# Sibling annex — architecture (discussion) Status: architecture decided (2026-08-14). Implementation not yet mandated. ## Two index spaces, uncorrelated Every kmer stored in a `Layer` lives in two independent index spaces: - **Iteration order**: its position when enumerating `unitigs.bin` (the superkmer file), deterministic but arbitrary with respect to slot. - **MPHF slot**: `MphfLayer::index(kmer)`, the number the MPHF assigns. The two are not correlated by any formula. Converting from one to the other requires either recomputing the MPHF (kmer → slot) or scanning the iteration stream (kmer → order). There is no `slot → kmer` operation: the MPHF is a one-way function, not an invertible bijection with a stored inverse. Any method that reconstructs a kmer from a bare slot number is wrong by construction, regardless of the mechanism used (MPHF re-hash, or evidence decode + direct unitig read). See `MphfLayer::kmer_at` (`obilayeredmap/src/mphf_layer.rs`) — flagged for removal, currently called from `obikphylo/siblings/build.rs` and `family_scan.rs` (since removed — see "Pending work" status below). ## Two pipelines, never mixed | | origin of the kmer | membership known? | correct mapping | |---|---|---|---| | **query pipeline** | external (caller-supplied) | no | `query`/`find`/`find_strict` — MPHF + evidence check | | **iteration pipeline** | enumerated from this layer's own `unitigs.bin` | yes, by construction | `index`/`index_batch` — MPHF only, no evidence | Evidence exists solely to answer "is this external kmer a member of the layer" for the query pipeline. Using it (or the MPHF) to go the other way — recover a kmer from a slot, or re-verify a kmer that was just produced by iterating the layer — is a conceptual error: evidence can be probabilistic (`Approx` mode), so any slot→kmer attempt is unsound in general, and pointless even in `Exact`/`Hybrid` mode since the kmer was already known. ## Sibling annex: an iteration-pipeline artifact only The sibling annex (`FamilyMask`/`SiblingAnnex`, `.psib`, `obicompactvec/src/siblingannex.rs`) records, per kmer, whether it is a family minorant and which family members are present in the index. Its only consumers (`obikphylo/siblings/stats.rs`, `family_scan.rs`) enumerate it exhaustively (`0..annex.len()`); no query-pipeline code path touches it. **Decision**: the annex must be persisted in iteration order, not slot order. This lets readers zip-iterate `Layer::iter_kmers()` and the annex file directly — one linear, cache-friendly pass, no MPHF/slot indirection, no `kmer_at`. It also enables specialized iterators building on this zip: minorants-only iteration, batch-of-kmers → batch-of-family-members, etc. Today the annex is built and stored in **slot** order (`build_layer_sibling_annex`, `siblings/build.rs`): `slot_kmer` is populated via `(0..n_slots).map(|slot| mphf.kmer_at(slot))`, and the origin `slot` is threaded through the whole cross-partition reconciliation pipeline (variant generation, `query_partition_with`, final `mask[slot].fetch_or(...)`). This must change to iterating `iter_kmers()`/`enumerate_kmers()` and threading the **iteration index** instead of the slot end to end — eliminating `kmer_at` from the build path entirely, not just the read path. No slot-indexed intermediate is needed even during construction; the iteration-order id is sufficient throughout. The cross-partition side of the same pipeline is unaffected: checking whether a generated family-variant kmer exists in another partition is a genuine query-pipeline operation (the variant's membership in the *target* partition is unknown) and must keep going through `KmerPartition::query_partition_with` (MPHF + evidence), never a raw `index()`. ## Pending work — done The plan above shipped: `obikphylo` (a new crate — phylo-domain extension traits over `obikindex::KmerIndex`/`obilayeredmap::Layer`, replacing the old `obikindex::siblings` module) builds and reads the annex purely in iteration order (`SiblingLayerExt::iter_siblings`/`iter_minorants`, both with batch variants, mirroring `Layer`'s own `KmerIter`/`KmerBatchIter` shape). `MphfLayer::kmer_at` has