacaatgttggagatcggaggagcggttc
+
###AB8?B;.9698?8C@addda]^^aWa\UbaReeefbbccbeedddeeeeeddeefee_ec]c`cceefee\aa___c__ZRRQQRc`aEEEEEEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8653:2995#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":90,"pairing_fast_score":0.828,"pairing_mismatches":{"(A:02)->(C:34)":104,"(A:34)->(G:02)":24,"(C:02)->(A:34)":97},"score":2646,"score_norm":0.956,"seq_a_single":18,"seq_ab_match":86,"seq_b_single":18}
ccaacaacaattcggttggggcgaccaaggaataattcatcctacacaacaaagacatctaagtctctccacagaccctaaaaaagatcatcaacaaagctaccttagggataacagtagaatcgg
+
CCCCCCCCCCCCCCCCCCEEEEELEEEEEEEEEEaU^]^UTd]cd`e_beReecaee_eaae[Zdcc__[befcDEEEDBDEEEEEEEEECEELEELEEEEEELEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8681:12562#0/1 {"ali_dir":"right","ali_length":12,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":8,"pairing_fast_overlap":12,"pairing_fast_score":0.889,"pairing_mismatches":{"(C:34)->(G:34)":97},"score":730,"score_norm":0.917,"seq_a_single":96,"seq_ab_match":11,"seq_b_single":96}
cgagaggcgtgtggattatatagcattgcaacccaaggtgcggccagcaagccagccgcgtccccgggttaggcacagtgttccttgacgccttcgscagagctctggatatccgttgccgagagtcgtgtggattatatagcattgcaacccaaggtgcggccagcaagcaagcggcgtccccgggttaggcacagtgtccct
+
#####################?=::9;3=3::A=?@+?AC@CCC:=CC6@CCCBA@CBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCeeeeeeeeedeCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCC=A;A=<2>><B<B:A9.9?############################################
@HELIUM_000100422_612GNAAXX:7:1:8785:19082#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (taatcat:taatcat)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taatcat","obimultiplex_forward_tag":"taatcat","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taatcat","obimultiplex_reverse_tag":"taatcat","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1302,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CBC8AAA=CCCDCCC@C;AcR__bee`ZaceeddZXecC@C4::=;6,3:9,3C>BEECEE4AEEEEEDEEEEEEELEEEECCCCCCCCCCCCC@CCCCA
@HELIUM_000100422_612GNAAXX:7:1:8698:17470#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5310,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
ccacgactctgccgaaggcgtcaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatcctctctcgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCaSca`aVXadffec\bca`d_cPc]]_X[QS]JMQVVLY`_]\Va_beeeeddeecc_\_adZZ]\\\\R[RS\^\Xa]`cced_CBCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8633:8169#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":105,"pairing_fast_score":0.775,"pairing_mismatches":{"(A:02)->(G:22)":93,"(A:34)->(T:02)":27,"(G:33)->(A:02)":19,"(G:34)->(T:02)":47},"score":915,"score_norm":0.943,"seq_a_single":3,"seq_ab_match":99,"seq_b_single":3}
ccagagacgtgccattgagtctctgcacctatcctttttgattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccccacgtctctgg
+
CCCEELEDDDLDD@EDEEKBEEEEBELEDD7CEBCCEEEEBEEDEELBBEBBA@7ISJOU[LSLKWbX^VYPQU1.;7DC<BBBDD89599=@,9==A>DDCDDDBDDBBB
@HELIUM_000100422_612GNAAXX:7:1:8676:17755#0/1 {"ali_dir":"right","ali_length":97,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":97,"pairing_fast_score":0.947,"pairing_mismatches":{"(C:34)->(A:02)":12,"(G:34)->(T:02)":19},"score":3707,"score_norm":0.979,"seq_a_single":11,"seq_ab_match":95,"seq_b_single":11}
tcttccgatctccagagagcacgggcaatcctgagccaaatcctatttttcaaaaacaaacaaaggcccagaaggtgaaaaaggataggtgcagagactcaatgggtgagatcgcacga
+
###########LEEEDEDLEDbc^YW[Y[P]aaa``c^__caXXaebSe`[```edO[__Zaa]YRPRRNO[Z[RQSS[ZV]cDBBD?ED?AEEEDEEEEEEDDA?A@###########
@HELIUM_000100422_612GNAAXX:7:1:8699:13136#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":85,"pairing_fast_score":0.878,"pairing_mismatches":{"(C:34)->(A:11)":29,"(C:34)->(G:18)":38,"(T:34)->(G:08)":31},"score":5382,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
ccgacacggctcgcgtccgatctccgcgcattggtcgccccaaccgaatttttgatcagtattattgatttattaaaaaagctcaatagggtcttcttgaatgcgcggagatcggacaagcggttcagcag
+
####AAAA76)7.;*9,;B@BABQQPRREKF]accaaDdcdbZebbdeeaeaecTeee`_`cdeeaeUbeeeecebeSeeaZeceecc\`abbeeeedebedbad_c[CC88@##################
@HELIUM_000100422_612GNAAXX:7:1:8644:1659#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5404,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacacgtcgcgcttccgatctccagctcgtctccattgagtctctgcacctatcctttgttttcttaacataaagatttggctcaggattgcccagacgagctggagatcggaagagcggttcagcaga
+
########################EEEEbXSc`]W[]Z^d\c`eceeceefdeeee_dbdebdedddeddeedeeededeeee_^cbbc^]cadbaace]\M]]SSZX5A9=8?##################
@HELIUM_000100422_612GNAAXX:7:1:8633:13747#0/1 {"ali_dir":"left","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":91,"pairing_fast_score":0.773,"pairing_mismatches":{"(C:02)->(G:33)":96,"(C:34)->(G:02)":23,"(G:02)->(C:34)":80,"(G:31)->(T:02)":54,"(T:34)->(G:02)":30},"score":332,"score_norm":0.945,"seq_a_single":17,"seq_ab_match":86,"seq_b_single":17}
ccacgcgcgatgatatccgttgccgagagtcgtgtggattaaatagcattgcagcacgaggtgcggccagcaggccggccgcttcctcgggctaggcacagtgttccttggcgtcatcctgtctg
+
CCCCCCCCCCCCCCCCCEEEEELEEEFEDLEDEEEEC>EEEE@EBDEEEE<-AI8:8<=;T_c`cCBC8ABEBEBE?EBLEEEDEEBBCCD>EEEKCBEEEDEEEEEECCCCCCCCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8774:6253#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:aacgagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgagc","obimultiplex_reverse_tag":"aacgagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:02)->(G:31)":56},"score":1911,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCAACCCCCCCCBCededeee^ceedeec]]dbOaX[accaaCC@CAEECIECLEAEEEEEEEEEBEEEEELDEFLCCCCCCCCCCCBC=CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8631:19858#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(C:34)->(G:26)":31,"(G:34)->(A:02)":14},"score":4248,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagcagtagcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggctactgctgg
+
CCCCCCEEEEEEELEEEEEEEe_caebee_Cceeeebee]e_aeeeeebdeeeabddeabeea^`eedeba^`YaZdca_a\cEEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:10108#0/1 {"ali_dir":"right","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":91,"pairing_fast_score":1,"score":6386,"score_norm":1,"seq_a_single":17,"seq_ab_match":91,"seq_b_single":17}
cgacgctcttccgatctccagacacgctccattgagtctctgcacctatcccatattacaaaaaagagtaacgtagggccttggctcaggattgcccagcgtgtctggagatcggaagagcggtt
+
62??@>6<==AA=8?AAccc`caee]dedaY]a_e\eecedbeefeeeeeeeeeeecddddeeddddddedbdbedeeeeecfcbadY[Z][`\]]^caca_cc]`bY9?@##############
@HELIUM_000100422_612GNAAXX:7:1:8688:9258#0/1 {"ali_dir":"right","ali_length":71,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":71,"pairing_fast_score":1,"score":4800,"score_norm":1,"seq_a_single":37,"seq_ab_match":71,"seq_b_single":37}
gactacactccttccctacacgacgctcttcctatctcccactagcgtccgaaggcgtcaaggaacaccccactgtcgactctcggcaacggatatcacgctagtgggagatcggaagagcggtcaagcaggaatgcggagaccg
+
#####################################EacX\baVedeebecdaebeceeceebee\edecddbaededaecebeeb]c\dabeafU_]cW\\ZW^\_@@@=.7+82AA>@########################
@HELIUM_000100422_612GNAAXX:7:1:9311:16890#0/1_sub[28..127] {"ali_dir":"left","ali_length":67,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (accaatc:)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"accaatc","obimultiplex_forward_tag":"accaatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_primer":"tagaacaggctcctctag","pairing_fast_count":58,"pairing_fast_overlap":67,"pairing_fast_score":0.906,"pairing_mismatches":{},"score":1887,"score_norm":0.97,"seq_a_single":41,"seq_ab_match":65,"seq_b_single":41}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCC@CCCCDCCEE__Q^b_`b`ceabd^ba_Xa`X\Q]X\^`DEBE=DEED>DEDEEEEDEEEEEEECEELELFEEECCCCCCCBCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:16349#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(A:34)->(G:02)":10,"(G:08)->(A:28)":67},"score":3579,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
tctacgggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggtgtgcactggaga
+
###EEEEEELDEEEEEDbQcacX_`_baccYccIbWa]beed`_cee_]_^_U\]aa[__a_c[\]@^cZa_aEEEEEEEEEEEEEEEEEEEEEEEEEEEFEEEEEEE###
@HELIUM_000100422_612GNAAXX:7:1:8622:14817#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":85,"pairing_fast_score":0.951,"pairing_mismatches":{"(C:02)->(T:34)":93},"score":3639,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
cccacagcacagatatccgttgccgagagtcgtgtggattaaatacgattgcaacacaagagatgaccagcaagctagccatgccctcgggttagacacagtgttccttgacgccttcggtgtgctgtggg
+
CCCCCCCCCCCCCCCCCCCCCDC^_[\W`ZIMcc\`UXVIV_`Z\^cd^a_ce^eeeafbd_`[YUdZe\VYPXQ^VX]RccLcEEEEEEEELEEEEEEBEEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8666:11535#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5038,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatattcgccagagtactaccgatattcggagatcggaagagcgattca
+
?A;:==A=3;.<84(,9:A`bdcccca^_ceea`P\__eR`^ee]eeecbeeedfeeeefeeeeceeaeeeeeee`ZeecdcbWa_ccc^cEEEEEEEEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8685:8972#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(G:34)->(T:08)":13},"score":6018,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
cccagactgtcgggcaatcctgagccaaatcctgttttctgaaaacaaccaaggattcagaaagttataataaaaaaggataggtgcagagactcaatgggacagtctggg
+
CCCVXV\^\\^aF]^_ac[cXeebMeaacaKc^ccaebbY`deaceedbeeebacedf_deeeeaeedddeded_d_d^dZYVU\a\Z_ad]]ZbEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8652:3958#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":105,"pairing_fast_score":0.971,"pairing_mismatches":{"(G:34)->(C:02)":6},"score":4638,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccactgacgtcccattgagtctctgcacctatcctttttgattttcgctttctgaatccttggttgttttcagaaaacaggatttggctcaggattgccctcacagctggg
+
CCCEELEEEEEEE`b_]b_e_]ZZI]Z`^^ZaKefe`bbaaceaeee`defefc`Z^^]aeebdeecaeZNRRQQcccY`ORRRJaRaEEEEEEEEEEEEEEEEEEFECCC
@HELIUM_000100422_612GNAAXX:7:1:8616:2526#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":103,"pairing_fast_score":0.84,"pairing_mismatches":{"(A:02)->(C:27)":101,"(A:02)->(G:33)":95,"(A:02)->(T:33)":98,"(A:33)->(C:02)":10,"(C:33)->(A:02)":9,"(C:34)->(G:02)":11},"score":284,"score_norm":0.942,"seq_a_single":5,"seq_ab_match":97,"seq_b_single":5}
ccactctgcacccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccttgttttctcaaaacacggatttggctcaggattgccccgtacgtctgg
+
@@A@@ABDKKLEEEE@D>DDEEEECECAEA@@CCB<BACBEAEEEEEEEDEEACA::>\ZNNMBO>>AA9AA7<DDDDA=9=ACCCACACAC8CKD>KACEADA;ACA6AA??
@HELIUM_000100422_612GNAAXX:7:1:8703:20089#0/1 {"ali_dir":"left","ali_length":80,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":80,"pairing_fast_score":0.753,"pairing_mismatches":{"(A:24)->(G:20)":83,"(C:02)->(G:33)":97,"(C:25)->(G:02)":71,"(C:30)->(G:02)":66,"(G:34)->(C:02)":31},"score":916,"score_norm":0.938,"seq_a_single":28,"seq_ab_match":75,"seq_b_single":28}
ccagtagctggatatccgttgccgagagtcgttttaaattcgacacgcgtccgatcccccgggtgcacgccgcgaacggggcaccgagggcgggcggcgttttgagtgttccttgacgccttcggcagctactggg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCABELDEEEEEEEEEE@EEEEEEDECA8?>AAAAEC=ABHA?@@CCCCC-8]UWX^:^\^G\W\YcZIYcKEEEEEEEEEEECC?CACCCC@BBCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8898:2731#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":2472,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCaace[a`^defecd^\\a_R[V]\aaa^\__V[]aWZaEEEFDEEEEEEEEEEEEEEECE?ECCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:9300:11652#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtaaca:tgtaaca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtaaca","obimultiplex_forward_tag":"tgtaaca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaaca","obimultiplex_reverse_tag":"tgtaaca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:26)->(a:32)":73},"score":1420,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataagctattctataacaaaataattcgccagagaactactagcaacagcttaaaactcaaaggacttggcggtgctttatacccct
+
CCCCCCCCC=CCCCBCCCEELEEEEEEEEEBEDDEDDBEDDEB@C@CCDBE=D?::C][XO_]dbbc]aab`A^X[Q]___ACC@CCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8735:17102#0/1_sub[76..81] {"ali_dir":"right","ali_length":60,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":60,"pairing_fast_score":0.789,"pairing_mismatches":{},"score":1121,"score_norm":0.933,"seq_a_single":48,"seq_ab_match":56,"seq_b_single":48}
cctagg
+
YYX[?S
@HELIUM_000100422_612GNAAXX:7:1:9051:12217#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(G:02)->(T:07)":56,"(G:34)->(A:29)":20},"score":1459,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccgtcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCD;A>AAAACCCCC?CCACRca_cZ^_bee_W\[^dLT`]^[aEEE8558*:;:18CEBEEEEEEEDCD:BEBFEEEEEBCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8840:1943#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aagacct:aagacct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aagacct","obimultiplex_forward_tag":"aagacct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aagacct","obimultiplex_reverse_tag":"aagacct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":2518,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEDDBDEAcX^`_[`bYLWRGbZ[_^`ed_Zdc\edeee]_ee_cZeEEECCCCCCDCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8677:14504#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(G:34)":100},"score":5344,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
cccagtgtgtggggcaatcctgagccaaatcgcgttttccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaggataggtgcagagactcaatggcacacactggg
+
CCC]ceebceaaeaeeee^cceceRecccc]eaeeceeTe_bdeedbcddee_debeccdceedcbdbdddedbdbbdEEEEEEEEEEEEEEEEEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8678:7615#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":101,"pairing_fast_score":0.959,"pairing_mismatches":{"(C:08)->(T:31)":93},"score":6932,"score_norm":0.99,"seq_a_single":7,"seq_ab_match":100,"seq_b_single":7}
ccgatctccagagctctgccattgagtctctgcacctatcctatttttgattcctttttgaattgctcgtaaaacaggatttggctcaggattgccccagagctctggagatcga
+
=96BBDCecedcedcaUcefddefdaeeeb_beeabdee`ddfeeeedeeeeeeeeecedeceeaXedeaeeeeeedbbebae_be_U]\\[Ccaa`\W___^`dZ^N?99:363
@HELIUM_000100422_612GNAAXX:7:1:8611:6236#0/1 {"ali_dir":"right","ali_length":75,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":75,"pairing_fast_score":1,"score":2748,"score_norm":1,"seq_a_single":33,"seq_ab_match":75,"seq_b_single":33}
tcgctctttcccgacacggcgctcttccgatctccagagtagcggggcaatcctgagccaaacctccgtcaaagagataggtgcagagactcaatggcgctactctggagatcggaagagcggttagcagcaatgccgaga
+
#################################EEEEEEEEEECBECEEEEEEECEEEEEDEb]W``XSUYVXaaaZZbb]P^[Q^X^KNHLR[a```aa[c`abTY`=>?##############################
@HELIUM_000100422_612GNAAXX:7:1:8810:3087#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aaccaag:aaccaag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccaag","obimultiplex_forward_tag":"aaccaag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccaag","obimultiplex_reverse_tag":"aaccaag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(a:08)->(g:34)":77,"(g:02)->(t:34)":81},"score":1620,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC?CEEDFCEEEEBEDEE?;?AC:5A=;CACCDCB=Cacc]][YZSGQccc`cb]e]c`a_F_DALCBCCCCDCCCCCC;CCCBD
@HELIUM_000100422_612GNAAXX:7:1:9341:18746#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gatctac:gatctac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatctac","obimultiplex_forward_tag":"gatctac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatctac","obimultiplex_reverse_tag":"gatctac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4206,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ccgtcta
+
deeaeed
@HELIUM_000100422_612GNAAXX:7:1:8625:9048#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":100,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:33)->(G:06)":39,"(C:02)->(T:34)":94,"(C:02)->(T:35)":102,"(G:31)->(A:08)":43,"(T:02)->(C:34)":92},"score":4168,"score_norm":0.95,"seq_a_single":8,"seq_ab_match":95,"seq_b_single":8}
tccgttgccgagagtcgtgtggattaaatagcattgcaacacgaggggcagctagcaagccagccgcttccccgggttaggcacagtgttccttgacgccttcgggactgacgtgg
+
CCCCCCCCR]S^Sc^Vabc_[R\MV\a^J]X`GRSZNYFXWUCJPWPZ=X_OZVaN`\^_[^^^Oabcc^dededTSUW`J[]^V]YaEEELFLDEEEEEEMEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8783:12344#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aaccgcc:aaccgcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccgcc","obimultiplex_forward_tag":"aaccgcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccgcc","obimultiplex_reverse_tag":"aaccgcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":62,"pairing_fast_score":0.831,"pairing_mismatches":{"(t:26)->(g:06)":39,"(t:33)->(g:08)":35},"score":2862,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCbDX_VJY[Y^a^aOQEKRS?J`HETPTQLJUKTSc]Z\aa`a^Sdcc_[``bUc`QaEBBCECCCCCCCCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8664:10550#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":85,"pairing_fast_score":0.866,"pairing_mismatches":{"(T:02)->(A:30)":104,"(T:02)->(C:30)":98,"(T:02)->(C:34)":95},"score":2447,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
ccactcgctacgcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatat
+
CCCCCCCCBCCCCBCCCCCCCCCEEEEEBEEEEDE?DEEEEEbccbbW[e\VKOOWREPYXVBP\Rdb_d[_aHWccZ`U`^Qc]EE=EEEEDEL:EHBEEEAHEEAA?A?CC@CCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8696:4045#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(G:34)":101},"score":5751,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
cccagctgatggggcaatcctgagccaaatcgcgttttccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaaagataggtgcagagactcaatggcatcagctggg
+
CCDCEEEEdceecZcc`^ZYWYecbdffefeeeefeceee`bdeedd]dbeedcdbedeeeeeee^accbddcccdddddWU[[PXYXVXaaEEEEEEEELEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:9164:4026#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(a:02)->(g:34)":77,"(c:02)->(t:34)":81},"score":3277,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCEEDEaZaU_cKNdUYYQX`bbeabdcd`^^]a`cdOdbcee^b]_V`[`cc`^_baELEEELCCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8612:11437#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":106,"pairing_fast_score":0.874,"pairing_mismatches":{"(A:02)->(G:34)":90,"(G:34)->(T:10)":37,"(T:02)->(C:34)":96,"(T:02)->(G:34)":108},"score":3881,"score_norm":0.962,"seq_a_single":2,"seq_ab_match":102,"seq_b_single":2}
ctcatcgaccattgagtctctgcacctatcctttttgattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccctcgatgcgtggag
+
##EEEECEEEccYQYYZ_aY\^^LQYVY[UWWIPNUFIWa]Yedb\dc_XW[SYY\Z_]\]Y`ce__[_ccbcYcccEECEEEEDEEEELEECDDLEEEEEDECEEEL##
@HELIUM_000100422_612GNAAXX:7:1:8609:10912#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":4957,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccacgctcatggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcatgagcgtgg
+
CCCCCCEEEEEEc[_aaaL\\Y]M^e^ee^_eeac_ccZdebdeeeedbdbeeeaeee^aea_eddecdccee]eaccdccZ_cEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8674:6245#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":102,"pairing_fast_score":0.929,"pairing_mismatches":{"(A:02)->(G:34)":106,"(C:02)->(T:34)":107},"score":5275,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccacgagacgtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccacgtctcgtgg
+
CCCCCCa[]c_Sdd\aUdc_ccc^cbbaedeedeae]eeeaadefbbef_`^eacaebbeeeefdddedeeddeb][aXafcEEEEEEEEEEEEELEEEEEEEEELLECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8659:18583#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":100,"pairing_fast_score":0.835,"pairing_mismatches":{"(A:02)->(G:34)":78,"(A:34)->(T:06)":28,"(G:28)->(T:10)":40,"(G:32)->(C:06)":13},"score":3030,"score_norm":0.96,"seq_a_single":8,"seq_ab_match":96,"seq_b_single":8}
ccagtacgagcgggcaatcctgagccaaatccgtgttttgagaaaaaaaaggggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggctcgtactgg
+
CCCCCCCCa]aZE\]Z\W[ed\cWScaGc]ZZNRLG]VS@VHRTY\^b^b^YeYedc\ccY\_dEEEEEEEEEEEEELEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8638:5453#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":89,"pairing_fast_score":0.837,"pairing_mismatches":{"(C:02)->(A:34)":95,"(C:02)->(T:34)":107,"(G:34)->(A:25)":29},"score":2399,"score_norm":0.955,"seq_a_single":19,"seq_ab_match":85,"seq_b_single":19}
ccgtgacgtattcggttggggcgaccaaggaaacatcatccttaaataacacagataactaaatctagcaatagacccttaactaagatcatcaataaagctaccttagggataacaaacgtcacgg
+
CCCCCCCCCCCCCBCCCCCEEEEEEEEcCcacZYZYW[V[]Leceeedeeffddbeeedc_dd]aECDEELEEEDEEEDEEEEEEEEEEEDEEELEEEDEEECEEELECCCCCCCCCCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8653:16644#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":101,"pairing_fast_score":0.969,"pairing_mismatches":{"(C:34)->(G:02)":8,"(T:02)->(G:34)":107},"score":3359,"score_norm":0.98,"seq_a_single":7,"seq_ab_match":99,"seq_b_single":7}
ccgatctcccacgtagttggggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatggagctacgtgggagatcgg
+
#######LEEDEc^a]Z^IaL^[eeebecYcKc]]``]eeceRbed]aecc^eeeedd_c_^aEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEELE#######
@HELIUM_000100422_612GNAAXX:7:1:8618:18384#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acacgta:acacgta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acacgta","obimultiplex_forward_tag":"acacgta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacgta","obimultiplex_reverse_tag":"acacgta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":2120,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCCCCCCCCCE@EFEEEEEEEDEEDDDCB@EE>D[]\[YY[BLaW]\T[c\cUJ_WW`S[^UX[`TNV_ccECBCCCCBBCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8652:13509#0/1 {"ali_dir":"right","ali_length":99,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":51,"pairing_fast_overlap":99,"pairing_fast_score":0.531,"pairing_mismatches":{"(A:02)->(C:34)":101,"(A:02)->(G:34)":104,"(C:02)->(A:34)":100,"(C:02)->(T:34)":86,"(C:34)->(T:02)":30,"(G:02)->(A:34)":103,"(G:02)->(T:34)":105,"(G:30)->(T:02)":70,"(G:32)->(T:02)":60,"(G:34)->(C:02)":68,"(G:34)->(T:02)":64,"(T:02)->(C:34)":87,"(T:02)->(G:34)":99,"(T:28)->(C:02)":56,"(T:34)->(C:02)":15},"score":46,"score_norm":0.838,"seq_a_single":9,"seq_ab_match":83,"seq_b_single":9}
tccctatctcccactcgtcaccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtacccaggatttggctcaggattgccctgacgagtgggaggtcggaa
+
#########EEEEELEEEEEEEDEEEEEELEEEEEEEEEEEEEELEEEEEDEFEEFEEDJEEELEE=LEH;a_cEEEEEEEEEEELLEEEDEEEEEBELLLELLLDEE#########
@HELIUM_000100422_612GNAAXX:7:1:8677:16780#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":104,"pairing_fast_score":0.881,"pairing_mismatches":{"(A:02)->(G:26)":94,"(G:02)->(C:34)":100,"(T:34)->(G:02)":42},"score":2601,"score_norm":0.971,"seq_a_single":4,"seq_ab_match":101,"seq_b_single":4}
cccagacgagtccattgagtctctgcacctatccttttcctttgggttctagtttgagaaccacctgtttttcaaaaaagggatttggctcaggattgcccgcgtcagctgg
+
CCCBEEEEEEEEEBEEEEEEEEEEEECEEEEEEBEEEEE=ELEDBEEdcc_ddbe[b^]Y`_e`_`ccXcd_`Zcccc^]`Ia^^aEBCCABEDEEEEELEEEEEEBECCCC
@HELIUM_000100422_612GNAAXX:7:1:8683:4332#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":85,"pairing_fast_score":0.634,"pairing_mismatches":{"(A:02)->(G:34)":96,"(A:32)->(G:07)":59,"(C:02)->(G:34)":91,"(C:02)->(T:34)":105,"(C:21)->(T:02)":30,"(G:33)->(A:02)":29},"score":1064,"score_norm":0.894,"seq_a_single":23,"seq_ab_match":76,"seq_b_single":23}
cccacgcagtcgatatccgttgccgagagccgtgtggattatatagcattgcaacacgaggggcggccagcgagccagccgcgtccccaagttacgcatagtgttccttgacgccttcgggactgcgtggg
+
@@)=@=??>BC@CCCCCCCBCC@EEDD5K??B6CBBBBB`ce]eeeRT`]][[W^OUNEC;CD>CD;E?EE@;AA?BLDEEEDLEEFDEELELEELEEEEEEEELEEECCCACCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8674:8621#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":102,"pairing_fast_score":0.879,"pairing_mismatches":{"(A:02)->(G:34)":96,"(G:02)->(C:34)":102,"(T:25)->(G:34)":90},"score":5657,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagagagcacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctcctagttttctcaaaacacggatttggctcaggattgcccgtgctctctgg
+
CCCCCC[]Wa^UU\aaUcc]caebc_cad]ccUcbeTeaecccc^e]edeefddccddbRebebeebbceeeee`_ddccedcb]__ccCaEEEELDEEDELEEEEFECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8684:8539#0/1 {"ali_dir":"right","ali_length":88,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":88,"pairing_fast_score":1,"score":5853,"score_norm":1,"seq_a_single":20,"seq_ab_match":88,"seq_b_single":20}
acacgacgctcttccgatctccaatgtcccaagaagaccctatagagcttttattcataaaatcacctaatagattttttaaattcggttggggcgaccggacattggagatcggaagagcggttcag
+
:=>=8=C;A>?<A3<9)9AA`d\c`ccXdcNd^_debddeeeedeeeddeedeeddeeeeeeedeeefedeedaeeeeeeeeeeaYXVZZccc\cPQRRRUYZXYU[Z88/-00):1<;:A=?A####
@HELIUM_000100422_612GNAAXX:7:1:8679:19748#0/1 {"ali_dir":"left","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":93,"pairing_fast_score":0.867,"pairing_mismatches":{"(G:34)->(T:02)":23,"(T:02)->(C:34)":97,"(T:02)->(G:34)":101},"score":3362,"score_norm":0.968,"seq_a_single":15,"seq_ab_match":90,"seq_b_single":15}
ccgcgcattctgttatccctaaggtagcttatgtttgtagtcttagtagggtctgttgattgattcattgatctttattatgagggatgatttatcctaggtcgccccaaccgaataatgcgc
+
CCCCCCCCCCCCCCCEEEEFEELEEEEEEbbcbaMa^]_`dedcaY][PP]^`a`bc_]cc_Zbd[Y_]__bccdeebaeEEEEFEEEEEEEEEEELEEELEEEEEEECCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8649:7771#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":105,"pairing_fast_score":0.98,"pairing_mismatches":{"(C:02)->(T:34)":107},"score":4996,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccagacgagaggggcaatcctgagccaaatcacgttttccgaaaacaaacgaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggctctcgtctgg
+
CCCa[_cccec[UYSY[[Va[a_TK`cccZadb`c_eeeddYdeeeZdca\Y^a`be\bedbedf`ceeaWY[Z^cadcEEDEEEEEEEEEEEEEEEEEEEEEEEELECCC
@HELIUM_000100422_612GNAAXX:7:1:8633:15209#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(A:34)->(G:10)":39},"score":5107,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccaatgtccttagataccccactatgcttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggacattggagatcgaaagagcggtca
+
0686:???<?A/A8CC?CRRRQOa]TaU[WLWUKS\[aFQQROX^Z^U_]__]eeeeceeeeeebeadabbcb]c^Z]US[[Z]a]]X^Y^]U]]^]]]a\]^XYZZUA=@###############
@HELIUM_000100422_612GNAAXX:7:1:8679:18656#0/1 {"ali_dir":"left","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":60,"pairing_fast_overlap":82,"pairing_fast_score":0.759,"pairing_mismatches":{"(A:02)->(C:34)":78,"(C:02)->(A:34)":98,"(G:29)->(T:02)":40,"(G:33)->(T:02)":42,"(T:29)->(G:02)":39},"score":747,"score_norm":0.927,"seq_a_single":26,"seq_ab_match":76,"seq_b_single":26}
ccgagctctgggatgacgccaaggaacacgataaaagctgaggcaccggcgagtcgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatccagagctctgg
+
CCCCCCCCCCCCBCBACCCCCCDBCCEEDBEDDEEDD@GGDK;CDEEE8D??C?TKVW^]GS[IR_T_\BE7EEE?ALAE@EBEDEEEE:LD?D<DELEECEEECECFCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8639:15912#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(G:34)->(T:06)":25},"score":5534,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccacgcgactaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccctagtcgcgtgg
+
CCCCCCRZ^_aLd]Zdbcec_cc\G[b^]_c_ZcdbabaeaebedeeeebbfddbdceaeeeceUbdee]\HQ^\[U]U\ee]ZSdcacY]bEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8613:18627#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":62,"pairing_fast_score":0.729,"pairing_mismatches":{"(A:02)->(T:06)":49,"(C:02)->(G:09)":56,"(T:20)->(G:02)":21},"score":66,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagttctaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
B>B7;8;3B@B<BC@?CC>>>ABAAEA@LXC@7,0<3A?8E7>AA>>10.2*BCE3B>A>A3=CCACEBDEEEELEICCEEBCCCCCCACCCB?3BBCAC
@HELIUM_000100422_612GNAAXX:7:1:8632:6804#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":103,"pairing_fast_score":1,"score":3212,"score_norm":1,"seq_a_single":5,"seq_ab_match":103,"seq_b_single":5}
ccacagctcgaccattgagtctctgcacctatccttttttattttcgctttctgaacctttgcttgttttcggaaaacgtgatttggctcaggattgccctcgagcgctgtgg
+
CCCCCEEEEEDEEEEEEEEEEEEEEEEEdZdaU]QZ]\Y_dcc]^\baddbdcdLW]YY\ZYY]ad`YcaT\N`aW][`@DDDDDDDD?DDDDDDDDDDDDDDDDDDDBBBBB
@HELIUM_000100422_612GNAAXX:7:1:8654:15870#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(A:02)->(G:34)":100,"(C:08)->(G:34)":55},"score":3750,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccactgtgctggggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggtgcgtctgtgg
+
BBBDB@@=@@@DBDDDDDADDdbd]aPQQMP]aNbbdbJccacddaXWYZQdS]FQUTZ[ddcc_XU]^^]`\^dbddN``abbEEEEEEEEEEEEEEELEEEEECEECCC
@HELIUM_000100422_612GNAAXX:7:1:8694:6465#0/1 {"ali_dir":"right","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":87,"pairing_fast_score":0.905,"pairing_mismatches":{"(C:02)->(G:34)":102,"(C:34)->(G:08)":39},"score":2747,"score_norm":0.977,"seq_a_single":21,"seq_ab_match":85,"seq_b_single":21}
cacacgacgcgcgtccgatctccaagacctcaagaagaccctatagagctttatcacacatatatcatattcatattcaacgattcggttggggcgaccaggtcttggagatcggaagcgcgcttcagc
+
#####################EEEEEEEEEEEEEaa``FR^I^ad\cbc\b`[e`eeeZeebeUeeede_aceecdEEECEEEEEEEEEEEEEEEE<EEEELDEEEEE#####################
@HELIUM_000100422_612GNAAXX:7:1:8613:13811#0/1 {"ali_dir":"left","ali_length":72,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":72,"pairing_fast_score":0.884,"pairing_mismatches":{"(G:02)->(T:10)":76,"(G:32)->(T:07)":58},"score":967,"score_norm":0.972,"seq_a_single":36,"seq_ab_match":70,"seq_b_single":36}
ccacgtagtcggcacggcatgtgccaaggaaaaagaaaacaaaagaaggttgtacccggtttttgccccgttcgctgtgtgcatgctggttgtgacctctttaacacaaaaacgactctcggcaacggatatccgactacgtgg
+
CCCCCCCCCCCCCBCCCCACCCCCCCCCCCCBC@CBDDDDEDDDDEDDEEBCBCaVZE`cccdZbaacb=4>=AE4EEDECCECEEDDECAAC,4ADCDDEEEED?EECCBCC?CCCCDCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8629:19308#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtggact:gtggact)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtggact","obimultiplex_forward_tag":"gtggact","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtggact","obimultiplex_reverse_tag":"gtggact","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(a:02)->(c:33)":80,"(t:31)->(g:02)":39},"score":1032,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCBCCCCCCCEIEFEBEEFEEEEEDDBDDIEEEBEAEEEEEC5EEBBBaa_cXJdbcbc`_Y]`EEEECEKECCCCCCCBCC>CCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8836:20887#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":1597,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCDCCCCCCCCCBCELECEEEEDEEEEEDDEEECECEE@>@C;;=A>@C`X`YUYLK_YYZQZa_Z__c_^Yc\XXCCDCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:57:10719:17535#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":1819,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggaggcctgttctagatattcgg
+
CCCCCCCCCCCCCDCCCCCCCCCBCCCCCCBCCCCCCCB;CBCCCCU[^dedececdcdcec^_e^_Wa_ccDEDCADEBDCEEEEEECEEEEEDE?EEEEEECEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8629:8033#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":108,"pairing_fast_score":0.924,"pairing_mismatches":{"(C:02)->(A:34)":94,"(G:32)->(A:20)":13},"score":4331,"score_norm":0.981,"seq_a_single":0,"seq_ab_match":106,"seq_b_single":0}
ccagatgcgacgggcaatcctgagccaaatcctattttttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatgggtcgcatctgg
+
EEEEEEEEEEEaBa_[__`\U_\eNecee]_d`eeecedc^dcddeedcbddee`daccec_bcc^bdbceee_eEEEEEEEEEEEEEEEEEELEEEEEEEEEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:9108:5324#0/1_sub[28..133] {"ali_dir":"left","ali_length":56,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:tatcagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatcagc","obimultiplex_reverse_tag":"tatcagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":56,"pairing_fast_score":0.925,"pairing_mismatches":{"(C:02)->(A:34)":65},"score":968,"score_norm":0.982,"seq_a_single":52,"seq_ab_match":55,"seq_b_single":52}
ttagccctaaacgtaaactgcaaactattccataataaaataattcgcccaagaactactagcaacagcttaaaactcaaaggacttggtggtgctttctacccct
+
CCCCCCCCCCCBD?CCCC@A@ABCACcedeecdbed^ecECEEEEEEADEDECEEEEEEEBEEELEEEEEEEEEEEDEEEECCCCCCBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8613:21124#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":3932,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ctacacgactctcttgcgatctccaaccgcccaagaagaccctatagagcttaatttaacctactaaataagtaaaaattaaattcggttggggcgaccggcggttggtgatcggaggagaggttgagca
+
######################EEEEEEEEEEDEEEEDd_`bbebaeebdddabebbcec]eedb`cbeeaccbddc\^[]^^`\M^][V[[aZ`aBEEEEEEEDEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8634:8458#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":104,"pairing_fast_overlap":107,"pairing_fast_score":1,"score":5331,"score_norm":1,"seq_a_single":1,"seq_ab_match":107,"seq_b_single":1}
tccacactgtcgccattgagtctctgcacctatcctttttgattttcgctttctgaacctttgttttcggaaaacgtgatttggctcaggattgccccgacagtgtgga
+
#EaRZTWTecbacc_`ccZeceed_ddZRQQRFSZXX\edXYeeeeeeeeeced__ad]cbeece_bbebceb\cbZ\N\``bYaCEEEEFEEEEEEEEEEEEEEEEE#
@HELIUM_000100422_612GNAAXX:7:1:8695:10876#0/1 {"ali_dir":"right","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":94,"pairing_fast_score":0.945,"pairing_mismatches":{"(A:02)->(G:34)":108,"(T:02)->(C:29)":73},"score":2988,"score_norm":0.979,"seq_a_single":14,"seq_ab_match":92,"seq_b_single":14}
cgctctttccatctccaatgtcctcaagaacataactaatactactcagacacaaaataacaattaacgctccaaaataatagggtatctaatcctagtggacattggagatcggaagagcg
+
##############D`V[]XZGVJ`^[_`aaaa[^^aaV\T_OR[]\X`X]^^Yaaaa^\]b^aEDDEEECEGBEDEEEEEDEEEEEEEEECEEEBEEEEEEEEEEEL##############
@HELIUM_000100422_612GNAAXX:7:1:8614:3877#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5163,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
cccacagacgtgggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggacgtctgtggg
+
CCDCCC^]]\a[a\\^^YK_Yc__Sebeee]edeefeeedecbeeedadddeeedeee`cceb`eeeeebc_c_caeeeUaEEEEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:6450#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(A:02)->(G:34)":84,"(T:02)->(G:34)":100},"score":4873,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccagatgcgacgggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatgggtcgcatctgg
+
CCCEEEaU]``Y\Zcb`^V^UcaeZedcbcdadNbceeTebddeedbcddaebbe_b_aededcd[^`b`a`[Y]WZ]LbEEELEEEEEEEEEEEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8674:16183#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":73,"pairing_fast_score":0.743,"pairing_mismatches":{"(C:02)->(A:34)":83,"(C:02)->(T:34)":102,"(G:02)->(A:33)":104,"(G:34)->(T:02)":47,"(T:02)->(C:34)":97},"score":1196,"score_norm":0.932,"seq_a_single":35,"seq_ab_match":68,"seq_b_single":35}
ccacgagagaggatatccgttgccgagagtcgtttatggttaatgagaggccacgacccgtgtgcacaccgaaatcgggacaacacgtgacgcacccttcttaagttttgttttccttggcacatgccgtgcctctctcgtgg
+
CCCCCCCCCCCCCCCCCCBCCCCCACCDCCC@CCCEEE?EBE@ECELaU[VWaYNSVYQSYZ[K_ZeeaBC9EEE@DEBEE=LEE?DDDEBEEBEDLEEEALBKDDDDCCCCBCCCCDCCCCCCCCCCCAC;CCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8634:1794#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":98,"pairing_fast_score":0.947,"pairing_mismatches":{"(G:02)->(A:34)":84,"(G:34)->(T:02)":11},"score":3885,"score_norm":0.98,"seq_a_single":10,"seq_ab_match":96,"seq_b_single":10}
ccagctagctggggcaatcctgagccaaatccgtgttttgagaaaacaaagaaaggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagctagctgg
+
CCCCCCCCCCLEEEEEc\^^[\a`Y`]\^_K_\c^YdbeddbbYcdZX\^ddc`de]_bccc__cde]be_[cacEEEEEEEELEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8766:5823#0/1 {"ali_dir":"right","ali_length":10,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":5,"pairing_fast_overlap":10,"pairing_fast_score":0.714,"pairing_mismatches":{"(C:34)->(A:34)":99,"(T:31)->(G:34)":108},"score":395,"score_norm":0.8,"seq_a_single":98,"seq_ab_match":8,"seq_b_single":98}
aggtcctttgagttttaagctgtggctcgcagtgttctggcgagcagttttgttgatttaactgttgaggtttagggctaagcatagtggggtatctamcgccggcggagaacaggctcctctagagggatatgaagcaccgccaggtcctttgagttttaagctgtggctcgcgctgttctgcggagccgttctcttattctaat
+
2CCA83ABC-CCCC<;CC;?C@A2CCCCCC@AC>?A==*A=AA8CA@@CCCBCBBCCCCCCCCC@CCCCCCCCCBCCCCCCCCCCBCBCCCBCCCCCCCeeeeeaUeCCCBBCCCCCCCCCCCC@?C@C?@CC<C@<<CC?A@=CC?>>CC?B98449?0?#############################################
@HELIUM_000100422_612GNAAXX:7:1:8629:13512#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":96},"score":4731,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccatgctgctgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccgtgcgcatgg
+
CCCCCCEEEEEEEd_cNcc\caZaNbeabebeeeccc]ceadeeebeeceeddfcfeedeeaeeddcedaeddddXZ]Z]aEEEEEEEEEEEEEELEEEEEDEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9283:4877#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tggtcct:tggtcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggtcct","obimultiplex_forward_tag":"tggtcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtcct","obimultiplex_reverse_tag":"tggtcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(T:34)":65,"(C:02)->(G:34)":56,"(T:32)->(A:07)":21},"score":2024,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCC2CCCCCCDCBCCCC;AbEb[Va^QObUc]e`cZe^Va\_X^]aYXYWb_[aELECEEEDEELELEEEEEEELELDCEECCCCCCCCCCCCC3CCCCB
@HELIUM_000100422_612GNAAXX:7:1:8860:19299#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:12)->(A:02)":29},"score":831,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataccaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
C@CCCCCCCCCCCCBCCBACCDCEEEED6222O^C[a]T_^`c]caE?:@=:@>+;<0BEA?CC?C@A<C?@DDCDF5CC<A@B?CCBCCCCC=@CCCCB
@HELIUM_000100422_612GNAAXX:7:1:8635:3805#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":4556,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccgcctccttcaagaacataactaatactactcaggcacaaaaattaacgctccaaaataatagggtatctaatcctagtaggaggcggagatcggaggagcgtttca
+
########BB5B@@B77@>`T]U\\\`M]YKaZ^bbbdbb\Ud`RVaaUXJddId_^ddd`d_\T]cc__a][[^^abRPTUXSNLQPPXXYRVba`DDADD@DB?DD###################
@HELIUM_000100422_612GNAAXX:7:1:8936:19499#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgcatt:gcgcatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgcatt","obimultiplex_forward_tag":"gcgcatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcatt","obimultiplex_reverse_tag":"gcgcatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:23)->(a:02)":41},"score":851,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCBCCCCCCCBCEELEEEEEEEEEEE@@E@@EEEAEAEEA:A?BEBB9?A?>caQPeUSI]K^SI]YaCDBB4EECCCCCCCCCACAC>CCCC
@HELIUM_000100422_612GNAAXX:7:1:8688:16859#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":73,"pairing_fast_score":0.786,"pairing_mismatches":{"(A:30)->(G:02)":43,"(G:32)->(A:02)":49,"(T:02)->(C:34)":97,"(T:34)->(A:02)":40},"score":525,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacatctgcggatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcgtttattgttttccttggcacatgccgtgccgcagatgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCACCCCCEDEELAAHD?ACCJ8CEEEEEEB=EEBEBXZ_UMVQTY]??AB@@C@CCCEE?EEEAEEEBLAEEEEEEEEDDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:11223#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":101,"pairing_fast_score":0.878,"pairing_mismatches":{"(A:33)->(C:02)":11,"(C:02)->(G:31)":98,"(C:02)->(G:34)":106,"(C:33)->(A:02)":12},"score":2776,"score_norm":0.96,"seq_a_single":7,"seq_ab_match":97,"seq_b_single":7}
cccacagcacaccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccctttttttttctcaaaacacggatttggctcaggatgccctgtgctgtggg
+
BBBBBBBDDDKKDD@BBDDDDDDDDDDDDDDBDDDDDDDDDADa^bRZba`_W_\bQ^U]b`aZ[OPHWTaSUQYZ]`Z`Zb_bbVbbbbEDDDEEEIEEEDCDELEECACAACC
@HELIUM_000100422_612GNAAXX:7:1:8876:5644#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agaaccg:agaaccg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agaaccg","obimultiplex_forward_tag":"agaaccg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agaaccg","obimultiplex_reverse_tag":"agaaccg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{"(c:02)->(t:34)":81},"score":2505,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagggtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCCCCCCCCC?CaOacbc_]`eebeZYRVYSUXVXSYWWZWT_QS`bbU`^]_[>EEEEEEDEEEEEEEEDBELCCCCCCCCCCC@C:CCCDC
@HELIUM_000100422_612GNAAXX:7:1:8681:9619#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":102,"pairing_fast_score":0.949,"pairing_mismatches":{"(A:02)->(C:34)":103,"(T:02)->(C:32)":108},"score":4749,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccacgtcgagtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccactgctagtgg
+
CCCCCCRb^___]ZVeUebc_`_XQQRNNKRGRP`[\eadccadcX[ca`e\_eVbecceebcY`\`ccY\T[c^[^]`a_cdaECDEEEEEEDEEEEEEEELEEECJCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8665:1944#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":108,"pairing_fast_score":0.952,"pairing_mismatches":{"(A:02)->(G:34)":108,"(T:02)->(C:34)":98},"score":5080,"score_norm":0.981,"seq_a_single":0,"seq_ab_match":106,"seq_b_single":0}
ccacgatgcagccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaaaataggatttggctcaggattgcccctgcatcgtgg
+
Ec__L__^c]dL`eec_c[c_cc__c_KdacaXeeceeeee\deeeeeebecddeedddadeedbdda]deedceEEEEEEEEEEEEEEEEEEEEEELEEEEEEEEEL
@HELIUM_000100422_612GNAAXX:7:1:8644:14473#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":108,"pairing_fast_score":0.771,"pairing_mismatches":{"(A:02)->(G:34)":108,"(A:08)->(T:02)":15,"(C:02)->(G:34)":81,"(C:02)->(T:24)":98,"(C:34)->(G:02)":1,"(C:34)->(T:02)":31,"(G:34)->(T:02)":4,"(T:29)->(G:02)":18},"score":2481,"score_norm":0.926,"seq_a_single":0,"seq_ab_match":100,"seq_b_single":0}
ccagatcgcgagggaaatcctgagccaaatcctttttttttttggcaaacaaataatgattccgaaagaaaaagggataggtgcagagactcaatggtcgcgatctgg
+
LEELE@EEEEEEEE2CBG<CEEEDEEEEDEL@EEEEEa]W`Y]Zc]Xa``ee`\aWETKaU\\WW_XY`bWZL]`[^T^aLEEEEEEEEDEEEEEEEBEEEDDEEEEL
@HELIUM_000100422_612GNAAXX:7:1:8638:18576#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":89,"pairing_fast_score":0.965,"pairing_mismatches":{"(A:02)->(C:34)":106},"score":3462,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
ccaacacactacaaccaaatccaatttcacgccaatattaaaaccaacaatccaaataaacaaatatcaatgtaatccatcattccacttagaaacaagctgcaccttgacctgaaatgtgtgttgg
+
CCCCCCCCCCCCCCCCCCCEEEEEEEEeeZeceeeeeZeeeeebeceeec]dd]ee`acdd`edcc_]cc^_\^[`EEDEEEEEEEEEEEEEEEEEEEEEEEEEELEEBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9022:7632#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgagt:ttcgagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgagt","obimultiplex_forward_tag":"ttcgagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgagt","obimultiplex_reverse_tag":"ttcgagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1841,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCC?CCCCCCCCCCCCCCCb^bddbdddddad^WNYSXRaa_`a__aCDEEDEEAEE2EDCEEEEEEEDEEEEDEELBEELCBCCCCCBCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8665:5972#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":90,"pairing_fast_score":0.897,"pairing_mismatches":{"(A:02)->(G:34)":99,"(C:34)->(T:02)":19},"score":4009,"score_norm":0.967,"seq_a_single":18,"seq_ab_match":87,"seq_b_single":18}
ccagcagcactgggcaatcctgagccaaatcctgctttccgaaaacaaaaaaataaaagtaaaagttcagaaagttaaaattaaacaaaaaaggataggtgcagagactcaatggagtgctgctgg
+
CCCCCCCCCCCCCCCCCCLEEEEEEcda___]^Nbcadca]dddddd^ccacba^\d\\]aaaa`\]___ddea^aadc`aT]]`EEEEEEECLEEEELEEEEEEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8873:1161#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aatgtcc:aatgtcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatgtcc","obimultiplex_forward_tag":"aatgtcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aatgtcc","obimultiplex_reverse_tag":"aatgtcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":63,"pairing_fast_score":0.767,"pairing_mismatches":{"(C:02)->(A:34)":58,"(C:31)->(T:02)":23},"score":242,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaacagattaaacctcaaaggacttggcagtgctttatacccct
+
CCB@CCCCBCCCCCCCC@B?CDIECEE?ECEa_R]B4CC<0@>;;=7=2,5=5578/LA?CA?B@ED<EBEBEEEL<E;LCCB?CC?CCCCBCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8680:12859#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5256,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccttatgtgactaggattagataccctattattttggagcgttaaattttgtgcctgagtagtattagttatgttcttgacacataaggagatcggaggagcggtgca
+
:<7<<@@ACC?C=76686?Kc_``_cdca_]dZ_cb`_feeeeeeeddeeeeeddeeedeeeeeeeeeeeeefc__aa[RRRRQQYXYX^^^^^ca`cEEEEEEEEFE###################
@HELIUM_000100422_612GNAAXX:7:1:8619:10468#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4530,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccagacatcgcccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgcccgcgatgtctgg
