first batch

Former-commit-id: 1eecb206a17c4aff21d1170b48db134ce3c4f14e
This commit is contained in:
Eric Coissac
2025-03-01 16:15:28 +01:00
parent 4e51d42b85
commit 2c012eec8e
596 changed files with 5247 additions and 77743 deletions
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@@ -33,7 +33,9 @@ include $(CFGDIR)targets/help.targ
all:: install
extract:
extract: $(PKGDIR)
$(PKGDIR): $(PKGTAR)
test -d $(PKGDIR) || mkdir $(PKGDIR)
test -d $(PKGDIR)/bin || $(TAR) zxf $(PKGTAR) -C $(PKGDIR) --strip-components 1
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0e7930365b9633d9ade6060599cb0f31825e04d8
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f5be2a5e3aae2d9a175aa804f12db0db035cfda5
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The BLAST+ release notes can be found in
http://www.ncbi.nlm.nih.gov/books/NBK131777
@@ -0,0 +1,19 @@
PUBLIC DOMAIN NOTICE
National Center for Biotechnology Information
This software/database is a "United States Government Work" under the
terms of the United States Copyright Act. It was written as part of
the author's official duties as a United States Government employee and
thus cannot be copyrighted. This software/database is freely available
to the public for use. The National Library of Medicine and the U.S.
Government have not placed any restriction on its use or reproduction.
Although all reasonable efforts have been taken to ensure the accuracy
and reliability of the software and data, the NLM and the U.S.
Government do not and cannot warrant the performance or results that
may be obtained by using this software or data. The NLM and the U.S.
Government disclaim all warranties, express or implied, including
warranties of performance, merchantability or fitness for any particular
purpose.
Please cite the author in any work or product based on this material.
@@ -0,0 +1,10 @@
BLAST Command Line Applications
The NCBI Basic Local Alignment Search Tool (BLAST) finds regions of
local similarity between sequences. The program compares nucleotide or
protein sequences to sequence databases and calculates the statistical
significance of matches. BLAST can be used to infer functional and
evolutionary relationships between sequences as well as help identify
members of gene families.