no remaining callers. A separate, unrelated bug surfaced during this work and was fixed (2026-08-14): `MphfLayer::enumerate_kmers_batch` computed its `batch_start_index` via the stdlib `.enumerate()` adapter, which counts *batches* (0, 1, 2…), not the cumulative k-mer offset the annex is actually keyed on — every batch past the first wrote its mask/annex entries at the wrong iteration-order position. Fixed by tracking a running offset instead; regression tests added (`sibling_annex_no_empty_masks_after_build`, `sibling_histogram_does_not_panic_on_partial_last_batch`). ## Performance: `build_sibling_annex` parallelism (2026-08-14) Investigated on a real multi-genome run (`phyloskims_sal_vac`, k=31/m=11). Baseline: mostly one active core, with short multi-core bursts — average ~3 cores. **Fixes that helped, kept:** - `CanonicalKmerOf::minimizer()` (`obikseq/src/kmer.rs`) — a direct O(k) bit-arithmetic minimiser for a single isolated k-mer, replacing a `RollingStat` instance fed byte-by-byte through an ASCII round-trip (used by `helpers::partition_of`, called for every generated family variant). ~3x wall-clock improvement on its own, confirmed by sampling (`obiskbuilder::rolling_stat`/`obikentropy` frames disappeared from the hot path). `CanonicalKmerOf::partition()` added alongside it (wraps `minimizer().seq_hash() & mask`, the same routing rule `KmerPartition`/`RoutableSuperKmer` use). - Cross-partition resolution (`outgoing.par_iter()` in `build_layer_sibling_annex`) parallelised at the *partition* level — one Rayon task per non-empty `outgoing[dest]` bucket. For k=31/m=11, a central-base substitution changes the winning minimiser (and thus the destination partition) only when that window overlaps the central base: ~11 of the 21 possible windows do, so ~10/21 (≈48%) of generated variants route right back to the partition already being built. That self bucket ends up far larger than any other, so the per-partition split pinned one thread to it alone while the rest of the pool finished instantly — confirmed by sampling: one thread solid in `MphfLayer::find`, everyone else idle. Fixed by splitting each non-empty bucket into `total_queries / n_workers` (capped 4096) chunks *before* `par_iter()`, preserving per-partition mmap locality (each chunk stays contiguous within one partition) while letting Rayon spread an oversized bucket across several threads. Net effect of both fixes together: ~3 cores average → ~10-13 cores average on the same run, and a projected total build time of ~1h15 down to ~30min on the real `phyloskims_sal_vac` run this was measured against. - `TracedBar`'s ETA (`obisys/src/progress.rs`) was silently starved: the custom progress message and the self-computed ETA text used to share one `pb.set_message()` slot, with the ETA holding off for 2s after any custom message — fine when custom messages are rare, broken once `build_sibling_annex`'s per-partition callback fires more often than that. Fixed by keeping the two texts in separate fields, composed together on every render instead of one overwriting the other. **Tried and reverted — do not repeat blindly:** - Parallelising the *outer* partition loop in `build_sibling_annex` with `obikindex::PartitionRunner` (already used by `merge`/`build_layers`), splitting a fixed core budget between outer (partition) and inner (pipeline + resolution) concurrency so their product wouldn't exceed the budget. Measured *worse*: throughput dropped over time (26 partitions/5min → 38/11-12min) and peak resolution concurrency fell from ~11-12 cores to ~7-8. Cause: this capped the resolution burst — which scales very well on its own — to make room for outer concurrency, and running several partitions' resolution at once scatters access across multiple partitions' mmap regions at once, working against the locality `outgoing`'s per-partition grouping exists for. `PartitionRunner` stayed exported from `obikindex` (`new_capped` too) since it's general-purpose, but nothing in `obikphylo` calls it. - Splitting resolution chunks even finer (`/(n_workers*8)`, cap 1024, instead of `/n_workers`, cap 4096) to smooth the residual