# Kmer — implementation ## Memory layout `Kmer` is a `#[repr(transparent)]` newtype over `u64`: ```rust #[repr(transparent)] pub struct Kmer(u64); ``` Nucleotides are packed 2 bits each, **left-aligned**, MSB-first. Nucleotide 0 occupies bits 63–62; nucleotide i occupies bits 63−2i and 62−2i. The low 64−2k bits are always zero. k is **not stored** — it is a parameter of every operation that needs it, and will be owned by the future collection-level indexer. | 63–62 | 61–60 | … | 63−2(k−1)−1 to 63−2(k−1) | 63−2k down to 0 | |-------|-------|---|--------------------------|-----------------| | nt 0 | nt 1 | … | nt k−1 | zero padding | ## Encoding `Kmer::from_ascii(ascii, k)` encodes the first k bytes of an ASCII slice using the shared `ENC` table (see [SuperKmer — ASCII encoding](superkmer.md#ascii-encoding-and-decoding)): ```rust for i in 0..k { val = (val << 2) | encode_base(ascii[i]) as u64; } Kmer(val << (64 - 2 * k)) ``` Zero allocation — result lives on the stack. ## Decoding `write_ascii(k, buf)` appends k ASCII characters to a caller-supplied `Vec` using the shared `DEC4` table: one lookup per 4 nucleotides, two partial-byte lookups for the remainder. No allocation in the hot path. `to_ascii(k)` is a convenience wrapper that allocates and returns a `Vec`; intended for tests and display only. ## Reverse complement Computed as pure arithmetic — no lookup table, no memory access: ```rust let x = !self.0; // complement let x = x.swap_bytes(); // reverse bytes let x = ((x >> 4) & 0x0F0F0F0F0F0F0F0F) | ((x & 0x0F0F0F0F0F0F0F0F) << 4); // swap nibbles let x = ((x >> 2) & 0x3333333333333333) | ((x & 0x3333333333333333) << 2); // swap 2-bit groups Kmer(x << (64 - 2 * k)) ``` After complementing, bytes are reversed (`swap_bytes`), then nibbles, then 2-bit groups — restoring 2-bit nucleotides to their correct positions in reverse order. A final left-shift realigns to MSB. Zero allocation — result lives on the stack. ## Canonical form ```rust pub fn canonical(&self, k: usize) -> Self { let rc = self.revcomp(k); if self.0 <= rc.0 { *self } else { rc } } ``` Lexicographic minimum of forward and reverse-complement, comparing the raw `u64` values directly (left-aligned encoding makes this equivalent to nucleotide-wise comparison). Zero allocation — result lives on the stack.