For more information, visit http://blast.ncbi.nlm.nih.gov
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#!/usr/bin/perl
# $Id: update_blastdb.pl 446090 2014-09-11 12:12:27Z ivanov $
# ===========================================================================
#
# PUBLIC DOMAIN NOTICE
# National Center for Biotechnology Information
#
# This software/database is a "United States Government Work" under the
# terms of the United States Copyright Act. It was written as part of
# the author's official duties as a United States Government employee and
# thus cannot be copyrighted. This software/database is freely available
# to the public for use. The National Library of Medicine and the U.S.
# Government have not placed any restriction on its use or reproduction.
#
# Although all reasonable efforts have been taken to ensure the accuracy
# and reliability of the software and data, the NLM and the U.S.
# Government do not and cannot warrant the performance or results that
# may be obtained by using this software or data. The NLM and the U.S.
# Government disclaim all warranties, express or implied, including
# warranties of performance, merchantability or fitness for any particular
# purpose.
#
# Please cite the author in any work or product based on this material.
#
# ===========================================================================
#
# Author: Christiam Camacho
#
# File Description:
# Script to download the pre-formatted BLAST databases from the NCBI ftp
# server.
#
# ===========================================================================
use strict;
use warnings;
use Net::FTP;
use Getopt::Long;
use Pod::Usage;
use File::stat;
use Digest::MD5;
use Archive::Tar;
use List::MoreUtils qw(uniq);
use constant NCBI_FTP => "ftp.ncbi.nlm.nih.gov";
use constant BLAST_DB_DIR => "/blast/db";
use constant USER => "anonymous";
use constant PASSWORD => "anonymous";
use constant DEBUG => 0;
use constant MAX_DOWNLOAD_ATTEMPTS => 3;
use constant EXIT_FAILURE => 2;
# Process command line options
my $opt_verbose = 1;
my $opt_quiet = 0;
my $opt_force_download = 0;
my $opt_help = 0;
my $opt_passive = 0;
my $opt_timeout = 120;
my $opt_showall = 0;
my $opt_show_version = 0;
my $opt_decompress = 0;
my $result = GetOptions("verbose+" => \$opt_verbose,
"quiet" => \$opt_quiet,
"force" => \$opt_force_download,
"passive" => \$opt_passive,
"timeout=i" => \$opt_timeout,
"showall" => \$opt_showall,
"version" => \$opt_show_version,
"decompress" => \$opt_decompress,
"help" => \$opt_help);
$opt_verbose = 0 if $opt_quiet;
die "Failed to parse command line options\n" unless $result;
pod2usage({-exitval => 0, -verbose => 2}) if $opt_help;
pod2usage({-exitval => 0, -verbose => 2}) unless (scalar @ARGV or
$opt_showall or
$opt_show_version);
my $exit_code = 0;
$|++;
# Connect and download files
my $ftp = &connect_to_ftp() unless ($opt_show_version);
if ($opt_show_version) {
my $revision = '$Revision: 446090 $';
$revision =~ s/\$Revision: | \$//g;
print "$0 version $revision\n";
} elsif ($opt_showall) {
print "$_\n" foreach (sort(&get_available_databases()));
} else {
my @files = sort(&get_files_to_download());
my @files2decompress;
$exit_code = &download(\@files, \@files2decompress);
if ($exit_code == 1) {
foreach (@files2decompress) {
$exit_code = &decompress($_);
last if ($exit_code != 1);
}
}
}
$ftp->quit() unless ($opt_show_version);
exit($exit_code);
# Connects to NCBI ftp server
sub connect_to_ftp
{
my %ftp_opts;
$ftp_opts{'Passive'} = 1 if $opt_passive;
$ftp_opts{'Timeout'} = $opt_timeout if ($opt_timeout >= 0);
$ftp_opts{'Debug'} = 1 if ($opt_verbose > 1);
my $ftp = Net::FTP->new(NCBI_FTP, %ftp_opts)
or die "Failed to connect to " . NCBI_FTP . ": $!\n";
$ftp->login(USER, PASSWORD)
or die "Failed to login to " . NCBI_FTP . ": $!\n";
$ftp->cwd(BLAST_DB_DIR);
$ftp->binary();
print "Connected to NCBI\n" if $opt_verbose;
return $ftp;
}
# Gets the list of available databases on NCBI FTP site
sub get_available_databases
{
my @blast_db_files = $ftp->ls();
my @retval = ();
foreach (@blast_db_files) {
next unless (/\.tar\.gz$/);
push @retval, &extract_db_name($_);
}
return uniq @retval;
}
# Obtains the list of files to download
sub get_files_to_download
{
my @blast_db_files = $ftp->ls();
my @retval = ();
if ($opt_verbose > 2) {
print "Found the following files on ftp site:\n";
print "$_\n" for (@blast_db_files);
}
if (grep(/gss/, @ARGV) and not grep(/gss_annot/, @ARGV)) {
push @ARGV, qw(gss_annot);
}
for my $requested_db (@ARGV) {
for my $file (@blast_db_files) {
next unless ($file =~ /\.tar\.gz$/);
if ($file =~ /^$requested_db\..*/) {