sawtooth. Measured ~10% *slower*, wider dips, not narrower. Reverted to the original chunk sizing. **Known remaining limitation, not yet worth fixing:** within one layer, the four stages (sequential `unitigs.bin` read → parallel generation → parallel resolution → sequential annex write) never overlap — confirmed by 1s-interval sampling: generation alone occupies ~17 threads evenly, but the next layer's read/generation never starts until the current layer's resolution and write are both done. This produces a real, periodic (~layer duration) alternation between "many cores" and "few cores" that neither of the fixes above touches, since both operate *within* one layer's resolution step. The only remaining lever is overlapping consecutive layers (e.g. a depth-2 pipeline: start layer N+1's read/generation while layer N's resolution/write is still running) — a real restructuring, not a parameter tweak, and explicitly *not* to be combined with the reverted budget-capping idea above (let each phase use however many cores it naturally wants; only the *scheduling* needs to overlap). Deferred, not started. ## Cross-partition batch resolution — current state vs. the batched-accumulator design (discussion, 2026-08-14) `family_scan.rs::scan_layer_families` (shared by `snp_pseudo_alignment`, `sibling_annex_stats`, `cardinality_tally`, `scan_family_pairs`) already implements most of a dispatch/accumulate/resolve pipeline: generation (cheap, CPU-only — builds `outgoing[dest_partition]` from `FamilyMask` and buckets cross-partition queries) runs on an `obipipeline::throttle` + `make_pipe!` stage, decoupled from resolution (I/O-bound, `rayon::par_iter` *across partitions*, one generated batch resolved at a time, never several concurrently — this ordering is deliberate, see the module's own docs on a reverted concurrent-batch-resolution attempt that scattered mmap access). The fast/slow mode gate (`PartitionCache::fast_mode`, `cache.rs:162-163`) already exists: `n_layers <= 7` (checked once from the first non-empty partition's `PartitionMeta::n_layers`, documented as identical across every partition of an index — a structural, build-time property, never a per-partition state) decides whether `FamilyMask`'s recorded `layer_value` can be trusted to skip straight to the right layer (`find_presence_batch_fast`) or must fall back to scanning every layer of the destination partition (`find_presence_batch`). **Real gap, confirmed not implemented**: resolution is triggered by the *source* batch finishing (`FAMILY_BATCH = 65536` minorants read from the scanned layer), not by an *output* accumulator filling up. Since most central-base variants of a family route back to the same partition being scanned (~48% per the k=31/m=11 measurement above), a `FAMILY_BATCH`'s `outgoing[dest]` is large for the local/self partition and thin for the other ~255 (or however many) destination partitions — each of those gets resolved at low query density every batch instead of being accumulated across several source batches until resolving it is worthwhile. This is distinct from, and not fixed by, the fast/slow layer gate above. Redesign sketched (not built): per-destination accumulators decoupled from `FAMILY_BATCH`, flushed on reaching a size threshold instead of on source-batch completion — a "hot" accumulator for the partition being scanned (sharded one-per-generation-worker, no lock, since all `n_workers` pipeline workers write to it concurrently — this differs from an earlier, simpler mental model of "one thread owns one layer's local collector," which doesn't hold here since `n_workers` threads cooperate on scanning *one* layer at a time, not one thread per layer) and "cold" mutex-per-partition accumulators for the rest, low contention expected since traffic to any single cold destination is a small fraction of total. This breaks the current strict-iteration-order delivery of `on_family` (today: a reorder buffer keyed by batch, since a whole `FAMILY_BATCH` resolves atomically). With cross-batch accumulation, a family only becomes complete once *every* accumulator holding one of its outgoing queries has flushed, at