+
CCCEEEEEEEEEEEEEEEeeeaZZ\c]XY]]dSd_dac_XZefeebedec]\X\YWaX`aab^^Yddacc[\_a`_W__[YVXT[aM_aEEEEEEEEDEEEEEEEEEDBCC
@HELIUM_000100422_612GNAAXX:7:1:8645:14907#0/1 {"ali_dir":"right","ali_length":64,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"score":4552,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
caccgagatctacactctttccctacacgacgctcttccgatctccgaaggccctgttatccctaaggtagcttccaaccaagggtcgccccaaccgaatggccttcgagatcggaagagcggttcagcaggaatgccgagaccgatatcgc
+
###########################?1633A<A@>=A=9>:5eeeeeec]cdeeeeeedeeeeeee_eecebecb_ebdeafccceeed_eeeZee\\bWc]bb_bC;A0:B1C5CCC8A?A############################
@HELIUM_000100422_612GNAAXX:7:80:3343:6143#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":1407,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgtttctagatattcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCC@CCCCCCCCCCCCCC@DCCCaMZVdJd^SbabcbPYUY^cba[BD=BBDDDD<ADEDBFDEEEEDEB=EEEEEDEEEDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8701:14880#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":102,"pairing_fast_score":0.879,"pairing_mismatches":{"(C:28)->(T:32)":30,"(G:34)->(T:16)":22,"(T:02)->(G:34)":103},"score":4951,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
cccacagacgtgggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggacgtctgtggg
+
CCCCCC^]]`eXee\WZVUaaDcaSebeeAcc`^aaeebae`dedeeeaX_eeebeee^ceeaUbce`e`bcdcdac__\cQaEEEEEEEEEEEEEEEEEEELEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:3669#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":81,"pairing_fast_score":1,"score":5525,"score_norm":1,"seq_a_single":27,"seq_ab_match":81,"seq_b_single":27}
ccagagctctgggatgacgccaaggaacacgataaaagctgaggcaccggcgagtcgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatcctctctgtggg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCeceadeede]c\d`cYc_NX^X]e`ccbd_eabfeedeebbaebeececbe_eacdddcecde_U``bbXc_]]dbdU_U^CCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9114:9515#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgcca:ttcgcca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgcca","obimultiplex_forward_tag":"ttcgcca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgcca","obimultiplex_reverse_tag":"ttcgcca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{"(g:02)->(t:34)":81},"score":2836,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
C@ACCCCCC@ACCCACC@C?CAE?EDEEcc^c^`SYccb`__\^]]YNORMNOPaNT^\aSYa_T]V`PaaJ]Ya[YaEELCCBCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8683:13602#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(G:34)":103},"score":4774,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccatcgtcgcagggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtgcgacgatgg
+
CCCCCC\U^^dabaZQ]SVa]c^bbeedeedeee\``a\ddd_edeed_ddedceede`c`__U^_c`ccc_\bcUaEEEEEEEEEEEEEEEEEEEEEEEEELEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:5592#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":105,"pairing_fast_score":0.951,"pairing_mismatches":{"(A:34)->(G:23)":44,"(T:02)->(G:34)":108},"score":3890,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
tctcactgcagtgccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgttttcggaaaataggatttggctcaggattgccccactgcagtggaga
+
###EBEEEEEEEEEEEEEEb_dba]]N]e_eebd_deecdbcaCfeedeeeededeeedddddccbb;eceeeeecEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEL###
@HELIUM_000100422_612GNAAXX:7:1:8643:15574#0/1 {"ali_dir":"left","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":106,"pairing_fast_score":0.748,"pairing_mismatches":{"(A:34)->(G:02)":11,"(C:02)->(T:33)":108,"(C:34)->(A:02)":12,"(G:02)->(C:33)":102,"(G:34)->(T:02)":4,"(T:02)->(C:33)":104,"(T:02)->(G:23)":90,"(T:02)->(G:33)":96,"(T:34)->(G:02)":35},"score":1143,"score_norm":0.915,"seq_a_single":2,"seq_ab_match":97,"seq_b_single":2}
ccagctgagcaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccccgcgtag
+
CCELEEEEEDLLECEEEEEEEEEEEEEEEEEEEELEEEC@EEa`P[]a^aRNOQOLQSA?JFPDEUR^@@4<<+<<>A=AA<<<<98;:AA:A@:KD<DDDKDKDDDKBB
@HELIUM_000100422_612GNAAXX:7:1:9170:13596#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gatgacg:gatgacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatgacg","obimultiplex_forward_tag":"gatgacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatgacg","obimultiplex_reverse_tag":"gatgacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:34)":59},"score":1499,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCBCCCCCCC?CCCCCCdaY]S[Q]YOIWaGPMMIU\bc\a_Z]^?<AA<>>=A7>LAEE=DEEEEEEDEEEEELEDEBCBABB;CCCCCC@@CCCC=
@HELIUM_000100422_612GNAAXX:7:1:9400:2359#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acggtac:accaatc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acggtac","obimultiplex_forward_tag":"acggtac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"accaatc","obimultiplex_reverse_tag":"accaatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1621,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCCCC@CcWeeeeeb^eebae_U]c`V`[^aDEDDEDEEDCEEEE=EEEEEEEEEEEEBEEEEELEEELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8643:3847#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tggccac:tggtggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggccac","obimultiplex_forward_tag":"tggccac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtggc","obimultiplex_reverse_tag":"tggtggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"score":1659,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCBBCCC?CBCBB]^^eeba[be`^eac\`]U[\c_`aBDCEABABCEEDEBECEEEEEEEEDEEEDEEELDEELECCCACCCCCC@CCCCCCA
@HELIUM_000100422_612GNAAXX:7:1:8628:14956#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (taccgtg:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taccgtg","obimultiplex_forward_tag":"taccgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1889,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCDCCCCCC?Cb\dcceed^eedec[VZb_U]`\^aaaaEEBEBEEDBDABECEEEEDEECEEEEEEELCEELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8694:17147#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":106,"pairing_fast_score":0.806,"pairing_mismatches":{"(A:02)->(C:30)":101,"(A:02)->(G:33)":108,"(C:34)->(A:02)":33,"(G:34)->(T:02)":14,"(T:02)->(G:33)":97,"(T:34)->(G:02)":36},"score":839,"score_norm":0.943,"seq_a_single":2,"seq_ab_match":100,"seq_b_single":2}
ctccagatcgacggggcaatcctgagccaaatccttctttttggcaaacaaataaagattccgaaagaaataagggataggtgcagagactcaatggcgtcgatctggag
+
##EEEEB>A>A7ELEEEEEEDEEEEEEEEEEELEDLAEEE@?=@5DDBEEa\G^`D[VQ]UYX\bCDDDD>DD@DDDDDDDCDDDDDDDDDDDDDDKD/AHA>DACDK##
@HELIUM_000100422_612GNAAXX:7:1:8646:9541#0/1 {"ali_dir":"right","ali_length":50,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":43,"pairing_fast_overlap":50,"pairing_fast_score":0.915,"pairing_mismatches":{"(A:14)->(C:26)":72},"score":2482,"score_norm":0.98,"seq_a_single":58,"seq_ab_match":49,"seq_b_single":58}
taatgagttgtcatacaccgagatctacacgcattccgacacggcgctcttccgatcttcattagataccccactatgcctattcgccagagtactacctgagataggagatcgaaggagcggttcagcaggaatgctgagacagatatcgcatgccgtcttctgt
+
######################################################AB9>\XXIWZ[YSIOWM=MbVZV^`b`bbWZ]WWY_W^_PNIFNEQOQP``\`D##########################################################
@HELIUM_000100422_612GNAAXX:7:1:8664:4559#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(G:34)->(T:02)":8},"score":4423,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccactgtgctgccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgtttgttttcggaaaacgtgatttggctcaggattgccccagcacagtgg
+
CCCEEEELEEEEEDa\b_aecc]]U_c__``e^ebeceacfeeec_edeeee`eb_cbbcedbd_deddd[^]cdeeEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8638:4105#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(T:34)":107,"(A:34)->(T:02)":7},"score":4549,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
ccagagagtcgccattgagtctctgcacctatcctttatttattctcgctttatgaaacccttgtttgttttcataaaacgggatttggctcaggattgccccgactctctgg
+
CCCCCELEEEEEEFaR]`\b_R`bebZbebecb[Y`cZXV`PZa^XQWYYZa^XYaXT[Wd\I^\baeeed_dU`dad[aOQOQRdbccEEEEEECEEEEEEEEBELECACCC
@HELIUM_000100422_612GNAAXX:7:1:9374:4751#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agccata:agccata)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agccata","obimultiplex_forward_tag":"agccata","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agccata","obimultiplex_reverse_tag":"agccata","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(a:15)->(c:32)":65,"(c:24)->(a:02)":42,"(t:33)->(a:02)":35},"score":867,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CBCCCCCCCCCCCCCCCCCELBEEEEEEDDDDD?KCE:<<=B=EAEB?E@BEEBE>BEEEE:@_B[Y__eaacccXXZE5ECCCDCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:14174#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":73,"pairing_fast_score":0.829,"pairing_mismatches":{"(A:02)->(G:34)":89,"(C:02)->(T:34)":99,"(G:33)->(A:27)":46},"score":1973,"score_norm":0.959,"seq_a_single":35,"seq_ab_match":70,"seq_b_single":35}
ccacagctgacgatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcgtttattgttttccttgacacatgccgtgctgtcgctgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECadBYcaaXee^cc]^cbeaa[ddebUc^aZaEEEDEFAEEEEEECLE?EEEEEEELEEEEEEEDDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8841:8309#0/1_sub[79..81] {"ali_dir":"right","ali_length":56,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gccggcg:catctca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccggcg","obimultiplex_forward_tag":"gccggcg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"catctca","obimultiplex_reverse_tag":"catctca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":56,"pairing_fast_score":1,"score":3788,"score_norm":1,"seq_a_single":52,"seq_ab_match":56,"seq_b_single":52}
cgc
+
ddd
@HELIUM_000100422_612GNAAXX:7:1:8676:17529#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5919,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccgatctccgcgaacgcaagaagaccctatagagctttatctaaccatatgatatcatattcctagattcggttggggcgacccgttcgcggagatcggaagagcgcttcagaag
+
:75;?<:7CA@C@95''72><BA\cc`adZUeedeedebeceefeeeeeZeeeeeeeeeeaefbe_cUeefebeeeeebe`cY[\ZY`eebeedbe`b`b__c^\a\^?C@@###################
@HELIUM_000100422_612GNAAXX:7:1:8623:13575#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":100,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(T:34)":101,"(A:34)->(G:02)":16,"(A:34)->(T:02)":31,"(C:02)->(G:33)":98,"(C:02)->(G:34)":108,"(C:34)->(T:02)":33},"score":3514,"score_norm":0.94,"seq_a_single":8,"seq_ab_match":94,"seq_b_single":8}
tccgatctccagagtacgcgggcaatcctgagccaaatcttgtattattcagatgaatttcgggcgatgaggcgagatagatgcagagactcaatgggcgtactctggagatggca
+
########EEEEEEBLEDEEEEEEEEDEEELELEcacceZead^eedeQ`dcb[[a_^]TR_]a`]U\RPNMQ_\a__V[WTXS`aaa__ZYaEEEEKEELEEECEEL########
@HELIUM_000100422_612GNAAXX:7:82:4173:8120#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":61,"pairing_fast_score":0.914,"pairing_mismatches":{"(C:34)->(G:20)":52,"(T:02)->(A:34)":108},"score":3802,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ccgcctccttagaacaggcatcctctagaagggtataaagcaccgccaagtcctttgagttttaagctgttgccgctagtactctggtgaacaattttgtttgtgtaattatttgtgtttagggctaagcatagtggggtatctaaaggaggcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCDDCCACCCBCCDCCCCCCCCCC^`c`DcZcbbWebc^dec`d`aZabcbbcb^c[__]d^eaceeZ`d[aY`a`aXd_ecEELCCCCCCBBCCCCCCCCCCCCCCCCCCCCCCCCCBBCCCCCCCCC@CC
@HELIUM_000100422_612GNAAXX:7:1:8662:9796#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":85,"pairing_fast_score":0.78,"pairing_mismatches":{"(C:02)->(A:33)":90,"(C:02)->(A:34)":94,"(C:02)->(T:34)":102,"(T:02)->(C:34)":107,"(T:31)->(G:02)":33},"score":1148,"score_norm":0.941,"seq_a_single":23,"seq_ab_match":80,"seq_b_single":23}
ccagtctcgtcgatatccgttgccgagagtcgtgtggattatatagcattgcaacacgaggggcggccagcgagccagccgcgtccccaagttacgcacagtgttccttgacgccttcgggacgagactgg
+
CCCCCCCCCCCCCDCCCCCCCCCEEECEECEEIEBBCACCEEEDEEEAE?BCEEEEE=EE=?/3@,5LKY^YWZ[QdZ]dP^MQaaXbEKEEELEAEEECELEEEELECC@CCCCCCCCCCBCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8643:9202#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":105,"pairing_fast_score":0.931,"pairing_mismatches":{"(A:34)->(G:02)":6,"(C:02)->(T:34)":96},"score":5301,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccacgacacacccattgagtctttgcacctatccttttttattttcgctttctgaaccttcgtttgttttcggaaaacgtgatttggctcaggattgcccgtgtgtcgtgg
+
CCCEELEEcYc\ee_ecbM`c_`]`cacccceWdbcdbdbedddd^dcdeeee_ddcZddXXWZ[^^`YZY_]_aeebZbQORRScU_`aEEEEELEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:9352:16543#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:ttctaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttctaac","obimultiplex_reverse_tag":"ttctaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4493,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cctctaa
+
eeeeeee
@HELIUM_000100422_612GNAAXX:7:1:8631:5837#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":93,"pairing_fast_score":1,"score":5595,"score_norm":1,"seq_a_single":15,"seq_ab_match":93,"seq_b_single":15}
acgctcttccgatctccagagagcacgggcaatcctgagccaaatcccgttttatgaaaacaaagtgaaaagggggataggtgcagagactcaatgggtgctctctggagatcggaagagcgc
+
:?'>>A>A*<;;:CA]ccacdc]eeedeeeccecd_aee^eeeeefeeaeeeeebdddeeedddd`cc_cceeeeeeeec\ccZ]`aa`Y]Y[bDEEEEEEEEEEEEE###############
@HELIUM_000100422_612GNAAXX:7:1:9201:7576#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atgcaga:ggaggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgcaga","obimultiplex_forward_tag":"atgcaga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggaggaa","obimultiplex_reverse_tag":"ggaggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":3694,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cgcgaca
+
`aZd[\V
@HELIUM_000100422_612GNAAXX:7:1:8672:16331#0/1 {"ali_dir":"right","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":87,"pairing_fast_score":0.952,"pairing_mismatches":{"(G:34)->(C:18)":33},"score":5604,"score_norm":0.989,"seq_a_single":21,"seq_ab_match":86,"seq_b_single":21}
cacacgacgctcctcccatctccagagtacgcgggcaatcctgagccaagtccggcgaagggacacgagtagccggataggtgcagagactcaatgggcgtactctggagatcggaggagcggtgcaga
+
#####################c_]a]`a^\KUD`aaUadaeeeceUebceWeeeeeeeeee_ceec_`cccebceeeeaeedec]dcc_\`[Y[U[a^^YMZXVXZ__A??##################
@HELIUM_000100422_612GNAAXX:7:1:8612:6875#0/1 {"ali_dir":"left","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":83,"pairing_fast_score":0.95,"pairing_mismatches":{"(A:33)->(G:02)":34},"score":4212,"score_norm":0.988,"seq_a_single":25,"seq_ab_match":82,"seq_b_single":25}
cccacacagcagatatccgttgccgagagtcatattttttctgttgccttggccttggcaaccgacatagcccttgagcggctcgccggtgcctcagcttttatcgtgttccttggcgtcatccgtgtggtgg
+
CCDDCCCCCCCCCCCCCCBCCCDCCEEEEEEEEKEEEED`c``SeTdebebeaeXeee``^`c^]YYXYSZ[ZZZ^]]^^W__^_QNQRR_c]aY[]L`_cbba]c`cCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:7371#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":73,"pairing_fast_score":0.757,"pairing_mismatches":{"(G:30)->(T:05)":44,"(G:31)->(A:10)":53,"(T:02)->(A:34)":101,"(T:02)->(C:34)":95,"(T:29)->(G:07)":54},"score":2458,"score_norm":0.932,"seq_a_single":35,"seq_ab_match":68,"seq_b_single":35}
ccatgcgagacgcacggcatgtgccaaggaaaacaaaacataggaaggtcgcgtctcgtgatgcctcgttcgcggggtgtgcatgggtcgtggcctctcaataaccataaacgactctcggcaacggatatcgtctcgcatgg
+
CCCCCCCCCCCCCBCCCCBCCCCCCCCCBCCCCCCbKb\[a\bDMZ[QNOQTCBTW_`eec_ca]`]`c[Racbcdec_dE?CCEEEADEEEEELEEDEELEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9163:18791#0/1_sub[78..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tctgcat:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tctgcat","obimultiplex_forward_tag":"tctgcat","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4230,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
agag
+
ecee
@HELIUM_000100422_612GNAAXX:7:1:8680:7105#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":106,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(C:34)":97},"score":3987,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctcccacgctactccattgagtctctgcacctatcccgctttttattttcctttaactctttgttttcgaaaaagaggatttggctcaggattgccctcgtacgtgggag
+
##EEEEEDEEEbeIZWYUUc`Uc\^Y\aSUaa\]\\[L[`_`[eebb^\P\ea[`Y\bab`ee_e_[\^`beebbAEEEEEEEEEDEEEEEEEEEELEEEDEDEDEEE##
@HELIUM_000100422_612GNAAXX:7:1:8627:16516#0/1 {"ali_dir":"right","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":82,"pairing_fast_score":0.823,"pairing_mismatches":{"(C:02)->(A:34)":65,"(C:02)->(T:34)":82,"(G:27)->(T:02)":30,"(G:30)->(T:15)":63},"score":1395,"score_norm":0.951,"seq_a_single":26,"seq_ab_match":78,"seq_b_single":26}
ttccccacacagcgctgttccgatctccagatcgacggggcaatcctgagccaagtcccgtcggattttcggcgggataggtgcagagactcaatggcgctactctggagatcggaagagcgctctagcaggaa
+
##########################DDDE@@<<@@<@@]]cd`\add\TYYV^KQ`]ad`b@ELEEEBDAEDEEEEEEEELEEEEEEEEEEEEEEECECEEEDDEEE##########################
@HELIUM_000100422_612GNAAXX:7:1:8646:17023#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":4921,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccagcgtcatggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcatgacgctgg
+
CCCCCCZWRc`c`cWR[YRYPPVWMccee]baeeceeeeJ\IU\eeded`eefbbeee]beef]eeddedc_c_ccc`c`cKaEEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8688:1893#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":104,"pairing_fast_score":0.931,"pairing_mismatches":{"(A:02)->(G:34)":96,"(C:02)->(T:34)":99},"score":4840,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
ccagtcacgagccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccctcgtgact
+
CCCCEEEEEEEEEEEEEa`^Xab]_bd\c_c__eRe`dKebebceedeee\e_efXccc[ee_eb^eeceebded]cc\c\\YM^aacaEEEEEELEELEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8638:5204#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":85,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:34)->(G:02)":54,"(A:34)->(T:02)":30,"(C:06)->(A:35)":93,"(C:34)->(A:02)":31,"(G:34)->(T:02)":56},"score":2561,"score_norm":0.929,"seq_a_single":23,"seq_ab_match":79,"seq_b_single":23}
ccagagtacgcccgaaggcgtcaaggaacactatgcctaactcgtgggcgcggatggcttgccagccgccccatgcgttgcaatgctataaaatccacatgactctcggcaacggatatcgcgtactctgg
+
CCCCCCCCCCCCCCCCCCCCCCCEEELEELLEEEDFEEEEEEEEEE=CECEEELEL<=ECCEeWHR[c^_c^cP\^c]_`_\_[]W^Ua[X[H]]JVZYR`[[^adUeCCCCCCBCCCCCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8638:8252#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":89,"pairing_fast_score":0.826,"pairing_mismatches":{"(A:02)->(T:34)":94,"(A:34)->(G:02)":20,"(T:02)->(A:34)":84,"(T:02)->(A:35)":90,"(T:02)->(G:34)":107},"score":2089,"score_norm":0.944,"seq_a_single":19,"seq_ab_match":84,"seq_b_single":19}
ccgaagtagatttcaggtcaaggtgcagcttgtttctaagtggaatgatggattacattgtcatttggttatttggattattaattttaatattgatatgaaattggatttggttgtactacttcgg
+
CCDCCCCCCCCCCCCCCCBLEEEEEEEEEEEEEEEEFEa^_`RRRPTW^U\[cX][Y^^a_\]_cbb]c_aaaDEEEAEEEEELEEEDEMEEELEEEEEEEEEEFELECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8651:5513#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5142,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctctggcgcaggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaatgcgccaggagatcggaggagcggttca
+
AAA39;99C@CC>?===?=bb]abbdeaeeede_edcebddeeeefddeeeeedeeeeeeedeeceeeeefeefaaaU\a_`N\RQQRQcc]]cEEEEEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8689:17867#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5915,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcgtccgatctccggttcaccaagaagaccctatagagctttatcatcaatatattacctcgcatataataaagattcggttggggcgaccgtgaaccggagatcggaagagaggttca
+
CCC?43@CCC,>CB:>>B>^^W^__dd`\V^^TbZeaaececeef\eeUeeeddZbeeeededefeeef`bddddePdddcc_U\[^Z]^d^da]^[][a_aa_cKa\?A>=+=;?,@?########
@HELIUM_000100422_612GNAAXX:7:1:8653:10291#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":4784,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacacgacgctcttccgatctccagagctctggggcaatcctgagccaaatcctgttttctcaaaacaaaggataggtgcagagactcaatggcagagctctggagatcggaagagcggttcagcagaa
+
##############??:A@C3;CACbee[ca_`e]bbWa`Vc_Xebceeecdcbedbfeceeeac`\_bdeebZbcc`beab_Z`dccdceZZeEEEEDEEEEEEEEE#########################
@HELIUM_000100422_612GNAAXX:7:1:8642:17870#0/1 {"ali_dir":"right","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":79,"pairing_fast_score":1,"score":5652,"score_norm":1,"seq_a_single":29,"seq_ab_match":79,"seq_b_single":29}
tctttcccttccccgcgctcttccgatctccacgactctggggcaatcctgagccaaatcttgtttctacgacgagataggtgcagagactcaatggcacgcgtgtggagatcggaagagcggttcagcagaaatgc
+
#######################A*9==>^aceWbdeeaZcdcdeeebecfeeeeaed`eceeeeeedededddeeeefedee_]ccc_fdadebece^c_ccceee]CB@;3<@######################
@HELIUM_000100422_612GNAAXX:7:1:8627:3090#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (caacgcc:aacgccg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"caacgcc","obimultiplex_forward_tag":"caacgcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgccg","obimultiplex_reverse_tag":"aacgccg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{"(g:34)->(t:02)":55},"score":479,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaagctcaaaggacttggcggtgctttataccctt
+
CCCCACCCCCBCCCCCCCCELEEBEDEEEEEEBB5:=;<EE:B>??B7CC?ACALEEB7EEEZececObBCDEEDEECEEECCC?CCCCCCCCCBCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8680:7448#0/1 {"ali_dir":"left","ali_length":99,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":99,"pairing_fast_score":0.792,"pairing_mismatches":{"(A:09)->(T:02)":62,"(A:28)->(G:02)":11,"(C:15)->(G:02)":10,"(G:13)->(A:02)":51,"(G:18)->(A:02)":69,"(G:24)->(A:02)":50,"(G:28)->(T:02)":49,"(G:31)->(A:15)":76},"score":1476,"score_norm":0.919,"seq_a_single":9,"seq_ab_match":91,"seq_b_single":9}
cccacgtgacagggcaatcctgagccaaatcctgctttacgaaaacaagggaaagttcagtaaagaaagcgacgagaaaaaaaggataggtgcagagactcaatggtgtcacggggg
+
<<<22/0//9FBBBCCCCBCCCCCCCCCCCCCCBCCCC?CCCCCCCC?FB71*)1:>>7D:3@D@5:5<---PILAZ^[VZZZ[a`ZaaaPaZaaYK\Y_CC@@@D9633888/:>>
@HELIUM_000100422_612GNAAXX:7:1:8618:14843#0/1 {"ali_dir":"left","ali_length":77,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":48,"pairing_fast_overlap":77,"pairing_fast_score":0.649,"pairing_mismatches":{"(C:02)->(A:23)":106,"(C:02)->(G:34)":89,"(G:28)->(T:02)":40,"(G:33)->(A:02)":43,"(T:02)->(A:34)":100,"(T:02)->(C:33)":94,"(T:31)->(G:02)":55},"score":377,"score_norm":0.909,"seq_a_single":31,"seq_ab_match":70,"seq_b_single":31}
ctgcagtgcacggcatgtgccaaggaaaacaacatatgagaaggatgcatcctgttttgtcccgtttgcggtgtgcatacaggatgcggcctccttgaaatcacaaacgactctcggcaacggatatcagctgctgtgg
+
B<BB@??@@?>>>:>@A@@BBBBB>BBBBBB???DDDDDFD?K>D>>BB>D@D?IBD4B?@@A=@5;XTZQ^^^@DEED>A>9<B@E4L@D;?K=BEEELEEBECABB=@C8CCC@CCCCCCCCCCCCBCCBBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8664:14368#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":5598,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tgcctacgcgacgctcttccgatctccagagtagcgccattgagtctctgcacctatcccatcaggaatcctgaaaggacttggctcaggattgccccgctactctggagatcggaagagcggttcagcagga
+
#######################?@cea^Y_a_MYSYXZcdcZeeceeee]ace_eVdeedbbeee`edceeeeedecdea\aZcd_caVcd]^`^d^d[c_]d^a^_A?;:3?5B*B:?#############
@HELIUM_000100422_612GNAAXX:7:1:9193:1721#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agtcgtc:agtcgtc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtcgtc","obimultiplex_forward_tag":"agtcgtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtcgtc","obimultiplex_reverse_tag":"agtcgtc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{"(c:02)->(a:34)":73,"(c:02)->(t:34)":80},"score":1493,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCBCBCDCCCCBLDLELAEEEDEEEEDDEDD8BCAECEaaKLRJO\bYW^b][XX^ZU[a[^ORYaLEEEEBELECBCCCCCBCACCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8648:2906#0/1 {"ali_dir":"right","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":69,"pairing_fast_overlap":79,"pairing_fast_score":0.908,"pairing_mismatches":{"(A:15)->(G:27)":104,"(G:34)->(A:23)":32},"score":5314,"score_norm":0.975,"seq_a_single":29,"seq_ab_match":77,"seq_b_single":29}
tctttccctacacgacgctcttccgatctccggtcagcgtccattgagtctctgcacctatctcattttcaagataaaatttggctcaggattgcccacgctggctggagatcggaagagcggttcagcaggaatgc
+
##################AC<CCCA9ACBZdC_ee_ebeeeaaeee_ceeeedeeefedebbceeeeeeeebedeebeeeeede_cbaeec`_e_`cc]`]Y]=aZ\U?==::8>>8=?##################
@HELIUM_000100422_612GNAAXX:7:1:8645:1576#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(C:02)->(A:32)":104,"(T:33)->(C:02)":32},"score":2069,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
ccgacacggcgctgttccggtctcctgtaggtggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgacctacaggagatcggaagagcgctacagcag
+
#######################DDDDDDDDKCCADDDA__aZ_d]`VacV`ZaS^d^\_UOYS\[]V`aQVaEEBECEDEEBEEEDEEEEEEDDEEEEEDEEJEEEC#######################
@HELIUM_000100422_612GNAAXX:7:1:8669:12804#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":89,"pairing_fast_score":0.86,"pairing_mismatches":{"(G:34)->(T:02)":27,"(T:02)->(C:34)":99,"(T:02)->(G:34)":103},"score":1473,"score_norm":0.966,"seq_a_single":19,"seq_ab_match":86,"seq_b_single":19}
ccacgtaccctgttatccctaaggtagctttgtttgtgatcttaattaaagggtctgtaatttgatttattgatctttattatttaggatgatatttccttggtcgccccaaccgaatggtacgtgg
+
CCCCCCCCCCCCCCCCCCCEEECEEELEEEEEEEB`cS_becZT[ZY[RWIL^SYX]YR@Z[EDEEEEEBEFEEEEEEEEEEECCEEB5E@CEAEDEDLDEELEEDEECCDCCCCCCACCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8685:17048#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":75,"pairing_fast_overlap":103,"pairing_fast_score":0.75,"pairing_mismatches":{"(A:34)->(T:02)":26,"(C:02)->(T:34)":98,"(G:02)->(C:34)":101,"(G:34)->(T:02)":24,"(T:02)->(C:29)":64,"(T:02)->(G:35)":89},"score":1074,"score_norm":0.932,"seq_a_single":5,"seq_ab_match":96,"seq_b_single":5}
cccacacacacccatgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgtgtgtgtggg
+
CCCCCEEFEEEEEEELEEEEEEELELEEEEEEEEEEaV[_^[[ebeee`ZZ\aE=EEE@=A>5G@AECEEEE@E@EEEEEDEEEEDDEMEEDEEAEFLE7LEEDEEFDCCBCC
@HELIUM_000100422_612GNAAXX:7:84:12600:18313#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(A:02)->(G:34)":103,"(C:02)->(T:34)":107,"(G:02)->(T:07)":88,"(G:31)->(T:06)":63},"score":1033,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacatcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgtttctagatattcgg
+
DBCCCCCCCCCCCBCCCCCCCCCCCCCCCCBCCCCDCCCBCCCCAAEECce_ee\SZc_QRIDLH\Z\^B;B@CDABAD??6C@A@41ABCEAE?EAEEEEELAEELEBCCCCCBCCCCCC<CCC?CCCCCCCCCCCCCCCCCCCCCBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:7309#0/1 {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"score":4231,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
ggcgaccaccgagatctacactctttccctacacgacgctcttccgatctcagtcgtcccgtatatacatacgtatacgtctgaatgaaagagttggtgagcggaaggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgcgg
+
#?;=>=A>/0*5761>6)?7==A/8::8=74A?A>=CACCC=CCC;=CCCeddbbeaeededeeeededeaeeeeeeeeeeeeeceeeedeeeeeeedeecccccbeZCCC?;A;C;CCCCC4CC#################################
@HELIUM_000100422_612GNAAXX:7:1:8696:2892#0/1 {"ali_dir":"right","ali_length":12,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":9,"pairing_fast_overlap":12,"pairing_fast_score":1,"score":889,"score_norm":1,"seq_a_single":96,"seq_ab_match":12,"seq_b_single":96}
gttcatattttttctgttgcgttggccttggcaaccgacatagcccttgagcggctcgccggtgcctcagcttttatcgtgttccttggcgtcatcccagagctctggatatccgttgccgagagtcatattttttctgttgcgttgcccttggcaaccgacatagcccttgagcggctcgccggtgcctcagctttcatggtg
+
###########A>.:8=C92*274;3;'A>@@==:>CBB@CB@C?CCCACCC@CCCC>ACCCA?BCCCBCCBCCCCCCCCCCCCCCCCCCCCCCCCeeeeeeefeeeeCCCCCCCC@CCCCBBBC?C@C?CCDC@@@B@CCCCCC<A)A5A#####################################################
@HELIUM_000100422_612GNAAXX:7:1:8648:10572#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":81,"pairing_fast_score":0.795,"pairing_mismatches":{"(C:20)->(G:27)":81,"(C:32)->(G:09)":62,"(G:33)->(T:06)":58,"(T:02)->(C:34)":98},"score":1776,"score_norm":0.951,"seq_a_single":27,"seq_ab_match":77,"seq_b_single":27}
ccagacgcttcgatatccgttgccgagagtcatattttttctgttgccttggccttggcaaccgacatagcccttgagcggctcgccggtgcctcagcttttatcgtgttccttggcgtcatccgatgcgtctgg
+
CCCCCCCCBCCCCCCCCCCCCCCCCCCEEEEDEEEEEEB??@@E`eabZb``Wa[[ZFMOJDLJJWV[]DIPPPHGAOSW=[ZYQCC@CC<CCC@BBLEEBEEEEEEACCCCCCCCBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8639:5379#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tggtggc:tggtggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggtggc","obimultiplex_forward_tag":"tggtggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtggc","obimultiplex_reverse_tag":"tggtggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":2680,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
ACCCCCCCCCCCCC@C>CLEEaQZ]Ycc]]cQNMRMXMX\WRZcc_NRPKKVZUXXa\YZS[aXaa^acEEEEEECBECEECCCCCCCCCCCBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8642:19028#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacaggt:aacaggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacaggt","obimultiplex_forward_tag":"aacaggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacaggt","obimultiplex_reverse_tag":"aacaggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(C:33)":54,"(C:02)->(A:33)":59,"(C:02)->(G:34)":56},"score":1601,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCC@CCCCCCCCCCCCCCCcM\b`dbdb^addaXTUaXJZUVV]^aEEDBEDEKDLEEKECECEEEAEEEEEEEEELCEEECCCCCC@CCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9242:21037#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgacatc:tgacatc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgacatc","obimultiplex_forward_tag":"tgacatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgacatc","obimultiplex_reverse_tag":"tgacatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(G:23)":56},"score":1220,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttggccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CC;CCACDCCCCCCDCCCCBEAEEEEEEbbabd]RXcUW`\_a_c_cDADA:CDDACBEDEBEEEEEEEEEEEDEELEEELCCCCCCCBCCBCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8667:1343#0/1 {"ali_dir":"left","ali_length":75,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":44,"pairing_fast_overlap":75,"pairing_fast_score":0.611,"pairing_mismatches":{"(A:02)->(C:24)":94,"(A:02)->(T:26)":88,"(A:11)->(G:02)":71,"(A:32)->(G:02)":64,"(A:34)->(C:02)":41,"(A:34)->(G:02)":52,"(G:34)->(C:02)":45},"score":378,"score_norm":0.907,"seq_a_single":33,"seq_ab_match":68,"seq_b_single":33}
cctctgcggatatccgttgccgagagtcgtttttgtgttaaagaggtcacaaccagcatgcacaccgcgaacggggcaaaaaccgggtacaaccttcttttgttttctttttccttggcacatgccgtgccgcagatgtgg
+
CCCCCCCCCCCCCDCCCCDCCCCC@CACCCCCCBEDDEEELEEELE?BEEELEEEEBEEA?CCJC0CAC:5=>ZT\Z\Pa;>>DDDDDD>6=@B4;=<=3C<====A31A<:BBAABBB?<A06?ABBABB?<<??9???<
@HELIUM_000100422_612GNAAXX:7:1:8634:14506#0/1 {"ali_dir":"right","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":82,"pairing_fast_score":1,"score":3983,"score_norm":1,"seq_a_single":26,"seq_ab_match":82,"seq_b_single":26}
ctccccacacgacgctcttccgatctccaacaacatacaaccaaatccaatttcaattaacctgcccgttcttctgtttgctgcaccttgacctgaaatgccaccaggagatcggaagagcggttcagcaggaa
+
##########################EEEEEEEBEEEEEEFcSccZcc_`Z__```J\VU]acbcbcc_S`__^\c]cabUc[PZ[RO^^PY^MEETX]_YZb`VaBB##########################
@HELIUM_000100422_612GNAAXX:7:1:8611:11725#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":89,"pairing_fast_score":0.93,"pairing_mismatches":{"(A:02)->(T:34)":107,"(C:34)->(A:02)":32},"score":2245,"score_norm":0.978,"seq_a_single":19,"seq_ab_match":87,"seq_b_single":19}
ccgaatatcattcggttggggcgaccaaggaccacatcatcctttattacaaagaccattaagtcatacaaagacccttaaataagatcaccaaataaagctaccttagggataacaggatattcgg
+
CCCCCCCCCCCCCCCCBCCDEEEDEEEDECELEEECDe]Wde_eceaabdbac_\]c[ec\c^R_]a`_^EEEBEEEEEEEEEEEE?AEEADDEEEEEEEEEEEDELECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8955:5699#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgcca:ttcgcca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgcca","obimultiplex_forward_tag":"ttcgcca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgcca","obimultiplex_reverse_tag":"ttcgcca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:02)->(g:34)":77},"score":3208,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCELEEbeaddddfe``V_cU``\`_caa_[T[[\dddaeb^aVQM]HYY[baaU`aEELEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8653:13350#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aagacct:aagacct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aagacct","obimultiplex_forward_tag":"aagacct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aagacct","obimultiplex_reverse_tag":"aagacct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(c:02)->(t:34)":81,"(c:16)->(a:33)":68,"(c:18)->(a:34)":75},"score":1725,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CDCDCCCCCCCCCCCCCCCDLDEEEBEEDDDDDC=DDB8:^EBMQTIDGQNIHMN<IRda\Vb^acaCdeaZecDbJaEELCBCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:105:8082:13859#0/1_sub[29..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aatatct:gaatatc)","obimultiplex_forward_error":2,"obimultiplex_forward_match":"taggataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatatct","obimultiplex_forward_tag":"aatatct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(T:25)->(G:09)":37},"score":1405,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
CCCCCCCCCCCCCCBCCCEEBbeddfccWc[^AWSN=G\ZWb_ccE?;?@B4CCDCA<DD?=BEEEEEBEECEEELFECECCCCCCCCCCCCCCCDCC?
@HELIUM_000100422_612GNAAXX:7:1:8635:8777#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":54,"pairing_fast_overlap":73,"pairing_fast_score":0.771,"pairing_mismatches":{"(A:02)->(C:32)":97,"(A:02)->(C:34)":99,"(C:02)->(T:34)":91,"(T:02)->(C:34)":95,"(T:02)->(G:34)":86},"score":2339,"score_norm":0.932,"seq_a_single":35,"seq_ab_match":68,"seq_b_single":35}
ccatgcagtgcgcacggcatgtgccaaggaaaacaaaacataggaaggtcgcgtctcgtgatgccccgttcgcggggtgtgcatgggtcgtggcctctcaataaccataaacgactctcggcaacggatatcgcactgcatgg
+
CCCCCCCCCCCCCCCCCCACCCCCCCCCCBCCCCC`_c[\\`\`NSQaQ[[aOTPZIQTOUY\_H`_NV`RKVJddWYcbCC-CALEDEELBBELCJALEEEBEEEFECCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8707:4139#0/1_sub[74..81] {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgccgg:acacgta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgccgg","obimultiplex_forward_tag":"gcgccgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacgta","obimultiplex_reverse_tag":"acacgta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4567,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ccgatagg
+
eeeeeeee
@HELIUM_000100422_612GNAAXX:7:1:9090:20330#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgaacg:gcgaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgaacg","obimultiplex_forward_tag":"gcgaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":1,"obimultiplex_reverse_match":"tagaacaggctcctttag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgaacg","obimultiplex_reverse_tag":"gcgaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":62,"pairing_fast_score":0.814,"pairing_mismatches":{"(T:29)->(G:07)":36,"(T:33)->(G:09)":21},"score":1376,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCBCCCCCCB@C@BZE_Z`aaRIa_`aRHFBPVTQYYO`a`aEEEE>ECD>CEDE=ECECDEEDEEAEEEELDEELCCCDCCBCCCCCC<CCA;A
@HELIUM_000100422_612GNAAXX:7:1:9274:8709#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1817,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCACCCCBCCCCCCCCCBcZa^dccOPec^eed\Xc^[]]V\_b_]=9==5ECBE?BEEACBEEEEEEEEEEEEELBEED@CCCCCC?CCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8692:20649#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":103,"pairing_fast_score":0.88,"pairing_mismatches":{"(A:02)->(G:34)":86,"(T:02)->(G:34)":102},"score":4671,"score_norm":0.971,"seq_a_single":5,"seq_ab_match":100,"seq_b_single":5}
ccacgcgtatcgggcaatcctgagccaaatcccgttttatgaaaacaaacaaggatttcacaaagcgagaataaataaaggataggtgcagagactcaatgggcgacacgtgg
+
CCCCCa[^____]UX``\Jca_b]Xecca_cc_Z_ccccac_`ededcddcdaddccccdeceee```c`bcbcEEEEEELEEEELEEEEEEEEEEEEEEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8614:17184#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(C:34)":97},"score":4464,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
atctccacgcagctcccattgagtctctgcacctatccttttttctttttcaacttttgtttgttttcggaaaaccggatttggctcaggattgcccatcgcgcgtggagat
+
####EDDEEEEEEEEEEb`cUc^aaaa`\^[^\accc[[`d`ddebddbbddcaadeeddccddddd\beeefebec_ea`CEEEEEEEAEEEEEFLEEEEEEEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8683:17683#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":86,"pairing_fast_score":0.602,"pairing_mismatches":{"(A:33)->(G:02)":30,"(A:33)->(G:21)":48,"(A:34)->(G:02)":24,"(A:34)->(T:02)":23,"(C:02)->(A:32)":97,"(C:34)->(A:10)":71,"(C:34)->(G:02)":42,"(G:21)->(T:26)":53,"(G:34)->(A:02)":25,"(G:34)->(T:16)":61,"(T:30)->(G:26)":59},"score":1939,"score_norm":0.872,"seq_a_single":22,"seq_ab_match":75,"seq_b_single":22}
ccagctgacgcccgaaggcgtcaaggaacactgtgcctaacctagggacgcgtctggcttgctggccgcaccttgggttgcaatgctatataatcaacacgactctcggcaacggatatcgcgtagctgg
+
CBCCCCCCCCCCCCC?CCCCCCLLLE@EEKDDDEEEEDDEELECEaYCN[Z_<Ua\[[?VDUJWYJOORHF\c]c^`cTXXVM]Zab_bc_DEEECJC@CCAEEAEDEACCCCCCBCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8684:11165#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":103,"pairing_fast_score":0.95,"pairing_mismatches":{"(C:02)->(G:34)":102,"(T:34)->(A:02)":6},"score":2868,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
ccagatcgacggggcaatcctgagccaaatccgtgttttgagaaaacaagtggttctcgaactagaatccaaaggaaaaggataggtgcagagactcaatggcgtcgatctgg
+
CCCCCLEEEEEEEEEEEEEEEEEEEEEEEEEEEda^d^\7ZTOb_dc]ZdW^^X`eeXcba__a_bc]_cROQQJbaaZcEEEEEEEEEEEEEEEEEEEEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:9172:16041#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gatgacg:gatgacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatgacg","obimultiplex_forward_tag":"gatgacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatgacg","obimultiplex_reverse_tag":"gatgacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":62,"pairing_fast_score":0.678,"pairing_mismatches":{"(C:02)->(A:29)":59,"(C:02)->(A:32)":69,"(C:02)->(G:31)":56},"score":225,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCC?CCCC=AAAACCCACCDEDEEEEEE@@?@@=;:C?<>=>[aa`EEB5?DEABIE;G@A3?BEDBEJABBEIEDL:EELBC@CCCCACACC@=CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8659:13132#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(A:33)->(G:09)":15},"score":5442,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tcccatctcccacgacagtccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtaacccaggatttggctcaggattgcccactgacgtgggagatcgga
+
######A:ZbcbeYEZSXMWbdac\c_\c_cc`XWXU[^ceeeedeeedeeefeeebeeeceeeeeedd`cdcTa``T^^^^^ace`^a`aEEEEEEEEEEEEEEEDE########
@HELIUM_000100422_612GNAAXX:7:1:9065:10255#0/1_sub[28..133] {"ali_dir":"left","ali_length":56,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tggtggc:tggtggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggtggc","obimultiplex_forward_tag":"tggtggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtggc","obimultiplex_reverse_tag":"tggtggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":56,"pairing_fast_score":0.906,"pairing_mismatches":{"(c:34)->(a:02)":30},"score":647,"score_norm":0.964,"seq_a_single":52,"seq_ab_match":54,"seq_b_single":52}
ttagccctaaacgtaaactgcaaactattccataataaaataattcgccagaggactactagcaacagcttaaaactcaaaggacttggcggtgctttataccctt
+
CACCCCCCCCCCCACCDCCCCCCCCEELFLAEEEEECEC<9D9D`]da`eceb???A?EC=AEEEEBEDEEBEEEEEEEEECCCCCCCCCCCCCCCCCCCDCCCCD
@HELIUM_000100422_612GNAAXX:7:1:8613:8272#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":1458,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
tccgacaccgatagctacactccttccctacgccacgctcttccgatcgccaacgagcccgtatatacatacgtatacgtctgaatgaaagagttggtgtatggctggggatcggaagagcggttaagcaggcatgcggacaccgagcgcgtgcgca
+
#################################################cSabcc_QcbYb_V]X\]WVRXWR0:2><?.:CC;C9=9@@3@=ACCCACBCBCCCBCC#################################################
@HELIUM_000100422_612GNAAXX:7:1:8711:7668#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:aacgagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgagc","obimultiplex_reverse_tag":"aacgagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":49,"pairing_fast_overlap":63,"pairing_fast_score":0.817,"pairing_mismatches":{},"score":1071,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CBCCCCCC@CCDCCBCDC[be_ceedccdceddCAEEB?EAEEEEEEDEEEEDCDEELEDEDEEEEEEECEELEELCEFDECCCCCCCCCCCCCACCCC
@HELIUM_000100422_612GNAAXX:7:1:8696:10833#0/1 {"ali_dir":"right","ali_length":10,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":7,"pairing_fast_overlap":10,"pairing_fast_score":1,"score":740,"score_norm":1,"seq_a_single":98,"seq_ab_match":10,"seq_b_single":98}
cactgcagtgccattgagtctctgcacctatcctttttgattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccccactgcagtgccattgagtctctgcacctatcctttttgattttcgctttctgaacctttgtttgttttcggaaaacgtgatttgtctcaggattgccccactgcag
+
===1>;A@54+0;<6??8>;?=:78803C2@AC*45-8=A@A@AC>CAAA@==C<CB6ACB@@CCCCCCCDCCCCCCCCCCCCCCCCCCCCCCCCCCCeeeeeeeeeeCCC@CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC@CCCCCB?AA<BBCCA3:BAC>B7=8A?CCAA@############################
@HELIUM_000100422_612GNAAXX:7:1:9328:5341#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ggttcac:tgtattc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggttcac","obimultiplex_forward_tag":"ggttcac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtattc","obimultiplex_reverse_tag":"tgtattc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{"(A:34)->(T:02)":19},"score":1145,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCBCCCCCCCCBDCCBCLEEebee_We`\d^X^Q[\_5A1A<CC=DEECCEE>CEBEEEBEEEEEEEEEEEEEEECEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8646:17905#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":5142,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
accacgctcttccgatctccgcgaacgggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaacgttcgcggagatcggaggagcgtttc
+
####?:A>A9+AA<<<):]eeeedceee^^^^\_\U\`TbeecedZddeeeeeddeeeccedeeeffdfeeeedececee]c_XZYYXeeeeEEEEEEEEEECEEDEE##################
@HELIUM_000100422_612GNAAXX:7:1:8626:3393#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:02)->(G:34)":87,"(C:34)->(G:02)":25},"score":3558,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagatcgacggggcaatcctgagccaaatccatgttttgagaaaacaagggggttctcgaactacaatacaaaggaaaaggataggtgcagagactcaatggcgtcgatctgg
+
CCCCCCEEEEEEEEEEEEEEEEEELeedeZedede_eeebebbeece^[ddc`edd`ed_cebbdccae_caYaaEEEEEEEEEEELEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8697:12387#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":75,"pairing_fast_overlap":85,"pairing_fast_score":0.915,"pairing_mismatches":{"(G:28)->(T:05)":33,"(G:30)->(T:07)":30},"score":4047,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
cctacacgactctcttccgatctccgaaggccggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgggccttcggagatcggaagagcggttcagagg
+
<96<?<<0@?6=AAA=@C0?CCARRRRRRCKQB\WRWWZdeeaccccd]eee^eeeeeaeded``bbceeWeeaebebedbUeeeeaeEEEFEEEEEEEEEEEEEEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:9092:8244#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:ttctaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttctaac","obimultiplex_reverse_tag":"ttctaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4498,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cctctaa
+
eeeeeee
@HELIUM_000100422_612GNAAXX:7:1:8662:19405#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":103,"pairing_fast_score":0.8,"pairing_mismatches":{"(A:02)->(G:34)":90,"(C:02)->(T:29)":104,"(C:34)->(T:02)":6,"(G:02)->(C:34)":105,"(G:02)->(T:19)":83,"(G:34)->(T:02)":22,"(T:02)->(G:34)":108,"(T:34)->(G:02)":20},"score":3140,"score_norm":0.922,"seq_a_single":5,"seq_ab_match":95,"seq_b_single":5}
catctccagacgcatcccattgagtctctgcacctatcccttttttcactttctaaacctttgttttcgaaaaataggattttgctcaggattgcccgatgcgtctggagatg
+
#####LEEEEEEECEEEC?LELEEEcbb^K[^O[YXZY\ddZdbbb__ea\deaeedaX`_addabQbU^c\c_`@@BC?C?=CEEECDLEEEEEEDEDDEEEGLEEL#####
@HELIUM_000100422_612GNAAXX:7:1:9187:4782#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1195,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCACCCCCCCCCCBCB?Bb\aa_^^T_cYadd_`_aEEEEEADEEEBBECDEEDECECEEEEEEEEEEEDCEEEEL;CCFAA@C@@<?BBBCC>CCCC?