push @retval, $file;
}
}
}
if ($opt_verbose) {
for my $requested_db (@ARGV) {
unless (grep(/$requested_db/, @retval)) {
print STDERR "$requested_db not found, skipping.\n"
}
}
}
return @retval;
}
# Download the requested files only if their checksum files are missing or if
# these (or the archives) are newer in the FTP site. Returns 0 if no files were
# downloaded, 1 if at least one file was downloaded (so that this can be the
# application's exit code)
sub download($$)
{
my @requested_dbs = @ARGV;
my @files2download = @{$_[0]};
my $files2decompress = $_[1];
my $retval = 0;
for my $file (@files2download) {
my $attempts = 0; # Download attempts for this file
if ($opt_verbose and &is_multivolume_db($file) and $file =~ /\.00\./) {
my $db_name = &extract_db_name($file);
my $nvol = &get_num_volumes($db_name, @files2download);
print "Downloading $db_name (" . $nvol . " volumes) ...\n" unless ($opt_quiet);
}
# We preserve the checksum files as evidence of the downloaded archive
my $checksum_file = "$file.md5";
my $new_download = (-e $checksum_file ? 0 : 1);
my $update_available = ($new_download or
((stat($checksum_file))->mtime < $ftp->mdtm($checksum_file)));
if (-e $file and (stat($file)->mtime < $ftp->mdtm($file))) {
$update_available = 1;
}
download_file:
if ($opt_force_download or $new_download or $update_available) {
print "Downloading $file..." if $opt_verbose;
$ftp->get($file);
unless ($ftp->get($checksum_file)) {
print STDERR "Failed to download $checksum_file!\n";
return EXIT_FAILURE;
}
my $rmt_digest = &read_md5_file($checksum_file);
my $lcl_digest = &compute_md5_checksum($file);
print "\nRMT $file Digest $rmt_digest" if (DEBUG);
print "\nLCL $file Digest $lcl_digest\n" if (DEBUG);
if ($lcl_digest ne $rmt_digest) {
unlink $file, $checksum_file;
if (++$attempts >= MAX_DOWNLOAD_ATTEMPTS) {
print STDERR "too many failures, aborting download!\n";
return EXIT_FAILURE;
} else {
print "corrupt download, trying again.\n";
goto download_file;
}
}
push @$files2decompress, $file if ($opt_decompress);
print " [OK]\n" if $opt_verbose;
$retval = 1 if ($retval == 0);
} else {
if ($opt_decompress and -f $file) {
push @$files2decompress, $file;
$retval = 1;
} else {
my $msg = ($opt_decompress
? "The contents of $file are up to date in your system."
: "$file is up to date.");
print "$msg\n" if $opt_verbose;
}
}
}
return $retval;
}
# Try to decompress using /bin/tar as Archive::Tar is known to be slower (as
# it's pure perl)
sub _decompress_impl($)
{
my $file = shift;
unless ($^O =~ /win/i) {
local $ENV{PATH} = "/bin:/usr/bin";
my $cmd = "gzip -cd $file 2>/dev/null | tar xf - 2>/dev/null";
return 1 unless (system($cmd));
}
return Archive::Tar->extract_archive($file, 1);
}
# Decompresses the file passed as its argument
# Returns 1 on success, and 2 on failure, printing an error to STDERR
sub decompress($)
{
my $file = shift;
print "Decompressing $file ..." unless ($opt_quiet);
my $succeeded = &_decompress_impl($file);
unless ($succeeded) {
my $msg = "Failed to decompress $file ($Archive::Tar::error), ";
$msg .= "please do so manually.";
print STDERR "$msg\n";
return EXIT_FAILURE;
}
unlink $file; # Clean up archive, but preserve the checksum file
print " [OK]\n" unless ($opt_quiet);
return 1;
}
sub compute_md5_checksum($)
{
my $file = shift;
my $digest = "N/A";
if (open(DOWNLOADED_FILE, $file)) {
binmode(DOWNLOADED_FILE);
$digest = Digest::MD5->new->addfile(*DOWNLOADED_FILE)->hexdigest;
close(DOWNLOADED_FILE);
}
return $digest;
}
sub read_md5_file($)
{
my $md5file = shift;
open(IN, $md5file);
$_ = <IN>;
close(IN);
my @retval = split;
return $retval[0];
}
# Determine if a given pre-formatted BLAST database file is part of a
# multi-volume database
sub is_multivolume_db
{
my $file = shift;
return 1 if ($file =~ /\.\d{2,3}\.tar\.gz$/);
return 0;
}
# Extracts the database name from the pre-formatted BLAST database archive file
# name
sub extract_db_name
{
my $file = shift;
my $retval = "";
if (&is_multivolume_db($file)) {
$retval = $1 if ($file =~ m/(.*)\.\d{2,3}\.tar\.gz$/);
} else {
$retval = $1 if ($file =~ m/(.*)\.tar\.gz$/);
}
return $retval;
}
# Returns the number of volumes for a BLAST database given the file name of a
# pre-formatted BLAST database and the list of all databases to download
sub get_num_volumes
{
my $db = shift;
my $retval = 0;
foreach (@_) {
if (/$db/) {
if (/.*\.(\d{2,3})\.tar\.gz$/) {
$retval = int($1) if (int($1) > $retval);
}
}
}
return $retval + 1;
}
__END__
=head1 NAME
B<update_blastdb.pl> - Download pre-formatted BLAST databases from NCBI
=head1 SYNOPSIS
update_blastdb.pl [options] blastdb ...