unpredictable, independent times — no longer streamable strictly in order without a large, unbounded pending buffer. Resolution sketched: replace order-dependent consumers with coordinate-addressed writes instead of order-dependent appends (see `PseudoAlignment` idea below) wherever possible, since `sibling_annex_stats`'s reduction (plain counts) is already order-independent and needs nothing here. **Superseded 2026-08-15** by the `--subsample`/`--shannon` design below, which sidesteps the accumulator redesign for now: bounding the number of families actually resolved per layer (via sampling) keeps per-layer resolution volume small enough that the batch-density problem above stops mattering in practice for these two consumers. The accumulator redesign remains relevant for a future *unsampled, full-index* run, but is not required to ship `--subsample`/`--shannon`. ## Pseudo-alignment at scale — pruning is unavoidable (discussion, 2026-08-14/15) The reference run (`phyloskims_sal_vac`-scale bacterial test set, `iqtree.fasta`) produced a dense alignment for 13 genomes × 383,965 sites (4.8 MB) — trivially small. The in-progress plant index is expected to carry on the order of 9 billion minorant families; a dense byte-per-cell alignment at that column count is unbuildable regardless of genome count (hundreds of GB even at a handful of genomes). Long-term ambition is 6,000– 8,000 genomes on a large machine, which makes the per-cell cost dominant in the other dimension too. Pruning the retained family set before materializing anything is mandatory, not an optimization. **Already free**: `family_size() < 2` (no sibling variant registered at all) is a zero-cost structural filter, read directly off `FamilyMask` bits, already applied in `snp_pseudo_alignment`. Insufficient alone — per the ~80-85% mono-family estimate from earlier discussion, this only brings 9 billion down to roughly 1.3-1.8 billion, still unusable. **Entropy definition — settled 2026-08-15, correcting an earlier wrong turn.** The project does **not** encode families as IUPAC ambiguity codes interpreted the classical way (Fitch-parsimony subset-compatibility, or ML's "one true state, uncertain which"); see `docmd/theory/evolutionary_distances.md` ("Why the IUPAC/DNA encoding used for the first `--snp` test was wrong") and the Sankoff resolution that followed it. The real model is a genuine 16-state alphabet (the powerset of `{A,C,G,T}`, `∅` included as a real state) scored with a *calibrated pairwise cost matrix* (`obikphylo::cardcomp::pairwise_cost_matrix`, `cmd/phylo/sankoff.rs`), not a compatibility/subset relation between states. Under that model, each of the 16 states — including multi-bit ones like `AC` — is a first-class, independently-costed state, not an uncertainty encoding of a single true base. So: **entropy over the 15 non-empty states (`∅` excluded, matching the earlier decision to exclude genomes where the family is absent) is the correct informativeness measure** for this project — not a 4-symbol reduction, which would discard exactly the cardinality/composition information the calibrated cost matrix is built to exploit. ## `--subsample` / `--shannon` — sampling strategy (decided 2026-08-15) Goal: make both the pseudo-alignment (`--snp`) and a Shannon-entropy diagnostic usable at any index scale, from the 13-genome bacterial reference run up to the 9-billion-family plant index, without requiring the batched-accumulator redesign above. **`--subsample N`** (integer, families to retain): bounds the pseudo-alignment to `N` minorant families, sampled **proportionally per layer** among non-monomorphic minorants (`family_size >= 2`) — this sidesteps the need for a true global reservoir merge across layers while still approximating a uniform sample over the whole index, and directly answers the earlier open question of global-vs-per-layer selection scope. Three passes, in order: 1. **Global count** (cheap, structural, parallel across layers — same shape as the existing `sibling_family_size_histogram`, extended to report a **per-layer** breakdown rather than one index-wide aggregate): for each layer, `count_layer` = number of non-monomorphic minorants. Gives `total_count = Σ count_layer`. 