@HELIUM_000100422_612GNAAXX:7:1:8686:9051#0/1 {"ali_dir":"left","ali_length":99,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":99,"pairing_fast_score":1,"score":5418,"score_norm":1,"seq_a_single":9,"seq_ab_match":99,"seq_b_single":9}
ccagacgtacggggcaatcctgagccaaatctgtgttttgagaaaaaagaaaaggggttctcgaagtagaatacaaaggaaaaggataggtgcagagactcaatggcgtacgtctgg
+
CCCCCCCCCEEEEEEEc^F[Y[_^X`Xebcbdededdea[abdddd_dcd_`ddedecbee]eaea^_ca[c_cccac^dca`cZ^\Y[WYYJXZa\aEEEEEEEEEECBCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8679:8311#0/1 {"ali_dir":"left","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":94,"pairing_fast_score":0.934,"pairing_mismatches":{"(G:07)->(A:02)":36,"(T:02)->(C:34)":107},"score":1782,"score_norm":0.979,"seq_a_single":14,"seq_ab_match":92,"seq_b_single":14}
ccacgtcgtacgatatccgttgccgagagtcgtgtggattaaatagcattgcaacacgaggggcagctagcaagccagccgcttccccgggttaggcacagtgttccttggcgtcatccgta
+
CCCCCCCCCCCCCCEEEEFEEEEEEEEEEDC?DDC1=:=8EECEEEB?EBC?CDDDB:DbbbZ_UPUGKORSWXXJQSRM`SY[ZddX^c]aEEEEEFEEEEEEEELECCCCDCCCCCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8615:19485#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtggact:gtggact)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtggact","obimultiplex_forward_tag":"gtggact","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtggact","obimultiplex_reverse_tag":"gtggact","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(c:02)->(a:33)":68,"(c:02)->(a:34)":73},"score":735,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaagtaattcgctagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
C3BCCCCC?CCBBC?CBCLEADEE@EAEBB5A>)>WN>XROKVZ_\HSLU0=?=?C88><,BCCCECKACCBLE@ECE-ECCCCCCCC;C@?CCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8660:6397#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":4912,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
ccacagacgcagatatccgttgccgagagtcgtgtggattaaatagcattgcagcacgaggtgcggccagcaggccggccgcttcctcgggctaggcacagtgttccttgacgccttcggtgcgtctgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCEd_cdb\a]^a]Kce^ceeaeadb\SbeddeZbc[^d\Scc`Q_QZ]RRRPPKcUcZYa[Tc]b_c_ZcKLbLRQSOa`^dLXPaCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8666:13811#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":90},"score":4430,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
atctccactgcacgtccattgagtctctgcacctatcctttttttattttcactttctgaacttttatttgtttcaggatttggctcaggattgcccacgtgcagtggagat
+
##?6YZYZUU^^\UaU`\]_N__[^]][I][`]cddMc[d`dddcdddddedbbcdd]b]``dde^dbdadeeEEEEEEEEEEEEEEEELEEEEEEEEEEEEEEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8694:19583#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":102,"pairing_fast_score":0.929,"pairing_mismatches":{"(C:02)->(T:35)":106,"(T:34)->(G:02)":15},"score":4405,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccacgtgtacgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccccgtacacgtgg
+
CCCCCCEEDEEEEELa_eeeabecQQOKQcacWcebeeWecede`eebdbb^eXeee``eebdedd]ebeeb`eadee]eEEEEEEEEEEEEEEEEEFEEEEEEEMEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9046:16746#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aaccaag:aaccaag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccaag","obimultiplex_forward_tag":"aaccaag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccaag","obimultiplex_reverse_tag":"aaccaag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:28)->(g:02)":42},"score":1167,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaatacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCBCCCBCBCELCEEEEEEEEDEEDDEEBCCDFC>A9A9EEEEEAE4=EA<<92bb`c[[[O[[cbaca[XVCCCCCBC>CCCBCABCBCC
@HELIUM_000100422_612GNAAXX:7:1:8666:6654#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":6384,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccagacacgctccattgagtctctgcacctatccttttttcaccttctaggcctttgtttgtttttggaaaataggatttggctcaggattgcccctacgcagtggag
+
##EEEEEEEa^^\]UaK[`cc`cc__]_cZeee_beee\dacd\`]__e`eeeeddeedddcdeeddddTeeeedecX__cae^ceaQRJORXYYYZ`a_\\YPXYZ_AC
@HELIUM_000100422_612GNAAXX:7:1:8673:13558#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":101,"pairing_fast_score":0.622,"pairing_mismatches":{"(A:02)->(G:34)":96,"(A:16)->(G:17)":59,"(A:34)->(G:02)":11,"(C:02)->(G:34)":100,"(C:07)->(A:02)":51,"(C:14)->(A:19)":60,"(C:32)->(T:02)":33,"(G:32)->(A:02)":19,"(T:32)->(G:02)":15},"score":1325,"score_norm":0.891,"seq_a_single":7,"seq_ab_match":90,"seq_b_single":7}
ccatgcgctgaccattgagtctctgcacctatccttttcctttgtattctcgttcgaggacctccttgttttctcaaaacacggatttggctcaggattgccctcagcgtcatgg
+
301?>@@BBELJCCJACCJCCCCDCCCJCDCDJCEDEDEEDECEDEBEE11ONLFFNF36TT`@UW\^Nedabdaeee\dB?ACA?AC?C?BB@DLDEELDEEEEAEECC?CCCC
@HELIUM_000100422_612GNAAXX:7:1:8660:1373#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":92,"pairing_fast_score":0.865,"pairing_mismatches":{"(A:34)->(G:02)":20,"(T:02)->(A:34)":87,"(T:02)->(G:34)":104},"score":2740,"score_norm":0.967,"seq_a_single":16,"seq_ab_match":89,"seq_b_single":16}
ccatctctcatttcaggtcaaggtgcagcttgtttctaagtgaatgatggattacattgttatttgtttttggattgttgattttaatattgatatgaaattggatttggttgtacacataagg
+
CCCCCCCCCCCCCCCCEEELEEEEEEEEEFEEEEEEFFEFa^U^__]cZ`\\c^dbeeeaedddceeeddddc]d_c`\aEEFEEELEEEEEEEEEEEEEEEELEEEECCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8976:8917#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcctgg:ttcctgg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcctgg","obimultiplex_forward_tag":"ttcctgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcctgg","obimultiplex_reverse_tag":"ttcctgg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"score":1717,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCDCCCCCCCCBCCCBCDEEEEed]aedcdedb_d^a^Y]`\acaU^BDEEEEACEEEEBEEEEEEEEEEEEEELBECEECCCCCCCCCCCCCCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:20203#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":69,"pairing_fast_overlap":84,"pairing_fast_score":0.852,"pairing_mismatches":{"(C:02)->(G:30)":81,"(G:25)->(T:02)":37,"(T:06)->(C:02)":29},"score":1276,"score_norm":0.964,"seq_a_single":24,"seq_ab_match":81,"seq_b_single":24}
ccctacacgactctgttccgatctccagtacatgcgggcaatcctgagccaaatctttatgttaagaaaacaaaggataggtgcagagactcaatgggcatgtgctggagatcggaggagcgtttcagcaga
+
########################@@C@0BB?BBACCCB:4<6<aa_Uaa`XXaU]a]`aYVZ]B<??><854:B@6CBAHBCB;CC<CC?CCCCCCCCC<CCBCCCC########################
@HELIUM_000100422_612GNAAXX:7:1:8611:12943#0/1 {"ali_dir":"right","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":94,"pairing_fast_score":0.912,"pairing_mismatches":{"(G:34)->(C:07)":43,"(G:34)->(C:10)":39},"score":4082,"score_norm":0.979,"seq_a_single":14,"seq_ab_match":92,"seq_b_single":14}
cgctcttccgatctgtccacgaaccgcatgcacaccgcgaacggggctacacggcacgagtccttctcatttattgttatccttggcacatccgtgctcgagctgtggagatcggaagagcg
+
##############EEEd\[TZaVYZZJ_YecdRPQRRF[]YG[]\XX_dec^ccecdbe_c`ebbe_\ecccc^dedeQQGSRb[cEEEEEFEEEEEEEEEEEEEEE##############
@HELIUM_000100422_612GNAAXX:7:1:9057:20885#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (cgatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"cgatggt","obimultiplex_forward_tag":"cgatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":48,"pairing_fast_overlap":63,"pairing_fast_score":0.8,"pairing_mismatches":{"(c:02)->(a:34)":75,"(t:26)->(a:07)":35},"score":1390,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggtggtgctttctacccct
+
CCCCCCCCBCCCCCC=C=CELELa`a_W\]__SZ?X\SUZ_ZLZXWUCUA><AAAAA7>A9<EDEEEEEEEEEELEEBEEEECCACCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:41:6432:11693#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":44,"pairing_fast_overlap":61,"pairing_fast_score":0.759,"pairing_mismatches":{"(A:02)->(G:35)":104,"(C:02)->(A:31)":86,"(C:02)->(T:34)":108,"(T:32)->(A:26)":48},"score":2164,"score_norm":0.918,"seq_a_single":47,"seq_ab_match":56,"seq_b_single":47}
ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagccctgttctaaggaggcgg
+
CC>CCCCCCC>CACBBCCCCCCCCCCCC<?=BBBBCBCCCCC@B?ACA`c_ec\Vc`cY_YTZ\KUa[^ac]\_P]^Z[^Z^T]aIEFDEELEEEEEAEEEEEMCEFLCDCCCCCCCCCCC@CCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8621:7114#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":103,"pairing_fast_score":1,"score":4556,"score_norm":1,"seq_a_single":5,"seq_ab_match":103,"seq_b_single":5}
gatctccacgactctggggcaatcctgagccaaatcctgaaacaaataaaagttcagaaagtgaaaataaaaaaggataggtgcagagactcaatggcagagtcgtggagatc
+
>7<;AMcc^]IY\ebeab^``\^LXXYY_b_dbb_cec`\cedddddfedbdadbX[YWW`X][YddecddcdcdEEEEEEEEEEEEEEEEEEEEEEEEEEEDEEEEE#####
@HELIUM_000100422_612GNAAXX:7:1:8648:16601#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":85,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:34)->(G:12)":54,"(G:30)->(C:19)":63,"(G:34)->(T:02)":36,"(T:02)->(C:34)":107},"score":648,"score_norm":0.941,"seq_a_single":23,"seq_ab_match":80,"seq_b_single":23}
ccagtgcgtgagatatccgttgccgagagtcgtgtggattatgtagcattgcaacccagggtgcagccagcaagccagccgcgtccccgggttaggcacagtgttccttgacgccttcggtcacgtgatgg
+
CCCCCCCCCCCCCCCCCCCCCCCEEEEEL=CEEEELEEEBEDECDBCBC_cZaE_\]WRYVZ@CADEECEEEEEEAEFEEDEDEEEEEEEEEEEEEEEEEDEEEEELECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:89:16245:7643#0/1_sub[78..81] {"ali_dir":"right","ali_length":55,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:tcctcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tcctcct","obimultiplex_reverse_tag":"tcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":55,"pairing_fast_score":1,"score":4065,"score_norm":1,"seq_a_single":53,"seq_ab_match":55,"seq_b_single":53}
tttt
+
eeee
@HELIUM_000100422_612GNAAXX:7:1:9018:1264#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtacag:aacgccg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtacag","obimultiplex_forward_tag":"tgtacag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgccg","obimultiplex_reverse_tag":"aacgccg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1637,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC@CaI\^^`aOV_\\cc]KYdKP]\Va[___8<?-<BEDEEBEEECEEEEEEEDEEEEEEL?E?ECCCCCCBCCCCCC=CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8655:10865#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":108,"pairing_fast_score":0.962,"pairing_mismatches":{"(G:34)->(A:02)":6},"score":4578,"score_norm":0.991,"seq_a_single":0,"seq_ab_match":107,"seq_b_single":0}
ccagagctctgccattgagctctgcacctatccttttcctttgtattctagttcgagaacccccttctcaaaacacggatttggctcaggattgccccagagctctgg
+
EEEEELEEEEEac_[Y\^JQQRR_ac__cd_aeca^eeeeeedeedeedecdedadedcbacdabaebb^eeeeb]]WaEEEEEEEEEEEEEEEEEEEEEEEEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:8700:13996#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":97},"score":4869,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tgcgatctccagagtacgcgggcaatcctgagccaaatcttgtcttactcaaatgaatttcgggcgatgaggcgagataggtgcagagactcaatgggcgtactctggagatcgca
+
;97?C@@Cc]ccc[VIX\da`cccaa^__d]\\e]Zeebeeecceebcc[eeeccceeeebcdebcU_\a\Vaceecc_aEEEEEEEEEEEEEEEELEEEEEEEEEFE########
@HELIUM_000100422_612GNAAXX:7:1:8686:20217#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(C:02)->(A:28)":103,"(T:32)->(G:02)":41},"score":1778,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
ccaaccgccattcggttggggcgaccaagggatacaataatcaccctaaactgcaaagatacattaatccacaaaagaccctaattttagagcataaaaacaagctaccttagggataacagggcggttgg
+
CCCCCCCCCCCCCDCCCCCCCCCEEEEEEEEEEEEEEEEEJEEEEEDFEEEFDBDEEEDFCCC=C@^[Yb`c_ccdSHYSWPST]XddV^\cXU^@@EEEEAFA?A@DB@@CCCCCBCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8640:12181#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":3972,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
ccactagctcgccattgagtctctgcacctatccttttttgattttcagtttctgaacttttatttgtttgttttcggaaagaaggatttggctcaggattgccccgagctagtgg
+
CCCCCCCCEEEEEDEEEcU`cZXSVX`X[]]caeee_`_`Ybeeeeebeeeeecd]cacdeedb`dccdbdfedaEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECEECCDCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8659:1473#0/1 {"ali_dir":"left","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":94,"pairing_fast_score":0.89,"pairing_mismatches":{"(C:02)->(T:30)":107,"(G:02)->(C:34)":90,"(T:02)->(G:34)":104,"(T:34)->(G:02)":15},"score":3659,"score_norm":0.957,"seq_a_single":14,"seq_ab_match":90,"seq_b_single":14}
ccagacatcgcccattgagtctctgcacctatcctttatgtttatttttgctttctgaagcccttttgttttgtttgttttcggaaaaccggatttggctcaggattgcccgcgatgtctgg
+
CCCABC@CCCCCCBLEEEaaacT]`XY]c_[Z]ddad^aXOHSCX_SBR\_a_eeb\[Rb\_WbQaca[N_`W`JZ`bc?EDEEEEDCBLEECEEEEEEEEEELBEHCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8677:7487#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":92,"pairing_fast_score":0.753,"pairing_mismatches":{"(A:02)->(C:32)":102,"(A:02)->(T:34)":104,"(A:33)->(G:08)":44,"(A:34)->(T:02)":34,"(C:02)->(A:34)":92,"(G:30)->(A:02)":29},"score":995,"score_norm":0.935,"seq_a_single":16,"seq_ab_match":86,"seq_b_single":16}
ccttatgtgattcggttggggcgaccgtggattaatcatcctcacctatatagatatatttatcatttactgaccctttaaaagatcatcaataaagctaccttagggataacagcacataagg
+
CCCCCCCCACCB?C?CEEECEECEAAD@H:BBELBE@EAA4B\EXU\^Xc]^bZeeaee11222:5;;6EEB@CEEBAEEEEDEEBEEBEELEEEEEEECEJBLEEEECCCCCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8648:19761#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(A:02)->(G:34)":84,"(T:34)->(G:10)":36},"score":3975,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccagacgtacggggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggcgtacgtctgg
+
CCCEEEEEEEEEEEEEEaT_[_d`cca_cXcR[WUF[T[\YTYc[[[_c`_addeX]\_b^]eeedbbddd^]ac`_d_aEEELEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8663:19202#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":5906,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacccgacgctcttccgatctccagagtagcggggcaatcctgagccaagtcctttcaggattcctgatgggataggtgcagagactcaatggcgctactctggagatcggaagagcgcttcagcagaa
+
?AA8-03*??<C=ACC?A?99?:B8_bcdacdcee^eedeeeeeeeeeebeXdeeeedefeeeedaeeeee`ddeebfeccWd_bbdddbeceee_\ddUc__cc_caA@<:+;<;3<:?#############
@HELIUM_000100422_612GNAAXX:7:1:8675:8792#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":108,"pairing_fast_score":0.924,"pairing_mismatches":{"(A:02)->(G:34)":108,"(A:02)->(T:34)":93,"(G:02)->(C:34)":96},"score":5301,"score_norm":0.972,"seq_a_single":0,"seq_ab_match":105,"seq_b_single":0}
ccagagtacgcccattgagtctctgcacctatccttttttttctgtttatgaatctttctttgttttcggaaagccggatttggctcaggattgcccgcgtactctgg
+
U^^ac^cc_dcLdeedddbeceebeeebeeeeddb_dbeeddaddeefedecceddeeddd_ddedddbdccc^aEEEEEEEEEEEEEEEEELEELEEEEEEEEEEEL
@HELIUM_000100422_612GNAAXX:7:1:8703:2975#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5484,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacacgacgctcttccgatctcctctgaggggtcgccccaaccgaatcagtgtctaaattaataggtgtgcgataaagctctatagggtcttcttgcctcagaggagatcggaggagcgcttcagcaga
+
#############C3CAAA6@BA<aR`]eebece^cdeeeeeeebeeeedeefedeffdeeeeeefbdeeeece]Ua]abc_eebceb_`a\^^_c`a_]YaEEEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8623:20299#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":75,"pairing_fast_overlap":89,"pairing_fast_score":0.872,"pairing_mismatches":{"(G:02)->(T:34)":105,"(G:34)->(T:02)":22,"(T:02)->(A:34)":108},"score":3913,"score_norm":0.955,"seq_a_single":19,"seq_ab_match":85,"seq_b_single":19}
ccaacacacatttcaggtcaaggtgcagcttgtttctaagtggaatgatggattacattgatatttgtttatttggattgttggttttaatattggcgtgaaattggatttggttgtagtgtgttgg
+
CCCCCCC?CCCCC@CCCCCEELEEEEEEc[cd^UaY^Zaaa_ZY\ZYZccXZcd[`cab^`bbde_dcedccb]]ZY[]]\Y^[\dddcLEEEEEEEEDEDEEFLEELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9336:12626#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agtgcaa:tggtaag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtgcaa","obimultiplex_forward_tag":"agtgcaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtaag","obimultiplex_reverse_tag":"tggtaag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1734,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCACCCCCCCCCCCCCCCCcTcdX^d__dd[ab[JV`_Y^`aa^\aCABE:AEE?EEEEEEEEEEEEEEEEEEEEELEEFECCCCCC@CCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:82:15880:4232#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":45,"pairing_fast_overlap":61,"pairing_fast_score":0.776,"pairing_mismatches":{"(A:02)->(C:32)":81,"(A:02)->(G:34)":104,"(A:09)->(T:30)":63,"(T:27)->(A:02)":48},"score":1383,"score_norm":0.934,"seq_a_single":47,"seq_ab_match":57,"seq_b_single":47}
ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgtttctaaggaggcgg
+
CCCCCCC@CCCCCACCCCCCCCCBCCCCBC@CCC?CCCCCCBAC5<8EU[[``[Ya`dLdCNBRMR^UK]^Z^^E5A?;?JA+A6EBBEBDEEEEA>EEEEBELBEB?CCCCC?@BBBBBB5CCCC?B:CBBCCCCCCCCC@CCCCCCCCCC>BC
@HELIUM_000100422_612GNAAXX:7:1:8676:18144#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5573,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccagcagatcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccctttttttctcaaaacacggatttggctcaggattgccccgatctgctgg
+
CCCCCCEEDa]K\]S_^d]c_c__W^`^\QRRRQ]KXa_e]cceaaaaUc_bc\beeeaaefdeddacead````aebeeeeePZcbdc]eee^cEEEEEDEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8683:3179#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5645,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccatgtcagcggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgctgacatgg
+
CCCCCC^eZebbce[edb[ebeeeceeedeeecbeeecdeeddeeeee_dedeeeeee_aeed]f_eeecccdad_eeeecMcdbEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9367:7185#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aacggaa:aacggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacggaa","obimultiplex_forward_tag":"aacggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacggaa","obimultiplex_reverse_tag":"aacggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(g:02)->(t:34)":81,"(t:24)->(c:12)":38},"score":1414,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
C8CC8CCCA2CCAAA=AACCLAEddcW[eb`bUMABV;CKQ?5MKLLJFNAL_X_CC0>;9>DADCDEBEEECEE?ECEELCC@@CCCCA@CCCC>C;BB
@HELIUM_000100422_612GNAAXX:7:83:17312:7528#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(G:31)->(T:15)":52},"score":3539,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgcagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgcttctagttaattgg
+
CCCCCDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCEDEdXAIWdeede`][U_c`^a_bede`e`_a]eeeeeeeebcbeeeeeebXea`aDEEEEECCCCCBCCCCCCCCCCCCCCCCCCCCCCCCCDCCCCCCCCCDCCCC
@HELIUM_000100422_612GNAAXX:7:1:8940:3976#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"score":4250,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
agag
+
fedd
@HELIUM_000100422_612GNAAXX:7:1:8674:5172#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":6157,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccacaacaattagataccccactatgcttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccttgttgtggagatcggaagagcggttc
+
########A9A=A*8>CCceeebcc_c]__[a[_eeeebe`ecdeceedeeedeeeeeeeeeeeeecdddddddd[dd]acaa_a\_c\\^^Y[ZY[[cccc_[[]]NA#################
@HELIUM_000100422_612GNAAXX:7:1:8637:5000#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(C:34)->(A:08)":12},"score":6172,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccatgacgctcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccgagcgtcatgg
+
CCCCCCaUc]YFYaa____dbddc^bcccdeeeeeebebebededeeefeeddefddeeeeeeebdeedeeddee\Uaa`ZXYOY`ac___aa__\cEDEEEFEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9400:17468#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1721,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
C@CCCCCCDCCBCCBCC?AdLda`^dYZeeeea]P^WaF`Y]`]_X_A;>>0@E7EECDEBBEEEEECC?=CEBECJ@ACFA;<AA==CBCCC>CCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8697:9443#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":89,"pairing_fast_score":0.767,"pairing_mismatches":{"(A:09)->(T:34)":82,"(G:02)->(T:34)":105,"(G:25)->(A:06)":50,"(T:02)->(A:35)":103,"(T:02)->(G:34)":107,"(T:14)->(A:34)":84,"(T:26)->(A:34)":90},"score":2891,"score_norm":0.921,"seq_a_single":19,"seq_ab_match":82,"seq_b_single":19}
ccttatgtgatttcaggtcaaggtgcagcttgtttctaagtggaatgatggattacattgtcatttggttatttggattattaattttaatattgatatgaaattggatttggttgtacacataagg
+
CCCCCCCCCCCCC?CCCCBEEEEEEEEEEFbd\^]Y\^aY^YOOOHEHG>OOK]V\X`cbQNRQR\YPZS]ZZPZYZVV]PFQEOddcdC_[aEEEEEEEEEMELELECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9116:15574#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tatcagc:tatcagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatcagc","obimultiplex_forward_tag":"tatcagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatcagc","obimultiplex_reverse_tag":"tatcagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(g:02)->(t:34)":81},"score":298,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCBCCCCCCCBC=CEEEL>EEEEEEEEEDD>EE=<A=;<:>>9<<7>Z\XI_<CCBBEEEDEEDEEEEEEEEBEELCCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8693:2601#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":105,"pairing_fast_score":0.882,"pairing_mismatches":{"(C:33)->(T:02)":34,"(C:34)->(A:02)":28,"(C:34)->(T:10)":42},"score":3330,"score_norm":0.971,"seq_a_single":3,"seq_ab_match":102,"seq_b_single":3}
tctccactgagtgcgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatgggcacacatggaga
+
###EEEEEEEEEEEEEEEEEEEEEEEELEEDCEKEEEEEaaFYVb`ee`]`b_ea\_\c]`_ec]_N]eaceecbRO]W][MY\Z\^]cc^bEEEEEEEEEEEDEEEE###
@HELIUM_000100422_612GNAAXX:7:1:8661:20294#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":101,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:22)->(G:02)":11,"(C:02)->(G:33)":91,"(C:20)->(A:02)":12,"(T:02)->(C:33)":107,"(T:32)->(G:02)":30},"score":2380,"score_norm":0.95,"seq_a_single":7,"seq_ab_match":96,"seq_b_single":7}
ccagctgagcaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttttttttctcaaaacacggatttggctcaggattgccctgctcagctgg
+
>@8=>AA?C:@>::9@:ACA?B0?BCCCBJCCC]_bbbbb^]]b^^_Z_YX_YZ^TP\WSIaaZZZ]Z]bbDDDDDAA?DDD@DDDD@DDKDDDDAAA8@D@DADDKDBAB@BBB
@HELIUM_000100422_612GNAAXX:7:1:8635:3189#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(G:34)":102},"score":5263,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccagagacagcgggcaatcctgagccaaatcttttttgttttgttttgaaaaactgattaatcggacaagaataaaaaaggataggtgcagagactcaatgggcgtctctgg
+
CCCCacb_Y]cc___T_^[^\\ecbe`edddbdddcbcacccdedddddeeeeebdceee`^^Zcaa_`b^bccccc]]cEEEFEEEEEEEEEEEEEEEEELEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8754:4334#0/1_sub[28..133] {"ali_dir":"left","ali_length":56,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtattc:tgtattc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtattc","obimultiplex_forward_tag":"tgtattc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtattc","obimultiplex_reverse_tag":"tgtattc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":40,"pairing_fast_overlap":56,"pairing_fast_score":0.755,"pairing_mismatches":{"(a:34)->(t:02)":34,"(c:32)->(g:02)":48,"(c:34)->(a:02)":30},"score":561,"score_norm":0.929,"seq_a_single":52,"seq_ab_match":52,"seq_b_single":52}
ttagccctaaacgtaaactgcaaactattccataataaaataattcgcccaagaactactagcaacagcttaaaactcaaaggacttggtggtgctttctacccct
+
C@CCBCCCCACCC5A?@;A@@A>?CEBLELBDELEEE?:=9A.5=:9J669@?B@DB@_bR_\YXZ]R@<D@BEEA<EEAECCCCCBCCCCC@<CCCBC?BCC@CC
@HELIUM_000100422_612GNAAXX:7:1:9232:19869#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcactt:aacctga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcactt","obimultiplex_forward_tag":"ttcactt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacctga","obimultiplex_reverse_tag":"aacctga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":2636,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
CCCCCCCCCCDCCCBCC?CEAEEEEEEEDDD<D?CCCCE?cMX\[[[YQYZUcecZeeeceXbc]cec`beIecc`^_adcCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8661:21178#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":89,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:28)->(G:02)":90,"(A:33)->(G:02)":92,"(A:34)->(C:02)":35,"(A:34)->(N:02)":31,"(G:34)->(N:02)":32,"(G:34)->(T:02)":63,"(T:26)->(G:02)":82,"(T:31)->(N:02)":30},"score":244,"score_norm":0.91,"seq_a_single":19,"seq_ab_match":81,"seq_b_single":19}
cacgacgcgcttccattctcctaccgtgttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccacggtaggagatcggaagagcggttca
+
###################EEEEEEEEEEILLDELEEEEEEEEEEEECEEDEEEEEEEEEEELAEEEEE=EEEEEDDEE?@DEA7<2>7FDKA=/0/229<7=TM^^^A>7'78A?@@@A)>>0?;:
@HELIUM_000100422_612GNAAXX:7:1:8749:20502#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gatgacg:gatgacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatgacg","obimultiplex_forward_tag":"gatgacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatgacg","obimultiplex_reverse_tag":"gatgacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{"(g:02)->(t:34)":81,"(t:33)->(c:02)":38},"score":409,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CBBCCCCCCCA6CCCCCCBDE?EEEEEB@BB?@BBDEK)C@@.>=\_WXYI]CECEE=@=;AC+C?CA8EEEA=A@AECEL3BBAB?CCCCCCBAACCBC
@HELIUM_000100422_612GNAAXX:7:1:8693:18006#0/1 {"ali_dir":"right","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":91,"pairing_fast_score":1,"score":4520,"score_norm":1,"seq_a_single":17,"seq_ab_match":91,"seq_b_single":17}
acgacgctttccgagctcccacgtcagtgggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatggacagatcggaagagcgctt
+
#################Ebe_eb]bZZaRKM]NRRJRM[\bab_eeeacefeebedebe^daeeedeedebe_ccc`efe_ceeedeEEDEEEEFEEEEEEEEEEEEE#################
@HELIUM_000100422_612GNAAXX:7:1:8648:18692#0/1 {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"score":4252,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
ggcggacaccgagatctacactctttccctacacgacgctcttccgatctcgccggcgcaccaactctttcattcagacgtatacgtatgtatatacggggtacgtggagatcggaagagcggttcagcaggaatgccgagacagatatcgtatgcgg
+
######################?92A>B?1<A9:;87'8?<;?8;3,;CCefbebeeedWcdcefeedeeeedeeeedeeeeeeeeeeeeeeeeeefeeeebfbeeeeCCA3;A:A;?AA??>?##################################
@HELIUM_000100422_612GNAAXX:7:1:8651:11257#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":102,"pairing_fast_score":0.899,"pairing_mismatches":{"(A:02)->(G:34)":96,"(C:02)->(T:34)":106},"score":5262,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagatcgacgccattgagtctctgcacctatccttttccgttgtattctagttcgagaacccccttgttttctcaaaacatggatttggctcaggattgccccgtcgatctgg
+
CCCCCCEEDEEEEEEaNc]__cacaeebeee`deaeaeeeceeebededdbdecbefecdeebedddedeedbed^cdcdWRXUX^V\]V`cEEELEELEEEEEELEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8681:15158#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":103,"pairing_fast_score":0.95,"pairing_mismatches":{"(A:34)->(G:08)":31,"(T:02)->(G:34)":108},"score":5860,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
catctccagcgagtcccattgagtctctgcacctatcctttttttcgttttctgaatctttgtttggaaaaaaacaggatttggctcaggattgcccgtacgcgctggagatc
+
#####EEEccVb\SQ]ZQce_eec]eeePaF^\cc_]Y_eaeeeadeedebeeb`dd`decee^b`\dddeeebbbbceedV^La`[\\V^]^X^_ccEEEEEEEEEL#####
@HELIUM_000100422_612GNAAXX:7:1:8616:12437#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":100,"pairing_fast_score":0.722,"pairing_mismatches":{"(A:02)->(C:34)":105,"(A:02)->(G:34)":98,"(C:02)->(A:33)":99,"(C:02)->(A:34)":106,"(C:02)->(G:34)":92,"(C:02)->(T:34)":101,"(C:20)->(A:32)":51,"(C:34)->(A:09)":39,"(G:34)->(T:08)":44},"score":2792,"score_norm":0.91,"seq_a_single":8,"seq_ab_match":91,"seq_b_single":8}
ccagctcgtctccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccccttttttttctcaaaacacggatttggctcaggattgcccagacgagctgg
+
CCCCCCCCEEEEEEEAEEEEEAEEEEEcc^`aU[]^YXFWSU`F[[cRa\BK\]N]_aEcVQ^_J_[T__bc_[[`[U]\?D=EEEDEEEBLEEEEELKELEEELLDECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8643:10779#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":103,"pairing_fast_score":0.95,"pairing_mismatches":{"(A:02)->(G:34)":95,"(G:34)->(T:02)":6},"score":4681,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
ccagtgcacacccattgagtctctgcacctatccttttcctttggattctagttcgagaaccccttgttttctcaaaacacggatttggctcaggattgcccgtgtgcactgg
+
CCCCCLEbefeeedb_``aeeee]ccb`eebceecbeecebbb_^cacc[^bce^beeeeeeb[dcebee_]dEEEEEEEEEEEEEEEEEEEEELEEEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8653:20179#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(G:34)":100},"score":5579,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ctctgcacgggcaatcctgagccaaatccgcattttttgagaacaaaggggttctcgaactagaatacaaaggaaaacgataggtgcagagactcaatgggtgcagagtgg
+
CCCFEEEEdZdbcbf^d_]^[caa__Maa_acYY``e]e`edbcdc_eeeefede\Z_c_efccecbbbc`d\ddeec_]^\\^^[YYY]aEEEEEEEELEEEEDEFEBCC
@HELIUM_000100422_612GNAAXX:7:1:8619:7517#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":104,"pairing_fast_overlap":107,"pairing_fast_score":1,"score":5708,"score_norm":1,"seq_a_single":1,"seq_ab_match":107,"seq_b_single":1}
cccagactgtcccattgagtctctgcacctatcccttttttctcgctttctgaactctggtttgttcgcgtaaaccaggatttggctcaggattgcccgacagtctggg
+
CbZebeebbeedebeaaabeceeeeebebdc]ced_deeeeebbeddeecfe_eedeee^edeeedeeef^cd`ccecaaEEEEEEEEEEEEEEDEEEEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8637:18990#0/1 {"ali_dir":"right","ali_length":67,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":60,"pairing_fast_overlap":67,"pairing_fast_score":0.938,"pairing_mismatches":{"(T:10)->(C:33)":100},"score":4272,"score_norm":0.985,"seq_a_single":41,"seq_ab_match":66,"seq_b_single":41}
cgagatcctcgctcgttccctacacgacgctcttccggtctacgagcaccattgagtctctgcacctatctctgtgtgccttggctcaggattgccctgctcgtgtggagatcgcaggagcggttcagcaggagtgcggacaccgacat
+
##################@C:@C:A;9?==5=55(2((?@<[^^^^^Ubeeee_bceebeebc\[cbcTcacDcc`c]_Kea_V^__a_Uaa]_a_^`_Eaa``Y`ebCCC######################################
@HELIUM_000100422_612GNAAXX:7:1:8699:6281#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":100,"pairing_fast_score":0.948,"pairing_mismatches":{"(G:02)->(T:34)":101,"(T:02)->(G:34)":108},"score":4514,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
tccgatctccagagtagcggggcaatcctgagccaaatcttgtattattcggatgaatttcgggcgatgaggcgagataggtgcagagactcaatggcgctactctggagatcgga
+
;AC?3?)2K]eXcaWdccP`]aaSSXWX_Y_b[XTTOKd^edeeeeeePaU_Yab`X]U_`bXZdeSSZ[]Z[_[cZ_c___bEDEEEEEEEEBEEEDEELEDEAEEL########
@HELIUM_000100422_612GNAAXX:7:1:8635:8208#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(T:34)":99},"score":5992,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagagacagcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctcctggttttctcaaaacacggatttggctcaggattgcccgctgtctctgg
+
CCCC@CW__\W\c^Z`]Ta^U^ZZJc_L`]^^]Y_LaaZfbcc^_ed_eedeeeeeeeeeeeee]eedeceeedebadcdeeebTeeeeWeeZRcL`aLEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:59:4117:16319#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":61,"pairing_fast_score":0.897,"pairing_mismatches":{"(A:02)->(G:34)":105,"(T:34)->(G:02)":49},"score":1297,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ccgaagtagttagatacccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagcctgttctactacttcgg
+
CCCCCCCCCCCCCCCCCCCCCCBBCCCCCCCCCCCCCCCCCCCCC=CELEbb_dZXeadecb[__\]]NREEEEEEDCCDBEEED=CEDBEEEEDEEEEEEEEELEEECCCCCCCCCCCCCBBCCCCCCCCCCCCCBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8690:20160#0/1 {"ali_dir":"right","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":79,"pairing_fast_score":0.776,"pairing_mismatches":{"(A:02)->(C:34)":104,"(A:02)->(G:34)":97,"(C:33)->(A:02)":30,"(G:02)->(A:34)":85,"(G:29)->(A:02)":42},"score":1696,"score_norm":0.937,"seq_a_single":29,"seq_ab_match":74,"seq_b_single":29}
tctatccctacaagacgctcctccgatctccacgcgcgatgggcaatcctgagccaaatcttgtttctacgacgagataggtgcagagactcaatggatcgcgcatggagatcggaggagcggtcagaagcaatgcg
+
#############################KDDDDDDDA@DDG`^Y`^\_YddYdYZdb^`daa_ccZcE<:EEE@EEEECDEEELEEEEEEEEEEELEDDEEELDEEE#############################
@HELIUM_000100422_612GNAAXX:7:1:8676:7870#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":87,"pairing_fast_score":0.905,"pairing_mismatches":{"(A:02)->(G:34)":99,"(T:02)->(G:34)":105},"score":3886,"score_norm":0.977,"seq_a_single":21,"seq_ab_match":85,"seq_b_single":21}
ccagagcagtcccattgagtctctgcacctatcctttatgtttatttttgctttctgaagcccttttgttttgtttgttttttctgaaaacaaaaaaggatttggctcaggattgcccgactgctctgg
+
CCCCCCCCCCCCCBCCCCCCCEEEEEEEEc_cLddedefbd]decedd^bbee```Yc\X\b_bbdeddcddcaa\cb`d`dddaDDDDDEEEEEEEELEEEEELEEECCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:99:18434:16307#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtctcct:gcctcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtctcct","obimultiplex_forward_tag":"gtctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":58,"pairing_fast_score":0.927,"pairing_mismatches":{},"score":4007,"score_norm":0.983,"seq_a_single":50,"seq_ab_match":57,"seq_b_single":50}
tttt
+
bdee
@HELIUM_000100422_612GNAAXX:7:1:9322:20353#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acaatgg:acaatgg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acaatgg","obimultiplex_forward_tag":"acaatgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaatgg","obimultiplex_reverse_tag":"acaatgg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(G:34)":56},"score":1109,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCBC?CcMeceeebMb[X^[PLSab+EEEEEEEE<2;;<BE9LE=EEDEEEEEEEEEEEEEEELBECHCCCCCCCBCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9157:8023#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgaacg:gcgaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgaacg","obimultiplex_forward_tag":"gcgaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgaacg","obimultiplex_reverse_tag":"gcgaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":2648,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC=CELEEEEEEBDEEEEDDAEEb[aEO^a\\WX[[XY`YTa_Zb`][^]aR^`daTbc\`\dbb^CCCCCCBCCBCCCBCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8671:5804#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":100,"pairing_fast_score":0.928,"pairing_mismatches":{"(C:02)->(A:34)":102,"(T:02)->(G:34)":105},"score":4664,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
cccacagcacagggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtgtgctgtggg
+
CCCCCCCC\[]X[\ab^^WaWcb]aeRccU__^ca_beeXdbdddedddd^deeeabeee_]bd^SYYZYc___cQ`UaaEEEEEEEEEEEEEEEEEEEEELDELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:18:1969:17784#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":61,"pairing_fast_score":0.948,"pairing_mismatches":{"(A:02)->(T:33)":106,"(T:02)->(A:34)":108},"score":787,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ccgcctccttagaacaggcatcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaggaggcgg
+
CCCCCCCCCCCCCCCCCCDCCCCCCBDCBCCCCCCCCCCBDCCCCACEEE3DCCCECBC?a_bVd^[YaY\_DD>C??5A@C@CCA?:?9EEEDDEEEEFEEEEEKELCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCDDACCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:94:14594:8342#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":61,"pairing_fast_score":0.914,"pairing_mismatches":{"(A:02)->(G:34)":104,"(G:02)->(T:34)":108},"score":1678,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ccaattaacttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagccctgttctagttaattgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCDCCCCBCCDCCCCCCCBBEEC_abbedcac^Y`a`_`Za^c\a^UaAECDEAEEB>E5BEBEEECDE=EEEEELCEELCCC@CCCCCCCCCACCCCCCCCCCCCCCCCCCCDCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8661:17299#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":6100,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
agtcgactggggcaatcctgagccaaatccgtgttttgagaaaacaagaaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagtcgactgg
+
CCCCU\cbeecee`e]eebbbeedcee`cfccc[]c_bdadceee^bdbdeeededcecbcdeae`ebebacdScbb`c_\^[[QZ[UU^_caEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8610:14734#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":106,"pairing_fast_score":0.99,"pairing_mismatches":{"(A:02)->(G:34)":108},"score":6730,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccacgcagacaccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgccctgtctgcgtggag
+
?:J^^ab\bUbe\abeee]beee`\_\ca^cd_eeee_eeeeeceeeeedeebebdeedcdadeeddddaeeebebca^cc`e_UbcXYYZXW]L\Xc_\\aEFEEEL##
@HELIUM_000100422_612GNAAXX:7:1:9193:16574#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgacac:gcgacac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgacac","obimultiplex_forward_tag":"gcgacac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgacac","obimultiplex_reverse_tag":"gcgacac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(a:02)->(g:31)":76,"(a:34)->(g:02)":30,"(c:02)->(a:32)":73,"(c:34)->(a:02)":41},"score":1112,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
C@CCCCCCCCCCCCCCCCELE4EEEEEEELEEEEEEBB>EL?E/1,/2?CC=8\KY\U`cc_`\URaVZ`^aJE?IADACECCCCCCC@CCAC@CCBC@
@HELIUM_000100422_612GNAAXX:7:1:8714:6963#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:ttctaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttctaac","obimultiplex_reverse_tag":"ttctaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":61,"pairing_fast_score":0.931,"pairing_mismatches":{},"score":3303,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
cctctaa
+
`^^`\US
@HELIUM_000100422_612GNAAXX:7:1:8645:9506#0/1 {"ali_dir":"right","ali_length":54,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":51,"pairing_fast_overlap":54,"pairing_fast_score":1,"score":3972,"score_norm":1,"seq_a_single":54,"seq_ab_match":54,"seq_b_single":54}
tataccgcgaccaccgagatctacactctttccctcacgacgctcttccgatctcccagagcgatccgaaggcgtcaaggaacactctggggactctcggcaacggatagatcggaagagcgttcagcaggaatgccgagaccgatatcgtatgccgtcttc
+
######?=725A9/::=:67=44?374;6<1?:A)@B@:BC@?BABCCCAABA@eeeeddbea`eeeecefedeceffeeefedeeeeeeeedecfefeeeeeeddde?CCC=CCCCCCAACCA9A===<@?@3<<8?>A=>+A5-*571/9)5?)?>>A6>
@HELIUM_000100422_612GNAAXX:7:1:8851:3693#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(a:34)->(t:06)":35,"(c:02)->(g:34)":76},"score":2702,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CBCCCCCCCCCCCCCCCDLEEEa^\ZVc`cbRPPGQY^]^OQPORPOMNPR]bYW_[_`T\U`___ab_c__cEDLDEDECCCDCCBACCCCCCBC?CC
@HELIUM_000100422_612GNAAXX:7:1:8685:11547#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5489,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacgatcgcgccattgagtctctgcacctatcctttttgattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgcccactgtgcgtgg
+
CCCEEEEEc_cTVZTX]e]ebcbc_c___\`c[cedccc^bRdecefdeddeeeU_dacccdbdYdfd_a``ccdecc]`c`cb`cRbdEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8643:16566#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":103,"pairing_fast_score":0.95,"pairing_mismatches":{"(C:02)->(T:34)":108,"(T:02)->(G:34)":103},"score":3833,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
ccagtcacgaggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggctcgtactgg
+
CCCCCX_YaRWceRRRQMU]L\WNP]\Z`XQ_adaccc]ee`beeeeeddeeeedceebdeeaZb^aEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEELEEEELCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8633:14783#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":4264,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
gcgacgctcctccgatctcctgtaggtttagataccccactatgcttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccactcgaaggagatcggaagagcggttc
+
##################EEEEEaTa_ce_ebe_eeeeb`\a[aebecfeeecdeefeeeeeeddedddbddddd_dd[[_ccEEEEEEEEEEEEEEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8786:4346#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcactt:ttcactt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcactt","obimultiplex_forward_tag":"ttcactt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcactt","obimultiplex_reverse_tag":"ttcactt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(G:23)":50},"score":1934,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtagtaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCDCCCDBC^_bb_ddeeZddbd_c_d^__]baaac_C6AC?EEDEECEEEEEEEEEEEEEEEEEEKEEEECCCCCCACCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:9319:1746#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gccgtgc:gccgtgc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccgtgc","obimultiplex_forward_tag":"gccgtgc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccgtgc","obimultiplex_reverse_tag":"gccgtgc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":63,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(G:23)":47,"(A:09)->(C:02)":42},"score":207,"score_norm":0.905,"seq_a_single":45,"seq_ab_match":57,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgcaagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
C@C;AA?ACDAC;C?CCCE>EEE@<DB@>@3?1;D:::;=>3YWXaA6@:2?>BEBCEE@:BEEEEEDABEEELELFKCLEBC?CC?CCCCCCC@CCCC
@HELIUM_000100422_612GNAAXX:7:1:9191:13000#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tggccac:tggccac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggccac","obimultiplex_forward_tag":"tggccac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggccac","obimultiplex_reverse_tag":"tggccac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{},"score":1421,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCC3????CCCCCBBCBA_b\_dba_V^QR\GNJMJP\W[KZ]aEE>=7A:EEE-EDEEE=EELEEEDEEEEEEEK@EELECAACA?CCCCCCC?CCCC
@HELIUM_000100422_612GNAAXX:7:1:8691:15712#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":102,"pairing_fast_score":0.879,"pairing_mismatches":{"(T:02)->(C:33)":102,"(T:34)->(G:08)":42,"(T:34)->(G:13)":15},"score":4533,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagctgagcaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccctgctcagctgg
+
BCCCCCUa_\cMccEVYbbQQQRJJQPRQ_bcb`^XZ\NaYF][eZcceb__c_bea`Zbeedbba_d\dc`_cecbeceadcEEEEEEEEEEEEE?EEEBKEEDEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8629:2603#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":73,"pairing_fast_score":0.886,"pairing_mismatches":{"(T:02)->(C:34)":95,"(T:34)->(G:02)":51},"score":1499,"score_norm":0.973,"seq_a_single":35,"seq_ab_match":71,"seq_b_single":35}
ccacgcacagtgcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatcagctagcgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCBCCDDDEEEDEEEEEEDELCDBDac]SV]LMX^RaaTc`aZ]^`dbb?EEEEEFEEEEEE=CLEEEEEEEAEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8612:13430#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":85,"pairing_fast_score":0.854,"pairing_mismatches":{"(A:09)->(C:34)":96,"(A:33)->(G:02)":27,"(G:34)->(T:07)":61},"score":4667,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
ccagcacatgcccgaaggcgtcaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacacgactctcggcaacggatatcctgtgtgctgg
+
CCCCCCCCCCCCCCCCCCCCBCCEEEKce[eacadc[[Y[M^eVedaWUZ\UJ[`GJMNIGY`\]\]NdeeeeYeeeecbb_^^bcZ]ccO]VT[FXZSUYZ^[a`U[CCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:30:8575:17507#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{"(C:02)->(T:34)":107},"score":1900,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagccctgttctagatattcgg
+
CCCCCCCCCCCCCBCCCCCCCCCCCCCCCCBCCCCCCCCCCCCCCC__[cc^dbbece_acNHZcb]^cb_c_]b?EBDEECEEEE@EEEEFEEBEEEEEEEEBEELECCCCDCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9263:6234#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tggccac:tggccac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggccac","obimultiplex_forward_tag":"tggccac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggccac","obimultiplex_reverse_tag":"tggccac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"score":1849,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
C?DBCCCCBCCCCCBBCBX\c^deecec__eedcc][\\c___]cEEEEEE=EEEEEEEDEEEEEEEEEEEEEEELFEEDECCCCCCCCCCCCD;CCCC
@HELIUM_000100422_612GNAAXX:7:1:8650:14549#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":105,"pairing_fast_overlap":108,"pairing_fast_score":1,"score":5110,"score_norm":1,"seq_a_single":0,"seq_ab_match":108,"seq_b_single":0}
ctaacgctgggcaatcctgagccaaatcacgttttccgaaaacaaacgaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggagcgtacgtgg
+
EEEEEaU^[_c]a``Sc]Xe[[eccbbcacYa_babeb_deeeeddddfeeebedceccea_bbcdbec__d_`d`a_aEEEEEEEEEEEEEEEDEEEEEEEEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:8697:1513#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(G:34)->(T:06)":31},"score":5689,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccagatctattagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctagatctggagatagaaagagcggttc
+
6664399?=/=2=;:=A8^eece\ZZYMQQGORZ_caede]eedede]_eeeefeeeeeeee_eeeeeeeedceeeeeddcedbc^cedeeb^ccbeceebdEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8635:15455#0/1 {"ali_dir":"right","ali_length":69,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":69,"pairing_fast_score":1,"score":4669,"score_norm":1,"seq_a_single":39,"seq_ab_match":69,"seq_b_single":39}
agatctacgctccttccccacacgacgctcttccgatctccattgagtctctgcacctatccaccaaacatccggcggacttggctcaggattgccccgctactctggagatcggaagagcggttcagcaggaatgccgagacagat
+
###############################@A>2.5-?WbZbebeeedac_a]W^^Pcccdbee]eee`ceedcedeeeebeeddb_eeeecc_c[WcV\^L^c]_c=CC;8C?CACC7';:788986:?################
@HELIUM_000100422_612GNAAXX:7:1:8618:8140#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":94},"score":5529,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccacgtcagtcccattgagtctctgcacctatcttttttttcttttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccccgtacacgtgg
+
CCCC\^\Y^^YUY\W[YUceeec_Zcc][]WWdXbbdcccdddddd`cbeeeeee_ca^beedbddddfcddbbecdc^_X[VZ`ccEEEEEELEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8915:9719#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgaacg:gcgaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgaacg","obimultiplex_forward_tag":"gcgaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgaacg","obimultiplex_reverse_tag":"gcgaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":63,"pairing_fast_score":0.783,"pairing_mismatches":{"(c:02)->(t:34)":64,"(c:28)->(a:02)":41},"score":296,"score_norm":0.937,"seq_a_single":45,"seq_ab_match":59,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CBCCCCCCCCCCCCCCCCEELEEDEEDDEEEEB=C==-=?FA?EEECEC=?@CA?:??_QWZ`LBEE=EEEEBEDEDDELBCBCCC@BCCCCCC9CCCC
@HELIUM_000100422_612GNAAXX:7:1:8674:5645#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5966,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccgatctccggtgagaggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgtctcaccggagatcggaagagcgtttcagaag