=head1 OPTIONS
=over 2
=item B<--decompress>
Downloads, decompresses the archives in the current working directory, and
deletes the downloaded archive to save disk space, while preserving the
archive checksum files (default: false).
B<Note>: Using this option may require more computing resources than using
your system's native decompression tool(s).
=item B<--showall>
Show all available pre-formatted BLAST databases (default: false). The output
of this option lists the database names which should be used when
requesting downloads or updates using this script.
=item B<--passive>
Use passive FTP, useful when behind a firewall (default: false).
=item B<--timeout>
Timeout on connection to NCBI (default: 120 seconds).
=item B<--force>
Force download even if there is a archive already on local directory (default:
false).
=item B<--verbose>
Increment verbosity level (default: 1). Repeat this option multiple times to
increase the verbosity level (maximum 2).
=item B<--quiet>
Produce no output (default: false). Overrides the B<--verbose> option.
=item B<--version>
Prints this script's version. Overrides all other options.
=back
=head1 DESCRIPTION
This script will download the pre-formatted BLAST databases requested in the
command line from the NCBI ftp site.
=head1 EXIT CODES
This script returns 0 on successful operations that result in no downloads, 1
on successful operations that downloaded files, and 2 on errors.
=head1 BUGS
Please report them to <blast-help@ncbi.nlm.nih.gov>
=head1 COPYRIGHT
See PUBLIC DOMAIN NOTICE included at the top of this script.
=cut
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@@ -0,0 +1,2 @@
The user manual is available in http://www.ncbi.nlm.nih.gov/books/NBK279690
Release notes are available in http://www.ncbi.nlm.nih.gov/books/NBK131777
@@ -0,0 +1,186 @@
PACKAGE_NAME: blast
PACKAGE_VERSION: 2.2.31
PACKAGE_SIGNATURE: ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
PACKAGE_CONFIG: Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastp
blastp: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastn
blastn: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastx
blastx: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> tblastn
tblastn: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> tblastx
tblastx: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> psiblast
psiblast: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> rpsblast
rpsblast: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> rpstblastn
rpstblastn: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> makembindex
makembindex: 0.0.0
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> segmasker
segmasker: 1.0.0
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> dustmasker
dustmasker: 1.0.0
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> windowmasker
windowmasker: 1.0.0
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> deltablast
deltablast: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> makeblastdb
makeblastdb: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastdbcmd
blastdbcmd: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastdb_aliastool
blastdb_aliastool: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> convert2blastmask
convert2blastmask: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastdbcheck
blastdbcheck: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> makeprofiledb
makeprofiledb: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blast_formatter
blast_formatter: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:20:04
ICC_1313-ReleaseMT64--x86_64-unknown-linux2.6.32-gnu2.12-coremake11
Linux64-Centos : icc : ./c++/compilers/unix/ICC.sh --with-strip --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-build-root-sfx=--Linux64-Centos-icc --with-ncbi-public --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
@@ -0,0 +1,2 @@
The BLAST+ release notes can be found in
http://www.ncbi.nlm.nih.gov/books/NBK131777
@@ -0,0 +1,19 @@
PUBLIC DOMAIN NOTICE
National Center for Biotechnology Information
This software/database is a "United States Government Work" under the
terms of the United States Copyright Act. It was written as part of
the author's official duties as a United States Government employee and
thus cannot be copyrighted. This software/database is freely available
to the public for use. The National Library of Medicine and the U.S.