2. **Per-layer proportional reservoir sampling** (cheap, structural, one pass per layer, no cross-partition resolution): `N_layer = round(N × count_layer / total_count)`. Since `N_layer` is a proportion of `count_layer`, it can never exceed it as long as `N <= total_count` — the one edge case is `total_count <= N`, in which case sampling is skipped entirely and *every* non-monomorphic minorant of every layer is kept (no reservoir needed, `N` was never a real constraint). Otherwise: Algorithm-R reservoir sampling over the layer's non-monomorphic minorant indices, producing `N_layer` iteration-order indices directly, no intermediate full list ever materialized. 3. **Filtered resolution** (the expensive step, the existing `scan_layer_families` engine, unchanged): re-scan the layer, generating and resolving cross-partition queries **only** for the indices selected in step 2 (cheap membership test against a small per-layer index set) — this is what keeps `--subsample` cheap even on an unsampled-scale index, since the cross-partition resolution volume is bounded by `N`, not by the layer's true size. Steps 2 and 3 cannot be merged into one pass: true single-pass reservoir sampling would waste step-3's expensive resolution work on candidates later evicted by the reservoir. Step 1 must fully complete (every layer) before step 2 can start for any layer, since `total_count` is a global quantity. **`--shannon`** (no argument): emits a CSV of per-family Shannon entropy (15 non-empty states, `∅`/absent genomes excluded from the denominator, per the settled definition above). Independent of `--subsample` — entropy is computed and written per family as soon as its `genome_mask` resolves, O(1) memory per family, so it streams fine even unsampled at full index scale (a time cost, not a memory one). Combined with `--subsample N`, it delivers the original exploratory diagnostic (e.g. `--subsample 1000000 --shannon`) directly from this general machinery, rather than a purpose-built one-off script. Validated end-to-end (2026-08-15) against real data: `--sibling-hist` on `phyloskims_sal_vac` (91 real genomes, k=31/m=11, 256 partitions × 2 layers) confirms the ~9-billion-family estimate almost exactly (8,925,068,238 total, 97.9% monomorphic — a sharper mono fraction than the ~80-85% earlier guess, corrected here). `--subsample`/`--shannon` on the smaller 20-genome bacterial reference (`benchmark/global_index_presence`) produced a sample size within rounding of the request (99,742/100,000) and a [0.8,1.2)-bucket share (40.7%) matching the full unsampled population (41.6%) — the two histograms only diverged wildly (5‰ vs 41.6%) under a real bug in `reservoir_sample_layer` (see next section), now fixed. **Bug found and fixed (2026-08-15): `family_idx` numbering mismatch.** `scan_layer_families`'s `family_idx` counts *every minorant* of a layer (monomorphic ones included, since `iter_minorants_batch` filters only on `is_minorant()`), not the raw annex slot (`SiblingAnnex::get(slot)` spans every k-mer, minorant or not) and not a counter over non-monomorphic minorants alone. `subsample.rs`'s `reservoir_sample_layer` originally stored raw slot numbers in its `HashSet` selection, which drifts away from `family_idx` as soon as *any* monomorphic minorant is seen — i.e. almost immediately, since ~98% of minorants are monomorphic. Fixed by tracking two separate counters: `family_idx` (every minorant, matching `scan_layer_families`) and `seen` (non-monomorphic minorants only, what Algorithm R actually samples over) — only `family_idx` values are ever stored in the selection set. The existing unit test never exercised this (its fixture has exactly one non-monomorphic family, always hitting the "keep everything" shortcut) — a stronger fixture with several interleaved monomorphic/non-monomorphic families would be needed to catch a regression here automatically; not yet written. ## Cheap entropy pre-filtering — row-marginal sums (idea, not implemented, 2026-08-15) Motivation: on real data (bacterial reference, full