+
861C@AC@?C=;CC8A;C@C?BCUeeeede]eedcdda\cd`ceebeeffeedefeeccgeeedcfeecebceecceeeee]feccce\a_`aa_caaebeb`YYRZT?<?####################
@HELIUM_000100422_612GNAAXX:7:1:8658:7682#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":89,"pairing_fast_score":0.733,"pairing_mismatches":{"(A:02)->(T:34)":82,"(G:02)->(T:34)":105,"(G:34)->(A:02)":22,"(T:02)->(A:34)":103,"(T:02)->(G:34)":107,"(T:33)->(G:02)":30},"score":749,"score_norm":0.91,"seq_a_single":19,"seq_ab_match":81,"seq_b_single":19}
ccttatgtgatttcaggtcaaggtgcagcttgtttctaagtggaatgatggattacattgtcatttggttatttggattattaattttaatattgatatgaaattggatttggttgtacacataagg
+
CCCDCCCCCCCCCCCCCCBEELEEEEEEEKEEEEEEDEEDEEEBD????@D:CCBBBD`cXX\_acd]ccEEEEEEEDEEELELEEEEELEEEEEEEEEEEELELELECCCCCCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:31:8254:11099#0/1_sub[29..128] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaagtag:aagtagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":1,"obimultiplex_reverse_match":"aagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aagtagt","obimultiplex_reverse_tag":"aagtagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":61,"pairing_fast_score":0.914,"pairing_mismatches":{"(c:02)->(a:33)":75},"score":2852,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCACCLEEa`a_X[^W]ZPIQRGS^\ZV[\[ZSVVXVY`dWeb]\`VQ_\cc`^d^]bDKCDCCCCCCCCCC;CCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:7492#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:34)->(G:18)":34},"score":4904,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccacagtcgagccattgagtctctgcacctatcttttttttcttttcgctttctgaacctttgtttgttttcggaaaacgcgatttggctcaggattgcccgcacacatggg
+
CCCCEEEEEEEEEFEEEdcacc_Y_cZ\Y\[[eDddbcccddddddcdacdbdbe^bc^deedcddddcdedbceeecce__\`accScEEEEEEEEEEEEEEEEEEFCCCC
@HELIUM_000100422_612GNAAXX:7:1:8612:14001#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4101,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatccacactctttccctacacgacgctcttccgatctcctaatcatcaccaactctttcattcagacgtatacgtatgtatatacggtctgcatggagatcggaagagcggttcagcaggaatgccgagacggatatcgtatgcg
+
############################@7ACC5?CACAB:B?A3AAC?cca_aM^\XdcddcceeebeedeeeaeeeeeeeefdebeeebeeeedeeeY^][Y_a__AA==6=6AAA#######################################
@HELIUM_000100422_612GNAAXX:7:1:9245:8319#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgaacg:gcgaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgaacg","obimultiplex_forward_tag":"gcgaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgaacg","obimultiplex_reverse_tag":"gcgaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(G:14)":58,"(A:26)->(G:08)":49,"(G:02)->(T:11)":55},"score":429,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactacctgcgatagcttaaaactcaaaggacttggcggtgctttataccctt
+
?1C7=A=A?CCCACCBB?CEEEBDEDEB;D@2?5:/@81<:>8^a]^E>NKAV55=<8CDD;D<@DDA?>??C>CCCCC*JA<@@BB=BB?BA65??B:B
@HELIUM_000100422_612GNAAXX:7:1:8676:8560#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":85,"pairing_fast_score":0.646,"pairing_mismatches":{"(A:34)->(C:02)":29,"(A:34)->(G:02)":62,"(C:33)->(G:02)":39,"(C:34)->(A:02)":28,"(C:34)->(G:02)":51,"(G:32)->(C:02)":44,"(G:34)->(A:02)":31,"(T:34)->(A:02)":33,"(T:34)->(G:02)":59},"score":2014,"score_norm":0.859,"seq_a_single":23,"seq_ab_match":73,"seq_b_single":23}
ccagagagtcgggatgacgccaaggaacacgataaaagctaaggcaccggcgagcctttcgagggctccgtcggctgccgaggccaacagaaaaaaatatgactctcggcaacggatatcgatgcgtctgg
+
CCCCCCCCCCCCCCCCCCCCCCCEEELLLLLLLEEEDEKEEEEJEEEEEALEEEE?EBLDELECEEEE>DZR\O\SXXYYc[`eeeZda_KYPSRZ\OZW\b^bWadaCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8613:3975#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":89,"pairing_fast_score":0.953,"pairing_mismatches":{"(G:34)->(A:07)":29},"score":4196,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
cctggcttgattcggttggggcgaccaaggacatctcatcctataaatacaagattaattaatcaacacacagaccctacaaatagactaaaaacataagctaccttagggataacagcaagccagg
+
DCCCCCCCCCCCCCCCCCCada]aX\`SGJQRXR][_ea`edeeeeec`eeeedaeccaeccc^aeceddd_]c`Ua[]^^^aaEEEEBBEEEEDEEEECEEDEEEEECCCCBCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:34:9208:5914#0/1_sub[29..128] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:cctcctt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":1,"obimultiplex_reverse_match":"aagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"cctcctt","obimultiplex_reverse_tag":"cctcctt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":2881,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCDCCCCCCCEEEEEEEEDEEFEDDEEDCE`Q_ca_cbbbebeeebdedebb\deeeedeaafeedTbee]CCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8643:4846#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":102,"pairing_fast_score":0.939,"pairing_mismatches":{"(C:02)->(G:08)":107,"(T:10)->(C:33)":65},"score":5099,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccatgcagtgcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgcagtgcatgg
+
CDCCCCEEEEEEEDEEEEEa^\a\Nbead`eeeec``d]dbc^dbZ[edeZbeeadd_feebecEc[dbcbcdeT_cd_decbb^eede__aZUaEEEEEEEBEEE2ECCBBCC
@HELIUM_000100422_612GNAAXX:7:1:8624:18805#0/1 {"ali_dir":"right","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":59,"pairing_fast_overlap":74,"pairing_fast_score":0.831,"pairing_mismatches":{"(A:34)->(C:02)":61,"(A:34)->(G:02)":41,"(G:34)->(C:02)":45},"score":1757,"score_norm":0.959,"seq_a_single":34,"seq_ab_match":71,"seq_b_single":34}
ttgccttcttcccctgcccacccgcttcccatccccacacacacgccattgagtctctgcacctatccacacacacgccattggctcaggattgccccgtgtgtgtggagatcggaagagcggttcacaggaatgccgagac
+
##################################FFEEEELEDELEE=EEEEEEEEEEEELEEEEEEEE`Ya^d[]]\d\ZScS\]^L]I[`X\Y\]NaADDDCDDCB##################################
@HELIUM_000100422_612GNAAXX:7:1:8667:13460#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":102,"pairing_fast_score":0.788,"pairing_mismatches":{"(A:02)->(C:17)":64,"(A:02)->(G:32)":96,"(A:26)->(T:12)":60,"(C:02)->(T:31)":108,"(C:33)->(G:02)":12,"(G:02)->(C:17)":104},"score":580,"score_norm":0.941,"seq_a_single":6,"seq_ab_match":96,"seq_b_single":6}
ccacgcatgcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccccgcatgcgtgg
+
BBBB<AD@>@CKC@CCCDCCDDCDDDDDDD>=C8<@DDD@DDBB>DC@C^SKFUGSXEX=S2\;?<CC?CBAAA@ACAC5@>6=CCCCCCBCCCCJBBC@@=@;:C<IA@AAAA
@HELIUM_000100422_612GNAAXX:7:1:8635:11530#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:15)->(G:34)":88},"score":5223,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
ccagcacagctccattgagtctctgcacctatccctttttgattttaactttctgaacttttatttgttttcagaaagaaggatttggctcaggattgcccagctgtgctgg
+
CCCCEEEEEeaecdcdUaa``[ccc_c\\_Uaebcbeedd_ZdeeecWddeeedeZc_bbeedb_bddedddb``Z\]\VQRRRRaaDcEEEEEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:9208:12835#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aaccaag:aaccaag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccaag","obimultiplex_forward_tag":"aaccaag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccaag","obimultiplex_reverse_tag":"aaccaag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1702,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCELELEEEEEEEEEEDDEEEBE=EBECCECEDEEdac[bbb]\W_[W\ZV[`]\]aYaKaRORCDBCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8687:16366#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":89,"pairing_fast_score":0.779,"pairing_mismatches":{"(G:33)->(T:16)":36,"(G:34)->(T:02)":23,"(T:02)->(C:34)":99,"(T:02)->(G:34)":103},"score":2698,"score_norm":0.944,"seq_a_single":19,"seq_ab_match":84,"seq_b_single":19}
ccgaatatcctgttatccctaaggtagctttatttggtgatcttatttaagggtctttgtatgacttaatggtctttgtaataaaggatgatgtggtccttggtcgccccaaccgaatgatattcgg
+
CCCCCCCCCCCCCCCCCCCEEELECEEEEEFEEcbCceMbce`c`c``bcPdcbe_eee`d\dYZaca_cZVXZYaEEEEEEEEEEEEEEEEEEELEELEEELEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9398:19593#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttcctgg:gccggcg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcctgg","obimultiplex_forward_tag":"ttcctgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccggcg","obimultiplex_reverse_tag":"gccggcg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"score":4238,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
tttt
+
edee
@HELIUM_000100422_612GNAAXX:7:1:9345:10338#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":1956,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
tataccgcggccaccgagatctactctctttcccttcacgacgctcttccgatctccacggtacttagataccccactatgctagaggagcctgttctagtaccgtggagatcggaagcgcggttcagcaggaagccgagaccgatctcgtctgcggtctact
+
#################################A=+69AA3@4@B?CCCCCC=C7cc_cbYRVZW^dV`^X[^UY`^__\\ccbb`EEEEEEEECEEEEEEEEEEEEE#######################################################
@HELIUM_000100422_612GNAAXX:7:1:8645:17981#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":83,"pairing_fast_score":0.838,"pairing_mismatches":{"(A:07)->(C:31)":84,"(A:34)->(T:02)":28,"(G:28)->(C:02)":37,"(G:34)->(C:02)":30},"score":2805,"score_norm":0.952,"seq_a_single":25,"seq_ab_match":79,"seq_b_single":25}
tccctaccggaccctcctccgagtcccacgactctggggcaatcctgagccaaatcctgttttctcaaaacaaaggataggtgcagagactcaatggcagagtcgtggagatcggaagagcggtccagcagga
+
#########################ECLELEEEEAEFEEEEEEBEEEEbJdcZbbUeee^b\ed`^]XebZb_W_NV]^\TTXD[Z\Y\[_a``aEDE5EAEEEEEEE#########################
@HELIUM_000100422_612GNAAXX:7:1:8675:11754#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":102,"pairing_fast_score":0.899,"pairing_mismatches":{"(A:02)->(G:34)":96,"(C:02)->(T:34)":99,"(G:02)->(C:34)":102},"score":4855,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccagcactctcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccgagagtgctgg
+
CCCCCCEEEEdVecaaPccT]I^^W\[Zcea`ebS[[L\W`]edebeabceY_dedeeeecee``b^eabeb`cee^cce``_KbEEEEEEEEEELEELEELEEEEEECBCCCC
@HELIUM_000100422_612GNAAXX:7:1:8800:20899#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agtgcaa:agtgcaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtgcaa","obimultiplex_forward_tag":"agtgcaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtgcaa","obimultiplex_reverse_tag":"agtgcaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":43,"pairing_fast_overlap":63,"pairing_fast_score":0.717,"pairing_mismatches":{"(c:16)->(a:32)":66,"(c:31)->(g:02)":41},"score":482,"score_norm":0.921,"seq_a_single":45,"seq_ab_match":58,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
C>@CCCCA=?AAACCCCCLCCELEEEI?EC?E?@E?@>EEIBED*D@C?:?@?C??C:CC??bZ`B`]SO[aAEEEEDBEECCCCCCC@CCCCC<CCCC
@HELIUM_000100422_612GNAAXX:7:1:8641:2066#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":86,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:34)->(C:02)":30,"(A:34)->(T:02)":39,"(G:34)->(A:02)":25},"score":4105,"score_norm":0.965,"seq_a_single":22,"seq_ab_match":83,"seq_b_single":22}
ccacgtgtacgccgaaggcgtcaaggaacactgtgcctaacccggggacgcggctggcttgctggccgcaccctgagttgcaatgctatataatccacacgactctcggcaacggatatccgtacacgtg
+
CCCCCCCCCCCCCCCCCCCCCCEELEEEELEDEEEEEELEEEEaUc_[bccQe_]edbe\ec_edc^c^Sbaec__abLee_b^_`d``]T[JGT`VYZ[aa`b\^Y_CCCCCCCCCDCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8670:20599#0/1 {"ali_dir":"left","ali_length":66,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":66,"pairing_fast_score":0.794,"pairing_mismatches":{"(A:28)->(G:02)":71,"(A:33)->(G:02)":59,"(G:34)->(T:02)":58},"score":731,"score_norm":0.939,"seq_a_single":42,"seq_ab_match":62,"seq_b_single":42}
cccagtcagacgatatccgttgccgagagtcgttttgacatatagaatacagaatacgacgcgcaccccgaccgcgcaacgtatctcggaggcggggggggtgaatgccaattcgttaggtgttccttgacgccttcgggtctgactggg
+
CCCCCCCCCCCCCCCCCCBCCCCCCCCCCCCCCCCCCCCBCCEEEECEEECEKEEEELKDEEEAECEDE=F??A?D?BP_RISWIWYYUXWMX@*@?CECEECBEEECC?CCCCCCCCCCCCCCCCCCCCBCCBCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:10502#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(C:02)->(G:34)":102},"score":4693,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagagcgagtgggcaatcctgagccaaatctttatttttagaaaaacaagggtttaaaaactagactagaataaaaagggataggtgcagagactcaatggactcgctctgg
+
CCCCCEEEcc_dY\]^R^WWRM]bKeebec`ddd`cbdb^^_Zddebdd\daeWcdcdadebb\dc__]d[Ua^Vc[[]cEEEEEEEEEEEEEEEEEEEEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:9024:9748#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"score":2802,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacataagctattccataacaaaataattcgccagagaactactagcaacagattaaacctcaaaggacttggcggtgctttataccctt
+
CDC@CCCCCCCCDBCCBCEEEELEEEEEEEEE`V``^]]c_^ccebce\e\Oececbfeab_ec\_^c\dcadEEEEBBEECCCCCCCCCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8988:13344#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agatcta:agatcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agatcta","obimultiplex_forward_tag":"agatcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agatcta","obimultiplex_reverse_tag":"agatcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(c:34)->(a:02)":42,"(t:33)->(a:02)":35},"score":1427,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactactggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
BCCCCCCCCCCCCCCCCACELELCFEEEEEEEEDKEEEBEEL:EEEEBBB@Aa_`^c`_bWWd]c_c]^\dU`aDEEEE?DCCCCCCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:14577#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":100,"pairing_fast_score":0.876,"pairing_mismatches":{"(C:02)->(A:34)":94,"(G:34)->(T:24)":42,"(T:02)->(C:30)":102},"score":4311,"score_norm":0.97,"seq_a_single":8,"seq_ab_match":97,"seq_b_single":8}
tccgatctcccactgtcgagggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatggtcgacagtgggagatcgga
+
########EEEEEEcSZbcPcc`\ea`eaVb\^c^_cb]eeCeeeeeeededeeeeed_bceecce`ceeedbeebbcEEEEEEEEEEEEEEELEEEEEEEHEEEEEE########
@HELIUM_000100422_612GNAAXX:7:1:8668:19462#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":100,"pairing_fast_score":0.876,"pairing_mismatches":{"(A:02)->(C:34)":82,"(C:02)->(G:34)":86,"(T:02)->(C:34)":75},"score":2677,"score_norm":0.97,"seq_a_single":8,"seq_ab_match":97,"seq_b_single":8}
ccagcagatcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccctttttttttctcaaaacacggatttggctcaggattgccccgatctgctgg
+
CCCACCC@EEEEEBEEEEEEEEEEEEEEEEc`cc_c_bMcca`^[[^_cc\abZdaR]_YcceabYR]`]cEEBLCEEEEELEEELECCEEEDEEEEEEEEEEEEDC?AA;??AAA
@HELIUM_000100422_612GNAAXX:7:1:8686:6093#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":85,"pairing_fast_score":0.634,"pairing_mismatches":{"(A:02)->(C:32)":102,"(A:02)->(G:33)":108,"(C:30)->(A:02)":40,"(C:33)->(T:02)":28,"(G:02)->(A:30)":74,"(G:02)->(T:33)":98,"(G:30)->(T:02)":33,"(T:21)->(C:02)":51,"(T:30)->(C:02)":26},"score":158,"score_norm":0.882,"seq_a_single":23,"seq_ab_match":75,"seq_b_single":23}
ccgtcacgacactcttcccatcaccttccgctggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgagcggaaggggatcggagaagcgatgaagcgc
+
#######################KDHCKADD@H<5<>>AHAAA<<<<<:3?;/898=8@@8@9=XXV]>A>ADHAAA:DDDBDA>A>A99:9:>ADCKCCCJADC>DK#######################
@HELIUM_000100422_612GNAAXX:7:1:8701:12541#0/1 {"ali_dir":"right","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":87,"pairing_fast_score":1,"score":5235,"score_norm":1,"seq_a_single":21,"seq_ab_match":87,"seq_b_single":21}
cacacgacgctcttccgatctccacagcgctcgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagaagatcggaagagcggttcagc
+
########?@<A@@7=55??<deccab_cca^H^aacecceeebU_e^ac^Wcedeeeecddddeddcdddbe`dbeeea_eeeb_cc_ccaee\cEEEEEEEEEEEE#####################
@HELIUM_000100422_612GNAAXX:7:97:18490:5880#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":61,"pairing_fast_score":0.966,"pairing_mismatches":{"(T:02)->(A:34)":107},"score":3754,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
ccaattaactagaacaggctcctctagaagggtataaagcaccgccaagtcctttgagttttaagctattgccggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtgggggtatctaagttaattgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC_a_Z[^][ccedbc_c]]^bcbb\Yac__Y_[[[Z\\^ab]]aca^^_eeee_RYZUcELECCCCCCCCCCCCCCCCCCCCCCCBBCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8646:1303#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":106,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:02)->(T:34)":102},"score":5818,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccacgcatgcgccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgcccgacgtcagtggag
+
=@`]a]TX`]cZaK]cZcabaee^aa^_[R`dae_eeebebedceeeeefeeeeddeededadeeddddbeed`dccZa_`ad_^_dEEEEEEEEEEEEEELEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:9304:1860#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ggtgaga:ggtgaga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggtgaga","obimultiplex_forward_tag":"ggtgaga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggtgaga","obimultiplex_reverse_tag":"ggtgaga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3086,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC<CEEEEEEEEEccWcRPIQRKX\QN[XWXYcRc\`aae`eb\eZcb\[YZ^beaZb_d_Kc^ZSCCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9249:11549#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttatgtg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatgtg","obimultiplex_forward_tag":"ttatgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":3090,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCCCCCCCCCCCELEd\Sa\X_c``YYJUZ\Y^c]OSUWSRKUTSWX[O[ZRQPLPR`__b_`b\c^[aMaFEECCCCCCCCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:18975#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":89,"pairing_fast_score":0.605,"pairing_mismatches":{"(A:28)->(G:02)":58,"(A:33)->(G:02)":42,"(A:34)->(G:02)":49,"(C:02)->(G:34)":105,"(C:30)->(G:02)":51,"(G:31)->(C:02)":61,"(G:31)->(T:02)":50,"(G:34)->(A:02)":59,"(T:28)->(C:02)":55,"(T:31)->(G:02)":36},"score":1278,"score_norm":0.865,"seq_a_single":19,"seq_ab_match":77,"seq_b_single":19}
cgagagagggatgacgccaaggaacacggtaaagctaaggcaccggcgagcccttcgagggctccgtcggctgccgaggccaacagaaaaaaatatgactctcggcaacggatatcctctctcgtgg
+
CCCCCCCCCCCCCCCCCCCEEELEEEEEECEEEEEIELDBCKDDDEECLIH?CAFDCFLBIED=ED=Be^b_Tcbc^bY`d^bc[aaaaEEEEEEEEDECEEEELEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8691:10101#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":85,"pairing_fast_score":0.817,"pairing_mismatches":{"(A:12)->(G:23)":73,"(C:02)->(T:34)":106,"(G:02)->(C:34)":101,"(T:34)->(G:08)":36},"score":3093,"score_norm":0.953,"seq_a_single":23,"seq_ab_match":81,"seq_b_single":23}
ccacgagagaggatatccgttgccgagagtcatattttttttctgttggcctcggcagccgacggagccctcgaaaggctcgccggtgccttagctttaccgtgttccttggcgtcatccacgtctcgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCEacccbecLZJMFQYb[[d_aUabeadab`cac[STcdbbd\cdacdcd:ceEEEEEBEEFEEEEEEEEDEEEEEEELEEEELEECCCCCCDCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8892:10485#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gaatggt:gaatggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatggt","obimultiplex_forward_tag":"gaatggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatggt","obimultiplex_reverse_tag":"gaatggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:13)->(G:34)":47},"score":1655,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCC;CCC?BCCCCCBDBDZc`\cYNXaeddcb\]`SCXQWPFTa][E`CEDCC:?CDEEBEEEEEEEEEEEEEEELCEEBECCCCC@CCCCCCCACCCC
@HELIUM_000100422_612GNAAXX:7:1:8655:12483#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:02)->(G:34)":87},"score":4286,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccactgcgtagccattgagtctctgcacctatcctttttgattttcgctttctgaatctttgcttgttttcggaaaacgtgatttggctcaggattgccctgagagcgtgg
+
CCCEEEEEEEEEcTae_acee___`_]^Y^\c[ccccU__`eebacabeeafdc_][aa^c\T^\d`dd`UYN\\bcEEEEEEEEELEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:85:8513:10615#0/1_sub[28..127] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (attaact:aattaac)","obimultiplex_forward_error":1,"obimultiplex_forward_match":"taagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"attaact","obimultiplex_forward_tag":"attaact","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":3752,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCEDEcUZXV[eddeY\bbcYbba\[]a^\\XVY[addccUS^WVceeedeaeYf_`baS_c]CCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8687:1776#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":101,"pairing_fast_score":0.959,"pairing_mismatches":{"(G:09)->(A:33)":79},"score":4912,"score_norm":0.99,"seq_a_single":7,"seq_ab_match":100,"seq_b_single":7}
ccatcagctgcgggaaatcctgagccaaatccgtgttttgagaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggcagctgatgg
+
CCCCCCCXee[dce`_aZT_`ceeTeecedbdeeebaeadd__bdebddbdeccbceeeaeea^ZY]\X[\ca`]c_cEcEEEEEEEEFEEEEEEEEEEEEEEEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8639:18202#0/1 {"ali_dir":"right","ali_length":76,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":73,"pairing_fast_overlap":76,"pairing_fast_score":1,"score":5019,"score_norm":1,"seq_a_single":32,"seq_ab_match":76,"seq_b_single":32}
cactctttccctacacgacgctcttccgatctcgtcgtacgggcaatcctgagccaaatcttgtttctacgacgagataggtgcagagactcaatgggtacgacgtggagatcggaggagcggttcagcagaaatgcgga
+
<<?>B6AB100*+A?A3?BAC@CAAAA;@=BAcTXY\Y]c\\aebdeeee_e_eebeeeefeedeeeceeddbdeecfeeeee]beeee_eU[\Z\Z^YaY\RRRRQ_BAA#############################
@HELIUM_000100422_612GNAAXX:7:1:9113:2556#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atgcaga:gtgacgt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgcaga","obimultiplex_forward_tag":"atgcaga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgacgt","obimultiplex_reverse_tag":"gtgacgt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(g:15)->(t:34)":81},"score":3114,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCACCCC@CCCCCCCCCCELEEce_]Ucc_bRPGQQR]_cX_____TWXYZ`^aacY\`OYVRRRLQZ^Y\\V]ZLYa_DCCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8920:7878#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtaggt:tgtaggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtaggt","obimultiplex_forward_tag":"tgtaggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaggt","obimultiplex_reverse_tag":"tgtaggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{},"score":1650,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ctagccgtaaaccttgatagaaatatacaattgatatccgccagggaactacaagcgccagcttaaaacccaaaggacttggcggtgcctcagacccac
+
CBBCCCC?CCCBCCCC?CVedeecfc_beeWb_]_aY\cbIebECEEEE?@EDBEEDEEEEBEEEEEEEEEELEELAEELECDCCCDCCCCCC9CCCAC
@HELIUM_000100422_612GNAAXX:7:1:8700:19125#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":102,"pairing_fast_score":0.717,"pairing_mismatches":{"(A:34)->(G:02)":27,"(A:34)->(T:02)":23,"(C:06)->(G:20)":103,"(C:27)->(T:02)":47,"(C:31)->(G:02)":19,"(G:34)->(T:02)":11,"(T:34)->(C:02)":37},"score":1934,"score_norm":0.931,"seq_a_single":6,"seq_ab_match":95,"seq_b_single":6}
ccacgctcatggggcaatcctgagccaaatccgtgttttgagaaaaccaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcatgagcgtgg
+
CCCC@CEEDELCEDEEEDIEDELEEELEEEEEEAEDLEED?B:C?7EA>A98>,B=@C@?AEAAA=Ec`Ta`a_P\dab[]RN]LD;QPHQ\PWW]Z`U`Z]9bC@8D???BBB
@HELIUM_000100422_612GNAAXX:7:1:8709:8686#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gaagtag:acaacaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaacaa","obimultiplex_reverse_tag":"acaacaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1861,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCBCBCCACebeebc``\edZeed\be_^^_Wa^caEA@<>;DEBEEDAECAEEEEEEEEEEEEEELEEELCCCCCCBCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9153:8634#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gatgacg:gatgacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gatgacg","obimultiplex_forward_tag":"gatgacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gatgacg","obimultiplex_reverse_tag":"gatgacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1569,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCd_edddbddcbddd]W\c^V`[aEEEEEDDB:DEEEECEEEEEEEEEEEEEEEEEEELEEELCCCCCCCCCCCCC@CCCCB
@HELIUM_000100422_612GNAAXX:7:1:8678:18956#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(T:02)->(G:30)":101},"score":5725,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
cccacgtgacagggcaatcctgagccaaatcctgttttccgaaaaccaagaagagttcagaaagggagaataaaaaaaggataggtgcagagactcaatggtgtcacgtggg
+
CCCCUW^ceccedTaN_eXbbeecXedeebedbe`ecdUeedeeedcedecceecXca_`eeeccceb_a^^cc_\_[dWZYXYOYXVVSaEEEEEEEEEHEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8693:6604#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":5602,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
ccagagagcacgggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggtgctctctgg
+
CCBCCACCb`bQORILLZYaMWb`Qcbaeb`ecec`a_d[_][bbdbb\][b`e^V]Od_a`W`_`_`b]dcdddbeV__UP\LU`ZRSR``_Y`beV`bEEEEEEDECCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8637:3447#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":106,"pairing_fast_score":0.99,"pairing_mismatches":{"(T:02)->(G:34)":108},"score":5440,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
ctccagatcgacggggcaatcctgagccaaatccttctttttggcaaacaaataaggattccgaaagaaataagggataggtgcagagactcaatgggcgtactctggag
+
##EEEEEEEEEEEe]]bbddcbbccaY_e_ecTdddeee]dddeddedeeeddeeeeadceedddeeeedddeeaeea^^[`\YY]^e`UbEEEEEEEEEEEEEEFFL##
@HELIUM_000100422_612GNAAXX:7:1:8696:6438#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":3481,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ctacccgaccctcctcccatccccagctgacgcgggcaatcctgagccaaatcctttttccgaaaaaaaaaaagggataggtgcagagactcaatggcgtacacgtggagatcggaagagcggttcagag
+
######################EEEEEEEEEEEEEEEEEEBEEEEEEcecbc^ebeeaaeeWdddddeddd_]deadWY^]Lec^eebbRa_Y`[\WYaEEEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8648:15811#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":103,"pairing_fast_score":0.91,"pairing_mismatches":{"(A:33)->(T:16)":42,"(G:34)->(A:02)":6,"(T:02)->(C:34)":101},"score":3977,"score_norm":0.971,"seq_a_single":5,"seq_ab_match":100,"seq_b_single":5}
ccagagctctgccattgagtctctgcacctatcctttatttattctcgctttatgaaacccctgtttgttttcataaaacgggatttggctcaggattgccccagagctctgg
+
CCCCCLEEEEEEEEEccPadadbZPYNNc_ac_QQYYLMTVCPc_c__dcedecdbebeebec`]`ee`eddddNceeecEEEEEEDEEEEDEEEEEEEELEEDEAEBBBCCC
@HELIUM_000100422_612GNAAXX:7:1:8677:6489#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":105},"score":3606,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccagacgtacggggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgtacgtctgg
+
CCCCCCCCRZ[bcc]Qc`Y__eab]eebe]abddc`efdbbd^ddeddddbdbeeeeaeeEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9075:19338#0/1_sub[28..127] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtaaca:tgtaaca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtaaca","obimultiplex_forward_tag":"tgtaaca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaaca","obimultiplex_reverse_tag":"tgtaaca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":1306,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCBCCATcaebeeedcee^cdXMUaFEEEEEEEEEECCECEEDEECEEEFEEEEEEEEEEEFEEEEEELCCCCCCCCCCCCC=CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8650:20247#0/1 {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(T:02)->(C:34)":97},"score":2634,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
atgcaggaaggaagtttgagcttgatgcaaatggctaaaatatcgtctgctttatatgattatcaattaaataaaaagttattttatgtatcaatccttacatctccgacaactggtggagtgacagctagtattggtatgttgggggatatc
+
CCCCCCCCCCCCCBCCCCCCCCCCCCCCBCCCCCCBCCCCCCC@CTWY`Z_ac\T[UTSV]\L[``ZVXWbaXbb[[WM\b_bUWY^X\`EECDDDLDCFED>EDDEDBCB@CDBCBCCCBCDDCCCCCCCC<CCDCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8646:12450#0/1 {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":63,"pairing_fast_score":0.833,"pairing_mismatches":{"(G:34)->(T:02)":47,"(T:02)->(A:31)":87,"(T:02)->(A:34)":94},"score":1690,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
gatatcccccaacataccaaaactagctgtcactccaccagttgtcggagatgtaaggattgatacataaaataactttttatttaattgataatcatataaagcagacgatattttagccatttgcatcaagctcaaacttccttcctgcat
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC=CCBC@>CC?EL_^d\`aZR]YUbb^X_ZaY]]Q^aac_::.;A?EBBBAEIBCEEE>LAEEEEEEECEEEECCCCCCCCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9275:7244#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttatgtg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatgtg","obimultiplex_forward_tag":"ttatgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:02)->(g:34)":77},"score":2945,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCCCCCCeCdd_]dd\bb[bXYLX[OYWYKZWZZYPW[XWa[YYZSEMKER[UYYSWaaU`a`ELDEDECCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8692:4088#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":104,"pairing_fast_overlap":107,"pairing_fast_score":1,"score":5376,"score_norm":1,"seq_a_single":1,"seq_ab_match":107,"seq_b_single":1}
ttgcgtcgggcaatcctgagccaaatccgtgttttgagaaaacaaggggttctcgaactagaatccaaaggaaaaggataggtgcagagactcaatgggacgcagatgg
+
#c[ca`c^ZeceebRe]ecaeeeecceedeeeeeedebbeeeeeddceececeadeeaddeeebabcccc\cccadCEEEEEEEEEEEEEEEEEDEEEEEEEEEEEEE#
@HELIUM_000100422_612GNAAXX:7:1:8663:3156#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":92,"pairing_fast_score":0.955,"pairing_mismatches":{"(A:02)->(C:34)":102},"score":5032,"score_norm":0.989,"seq_a_single":16,"seq_ab_match":91,"seq_b_single":16}
ccaacattgattcggttggggcgacctaggaataatcatcctaaaaataaaagacaaattagtctatacaatgaacatttaaagatttaaaatcaagctaccttagggataacagcaatgttgg
+
CCCCCCCCCDCCCCCCcSc]]Y[^b`cdWeeceeeZeeeeedcdddce]dddd\ecdddcRRORQcdc`_ce]_ddb_db_c_c]eeabeaEEEEEEEEEELEEEEEECCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8704:15923#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":90,"pairing_fast_score":0.92,"pairing_mismatches":{"(A:02)->(C:34)":99,"(C:02)->(A:34)":106},"score":5118,"score_norm":0.978,"seq_a_single":18,"seq_ab_match":88,"seq_b_single":18}
acgacgctcttccgatctccttcgagtttagataccccactatgcttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccactcgaaggagatcgcaggagcggttc
+
##########A:?2@CCCMZYZSbebeebe`cebeeee_eee_deeecececbeeefeeeeceeedaddcddc_dd__\dZeeYc\_c]cccEEEEEELEEEEEELEE##################
@HELIUM_000100422_612GNAAXX:7:1:8681:13269#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":3179,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
cccagactgtcgggcaatcctgagccaaatcccgtttttcgcaaaaacaaagaaaagttcagaaagctaaaataaaaaggataggtgcagagactcaatgggacagtctggg
+
==ABCDDDDCDDD@A@AA:CCDda\cc``a[X[`W\\YX\>UY[JcR_WXMUXWUZZXd^Xa``]^a\\X__ddEEEEEEEEED.DDECEEEEEEDDEEEEECDEEEECCAB
@HELIUM_000100422_612GNAAXX:7:1:8886:1574#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4333,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
agagg
+
eeded
@HELIUM_000100422_612GNAAXX:7:1:8704:17783#0/1 {"ali_dir":"left","ali_length":82,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":82,"pairing_fast_score":0.734,"pairing_mismatches":{"(A:28)->(G:02)":40,"(G:26)->(T:02)":54,"(G:31)->(A:02)":38,"(G:31)->(T:02)":49,"(G:34)->(T:02)":65,"(T:32)->(G:02)":50,"(T:33)->(G:02)":42},"score":243,"score_norm":0.915,"seq_a_single":26,"seq_ab_match":75,"seq_b_single":26}
tcgcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgcggtgtgcattccgagcgtgtcatcttttgaatcacaaacgactctcggcaacgaatatcgcgacacgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCDDEEEE@EBEEI=FCK?DCCACIJ<C?D?EEDE?EBCEL\YV]REACE>DCC8E?EEDC17CAEEEBCD:CDBA<A94EE;C=CBCC@CCCCCCCCCCCCCBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8697:11711#0/1 {"ali_dir":"right","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":87,"pairing_fast_score":1,"score":5897,"score_norm":1,"seq_a_single":21,"seq_ab_match":87,"seq_b_single":21}
tacacgacgctcttccgatctccagatcgacggggcaatcctgagccaagtccggtgaagggcgaaaagtaaccggataggtgcagagactcaatggcgtcgatctggagatcggaagagcggttcagc
+
##########CC?CAC+CC;Ceceeebefcbeeecebdebecddeeeececeeeedefaeeeeefeece]ebc\a`\\YZOU^[^^c`_]cZa`N_cee_]P[Y\YcaAC@##################
@HELIUM_000100422_612GNAAXX:7:1:8687:5927#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4339,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
cagcgatcaggggcaatcctgagccaaatccttctttctgaaaacaaataaaagttcagaaagttaaaatcaaaaagggataggtgcagagactcaatggctgatcgctgg
+
CACEEEc`cee____\]WYWVe`aeac_cc`cdeecdb`dbdeedccddded_cad]^caccc_]UaaaEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8632:7589#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":107,"pairing_fast_score":0.962,"pairing_mismatches":{"(A:02)->(G:34)":85},"score":5330,"score_norm":0.991,"seq_a_single":1,"seq_ab_match":106,"seq_b_single":1}
ccacgcgtcaggggcaatcctgagccaaatccttctttctgaaaacaaataaaagttcagaaagttaaaatcaaaaagggataggtgcagagactcaatggctgacgcg
+
Ce`eZZVYYYP[KYa]_^]c]]ecaeeceeSecbeebceabbdeeddcddce`\d_ccbdeeedadadddc__ccd_d_dEEEELEEEEEEEEEEEEEEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8882:15905#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:32)->(G:16)":32,"(C:02)->(A:34)":59},"score":1659,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCBBCCCCCCCCCCCCCCC``a`[VJYHSNVBGMWUYIP]?PYUSPQILRCQZ^^?A;LCEE=EEEEEEED>EHEELDE>DCCACCCCBCCCC@B@BCCC
@HELIUM_000100422_612GNAAXX:7:1:8621:2319#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":98,"pairing_fast_score":0.958,"pairing_mismatches":{"(A:02)->(G:34)":100},"score":3854,"score_norm":0.99,"seq_a_single":10,"seq_ab_match":97,"seq_b_single":10}
ccatgcgagacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccctttctttgttttctcaaaacacggatttggctcaggattgcccgtctcgcatgg
+
CCCCCCCCCCEEEDEEEEEEEEEEe]dacb\XXeaeee^edefcc`ZdZc]ddef[eedddeeadeeddd]dcdd\`_aEEEEEEEEEEEEEEEEEEEELEEEEEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8623:13612#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":57,"pairing_fast_overlap":89,"pairing_fast_score":0.663,"pairing_mismatches":{"(A:02)->(C:23)":106,"(A:02)->(G:19)":95,"(A:34)->(G:02)":49,"(C:02)->(A:31)":98,"(C:02)->(T:27)":80,"(C:26)->(G:02)":32,"(T:02)->(C:24)":78,"(T:02)->(G:15)":107,"(T:28)->(A:02)":26,"(T:31)->(A:02)":20},"score":174,"score_norm":0.888,"seq_a_single":19,"seq_ab_match":79,"seq_b_single":19}
ccaacacactacaaccaaatccaatttcacgccaatattaaaaccaacaatccaaataaacaaatatcaatgtaatccatcattccacttagaagcaagctgcaccgtgacctgaaatgtgtgttgg
+
CCCBCBCCCCCCC:CC@CCIEEEBEFC??A8D78;EEEEEBEAEEEEELEEA:EE??<^ZZ_>>C>3BB>;34::)5B)E>:5;BCA>97>=BB=B6IBBBB?6>A9==(<<@9@@@@@@@@@@@@@
@HELIUM_000100422_612GNAAXX:7:1:8651:6951#0/1 {"ali_dir":"right","ali_length":67,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":67,"pairing_fast_score":1,"score":4889,"score_norm":1,"seq_a_single":41,"seq_ab_match":67,"seq_b_single":41}
cgagatctacgctctttccctacacgacgctcttccgatctcccagagcgatccgaaggcgtcaaggaacactctggggactctcggcaacggatatcatcgctctggagatcggaagagcggttcagcaggaatgccgagacagatat
+
#############B,2@:AC9?CAA@AB9CCC=CA?@?CC@eeeeeebbdaeeeeeeeeeeefaeeeeeegededeeecdfdeeefeefeeeeeebea]e]eede]ecCC@.7809/AA?>?;?#########################
@HELIUM_000100422_612GNAAXX:7:1:8620:9668#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":100,"pairing_fast_score":0.918,"pairing_mismatches":{"(A:02)->(G:34)":98,"(T:16)->(C:30)":76},"score":4346,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
ccacgactagcccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccctttttttttcccaaaacacggatttggctcaggattgccccagagtcgtgg
+
CCCCCCCCEEEEEDEEEEFeecc_bc`Ta\_e\ebeccNcaebdeeccabZdeee]eedeeeeedcddeeedd[d@X`cc`aaEEEEEEEEEEEEEELEEEEEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8886:11010#0/1 {"ali_dir":"right","ali_length":52,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":45,"pairing_fast_overlap":52,"pairing_fast_score":0.918,"pairing_mismatches":{"(A:34)->(G:06)":72},"score":3689,"score_norm":0.981,"seq_a_single":56,"seq_ab_match":51,"seq_b_single":56}
tgatacggcgaccaccgagatctacactctgtccctgcacgacgctcttccgatctccacgtaccttagataccccactatgctagaggagcctgttctatgcgcaggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgcggtctcct
+
#################################?;5'<=@=5ACCCC@CC2AC:<3^\ceeeaeeeeedbdGcebebeeeedeedeeedeebeedeeeeeeeceddeeCCCC@ACCCCCB@CA?=A89979AAA;=7/779?######################
@HELIUM_000100422_612GNAAXX:7:1:8959:2411#0/1_sub[28..127] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:ggtgaga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggtgaga","obimultiplex_reverse_tag":"ggtgaga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":61,"pairing_fast_score":0.931,"pairing_mismatches":{"(a:02)->(g:34)":77},"score":2387,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
ttagccctaaacacaagtaattattataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCBCCCCCCCCCCCCCC8CaO_b^bcZV`aabW\JZ^]OVaITUQWRNRZOKZ\\SZ^Z`^CEEEEEEAEEEEEEELEEBCCCCCDCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8960:20749#0/1_sub[73..81] {"ali_dir":"right","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtccga:tgtccga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtccga","obimultiplex_forward_tag":"tgtccga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtccga","obimultiplex_reverse_tag":"tgtccga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{},"score":3036,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ggccgcgtg
+
dYcK`ca]d
@HELIUM_000100422_612GNAAXX:7:1:8761:3724#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tatctca:tatctca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatctca","obimultiplex_forward_tag":"tatctca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatctca","obimultiplex_reverse_tag":"tatctca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4362,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
ggcct
+
eeeef
@HELIUM_000100422_612GNAAXX:7:1:8680:17726#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(A:02)->(G:34)":93},"score":5087,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccacatctgcgccattgagtctctgcacctatccttttttattttcgctttctgaaccttcgtttgttttcggaaaacgtgatttggctcaggattgccctacgctgctgg
+
CCC_dd[U``bRORRQIYMZZ\[]I\[`^]acTddRd\`bed`dddd^eddeddebdcc`c^^Z[ecc\`WU[]aIa__]QLRRScK_cEEELEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:74:16256:18027#0/1_sub[29..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aatatct:gaatatc)","obimultiplex_forward_error":2,"obimultiplex_forward_match":"taggataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatatct","obimultiplex_forward_tag":"aatatct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gaatatc","obimultiplex_reverse_tag":"gaatatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(G:25)":48,"(A:34)->(G:16)":33,"(G:02)->(C:34)":63,"(T:02)->(G:17)":70},"score":525,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtgctactagcaacagcctgaaacgcaaaggacttggcggtgctttacatccct
+
BCCCCCCCCBCCCCDCCCEEEFccYcZLIUZJDXJ[?6EAE?AAAA.C9<5CD7EBEE;AE<L8CECCC;?>ELDHFEAECCCCCCCACCCCAB?CCCC
@HELIUM_000100422_612GNAAXX:7:1:8683:12609#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":105,"pairing_fast_score":0.99,"pairing_mismatches":{"(C:02)->(G:34)":108},"score":4819,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
tctccacactctgcgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaggataggtgcagagactcaatggtgcgtctgtggaga
+
###Ec[c`]c_]cQQOROa]^bebc`cec`Qaa[_`[ZcdcdZadceedccb_]]QYb_`a]accacaccbcccd\_cEEEEEEEEEEEEEEEEFEEEEEEEEEEEFL###
@HELIUM_000100422_612GNAAXX:7:1:8703:4280#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":105,"pairing_fast_score":0.775,"pairing_mismatches":{"(A:02)->(G:34)":100,"(A:10)->(G:34)":79,"(A:34)->(G:02)":47,"(C:02)->(T:34)":106,"(C:34)->(G:02)":39,"(G:34)->(A:02)":13},"score":1907,"score_norm":0.943,"seq_a_single":3,"seq_ab_match":99,"seq_b_single":3}
cccagacgagtgggcaatcctgagccaaatcctgttttctgaaaacaaccaaggattcagaaagttataataaaaaaggataggtgcagagactcaatggactcgtctggg
+
CCCFEEFEEEEELEEEEEEEEEEEEEEEEEEEDEEEEELDDEEEEEL7D?CCCA?C@CbZba\^G\cZ^Z[bee\Y]aFaUUZUY^\T^\cEEEEEEEELEAEEEL@ECCC
@HELIUM_000100422_612GNAAXX:7:1:8616:6909#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":89,"pairing_fast_score":0.86,"pairing_mismatches":{"(A:02)->(T:34)":107,"(C:34)->(A:02)":32,"(T:02)->(C:34)":105,"(T:34)->(C:02)":43},"score":2412,"score_norm":0.955,"seq_a_single":19,"seq_ab_match":85,"seq_b_single":19}
ccgaatatcattcggttggggcgaccaaggaccacatcatcctttattacaaagaccattaagtcatacaaagacccttaaataagatcaccaaataaagctaccttagggataacagtctcaccgg
+
CCCCCCCCCCCCCDCCCCCEEEEEEEEEEEELEEEEEDDEEELEEEE]^dXd`dbZcaJ_\XYZ^\`]]]eee`aaZ]U^[\]XSaEEEEEDDEEEEEEEEEEELELECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8675:18231#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":98,"pairing_fast_score":0.958,"pairing_mismatches":{"(A:02)->(G:32)":100},"score":5258,"score_norm":0.99,"seq_a_single":10,"seq_ab_match":97,"seq_b_single":10}
ccagcacacagccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccctttctttgttttctcaaaacacggatttggctcaggattgcccctgtgtgctgg
+
CCCCCCCCCCEEEdcaUcdZede\]`^Xb_cdacccccccUaeaddcbceb_dd_`eebeeed[deedddbddedddeba`ccccaaKaEEEEEEEEEEJEEEEDEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8636:4826#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":3275,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gctgccccctatatctacactccttccctacaccacgctcttccgatctcctggcgcaattcaggtcaaggtgcagcggtgaaattggatttggttgtagccaccaggagatcggaagagcggttcagcaggaatgcggagacggatatcgtatgcg
+
#####################################BC=C@C9;,<;8\\[]_bTaUbbddb[]aa_aVSXZWa_]cdb`^dYaddd^dad`c\``_bEEEAEEEEE#################################################
@HELIUM_000100422_612GNAAXX:7:1:8669:6372#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":93,"pairing_fast_score":1,"score":6168,"score_norm":1,"seq_a_single":15,"seq_ab_match":93,"seq_b_single":15}
gcgctcttccggtctccagagctctgccattgagtctctgcacctatcccccttttcactttgttttcggaaaataggatttggctcaggattgccccagagctctggagatcggaagagcgg
+
#A7@??+?.85(7B@dde]eaeeb`ee`ececaQQQQRace`defeeeeceeeb_ee[dddeeee_ddabbeb_deebcaecebc]^[^[c^bcceece]cc^TdEEE###############
@HELIUM_000100422_612GNAAXX:7:1:8637:13235#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":100,"pairing_fast_score":0.68,"pairing_mismatches":{"(A:34)->(C:02)":27,"(C:02)->(A:23)":81,"(C:29)->(G:02)":10,"(G:27)->(A:02)":9,"(G:34)->(T:02)":44,"(T:02)->(G:32)":98,"(T:31)->(G:02)":45,"(T:34)->(G:02)":50},"score":891,"score_norm":0.9,"seq_a_single":8,"seq_ab_match":90,"seq_b_single":8}
ccagtcgagctccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccctttttttttctcaaaacacggatttggctcaggattgcccagctcgactgg
+
CCCCCB<@EG21112EEEELEEEEEELEEEEEEEABEEBBBB<LIEEEELEEE4BD@@*>=E_]][ZW\_^]UMSYVL\@A?CBCJC<CCCCBCCCCJCCB@@@@3CC?AAAAAAA
@HELIUM_000100422_612GNAAXX:7:1:8641:6663#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5608,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccacgtcagtcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccccgtacacgtgg
+
CCCCCCEEEEccbd\`a`cUc[__U`_]_]caZbedcedc__ccZeaZcd_ddedcbeebeeedddeebeeedeebcebeeeda[cabcScEEEEEECEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8699:5491#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(A:02)->(G:34)":84,"(T:02)->(G:34)":100},"score":5372,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccagtagctggggcaatcctgagccaaatcgcgttctccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaaagataggtgcagagactcaatggcagctactggg
+
CCCWYX_ccXcI__c_]_b\_eededeeefeea_eeeeecbdeedddddfeddeeeb`ecdeedcdbdbddcccddd_ddEEELEEEEEEEEEEEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8624:5516#0/1 {"ali_dir":"right","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":73,"pairing_fast_score":1,"score":5283,"score_norm":1,"seq_a_single":35,"seq_ab_match":73,"seq_b_single":35}
ctacactctttccctacacgacgctcttccgatctcgacacgcgtgccattgagtctctgcacctatccctgtgtgcattggctcaggattgccccacgcgtgtgtggagatcggaagagcggttcagcagaaatgcggagac
+
##################A+3BACBC=BABA9AACcX_e`eeceedcefeeaeeeefeeeeeeeebeeeeeeeeefed_eeeee]\aa_aecceceeeeeeeeeedeaCC<33::C=C@C??9?###################
@HELIUM_000100422_612GNAAXX:7:1:9143:3777#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ggttatt:ggttatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggttatt","obimultiplex_forward_tag":"ggttatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggttatt","obimultiplex_reverse_tag":"ggttatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4183,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCCCCCCCCC_ccdcdWaeeace`dd_fcaef_ebdce\Z[MQaaaeacbb_T`eeaeededec\aY]aYUYCDCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8704:7838#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":103,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(G:33)":86,"(C:02)->(A:33)":99,"(T:02)->(G:33)":102},"score":965,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
ccagacgagaggggcaatcctgagccaaatcccgttttatgaaaacaaacaaggatttcacaaagcgagaataaataaaggataggtgcagagactcaatggctctcgtctgg
+
CCCCCEECEEEEEEEEEEEEEEEEEEEEEEEEEE:EEEDEECEEEEEB@BDEE<BEABac^]cb]YUOUV\NR^D7DDDDKDDDDKDDDDDDDDDDDDKDDKD?@DDDBBBBB
@HELIUM_000100422_612GNAAXX:7:1:8640:20230#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":60,"pairing_fast_overlap":90,"pairing_fast_score":0.69,"pairing_mismatches":{"(A:34)->(G:02)":56,"(A:35)->(T:02)":45,"(G:23)->(C:02)":70,"(G:34)->(A:02)":29,"(G:34)->(T:02)":19,"(T:02)->(C:34)":107,"(T:34)->(G:02)":33,"(T:35)->(A:02)":44},"score":629,"score_norm":0.889,"seq_a_single":18,"seq_ab_match":80,"seq_b_single":18}
ccactgctgcagatatccgttgccgagagtcgtgtggattaaatacgattgcaacacaagagatgaccagcaagctagccatgccctcgggttagacacagtgttccttgacgccttcggtgcagc
+
DCCDCCCCCCCCCCCBCCLLEEEEDELDLEE?LECECCEEEECMMAE?EEE=CBBLCDEACA?+.:9:;A;;UZXVMKSVX][\bEECEEE4EEEBEBEEBEEEEELECCACACACACCCCCCC?C
@HELIUM_000100422_612GNAAXX:7:1:8609:13895#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":89,"pairing_fast_score":0.884,"pairing_mismatches":{"(A:02)->(C:34)":97,"(C:02)->(T:34)":107,"(G:34)->(T:02)":29},"score":1904,"score_norm":0.966,"seq_a_single":19,"seq_ab_match":86,"seq_b_single":19}
ccttcgccaattcggttggggcgaccgtggaattcatcatccaccacaaataagattatctaatcactcaaaagatcctaacaatagatcaaagaccaagctaccttagggataacagtggcgaagg
+
CCCCCCCCCCCCCDCCCCCEEEEEEEDELEEEEEEFEFDEEEDEAEEcc`\bb_^\cebd_a^]_^Z^]]eeeeaECEEEEEEFEEEEEEEEEEEELEEEEEEEEELFCCCCCCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8687:8141#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":4661,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccgaagtagtcaagaacataactaatactactcaggcacaaaatttaacgctccaaaataatagggtatctaatcctagtctacttcggagatcgcaagagcgcttca