Government have not placed any restriction on its use or reproduction.
Although all reasonable efforts have been taken to ensure the accuracy
and reliability of the software and data, the NLM and the U.S.
Government do not and cannot warrant the performance or results that
may be obtained by using this software or data. The NLM and the U.S.
Government disclaim all warranties, express or implied, including
warranties of performance, merchantability or fitness for any particular
purpose.
Please cite the author in any work or product based on this material.
@@ -0,0 +1,10 @@
BLAST Command Line Applications
The NCBI Basic Local Alignment Search Tool (BLAST) finds regions of
local similarity between sequences. The program compares nucleotide or
protein sequences to sequence databases and calculates the statistical
significance of matches. BLAST can be used to infer functional and
evolutionary relationships between sequences as well as help identify
members of gene families.
For more information, visit http://blast.ncbi.nlm.nih.gov
@@ -0,0 +1,8 @@
#
# these are non-universal, x84_64 architecture only, binaries
# from the original NCBI distribution
#
# binaries have been liposucted by:
# ditto --rsrc -arch x84_64
#
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@@ -0,0 +1,405 @@
#!/usr/bin/perl
# $Id: update_blastdb.pl 446090 2014-09-11 12:12:27Z ivanov $
# ===========================================================================
#
# PUBLIC DOMAIN NOTICE
# National Center for Biotechnology Information
#
# This software/database is a "United States Government Work" under the
# terms of the United States Copyright Act. It was written as part of
# the author's official duties as a United States Government employee and
# thus cannot be copyrighted. This software/database is freely available
# to the public for use. The National Library of Medicine and the U.S.
# Government have not placed any restriction on its use or reproduction.
#
# Although all reasonable efforts have been taken to ensure the accuracy
# and reliability of the software and data, the NLM and the U.S.
# Government do not and cannot warrant the performance or results that
# may be obtained by using this software or data. The NLM and the U.S.
# Government disclaim all warranties, express or implied, including
# warranties of performance, merchantability or fitness for any particular
# purpose.
#
# Please cite the author in any work or product based on this material.
#
# ===========================================================================
#
# Author: Christiam Camacho
#
# File Description:
# Script to download the pre-formatted BLAST databases from the NCBI ftp
# server.
#
# ===========================================================================
use strict;
use warnings;
use Net::FTP;
use Getopt::Long;
use Pod::Usage;
use File::stat;
use Digest::MD5;
use Archive::Tar;
use List::MoreUtils qw(uniq);
use constant NCBI_FTP => "ftp.ncbi.nlm.nih.gov";
use constant BLAST_DB_DIR => "/blast/db";
use constant USER => "anonymous";
use constant PASSWORD => "anonymous";
use constant DEBUG => 0;
use constant MAX_DOWNLOAD_ATTEMPTS => 3;
use constant EXIT_FAILURE => 2;
# Process command line options
my $opt_verbose = 1;
my $opt_quiet = 0;
my $opt_force_download = 0;
my $opt_help = 0;
my $opt_passive = 0;
my $opt_timeout = 120;
my $opt_showall = 0;
my $opt_show_version = 0;
my $opt_decompress = 0;
my $result = GetOptions("verbose+" => \$opt_verbose,