unsampled run), only ~5‰ of non-monomorphic minorants fall in the `[0.5, 1.5]` bit band judged phylogenetically interesting (entropy too low = uninformative near-invariant site; too high = saturated/noisy, see `family_entropy`'s 15-state discussion) — roughly 1 in 10,000 minorants overall. Computing exact entropy for every candidate just to discard 99.99% of them is wasteful at the full 9-billion scale. **The idea**: `PersistentBitMatrix::col_view(c)` gives a genome's whole presence column as a `BitSliceView` (sequential, no MPHF, no cross-partition routing — purely local to one layer's own matrix). Accumulating `TempCompactIntVecBuilder::inc_present(col)` (already exists in `obicompactvec/src/builder.rs:121`, along with `add`/`min`/`max`/`diff` on `IntSliceView` — no new low-level API needed) over every column of a layer produces `coverage[slot]`: how many genomes carry each exact k-mer, in one sequential per-layer pass, entirely decoupled from family/sibling structure. Persisted once per layer, a family's members' coverage could then be looked up via a plain local MPHF `index()` (cheap) instead of a full cross-partition presence resolution (`find_presence_batch`) — the expensive part today is specifically the cross-partition/cross-layer routing to a sibling's own matrix, not the bit-reading itself, and `coverage[slot]` sidesteps that routing entirely by moving the cost into a one-time, purely local, embarrassingly-parallel build step. **Why not implemented**: `coverage[slot]` is a per-member marginal — summing members' coverages to approximate a family's entropy silently assumes no genome carries more than one member at once. It cannot represent or detect joint co-occurrence (a genome carrying both `A` and `C` at once, i.e. a combined 15-symbol state) at all, which is exactly the phenomenon `family_entropy`'s 15-state definition exists to capture (see `CardinalityTally`/`cardinality_transition_probs`, the project's own existing machinery for this same co-occurrence structure, built for the Sankoff matrix calibration). A family that is in truth uniformly `AC` across every carrying genome would look like a well-balanced 2-state split under the marginal approximation (entropy ≈ 1) while its true 15-state entropy is 0 — i.e. the marginal proxy's failure mode lands on exactly the "saturated, uninformative" tail this pre-filter would need to catch, undermining the point. It stays plausible as a coarse filter for the *low* tail only (a dominant single member's marginal share reliably predicts low true entropy too), but not as a stand-in for the high tail — not pursued further for now. ## Two entropy definitions kept side by side, for comparison (2026-08-15) `--shannon`'s CSV carries both `entropy15` (`family_entropy` — the settled 15-non-empty-state definition, see above) and `entropy4` (`family_entropy_4` — plain nucleotide reduction), computed from the same already-resolved `genome_mask`, not from the marginal approximation above. A genome carrying several bases at once contributes to *each* base's count (counted once per base present, not fractionally split, not folded into one combined state) — a genome polymorphic for the family is present at more than one base by construction, so it is expected to count more than once; the denominator is the total base-occurrence count, not the genome count (the two coincide only when no genome carries more than one base). Kept side by side specifically to measure, on real data, how much the two diverge — not yet analyzed. ## `PersistentSparseBitMatrix` — implemented and measured (2026-08-15) A row-major (k-mer-major), deduplicated sparse alternative to `obicompactvec::PersistentBitMatrix`, motivated by the same sparsity that drove `--subsample`/`--shannon` above, but pursued as a foundational storage-layer change rather than an index-level workaround. Full design history, rationale, and rejected alternatives (external Elias-Fano crates, `cacheline-ef`, a single unsplit `dict_id` array) are in the dedicated implementation plan (`vivid-mapping-tiger.md` at the time of writing — the content below is the durable summary, not a pointer