+
;;79=3A=CC6C?A2=A?CcUbea_ac\cebeeeeeefecdcecd\bdafdecbededdeeeeeedeefbbbdbeeeedc[c]cEEEEEEEEEEEEEEEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8689:5728#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(C:25)->(G:18)":87,"(T:02)->(G:34)":100},"score":5674,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccacgacgcgagggcaatcctgagccaaatcacgttttccgaaaacaaacaaagattcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggtcgcgtcgtgg
+
CACEEEEaaUbdYeed^PU^WacWZeeaece`eeb^acbZ`bcff_abbaedZ^`baZccebe]^bcdebeZb_ebbb^_c_]SaZ;YUaae\bEEEEELEEEDEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8665:18975#0/1 {"ali_dir":"right","ali_length":66,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":66,"pairing_fast_score":1,"score":1588,"score_norm":1,"seq_a_single":42,"seq_ab_match":66,"seq_b_single":42}
acgatatctacactccttccccacacgacgctcttccgatctagtctctgcacctatcctttgttttcttaacataaagatttggctcaggatgcccgcatgtgctggagatcggaagagcgcttcagcaggaatgccgaaccgatatcg
+
##########################################EECECEDEEEEBCEC:DDDDEECBB@E@^ccQQQQFVY\\LNL=I\_RWP?OPPOccc^aCCCCCC##########################################
@HELIUM_000100422_612GNAAXX:7:1:8691:18758#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":107,"pairing_fast_score":0.962,"pairing_mismatches":{"(G:34)->(T:02)":18},"score":4811,"score_norm":0.991,"seq_a_single":1,"seq_ab_match":106,"seq_b_single":1}
tccacgagacgtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccacgta
+
#EEEEEEEEEEBEEDEELEEEa]cZcc\d`ad``ceTedacaecbcee[eZe\bce`ebcYeec_abdedebbbc_aeeXdbeb]Z]M]YRaEEEEEEEEEEE?EEEE#
@HELIUM_000100422_612GNAAXX:7:1:8910:7581#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acaacaa:acaacaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acaacaa","obimultiplex_forward_tag":"acaacaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaacaa","obimultiplex_reverse_tag":"acaacaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":2680,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCBCCCCCCCCCE@DDEEEFDEEEEDDDEEECadZ_^aaaYLWWUYca_d`db]^a]Z`ccbeQb]S]]U\c`SCCCCCCDCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8958:18879#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atgatca:atgatca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgatca","obimultiplex_forward_tag":"atgatca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atgatca","obimultiplex_reverse_tag":"atgatca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(g:34)":77,"(g:02)->(t:34)":81},"score":2464,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCELEEEEEDEa`Yc^]KYaYTR\IDNQRNIHOEL[a[G[[Z\VNL[L^Q^dadWccWcLDEELCCCCDCCBCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:9141:18839#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtattc:tgtattc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtattc","obimultiplex_forward_tag":"tgtattc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtattc","obimultiplex_reverse_tag":"tgtattc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:06)->(a:34)":73},"score":1787,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCC=CCCCCCCCCCCCCCLEEEEEEEEEEEBE?DE@DEEBE?E=C?<2?A=A][\bcbd^RZa_c_P_^]]_Gd\Zd^dc]DCCCCACCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8663:14090#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:02)->(G:34)":82,"(G:34)->(T:06)":33},"score":3492,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagtcagcgtgggcaatcctgagccaaatccgtgttttgagaaaacaggggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggacgctgactgg
+
CCCCCCEEEFEEEEEEEEDEEa_T][aa[PRLGPYKY[\_e]ebdae``ceebe]cab\cec^\c`c[cc`_Y_cccEEEELECEEEEEEEEEEEEBEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8765:3292#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgcca:gcgatga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgcca","obimultiplex_forward_tag":"ttcgcca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgatga","obimultiplex_reverse_tag":"gcgatga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(A:34)->(T:02)":19},"score":1910,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCBBCCCCCCCCCCCDCCLcac]eacaebZY^_YXYVa`_^_^cbcW[=<5BBEBEEEECCDCDDADEEDEEEEEKAEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8665:1721#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":104,"pairing_fast_score":0.96,"pairing_mismatches":{"(G:34)->(C:02)":8},"score":4903,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
atcgtgcgtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccacgcacgatgg
+
CCCCEEELEEEFadZdbSWW]TMc[KcbdeWf^`c`ZbbefeRcddb^`_bde[beedcbee```db`d`[ec^]b\`c[SPW]VbbJaEEEEEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8920:10866#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgcgaa:gcgcgaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgcgaa","obimultiplex_forward_tag":"gcgcgaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcgaa","obimultiplex_reverse_tag":"gcgcgaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":62,"pairing_fast_score":0.983,"pairing_mismatches":{"(G:34)->(A:02)":20},"score":1524,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagttctaaacccaaatagttcaaccaacaaaactattcaccagagtactacaagcaacagcttaagactcaaaagacttggcagtgctttatatccct
+
CCDCCCCCCCCCCCCCCCCL?EABe`bde]eebb^\b^^^^^___aEE@=EBBAEBEEEEE>EEEFEEEEEEEEEEECEEECCCCCCCCCCCCCA?CCCB
@HELIUM_000100422_612GNAAXX:7:1:8622:12031#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":105,"pairing_fast_score":0.912,"pairing_mismatches":{"(C:02)->(T:34)":96,"(G:02)->(C:34)":108,"(T:34)->(G:02)":15},"score":3975,"score_norm":0.971,"seq_a_single":3,"seq_ab_match":102,"seq_b_single":3}
cccgacgcagtccattgagtctctgcacctatccttttttattataactttctgaatccttggttgttttcagaaaacaggatttggctcaggattgcccactgcgtcggg
+
CCCEEEEEEEED@ALEDc[c_aY\UaZZZYZ`c^Z_b]bee^a`db\edeceed`Zbc^`ccSbWc_ccSW\_aa`cdEEEEEDEEEEEEEEEEELEEEEEEEEEEELCCC
@HELIUM_000100422_612GNAAXX:7:1:8637:4688#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":5602,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
ccactagctcgccattgagtctctgcacctatccttttttgattttcagtttctgaacttttatttgtttgttttcggaaggaaggatttggctcaggattgccccgagctagtgg
+
CCCCCCCCX[`__]__bb\`dc_\a_TK]^^eceee__Za[cceededdeeededUadbdeed`ddccdddeedd`bbb_ZZ][ZJRRRQcZ_aEEEEEEEEEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:12564#0/1 {"ali_dir":"right","ali_length":88,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":88,"pairing_fast_score":1,"score":4953,"score_norm":1,"seq_a_single":20,"seq_ab_match":88,"seq_b_single":20}
acacgacgctcttccgatctccaatgtccggtcgccccaaccgaatttaaaaaatctattaggtgattttatgaataaaagctctatagggtcttcttgggacattggagacaggaggagcggttcag
+
2AAA;9A;=A=6A>?*?:CCdebeeeeee`eeca^\XbXdeedeeeeeeeeeeedefedeeebcfceeeeee\c`ddVd[[Y`__eeceaEEEEEEEEEEEEEEEEEE####################
@HELIUM_000100422_612GNAAXX:7:1:9185:12813#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":2507,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
DCCCCCCCCCCCCCCCCBCEEELDEFEFcdddRPIMRUWWYYTY\YXWMQQQUJUNWYaaUT][X]`^_d_`aEBEDEEEDCBCCCCCCCCCCCCCACCC
@HELIUM_000100422_612GNAAXX:7:1:8689:3231#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":73,"pairing_fast_score":0.786,"pairing_mismatches":{"(G:34)->(A:02)":47,"(T:02)->(C:34)":98,"(T:02)->(C:35)":95,"(T:33)->(G:15)":69},"score":1923,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacgcacgtagcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatccgcagatgtgg
+
CCCCCCCCCCCCCDCCCCCCCCCCCCCCACCCCCCDDEEEDEEEEELADE??=ACC@_LU[XRV]_c]Cecec_\eee_MLRRRQY[Y[Y^]D>MEELAEEEEEEEDECCCCCDCCCCCCCCCCCCCDCCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:6:5300:10000#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(A:02)->(G:34)":103,"(A:31)->(G:02)":53,"(C:02)->(A:33)":100,"(C:02)->(T:34)":107},"score":965,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ccgaatatcttagataccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggaggcctgttctagatattcgg
+
CCCCCCCCCCCCCCCCCCBCCCCCCCCCCCCCCCCCCCCBCCCCCBEEDDCEIEEaWaSS__`FbVY^Z[Z_????A:?=BAECCEECACEE?EEDEEEKEELAEELECCCCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8634:7189#0/1 {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":63,"pairing_fast_score":0.933,"pairing_mismatches":{"(T:02)->(A:34)":94},"score":1282,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
gatatcccccaacataccaaaactagctgtcactccaccagttgtcggagatgtaaggattgatacataaaataactttttatttaattgataatcatataaagcagacgatattttagccatttgcatcaagctcaaacttccttcctgcat
+
CCCCCCCCCCCCCBCCCCBCCCCCCCCCCCCBCCCDCCDCCCCCCBEEEE>BEBB<D:AD<@]]_[\U[ZeceeKBNNUUba`EE3ECEEEEALEEEEEEEEEEEBEEDCCCCCCCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8631:5075#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":103,"pairing_fast_score":0.89,"pairing_mismatches":{"(C:02)->(A:34)":99,"(C:02)->(G:34)":102,"(T:34)->(G:28)":36},"score":2884,"score_norm":0.971,"seq_a_single":5,"seq_ab_match":100,"seq_b_single":5}
ccagcgcctagggcaatcctgagccaaatccgtgttttgagaaaacaagaaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtagtgcgctgg
+
CCCCBEEDDDEEE;EEDEEEEEEEc`aZ[`]a[aVCcaIbUPeeede]bc_`WU]eca_ebdZdZdee`eEEEEEEEEEEEEEEEEEEEEEEEEEEEELEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:9204:15656#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(c:02)->(t:34)":81},"score":2502,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CBCCCCCCDCBCCCCCCACELEEEEDEEebcdYWLZYUF^^M[WW\\VKP=O>CVHZXLMCAIb`^`aaf`V_]_cE>EDLCDCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8635:20422#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":85,"pairing_fast_score":0.854,"pairing_mismatches":{"(A:02)->(C:34)":96,"(C:09)->(T:34)":79,"(T:34)->(G:06)":42},"score":3949,"score_norm":0.965,"seq_a_single":23,"seq_ab_match":82,"seq_b_single":23}
ccagatcgcgaccgaaggcgtcaaggaacactatgccgaactcgtgggcgcggatggcttgccagccgccccacgcgttgcaatgctataaaatccacatgactctcggcaacggatatctcgcgatctgg
+
CCCCCCCCCCCCCCCCCCCCCCCbbea_addRcZL[c`_[`GW_ceecedda`\]\\_\]Q^[UPL`Z]dcbbdeceaF]c]P]_^WRaEEEFEELEEEEEEEFEFEECCCCDCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:10169#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":86,"pairing_fast_score":0.952,"pairing_mismatches":{"(G:34)->(T:24)":31},"score":4095,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ctacacgacgctcttccgatctccaaccaagggtcgccccaaccgaatttaatttttacttatttagtaggttaaattaagctctatagggtcttcttgcttggttggagatcggaggagcggttcagca
+
##################DCCBecc__ca_C\\TZHNZTebbedeeeeebeeceedebeebbeeedaV^Z]_]a^T^^aXaYbbaaEEEEEEEEFEEEEEEEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8721:7187#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:ggttcac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggttcac","obimultiplex_reverse_tag":"ggttcac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(T:34)":65},"score":1836,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCBCCCCDCCCCCCCCCBA`Y`W_d]]beeceecZ]caS[Y^^\bccC?CC?EFEEEEEEEFEELDEEEEEEEEFELEEELCCCCCCCCCCCCCCCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8630:3719#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":98,"pairing_fast_score":1,"score":3994,"score_norm":1,"seq_a_single":10,"seq_ab_match":98,"seq_b_single":10}
ccatgcgagacgggcaatcctgagccaaatccgtgttttgagaaaacaaagaaaggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggtctcgcatgg
+
CCCCCCCCBCYVPa]]aUP[UIUaM]S\d^Jcdbbdddddd_\Z``\ZXZ__bS\aadca]a^Pcc``adSZ`XaCDCDDDDD@=@><@@DDDDDBDDCDDDDDDDDDBBB>BBBBBB
@HELIUM_000100422_612GNAAXX:7:1:8662:20080#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":6077,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
ccactgtcgctgatatccgttgccgagagtcatattttttctgttgccttggccttggcaaccgacatagcccttgagcggctcgccggtgcctcagcttttatcgtgttcc
+
CCCC^dfbdad\[Q`\_`dccc_^]d^cY[bVZJXXfda]_`ccdcdaeb`eddabda\`b_^ab]]X_ZY_\__^_`cdU_c_c_M]IWQQPRQ`ZbaacEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:9096:4239#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aaccaag:aaccaag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccaag","obimultiplex_forward_tag":"aaccaag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccaag","obimultiplex_reverse_tag":"aaccaag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(C:33)":54},"score":1521,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCBCEEEEe_cdaeebecaG\a`___Qbb]bDC<>?@EKBEEEDEBDEEEEEBEE>BDEEELEEEKCCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:12203#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5853,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccccacacgacgctcttccgatctccttccgctggtcgccccaaccgaatctttagtcaaggtgttgatttattgataaagctctatagggtcttcttgagcggaaggagatcggaagagcgcttcagcaga
+
######?<816'6C=:A=@<;CC=ed]ecea^`dbdeedaeebaeeedeeeeedeebeedeeaeebeeeeeeeeecbecda_ebdeece_VTb_SZY\Z_aca`d]_\AB??####################
@HELIUM_000100422_612GNAAXX:7:1:9154:2101#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agccata:agccata)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agccata","obimultiplex_forward_tag":"agccata","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agccata","obimultiplex_reverse_tag":"agccata","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(a:02)->(c:26)":63,"(g:02)->(t:34)":81},"score":1262,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
C=CC@CCCCC@CBCBC?CCEAAEEEEEEB?@8?@?Eb_KXOYC_U__KYWSTMX`KU`UL^CD<D=6:;CA?E:EE=EEDLCCC?CC@D?CCDCABCDCC
@HELIUM_000100422_612GNAAXX:7:1:8618:19109#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":73,"pairing_fast_score":0.886,"pairing_mismatches":{"(C:02)->(A:34)":104,"(T:02)->(C:34)":98},"score":2079,"score_norm":0.973,"seq_a_single":35,"seq_ab_match":71,"seq_b_single":35}
ccacgtcgtacgcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatcgacgtcagtgg
+
CCCCCCCCCCCCCDCCCCCCCCCCCCCC@CCCCCCDDEcb^VcZJYTOKSMGLVO[X[]Z_[O[aS^b`cabaKSYaD8ACEEEEEEEEAEEEEEEELEEEEELEEFEBCCCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:2440#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:34)->(A:08)":12},"score":5127,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
cccagcgtagaccattgagtctctgcacctatccttttttattataactttctgaatccttggttgttttcagaaaacaggatttggctcaggattgcccgagcatctggg
+
CCC[d]]a[`TFZ_Z[ebceeeaaee_cdace[dX]deefedddee`edeeeeaaeecadeedceeeeed[\_aacccEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8704:17527#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5410,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccatcacgtcggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggctcagtcggg
+
CCCCCCEEEEEecec^a_bdededeeebededeeeccdeeeddeeeeedaeeeeeeaedceeecebecedeceaeaedebececcEEEEEEEEEEEEEEEDEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8630:10236#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":101,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":97},"score":4849,"score_norm":0.99,"seq_a_single":7,"seq_ab_match":100,"seq_b_single":7}
ccgatctcccgacgtctagggcaatcctgagccaaatcctgttttctgaaaacaaacaaaggttcagaaaaaaaggataggtgcagagactcaatggtagacgtcgggagatcga
+
3@>?4?;Ma^[Y\cbeZeaeTeceaYdebdeeeeeeeeebecefeed_`deebcbdd_[[ccbeaadeeeedd_dWeDEEEEEEEEEEEEEEEEEELEEEEEEEEEEE#######
@HELIUM_000100422_612GNAAXX:7:1:8619:16368#0/1 {"ali_dir":"right","ali_length":71,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":71,"pairing_fast_score":0.941,"pairing_mismatches":{"(C:16)->(T:34)":104},"score":4999,"score_norm":0.986,"seq_a_single":37,"seq_ab_match":70,"seq_b_single":37}
atctacactctttccctacacgacgctcttccgatctccacacagcaggggcaatcctgagccaaggcacacagagataggtgcagagactcaatggctgctgtgtggagatcggaagagcggttcagcaggaatgcggagaccg
+
#####AAA-<)><69:5:>6926AABBAC?:CC;ACBeeeeeeefebeeeaeedeeefeeeeeedeeeeeeeeedeeeaebbebeeeeeee[ecddebbb_eZD][]c@CBA@A@?@CC9C@BBC=;=:+?##############
@HELIUM_000100422_612GNAAXX:7:1:8658:18129#0/1 {"ali_dir":"right","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":74,"pairing_fast_score":1,"score":5333,"score_norm":1,"seq_a_single":34,"seq_ab_match":74,"seq_b_single":34}
tacactctttccctacacgacgctcttccgatctccacgacacacgggcaatcctgagccaaatcacgttttccgaaaacaaaaagggataggtgcagagactcaatgagatcggaagagcggttcagcaggaatgccgaga
+
9;;B@8B@,::8;/6>?;<>@CCCC?C@A;6AA?befeadeeeeeeeeebeedeaeddeefeeeebedeeeeeedddddeddddbddedebecbc``^cce_ee\aY^:?<9;;>?=??#######################
@HELIUM_000100422_612GNAAXX:7:1:8643:15054#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":107,"pairing_fast_score":0.923,"pairing_mismatches":{"(G:34)->(A:07)":12,"(T:02)->(G:34)":98},"score":5259,"score_norm":0.981,"seq_a_single":1,"seq_ab_match":105,"seq_b_single":1}
cccagagcgatgggcaatcctgagccaaatcctggtttacgcgaacaaaccagagttcagaaagcgagaaaaaagggataggtgcagagactcaatggatcgctctggg
+
C_ea[a[^XYZGZL_Z_aGb_eccadTad_aac`_ebecbefeddeedeeedebd_ccbdfeeed[[c__ecd_deddbcEEEEEEEEEEEEEEEEELEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8661:20733#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":102,"pairing_fast_score":0.848,"pairing_mismatches":{"(A:34)->(G:02)":16,"(C:02)->(A:31)":100,"(C:02)->(A:32)":74,"(T:02)->(G:31)":103},"score":506,"score_norm":0.961,"seq_a_single":6,"seq_ab_match":98,"seq_b_single":6}
ccacgtcgtacgggcaatcctgagccaaatccgtgttttgagagaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggtacgacgtgg
+
CCCCCCEE<E@BBDDLEEDEECEEEEEEBEEEEEDA@A999?@ACEEEEEIY]OOUTYRZ4:/0B>>B>=;@8J<8BBBB=BBBBBBBBC7C?BCC5BCIBBIBB7BB@@@@@@
@HELIUM_000100422_612GNAAXX:7:1:8695:7511#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":106,"pairing_fast_score":0.913,"pairing_mismatches":{"(A:34)->(G:17)":30,"(C:02)->(A:34)":94,"(T:02)->(G:34)":108},"score":5030,"score_norm":0.972,"seq_a_single":2,"seq_ab_match":103,"seq_b_single":2}
ctccagacatcgcgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcctagaaggtgaaaaaaggataggtgcagagactcaatgggcgatgtctggag
+
##EeZeec[b_ee[e[eeebdeeeddeeeDee_cac^_fee_dddeedcdddeebdeafeUe^^^UWaacaacceZbEEEEEEEEEEEEEEEELEEEEEEEEEEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:9055:9325#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (accaatc:tgtaaca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"accaatc","obimultiplex_forward_tag":"accaatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaaca","obimultiplex_reverse_tag":"tgtaaca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":933,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCCCCCCCCCB=?^]\\a_cccZbcVZ9A;<EDEEEEEBEEEBE?BEEAEEABEEEEEEEEEEEEEEEEELEEDLCCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8678:7148#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":5739,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacacgacgctcttccgatctcccactgctgtccattgagtctctgcacctatcctttgttttttgaaagcgggagttggctcaggattgcccacagcagtgggagatcggaagagcggttcagaagaa
+
?@C==C?CA;@A=CCC*CCCCCBB7cdbaeebeccX_cd]ceaeeeeeeeedeedeeeddddeeedddddeeefdeeecec`eeeebecMdcTcde\cccQRRRReae=C7:><5B)AAB6?###########
@HELIUM_000100422_612GNAAXX:7:1:8613:2664#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":104,"pairing_fast_score":0.861,"pairing_mismatches":{"(C:02)->(T:27)":107,"(C:34)->(G:02)":25,"(G:34)->(C:02)":12},"score":3237,"score_norm":0.952,"seq_a_single":4,"seq_ab_match":99,"seq_b_single":4}
ccacgacagcagggcaatcctgagccaaatcccttttttgaaaaacaagtggttctcaaactagaacccaaaggaaaaggataggtgcagagactcaatggtgctgtcgtgg
+
CCCCLEEEEEELEELEEEEEEEEELEEbcJJ[eX`eee]X\S^ab`Xb_^_T`O^b`Zb`\O\[YXb\ccZ_dWdbebeEEEEEEEEEEEEEEEEEEEEEEEEAEAEDB?BB
@HELIUM_000100422_612GNAAXX:7:1:9175:14256#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgcgaa:gcgcgaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgcgaa","obimultiplex_forward_tag":"gcgcgaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcgaa","obimultiplex_reverse_tag":"gcgcgaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(a:02)->(g:34)":77,"(c:02)->(a:32)":69,"(c:09)->(a:22)":46,"(t:29)->(a:07)":35},"score":1881,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCCCCCCELBdb``bVZRZURTBOYZCSZL=NN:NCMNGQV_TXaa`_`\BEEEDEJE?ECDEDLEEAECCC@BC<CCBCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8628:8664#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(C:02)->(G:33)":84,"(C:02)->(T:33)":104},"score":1797,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
gtgtccagatgcgacccattgagtctctgcacctatcctttttttattttcactttctgaacttttttttgtttcaggatttggctcaggattgcccgtcgcatctggagac
+
####EEEDEEEEEEEEEEEEEEDEEEEEEEEEEDEEEEEEEEBEa\aUWUXQR[R]]^V]UUX\W[[SJa_^aaDDDDDDDDDKDDDDDDDDDDDDD@DDDD@KCDDD####
@HELIUM_000100422_612GNAAXX:7:1:8663:6588#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":105},"score":6194,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
cccacagcacagggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtgtgctgtggg
+
CCCCCCCCcbe^ecee_][_beecdbbceb]cedeaeSedcbbdde]\[\Xaebee`dee]cc]_b_dcceaeec]e_eea[aZaa][Xb__Uaae_ZbEEEEELEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8870:9658#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agtcgtc:aacgagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtcgtc","obimultiplex_forward_tag":"agtcgtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacgagc","obimultiplex_reverse_tag":"aacgagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(a:02)->(g:34)":76},"score":920,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCBCCDCCCCCCCEEEEEAEEKEEEEEDDE@DC@CC>>@=A8A???QUGJQKK_VSUa\`cb`DBBEEEELFEEEEAC@CACCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9184:14161#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3919,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
tatacggcgaccaccgagatctacactctttccctacacgacgctcttccgatctcctggtaagtagaacaggctcctctagcatagtggggtatctaagtaccgtggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgcggtcttc
+
####CA?A25,;1:9>>*=?=8>C>@CCC@@BAA54A=C1=CCCCCCCCCCDCCCeeefeeeeeeeeefeeeeeeeeeeeeeeeeeeeeedeeefefeeebeeeeceeCCC??BCC@CCCCC@CC====AB>B?B00000><?,?##################
@HELIUM_000100422_612GNAAXX:7:1:8687:19492#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":64,"pairing_fast_overlap":84,"pairing_fast_score":0.79,"pairing_mismatches":{"(A:02)->(T:34)":102,"(C:02)->(T:34)":106,"(G:02)->(T:34)":99,"(G:34)->(C:09)":34},"score":3734,"score_norm":0.94,"seq_a_single":24,"seq_ab_match":79,"seq_b_single":24}
ccccacaccaccctcctccgatctccgatctacggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttggtagatcgagatcgaaggagcgtttcacagga
+
######################?7YZVR[___RF]SY[[[]aK[SZS\]JYVXeeeeee]c_eed]`be_c_ccdcaecbeeZ^a\aEEEEEDECLEELEELEEELFE########################
@HELIUM_000100422_612GNAAXX:7:1:8658:11145#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":107,"pairing_fast_score":0.904,"pairing_mismatches":{"(A:02)->(G:34)":108,"(G:34)->(A:02)":20,"(G:34)->(C:02)":7,"(T:34)->(G:02)":8},"score":3792,"score_norm":0.963,"seq_a_single":1,"seq_ab_match":103,"seq_b_single":1}
tccagcgtcatgccattgagtctctgcacctatccccttcctatgctaggagaagaaaccattgtctccacgaaccggatttggctcaggattgacccatgacgctgga
+
#EEEEELLEEEEEEDEEEELEEEaUU`]\b__aacddXeTf`e]e_aeYeeedbeeee]bde_e]dcaLc_QRRSR^W^]][aEEEEEEEEFEEBEEEEEEEEEEEEL#
@HELIUM_000100422_612GNAAXX:7:1:8699:8904#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(C:34)->(G:02)":31,"(G:33)->(T:12)":46},"score":2510,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
cccatcgctcagatatccgttgccgagagtcgtgtggattaaatagctttgcagcaaggggagcggccagcaagccagtcgtgtccccaagctaggcacagtgttccttgacgccttcggtgaacgatggg
+
CCBDCCCCCCCCCCCCCCCCCCCEEEFEEELFEEEEEEEEEEEaRDYW\bbHRXQRRH`X^WLac^_eb^[a[^]SccUZYZYOZcc``EDEEEEEEEEEFEEEEEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8653:11697#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":93,"pairing_fast_score":1,"score":5542,"score_norm":1,"seq_a_single":15,"seq_ab_match":93,"seq_b_single":15}
gcgctcttccgatctccacgtagtcggggcaatcctgagccaaatcctattttctgaaaacaaagtgaaaagggggataggtgcagagactcaatggcgtacacgtggagatcggaagagcgg
+
##A>A8A@)0,)(;@Oca_]M__Zeeeeca]aa`ccbdcW\Wb^eZceeeeeededddeeebdbdbeeeeeebeeeeeecOb`^]]^]^]___`]]bEEEEEEEEEEE###############
@HELIUM_000100422_612GNAAXX:7:1:8614:3595#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(G:34)":87},"score":4761,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
cccgactagcaccattgagtctctgcacctatccttttttattttcgctttctgaacctttgcttgttttcggaaaacgtgatttggctcaggattgcccgagcgtcatgg
+
CCCEEEEEEabQV[XVaabde`c`^c```ccdZ]_Z_ddeddddccceeeedaedceceeefbbefeeed[YaaaeedEEEEEEEELEEEEEEFEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:9342:5469#0/1_sub[75..82] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gccggcg:gccggcg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gccggcg","obimultiplex_forward_tag":"gccggcg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccggcg","obimultiplex_reverse_tag":"gccggcg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4435,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cctatcag
+
edfeeeee
@HELIUM_000100422_612GNAAXX:7:1:8679:8495#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":89,"pairing_fast_score":0.953,"pairing_mismatches":{"(A:02)->(C:33)":96},"score":3549,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
cctgtacagattcggttggggcgaccatggaacaaatcatccattcctaataaaactcataagttaacaaaagaccctaaattatagatctaaagcaaagctaccttagggataacagctgtacagg
+
CCCCCCCCCCCCCCCCCCCEEEEEEEEEbc_`QQRRJ\\^X[daa__dbcefee`cdfdecebbbbcccced``a^a]^]aaEEEEEEEEEEEEEKEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9229:14517#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atctctc:atctctc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctctc","obimultiplex_forward_tag":"atctctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctctc","obimultiplex_reverse_tag":"atctctc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(G:26)":56},"score":1686,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCBCCCCCCCCCCCCCC@Cc[_Rced_Yccbcc]GX_^LZYZ^caaCDBEEDEBEDEDEEE=EEEEEEEEEEFEEELEEEDCCCCCCCCCCCCC;CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8680:18878#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":93,"pairing_fast_score":1,"score":5505,"score_norm":1,"seq_a_single":15,"seq_ab_match":93,"seq_b_single":15}
acgctcttccgatctccacagcgacagggcaatcctgagccaaatcctattttccgaaaacaaagtgaaaagggggataggtgcagagactcaatgggcagtacgtggagatcggaagagcgg
+
=A+C9C?C?A:=8CBdedcKcc_bdeee_eZdd^eb_ebbedeeeXdeeeeeeeeddbeeebddd_a___eeceeedeec\ecb]\^^^Y_]_cDEEEEEEEEEEEEE###############
@HELIUM_000100422_612GNAAXX:7:1:8666:4114#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":92,"pairing_fast_score":0.73,"pairing_mismatches":{"(A:02)->(C:26)":98,"(A:02)->(C:30)":90,"(A:02)->(T:10)":73,"(G:34)->(A:02)":29,"(T:02)->(C:29)":102,"(T:34)->(C:02)":42},"score":571,"score_norm":0.935,"seq_a_single":16,"seq_ab_match":86,"seq_b_single":16}
ccgcctgtaattcggttggggcgaccaaggacaattcatccttaaataaacagatctattaatcacacaatgtccctttctaagatcaccaataaagctaccttagggataacagtacaggcgg
+
CCCCCCCCCBCCCBCCEEEEEEEEEEEELDEEDEEEDFEFELEEEEEDDDD`a_[WZQJONNM13:19;>5>4;CCCC<AA>A=>7AA>H>DDA@:;D@8@G;;DD?>B?????7<???AAAAA
@HELIUM_000100422_612GNAAXX:7:1:8662:3496#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":6149,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccaactattggtagtactctggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaaatagttggagatcggaggagcggttc
+
;:4699:9???==;;<?;X_d__`eeeee``dc_cecbedededecddeeeeeddeeeeeeeeacbeeeeeedbceb`]b^a]_c__aeeee^a\]^]acacTPRRRRCC;?##############
@HELIUM_000100422_612GNAAXX:7:1:8704:13938#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ggttatt:ggttatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggttatt","obimultiplex_forward_tag":"ggttatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggttatt","obimultiplex_reverse_tag":"ggttatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":2631,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCCCCCCCCCCCCEDEEEEEEDc^\^[]bO[]YbbaebbbeQNPGMRY\[^^acX[_ba_Xb^`aEEEEBEEBEECCDCCCCCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8667:4917#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":6376,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
ccacagcgacagggcaatcctgagccaaatccgtgttttgagaaaaccaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgcagatgtgg
+
BCBCCC_cc`_X[\ceee^ecb_e_edecbd]dedeeec`eeecbedcceccdbccdeebeebZbeecebdbdWd]`^^^eeedbXYYZYSZZ[Y_`ZUcEEEEEEDECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8669:11914#0/1 {"ali_dir":"right","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":105,"pairing_fast_score":0.824,"pairing_mismatches":{"(A:34)->(G:02)":20,"(C:02)->(A:34)":89,"(G:34)->(A:02)":22,"(T:02)->(C:34)":98},"score":4010,"score_norm":0.952,"seq_a_single":3,"seq_ab_match":100,"seq_b_single":3}
tctccacagtcgagggatgacgccaaggaacacgaacggagaggagcacccctcctcacgagacaaccaatatcgacgactctcggcaacggatatcctcgactgtggaga
+
###EEEEEEEEEELEBEEELELEeKe_ecca[c]bbb\ac`cTccbcKY^a\Xac``W`a_dc[c_\aa_a`_YU\ZU^[^__\\SaELEEEEEEEELEEEEEEEEEE###
@HELIUM_000100422_612GNAAXX:7:1:8613:8579#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":73,"pairing_fast_overlap":86,"pairing_fast_score":0.88,"pairing_mismatches":{"(C:02)->(T:34)":104,"(G:02)->(T:34)":108,"(G:34)->(T:02)":34,"(T:33)->(G:02)":35},"score":1832,"score_norm":0.953,"seq_a_single":22,"seq_ab_match":82,"seq_b_single":22}
cccactagcgtgatatccgttgccgagagtcgtgtggattaaatagcattgcaacacgggggcggctagcaagccagccgcgtccccgagttaggcacagtgttccttgacgccttcggacgctagtggg
+
CCCCCCCCCCCCCCCCCCCCCCEEEEEEEBEFELKEEc_Xede^dbcde[aXcRbc_a`__abd?DCEBEDDEEDEEDEEDFEEEEEEEEEEEEEEEEBEEEELEEELCCDCCCCCBCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8611:14767#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4982,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacagtcgaggggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggctcgactgtgg
+
CCCEEEEcc]`[b]`Z`_LX`ebe\e]`___cZb__cc^eY__cebbddaeeVdeYdbbbeeedbeaadea_ccccceccDEEEEEEEEEEEEEECEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8674:6317#0/1 {"ali_dir":"left","ali_length":67,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":60,"pairing_fast_overlap":67,"pairing_fast_score":0.938,"pairing_mismatches":{"(G:31)->(T:09)":52},"score":1334,"score_norm":0.985,"seq_a_single":41,"seq_ab_match":66,"seq_b_single":41}
ccacactctgcgatatccgttgccgagagtcgttatgttcacgaaagatgcggctactaccccccatagattcactcgcgagaactgaggggcacgcttttcttctcttgtttgagttcctggacgcgattcgcgtcggcagagtgtgg
+
CCCCCCCCACCCCCCACCCCCCCCC@CCCCC>C@?:@??@Cc\da[Z<SWVCOZZOO?T_^\_WP\39>2-/1/.2949C<<B9@DDAAAEAEE>>/;7EEE5EEEEEC<?CCC/?>??A>3?7C@?C@CCB?CBCBCCCCC?C@CCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:14454#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":5646,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
ccacgatcgcgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccactgcagt
+
CCCCEEEbe_e^a[\b_d[`c\e_aecTXZYU^Z\^_aZd\ca\`a`eee_eeec`eedddcddddeedeedded_dccd_`\X`ccccbEEEEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:5807#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":3154,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcccacacccagatctacactctttccccacacgaccctcttccgatctccttcgccacaccaactctttcattcagacgtatacgtatgtatatacggtggcgaaggagatcggaagagcgttcagcaggaatgcggagatcgagatcgaatgctg
+
###############################@?+6?.?4==A6?8A??AZXZcSVRQXSRYOYcba[`ecdbbdccda_`]\abcc`bbcbc`c`ZQaEEEEEFDEEE#################################################
@HELIUM_000100422_612GNAAXX:7:1:8698:8988#0/1 {"ali_dir":"left","ali_length":66,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":48,"pairing_fast_overlap":66,"pairing_fast_score":0.762,"pairing_mismatches":{"(A:34)->(G:02)":49,"(C:02)->(T:28)":95,"(T:11)->(G:02)":61,"(T:34)->(C:02)":46},"score":400,"score_norm":0.939,"seq_a_single":42,"seq_ab_match":62,"seq_b_single":42}
cccgacacacaccgaaggcgtcaaggaacacctaacgaattggcattcaccccccccgcctccgagatacgttgcgcggtcggggtgcgcgtcgtattctgtattctatatgtcaaaacgactctcggcaacggatatctgtgtgtcggg
+
CCCCCCCCCCCCCCCCC2BCCCCCCCCCCCCCCCCCCCCCBB?EELAELEEEDDDD;4?;5C;C@002.1>XOXXYY`9:EAEBCEEEEEEEEEFEEEEDEEEEEEEECCCCCCBCCCCCBCBCCCCCCCCCCCCCCCCCCCCCBCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8665:15212#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":103,"pairing_fast_score":0.82,"pairing_mismatches":{"(A:02)->(G:32)":108,"(A:02)->(G:34)":86,"(A:24)->(C:02)":15,"(C:02)->(A:32)":79,"(C:32)->(T:02)":6,"(T:02)->(G:34)":102},"score":813,"score_norm":0.942,"seq_a_single":5,"seq_ab_match":97,"seq_b_single":5}
ccagtcgtcacggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggtgacgactgg
+
AAAAAJBCCB5;.+B+2222C>3@<@,@,@^X^[OWN^VBCITI_`=BEEEEEEEEBECBEEE?EEEEDEBEEEEEEEJEEEEEELEEEEEDEEEEEEAEELEEEECJACCCC
@HELIUM_000100422_612GNAAXX:7:1:8690:21182#0/1 {"ali_dir":"right","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":103,"pairing_fast_score":0.91,"pairing_mismatches":{"(A:02)->(G:34)":91,"(A:34)->(N:04)":32,"(C:34)->(N:04)":31,"(G:34)->(N:04)":30},"score":4462,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
catctccagcgagatcccattgagtctctgcacctatcctttttttcgttttctgaatctttgtttggaaaaaaacaggatttggctcaggattgcccgatctcgctgagatc
+
#A8ACbceV^X[RZWXWY]OYU]RQQQRQIIIN[[]ZVd`eeeeebdfe_fceedeccddedededdacddefdb]^aTcEBEEFEDEEELEEEBEEEEEEEEEEEEE#####
@HELIUM_000100422_612GNAAXX:7:1:8836:17806#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcgcca:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcgcca","obimultiplex_forward_tag":"ttcgcca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"score":1806,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatagttctataacaaaacaattcgccagagaactactagcaacagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCBCCCCBCCCCCCCCCZWWa\Wc_Se_Nee`c^]Z[ZXYWYc\a\[@=8DAABEEEFEBFEEEEEEEEEEEEELEEELECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:9515#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":4024,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
ccgacgcagtgggcaatctgagccaactccttttttcaaaagaaaaaaaaaagaagggttcagaaagcaagaaaaaataaaggataggtgcagagactcaatggactgcgtcggg
+
BBBBBBB`c`PRXLSM^\^SaZOY[abab^dddda\RW\Z_I]\\\\Z\_\__RV[ZZYZU``]_d`W]_^[]aBEEEEEEEEDEEEEEEEEECEEEEEEEEEEEEEECCCCCBC
@HELIUM_000100422_612GNAAXX:7:1:8685:20051#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":40,"pairing_fast_overlap":92,"pairing_fast_score":0.449,"pairing_mismatches":{"(A:02)->(C:29)":107,"(A:02)->(C:34)":96,"(A:02)->(G:23)":85,"(A:02)->(T:34)":91,"(A:18)->(G:02)":54,"(A:34)->(C:02)":46,"(C:02)->(G:27)":100,"(C:23)->(T:02)":53,"(C:34)->(A:02)":17,"(G:02)->(A:11)":80,"(G:30)->(A:02)":55,"(G:33)->(T:02)":50,"(G:34)->(C:02)":24,"(G:34)->(T:02)":38,"(T:02)->(A:31)":93,"(T:26)->(G:02)":25,"(T:34)->(A:02)":37},"score":555,"score_norm":0.804,"seq_a_single":16,"seq_ab_match":74,"seq_b_single":16}
ccatgcagactgttatccctaaggtagctttattgatgatcttttaaggggtcagtaaatgataaatatatctatatagatgaggatgattaatccacggtcgccccaaccgaattctgcatgg
+
CCCCCCCCBCCCCCBCLEEEEEELDDK?DDEEECEELLEEBEEEDLEEEKA?A<H8:85922211aI^^bP]aeY[\[>5<>::AA.:A5LEIEEL7BEE==<>?EGACBCCCCC=CBCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8680:1630#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":89,"pairing_fast_score":0.988,"pairing_mismatches":{"(T:02)->(G:34)":108},"score":4434,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
cacgacctccttccgatccccaaccgcctcaagaacataactaatactactcaggcacaaaaattaacatcccaaaataatagggtatctaatcctagtggcggttgggggtggggactgcgggtca
+
###################EEEEEa^^]aeeeeeedeeeededeeccce__cdddddeeceebddeeeecbedddedeeKY`_W_\cEEEEEEEEEEEEEEEEEEEEL###################
@HELIUM_000100422_612GNAAXX:7:1:8693:12796#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":85,"pairing_fast_score":0.805,"pairing_mismatches":{"(A:02)->(C:34)":96,"(C:02)->(T:34)":94,"(C:34)->(G:07)":66,"(G:32)->(T:06)":40,"(G:34)->(A:02)":25},"score":3084,"score_norm":0.941,"seq_a_single":23,"seq_ab_match":80,"seq_b_single":23}
ccagcatcgagccgaaggcgtcaaggaacactgtgcctagctcggggacacggttggcttgctggccgccccccgtgctgcaaagctatttaatccacacgactctcggcaacggatatcctcgatgctgg
+
CCCCCCCCCCCCCCCCCCCCCCCELEdca]`VWQWRUW\E\T[P_c^X`Z^S[WMYEKSGWPNKOGbbee\dbbbdbd_TcccEDEDEEDEEFLELEEEEEEDEEEEECCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8669:4780#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":103,"pairing_fast_score":1,"score":4514,"score_norm":1,"seq_a_single":5,"seq_ab_match":103,"seq_b_single":5}
cccacactctggggcaatcctaagccaaatccgtgttttgagaaaacaaggggttctcgaactagaatccaaaggaaaaggataggtgcagagactcaatggcagagtgtggg
+
CCCCCYZ[[[[UZY_Wa]][\OaaLece\^^U\YVVZUaccYYdeeeedaee^_efedefeacceeeb`beeceaEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8632:3292#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":85,"pairing_fast_score":0.854,"pairing_mismatches":{"(A:20)->(C:34)":96,"(A:34)->(C:06)":27,"(G:31)->(T:10)":56,"(G:34)->(A:02)":25},"score":4967,"score_norm":0.953,"seq_a_single":23,"seq_ab_match":81,"seq_b_single":23}
ccacgacacacccgaaggcgtcaaggaacactgtgcctaacccggggacgtggctggcttgctggctgcaccctgggttgcaatgctatataatccacacgactctcggcaacggatatcacgctagtggg
+
CCCCCCCCCCCCCCCCCCCDCCCELaG`___```_Sa_M[_cdac`_\a[cTVVXCZ[[\\ZYUXVcee_ebeedbcb\deaaaac_^e`a^LXaD[Va^da_cccXdCCCCCCCCCCCCCCDCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8652:19677#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":4741,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
ccagacacgctgggcaatcctgagccaaatccttctttctgaaaacaaataaaagttcagaaagttaaaatcaaaaagggataggtgcagagactcaatggcgtagcgctgg
+
CDCCEEEEEEcOc^aa`bH_\_beMbeecc_e\\\a^c^_KVbedddcdb_e``edbddeeeeebcceebdbbddeeeaeEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8697:17277#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4165,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctacactctttccctgcacggcgctcttccgatctccgaggcttcaccaactctttcattcagacgtatacgtatgtatatacggaagcctcggagatcggaagagcggttcagcaggaatgcggagaccgatatcgtatgcg
+
#######################################AC@AAA,A>;eeee``a_^_\cc]eecceeeceddeaeeeebeecceeaeeeeedddadWeccR]ebe^:?9>+?8?3?>AA=AA34=?#############################
@HELIUM_000100422_612GNAAXX:7:1:8664:19697#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":105,"pairing_fast_score":0.931,"pairing_mismatches":{"(A:02)->(C:34)":103,"(T:02)->(G:34)":100},"score":5646,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccacgtacgctgggcaatcctgagccaaatcacgttttccgaaaacaaacaaagattcagaaagcgaaaatcaaaaaggataggtgcagagactcaatggagcgtacgtgg
+
CCC_Te`c`^cJa^^WYTHVYba]\e_`c]cc`Oabeeb]_[deed_bdaeeadeee`edfeeeb_acdce`ceeefeXeRQQJRZXXXZaaEEEEEEELEELEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8633:18969#0/1 {"ali_dir":"right","ali_length":35,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":32,"pairing_fast_overlap":35,"pairing_fast_score":1,"score":2571,"score_norm":1,"seq_a_single":73,"seq_ab_match":35,"seq_b_single":73}
tatgcttacttttttaatgatacggcgaccccccagatctacactctttccctacacgacgctcttccgatctccagtgcaaattcggttggggcgaccccattgtggagatcggaagagcggttcagcaggaatgccgagaccgatatcgtatgccgtctcctgtctgaaaacaatatgc
+
######################################AA/A<=AAA?CCCBBAAABB:ABB<CCCCCCCCCCeeeed`eeeeeeeeeeeeeeeeeeceeedeeebebCCC?CCACCCCCCCCBC???>CCBC=A8A############################################
@HELIUM_000100422_612GNAAXX:7:1:8610:3520#0/1 {"ali_dir":"left","ali_length":64,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":64,"pairing_fast_score":0.869,"pairing_mismatches":{"(T:02)->(A:34)":94},"score":1310,"score_norm":0.969,"seq_a_single":44,"seq_ab_match":62,"seq_b_single":44}
gatatcccccaacataccaaaactagctgtcactccaccagttgtcggagatgtaaggattgatacataaaataactttttatttaattgataatcatataaagcagacgatattttagccatttgcatcaagctcaaacttccttcctgca
+
CCCCCCCCCCCCCACCCCCCCCCCCCCCCBCCCCCDCCCBAACCCCEDCEBEE?CBE>BC\[_adY\X[X_beaUKRQWVacEEEBLEEDEEBLEECCEEEEEEEEEDCCCCCCCCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8623:1161#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(G:34)->(T:13)":26},"score":4880,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccttcctggttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccccaggaaggagatcggacgagcgattc
+
###@@C?A=9=6?+,AA=RRQRQ\\EaUceccec]eaeWa_c\Zdaeecfee`eeedeeeeeaeeedeeeedceefee]]SdcYa__dcccbEEFEEEEEEFEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8673:6901#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":103,"pairing_fast_score":1,"score":5084,"score_norm":1,"seq_a_single":5,"seq_ab_match":103,"seq_b_single":5}
ccagatcgcgaccattgagtctctgcacctatccttttcctttggattctagttcgagaaccacttgttttctcaaaacacggatttggctcaggattgccctcgcgatctgg
+
CCCCCEEEcX_Tca_e^aRcada_ccc`eZebZeaece_ecbbeedeeefffeedceeedeeca^debbeddee^eeeeeEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8677:17215#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":100,"pairing_fast_score":0.938,"pairing_mismatches":{"(G:34)->(C:02)":15,"(T:02)->(G:34)":107},"score":4519,"score_norm":0.98,"seq_a_single":8,"seq_ab_match":98,"seq_b_single":8}
tccgatctcccgacgtctaccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtaacccaggatttggctcaggattgccccgagtactgggagatcgaa
+
########EEEEEELEEEEEEBEEEEc_caa`XbZ^dbceb[e_^edccbda_edeeaeaefe`cSa^c^a`^_S_a`ZYTT[cde]URARRLaa]cEEEEEEEEELE########
@HELIUM_000100422_612GNAAXX:7:1:8617:11593#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":102,"pairing_fast_score":0.667,"pairing_mismatches":{"(A:02)->(G:32)":76,"(A:02)->(G:33)":87,"(C:02)->(A:29)":100,"(C:02)->(G:30)":82,"(C:14)->(G:02)":25,"(G:02)->(C:17)":107,"(G:14)->(T:02)":24,"(T:02)->(G:25)":53,"(T:02)->(G:31)":103,"(T:15)->(C:02)":21},"score":766,"score_norm":0.902,"seq_a_single":6,"seq_ab_match":92,"seq_b_single":6}
ccactagctcggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgactacgtgg
+
BBB?BBDADD=:9=;<:;-=92288AA@@b[V__KT`SOGFA>FNQTI9:;@C@<8@2/D;D@9=<45>19)@@DJDDDDDH>5D1KDDDB>>@>??<5GB@IB@7;=:B>BBB
@HELIUM_000100422_612GNAAXX:7:1:8647:3107#0/1 {"ali_dir":"right","ali_length":65,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":65,"pairing_fast_score":1,"score":4782,"score_norm":1,"seq_a_single":43,"seq_ab_match":65,"seq_b_single":43}
accgagatctacactctttccctacacgacgctcttccgatctcctaatcatattcggttggggcgaccaagcctcggaagctaccttagggataacagatgattaggagatcggaagagcggttcagcaggaatgccgagaccgatatcg
+
:?A.?@?;;:);*5/:):;4>?'AAAAA>?AC@C@CCCABCCCeeeeedeeeeeeefebeeeeeeeceeeeedeeeeeeeefeeeeeeedeeeefeedefdafcbcc`CCB@=67969BAB@??<3:?#######################
@HELIUM_000100422_612GNAAXX:7:1:8616:1312#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":102,"pairing_fast_score":1,"score":5655,"score_norm":1,"seq_a_single":6,"seq_ab_match":102,"seq_b_single":6}
cccacactagcgggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggctagtgtggg
+
CCCCCCecacXZ`Z]NXaRececTcbOecTceeeeefeeeedXeeedfbdcbedeeee_feee]ceeedddeb_ededea`Zc`Z^[[aEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8675:21039#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":89,"pairing_fast_score":0.756,"pairing_mismatches":{"(A:02)->(C:34)":97,"(A:33)->(G:02)":43,"(C:02)->(A:33)":84,"(G:35)->(T:02)":20,"(T:34)->(A:02)":37},"score":1732,"score_norm":0.933,"seq_a_single":19,"seq_ab_match":83,"seq_b_single":19}
ccttcgccaattcggttggggcgaccatggaattcatcatccaccacaaataagattatctaatcactcaaaagatcctaacaatagatcaaagaccaagctaccttagggataacagtggcgaagg
+
CDCCCCCCCCCCCCCCCCCMEEEEEEELEBEEEEECLEEBDAKC_cb`aUTMZaRP\RbP[_bO]bc^_ceeJbE3EEBADEBK@>@E5EEEE>EELDDBDA9AA8>E>CCCCC@CCCCBCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9205:20589#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agcagag:agcagag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agcagag","obimultiplex_forward_tag":"agcagag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agcagag","obimultiplex_reverse_tag":"agcagag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(A:02)->(C:28)":54},"score":1720,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCDCCCCCCCCCCd]ad[edd\d`^XT[XZ`OU]Y]`]`bD@=AA=CF+CC;EDFDEEEEEEEEEEEEEELEEEECCCCCCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8798:6090#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:atgatca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atgatca","obimultiplex_reverse_tag":"atgatca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(c:02)->(t:34)":81},"score":748,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCACBLCEEEEEEEEFEE@@AEBCDD=@CABC@;?AC@CECEAC_VT]_^U][^cZEEEEEEEEELCCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8683:11264#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gataggc:gataggc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gataggc","obimultiplex_forward_tag":"gataggc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gataggc","obimultiplex_reverse_tag":"gataggc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":3587,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCDCCCCCCC@CEEEMaaa\[ee_e``O^aZ_\c^a_]^a[RS\ZMceb``Z^a]]XXX`[eeT]cd\dT`^[QCCCCCCCCCCCCC@CCCBC
@HELIUM_000100422_612GNAAXX:7:1:8647:2431#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":4022,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
atcttgggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggacgcacgatggagat
+
####EEEEEEEEEEEEEEadbdeabaebeab\bde`\fceeebdceeedeeebeaea__eececaac`cZddccEEEEEEEEEEEEEEEEEEEEFEEEEEEEEEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8619:12377#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":85,"pairing_fast_score":0.951,"pairing_mismatches":{"(G:34)->(T:14)":31},"score":3847,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
cctacacgacgctcttccgatctccgcgatgacaagaagaccctatagagctttatcatattcatattacaaatatgctaagattcggttggggcgacctcatcgcggagatcgaaggagcggttcagcag
+
#########A@AA=3=<?,8A@AWZ[ZXRREOOI]P\[eeeb_efeeedd`edeede_eeeebceeaddedeceeddU\\Ua\cEEEEEEEEEEEEEEEEEEEDEEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:8655:3238#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":85,"pairing_fast_score":0.768,"pairing_mismatches":{"(A:34)->(G:02)":27,"(A:34)->(T:02)":30,"(G:31)->(T:02)":53,"(G:32)->(C:02)":25,"(G:34)->(A:02)":35,"(T:02)->(C:30)":96},"score":164,"score_norm":0.929,"seq_a_single":23,"seq_ab_match":79,"seq_b_single":23}
ccatcgcgagaccgaaggcgtcaaggaacactgtgtctaacccgagggcatggctagcttgctggtcatctcttgtgttgcaatcgtatttaatccacatgactctcggcaacggatatctctcgcgatgg
+
CCCCCCCCBCCCCCBCCCCCC@CEJELEELEEEELEEEEECEEBCEECEEE?IA<C?8CBCE>E-??CC<AECEECDC@CDYR`A3=A0C>AA3;H;9924C>=DAA=A?9BB88B9B8AA?9????????