"quiet" => \$opt_quiet,
"force" => \$opt_force_download,
"passive" => \$opt_passive,
"timeout=i" => \$opt_timeout,
"showall" => \$opt_showall,
"version" => \$opt_show_version,
"decompress" => \$opt_decompress,
"help" => \$opt_help);
$opt_verbose = 0 if $opt_quiet;
die "Failed to parse command line options\n" unless $result;
pod2usage({-exitval => 0, -verbose => 2}) if $opt_help;
pod2usage({-exitval => 0, -verbose => 2}) unless (scalar @ARGV or
$opt_showall or
$opt_show_version);
my $exit_code = 0;
$|++;
# Connect and download files
my $ftp = &connect_to_ftp() unless ($opt_show_version);
if ($opt_show_version) {
my $revision = '$Revision: 446090 $';
$revision =~ s/\$Revision: | \$//g;
print "$0 version $revision\n";
} elsif ($opt_showall) {
print "$_\n" foreach (sort(&get_available_databases()));
} else {
my @files = sort(&get_files_to_download());
my @files2decompress;
$exit_code = &download(\@files, \@files2decompress);
if ($exit_code == 1) {
foreach (@files2decompress) {
$exit_code = &decompress($_);
last if ($exit_code != 1);
}
}
}
$ftp->quit() unless ($opt_show_version);
exit($exit_code);
# Connects to NCBI ftp server
sub connect_to_ftp
{
my %ftp_opts;
$ftp_opts{'Passive'} = 1 if $opt_passive;
$ftp_opts{'Timeout'} = $opt_timeout if ($opt_timeout >= 0);
$ftp_opts{'Debug'} = 1 if ($opt_verbose > 1);
my $ftp = Net::FTP->new(NCBI_FTP, %ftp_opts)
or die "Failed to connect to " . NCBI_FTP . ": $!\n";
$ftp->login(USER, PASSWORD)
or die "Failed to login to " . NCBI_FTP . ": $!\n";
$ftp->cwd(BLAST_DB_DIR);
$ftp->binary();
print "Connected to NCBI\n" if $opt_verbose;
return $ftp;
}
# Gets the list of available databases on NCBI FTP site
sub get_available_databases
{
my @blast_db_files = $ftp->ls();
my @retval = ();
foreach (@blast_db_files) {
next unless (/\.tar\.gz$/);
push @retval, &extract_db_name($_);
}
return uniq @retval;
}
# Obtains the list of files to download
sub get_files_to_download
{
my @blast_db_files = $ftp->ls();
my @retval = ();
if ($opt_verbose > 2) {
print "Found the following files on ftp site:\n";
print "$_\n" for (@blast_db_files);
}
if (grep(/gss/, @ARGV) and not grep(/gss_annot/, @ARGV)) {
push @ARGV, qw(gss_annot);
}
for my $requested_db (@ARGV) {
for my $file (@blast_db_files) {
next unless ($file =~ /\.tar\.gz$/);
if ($file =~ /^$requested_db\..*/) {
push @retval, $file;
}
}
}
if ($opt_verbose) {
for my $requested_db (@ARGV) {
unless (grep(/$requested_db/, @retval)) {
print STDERR "$requested_db not found, skipping.\n"
}
}
}
return @retval;
}
# Download the requested files only if their checksum files are missing or if
# these (or the archives) are newer in the FTP site. Returns 0 if no files were
# downloaded, 1 if at least one file was downloaded (so that this can be the
# application's exit code)
sub download($$)
{
my @requested_dbs = @ARGV;
my @files2download = @{$_[0]};
my $files2decompress = $_[1];
my $retval = 0;
for my $file (@files2download) {
my $attempts = 0; # Download attempts for this file
if ($opt_verbose and &is_multivolume_db($file) and $file =~ /\.00\./) {
my $db_name = &extract_db_name($file);
my $nvol = &get_num_volumes($db_name, @files2download);
print "Downloading $db_name (" . $nvol . " volumes) ...\n" unless ($opt_quiet);
}
# We preserve the checksum files as evidence of the downloaded archive
my $checksum_file = "$file.md5";
my $new_download = (-e $checksum_file ? 0 : 1);
my $update_available = ($new_download or