to a session-scoped file). Also directly informed by Alanko, Bille, Gørtz, Navarro, Puglisi, "Compact Data Structures for Collections of Sets" (2025, `biblio/Alanko et al. - Compact Data Structures for Collections of Sets.pdf`) — this design implements only their exact-duplicate special case (a plain dedup dictionary), not their full subset-containment hierarchy. **Design**: four on-disk components, each mmap-backed, built once per layer (matching how the rest of the build pipeline already works — never the whole multi-billion-row index at once): an `is_multi` rank-capable flag per row (singleton vs. multi-genome), a fixed-bit-width array for singleton rows (genome index directly, `ceil(log2(n_cols))` bits), a separate fixed-bit-width array for multi-genome rows (`dict_id`, `ceil(log2(n_distinct_multi_sets))` bits — kept apart from the singleton array specifically because `n_distinct_multi_sets` can be large in absolute terms even when multi-genome rows are a small *fraction* of all rows, and a single shared array would force every row, singletons included, to pay the wider width), and a deduplicated dictionary of distinct multi-genome sets (Elias-Fano-encoded byte offsets + a varint-encoded values blob). New low-level primitives added to `obicompactvec` to build this: `PersistentFixedIntVec` (arbitrary, runtime-parameterized bit width, width 0 included — needed once a real bug surfaced, see below), `PersistentRankSelectBitVec` (rank1/rank0/select1 on top of the crate's existing `count_ones`, using `common_traits::SelectInWord`), `EliasFano` (composes the two). A new `BinaryMatrix` trait (`n`, `n_cols`, `row`/`fill_row`, `fill_sub_matrix`, `count_ones`) unifies dense and sparse at the one call site that needs both interchangeably (`obikphylo::siblings::cache::Mat`) — column-oriented methods (`col`, `col_view`, the `partial_*_dist_matrix` family) stay dense-only. **Two real bugs caught by tests, not by inspection**: (1) `EliasFano::open` re-derived its low-bits width from the persisted low-vector file's own width byte; the zero-width case was built with a dummy 1-bit placeholder (the builder rejected true width 0), so every reopened value silently doubled. Fixed by making `PersistentFixedIntVec` genuinely support width 0 (no storage, `get` always 0) instead of working around the limitation in `EliasFano`. (2) An empty row (cardinality 0 — not expected on a real built index, but not guarded against either) was recorded as a singleton at genome 0, indistinguishable on read-back from a *real* singleton at genome 0. Fixed by routing cardinality-0 rows through the dictionary path (a genuine empty entry) instead of the singleton shortcut. Both caught by `obicompactvec`'s test suite (142 tests, including disk-reopen round-trips that drop every builder/mmap before reopening fresh), not by manual review — worth remembering next time a "this edge case can't happen in practice" shortcut is tempting. **Measured on real data** (`layer_1` of `phyloskims_sal_vac`'s `part_00018`, 30,246,774 rows, 91 genomes — `#[ignore]`d benchmarks in `obikphylo/src/siblings/tests.rs`): | | dense | sparse | ratio | |---|---|---|---| | on-disk size | 328.1MB | 43.7MB | **7.5x** smaller | | build time / peak RSS | — | 4.26s / 628MB | (per-layer, in-memory construction — comfortable) | | row access, sequential (2M reads) | 43ns/row | 32ns/row | sparse **faster** (smaller structure, better cache fit) | | row access, random (2M reads) | 409ns/row | 85ns/row | sparse **~4.8x faster** (the real `--shannon`/family-lookup shape) | | column access, one full column (30.2M rows) | 11.5ms | 993ms | sparse **86x slower** (no native column method — every read decodes a full row to keep one bit) | The row-access wins (both directions) weren't the design's stated goal — compactness was — but turn out real: dense's genome-major layout scatters a single row read across a much bigger file, which costs more than sparse's rank/select/varint decode once the file is this much smaller. The column-access cost is the flip side of the same layout choice, and is exactly what the next item below exists to fix. **Next, not yet