@HELIUM_000100422_612GNAAXX:7:1:8675:16045#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":101,"pairing_fast_score":0.959,"pairing_mismatches":{"(C:34)->(G:09)":18},"score":5066,"score_norm":0.99,"seq_a_single":7,"seq_ab_match":100,"seq_b_single":7}
ccatcgcgagagggcatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtctcgcgatgg
+
CCCCCCCdeeccbcdL^F[TdS_\ae_e_\dcdcdXZUMTROaccdddcddceeebeee`^cd_beedee_ccc\cTccc\d]_dEEEEEEEEEEEEEEEEEEEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8670:10065#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":3223,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgcacaacgagatctacactctttacctacactacgctcttccgatctccaactattccgtatatacatacgtatacgtctgaatgaaagagttggtggctgataggagatcgaaagagcggttcagcaggagtgccgagacggacatcttatgcg
+
##################################@@@B<B<>@9;;6:BS`dUd```]\UQTS\ORRRW[WZ^X_Z\^\\^]`]aZ]^`R[Q]`aZaabaP^AABCDD#################################################
@HELIUM_000100422_612GNAAXX:7:1:8672:19510#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5802,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
tccctcgccgcgctcttccgatctccgaatatccaagaagacccttagagctttatctaaccatatgatatcatattcctagattcggttggggcgaccgatattcggagatcggaagagcggttcagcaga
+
######################BCbeececTTecYcc\ecaedeeeeeeaeeeeeeeeedeeeeefdddeefdedeefecbb^b^Y^[^^^[aac^c__]U^a]`___A=A=;=0?################
@HELIUM_000100422_612GNAAXX:7:1:9039:9123#0/1_sub[22..121] {"ali_dir":"left","ali_length":68,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atgcaga:)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgcaga","obimultiplex_forward_tag":"atgcaga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_primer":"tagaacaggctcctctag","pairing_fast_count":61,"pairing_fast_overlap":68,"pairing_fast_score":0.938,"pairing_mismatches":{},"score":3015,"score_norm":0.985,"seq_a_single":40,"seq_ab_match":67,"seq_b_single":40}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCBCCCCCCCCEEEEDEELa]dccTL]`a[YYHVe^S`d_^_aa[Y]][Z^babcXYd[[b^]__a_EAEEEECEBEDECCCCCCCCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8689:6039#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":89,"pairing_fast_score":0.907,"pairing_mismatches":{"(A:02)->(C:34)":105,"(A:02)->(T:34)":107},"score":2544,"score_norm":0.966,"seq_a_single":19,"seq_ab_match":86,"seq_b_single":19}
ccagaaccgattcggttggggcgaccaaggaaatatcatcctaaataataaagatcaataaatcaaattacagaccctttaattaagatcacaaacaaagctaccttagggataacagcggttctgg
+
CCCCCCCCCCCCCCCC@CCEEEEa[aUbSeec]^]\[\Z_[Z[^][Ncccccef_Z_cc_acbFEEEEEEEFECELEEEEEEEEEEEEEEEDDEEEEEEEEEEELELECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8656:6001#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5137,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
tcctacacgacgctcttccgatctccacagcgacaccattgagtctctgcacctatcttatttttaatagacaataagatttggctcaggattgccctgtcgctgtggagatcggaggagcgctccagcaga
+
########@1>ACA@@CC7878+(][[^_d_ZadTcddbaeecedeebceeeeeeedbc^ebedddaedeced]eebeaaeebbbeabb]`ce``dcEEEEEDDEDEE########################
@HELIUM_000100422_612GNAAXX:7:1:8656:20635#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":97,"pairing_fast_overlap":100,"pairing_fast_score":1,"score":5062,"score_norm":1,"seq_a_single":8,"seq_ab_match":100,"seq_b_single":8}
tccgatctccacgcagacaccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtaacccaggatttggctcaggattgccctcgtacgtgggagatcgga
+
########EEFEEEEEEEEEEDEEEcbe^\]UPec_ebbeceeeeeeebbeeeedeedeeeeededebdceebaaa_a[]^]]c^b^Za_c_a___acEEEEEEEEEE########
@HELIUM_000100422_612GNAAXX:7:1:8652:16412#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":105,"pairing_fast_score":0.873,"pairing_mismatches":{"(A:02)->(C:34)":100,"(C:02)->(G:34)":87,"(G:34)->(C:02)":4,"(G:34)->(T:02)":17},"score":4213,"score_norm":0.962,"seq_a_single":3,"seq_ab_match":101,"seq_b_single":3}
ctcgacgcagtccattgagtctctgcacctatccttttttattataactttctgaatccttggttgttttcagaaaacaggatttggctcaggattgcccactgcgtcggg
+
CBCLEEEEEEEEEEEELcX_dc_]]_]\U[]_cd_b_eeeeaddeebfeeeedfe`e]Seff_d_dcdcSWYU]^eeeccEEEEEELEEEEEFEEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8659:17593#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":102,"pairing_fast_score":0.949,"pairing_mismatches":{"(A:02)->(C:34)":104,"(C:02)->(T:34)":108},"score":4919,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagagacgtgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgccccacgtctctgg
+
CCCCCCSY]Y[SY^ZaUcX[[Z[ZM^\[ad]eeedeeeSeeebedebece``eeffeed`ecedcdeebeebdec^cccdEEEEDEEEEEEEEEEEEEEEEEELEEELCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8694:17297#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":90,"pairing_fast_score":0.851,"pairing_mismatches":{"(A:02)->(G:32)":108,"(A:02)->(G:34)":96,"(T:02)->(G:34)":102},"score":2660,"score_norm":0.956,"seq_a_single":18,"seq_ab_match":86,"seq_b_single":18}
ccagcagcactccattgagtctctgcacctatccttttttgtttaattttaactttctgaacttttacttttatttttttgttttcggaaagcaggatttggctcaggattgcccagtgctgctgg
+
CCCCCCCCCCCCCCCCCCEEEEEEEEEEEEEEEEEEEEece`W\\\_aaa_[_acc]`cU`^bbcdSU^`_^_`_UaaaEEEBEEEELEEEEEEELEEEEELEEEEEJACCCCCC?CCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8639:13291#0/1 {"ali_dir":"right","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":98,"pairing_fast_score":1,"score":6439,"score_norm":1,"seq_a_single":10,"seq_ab_match":98,"seq_b_single":10}
cttccgatctccagagcgagtccattgagtctctgcacctatcaaagcttgtattttcaggattgctatagagtggatgcttggctcaggattgcccactcgctctggcgatcggaag
+
###A@;=B@;a^_eece]_Z_c\cdd]dQNQQOcJb_cdeeebdefedeeecdeeeeddddaeed_ebeec_c\c]cU_Z]^bWRce^eadddeecbbdde`bddcEE##########
@HELIUM_000100422_612GNAAXX:7:1:8682:19861#0/1 {"ali_dir":"right","ali_length":42,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":39,"pairing_fast_overlap":42,"pairing_fast_score":1,"score":3052,"score_norm":1,"seq_a_single":66,"seq_ab_match":42,"seq_b_single":66}
atttttttaatgattcggcgaccaccgagctctacactcttgccctacacgacgctcttccgatcttattttagccatttgcatcaagctcaaacttccttcctgcatagatcggaagagcggttcagcaggaatgccgagaccgatctcgtatgccgtcttctccttgaaaaa
+
###########################################@B=;?CCBCCCBCC?CCCB@C@@eeeeeddbbebbebeeeedeeeeec_ebecdececeeed^eeCCC:>?9<5?=>=A>9A>0/,008A;>?A<AA>@################################
@HELIUM_000100422_612GNAAXX:7:1:9296:3033#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtggact:gtggact)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtggact","obimultiplex_forward_tag":"gtggact","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtggact","obimultiplex_reverse_tag":"gtggact","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(c:09)->(g:34)":77},"score":2736,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCDCCCCCCCCCCCCCCC<CEECELEEEEEEEDDBAebbc_cee_ec`c]Zc_a[Y\^YSN_`_]caJ^\^acbdFdaJcCCCBCCBCCCB?CCCC@CC
@HELIUM_000100422_612GNAAXX:7:1:8616:4287#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(A:02)->(G:34)":86},"score":4397,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccacgcagacaccattgagtctccgcacctatccttttttgattttcagtttctgaacttttatttgtttgttttcggaaagaaggatttggctcaggattgccctgtctgcgtgg
+
CCCCCCCCEEEEEDEEEdbdc_^__c\ceebeaeeea^`]Ueeeeedddfddedebccddecdd`bc`dcddedd]c]cEEDEEELEEEEEEEEEEEEEEEEEEEEEEBCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9294:21161#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acagaca:gcgcgaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acagaca","obimultiplex_forward_tag":"acagaca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcgaa","obimultiplex_reverse_tag":"gcgcgaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(n:04)->(a:31)":52,"(n:04)->(t:28)":51},"score":3010,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaagctgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCCCCCCCDCCCCCCCCCCEEEEDEEEEcJb_[]]Yb^Md]]\W_\[_KKCFPPPMNPca]^dad^ddfebUedWcZ\ec`CDCCCCCCCCCDCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8685:8345#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":87,"pairing_fast_score":0.952,"pairing_mismatches":{"(A:02)->(C:34)":99},"score":4404,"score_norm":0.989,"seq_a_single":21,"seq_ab_match":86,"seq_b_single":21}
cagacgtacggatatccgttgccgagagtcatatttttttctgttggcctcggcagccgacggagccctcgaagggctcgccggtgccttagctttaccgtgttccttggcgtcatcccgtacgtctgg
+
CCCCCCCCCCCCCBCCCCCCCa_becefcRLVIX\WY`\acaUd[`acaOUTGUY^\]]^a_[`U_`ddcbbccbYV]bbbedededRddb^EEEEEELEEEEEEEEECCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8615:1380#0/1 {"ali_dir":"left","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":90,"pairing_fast_score":0.908,"pairing_mismatches":{"(T:02)->(C:34)":101,"(T:02)->(G:34)":105},"score":3368,"score_norm":0.978,"seq_a_single":18,"seq_ab_match":88,"seq_b_single":18}
ccgcgccggctgttatccctaaggtagctttgtttgtgatcttaattaaagggtctgtaatttgatttattaatctttattatttaggatgagatatttccttggtcgccccaaccgaatccggcg
+
CCCCCCCCCCCC@CCCCCEEEEEEDEEEEEEe]edd_^cZcYYSZZbbcePee\e_caN]cbabUcc`bc\`T`a`caaaV[^EEEEEEEEEEEEDEEEELCEELEEECCCCDCCCCCCCCBCBAC
@HELIUM_000100422_612GNAAXX:7:1:8618:4698#0/1 {"ali_dir":"right","ali_length":80,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":73,"pairing_fast_overlap":80,"pairing_fast_score":0.948,"pairing_mismatches":{"(A:34)->(G:08)":39},"score":4999,"score_norm":0.988,"seq_a_single":28,"seq_ab_match":79,"seq_b_single":28}
cgttccctgcgcgacgctcttccgatctccgtgtgggcaatcctgagccaaatccttaagttaaaatcaaaaaaggataggtgcagagactcaatggacagcagtgggagatcggaagagcgcttcagcagaaatg
+
####################A8A748?2aZaad\\M[]FRKJJc^dTdeaTecdaedbeceed]ce]cdeeeba`ece]a`]`[\acaa\cUadbc_cacZUcYXZVZCC@#########################
@HELIUM_000100422_612GNAAXX:7:1:8691:13385#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":73,"pairing_fast_score":0.786,"pairing_mismatches":{"(A:29)->(C:32)":70,"(G:33)->(A:14)":49,"(G:33)->(A:27)":46,"(T:02)->(C:34)":97},"score":1472,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacgtgtacggatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcgtttattgttctccttggcacatgccgtgccgtacacgtgg
+
CCCCCCCCCCCCDCCCCCCCCCCCDCCCCCCBCCCEEEEEEDDEcBYbDK[]]\a[a[N\\WUVT\`\VAY`]dEEDECCA>CCEDEAEEDCEEEELEEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8700:4451#0/1 {"ali_dir":"right","ali_length":70,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":70,"pairing_fast_score":1,"score":5045,"score_norm":1,"seq_a_single":38,"seq_ab_match":70,"seq_b_single":38}
gatctacactctttccctacacgacgctcttccgatctcccagtagctggatatccgttgccgagagtcacagtggggtgttccttgacgccttcggcagctactgggagatcggaagagcggttcagcaggaatgccgagacgga
+
#########AA=>?ACCB(@@C@CBCCCC?CCC8CCCCeeedeeeeeeeeeefeeefedeeeeeeeeeefebeeeeeceeeebdbdccbccdcebdeeYY\\a^]feeCCD<3<<?/?BBAAA#######################
@HELIUM_000100422_612GNAAXX:7:1:9266:17939#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aatgtcc:aatgtcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatgtcc","obimultiplex_forward_tag":"aatgtcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aatgtcc","obimultiplex_reverse_tag":"aatgtcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(a:02)->(t:34)":81},"score":2709,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCCCELDEFEEEED`]^WYJY[W[XdX`_c]aWAPLOHT[[Zcda]c]I_]R^XW^KZcaUUaECLCCCCCCCCCCC@C@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:3916#0/1 {"ali_dir":"left","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":85,"pairing_fast_score":0.793,"pairing_mismatches":{"(C:32)->(G:02)":54,"(C:33)->(A:02)":36,"(C:34)->(G:02)":66,"(G:32)->(T:02)":53,"(G:34)->(A:02)":25,"(T:34)->(C:02)":38},"score":1264,"score_norm":0.929,"seq_a_single":23,"seq_ab_match":79,"seq_b_single":23}
ccagagacagcccgaaggcgtcaaggaacactgtgcctaacacggggacgtggctggcttgctggccgcaccccgtgttgcaatgctatataatccacacgactctcggcaacggatatcgctgtctctgg
+
CCCCCCCCCCCCCCCCCCCCCDCELEEEEDEBEEEKELEEECEEEEEEBED?JJCCE=AEE8CBELEEEEE_]ed^a[_UYXJWY_`\_aabEEBEDDBDD>E=E:EEC@C?CBCC@BBBCCACA=B=?CC
@HELIUM_000100422_612GNAAXX:7:1:9205:16470#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aatgtcc:aatgtcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatgtcc","obimultiplex_forward_tag":"aatgtcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aatgtcc","obimultiplex_reverse_tag":"aatgtcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1281,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCACCCCCCCCCCCEEEEEEEEddd_bbYP@][V]N`^_cccEDEDDE=DECCEEEEEEEEEEEEEEEEEELEEEECCCCCCCCCCCCD@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:1669#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":5288,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ctacacgacgctcttccgatctccacagctgacgggcaatcctgagccaaatccttaagttaaaatcaaaaataggataggtgcagagactcaatgggtcagctgtggagatcggaggagcggtgcagag
+
###?A82=C@C@C@@9:6C=C@_aa____^a`ed^bedcbce`efeeeeeedeeeeefeeeeeebebdeeeeedd_ad_da`c^cdbeabed]N^]bEEEEEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8676:21268#0/1 {"ali_dir":"right","ali_length":79,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":79,"pairing_fast_score":0.816,"pairing_mismatches":{"(A:34)->(C:10)":56,"(A:34)->(N:02)":37,"(C:34)->(N:02)":38,"(G:34)->(N:02)":39,"(T:34)->(N:02)":36},"score":4284,"score_norm":0.861,"seq_a_single":29,"seq_ab_match":68,"seq_b_single":29}
tctttccccacacagcgctcttccgatcnccagagtacgcccattgagtctctgcacctatctcattttcgagataaaatttggctcaggattgcccgcgtactctggagatcggaaggcggttcagcagaaatgcg
+
#############################LLLLLLLLLLEEFDdd^dbT`\`]a`Fa``ddcddbddddddbd_ddddddaadd]dTdd`]b^\_dda`d\bbd]dadC############################
@HELIUM_000100422_612GNAAXX:7:1:8667:17326#0/1 {"ali_dir":"left","ali_length":76,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":76,"pairing_fast_score":0.89,"pairing_mismatches":{"(A:02)->(C:34)":103,"(T:25)->(C:34)":92},"score":3567,"score_norm":0.974,"seq_a_single":32,"seq_ab_match":74,"seq_b_single":32}
ccagcagctcacggcatgtgccaaggaaaacaaaacttaagaagggtgcgtcacgtgttgtcccgatttcggtgtgcacacgggtcgtggcctctcattaaccataaacgactctcggcaacggatatcagctgctgtgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCbcb\`cebbeeaXbc\ba[X`Zdadca^YL`XV\T_aa@ELCP\Z`db[[Z\WNQR?KcCccDEEAEBEELEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8635:4668#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":98,"pairing_fast_score":1,"score":4598,"score_norm":1,"seq_a_single":10,"seq_ab_match":98,"seq_b_single":10}
ccatgcagtgcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccctttctttgttttctcaaaacacggatttggctcaggattgcccgcactgcatgg
+
CCCCCCCCCCEEEEEEEEEEEEEEEc\\a__`\eeeeebeeeee_`]eed`feeefcd_eeeUddedddd_ddeddbe_d_eeeea_Z`aEEEEEEDEEEEEEEEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9354:1871#0/1 {"ali_dir":"right","ali_length":52,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":49,"pairing_fast_overlap":52,"pairing_fast_score":1,"score":3742,"score_norm":1,"seq_a_single":56,"seq_ab_match":52,"seq_b_single":56}
agacacggcgaccgacgagatctacactctttccctacacgacgctcttccgatctcccggtactagaacaggctcctctagcatagtggggtatctaagcaccgtggagatcggaagagcggttcagcaggaatgccgagaccgatctcgtatgcgttcttct
+
########################@-A7@:9>>8;+.:=C:0CCCCC?B>CCCCC@e_e_debeeeeebeeeeebbeeeeedecdeeeddebadeeeeedJedeeeeb>CCC@CAC>CC=BC@BABA?A=A#################################
@HELIUM_000100422_612GNAAXX:7:1:8695:11958#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5708,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccacgagacgtgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggacgtctcgtggag
+
BCacca_`WZY]]c`]cc]ZJYXbeeacaZ_a\ada_adcUddddeedcddddeddeeedeedcd]Za^aaaeeeaeZYYLZYSXXZbbEEEEEEEEEEEEEEEEFEE##
@HELIUM_000100422_612GNAAXX:7:1:8643:4255#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5902,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
ccctacaccacgccttccgatctccttaggatggtcgccccaaccgaatctaggaatatgatatcatatggttagataaagctctatagggtcttcttgatcctaaggagatcgaaagagcggtccagaag
+
#############?5@8=+;BCCaeceebeae\eaaeedceececdeecedffeeeeedddeebddfeedbeeeeeedee_]ebceee_aa_acddccdc^`SRRORO??#####################
@HELIUM_000100422_612GNAAXX:7:1:8683:10622#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":103,"pairing_fast_score":0.96,"pairing_mismatches":{"(C:02)->(G:34)":102},"score":4565,"score_norm":0.99,"seq_a_single":5,"seq_ab_match":102,"seq_b_single":5}
ccagcgtgactgggcaatccgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcgtacacgtgg
+
CCCCCEEEeee_eLcaaaaa[ec_a[_\ca^b``Z_cYeeb\deeedddeeeedeee_edd]]^dc_eebacccEEEEEEEEEEEEEFEEEEEEEEEEEEELEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8792:7907#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acacaac:acacaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acacaac","obimultiplex_forward_tag":"acacaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacaac","obimultiplex_reverse_tag":"acacaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":63,"pairing_fast_score":0.983,"pairing_mismatches":{},"score":2762,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC@LEEd``]d_daabPQPKQebbcaY^^`cZV_]\ba\cOc^]\ZTY[XaEECEBEEEDEDDEEECCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8623:10807#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":86,"pairing_fast_score":0.952,"pairing_mismatches":{"(G:33)->(T:02)":31},"score":4396,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ccacacgcctctcttccgctctaccggcgagcccttcgagggctctgtcggctgccgaggccaacagaaaaaaatatgactctcggcaacggatatcctacgcagtggagatcggaagagcggttcagca
+
######################DEEEEEEEKDEE;EAEBEEEEbMb_b[]dca_ced`dbS`ce]cccccb\[^U[[^dadacaa`aedcbdda`aaaadaa`ad``dABA###################
@HELIUM_000100422_612GNAAXX:7:33:6853:20009#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:gcctccc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctccc","obimultiplex_reverse_tag":"gcctccc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":58,"pairing_fast_score":0.927,"pairing_mismatches":{},"score":3884,"score_norm":0.983,"seq_a_single":50,"seq_ab_match":57,"seq_b_single":50}
tttt
+
eeec
@HELIUM_000100422_612GNAAXX:7:1:9202:6388#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtaatta:gtaatta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtaatta","obimultiplex_forward_tag":"gtaatta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtaatta","obimultiplex_reverse_tag":"gtaatta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":35,"pairing_fast_overlap":62,"pairing_fast_score":0.593,"pairing_mismatches":{"(C:02)->(T:34)":65,"(C:32)->(A:02)":42,"(C:34)->(A:02)":30,"(G:28)->(C:02)":41,"(T:34)->(A:02)":26},"score":333,"score_norm":0.887,"seq_a_single":46,"seq_ab_match":55,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCDCCCCCCCCDCCDCCCCDDLDEELDDELEEEDB=AC?CFJCBBBEBEC:@=PUWT_\_CD=DLBEEEEEEEEEELBEE@CCCCCCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8662:11577#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":101,"pairing_fast_score":0.878,"pairing_mismatches":{"(A:34)->(C:02)":12,"(C:16)->(A:25)":48,"(G:34)->(T:02)":15},"score":3882,"score_norm":0.96,"seq_a_single":7,"seq_ab_match":97,"seq_b_single":7}
cccatctccagactgctcggatgacgccaaggaacacggtaaagctaaggcaccggcgagccaacagaaaaaaatatgactctcggcaacggatatcgagcagtctggagaccgg
+
#######EEELLEELEEEEEEEEEEEEEEEEEEEeWee_`]e]_`]_;dab\c\c_eeaeadcba`bb_bbdba`dMXJT[]ca_`e_U_cZaXaEEDEEEEEEEEEE#######
@HELIUM_000100422_612GNAAXX:7:1:8630:4542#0/1 {"ali_dir":"right","ali_length":83,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":83,"pairing_fast_score":1,"score":3782,"score_norm":1,"seq_a_single":25,"seq_ab_match":83,"seq_b_single":25}
tccctacacgacgctcttccgatcttccactgtcgaccattgagtctctgcacctatcctttattttttgaaagcgggtgttggctcaggattgccctcgacagtgggagatcggaagagcggttcagaagaa
+
########B,><BB?A8B=8?ACC3We[USPX_OZac^acacdbbbbcc_afceccccabcdfcfY`Y[YXe`U]Jcc6KZ`cdDEFEEEEEEECDEEFEEEFE7DEE#########################
@HELIUM_000100422_612GNAAXX:7:1:8642:9015#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":3856,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
ccactgagtgcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctcctggttttctcaaaacacggatttggctcaggattgcccgcactgcagtgg
+
CCCCCCCEEEEEECEEEEEEEEEEEEEEEEbeUeec]c`cb^c^_e[eZb``eebeeeece_ebe\Y[^aeeeabbeeedbccbcEEDEEEEEEEEEEEEEEEEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8680:11414#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5415,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccacgctagctccattgagtctctgcacctatccctttttttttcgttttctgaatctttgttttaagaaaacaggatttggctcaggattgcccagctagcgtgaag
+
##EEEEEEbaKXbb^e_e`edccdca^bbSYcW^`b`[`b_ceddddaee^eebda^edbddebed]cbedda^\be__ed^ca]eeEEEEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8704:1727#0/1 {"ali_dir":"left","ali_length":88,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":88,"pairing_fast_score":0.953,"pairing_mismatches":{"(G:34)->(A:06)":29},"score":4726,"score_norm":0.989,"seq_a_single":20,"seq_ab_match":87,"seq_b_single":20}
ccgatgacgattcggttggggcgaccaaggataactcatccttataaaaaaagataaatatatccaactacagaccctgtacaacagactacaaacaaaagctaccttagggataacagcgtcatcgg
+
CCCCCCCCCCCCCCCCCCCCaU\c\_ccG_^UR^QY\\LU\IRdXdddad^bdad`eecfbe]]ceae]`_c_\_]a\_YRZZVYeaec^SYVVPdddEEEEDEEEEECCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8968:12367#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acaatgg:acaatgg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acaatgg","obimultiplex_forward_tag":"acaatgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaatgg","obimultiplex_reverse_tag":"acaatgg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":52,"pairing_fast_overlap":62,"pairing_fast_score":0.881,"pairing_mismatches":{"(g:02)->(t:34)":81,"(t:26)->(g:07)":35},"score":1517,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCBCCCCCCCCDLEE`XcXSbccZRF?KPTAMW8KIQOMR[\[_]H[BD@AA;:DEECE?EEDEEEEEC?CELCBC?CCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8667:2473#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":73,"pairing_fast_score":0.786,"pairing_mismatches":{"(A:02)->(G:34)":102,"(A:30)->(G:02)":54,"(T:02)->(C:34)":98},"score":699,"score_norm":0.945,"seq_a_single":35,"seq_ab_match":69,"seq_b_single":35}
ccacgtagtcggcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatccgactacgtgg
+
CCCCCCCCCCCCCDCCCCCCCCCCCCCCDCCCCCCBBEEEEBEEEEEDBEBABHE?CBB>A?9?AAaWNVWYPK__Z[aDA=EEBEE=EEEECELC?LEDELEEDEEDCCCCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8639:9977#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":85,"pairing_fast_score":0.744,"pairing_mismatches":{"(A:02)->(C:32)":84,"(A:34)->(G:02)":26,"(A:34)->(T:02)":39,"(C:34)->(T:02)":38,"(G:34)->(A:02)":36,"(G:34)->(T:02)":37,"(T:02)->(G:32)":81,"(T:34)->(A:02)":53},"score":977,"score_norm":0.906,"seq_a_single":23,"seq_ab_match":77,"seq_b_single":23}
cctacacgacgctcttccaatctccactcgctacgggcaatcctgagccaaatccttaagttaaaatcaaaaaaggataggtgcagagactcaatgggtagcgagtggagatcgaaggagaggttcagaag
+
#######################EELEDEEBBB>ELLLLEEEEEEEEEEEEELEEE>E:=A>^cc\^JccccUb^V]bCCJCCJCCCCCCBCCCC@C==7:DC6BDBB#######################
@HELIUM_000100422_612GNAAXX:7:1:8955:2689#0/1_sub[25..123] {"ali_dir":"left","ali_length":66,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agtcgtc:)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtcgtc","obimultiplex_forward_tag":"agtcgtc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_primer":"tagaacaggctcctctag","pairing_fast_count":59,"pairing_fast_overlap":66,"pairing_fast_score":0.937,"pairing_mismatches":{},"score":1103,"score_norm":0.985,"seq_a_single":42,"seq_ab_match":65,"seq_b_single":42}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCBCCCCCCCCCCCCEBELEEEEEEEBEEEEEDDBDDBECECEEEB@@EECC=9=;=@eQ[``__bbad_Ydba?A9ABEECDBCCCADDCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8632:19131#0/1 {"ali_dir":"left","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":84,"pairing_fast_score":0.938,"pairing_mismatches":{"(G:23)->(T:08)":58,"(G:34)->(A:02)":25},"score":3824,"score_norm":0.976,"seq_a_single":24,"seq_ab_match":82,"seq_b_single":24}
cccgacgatctccgaaggcgtcaaggaacactgtgccaaacatgggggccatgcctagcttgctagtcgtgccccatgttgcgaagctatataaaccacacgactctcggcaacggatatcagatcgtcggg
+
CCCCCCCCCCCCCCCCCCCCCCCCLEEEEEEEAc`ZccNcbZ\eUcecaaSOKHSVG;V`_]]`OMSXVVcdccTeb_be][]\RYVUKYYa\UWeZScbECFEEEEECCCCCCCCCCCCCCCCCCCCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8631:8637#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":5435,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccaattaacactaggattagataccctattattttggagcgttaaattttgtgcctgagtagtattagttatgttcttgagttaattggagatcggaagagcggttca
+
A==7==??8848'8===ACbdee^cdcdbecea^^a]]ceebeededdeeeeeddededeeeeeeeeeeedeec^^\aYRRRRQ_____ccaacbceeEEEEEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8661:12022#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":101,"pairing_fast_score":0.735,"pairing_mismatches":{"(A:02)->(G:34)":76,"(C:02)->(T:34)":98,"(C:06)->(A:34)":65,"(C:34)->(T:02)":8,"(G:02)->(C:34)":104,"(G:34)->(T:02)":20,"(T:02)->(A:35)":94,"(T:02)->(G:34)":108},"score":2684,"score_norm":0.911,"seq_a_single":7,"seq_ab_match":92,"seq_b_single":7}
ccgaaccccacgacagcagggcaatcctgagccaaatcctgttttacgagcaattcaaaaaggaatcaaaaataggataggtgcagagactcaatggtgctgtcgtggagatcga
+
#######LEEEEEEEEEEELEEEEEEEEdZbS_[]\TZY^c`eecba`c]]c[^WYaacaYa[aGa^bccccEEELEEEEEEEEEEEEEEEEEMEELLEEEEELEEEL#######
@HELIUM_000100422_612GNAAXX:7:1:8692:3979#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":79,"pairing_fast_overlap":90,"pairing_fast_score":0.908,"pairing_mismatches":{"(A:34)->(T:02)":34,"(T:02)->(A:34)":99},"score":3127,"score_norm":0.978,"seq_a_single":18,"seq_ab_match":88,"seq_b_single":18}
acgacgctcctcccatctccacacgtaggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaatacgtgtggagatcggaggagcgtttc
+
##################EEEEEEEEAEEEEEELDEEEEEEEEeec```ecb_dba]Zecbccdeeeee^eedb^b^[YWZQWRRNQRc]dEEEAEDELEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8613:6779#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(C:02)->(G:34)":104},"score":5455,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagctagtgcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggcactagctgg
+
CCCCCCVae`e_eccUbedeaecdceeeceeeeec_ebeaebceeeeebdeecebeec\daebc`\`bb_`]X\a\cc`ceZceEEEEEEEEEEEEEEEEEEELEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8673:18350#0/1 {"ali_dir":"right","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(A:34)->(C:02)":64,"(A:34)->(T:02)":70},"score":31,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ccgccgagatctacgctctttccctacacgacgctcttccgatctaggggcaatcctgagccaaattctacctaggataggtgcagagactcaatggctgctgtgtggagatcggaagagcggttcagcaggaatgccgagacggatatggta
+
#############################################FEEEEEEEEEEEEEEEEELEEEEELEEEEEDEEEEBDED?CECCBFFDEEEDDBF@EEDEDE=?CC@?@AC@CACACCB@<3;/7??B?###################
@HELIUM_000100422_612GNAAXX:7:1:8685:14678#0/1 {"ali_dir":"left","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":84,"pairing_fast_score":0.84,"pairing_mismatches":{"(A:34)->(C:02)":37,"(A:34)->(G:02)":46,"(G:29)->(A:02)":41},"score":2423,"score_norm":0.952,"seq_a_single":24,"seq_ab_match":80,"seq_b_single":24}
ccactgctgcaggatgacgccaaggaacacgataaaagctgaggcaccggcgagccgctcaagggctatgtcggttgccaaggccaaggcaacagaaaaaatatgactctcggcaacggatatctgcagcgg
+
CCCCCCCCCBCCCCCCCCCCCCCCEEEEEEDCDEEELD@CGLC?CLEEDDEED`MaaXVYMYLcH\bee_]___^aeZY]XMRTARHYNZ]]T]^^B@BC@@EEE?EBCCCCCBC@CCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8666:6982#0/1 {"ali_dir":"right","ali_length":93,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":93,"pairing_fast_score":0.9,"pairing_mismatches":{"(C:02)->(G:32)":108,"(G:34)->(C:02)":23,"(G:34)->(T:02)":32},"score":3647,"score_norm":0.968,"seq_a_single":15,"seq_ab_match":90,"seq_b_single":15}
acgctcttccgatatccagagagtcgccattgagtctctgcacctatcccccttttcactttgttttcggaaaataggatttggctcaggattgccccgactctctggagaccggaagagcgg
+
###############EEEEEEELEEEEEEEELEEEEEEEEEEac__Scc[b`cc`[cX\Y\RZW`[W_b7\_^[QZ\HQZ`Z^CPVQW`QBZ<G^_b^`U```c`CCJ###############
@HELIUM_000100422_612GNAAXX:7:1:8672:12499#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":104,"pairing_fast_score":0.752,"pairing_mismatches":{"(A:34)->(T:02)":18,"(C:02)->(G:34)":103,"(C:34)->(T:02)":28,"(G:23)->(T:02)":48,"(T:02)->(C:34)":101,"(T:02)->(G:34)":88,"(T:34)->(G:02)":15},"score":1027,"score_norm":0.933,"seq_a_single":4,"seq_ab_match":97,"seq_b_single":4}
ccacgctctcaccattgagtctctgcacctatcctttttttattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccctgagagcgtgg
+
CCCCEEEEEEEEEELEELEEEEEEEEELEEEEEEEEEEEE;DDDDBDAEEEEEEB>CA<aeebbabdfedT]]LaEEAEEDEEEEEELEEEEEEEEEEEELELEEDEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8626:4920#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5888,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
tcccacacgacgctcttccgatctccgccgtgcggtcgccccaaccgaatttttattgggttgaccccaatttaaaataaagctctatagggtcttcttggcacggcgagatcggaggagcgtttcagcaga
+
######@>5@A'A==:A=98*08Aceebebbbeacaec`acd^aa__ad\deeeeddXdddcceeeeeeeeebededeebddUeedeeecebcebdebce]_`]XWZZ8A=A####################
@HELIUM_000100422_612GNAAXX:7:1:8659:8465#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":5243,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ctacacgacgctcttccgatctccaaccaagcaagaagaccctatagagcttaatttaacctactaaataagtaaaaattaaattcggttggggcgaccaggaggcggagatcggaagagcggttcagca
+
#########A8AA69(66?CBC_cdd^cccUebeebeebeaedeceeeYeeeeeeeeeeee^cdcceeebee]cdddeeebedccb_\\\]]bdcbEEEEEEEEEEEE######################
@HELIUM_000100422_612GNAAXX:7:1:8660:9048#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":4366,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccaacacactcaagaacataactaatactactcaggcacaaaatttaacgctctaaaataatagggtatctaatcctagtgtgtgttggagatcggaagagcggtgca
+
996417:::<5:888CCCCeeaec`c__eeVcebeeefadeeceeaeecfbeebeeefdeeedeedefecccdaeeeedEEEEEEEEEEEEEEEEEEEEEEDEEEEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8695:5533#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acaatgg:acaatgg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acaatgg","obimultiplex_forward_tag":"acaatgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaatgg","obimultiplex_reverse_tag":"acaatgg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(A:02)->(T:34)":65},"score":1780,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCC?CaQ^]deedceeb[edT]edYa\_aa_aEC?C@<BDCEEBEEE>EELEEEEEEDEEEELDEELCCCCCCCCCCCACBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8663:11783#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":99,"pairing_fast_overlap":106,"pairing_fast_score":0.961,"pairing_mismatches":{"(C:34)->(G:02)":13},"score":4121,"score_norm":0.991,"seq_a_single":2,"seq_ab_match":105,"seq_b_single":2}
cgccactcgagtcgggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatgggactcgagtggag
+
##EEEEEEEEEELEEEEEacVececacecbd^df_b_eeddbbbdeedcdddeebdeeebca]X^aV^\`a^eccWeEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:8627:6831#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":6005,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
cagtcgactgccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccttcttgttttctcaaaacacggatttggctcaggattgccccagtcgatgg
+
CCCC]`]\[]W[__a__abeeebeee^bccc^d\cScbeebeeaebcedbbccbededceeecddeeceeddeeeeeZaeec_]eeeeZeEEEEEEEE?EEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:9022:7090#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttatgtg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatgtg","obimultiplex_forward_tag":"ttatgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(G:34)":56},"score":1868,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCDCCCCCCCCCCCCCCCDeeddbcdd[ddd[a`Taebbc_bc_`cEABDECEEEL<ADEE?EEEEEEEDEEEEEELEEE@CCCCCCBCCCCCC=CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8703:3435#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":73,"pairing_fast_score":1,"score":4253,"score_norm":1,"seq_a_single":35,"seq_ab_match":73,"seq_b_single":35}
ccagagacagcccattgagtctctgcacctatctttaatattagataagaaaaaatattatttcttatctgaaataagaaatattttatatatttctttttctcaaaaagaagatttggctcaggattgccccgactctctgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCedeeeeeeeeddddddedddddddeeeeecdcbcc_\aca_cc_`cc_ccdd]d^^\\W`DDEEEEEEBDDEECCCCCBCCCCCBCCCCCCCCBCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8658:17940#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":101,"pairing_fast_score":0.959,"pairing_mismatches":{"(A:34)->(G:17)":27},"score":5427,"score_norm":0.99,"seq_a_single":7,"seq_ab_match":100,"seq_b_single":7}
ccagcacacagccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccctttttttttctcaaaacacggatttggctcagattgcccctgtgtgctgg
+
CCCCCCC]YYa`\ba_Kd^eaaa\_UDMebdbeedbbbbedec_ea`__`[`dbedeebefeecdeddddedbbdd_eeccceaeY_cEEEEEEFEEEEEEECEEEEECCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8700:2116#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":100,"pairing_fast_score":0.99,"pairing_mismatches":{"(C:34)->(T:02)":9},"score":4832,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tccgatctcccactgtgacgggcaatcctgagccaaatcctgggttacgcgaacaaaccagagtttagaaagcgggataggtgcagagactcaatgggtcacagtgggagatcgga
+
########LEEEEEaX][_e`eeZ]_X_bbc_eec`cdeeedebdeceeceeaecdfeeeeaebec`ddecbedebce__[YaEEEEEEEEEEEEEEEEEEEEEEEEE########
@HELIUM_000100422_612GNAAXX:7:88:1816:12461#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":44,"pairing_fast_overlap":62,"pairing_fast_score":0.746,"pairing_mismatches":{"(A:02)->(G:34)":104,"(C:35)->(A:02)":50,"(T:34)->(A:02)":53,"(T:34)->(G:29)":66},"score":639,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ccgaatatcttagatacccccactatgcttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccctctagaggagcctgttctagatattcgg
+
CCCCCCCCCCCCCCCCCCCCCCBBCCCCCCC@CCCCBCCCCCCCCCEBEMEALCBAEEE__US[_CPc]WVQT7A@::>DAEBD=BC:E=EEEDEEB>EEDEBLE4EBC@CCCCCCCCCCDC@CCC@CCCCCCCCCCCCCCCCBCCC8CCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9130:11082#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gaatatc:ggaggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaatatc","obimultiplex_forward_tag":"gaatatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ggaggaa","obimultiplex_reverse_tag":"ggaggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(A:34)->(T:02)":19},"score":1134,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCBCCCBCLDEcccc^[e`Xec[bVbZ]A=1CCCC?22/2-E?ACECEE?:DEEEDEEEEFEEEEKEEELECCCCCBCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:18:13054:1986#0/1_sub[29..128] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gaagtag:aagtagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gaagtag","obimultiplex_forward_tag":"gaagtag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":2,"obimultiplex_reverse_match":"aggaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aagtagt","obimultiplex_reverse_tag":"aagtagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":61,"pairing_fast_score":0.983,"pairing_mismatches":{"(c:02)->(t:34)":81},"score":3133,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCBCCCCCCCCBCCEEEd`c[VY[aY[]IR^Wa^`\acac_[Z`[_ccebdb`aJPTbcZcW_`WPccEDEBE?LCCCCCCCCCCCCCDCCCAC
@HELIUM_000100422_612GNAAXX:7:114:2790:8855#0/1_sub[28..127] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (attaact:aattaac)","obimultiplex_forward_error":2,"obimultiplex_forward_match":"taggataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"attaact","obimultiplex_forward_tag":"attaact","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":61,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":2600,"score_norm":0.984,"seq_a_single":47,"seq_ab_match":60,"seq_b_single":47}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCEIEEFEDDb^b_XQMPOR[]aVIZ[Y[\SRYY[aVa]^Y_[[S\bcaceaceEEEEEEEECCCCCCDCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8824:1754#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tggtcct:tggtcct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tggtcct","obimultiplex_forward_tag":"tggtcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tggtcct","obimultiplex_reverse_tag":"tggtcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(c:02)->(a:34)":74},"score":2906,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaatagttacacaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCEBAEceXZ[_baaTQVO`JS__SYXXXWRP_VW[b\RaabZ`OVa_cbZbbabaLEEECEEEACCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8819:19971#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (taatcat:taatcat)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taatcat","obimultiplex_forward_tag":"taatcat","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taatcat","obimultiplex_reverse_tag":"taatcat","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(T:22)->(A:07)":35},"score":1356,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCBBCCCCCCCC??]SZTd^bWS[YWd`T;Z_^NW[X`CDACC6?C<D>BCEDDECAEEEDDEDEDEEEEFLBE?ECCCDCCCCCCCCCACCCBC
@HELIUM_000100422_612GNAAXX:7:1:8690:8263#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":84,"pairing_fast_score":0.951,"pairing_mismatches":{"(A:10)->(G:34)":90},"score":5333,"score_norm":0.988,"seq_a_single":24,"seq_ab_match":83,"seq_b_single":24}
ccctacacgacgctcctccgatctccagcacatgcccattgagtctctgcacctatcctttgttttcttaacataaagatttggctcaggattgcccgcatgtgctggagatcggaggagcgctccagcaga
+
#############A:*B<C8CBCAdd`^cb`aZIa[`^^a_cbcccccbebeeeeeeddebbdebddebdcedeeeda]ecbea___`_F\X\[]aVa\\Zc_cEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8670:4246#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4635,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
cccgactcatgccattgagtctctgcacctatccttttttattataactttctgaatccttggttgttttcagaaaacaggatttggctcaggattgccccatgagtcggg
+
CCCEEEEEEEEEEbc_ddadeecb]ecc^cdcbddddeeeedddeedeeeeeeefdec_eee]deeceecRY[[]accEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:9016:19339#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ggaggaa:acagaca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ggaggaa","obimultiplex_forward_tag":"ggaggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acagaca","obimultiplex_reverse_tag":"acagaca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(c:15)->(a:23)":68},"score":1378,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
ACCACCACCCCCCCCCBCLEEEDDEEEDCEEEB@E@@:22101=5>0=EAE@A?C?Z]aU[^Z`^XY9OJLV[ccMd[[[_BCCACBACCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8668:11462#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":105,"pairing_fast_score":0.922,"pairing_mismatches":{"(G:34)->(A:05)":12,"(T:02)->(G:34)":100},"score":5697,"score_norm":0.981,"seq_a_single":3,"seq_ab_match":103,"seq_b_single":3}
ccacgctctcagggcaatcctgagccaaatcacgttttccgaaaacaaacgaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggtgagagcgtgg
+
CCC\STZaY_cH]]`W^[Z^^eddLeaa^Y\daJ_ece_bVdbeededdec`ecc`edcceeedf^\_bbe```_[dd]cRQQJR\[YY^c`^aEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8655:18659#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":106,"pairing_fast_score":0.835,"pairing_mismatches":{"(A:02)->(G:34)":81,"(A:34)->(G:02)":47,"(A:34)->(T:02)":29,"(C:02)->(G:34)":97,"(T:02)->(G:34)":108},"score":1711,"score_norm":0.953,"seq_a_single":2,"seq_ab_match":101,"seq_b_single":2}
ctccacgcactaggggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggctagtgcgtggag
+
##EEEEEEEEDEBBEEEEEBDEEEEEEELBEBEEECE=@AD@BEEELE@abbee__WZaYaa_`aR_ZRJXQd[b`aEEALEBEAEEEEE<EEEAELEEEEEDEEEEL##
@HELIUM_000100422_612GNAAXX:7:1:8757:4288#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tatcagc:tatcagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatcagc","obimultiplex_forward_tag":"tatcagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatcagc","obimultiplex_reverse_tag":"tatcagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":1175,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCC;CEEBEEEEEBccdcZYGZ[SSa`Y\]\\Y<<<<<EEEECEDEDDEEEEEEDEEEEEEEEEEEECCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8695:1848#0/1 {"ali_dir":"left","ali_length":91,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":91,"pairing_fast_score":0.875,"pairing_mismatches":{"(A:02)->(T:34)":106,"(C:02)->(A:34)":95,"(C:34)->(A:02)":24},"score":1993,"score_norm":0.967,"seq_a_single":17,"seq_ab_match":88,"seq_b_single":17}
cctgtaacattcggttggggcgaccaaggaaatatcatcctaaataataaagatcaataaatcaaattacagatcctaacacatagatcatagaacaagctaccttagggataacagtgttacag
+
CCCCCCCCCCCCCCCCCEEEEEELEEEEEEEEEEEEEEEEFdcccVcedeadb_^Seebee_\^eccVccEEDEEECFEDEEEEEEEFEEEEEELEEEEEEAECELEECCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8661:11716#0/1 {"ali_dir":"left","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":5173,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ccacgctctcagggcaatcctgagccaaatcacgttttccgaaaacaaacgaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggtgagagcgtg
+
CCa_eZ\[]Zb^`cdYXUPX\^a\RaaY`]e`eb]eecdeb`beee__cdb_]cc_bcccbeeabaacc`cYY]][ddbdEEEEEEEEEEEEEEEEEEEEEEEEEEEECC
@HELIUM_000100422_612GNAAXX:7:1:8629:2828#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":78,"pairing_fast_overlap":100,"pairing_fast_score":0.804,"pairing_mismatches":{"(A:02)->(G:32)":106,"(C:02)->(A:34)":102,"(C:30)->(G:02)":25,"(T:02)->(G:34)":84,"(T:34)->(G:02)":18},"score":2311,"score_norm":0.95,"seq_a_single":8,"seq_ab_match":95,"seq_b_single":8}
ccacgagcgtcgggcaatcctgagccaaatcctgctttacgaaaacaagggaaagttcagttaagaaagcgacgagaaaaatggataggtgcagagactcaataggacgctcgtgg
+
CCCCCCBCDEEEBEEEELEEECECHCDCD?Cb\YX[^ZYZY`ebe[^RUYee`Ke_c\[U^S^a^`aY`EEDEBBEEEDDCEELEEEECEEEEEEDEEEEELEECJDECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8684:15770#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":102,"pairing_fast_score":0.99,"pairing_mismatches":{"(G:34)->(C:02)":7},"score":5118,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagacgacgaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccagcgtgtctgg
+
CCCCCCLEEFEEEDEEEaP[ca_[X`YT`eeeae_ceeTed]e^eacdceeeedcddebbeeee__aeeedbdeccbeee_eeaTcd\dL_cEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8668:11389#0/1 {"ali_dir":"right","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":98,"pairing_fast_score":1,"score":4969,"score_norm":1,"seq_a_single":10,"seq_ab_match":98,"seq_b_single":10}
cttccgatcgccagagacagcgggcaatcctgagccaaatccggttttccgaaaacaaacaaaagtggaaagggggataggtgcagagactcaatggcgactctctggagatcgtaag
+
?>@5<<*0A+c\aQQIQPa\Wbb_cda_]a^S]RNQQM`M^]Kbccdcbedbdeeddcdbdad[dWc\_]dd_dccecebaccaabcdcEEEEEEEEEEDEFEEEEEE##########
@HELIUM_000100422_612GNAAXX:7:1:8685:15992#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":73,"pairing_fast_score":0.9,"pairing_mismatches":{"(T:02)->(C:34)":98},"score":2503,"score_norm":0.973,"seq_a_single":35,"seq_ab_match":71,"seq_b_single":35}
ccactgagacggcacggcatgtgccaaggaaaacaataaacgagaaggactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatccgtctcagtgg
+
CCCCCCCCCCDCCCCCCCBCCCCCCCCCBCBCCCCDDEcaa]cb[bMW[`VWJ\Qa_ab[aVYW[bc\`c[\_PJ`a_cFEEEEDCEEECEEEELEFLEEEEEEEEEECCCCCCCCCCCCCCCCCCCCDCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8614:5448#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":98,"pairing_fast_score":0.979,"pairing_mismatches":{"(C:02)->(G:34)":107},"score":4714,"score_norm":0.99,"seq_a_single":10,"seq_ab_match":97,"seq_b_single":10}
ccagctagctggggcaatcctgagccaaatccgtgttttgagaaaacaaagaaaggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagctagctgg
+
CCCCCCCCCC``]__]aaaXcbcZad^ceX^eeeeeeeecebddeedcdddedddbeece`ecccebe]beece_^aEEEEEEEEEEEEEEEEEEEEEEEEEEEEELECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8679:7716#0/1 {"ali_dir":"left","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":4488,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
ccagtcagcgtccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccacgctactctgg
+
CCCDCCCEEEEEEFEEEc_]c_cY_]Z\[Zebdeddeebeeeeeeccee^_beebadeeeeeeedbdedeeddeeaeeeeEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCDCCC
@HELIUM_000100422_612GNAAXX:7:1:8635:9628#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":81,"pairing_fast_score":0.923,"pairing_mismatches":{"(C:02)->(T:34)":107,"(T:02)->(A:34)":103,"(T:02)->(G:34)":108},"score":3336,"score_norm":0.951,"seq_a_single":27,"seq_ab_match":77,"seq_b_single":27}
ccagtctctcggatatccgttgccgagagtcatattttttctgttgccttggccttggcaaccgacatagcccttgagcggctcgccggtgcctcagcttttatcgtgttccttggcgtcatcccgagagactgg
+
CCCCCDCCCCCCBCCCCCCC@CCCCCDEBAEEc``O_ed[b`]e`c`addKV]VaX=NZYGRNRQ][U^ZWZ[[U\X\X^K^\^]aLZW[_cEEEAEEEEEELEFLLLCCCCCCCCCCCCCDCCCCCCDCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8660:19141#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":107,"pairing_fast_score":0.894,"pairing_mismatches":{"(C:02)->(A:34)":94,"(C:02)->(G:34)":97,"(T:02)->(G:34)":81},"score":4330,"score_norm":0.972,"seq_a_single":1,"seq_ab_match":104,"seq_b_single":1}
tccacgtactgcgggcaatcctgagccaaatcctgttttccgaaaacaaaggttcagaaagttaaaataaaaaaggataggtgcagagactcaatggcactgcagtgga
+
#EEEc`[c`VLa\FZZUXaQYS\c`ee_]c_]de_eecaeeXZZeeea[``bbeeeac]\cXXXYWbed`_cBCECCEEELEEEEDEEEEEEELDELEEEEEEEEEEE#
@HELIUM_000100422_612GNAAXX:7:1:8659:8591#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":73,"pairing_fast_score":0.757,"pairing_mismatches":{"(A:02)->(C:34)":106,"(G:02)->(T:34)":101,"(T:02)->(C:34)":98,"(T:02)->(G:32)":75},"score":1991,"score_norm":0.932,"seq_a_single":35,"seq_ab_match":68,"seq_b_single":35}
ccacatctgcggcacggcatgtgccaaggaaaacaacatatgagaaggatgcatcctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactcttggcaacggatatctgtcgctgtgg
+
CCCCCCCCCCCCCCCCCCACCCCCCCCCBCCCCCCdaebe``eZc_\```UPPNAIFUBWZSQ[OcbYbcDAC4JEEEBCBEEDEEEEEEEEAELEELAELEEEELEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8633:6069#0/1 {"ali_dir":"right","ali_length":80,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":80,"pairing_fast_score":1,"score":2436,"score_norm":1,"seq_a_single":28,"seq_ab_match":80,"seq_b_single":28}
tctttccctacacgacgctttccgatctccagagtacgcgggcaatcctgagccaagtccatcggatggtcgatggataggtgcagagactcaatgggcgtactctggagatcggaagagcgggtcagcaggaacg
+
0>=:A==A+9<;<?7;;<?:AA=@CCCCd^`[^[a^]RQNQKQS`^\`]a`Z[ddd[_Vd\ccZ^Ua`EDEEEEEEEEEEEEEEFEEEEEDEEEEEEEEEDEEEEEEE############################