((stat($checksum_file))->mtime < $ftp->mdtm($checksum_file)));
if (-e $file and (stat($file)->mtime < $ftp->mdtm($file))) {
$update_available = 1;
}
download_file:
if ($opt_force_download or $new_download or $update_available) {
print "Downloading $file..." if $opt_verbose;
$ftp->get($file);
unless ($ftp->get($checksum_file)) {
print STDERR "Failed to download $checksum_file!\n";
return EXIT_FAILURE;
}
my $rmt_digest = &read_md5_file($checksum_file);
my $lcl_digest = &compute_md5_checksum($file);
print "\nRMT $file Digest $rmt_digest" if (DEBUG);
print "\nLCL $file Digest $lcl_digest\n" if (DEBUG);
if ($lcl_digest ne $rmt_digest) {
unlink $file, $checksum_file;
if (++$attempts >= MAX_DOWNLOAD_ATTEMPTS) {
print STDERR "too many failures, aborting download!\n";
return EXIT_FAILURE;
} else {
print "corrupt download, trying again.\n";
goto download_file;
}
}
push @$files2decompress, $file if ($opt_decompress);
print " [OK]\n" if $opt_verbose;
$retval = 1 if ($retval == 0);
} else {
if ($opt_decompress and -f $file) {
push @$files2decompress, $file;
$retval = 1;
} else {
my $msg = ($opt_decompress
? "The contents of $file are up to date in your system."
: "$file is up to date.");
print "$msg\n" if $opt_verbose;
}
}
}
return $retval;
}
# Try to decompress using /bin/tar as Archive::Tar is known to be slower (as
# it's pure perl)
sub _decompress_impl($)
{
my $file = shift;
unless ($^O =~ /win/i) {
local $ENV{PATH} = "/bin:/usr/bin";
my $cmd = "gzip -cd $file 2>/dev/null | tar xf - 2>/dev/null";
return 1 unless (system($cmd));
}
return Archive::Tar->extract_archive($file, 1);
}
# Decompresses the file passed as its argument
# Returns 1 on success, and 2 on failure, printing an error to STDERR
sub decompress($)
{
my $file = shift;
print "Decompressing $file ..." unless ($opt_quiet);
my $succeeded = &_decompress_impl($file);
unless ($succeeded) {
my $msg = "Failed to decompress $file ($Archive::Tar::error), ";
$msg .= "please do so manually.";
print STDERR "$msg\n";
return EXIT_FAILURE;
}
unlink $file; # Clean up archive, but preserve the checksum file
print " [OK]\n" unless ($opt_quiet);
return 1;
}
sub compute_md5_checksum($)
{
my $file = shift;
my $digest = "N/A";
if (open(DOWNLOADED_FILE, $file)) {
binmode(DOWNLOADED_FILE);
$digest = Digest::MD5->new->addfile(*DOWNLOADED_FILE)->hexdigest;
close(DOWNLOADED_FILE);
}
return $digest;
}
sub read_md5_file($)
{
my $md5file = shift;
open(IN, $md5file);
$_ = <IN>;
close(IN);
my @retval = split;
return $retval[0];
}
# Determine if a given pre-formatted BLAST database file is part of a
# multi-volume database
sub is_multivolume_db
{
my $file = shift;
return 1 if ($file =~ /\.\d{2,3}\.tar\.gz$/);
return 0;
}
# Extracts the database name from the pre-formatted BLAST database archive file
# name
sub extract_db_name
{
my $file = shift;
my $retval = "";
if (&is_multivolume_db($file)) {
$retval = $1 if ($file =~ m/(.*)\.\d{2,3}\.tar\.gz$/);
} else {
$retval = $1 if ($file =~ m/(.*)\.tar\.gz$/);
}
return $retval;
}
# Returns the number of volumes for a BLAST database given the file name of a
# pre-formatted BLAST database and the list of all databases to download
sub get_num_volumes
{
my $db = shift;
my $retval = 0;
foreach (@_) {
if (/$db/) {
if (/.*\.(\d{2,3})\.tar\.gz$/) {
$retval = int($1) if (int($1) > $retval);
}
}
}
return $retval + 1;
}
__END__
=head1 NAME
B<update_blastdb.pl> - Download pre-formatted BLAST databases from NCBI
=head1 SYNOPSIS
update_blastdb.pl [options] blastdb ...