planned**: rewrite `partial_jaccard_dist_matrix`/ `partial_hamming_dist_matrix`/etc. (`obicompactvec/src/bitmatrix/pairwise.rs`) as a row-major co-occurrence accumulation (`O(Σ_rows k²)`, per-row increments into an `NxN` genome-pair counter — the known alternative to today's column-fold, plausibly cheaper on data this sparse, not just a fallback) so `obikindex`'s `--metric`/distance-matrix path can use the sparse type without the measured 86x column-access penalty. Needs its own design pass (in particular how it plugs into the `BitPartials`/ `ColumnWeights` traits so both matrix types keep serving `--metric`) before implementation — not just "port the loop", a genuinely different algorithm. Also still deferred, unchanged from the implementation plan: full Alanko et al. subset-hierarchy compression (only the exact-duplicate special case is built), a sparse `PersistentCompactIntMatrix` (count matrices), and BRWT-style column-correlation exploitation. ## Wired into `pack` and the sibling-annex build path (2026-08-15) `PersistentSparseBitMatrix` went from a validated but unused type to a real, selectable on-disk format: - **Generic `Layer`**: `obilayeredmap::Layer`'s presence-only methods (`n_cols`, `sub_matrix`, `fill_sub_matrix`) are generic over any `D: LayerData> + BinaryMatrix`, not hardcoded to `PersistentBitMatrix` — `PersistentSparseBitMatrix` implements `LayerData` (`open`/`read`) the same way. `find_slot`/`index_batch` were already generic over any `D: LayerData`, so they needed no change. Verified by `obilayeredmap`'s `presence_layer_generic_over_sparse_matches_dense` test: build a dense presence layer, convert it to sparse via `build_from_dense`, open both as `Layer`/`Layer` on the same directory, assert `n_cols`/`sub_matrix`/`find_slot` agree. (This test must stay at `k=4` with mutually non-colliding canonical 4-mers across its input sequences — `K`/`M` are process-wide `AtomicUsize`s in test builds, not thread-local, so a test using a different `k` races every other test in the same crate binary; a k=11 version of this test passed alone but failed under the full `obilayeredmap` suite for exactly that reason before being fixed.) - **`obikphylo::siblings::cache::Mat`** gained a third variant, `SparsePresence(Layer)`, alongside `Count` and `Presence` — every method (`find_slot`, `index_batch`, `iter_minorants_batch`, `n_cols`, `fill_sub_matrix_carries`) dispatches to it identically to `Presence`, since both go through the same generic `Layer` code. `PartitionCache::build` picks the variant per layer by checking for `presence/is_multi.prsb` (the sparse format's own marker file, see the design section above) before falling back to the dense open path. - **`pack_sparse_bit_matrix`** (new, `obicompactvec::bitmatrix::sparse`): `pack --sparse`'s entry point. Idempotent (checks `is_multi.prsb` first); packs to dense `matrix.pbmx` first if that hasn't happened yet (the dense→sparse transpose needs random row access, which only the packed/columnar dense forms give), then `build_from_dense`s the sparse form into the same directory and deletes `matrix.pbmx` — old-format files are removed only after the new format is fully written, mirroring `pack_bit_matrix`'s own crash-safety convention. - **CLI**: `obikmer pack --sparse` threads a `sparse: bool` through `KmerIndex::pack_matrices` (all other call sites — `select`, `merge`, `finalize_indexed` — pass `false`, unchanged dense behaviour). Count matrices are untouched by `--sparse` (no sparse `PersistentCompactIntMatrix` — see "still deferred" above). - **End-to-end coverage**: `obikphylo::siblings::tests:: sibling_annex_works_after_pack_sparse` builds a two-genome index, packs it `--sparse`, asserts `is_multi.prsb` exists, then runs `build_sibling_annex` and checks the resulting `FamilyMask`s match the dense-path test (`sibling_annex_one_sibling_each`) exactly — proves the sparse format round-trips through the real build pipeline (`PartitionCache` sparse-detection included), not just the `obicompactvec`/`obilayeredmap` unit layers below it. Full workspace `cargo test` (all crates, unit + doc tests) green after this change.