@HELIUM_000100422_612GNAAXX:7:1:8754:2921#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gattcta:gattcta)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gattcta","obimultiplex_forward_tag":"gattcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gattcta","obimultiplex_reverse_tag":"gattcta","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:23)->(C:02)":52,"(C:28)->(G:02)":50,"(T:26)->(C:02)":36,"(T:34)->(A:02)":26},"score":350,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgccagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
CCC=CCCBCCCCCCBCCCCDEEEEELDDEEBBEC>D=BBB?BD???;?CF:A<a_^X]WAA4EEEEEECEEFEEEELBEEEBCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8663:4308#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(C:02)->(A:34)":102},"score":5136,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
ccagacgcatcgggcaatcctgagccaaatccttctttccgaaaacaaataaataaaagttcagaaagttaaaatcaaaaaaggataggtgcagagactcaatgggatgcgtctgg
+
CCCDCCCC^be^Xbdd^^U^Y^eaXaeeeecaaa_cebcddddeedddecedddaedbdab__c`ccd_`bddeaXX[[Zd]cEEEEEEEEEEEEEEEEEELEEEEEECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8643:15162#0/1 {"ali_dir":"right","ali_length":108,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":108,"pairing_fast_score":0.771,"pairing_mismatches":{"(A:02)->(C:34)":68,"(A:34)->(G:02)":16,"(C:02)->(T:34)":92,"(C:34)->(A:02)":12,"(G:29)->(T:02)":40,"(T:33)->(G:02)":8},"score":632,"score_norm":0.944,"seq_a_single":0,"seq_ab_match":102,"seq_b_single":0}
atatccgttgccgagagtccgctcgccggcgcatcagaggacttctcgttttatgcccaggcagtgccaggactctacgtgttccttggcgtcatccgcacatcgtgg
+
EEEEEEEKDEELECELECDEEABEEEBC=?<AA=A8C@CGEED:ece\b`a`c99;;6EE=CEEEECLAEEDEECE=EEEEEDEEEDEEEELEEEEEEEEEEEEEEEE
@HELIUM_000100422_612GNAAXX:7:1:8617:2730#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":5251,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctcctaagtctggtagtactctggcgaatagttttgtttatgcaactatttgtgtttagggctaagcatagtggggtatctaaagacttaggagatcggaagagcggttc
+
#####?6A=288A5:<<:]_Zc_cbbcec]eec_eeedafeaeeecdddeeeeddbdbbcbbeeeeeeeeeed]cdcc[`a`[_ca__dcdcEEEEEEEEEEFEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8668:16084#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":89,"pairing_fast_score":0.814,"pairing_mismatches":{"(A:02)->(C:34)":105,"(C:33)->(A:17)":44,"(G:34)->(T:02)":29},"score":2796,"score_norm":0.955,"seq_a_single":19,"seq_ab_match":85,"seq_b_single":19}
ccgaaggccattcggttggggcgaccatggaattcatcatccaccacaaataagattacctaatcactcaaaagatcctaacaatagatcaaagaccaagctaccttagggataacaggttagaagg
+
CCCCCCCCCCCCCCCCCCCEEEEEFEEELEEEEEEEEDEFEEaC`]`cceecccbWc^bd]_^W_\_``^eeeec^_a___c_]cEEEEEEEEEEELEEEEEEELEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9037:3963#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgacac:gcgacac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgacac","obimultiplex_forward_tag":"gcgacac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgacac","obimultiplex_reverse_tag":"gcgacac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":63,"pairing_fast_score":0.9,"pairing_mismatches":{},"score":1577,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaacattcaataaacaagaatgttcgccagagtactactagcaacagcctgaaactcaaaggacttggcggtgctttacatccct
+
CCCCCCCDCCCDCCDCACEEEFEEEEEcefeN__^`_]`]UcaecaSY`bdEBEEE:BCECEEDEEEBE@EEEEEL3EELCCC?CCCCCBC@CCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8686:18537#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aatgtcc:aatgtcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aatgtcc","obimultiplex_forward_tag":"aatgtcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aatgtcc","obimultiplex_reverse_tag":"aatgtcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(t:33)->(a:02)":35},"score":1636,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCBCCCCCCCCCC?LEEEEEEEDDDDDDKEEEE7EE???AC=BB.CD`YH[]`]UZZ]R]ZWW_WZ_\]aLa^Y[BBCCCCCCCCCCC@CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8641:4420#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":73,"pairing_fast_score":0.829,"pairing_mismatches":{"(C:02)->(A:30)":95,"(C:02)->(T:34)":99,"(G:34)->(C:07)":49},"score":2564,"score_norm":0.959,"seq_a_single":35,"seq_ab_match":70,"seq_b_single":35}
ccatgcgagacgatatccgttgccgagagtcgtttatggttattgagaggccacgacctatgcacaccccgcgaacgaggcatcacgagacacgaccttcctatgttttgttttccttggcacatgccgtgcgtctcgcatgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCC=C@CC@E@Eaa`a]U^XccGL]Z`b\[ZbW^_Rdae_e^c\c\`d^bcX`EE?BEE??EECEEECHDDALEDEEDDDDDCCCCCCCCCCCCCCCCCCCCCDCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8800:10443#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agtgcaa:agtgcaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agtgcaa","obimultiplex_forward_tag":"agtgcaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agtgcaa","obimultiplex_reverse_tag":"agtgcaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{},"score":1481,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacataaatcagtctataacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggtggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCSba\dbebXYUU]a]QNWPZc[]cCEEEDB:B<CECCEEEEBDEDEEEEEEEEELEBLDEEEECCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9378:7041#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtacag:tgtacag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtacag","obimultiplex_forward_tag":"tgtacag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtacag","obimultiplex_reverse_tag":"tgtacag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(g:02)->(t:34)":81},"score":3298,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
CCCCCCCCCCCCCCCDCCCELEeeeVXdeeceQLIQRZa[bTQQQQOOMURSW_^]_dcba^bdcbee]a[_cb[aU_ECLCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9283:20678#0/1_sub[27..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atgatca:atgatca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgatca","obimultiplex_forward_tag":"atgatca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atgatca","obimultiplex_reverse_tag":"atgatca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":63,"pairing_fast_score":0.883,"pairing_mismatches":{"(c:02)->(a:34)":74},"score":2688,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCLELDa`acVW]`^[Z\LYcWY_cO[UWZNLILN[[_]\```bW^Z^V^W\_EAEELEEEDEDECCCCCCBDCCCCC?CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8660:8637#0/1 {"ali_dir":"left","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":65,"pairing_fast_overlap":74,"pairing_fast_score":0.915,"pairing_mismatches":{"(A:02)->(T:34)":105,"(T:02)->(A:34)":103},"score":441,"score_norm":0.973,"seq_a_single":34,"seq_ab_match":72,"seq_b_single":34}
ccacatagtcggcacggcatgtgccaaggaaaacaaaaatgagaagggctcgaaccgtgttgccctgtttgcagtgtgcatgcggttcgtggactctttgtaattacaaacgactctcggcaacggatatccgactacgtgg
+
CCCCCCCCCCCCCCCCCCBCCCCCCCCCCCCCCCEDDDDDDEEEEEDCEDECEEE:>CDCC>CDDBEDCDED^Yebb`CDEDEEECEEDE>ECEEEEEEEEELELEEECCCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:5690#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(G:34)":100},"score":5448,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
cccatcgcgaggggcaatcctgagccaaatcctgttttctgaaaacaaccaaggattcagaaagtgataataaaaaaggataggtgcagagactcaatggcagagtcgtgg
+
CCCT]cc^cbdTc_ceeccbe_eeeeeeeeeeeeeaeeeddbeedeeddbcebceeeaaeeeee___^_^dbdbcaZdacEEEEEEEEEEEEEEEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8649:16980#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":105,"pairing_fast_score":0.961,"pairing_mismatches":{"(T:02)->(G:34)":100},"score":5512,"score_norm":0.99,"seq_a_single":3,"seq_ab_match":104,"seq_b_single":3}
ccacgcgtcaggggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggagctagcgtgg
+
CCCEEEEEEEEd`_cd\]Z[[YdZTdc__aade`eeeedddddeedceddeebdcde`]eeeeefaadddeddddd_dTdRQQIR[YY[]ccZbEEEEELEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8658:2266#0/1 {"ali_dir":"right","ali_length":71,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":71,"pairing_fast_score":1,"score":2779,"score_norm":1,"seq_a_single":37,"seq_ab_match":71,"seq_b_single":37}
atctacactctttccctacacgacgctcttccgatcttggcgaataattttgttatattaattacttgtgtttagggctaagcatagtggggtatctaaacgtcacggagatcgaaagagcggttcagcagaattgcggagacag
+
#######################?8?=?;05?7>==<ccUb`aee^addcece``eedeee^a^eeeebeee_c_cEEEEEEEEEEEEEEEEDEEEEEEEEEEEEEEE#####################################
@HELIUM_000100422_612GNAAXX:7:1:8639:9801#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":77,"pairing_fast_overlap":84,"pairing_fast_score":0.951,"pairing_mismatches":{"(G:34)->(T:05)":34},"score":4597,"score_norm":0.988,"seq_a_single":24,"seq_ab_match":83,"seq_b_single":24}
ccctacacgacgctcttccgatctccgcgatgaggtcgccccaaccgaatctttgatcggtttttcgattgtttgataaagctctatagggtcttcttgcctcagaggagatcggaggagcggttcagcaga
+
#####AAA1+;788?/:3A+>CACeebc^d\`cHcdcaed_eeaec`eceece_ebdefeeeeeeecdeeeeeff]eeebe]ebeeecaEEEEEEEEEEEEEEEEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8640:7444#0/1 {"ali_dir":"left","ali_length":76,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":76,"pairing_fast_score":0.836,"pairing_mismatches":{"(A:02)->(G:34)":86,"(C:02)->(T:34)":96,"(T:02)->(A:34)":100},"score":2383,"score_norm":0.961,"seq_a_single":32,"seq_ab_match":73,"seq_b_single":32}
acagacgtgatatccgttgccgagagtcgtttgtgatttcaaggaggccgcatcctgtatgcacaccgcgaacgggacaaagcaggatgcatccttctaatatgttgttttccttggcacatgccgtgcacgtctgtggg
+
CCCCCCCDCCCCCACCCCCCCCCCCCBCCBC@a_[`Reac\ac`b]_MXaVQSMBaa\b`cebedcWcdaCECEEEBEBCEDBDELECEEBEFEELEDELEEEDDEEEDCCCCCCCCCCCCCCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:103:9371:15743#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":45,"pairing_fast_overlap":61,"pairing_fast_score":0.776,"pairing_mismatches":{"(A:02)->(G:32)":104,"(C:02)->(A:27)":96,"(T:19)->(G:15)":66,"(T:31)->(A:20)":48},"score":1775,"score_norm":0.934,"seq_a_single":47,"seq_ab_match":57,"seq_b_single":47}
ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagccctgttctaaggaggcgg
+
CCCCCCCCCCCCCCCCCCACCCCACCCC6C<BBBBCBCCCCCAC=CCA]__`a_YaU_LaXOWZR5U_]^UWXRQSRSNRZ\7>,CEA=BEEEEEECBEECCCJ?=CACCCCC>CCCBCC@?CCCCCCCCCCCCCCCCCCCCCCDCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8740:20474#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtgagcc:gtgagcc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtgagcc","obimultiplex_forward_tag":"gtgagcc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgagcc","obimultiplex_reverse_tag":"gtgagcc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(c:02)->(a:34)":67,"(c:02)->(t:34)":81},"score":2097,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaaataattaaataaacaaaattattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
CCCBCCCCCCCCCCCCCCBELEEefXZWdbe\`WH[aaA_[OVddcb`X__WcR`]]^EEEE?CE*LELEEECELEEEEBLCCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:4839#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:02)->(G:34)":96,"(C:34)->(A:02)":12},"score":4356,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ctatgcagtgcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgcactgcatgg
+
CCCCCCEEEEELEEEEEEEEEEEEEccPcc_UU_eabcdeeeee`c^eec_`eeeceeeefbeePdcefeeddd_\_`_c``\bU^^ZaEEEEEELEEEEEEEEEEEECCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8684:8580#0/1 {"ali_dir":"right","ali_length":101,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":101,"pairing_fast_score":1,"score":5345,"score_norm":1,"seq_a_single":7,"seq_ab_match":101,"seq_b_single":7}
ccgatctcccactgtcgaccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtaacccaggatttggctcaggattgccctcgacagtggggagatcgc
+
####CACeR]TVX^W\\^ZY]MX___c]ecZ`e`ecee\aaaUdeeedeeeddfeeeeeeeeeebdeaadcdeeeeacdcccbbbcEEEEEEEEEEEEEEEEEEEEEE#######
@HELIUM_000100422_612GNAAXX:7:1:9194:3213#0/1 {"ali_dir":"right","ali_length":57,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":54,"pairing_fast_overlap":57,"pairing_fast_score":1,"score":4207,"score_norm":1,"seq_a_single":51,"seq_ab_match":57,"seq_b_single":51}
cggcgaccaccgagatctacactctttccctacacgacgctcttccgatctccttcgccatagaacaggctcctctagaaaagcatagtgggtatctaaaggaggcggagatcggaagagcggttcagcaggaatgccgagaccgatctcgtatgccgt
+
###A=?.B2@9=>A;A?CACACCAA@A9CCA>CCDBCCCCCC?CCCBBCCCdeeeeeeedfeeebeeeeeeeeeeeeeeeeeeedeeeeeedeeeeeeeeeeeeebeeCCB=@ACCCCCC@CCAC?#################################
@HELIUM_000100422_612GNAAXX:7:1:8616:10407#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(G:34)->(T:07)":34,"(T:02)->(G:34)":101},"score":4628,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
ccgcgcacgtagggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaaagataggtgcagagactcaatggtacgtgcgtgg
+
CCAC]X_e]eba[[SUY_XM`cR_Z_]RRLQNUGPdceW_^bbee``a``cd_QYe]aa`d_dacadbddYcaaa[bb_bEEEEEEEEEEEEEEEEEEEELEEDEEEFCCCC
@HELIUM_000100422_612GNAAXX:7:1:8654:8828#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":5566,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccttcaacgttagataccccactatgcctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactacccgctgaaggagatcggacgagcgtttc
+
4=8A9C?ACCC;CCA@<@adcddcca\ad__^bZa__`\cddabddddYcdd`dcdabdddddd`ddccX[^[dccb_\[db`_`]`\d`b^TU\\\\bbEDEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8637:9429#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":73,"pairing_fast_score":0.757,"pairing_mismatches":{"(A:02)->(C:33)":95,"(A:34)->(G:19)":45,"(C:02)->(A:34)":104,"(G:26)->(T:08)":44,"(T:31)->(G:09)":69},"score":2175,"score_norm":0.932,"seq_a_single":35,"seq_ab_match":68,"seq_b_single":35}
ccatgcagtgcgcacggcatgtgccaaggaaaacaaaacataggaaggtcgcgtctcgtgatgcctcgttcgcggggtgtgcatgggtcgtggcctctcaataaccataaacgactctcggcaacggatatcgcactgcatgg
+
CCCCCCCCCCCCCCCCCC?CCCCCCCCC=CBCCCC_baU\WY]>DMJSOPaa^X`IWU`bbR][ROS^C\XW\[ZcR\eEEBAEEEEEDEDDEEKEEDEEEE=LEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8625:18507#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":103,"pairing_fast_overlap":106,"pairing_fast_score":1,"score":6904,"score_norm":1,"seq_a_single":2,"seq_ab_match":106,"seq_b_single":2}
ctccagacgtacggggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggcgacagtgtggag
+
><W]X`ZZ_bSbbce]_deededeeeeafddeec]cdcee^d_`dee`^dbdceddYaccd_[^^ZZcc]ccebdaecc_`cc]]_ceb_bZ[\\[VYYVSXQRQQQ[AA
@HELIUM_000100422_612GNAAXX:7:1:8659:9185#0/1 {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":63,"pairing_fast_score":0.867,"pairing_mismatches":{"(T:02)->(A:34)":94},"score":1301,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
gatatcccccaacataccaaaactagctgtcactccaccagttgtcggagatgtaaggattgatacataaaataactttttatttaattgataatcatataaagcagacgatattttagccatttgcatcaagctcaaacttccttcctgcat
+
CCCCCCCCDCCCC@CCCCBCCCCCCCCCCBCACCCBCCB@?@CC@CEBEEACB?E@E3AA[XYWZT[[\Ta_ebPOP^_^acBCEALEE@AEDLEEEEEEECEEEEEECCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8695:3301#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":103,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(G:34)":86,"(C:02)->(A:34)":99,"(T:02)->(G:34)":102},"score":4276,"score_norm":0.961,"seq_a_single":5,"seq_ab_match":99,"seq_b_single":5}
ccatctgcgtcgggcaatcctgagccaaatccgtgttttgagaaaacaaggggttctcgagctagaatccaaaggaaaaggataggtgcagagactcaatgggacgcagatgg
+
CCCCC\a\b_cIdeceeebd^dd[beeebeeeeeeeeeeeeddeeeedbdeeceeeebadcZ^ccaEEEEEEEEEEEEEELEEEELEEEEEEEEEEEELEELEEFEEECCDCC
@HELIUM_000100422_612GNAAXX:7:1:8689:17023#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":106,"pairing_fast_score":0.786,"pairing_mismatches":{"(A:02)->(G:30)":90,"(A:34)->(G:02)":5,"(C:02)->(T:34)":104,"(C:34)->(T:02)":35,"(T:34)->(A:02)":38},"score":1865,"score_norm":0.943,"seq_a_single":2,"seq_ab_match":100,"seq_b_single":2}
ctccacgactctgccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgccccgtacgtctggag
+
##EELEDEEEEA@EEAEEEEEEEEEEEEEEEEEBLEDLEEED@8@-C=\aaIbbIZcb`]`[`de_`X`cee`b__aEEEEEEEEEEEEHEEEEEEEAELEBELAEEE##
@HELIUM_000100422_612GNAAXX:7:1:8667:9208#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":89,"pairing_fast_score":0.814,"pairing_mismatches":{"(C:02)->(T:34)":97,"(G:34)->(T:25)":50,"(T:02)->(G:34)":103},"score":3203,"score_norm":0.955,"seq_a_single":19,"seq_ab_match":85,"seq_b_single":19}
ccttcgccactgttatccctaaggtagcttggtctttgatctattgttaggatcttttgagtgattagataatcttatttgtggtggatgatgaattccatggtcgccccaaccgaattggcgaagg
+
CCCCCCCCCCCCCCCCCCCEEEEEEEEEEccbe]c^de`ae_cefeabaCec_ceeeedceeee]XZYU\WKY][``YcEEEALEEEEEEEEEEEELEEEEELEEEEECCCCCCCCCCCCCCCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8631:10388#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":63,"pairing_fast_overlap":81,"pairing_fast_score":0.808,"pairing_mismatches":{"(A:02)->(C:34)":92,"(A:02)->(T:34)":95,"(G:33)->(T:02)":39,"(T:02)->(C:34)":100},"score":1711,"score_norm":0.951,"seq_a_single":27,"seq_ab_match":77,"seq_b_single":27}
cccagtgtgtgccgaaggcgtcaaggaacactgatattgccatgcgagggtgagggctagcttgctggcctaaccccaagcagcgatcaaccattaatccacacgactctcggcaacggatatccacacactggg
+
CCCCCCCCCCCCCCCCCCBCCCCCCDCEEEEEDEDEEEKEEEDBEEEEddZYcc_K]YDNSVQUXKK?XQaRb_ba[_MTCCDD:ECECEELC@LCEEELEEEEEEEECCCCCCCCCCCCCCCCCCCCDCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9255:17479#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttatgtg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatgtg","obimultiplex_forward_tag":"ttatgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(a:02)->(g:34)":77},"score":2903,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCDcE[db_ddVbb`bKPLQQT`Rd\a^a__WVUUY\_\Zb_cd]]cac\^dDEEEEEFELDEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:92:12769:10854#0/1 {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":53,"pairing_fast_overlap":61,"pairing_fast_score":0.914,"pairing_mismatches":{"(A:02)->(G:34)":104,"(A:02)->(T:34)":108},"score":1974,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ccgcctcctttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttatacccttctagaggagccttgttctaaggaggcgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC@CCUcebdc]]a__Pe\dZccP\X_^]^^^[^V_B=EEECDBEBEECEEEEDEAEEEEELEEELCCCC@DCCCCCADCCCCCCCCCCCCCCCCCCCACCCBCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8704:1698#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":90,"pairing_fast_score":1,"score":6157,"score_norm":1,"seq_a_single":18,"seq_ab_match":90,"seq_b_single":18}
acgacgctcttccgatctccttcctggttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccccaggaaggagatcggaagagcggttc
+
################C=_d_a[d`_b`a^bedTbbee[cPedae`deYe\ebbeeeeee`faeeeefeae_]eeeeeddabZa`_caeccc^]_Z_caeaecP_Wa\@A0:9?1=8>89?#####
@HELIUM_000100422_612GNAAXX:7:1:8656:1526#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":4799,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacacgacgctcttccgatctccggaggaacaagaagaccctatagagctttatcaaacaatcgaaaaaccgatcaaagattcggttggggcgaccttcctccggagatcgcaagagcggttcagcaga
+
#@=0CBCC8=C?@C?8CB?=3??:cd\dece_eeedebecbadeaeeeeeceeeeeeefeeeeeefd_eeeeeee`ecccee`c^^^[^WaEEEEEEEEEEEEEEFEE########################
@HELIUM_000100422_612GNAAXX:7:1:8622:20661#0/1 {"ali_dir":"left","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":94,"pairing_fast_score":0.923,"pairing_mismatches":{"(A:02)->(G:34)":106,"(G:33)->(T:02)":27},"score":1679,"score_norm":0.979,"seq_a_single":14,"seq_ab_match":92,"seq_b_single":14}
tgccaaggaaaacaacatattagaaggatgcatcctgctttgtcccgttcgcggtgtgcatacaggatgcggcctccttgaaatcacaaacgactctcggcaacggatatcacgtctgtggg
+
CCCCCCCCDCCBCCBBEEEEEEEEEEKE?=?ACa]c^WVeaea`]`c_]We\Z^ac^`DDEBEEEEDEDEEEEEEDE=DBEEECFEEEEEEDEEEEEEEEEEEEELEECCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8673:2881#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4040,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacgcgtcaggggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatggctgacgcgtgg
+
CCCEEEEEEEEEEEEEEEEEEaecLacc`Zc^ea__ce_eO]beebb\dbde_db_^b]efee]e`abdbe`\`_d_d[eEEEEEEEEEEEEEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8669:8006#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agatcta:tatctca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agatcta","obimultiplex_forward_tag":"agatcta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatctca","obimultiplex_reverse_tag":"tatctca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{},"score":1533,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCC@CCCCCCCCCCCDCBC`T`]eacV\^^^c`JCQQRLEVQY_b`DD>BDBDCBEAFDEEED=EEFBEEEEELEELCEDLCCCCCCBCBCCCC?CCCCC
@HELIUM_000100422_612GNAAXX:7:112:10203:5391#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:gccacct)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gccacct","obimultiplex_reverse_tag":"gccacct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":58,"pairing_fast_score":0.927,"pairing_mismatches":{},"score":3878,"score_norm":0.983,"seq_a_single":50,"seq_ab_match":57,"seq_b_single":50}
tttt
+
edec
@HELIUM_000100422_612GNAAXX:7:1:8997:3172#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtcagc:tatcagc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtcagc","obimultiplex_forward_tag":"tgtcagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatcagc","obimultiplex_reverse_tag":"tatcagc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1829,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCDDCCCCCCCCACe_eaeeeb_eebeeZQXa[SWXZV`aaaEDECDBEEEEEDEEEEEEEEEDECEEEEELDEEICCCCCC=CCCCCCACCCC@
@HELIUM_000100422_612GNAAXX:7:1:8676:19628#0/1 {"ali_dir":"right","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":86,"pairing_fast_overlap":89,"pairing_fast_score":1,"score":4927,"score_norm":1,"seq_a_single":19,"seq_ab_match":89,"seq_b_single":19}
cacgacgctcttccgatctccgaaggccactaggattagataccctattattttggaatgtaaattgttttgcctgagtagtattagttatgttcttgaggccttcggagatcggaagagcggtgca
+
??>,3==A=33A8?+:>?0MRPO[[]U\][YZ^M\]^]beeaeccb`_beeeec`edfdecebcddedeedde\WYUZPRRRRQXY\]\a^]]ad^a`aEEEEEDEEE###################
@HELIUM_000100422_612GNAAXX:7:1:8625:10678#0/1 {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(A:02)->(T:34)":99,"(C:02)->(A:34)":94,"(T:02)->(C:34)":97},"score":551,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
atgcaggaaggaagtttgagcttgatgcaaatggctaaaatatcgtctgctttatatgattatcaattaaataaaaagttattttatgtatcaatccttacatctccgacaactggtggagtgacagctagttctggtatgttgggggatatc
+
DCCCCCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCBCCCBCCC@@BEBBCAECEEEEA@EADATa[RPVLU^]EDDE@@EDDDBCEBAEEBEDLCDLDLCEEEEEEEECCCCCCCCCCCCCCCDCCCCCCCCC5CCCCCCCCCCCCCCCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8613:19278#0/1 {"ali_dir":"right","ali_length":12,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":6,"pairing_fast_overlap":12,"pairing_fast_score":0.667,"pairing_mismatches":{"(C:08)->(G:28)":107,"(C:31)->(G:23)":97},"score":373,"score_norm":0.833,"seq_a_single":96,"seq_ab_match":10,"seq_b_single":96}
cgccagtcgagcgatgctgagccgaatcacctgttgagttcacaaaacaaagaaaagcccaggaggcgaaaaaaggataggtgcagagactcaatgccactgcagtggggcaatcctgagccaaatcctattttccaaaaacaaacaaagccacagaaggcgaaaatcgaataggagaggagactcaacacaactgtagtggag
+
#####################################################################@@@@@<@@@=@@@@@@@@@@@@@@888@ISX[GD\TW@K0//AAA@AAAAAAAAA@A>0)<>@A@@B>A>AAA##############################################################
@HELIUM_000100422_612GNAAXX:7:1:9010:7403#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gtaatta:tatgttg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtaatta","obimultiplex_forward_tag":"gtaatta","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatgttg","obimultiplex_reverse_tag":"tatgttg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":1764,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttatatccct
+
CDCBCCCCCCCCCBCCBBA]_b`^_de_ddd`dYUYZV]a_\`a_^DECDBEEEEDEEEEDEECDEEDEEBEEEEELEEELCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8615:18591#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":100,"pairing_fast_score":0.835,"pairing_mismatches":{"(A:02)->(G:34)":98,"(C:02)->(G:34)":102,"(C:34)->(T:02)":39},"score":3331,"score_norm":0.96,"seq_a_single":8,"seq_ab_match":96,"seq_b_single":8}
ccatgcgtgcaccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccccccttttttttctcaaaacacggatttggctcaggattgccctgcacgcatgg
+
CCCCCCCCEEEEEEEBEEEEEEEEEEEEEEEDEEEEEEL[aa__bbJc_]_Zcab[bcRec_c^bddb_dbe[X__bcbebcaacK`c\bELEEEEELEEELEEEEFECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8621:14716#0/1 {"ali_dir":"right","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":107,"pairing_fast_score":0.635,"pairing_mismatches":{"(A:34)->(T:02)":32,"(A:34)->(T:06)":56,"(C:02)->(T:34)":104,"(C:30)->(T:10)":52,"(C:34)->(G:07)":36,"(C:34)->(T:19)":42,"(G:26)->(C:19)":64,"(G:33)->(T:21)":60,"(G:34)->(T:02)":20,"(T:34)->(C:02)":33},"score":3215,"score_norm":0.897,"seq_a_single":1,"seq_ab_match":96,"seq_b_single":1}
tccagagtagcgccattgagtctctgcacctatccctttttctttcggaatctttatttgtttgccaaaaaaaaaaggatttggctcaggattgccccgctactctgga
+
#EEEEEEEEEEEEEFEEEELEEEEEEEEEEELLEeGaa]ccD^QQQRRXGKBX[QGY`MC\\@<`Q`bbbccd`d[Wa^bcd]bdcebdcLR[W\\`e^eEEELEEEE#
@HELIUM_000100422_612GNAAXX:7:1:8674:8091#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":92,"pairing_fast_overlap":105,"pairing_fast_score":0.902,"pairing_mismatches":{"(A:34)->(G:02)":11,"(C:02)->(T:34)":107,"(C:34)->(T:02)":15},"score":2876,"score_norm":0.971,"seq_a_single":3,"seq_ab_match":102,"seq_b_single":3}
ccagacgacgagggcaatcctgagccaaccataacagcgacccttggttcctgtcgctcaagaagctttgcgagcggtgataggtgcagagactcaatggtcgtcgtctgg
+
CCCEEEEEEELEDELEEEBEEEEEEEEEEEEEEEaW[W`TW[b_abaada`cc\c\_WaWY]]WcZc^Y_U_Q^ZecbbEEEEEEEEEEEEEEDEEEDEEEDCD?CLECCC
@HELIUM_000100422_612GNAAXX:7:1:8631:9371#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":5959,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccacgactagcgggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaatcaaaaaggataggtgcagagactcaatgggcagtgactgg
+
CCC_c\___acdcTac]UTZYaee]ec_a_aadaceeecdbbdeedddddeeddebe^beeeeeacceec_]_ccebe_bRQQMRa]Y]^ebEEEEEEEEEEEEEEEECCC
@HELIUM_000100422_612GNAAXX:7:1:8617:17515#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":102,"pairing_fast_overlap":105,"pairing_fast_score":1,"score":4061,"score_norm":1,"seq_a_single":3,"seq_ab_match":105,"seq_b_single":3}
ccactagctcggggcaatcctgagccaaatcacgttttccgaaaacaaacaaaggttcagaaagcgaaaagaaaaaaggataggtgcagagactcaatggtacgtgcgtgg
+
CCDEEEEEEEFEEEEEEEFEEEaSM^_a_`cbZXc`_e^ebddeeddacddeddcae_`eeeece`ddd`eeddddddadEEEEFEEEEEEEEEEEEEBEEEEEDEEEBCC
@HELIUM_000100422_612GNAAXX:7:1:8654:5582#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5976,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
ccctacacgacgctttccgatctccggtctggcaagaagaccctatagagctttatctaaccatatgatatcatattcctagattcggttggggcgaccccagaccggagatcggaagagcggttcagcag
+
###########AA=CAAA9;@CCeedddebbaeeebecebbedfeeeee]eeeeeeeeeeeeedeefeeedb]cccccecbcc\^\Y^dccbdeebddeccaeYZIY[BB@####################
@HELIUM_000100422_612GNAAXX:7:1:8663:9577#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":96},"score":4101,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagcgcgtacccattgagtctctgcacctatccttttcctttgtattctagttcgagaaccttcttgttttctcaaaacacggatttggctcaggattgcccgtacgcgctgg
+
CC;CCBEEEEDEEED]C^^ceed`N[VN[^`S\c\`_bQdSZbRcbbb]ZTWaP[`T]PW_be\dbMbeZaJXYY]M`\Y`\aYKaEEEEEEEEELEEEEEEEEECEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8698:16302#0/1 {"ali_dir":"left","ali_length":87,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":87,"pairing_fast_score":0.905,"pairing_mismatches":{"(G:32)->(C:09)":51,"(T:02)->(G:34)":105},"score":3582,"score_norm":0.977,"seq_a_single":21,"seq_ab_match":85,"seq_b_single":21}
ccgcctcctctgttatccctaaggtagcttgtttttttgatctgggtcagggtcttttttgagatgtatatttctttttgtgggtgatgatttgtagagcatcggtcgccccaaccgaataggaggcgg
+
CCCCCCCCCCCCCCCCCCCCC]XX]aaN[```eedbd`]dQ`ddL[RVWcDZW_b_^_US_Z]PZeceb]``^``cWcMcEEEEBBEEB?DEEEEEEEEEDEEELDEECC@CCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:19579#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":104,"pairing_fast_score":0.891,"pairing_mismatches":{"(A:02)->(G:34)":90,"(A:12)->(G:34)":78,"(C:02)->(T:34)":106},"score":3351,"score_norm":0.971,"seq_a_single":4,"seq_ab_match":101,"seq_b_single":4}
atctacgcatgcgccattgagtctctgcacctatccttttttcaccttctgggcctttgtttgtttttggaaaataggatttggctcaggattgccccgcatgcgtggtgat
+
####EEEEDEADAEEDEEEBEbeX]^U]LP]]^`UdZ`_e_bQ_`dKWed_Z``Yab[_]_TZ`VQUNWHeedb^ZaEbBEEEE@EEEELEEEEEEEE=EDEEDELEE####
@HELIUM_000100422_612GNAAXX:7:1:8696:5242#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":80,"pairing_fast_overlap":86,"pairing_fast_score":0.964,"pairing_mismatches":{"(T:02)->(C:34)":106},"score":4004,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
cacgctcatggatatccgttgccgagagtcgtgtggattaaatagcattgcagcacggggtgcggccagcaagccagccgcgtccccgagctaggcacagtgttccttgacgccttcggcatgagcgtgg
+
C@CCCCCCCCCCCDCCCCCCCCeeeeZeeee`ecdebbdee_ecfTSXXWTJTXNQQXWEVQ`caaZY\MRRRRORRRORda_bcdaU[bDEDEEDEECEEEEEFLEDCCCCCCCCCCCCCCCCCCCDCC
@HELIUM_000100422_612GNAAXX:7:1:8642:14255#0/1_sub[28..120] {"ali_dir":"left","ali_length":69,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acacaac:acacaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acacaac","obimultiplex_forward_tag":"acacaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acacaac","obimultiplex_reverse_tag":"acacaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":69,"pairing_fast_score":0.848,"pairing_mismatches":{"(C:02)->(A:34)":59},"score":1569,"score_norm":0.957,"seq_a_single":39,"seq_ab_match":66,"seq_b_single":39}
ttagccctaaacacaaataattacaaaaacaaaattatttgccagagtactaccggcaacagcccgaaactcttggcggtgctttataccctt
+
CCC?CCCCCCCCeeccebe^bS[_ZQYYbW`[cbD\aCEEEBDD?CB>BEBBEEDLEBLECEAECEEEFEEEEEEEEELEECCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:92:13586:15158#0/1_sub[74..81] {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:gcctcgt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcgt","obimultiplex_reverse_tag":"gcctcgt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":2854,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ccgatagg
+
R]VZ__dd
@HELIUM_000100422_612GNAAXX:7:1:8699:18900#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":89,"pairing_fast_score":0.674,"pairing_mismatches":{"(G:02)->(C:34)":105,"(G:27)->(T:02)":50,"(G:31)->(T:02)":23,"(T:02)->(A:34)":95,"(T:02)->(C:34)":108,"(T:02)->(G:30)":84,"(T:33)->(C:02)":29},"score":104,"score_norm":0.91,"seq_a_single":19,"seq_ab_match":81,"seq_b_single":19}
ccgaaggccctgttatccctaaggtagcttggtctttgatctattgttaggatcttttgagtgattaggtaatcttatttgtggtggatgatgaattccatggtcgccccaaccgaatggccttcgg
+
BBBBBB<B>B@@>B@B@B>DA@IB=DDDKDB@=DD@BD5DCDCBDD@@DE:8;:>4=@A4`MP???57EEEECAEE::C@?A9HC?AAE;EAEELEEELDDEEALEDLCB?C<??;;@@CCCC?CCC
@HELIUM_000100422_612GNAAXX:7:1:8726:19690#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttaggaa:gcgcgaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttaggaa","obimultiplex_forward_tag":"ttaggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcgaa","obimultiplex_reverse_tag":"gcgcgaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":63,"pairing_fast_score":0.85,"pairing_mismatches":{"(c:13)->(a:34)":73,"(c:32)->(g:02)":41},"score":1735,"score_norm":0.952,"seq_a_single":45,"seq_ab_match":60,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCBLDEEEEEEEEEEEEDDEDDBDBJ,D??C>CEEEBbY_]]UcZ^Ucac\c_a\Z_Ea\\`\Y^\CCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8645:10214#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":85,"pairing_fast_score":1,"score":5826,"score_norm":1,"seq_a_single":23,"seq_ab_match":85,"seq_b_single":23}
cctacacgacgctcttccgatctccgcgatgaggtcgccccaaccgaatcttagcatatttgtaatatgaatatgataaagctctatagggtcttcttgtcatcgcggagatcggaagagcggttcagcag
+
5<7:8941?8@AC?=?2A:)<BAU^[^[bZbd\\cc[cbcbaaccbeceeeeccefeeeeeeeedeeededefdbdeeeb]Zfccbdd__``c`bccce_c_bYYZYLBB#####################
@HELIUM_000100422_612GNAAXX:7:1:8816:16813#0/1_sub[75..81] {"ali_dir":"right","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttctaac:ttctaac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttctaac","obimultiplex_forward_tag":"ttctaac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttctaac","obimultiplex_reverse_tag":"ttctaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":4295,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
cctctaa
+
eebe_ee
@HELIUM_000100422_612GNAAXX:7:1:9224:2420#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacaggt:aacaggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacaggt","obimultiplex_forward_tag":"aacaggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacaggt","obimultiplex_reverse_tag":"aacaggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":62,"pairing_fast_score":0.864,"pairing_mismatches":{"(C:02)->(A:35)":59},"score":1345,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCCCCCCEEEEEEdb_aXc^ddN]bcTWY^]\[aAEDED>EEAEE@MEBFEEEEEEEECEEEEELDEEECCCCCCCCCCCCC@CCCCB
@HELIUM_000100422_612GNAAXX:7:1:8612:15682#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":107,"pairing_fast_score":0.923,"pairing_mismatches":{"(A:02)->(C:34)":98,"(A:02)->(T:34)":94},"score":4730,"score_norm":0.981,"seq_a_single":1,"seq_ab_match":105,"seq_b_single":1}
ccagcacatgcccattgagtctctgcacctatcctttttttctgtctttatggaactttttttgttttcggaaactaggatttggctcaggattgcccgcatgtgctgg
+
CcU_YZXYSdbL_dbdZbbeece^cccc`adaac^b_eeedda`eeeeeeefeddabdddddddddd\`cEEEEEEEEEEEEEEEEEEEEEEELEEELEEEEEEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:9261:15990#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (accaatc:accaatc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"accaatc","obimultiplex_forward_tag":"accaatc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"accaatc","obimultiplex_reverse_tag":"accaatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":3690,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ttagccctaaacacaagtagtcacaaaaacaagattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCACZW]dc_\eV_^]G^^O\b_V`Z]PWWUYV\_c__\c[XbLPMPQa^XX_bc]bdeeeL_`cSCCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8705:12573#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":106,"pairing_fast_score":0.816,"pairing_mismatches":{"(A:02)->(C:34)":97,"(A:02)->(T:34)":102,"(G:34)->(T:02)":12,"(T:02)->(G:34)":108,"(T:34)->(G:02)":11,"(T:34)->(G:22)":17},"score":4571,"score_norm":0.934,"seq_a_single":2,"seq_ab_match":99,"seq_b_single":2}
caccacgtactgcccattgagtctctgcacctatccctttttttttcgttttctgaatctttgttttaagaaaacaggatttggctcaggattgcccgcagtacgtggag
+
##EEEE?EEELLEEcaCdbTaec\^\\\YW^<[`QKX__beeeadb^ee_eee\ab]````c^ad[Xab`eee_`de`be_deR_baEEEAEEEEELEEELLEEEEEL##
@HELIUM_000100422_612GNAAXX:7:108:13514:11857#0/1_sub[28..127] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (attaact:aattaac)","obimultiplex_forward_error":2,"obimultiplex_forward_match":"taggataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"attaact","obimultiplex_forward_tag":"attaact","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aattaac","obimultiplex_reverse_tag":"aattaac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":61,"pairing_fast_score":1,"score":3739,"score_norm":1,"seq_a_single":47,"seq_ab_match":61,"seq_b_single":47}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCVYXe^]bb[eebdYWYV[`c]c^ZZSZXNPPQK``c`cZcc_Zb^adcc^`b`_cdcK_\\CCCBCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8702:18389#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":100,"pairing_fast_score":0.845,"pairing_mismatches":{"(A:33)->(G:02)":12,"(C:34)->(T:02)":36,"(G:34)->(A:02)":33,"(G:34)->(T:18)":58},"score":4270,"score_norm":0.96,"seq_a_single":8,"seq_ab_match":96,"seq_b_single":8}
tccgatctcccactgtgacccattgagtctctgcacctatcccgctttctaaactctggtttgttcgcgtaacccaggatttggctcaggattgcccgcatgagtgggagatcgga
+
########EEEKEEEEEEEEEDFEEEFEEEEELEELEE`YJeecfeededeeefeeeDdfeeeeeY_e^_ccceebbcRRRPQcZ___\Y\\^a_[caYMYXVZW[WS?#######
@HELIUM_000100422_612GNAAXX:7:1:8617:20259#0/1 {"ali_dir":"left","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":52,"pairing_fast_overlap":86,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:34)->(G:02)":47,"(A:35)->(G:02)":30,"(C:02)->(G:33)":108,"(C:34)->(G:02)":65,"(G:32)->(C:02)":55,"(G:34)->(T:02)":60,"(T:07)->(C:26)":95,"(T:32)->(C:02)":54,"(T:34)->(C:02)":38},"score":1551,"score_norm":0.884,"seq_a_single":22,"seq_ab_match":76,"seq_b_single":22}
ccagcacagctccgaaggcgtcaaggaacactgtgcctaaccggggacgcggctggcttgctggccgcaccttgggttgcaatgctatataatccacacgactctcggcaacggatatcactcgacgtgg
+
CCCCCCCCCCCCCCCCCCBCCCEEEDEEEME?EEEBLLECEEEEEELEFEC=BJJE>EAL3CECLCECEEDEBBbdabbRSSRSR^\ba`NATH?BOKMJW\D`Y]DKBBBBB>BA=@B@BBBBBBBBBB
@HELIUM_000100422_612GNAAXX:7:1:9246:2219#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (ttatgtg:ttatgtg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttatgtg","obimultiplex_forward_tag":"ttatgtg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttatgtg","obimultiplex_reverse_tag":"ttatgtg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":3153,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCCC`C]`WZZYWbcccVTKLWLYVVKbVc[[S\`ZZac_\e_cecbaa`e`eceeTaBEEEEEAECCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8846:2175#0/1 {"ali_dir":"right","ali_length":10,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":5,"pairing_fast_overlap":10,"pairing_fast_score":0.714,"pairing_mismatches":{"(C:33)->(A:34)":99,"(T:29)->(G:34)":108},"score":408,"score_norm":0.8,"seq_a_single":98,"seq_ab_match":8,"seq_b_single":98}
gtgaaataaaaaaaactgctcccaaaaacccgccgagccacagcttaaaactcaaaggacctggcggtgcttcatatccctctagaggagcctgttctacgccggcggtagataccccactatgcttagccctaaacctcaacagttaaatcaccaaaactgctcgccagaccacaccgagcaacagctacacactcacagaacat
+
###########################################@CCCCAAA69??3;?4@CCCC@CCC=CCBAAC?ACCCBC@CCCCCCCCCBCCCCCCcccab`ebCBDDBD<@CBCBDD?BBC@?<=@??=DD;@BA@B;@@<?3@##########################################################
@HELIUM_000100422_612GNAAXX:7:1:9249:10399#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (taatcat:taatcat)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"taatcat","obimultiplex_forward_tag":"taatcat","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"taatcat","obimultiplex_reverse_tag":"taatcat","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(T:33)->(A:02)":21},"score":1150,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaagctcaaaggacttggcggtgctttataccctt
+
CCCDCCCBCCCDBCCCCCCEKaccebd[dbaecaQVaY8=EEEDEEDEAEECAEBEBDEED?EEEEEEFEBEELEELEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:113:15348:16933#0/1_sub[78..81] {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcctcct:gcctccc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcctcct","obimultiplex_forward_tag":"gcctcct","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctccc","obimultiplex_reverse_tag":"gcctccc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":51,"pairing_fast_overlap":58,"pairing_fast_score":0.927,"pairing_mismatches":{},"score":4118,"score_norm":0.983,"seq_a_single":50,"seq_ab_match":57,"seq_b_single":50}
tttt
+
deef
@HELIUM_000100422_612GNAAXX:7:1:8626:3632#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4265,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaacaccgagatctacactctttccctacacgacgctcttccgatctccaccaatccaccaactctttcattcagacgtatacgtatgtatatacggaccattcggagatcggaagagcggttcagcaggaatgccgagacggatatcgtatgcg
+
################?>>??=6886>8358878?@A@AAAA8B@;ABCdedceeeeecadc`eeeeeeefdceeeedeefeeefefeeedeeeeedee`cZ^caY]a?A==>=?CCCC??=A?#################################
@HELIUM_000100422_612GNAAXX:7:1:8622:16076#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":92,"pairing_fast_score":0.91,"pairing_mismatches":{"(A:02)->(C:34)":102,"(C:33)->(G:02)":25},"score":2936,"score_norm":0.978,"seq_a_single":16,"seq_ab_match":90,"seq_b_single":16}
ccgcgcattattcggttggggcgaccgtggattaatcatcctcacctatatagatatatttatcatttactgaccctttaaaagatcatcaataaagctaccttagggataacagaatgcgcgg
+
CCCCCCCCCCCCCCCCEEEEEEEEKEEEEEEDEEaR]\_Tddd`Mecfcfc^dd_^ca\a]`WWaRRQORMOQRLZY[YZcdccEEEEEEEEEEEEEEEDDLEEEBEECCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8744:17174#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aaccaag:aaccaag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccaag","obimultiplex_forward_tag":"aaccaag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aaccaag","obimultiplex_reverse_tag":"aaccaag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":62,"pairing_fast_score":0.847,"pairing_mismatches":{"(C:02)->(G:26)":56},"score":1014,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCBBCACCC??`IbaaeedKYYWRT]@^bA=@EB>A;B>2222.4=8D;DEEEEDEEEEEEEBEEEEELAEELBCCBCCBCCCBCD:CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8827:17194#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtaggt:tgtaggt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtaggt","obimultiplex_forward_tag":"tgtaggt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtaggt","obimultiplex_reverse_tag":"tgtaggt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(a:02)->(c:34)":72,"(a:02)->(g:33)":77,"(a:17)->(c:30)":58,"(a:27)->(c:02)":43,"(a:32)->(t:02)":49,"(g:28)->(a:02)":45},"score":605,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacctcaacagttaaatcaacaaaactgctcgcaagaacactacgagccacagcttaaaactcaaaggacctggcggtgcttcatatccct
+
C@C@CCCACCCC@@;BBBABCBEA?C8CCCEEEBB?EB?:CCEDFA?CJ66@C]\UQ@Y\PYOVaaEB@EELDDD7KEEB><CC@C?BBC@CB?B7?CBC
@HELIUM_000100422_612GNAAXX:7:1:8686:8214#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":104,"pairing_fast_score":0.891,"pairing_mismatches":{"(C:02)->(A:34)":106,"(G:30)->(T:02)":10},"score":3041,"score_norm":0.971,"seq_a_single":4,"seq_ab_match":101,"seq_b_single":4}
atctccactgacgtcgggcaatcctgagccaaatcacgttttccgaaaacaagcaaaggttcagaaagcgaaaataaaaaaggataggtgcagagactcaatgggacgagat
+
####DDDDDHDDD>@@8@@DDDADDDDbbaZS_adaYaaddcc`__cdddd[[ba`[_[WX``a\SP][XITTTaaEEEEEEEEEEEEAEEEEEEFEEEELEEEELEE####
@HELIUM_000100422_612GNAAXX:7:1:8685:17406#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4281,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gctaccaccgagatctacactctttccctacacgacgctcttccgatctccatactcgcggatatccgttgccgagagtccttggcacatgccgtgccgcgctgatggagatcggaagagcggtcagcaggaatgccgagaccgatatcgtatgccg
+
#####AA>B+?<A7>52<'6::1>8/783699?<4<)<@CCCCC@?BCCeedfecbebedeeededeceeaceeeeedeecabeeeed_eedeeedebeee`efcZc`ACCC8ACC@CCCCDCC#################################
@HELIUM_000100422_612GNAAXX:7:1:8942:5060#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aaccgaa:aacggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aaccgaa","obimultiplex_forward_tag":"aaccgaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacggaa","obimultiplex_reverse_tag":"aacggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(t:33)->(a:02)":35},"score":1506,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCDCCCCCCCCCACELEEEEEEEEEDEEDKDEEBAE=E12111<???_^]YL[FUQQLYUW\\^b_\ceecWeEEE@A?AA=8DCCCCCCBCCBC
@HELIUM_000100422_612GNAAXX:7:1:8912:18241#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atgatca:atgatca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atgatca","obimultiplex_forward_tag":"atgatca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atgatca","obimultiplex_reverse_tag":"atgatca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":3290,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCELEEEa]UVb_^cRPIQR]]]dZQQORPVZZ\Y^b\^b`\cY[_a[^[aaa_``db_Xa\]XCCCCCCCCCCCCCDCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8664:19325#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":58,"pairing_fast_overlap":73,"pairing_fast_score":0.829,"pairing_mismatches":{"(A:32)->(C:06)":48,"(C:02)->(T:34)":100,"(T:02)->(C:34)":95},"score":2519,"score_norm":0.959,"seq_a_single":35,"seq_ab_match":70,"seq_b_single":35}
ccacgagcacagcacggcatgtgccaaggaaaacaataaacgagaagaactcgacctgttatgccccgtttgtggtgtgcattctgagcgtgtcctcctttgaatcacaaacgactctcggcaacggatatctgctgtcgtgg
+
CCCCCCCCCCCCCBCCCCCCCCCCCCCCBCCCCCCbad]Z\][RIRPER\[RSS]Y]]cccYXYaZdebe_cc\MccccFEEEEEDEEEDEEEELAEEELEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:19533#0/1 {"ali_dir":"left","ali_length":92,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":76,"pairing_fast_overlap":92,"pairing_fast_score":0.854,"pairing_mismatches":{"(C:02)->(T:34)":98,"(G:02)->(T:34)":102,"(T:02)->(C:34)":108,"(T:34)->(A:10)":32},"score":4042,"score_norm":0.957,"seq_a_single":16,"seq_ab_match":88,"seq_b_single":16}
ctaggatatccgttgccgagagtcgtgtggattatatagcattgcaacccagggtgcagccagcaagccagccacgtccccgggttaggcacagtgttccttgacgccttcggctagtgcgtgg
+
CCCCCCCCCCCCCCCCeeeaW[]^HQSJJYJFZZ``deca_T__aN\ee`X`McSSaZ`ebXbcaecc^b\]^^\^W]aaeeeaeEEEEEEEEEEEELEEELEEEEELCCCCCCCCCCCCCBCC
@HELIUM_000100422_612GNAAXX:7:1:8664:16481#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":100,"pairing_fast_score":0.639,"pairing_mismatches":{"(A:02)->(G:33)":84,"(A:02)->(G:34)":78,"(C:02)->(A:34)":102,"(C:34)->(A:02)":25,"(G:02)->(A:33)":76,"(G:02)->(A:34)":82,"(G:33)->(A:02)":12,"(T:02)->(C:34)":98,"(T:02)->(G:34)":105,"(T:34)->(A:02)":18},"score":1539,"score_norm":0.9,"seq_a_single":8,"seq_ab_match":90,"seq_b_single":8}
ccatcgcgagagggcaatcctgagccaaatccgtgttttgagaaaaaaaaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtctcgcgatgg
+
CCCCCCCCEEEKEEEEELDEEEEELEEEBEE`ccbc_baIRI_`cedddSGdeee`cEEEEEEEDEEEEDEEEFEKELEEELEKFEEEEEEEEEEEELEEELEELEFECCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8684:20977#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":48,"pairing_fast_overlap":89,"pairing_fast_score":0.558,"pairing_mismatches":{"(A:02)->(C:33)":106,"(A:02)->(T:33)":108,"(A:08)->(G:02)":50,"(A:15)->(T:02)":46,"(A:23)->(G:02)":62,"(A:31)->(C:02)":34,"(C:02)->(A:33)":95,"(C:29)->(G:02)":52,"(T:25)->(G:02)":57,"(T:28)->(A:02)":20,"(T:33)->(A:02)":38,"(T:33)->(G:02)":30},"score":278,"score_norm":0.865,"seq_a_single":19,"seq_ab_match":77,"seq_b_single":19}
ccttatgtgtacaaccaaatccaatttcatatcaatattaaaattaataatccaaataaccaaatgacaatgtaatccatcattccacttagaaacaagctgcaccttgacctgaaatcacataagg
+