=head1 OPTIONS
=over 2
=item B<--decompress>
Downloads, decompresses the archives in the current working directory, and
deletes the downloaded archive to save disk space, while preserving the
archive checksum files (default: false).
B<Note>: Using this option may require more computing resources than using
your system's native decompression tool(s).
=item B<--showall>
Show all available pre-formatted BLAST databases (default: false). The output
of this option lists the database names which should be used when
requesting downloads or updates using this script.
=item B<--passive>
Use passive FTP, useful when behind a firewall (default: false).
=item B<--timeout>
Timeout on connection to NCBI (default: 120 seconds).
=item B<--force>
Force download even if there is a archive already on local directory (default:
false).
=item B<--verbose>
Increment verbosity level (default: 1). Repeat this option multiple times to
increase the verbosity level (maximum 2).
=item B<--quiet>
Produce no output (default: false). Overrides the B<--verbose> option.
=item B<--version>
Prints this script's version. Overrides all other options.
=back
=head1 DESCRIPTION
This script will download the pre-formatted BLAST databases requested in the
command line from the NCBI ftp site.
=head1 EXIT CODES
This script returns 0 on successful operations that result in no downloads, 1
on successful operations that downloaded files, and 2 on errors.
=head1 BUGS
Please report them to <blast-help@ncbi.nlm.nih.gov>
=head1 COPYRIGHT
See PUBLIC DOMAIN NOTICE included at the top of this script.
=cut
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@@ -0,0 +1,2 @@
The user manual is available in http://www.ncbi.nlm.nih.gov/books/NBK279690
Release notes are available in http://www.ncbi.nlm.nih.gov/books/NBK131777
@@ -0,0 +1,186 @@
PACKAGE_NAME: blast
PACKAGE_VERSION: 2.2.31
PACKAGE_SIGNATURE: LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
PACKAGE_CONFIG: IntelMAC : universal : ./c++/compilers/unix/GCC.sh 4.2 --without-debug --without-pcre --with-mt --with-openmp --with-flat-makefile --with-universal=i386,x86_64 --with-ncbi-public --with-build-root-sfx=--IntelMAC-universal --without-ccache --without-caution --without-makefile-auto-update --with-projects=scripts/projects/blast/project.lst --with-internal
>>>>> blastp
blastp: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> blastn
blastn: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> blastx
blastx: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> tblastn
tblastn: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> tblastx
tblastx: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> psiblast
psiblast: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> rpsblast
rpsblast: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> rpstblastn
rpstblastn: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> makembindex
makembindex: 0.0.0
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> segmasker
segmasker: 1.0.0
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> dustmasker
dustmasker: 1.0.0
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> windowmasker
windowmasker: 1.0.0
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> deltablast
deltablast: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> makeblastdb
makeblastdb: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> blastdbcmd
blastdbcmd: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> blastdb_aliastool
blastdb_aliastool: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> convert2blastmask
convert2blastmask: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> blastdbcheck
blastdbcheck: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> makeprofiledb
makeprofiledb: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
>>>>> blast_formatter
blast_formatter: 2.2.31+
Package: blast 2.2.31, build Jun 2 2015 10:47:46
LLVMGCC_421-ReleaseMT--x86_64-apple-darwin12.6.0-xcoremake2
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