@@@??8>>;;@BA@@BB@BFDDCDCB>D@K@@BI.DDKC:DB@AD92212@G)<@AC<6<2A5<92N@JP^^W^DCC<A<<1BB@@BB=<B9DDKD8B@@BBB=DKAKBBBBB><@<<@BB@B?BBB
@HELIUM_000100422_612GNAAXX:7:1:8642:1506#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":102,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:34)->(T:02)":16,"(T:02)->(G:34)":103},"score":3837,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagcactctcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggagagtgctgg
+
CCCCCCEEEEEEEEELEEEEEEd_M\TZWZbe]ca\b]XcZ_P_\]ebc\`\``[Zecbec`Z_`aeceecebZedec_]cEEEEEEEEEEEEEEEEEEEEELEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8662:18996#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":85,"pairing_fast_overlap":102,"pairing_fast_score":0.859,"pairing_mismatches":{"(T:02)->(G:34)":90,"(T:10)->(C:31)":65,"(T:14)->(C:02)":24,"(T:34)->(G:02)":8},"score":3445,"score_norm":0.961,"seq_a_single":6,"seq_ab_match":98,"seq_b_single":6}
ccagagctctgccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgcgtactctgg
+
CCCCCCELEEEEEDEEEEEEEEE8EEEEEEa^be^ZR^UcJ]ca`e__ebfXbbece]eXeaeaC`\cc\babeedbbcebcaEDEEEELEEEEEDEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9397:6721#0/1_sub[74..81] {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgccgg:ttcgagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgccgg","obimultiplex_forward_tag":"gcgccgg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcgagt","obimultiplex_reverse_tag":"ttcgagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":4474,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ccgatagg
+
eeeddeee
@HELIUM_000100422_612GNAAXX:7:1:8624:11064#0/1 {"ali_dir":"left","ali_length":58,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":33,"pairing_fast_overlap":58,"pairing_fast_score":0.6,"pairing_mismatches":{"(A:02)->(G:18)":82,"(C:02)->(G:34)":102,"(C:02)->(T:34)":108,"(C:09)->(T:32)":77,"(G:02)->(A:25)":87,"(T:02)->(G:32)":105},"score":611,"score_norm":0.879,"seq_a_single":50,"seq_ab_match":51,"seq_b_single":50}
ccacgagcgtccgacgcgaatcgcgtcaaggaacttgaacaagagaagagaagcgcgtgtgcccctgggttctcgttcgcgggtgaagctggggggcagcggtggcatcttttatgaacaacataacgactctcggcaacggatatcgcacatcgtgg
+
CCCDCCCCCCCCCCCCCCCCCBCCCCCCBCCCCCCC@CCCCCCCCA3CCC?A?ADBDDEDCDA@CCCBcNdce`ZVD`YX+<=@71C6DACEBAAA77L5ELEEJ?@LCCCCCCCC@CCBCCDCCCCCACCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:26:17564:3814#0/1 {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{"(T:31)->(G:23)":95},"score":3319,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ccgaatatctagaacacggctcctctagagggatgtaaagcaccgccaagtcctttgagtttcaggctgttgctagtagtactctggcgaacattcttgtttattgaatgtttatgtttagggctaagcatagtggggtatctaagatattcgg
+
CCBCCBCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEDEEEedeWebeccaeccef\]_`U`bbcbaa`eeecce^TTZQR@_cSa_ccc_aFEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8621:2974#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":6262,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
cctacacgactctctccgatctccgcgaacgcaagaagaccctatagagcttatattctattatattagcttaataaatagaattcggttggggcgacccgttcgcggagatcggaagagcggttcagca
+
###################?=>\aaac_dd`eededfbaeedefeeeebeeeeeeeedeedeeeeeeeeeeeeeefeeeeeeeeadebdeceedeeeeeeee_aeeba@CC4;A;>3@>:7A?518;<?;
@HELIUM_000100422_612GNAAXX:7:1:8628:6474#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":94,"pairing_fast_overlap":102,"pairing_fast_score":0.949,"pairing_mismatches":{"(C:02)->(G:34)":104,"(C:02)->(T:34)":107},"score":5407,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccagagtacgcgggcaatcctgagccaaatccatgttttgagaaaacaagggggttctcgaactagaatacaacggaaaaggataggtgcagagactcaatgggcgtactctgg
+
CCCCCCbPcce`ec]cce\eace_aeecbbadceedeeeee[bebeeb^deeec^eeeaebec]eY^Z^Y_cc__ad_cae\ecEEEEEEEEEEEEEEEEEEELEELECCCCDC
@HELIUM_000100422_612GNAAXX:7:1:8628:13866#0/1 {"ali_dir":"right","ali_length":75,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":72,"pairing_fast_overlap":75,"pairing_fast_score":1,"score":5262,"score_norm":1,"seq_a_single":33,"seq_ab_match":75,"seq_b_single":33}
acactctttccccacacgacgctcttccgatctccagatcgacgccattgagtctctgcacctatcggcaattggactacttggctcaggattgccccgtcgatctggagatcggaagagcggttcagcaggaatgcggag
+
##################?C@>CA+ABB523;8X\VLWaeTbeeeedeeeebeecec^cc_ccdceebeeeeeedeefeeeededcdeecaddbcfdee^beb]cecaBC@(6AA;)A>?#####################
@HELIUM_000100422_612GNAAXX:7:1:8699:8462#0/1 {"ali_dir":"left","ali_length":81,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":62,"pairing_fast_overlap":81,"pairing_fast_score":0.795,"pairing_mismatches":{"(C:02)->(G:34)":97,"(C:09)->(G:02)":58,"(G:33)->(A:02)":43,"(G:34)->(C:02)":57,"(G:34)->(T:02)":29,"(T:34)->(G:02)":30},"score":767,"score_norm":0.926,"seq_a_single":27,"seq_ab_match":75,"seq_b_single":27}
ccagagacgtggatatccgttgccgagagtcatattttttctgttgccttggccttgccaaccgacatagcccttgagcggctcgccagtgcctcagcttttatcgtgttccttggcgtcatcccgtacgtctgg
+
CCCCCCCCCCCCCCCCCCCCCCCCCCDELLEEEEEEEEDBDDKEEEEEE>E?EBEEL3BB=<-;::YY[@`cc]`^`Z^EBEEAABEEEE=CEEBELAECCEEAEDEECCCCCBCCCCCCCBCCCBCCCCACCCC
@HELIUM_000100422_612GNAAXX:7:1:8615:13215#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4325,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcgaccaccgagatctacactctttccctacacgacgctcttccgatctccgccggcgcaccaactctttcattcagacgtatacgtatgtatatacggggtacgtggagatcggaagagcggttcagcaggaatgcggagacagatatcgtatgcc
+
##############################C@CC>CAC>A;A>C?=CCCececeaeeeeddedeeeeeeefeeeeeeeedeeeeeedeeeeeeefedeeeebe__cccABA;8@>A;AA######################################
@HELIUM_000100422_612GNAAXX:7:1:8674:18915#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(A:10)->(C:34)":99},"score":5559,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccgaaggccttagataccccactatgcttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggccttcggagatcgcaagagcggttc
+
#####?<<;+>@?*0=A?[]X^]_`caUc[a_`_acee___c_bdadZdeeeedeeeeeebdbdedccdeedceeceec]`cdV^\]^[[][YRSORRFQRRRRRRRRA=?###############
@HELIUM_000100422_612GNAAXX:7:1:8657:18324#0/1 {"ali_dir":"left","ali_length":95,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":95,"pairing_fast_score":0.946,"pairing_mismatches":{"(A:02)->(C:34)":71,"(C:02)->(T:34)":108},"score":2586,"score_norm":0.979,"seq_a_single":13,"seq_ab_match":93,"seq_b_single":13}
tagatatccgttgccgagagtcgtgtggattaaatacgattgcaacacaagagatgaccagcaagctagccatgccctcgggttagacacagtgttccttggcgtcatcctacgtgcgtgg
+
CCCCCCCCCCCCCCEEEEEEEEaa`]Qa^R\ccbc\YW]\UYE\adaaddecaZbY\`[^_HaECBBBEELDCEEEEDEEEEEEEEEEEEEEEEEEEEEEEEEEEEELCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8618:19823#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (gcgcatt:gcgcatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgcatt","obimultiplex_forward_tag":"gcgcatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcatt","obimultiplex_reverse_tag":"gcgcatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":62,"pairing_fast_score":0.915,"pairing_mismatches":{},"score":2214,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCDBCCCCCCCCCCCCCCB``_]]]X\_acae]]][aITc_ac____XTLXVOZ_4CDAEDBDEEEEEEEEEEEEELDE?LCCBCCCCCCCCCBBCCCCB
@HELIUM_000100422_612GNAAXX:7:1:9154:5991#0/1 {"ali_dir":"right","ali_length":53,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":50,"pairing_fast_overlap":53,"pairing_fast_score":1,"score":3387,"score_norm":1,"seq_a_single":55,"seq_ab_match":53,"seq_b_single":55}
agatacggcgaccaccgagatctacactctttccctacacgacgctttccgatctccacggtactagaacaggctcctctagcatagtggggtatctaagtaccgtggagatcggaagagcggttcagcaggaatgccgagaccgatctcgtctgcggtcttt
+
#####################?77;776;5-95?<7-?:A=8ADCCCCCCB@CCCcaaYYRT\a\\^MWbd``d_`ZWYU`bdddddeddb[dcc]bbbbYdW`dd`b?@@>@B@################################################
@HELIUM_000100422_612GNAAXX:7:1:8684:3138#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":87},"score":4464,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccagcgagcgagggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtcgctcgctgg
+
CCCCCC\d`be`ee][a^Tc\ebcbdcd`N_eebc[bcbUcVSbaZeeZcSY\RQbcc]cbcW\aaeee]dcJ\dEEEEEEEEEEELEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8627:2710#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":89,"pairing_fast_score":0.86,"pairing_mismatches":{"(A:02)->(C:34)":90,"(C:34)->(A:32)":25,"(G:34)->(A:13)":29},"score":3704,"score_norm":0.966,"seq_a_single":19,"seq_ab_match":86,"seq_b_single":19}
ccttccgctattcggttggggcgaccaaggaataaatcatccttacaccataagacccattgatctttataaagaccctaataatagacctaaagataagctaccttagggataacagagcggaagg
+
CCCCCCCCCCCCCCCCCCCa_a]cCcYcEccaQQRQJ_]_`cbccddUb`edbcLa`db`c_b\cbcccceeeedcaccEEEEEEEEEELEEEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8610:15615#0/1 {"ali_dir":"right","ali_length":106,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":87,"pairing_fast_overlap":106,"pairing_fast_score":0.845,"pairing_mismatches":{"(A:31)->(G:16)":44,"(A:34)->(G:02)":18,"(C:02)->(T:30)":98,"(G:34)->(A:02)":10},"score":3010,"score_norm":0.962,"seq_a_single":2,"seq_ab_match":102,"seq_b_single":2}
ctccagatcgcgagggcaatcctgagccaaatccttctttttgacaaacaaataaagattccgaaagaaataagggataggtgcagagactcaatggtcgcgatctggag
+
##EEEEEEELEEEEEEELEEEEEEEEEEEEEEEEEEEEEa_`XA[K\\`Y^`__a]\\Tcbc[_eedeedddaeeca^^^[`YXSVYcEEEEEEEEEHEEEEDEEEEE##
@HELIUM_000100422_612GNAAXX:7:1:9070:3610#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aacaaca:aacaaca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacaaca","obimultiplex_forward_tag":"aacaaca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacaaca","obimultiplex_reverse_tag":"aacaaca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":63,"pairing_fast_score":0.917,"pairing_mismatches":{"(a:06)->(g:34)":76},"score":1216,"score_norm":0.968,"seq_a_single":45,"seq_ab_match":61,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
CCCCCCCCCCCCCCCCCCLEEEEEEEEEEEEEDDFDDDDAEEECCE@EEACCE?<???A?<_`]]ac_d^c_N^WGZ[cbcDCCCCC?CCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8644:20031#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(A:02)->(G:34)":94,"(T:34)->(G:05)":35},"score":4915,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
cccactgctgtccattgagtctctgcacctatcctttttttattctccctttctgaactcttcttggttttcggaaaacaggatttggctcaggattgcccacagcagtggg
+
CCCCEEEcc__T[[QY[cbbeceabea`^^^\ceH`Z_ecdbcddeceeeeeeg`aebeeeceeeebaeeecabceeeeFEEEEEEEEEEEEELEEEEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:9196:10266#0/1_sub[74..81] {"ali_dir":"right","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (actaagt:actaagt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"actaagt","obimultiplex_forward_tag":"actaagt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"actaagt","obimultiplex_reverse_tag":"actaagt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":59,"pairing_fast_overlap":62,"pairing_fast_score":1,"score":1678,"score_norm":1,"seq_a_single":46,"seq_ab_match":62,"seq_b_single":46}
ccgatcta
+
_[UbaY;8
@HELIUM_000100422_612GNAAXX:7:1:8668:16509#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":102,"pairing_fast_score":0.899,"pairing_mismatches":{"(G:02)->(T:34)":99,"(T:34)->(G:02)":8,"(T:34)->(G:17)":42},"score":4253,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
cccacactagcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgctagtgtggg
+
CCDCCCELEDEEEEEEEEEEEEEEEEEEEeee[eecae_acDcdc`\__cZbdeb_fedeede]ddeedceddee\_ccc``\Q\^_ac_ecEEEBEELEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8644:21235#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":102,"pairing_fast_score":0.838,"pairing_mismatches":{"(A:02)->(G:34)":96,"(C:34)->(N:02)":40,"(T:34)->(N:02)":43},"score":3154,"score_norm":0.902,"seq_a_single":6,"seq_ab_match":92,"seq_b_single":6}
ccatgtgcagcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgcccgctgcacatgg
+
CCCCCCEEEEEEEEEEEEEEEEEEEEEEEEEEEELLLLLLLLLEdeeeefb_feXfeedeebecd^aeceeddeceeeeeZ`N[Ma`ccPaEEEELEEEEFEEEEEEECBBBCC
@HELIUM_000100422_612GNAAXX:7:1:8672:14053#0/1 {"ali_dir":"right","ali_length":12,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":8,"pairing_fast_overlap":12,"pairing_fast_score":0.889,"pairing_mismatches":{"(C:34)->(G:34)":97},"score":713,"score_norm":0.917,"seq_a_single":96,"seq_ab_match":11,"seq_b_single":96}
cgagagtctgtgtgattatatagcattgcaacccaaggggcggccagcacgccagccgcgtccccgggttaggcagagtgttccttgacgccttcgscactgcagtggatatccgttgccgagagtcgtgtggattatatagcattgcaacccaaggtgcgcccagcaagccagcagcgtccccgggttaggcggcgcgttcct
+
################################################A0??@?C?ACC?C?CCCB@CC;?@C==CCCBCCCCCBC=CCCBBCABCCeeee`eeeee^>CBCCCCC@CCCCCCCB@CBCCBCC/=?C@CCC<<@A>C8C:CC9C9C################################################
@HELIUM_000100422_612GNAAXX:7:1:8642:18116#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":86,"pairing_fast_score":0.988,"pairing_mismatches":{"(C:02)->(G:34)":108},"score":4950,"score_norm":0.988,"seq_a_single":22,"seq_ab_match":85,"seq_b_single":22}
ctacacgactctcttccgatctccgaggcttcaagaagaccctatagagcttaatttaacctactaaataagtaaaaattaaattcggttggggcgaccaagcctcggagatcggaagagcggttcagca
+
?6;???90/,//0000(0?AB?^\]^^Y[TS]VYWMYL``cZccdeee_eaebebcbecc__cc]ccccce^Xddccecccecbc^ea`_b`ccccbEEEEEEEEEEL######################
@HELIUM_000100422_612GNAAXX:7:1:8636:11943#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":98,"pairing_fast_score":0.958,"pairing_mismatches":{"(T:34)->(G:08)":35},"score":4873,"score_norm":0.99,"seq_a_single":10,"seq_ab_match":97,"seq_b_single":10}
ccagctctgacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccctttctttgttttctcaaaacacggatttggctcaggattgcccgtcagagctgg
+
CCCCCCCCCCEEEeZ__d__eee_^c_\ca_a]aFeee]eUadeeeeed_cb`caddb`eeebcbdedbd^`ceddde]dcc[cEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8648:8149#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":66,"pairing_fast_overlap":73,"pairing_fast_score":0.943,"pairing_mismatches":{"(A:34)->(G:18)":66},"score":2432,"score_norm":0.986,"seq_a_single":35,"seq_ab_match":72,"seq_b_single":35}
ccacgtacgctgatatccgttgccgagagtcgtttgtgattcaaaggaggacacgctcagaatgcacaccacaaacggggcataacaggtcgagtccttctcatttattgttttccttggcacatgccgtgcagcgtacgtgg
+
CCCCCCCCCCCCCCCCCCCCCBCCCCCCC>CCCC<b`^aKI[bae^bcZMb_`eebb^`_d_R[`DebeX`aaaBAEEB??BDEEEE9EBEEEEEEE?EEDEEDDE@@C?CC@CCC@CCC>CCCCCBCCCCCCCCCCCBCCCC
@HELIUM_000100422_612GNAAXX:7:1:8622:17714#0/1 {"ali_dir":"right","ali_length":77,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":68,"pairing_fast_overlap":77,"pairing_fast_score":0.919,"pairing_mismatches":{"(A:02)->(G:34)":108,"(A:34)->(G:10)":45,"(C:34)->(T:15)":32},"score":3666,"score_norm":0.961,"seq_a_single":31,"seq_ab_match":74,"seq_b_single":31}
actctttccctacacgacgctcttccgatctcctatctcattagataccccactatgccctatctcattagataccccactattcgccagagtactacctgagataggagatcggaagagcggttcagcaggaatgcgg
+
6=?A5AA>00/(/00''/A?AA8000--A8ADa]]][\RRRQQHFZWZeecba`eccd\]\[Ueec^]eebcdececeedeedeedebe_eEEAEDEEEEEEEEEEEL###############################
@HELIUM_000100422_612GNAAXX:7:1:8934:5610#0/1_sub[28..126] {"ali_dir":"left","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (ttcaacg:ttcaacg)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"ttcaacg","obimultiplex_forward_tag":"ttcaacg","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"ttcaacg","obimultiplex_reverse_tag":"ttcaacg","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":58,"pairing_fast_overlap":63,"pairing_fast_score":0.967,"pairing_mismatches":{},"score":1314,"score_norm":0.984,"seq_a_single":45,"seq_ab_match":62,"seq_b_single":45}
ttagccctaaacatagataattttacaacaaaataattcgccagaggactactagcaatagcttaaaactcaaaggacttggcggtgctttatatccct
+
C?CBCCCCCCCCCCA?;BQ[\ebaebVX_WbZ_MANHZ^a8EEDEEC????:?C?C;<AA4DEEEDEEEEEEEEEEDEELECC?CC@CCCCCCC@BCCB
@HELIUM_000100422_612GNAAXX:7:1:8698:15270#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":98,"pairing_fast_score":0.874,"pairing_mismatches":{"(A:02)->(C:34)":97,"(G:34)->(A:08)":51,"(T:34)->(A:02)":15},"score":3421,"score_norm":0.969,"seq_a_single":10,"seq_ab_match":95,"seq_b_single":10}
ccacagacgcaccattagtctctgcacctatccttttcctttgtattctagttcgattcgagaacccctttgttttctcaaaacacggatttggctcaggattgccctgcgtctgtgg
+
CCCCCCCCCCEEEALEEEEEEEEEEEEEEEEEc_caaKc\\cSWWPZZa\F]^`]beedeeeebdb^bddeeded^daXceaceeEEEEEEEEEEELEEEDEEEEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8610:16294#0/1 {"ali_dir":"left","ali_length":98,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":84,"pairing_fast_overlap":98,"pairing_fast_score":0.884,"pairing_mismatches":{"(A:02)->(G:34)":100,"(C:02)->(T:34)":103,"(C:08)->(A:28)":60},"score":4949,"score_norm":0.969,"seq_a_single":10,"seq_ab_match":95,"seq_b_single":10}
ccacgtcgtacccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccctttctttgttttctcaaaacacggatttggctcaggattgccctagtgcgctgg
+
CCCCCCCCCC___]a[U`X_``_Y\^^QbbddLd`cadebecedcd]eef_`bdVac__@cedad^db_cdddedddedcXd_ccEEEEEEEEEEEEEELEELEEEEECCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8679:8708#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":4437,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacaccacgctcttccgatctccacagctgacccattgagtctctgcacctatcctttttgattttaacttaaggatttggctcaggattacccgtcagctgtggagatcggaggagcgcttcagcaga
+
1376=?A>'=='=AC=C9C6CCCC]eeeaR\`Rcc_ccecbdaeeeeceeeeeeeebeeeeeeedeeeeeedfeecebeeeceaaaEEEEEEEEEEEEEEEEEEEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8673:18307#0/1 {"ali_dir":"left","ali_length":76,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":76,"pairing_fast_score":0.753,"pairing_mismatches":{"(A:34)->(G:02)":42,"(C:02)->(A:34)":101,"(C:02)->(T:34)":103,"(C:08)->(G:02)":70,"(C:34)->(G:02)":44,"(G:29)->(C:02)":69,"(G:34)->(C:02)":45},"score":390,"score_norm":0.908,"seq_a_single":32,"seq_ab_match":69,"seq_b_single":32}
ccacgcagctcgcacggcatgtgccaaggaatactcataggagcgtcctctctcatcgccccgttctcgccgtgcgatgggcgaaggcacgcctctccaaagtctaaacgactctcggcaacggatatcgcgtagcgtgg
+
CCCCCCCBCCCCCCCCCBCCCCCCCCCCCCACEEEDEEEADL@LL9>AA<EEED?BEABEEEE=BCCAG29UODJ^IS`_C:C=DECBBEBAFEEBEEEBLELEEEEECC@CCCCCCCCCCCCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8741:11336#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (acaacaa:acaacaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acaacaa","obimultiplex_forward_tag":"acaacaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acaacaa","obimultiplex_reverse_tag":"acaacaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":45,"pairing_fast_overlap":62,"pairing_fast_score":0.763,"pairing_mismatches":{"(T:31)->(G:02)":21},"score":611,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ctagccttaaacacaaatagttatgcaaacaaaactattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCC?CCB@CCACCCCCCCCDIEEBB?>A_c_\U?PUYKQX><??C9?:7,988A?;?CBE=CEELEEEBEDEELEELEDDLC@CBCCCBCCCCBBCCCCC
@HELIUM_000100422_612GNAAXX:7:97:3453:2138#0/1_sub[29..128] {"ali_dir":"left","ali_length":61,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (cctcctt:gcctcct)","obimultiplex_forward_error":1,"obimultiplex_forward_match":"taagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"cctcctt","obimultiplex_forward_tag":"cctcctt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcctcct","obimultiplex_reverse_tag":"gcctcct","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":50,"pairing_fast_overlap":61,"pairing_fast_score":0.862,"pairing_mismatches":{"(T:30)->(G:24)":51},"score":2353,"score_norm":0.967,"seq_a_single":47,"seq_ab_match":59,"seq_b_single":47}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
AC<ABBBCCC@CCCCC@?@\T_cZ^aT`eRba_VUS__H[S_Xec_eUXS?LVa]SV_UcaBEEEEBBEECEE=EELAE-CCCCCCDCCCCCAA:BBCCA
@HELIUM_000100422_612GNAAXX:7:1:8662:18178#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(A:29)->(C:09)":32,"(C:02)->(T:34)":82},"score":3476,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
atcgccacagtcgaggggcaatcctgagccaaatcctgaaacaaataaaagttcagaaagtgaaaataaaaaaaggataggtgcagagactcaatggctcgactgtggagag
+
####DDDDDBCDBD=YK^^bb_`QLHOH[`MA`^Pb]HN^b]YY]_b`_QXRZ]ZYSQX_VUYV__aa\VZYZU_[b`EEELEEEBCEEDBEEEEECEEEEECEEEEE####
@HELIUM_000100422_612GNAAXX:7:1:8697:5122#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":102,"pairing_fast_score":0.939,"pairing_mismatches":{"(G:34)->(T:06)":40,"(G:34)->(T:09)":42},"score":3436,"score_norm":0.98,"seq_a_single":6,"seq_ab_match":100,"seq_b_single":6}
ccatgctgctggggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggcagcagcatgg
+
CCCCCCEEEEEEEEEEEEEEEEEEEcc_bdbb]^]XY_`GcFN`Pbc\]UdeeV_eebYabebab`cUdbc`cUcW[Z^S^EEEEEEEEEEEEEDEDEEEEEEEEEEECCBCCC
@HELIUM_000100422_612GNAAXX:7:1:8615:16494#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":103,"pairing_fast_score":0.88,"pairing_mismatches":{"(A:02)->(G:34)":95,"(C:02)->(G:34)":89,"(G:02)->(C:34)":101},"score":4538,"score_norm":0.971,"seq_a_single":5,"seq_ab_match":100,"seq_b_single":5}
ccacgagcacaccattgagtctctgcacctatccttttcctttggattctagttcgagaaccccttgttttctcaaaacacggatttggctcaggattgcccgtgtgcactgg
+
CCCCCEEEEEEEEcI_\aacbee]X`^[__```ed]ceeeaee][]U]^_^\ddceeeebeaeddcebeebde`acccccEEEEEEEELEEEEELEEEEELEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:9079:12932#0/1_sub[77..81] {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (atctgca:atctgca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctgca","obimultiplex_forward_tag":"atctgca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctgca","obimultiplex_reverse_tag":"atctgca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4325,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
agagg
+
feeed
@HELIUM_000100422_612GNAAXX:7:1:9063:17302#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tgtacag:tgtacag)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtacag","obimultiplex_forward_tag":"tgtacag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtacag","obimultiplex_reverse_tag":"tgtacag","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":46,"pairing_fast_overlap":62,"pairing_fast_score":0.78,"pairing_mismatches":{"(C:02)->(A:34)":59,"(C:34)->(G:28)":30},"score":1692,"score_norm":0.935,"seq_a_single":46,"seq_ab_match":58,"seq_b_single":46}
ttagccctaaacacaaataattatataaacaaaattattcgccagagtactaccggcaacagcccaaaactcaaaggacttggcggtgcttcacaccctt
+
CCCCCCCCDCCCCCCCCCDeeefee`^`]Cced]I_d^ca__c_dcEEC;BE>DCDEALEDEEEEEEEEEEEEEEBLCEELCCCCCCCCCCCACCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9381:2020#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aactatt:aactatt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aactatt","obimultiplex_forward_tag":"aactatt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aactatt","obimultiplex_reverse_tag":"aactatt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1377,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCCCCBAddccaecd^eeeeec\]dcAAECE?EEFEEEECCEEDEEEEEFEEEEEEDEEEEEEELCEEECCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8632:20786#0/1 {"ali_dir":"right","ali_length":90,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":90,"pairing_fast_score":0.954,"pairing_mismatches":{"(G:34)->(C:06)":32},"score":3698,"score_norm":0.989,"seq_a_single":18,"seq_ab_match":89,"seq_b_single":18}
acgacgctcttccgatctccacaacaaggtagtactctggcgaatagttttgtttgcataactatttgtgtttaaggctaggcatagtggggtatctaattgttgtggagatcggatgagcggttc
+
AA+88866653AA=:0AARcccc]]\^SZWZGVbe_deeeeeWbec``deeebabadeee`eaedbeeededeEEEBEEEEEEEEEEEEAEEEEDEEEEEEEEEEEEE##################
@HELIUM_000100422_612GNAAXX:7:1:8700:10365#0/1 {"ali_dir":"left","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":104,"pairing_fast_score":0.921,"pairing_mismatches":{"(C:02)->(G:27)":94,"(G:34)->(T:02)":8},"score":4430,"score_norm":0.981,"seq_a_single":4,"seq_ab_match":102,"seq_b_single":4}
cccgacagctgccattgagtctctgcacctatcctttttttattctccctttctgaactcttcttggttttcggaaaacaggatttggctcaggattgccccagctgtcggg
+
CCCCEDELEEEDE`TZRW``bZbWYb]Vc`caeUT__UaYWYS\[JbdeeZ_bYb\^c_ddbeeM`KY^^\`cTc[X^^`TL\VYXaEEDEEEEEBAEEEEEEEEEEECCCC
@HELIUM_000100422_612GNAAXX:7:1:8678:19780#0/1_sub[79..81] {"ali_dir":"right","ali_length":57,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (agcagag:tgacatc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agcagag","obimultiplex_forward_tag":"agcagag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgacatc","obimultiplex_reverse_tag":"tgacatc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":54,"pairing_fast_overlap":57,"pairing_fast_score":1,"score":4212,"score_norm":1,"seq_a_single":51,"seq_ab_match":57,"seq_b_single":51}
tgg
+
eee
@HELIUM_000100422_612GNAAXX:7:1:8686:18325#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (aacgagc:agccata)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacgagc","obimultiplex_forward_tag":"aacgagc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agccata","obimultiplex_reverse_tag":"agccata","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":57,"pairing_fast_overlap":62,"pairing_fast_score":0.966,"pairing_mismatches":{},"score":3615,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCDCCCCCCCCCCCCCC@>BKDDeeebccedc`YGY[[Z]]Kacaa^\`_]\eab`^`c]\_adac[_Y\YY[QZYQY`aUCDCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8700:12213#0/1 {"ali_dir":"left","ali_length":107,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":89,"pairing_fast_overlap":107,"pairing_fast_score":0.856,"pairing_mismatches":{"(A:02)->(G:34)":82,"(C:02)->(A:32)":100,"(T:02)->(G:34)":103},"score":4163,"score_norm":0.963,"seq_a_single":1,"seq_ab_match":103,"seq_b_single":1}
ccacgcgatcggggcaatcctgagccaaatccgtgttttgagaaaacaaggaggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatggtcgatg
+
Ceeb]^`[ZVIZTK``]ZbeY`ae[\\P^cZb`d[^[]^SaO[dZRbbYYY[UIOdbcUd_^QOceeb`abEEECLEEEEELEEEEEEEEBEECDDCCCJEELEEEEEC
@HELIUM_000100422_612GNAAXX:7:1:8643:7059#0/1 {"ali_dir":"right","ali_length":64,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":61,"pairing_fast_overlap":64,"pairing_fast_score":1,"score":4428,"score_norm":1,"seq_a_single":44,"seq_ab_match":64,"seq_b_single":44}
caccgagatctacactctttccctacacgacgctcttccgatctagcagggcaatcctgagccaaggcacacagggataggtgcagagactcaatggtgctcgtgtggagatcggaagagcggttcagcagaagtgcggagaccgacatcgc
+
###############################AA6B?A8C?A6?BeeeeZY^\]ZeebcdbcebcebeebebeeecedZ]ea^ddeeaeeeaeea^][Taccee]eaeXA>>0>A:?<AA#################################
@HELIUM_000100422_612GNAAXX:7:1:8674:12525#0/1 {"ali_dir":"right","ali_length":58,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":55,"pairing_fast_overlap":58,"pairing_fast_score":1,"score":4227,"score_norm":1,"seq_a_single":50,"seq_ab_match":58,"seq_b_single":50}
cgcgaccagcgagatctacactctttcccttcacgacgctcttccgatctccggaggaaccgtatatacatacgtatacgtctgaatgaaagagttggtgcggcgttgagatcggaagagcggttcagcaggaatgccgagacagatatcgtatgcgg
+
##################B:==B/8977?3)<;CB@CCCCC>CCCBBCCCefecaccUeeeeeeeeeeeeeceeeeeeeeeeaeededeeeeeefeecebeeaeeebc@CB8<@?A=BB#######################################
@HELIUM_000100422_612GNAAXX:7:1:8623:16956#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":100,"pairing_fast_overlap":104,"pairing_fast_score":0.99,"pairing_mismatches":{"(C:02)->(G:34)":108},"score":3752,"score_norm":0.99,"seq_a_single":4,"seq_ab_match":103,"seq_b_single":4}
atctccacgcatcgagggcaatcctgagccaaatcctgaaacaaataaaagttcagaaagtgaaaataaaaaaaggataggtgcagagactcaatggtcgatgcgtggagat
+
####EEEEEEEEEEEDEEEEEEBEaee\aa```WeceVZZc[Tdadeedadaac\c_a]eadaaddddddddd[^Qe^eEEEEEEEEEEEDEEEEEEEEEEEEEEDEL####
@HELIUM_000100422_612GNAAXX:7:1:8801:3204#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agccata:agccata)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agccata","obimultiplex_forward_tag":"agccata","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"agccata","obimultiplex_reverse_tag":"agccata","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":47,"pairing_fast_overlap":62,"pairing_fast_score":0.797,"pairing_mismatches":{"(A:02)->(C:34)":54,"(T:26)->(A:10)":35},"score":1150,"score_norm":0.952,"seq_a_single":46,"seq_ab_match":59,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagtccgaaactcaaaggacttggcggtgctttataccctt
+
CCC@CCCBCCCCCCCCCCCdVbddcddcTZWYdV>_c`A>AB98:89@CEEDELEEEEDEEDEEEEEEEEEEEEEELEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8624:17082#0/1 {"ali_dir":"left","ali_length":73,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":70,"pairing_fast_overlap":73,"pairing_fast_score":1,"score":2259,"score_norm":1,"seq_a_single":35,"seq_ab_match":73,"seq_b_single":35}
cccacagacgtgcacggcatgtgccaaggaaaacaacatattagaaggatgcatcctgctttgtcccgttcgcggtgtgcatacaggatgcggcctccttgaaatcacaaacgactctcggcaacggatatcacgtctgtggg
+
CCCCCCCCCCCCCCCCCCDCCCBCCC?CCCCCCCCd^eQRRNLXPaQ]_RWWVWHRYVBMTLRPITaQ[_]^]UU`aW[?@C9BEAEEEEEBEBEEEEBEEEED@EEEDCCCCCCCCCCCCCCCCCCCCCCCBCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8694:17499#0/1 {"ali_dir":"right","ali_length":104,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":101,"pairing_fast_overlap":104,"pairing_fast_score":1,"score":4432,"score_norm":1,"seq_a_single":4,"seq_ab_match":104,"seq_b_single":4}
atctccactgcagtggggcaatcctgagccaaatcctattttccaaaaacaaacaaaggcccagaaggtgaaaaaaggataggtgcagagactcaatggcactgcagtagat
+
####::A@>CDDD?DCDDDA[`Q]Z][Y^]Z^Z]S\SY\^`a^^[T[ca[ZWY[c`ZZYdfc`daa^NU``]][dd^`[^][O[`]Z``YcEEEE?EEEEEEEEEEAD####
@HELIUM_000100422_612GNAAXX:7:1:8620:11968#0/1 {"ali_dir":"left","ali_length":99,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":96,"pairing_fast_overlap":99,"pairing_fast_score":1,"score":5652,"score_norm":1,"seq_a_single":9,"seq_ab_match":99,"seq_b_single":9}
ccacgactagcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggctagtcgatctgg
+
CCCCCCCCC^ebeUeeeebc^ce[ceecee`deeeceebcedbedeedbddceebeeea_cd_bcdeeeaeceee^bccce`be`aIYaEEEEEEEEEEEEEEBEEEECCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:9059:18797#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (aacggaa:aacggaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"aacggaa","obimultiplex_forward_tag":"aacggaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"aacggaa","obimultiplex_reverse_tag":"aacggaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":41,"pairing_fast_overlap":62,"pairing_fast_score":0.695,"pairing_mismatches":{"(A:02)->(G:19)":58,"(C:02)->(G:09)":42,"(C:02)->(G:10)":53,"(T:32)->(A:22)":21},"score":895,"score_norm":0.919,"seq_a_single":46,"seq_ab_match":57,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcggcagagtactagcggcgatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CBC<CCCACCCCCCCCB=CaA_[cee`S_X[`TSKH^33:23@DBDB<5==848>)==@7?7CB?BB?=@3?EEEEICEEB@CACAC?CCCC<<399@B@
@HELIUM_000100422_612GNAAXX:7:1:8688:11666#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":98,"pairing_fast_overlap":102,"pairing_fast_score":0.99,"pairing_mismatches":{"(G:34)->(T:02)":7},"score":5367,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccatgtgcagcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaagaggataggtgcagagactcaatggcgctgacatgg
+
CCCCCCLEEdedeeacae`cbbccaebeeeefeeeeeeeeebbeedeebdeeeedeedfbcecUdeeee]__`Zc_c`ddbabbEEEEEEEEEEEEEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8634:5760#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":95,"pairing_fast_overlap":102,"pairing_fast_score":0.96,"pairing_mismatches":{"(A:02)->(G:34)":96},"score":5811,"score_norm":0.99,"seq_a_single":6,"seq_ab_match":101,"seq_b_single":6}
ccatcgtcagcccattgagtctctgcacctatccttttcctttgtattctagttcgagaacctccttgttttctcaaaacacggatttggctcaggattgcccgacgcagatgg
+
CCCCCC]WW]ecb`YcRec_cc]_acaccddeeeaa___aab`eeeeebde`eeddeceedbeedddeeeeddedceeeeedeUaccdcEEEEEELEEEEEEEEEEEECCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8896:9503#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (acggtac:acggtac)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"acggtac","obimultiplex_forward_tag":"acggtac","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acggtac","obimultiplex_reverse_tag":"acggtac","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(g:02)->(t:34)":81},"score":878,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCCCCCELECDEEFEFEDEEDDEEEABC=EECa\WR]]`ab]I`SW]@=EEE>EEEEBEEEEAEEEBL=?BB@C@C@C@>><A:C?C
@HELIUM_000100422_612GNAAXX:7:1:8668:14972#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":102,"pairing_fast_score":0.747,"pairing_mismatches":{"(C:02)->(G:34)":96,"(C:18)->(T:34)":54,"(G:02)->(C:34)":102,"(G:34)->(T:23)":17,"(T:02)->(G:34)":108,"(T:34)->(G:18)":41,"(T:34)->(G:28)":22},"score":4361,"score_norm":0.931,"seq_a_single":6,"seq_ab_match":95,"seq_b_single":6}
ccagcacgacaccattgagtctctgcacctatccttttcctttgtattctagttcgagaacccccttgttttctcaaaacacggatttggctcaggattgccctgtcgtgctgg
+
CCCCCC_da`_W__`WC\]c]CTZ__cN]c`c`a^P[\H[DVcc`cc`caa]]DU[]a^ae[]ebb]ebcbV`ec`X`Za``aEBEEEEECEDEELEEEEELEEEEELCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8676:3252#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":85,"pairing_fast_score":0.988,"pairing_mismatches":{"(C:34)->(A:28)":24},"score":5867,"score_norm":0.988,"seq_a_single":23,"seq_ab_match":84,"seq_b_single":23}
cctacacgacgctcttccgatctccagcagagggtcgccccaaccgaatttttattgggttgaccccaatttaaaataaagctctatagggtcttcttgctctgctggagatcggaagagcggttcagcag
+
#########C=AAC9>AC6?AAACeeeeecebadc^dce\eeeeaced`\eebedddeedeeeeeeeeeeecddeeeedbb]deZeee_c`\ceeceecU_ccYY^^`B@<@,00000<<::-<?39?@8A
@HELIUM_000100422_612GNAAXX:7:1:8692:14862#0/1 {"ali_dir":"left","ali_length":103,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":103,"pairing_fast_score":0.93,"pairing_mismatches":{"(A:02)->(T:32)":98,"(G:02)->(C:34)":101},"score":4766,"score_norm":0.981,"seq_a_single":5,"seq_ab_match":101,"seq_b_single":5}
ccacgagagagccattgagtctctgcacctatccctttttattctagtctagtttttaaacccttgtttttctaaaaataaagatttggctcaggattgcccctctctcgtgg
+
CCCCCeZeee_ZeX__XTQQQQRZc_^aeea_ec^W^]Lc_[baedaaeb]bb_eceebbcdd__bbdeebdcccdeeEEEBEECEEEEEEEEEEEEJEELEEEEEEECCCCC
@HELIUM_000100422_612GNAAXX:7:1:8620:13407#0/1 {"ali_dir":"right","ali_length":97,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":88,"pairing_fast_overlap":97,"pairing_fast_score":0.936,"pairing_mismatches":{"(G:34)->(A:02)":16,"(T:02)->(G:34)":107},"score":4215,"score_norm":0.979,"seq_a_single":11,"seq_ab_match":95,"seq_b_single":11}
tcttccgatctcccagcatgaggggcaatcctgagccaactcctttcttcaaaaggaaaaaaaagaataaaaaaggataggtgcagagactcaatggctcatgctgggagatcggagga
+
###########EEDELEEEde]da_eeccS`]`c_]a_^^aU\`Ueaeeeaeeeedadddde``__\]YYd````^YZYZSZaEEEEEEEEEEFEEEDEEEEEEEELE###########
@HELIUM_000100422_612GNAAXX:7:1:8676:20675#0/1 {"ali_dir":"right","ali_length":59,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":56,"pairing_fast_overlap":59,"pairing_fast_score":1,"score":4322,"score_norm":1,"seq_a_single":49,"seq_ab_match":59,"seq_b_single":49}
gcggccaccgagatctacactctttccctacacggcgctcttccgatctccagccatacaccaactctttcattcagacgtatacgtatgtatatacgggctcgttggagatcggaagagcggttcagcaggaatgcggagacagatatcgtatgcg
+
########?>6???63?A@A;A7<?<?;6;<9?()<.:8A?@@C@BCCCefeeeeeeceeeedeeeeffeeeeeeeeeeedeeaeedeeedeeeedddeedeee___a?C@8@@AB8AA######################################
@HELIUM_000100422_612GNAAXX:7:1:8628:18063#0/1 {"ali_dir":"right","ali_length":84,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":81,"pairing_fast_overlap":84,"pairing_fast_score":1,"score":5348,"score_norm":1,"seq_a_single":24,"seq_ab_match":84,"seq_b_single":24}
ccctacacgactctcttccgatctccggaggaaggtcgccccaaccgaatcttttattaattttattagttatagataaaactctatagggtcttcttgttcctccggagatcggaggagcggttaagcaga
+
#############AAA+6?A?B;=a_c`ZQVJV]ebeec\ccSeeceededdeeeefbaadeeeeebbeedeeeebeeedaaeaYYZZY[^Z\aVac]`aYa_aEEEE########################
@HELIUM_000100422_612GNAAXX:7:1:8684:19227#0/1 {"ali_dir":"left","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":100,"pairing_fast_score":0.691,"pairing_mismatches":{"(A:02)->(T:34)":101,"(C:02)->(A:11)":61,"(C:02)->(G:34)":92,"(C:18)->(T:34)":51,"(C:33)->(T:02)":33,"(G:02)->(C:34)":104,"(T:33)->(G:02)":39},"score":870,"score_norm":0.92,"seq_a_single":8,"seq_ab_match":92,"seq_b_single":8}
ccagcatgcacccattgagtctctgcacctatccatttttctcgtcgctttcttaactgaactttcccttgttttcgtaaagcaggatttggctcaggattgcccgtgcatgctgg
+
BBB@@A?<<CKCDCD>CDDDDDDDDDDDDDDDKDCDDDK8=KSC]d]dcdDYYUWLHNJN5@9EE?>BBDEEEEDDEEEEEEDEEEEEEEELEEEEEEEELEELEEEECACCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8630:10162#0/1 {"ali_dir":"right","ali_length":74,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":71,"pairing_fast_overlap":74,"pairing_fast_score":1,"score":5363,"score_norm":1,"seq_a_single":34,"seq_ab_match":74,"seq_b_single":34}
tacactctttccctacacgacgctcttccgatctccagagtagcgccattgagtctctgcacctatcttttggctcaggtttggctcaggattgccccgctactctggagatcggaagagcggttcagcaggaatgccgaga
+
;;?:979601-+0=AB?AC>@=CCCCCCC:.@C;ce_edXeeeeeeeeeeeeeeeeeecebeeeeeeeeeeeeeQecdeceeeeced`eeecefceebeaefaeccccCAA=<A'?3??==?<=A83A##############
@HELIUM_000100422_612GNAAXX:7:1:8773:17648#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gtgacgt:gtgacgt)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gtgacgt","obimultiplex_forward_tag":"gtgacgt","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gtgacgt","obimultiplex_reverse_tag":"gtgacgt","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":53,"pairing_fast_overlap":62,"pairing_fast_score":0.898,"pairing_mismatches":{"(c:32)->(t:02)":40},"score":1630,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
C=C???A?CCCCBCCAC+?<H@CEEEEEEEEEE@@CEE9J:?C]__\\KPYZ]ee`\cb`_`ZRMbcc_EEEEECBEBBDECCCCCCC;CCCCC>CCCCC
@HELIUM_000100422_612GNAAXX:7:1:8997:8829#0/1_sub[73..81] {"ali_dir":"right","ali_length":63,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (tgtccga:tgtccga)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tgtccga","obimultiplex_forward_tag":"tgtccga","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tgtccga","obimultiplex_reverse_tag":"tgtccga","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":60,"pairing_fast_overlap":63,"pairing_fast_score":1,"score":4539,"score_norm":1,"seq_a_single":45,"seq_ab_match":63,"seq_b_single":45}
ggccgcgtg
+
aeceeaede
@HELIUM_000100422_612GNAAXX:7:1:8670:12778#0/1 {"ali_dir":"right","ali_length":94,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":94,"pairing_fast_score":0.912,"pairing_mismatches":{"(A:34)->(G:02)":29,"(C:34)->(G:02)":23},"score":2749,"score_norm":0.979,"seq_a_single":14,"seq_ab_match":92,"seq_b_single":14}
cgctcctacgagctggatgacgccaaggaacacgaacggagaggagcacccctcctcacgagacaaccaatatcgacgactctcggcaacggatatctgtgcgactggagatcgcaagagcg
+
##############EEEEBEDELEEDEDLEEEDEEEEDEEEEEcaacQMV[LJaKO^^]aeacdacdVbddbXRcebZcb^SdbaRcaEEBEEEEEEEEEEEEFEEEE##############
@HELIUM_000100422_612GNAAXX:7:1:8631:16904#0/1 {"ali_dir":"left","ali_length":105,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":91,"pairing_fast_overlap":105,"pairing_fast_score":0.892,"pairing_mismatches":{"(A:02)->(C:34)":106,"(T:02)->(G:34)":87},"score":3905,"score_norm":0.971,"seq_a_single":3,"seq_ab_match":102,"seq_b_single":3}
tcactgcagtgccattgagtctctgcacctatccttttttattttcgctttctgaacctttgtttgttttcggaaaacgtgatttggctcaggattgccccactgcagtgg
+
CCCEEEEEEEEEECEEEEEbcaU\[aUVYZXaTZ_db`_eedadcdc_eeefcceeeddeedcd_deed[[d]acccEEEEEEEEELEEEEECEEEEEEELEEEELEECCC
@HELIUM_000100422_612GNAAXX:7:1:8644:2733#0/1 {"ali_dir":"right","ali_length":100,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":93,"pairing_fast_overlap":100,"pairing_fast_score":0.959,"pairing_mismatches":{"(T:02)->(G:34)":97},"score":5706,"score_norm":0.99,"seq_a_single":8,"seq_ab_match":99,"seq_b_single":8}
tccgatctccacgtagtcggggcaatcctgagccaaatcctgttttccgaaaacaaacaaaagttcagaaaaaaggataggtgcagagactcaatggcgactacgtggagatcgaa
+
C>C18C@CV`_\^]`]Z`^^a^Yecede`ecccdeeeceeeeeeee`edadeeddcdbee[^eae`deeaeed\dbec`^RW^XXZZZaabaEEEELCEEEEEEEEEE########
@HELIUM_000100422_612GNAAXX:7:1:8702:7175#0/1 {"ali_dir":"right","ali_length":70,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":67,"pairing_fast_overlap":70,"pairing_fast_score":1,"score":4920,"score_norm":1,"seq_a_single":38,"seq_ab_match":70,"seq_b_single":38}
agtctacactctttccctacacgacgctcttccgatctcccagcgtagagatatccgttgccgagagtcacagtggggtgttccttgacgccttcggtctacgctgggagatcggaggagcggttcagcaggaatgccgagaccga
+
###A@A=@:8943=6>?>?CCACBCBCCC?CBCCBCC?eedeedceaeeeefedeeeeeeeeeeeed]ceeceaebee_eeeeee^_eceebeab]ZaZ_XU\a]^a]AC####################################
@HELIUM_000100422_612GNAAXX:7:1:8674:10684#0/1 {"ali_dir":"left","ali_length":102,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":90,"pairing_fast_overlap":102,"pairing_fast_score":0.909,"pairing_mismatches":{"(A:02)->(C:34)":98,"(A:34)->(G:02)":43,"(G:33)->(T:02)":42},"score":2339,"score_norm":0.971,"seq_a_single":6,"seq_ab_match":99,"seq_b_single":6}
ccactcgagtcgggcaatcctgagccaaatccgtgttttgagaaaacaagggggttctcgaactagaatacaaaggaaaaggataggtgcagagactcaatgggactcgagtgg
+
CCCCCCCEEEBEEEEEEEDEEEEEEEEEEEEECEEEEEBAEKLEEEEE@Ba\a]b\Y[X^^_bCd`eeebe\ebeU`]aR_Vac_cEEEEEEEEEEELEEEEEAEEEECCCBCC
@HELIUM_000100422_612GNAAXX:7:1:9321:1523#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (atctctc:atctctc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"atctctc","obimultiplex_forward_tag":"atctctc","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"atctctc","obimultiplex_reverse_tag":"atctctc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":2461,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CBCCCCCCCCCCCCCCCACEIELEEEEDEEEECBB=EEBEKMZ_[a^JZa\[`eeMaaa_`TRe\db_]bacec\_\c[aECCCCCCCCCCCCCBCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8739:7178#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (agcagag:acgtacc)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"agcagag","obimultiplex_forward_tag":"agcagag","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"acgtacc","obimultiplex_reverse_tag":"acgtacc","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":55,"pairing_fast_overlap":62,"pairing_fast_score":0.932,"pairing_mismatches":{},"score":1905,"score_norm":0.984,"seq_a_single":46,"seq_ab_match":61,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaatagcttaaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCBBCCCCCCCCCCe]dcdbddbdd`dd_Q___\Q_Yaa`a]BBACBEEEEEAEEEDEEEEEEEEEEEEEELEEEECCCCCCCCCCCCCACCCCC
@HELIUM_000100422_612GNAAXX:7:1:8652:2336#0/1 {"ali_dir":"left","ali_length":89,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":82,"pairing_fast_overlap":89,"pairing_fast_score":0.953,"pairing_mismatches":{"(G:34)->(A:25)":29},"score":3518,"score_norm":0.989,"seq_a_single":19,"seq_ab_match":88,"seq_b_single":19}
cctctgaggattcggttggggcgaccaaggaataaatcatccttacaccataagacccattgatctttataaagaccctaataatagacctaaagataagctaccttagggataacagcctcagagg
+
CCCCCCCCCCCCCCCCCCC_c[]\WYQcCe_e\\^]La^ceccceeeeebccee`cba^YV_KTccdadd`ccdFEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEEECCCCCCCCCCCCCCCCCCC
@HELIUM_000100422_612GNAAXX:7:1:8804:11763#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"reverse","obimultiplex_error":"Cannot associate sample to the tag pair (gcgcgaa:gcgcgaa)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"gcgcgaa","obimultiplex_forward_tag":"gcgcgaa","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"gcgcgaa","obimultiplex_reverse_tag":"gcgcgaa","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":56,"pairing_fast_overlap":62,"pairing_fast_score":0.949,"pairing_mismatches":{"(c:15)->(t:34)":81},"score":1496,"score_norm":0.968,"seq_a_single":46,"seq_ab_match":60,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtactaccggcaacagcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCDCCCCCCCCCCC=CCCELEEEEEDEDDDDD=;@C?5;>;;BECE;BEEEEa]Y[XNXXVOOI]Z]XLYc_Z]]YTcZDCCCCCCCC@@CBC>=CCCC
@HELIUM_000100422_612GNAAXX:7:1:8666:9384#0/1 {"ali_dir":"right","ali_length":86,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":83,"pairing_fast_overlap":86,"pairing_fast_score":1,"score":5690,"score_norm":1,"seq_a_single":22,"seq_ab_match":86,"seq_b_single":22}
ctacacgacgctcttccgatctccgatctacggtcgccccaaccgaatttaatttttacttatttagtaggttaaattaagctctatagggtcttcttgcggttctggagatcggaagagcggttcagca
+
########A=C@C=AA<3CCCCeeUccccadc[eeZeaaa_a[_deeeeeeeeeeee_eefeeeedd_a`acdcdbdcc`cNc_`a_`\aa][YZZZ]\Z[IZYYYYT?#####################
@HELIUM_000100422_612GNAAXX:7:1:8637:4312#0/1 {"ali_dir":"right","ali_length":85,"mode":"alignment","obimultiplex_error":"No barcode identified","pairing_fast_count":74,"pairing_fast_overlap":85,"pairing_fast_score":0.902,"pairing_mismatches":{"(A:02)->(C:34)":99,"(C:02)->(G:34)":94},"score":4608,"score_norm":0.976,"seq_a_single":23,"seq_ab_match":83,"seq_b_single":23}
cctacacgacgctcttccgatctccttaggatcaagaagaccctatagagctttatctaaccatatgatatcatattcctagattcggttggggcgaccatcctaaggagatcgaaagagcgtttcagcag
+
###############A3B=6?=?X`U_cdeca\Yaa^ce`Vb_eeddeedeceeabebeeeeaeddeebcd_dadbRdY^Y^\XZYXVc_`aCLEEEELEBDEEEEEE#######################
@HELIUM_000100422_612GNAAXX:7:1:8876:21106#0/1_sub[28..127] {"ali_dir":"left","ali_length":62,"mode":"alignment","obimultiplex_amplicon_rank":"1/1","obimultiplex_direction":"forward","obimultiplex_error":"Cannot associate sample to the tag pair (tatctca:tatctca)","obimultiplex_forward_error":0,"obimultiplex_forward_match":"ttagataccccactatgc","obimultiplex_forward_matching":"strict","obimultiplex_forward_primer":"ttagataccccactatgc","obimultiplex_forward_proposed_tag":"tatctca","obimultiplex_forward_tag":"tatctca","obimultiplex_forward_tag_dist":0,"obimultiplex_reverse_error":0,"obimultiplex_reverse_match":"tagaacaggctcctctag","obimultiplex_reverse_matching":"strict","obimultiplex_reverse_primer":"tagaacaggctcctctag","obimultiplex_reverse_proposed_tag":"tatctca","obimultiplex_reverse_tag":"tatctca","obimultiplex_reverse_tag_dist":0,"pairing_fast_count":37,"pairing_fast_overlap":62,"pairing_fast_score":0.627,"pairing_mismatches":{"(A:02)->(C:09)":49,"(A:02)->(G:22)":61,"(C:02)->(G:29)":56,"(T:25)->(G:13)":36,"(T:30)->(G:28)":38},"score":1442,"score_norm":0.903,"seq_a_single":46,"seq_ab_match":56,"seq_b_single":46}
ttagccctaaacacaaataattataaaaacaaaattattcgccagagtcctaccggcaacggcccgaaactcaaaggacttggcggtgctttataccctt
+
CCCCCCCCCCCCCCCC@CBe^Xd_bddYYX^S[VS<P?X[\`ac_cE;3;<2;94G;9/<@<EEEEACCE?EEEBELBEDECCDCCCCDCCCBB:AACB@