163 lines
58 KiB
Plaintext
163 lines
58 KiB
Plaintext
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ò> I9ÄtRA;L$uë"f D f D H‰ÐH‹PH9Ðt1;Huï[A\ÉÃ[L‰àA\ÉÃH�£" H�5K# ¹" ¿ èEíÿÿëž;HtÏ[A\É1Àà € UH…öH‰åATI‰üSH‰ót%H;5‡D t H‹6H�=## è6óÿÿH‰ oD ‰U> Hƒ=aD tMD‹D> E…ÀxAI‹D$‹
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H�¬ H�5¦! H˜¹z H‰ÇH‰E˜èŠîÿÿH‹M¨H‹u˜H�–! I‰ÇH‰Ç1ÀèV L‰ÿèVðÿÿH‹U°L‰ÿH‰H‹M¨H�s! H‹u˜1Àè. H�u̺ L‰ÿèíÿÿ‹}ÌH�3 H�5ƒ H‰E¸¹ ÿÏHcÿH�<H�<ý èîÿÿI‰Å‹EÌA‰E D‹]ÌE…ÛBH‹E°L‰hD‹U¤E…Ò…� H‹E°HÇ@ H‹E°HƒÄH[A\A]A^A_Éà € € M�uE1äf „ IcÜH‹}¸AÿÄH� [HÁãI�4 Lëè¢÷ÿÿH‹CH�@I�ÆH‰CD9eÌÌH‹E°L‰hD‹U¤E…Ò„zÿÿÿ € H‹M¨H‹u˜H�j L‰ÿ1Àè H‹u°L‰ÿè» H‹U°H‰BH‹E°HƒÄH[A\A]A^A_ÉÃUH‰åL‰eèL‰mðA‰ÕL‰uøH‰]àI‰öHƒì ‰Îè‹ùÿÿE…íI‰Ä~SA‹61Û90t*f. „ L‰çèÈùÿÿ…ÀuÿÃA9Ýt-HcÃA‹4†A94$uàH‹ $L‹d$¸ L‹l$L‹t$ÉÃf D H‹ $L‹d$1ÀL‹l$L‹t$ÉÃ���������Uº H‰åAWAVAUATSHƒì8H‰u°H�uÌè ëÿÿ‹}ÌH�s H�5v ¹ H‰E¸ÿÏHcÿHÁçHƒÇ(è!ìÿÿI‰Ç‹EÌA‰‹EÌ…À!HƒÄ8L‰ø[A\A]A^A_Éà € „ I�GE1öH‰E H�EÈH‰ÆH‰E¨éÄ D ‹H�÷ H�5 ¹1 L�kA‰D$‹{ÿÇHcÿè¥ëÿÿI‰$HcSH‰ÇL‰îèž HcSI‹$H�5Ú ¹5 Æ ‹{H� ÿÇHcÿèfëÿÿI‰D$H‰ÇHcCHcSI�t èX HcSI‹D$Æ H‹E°H‹PHcC HƒÂH�@H�ÂI‰D$AÿÆD;uÌ�ÿÿÿH‹u¨L‹e H‹}¸IcÆHÁàIÄè:èÿÿH…ÀH‰ÃtÐ1ÀèÛçÿÿ…À„ÿÿÿ‹;èìçÿÿ‹{‰èâçÿÿ‹{‰Cè×çÿÿ‹{ ‰CèÌçÿÿ‰C éäþÿÿÿ%&9 ÿ%(9 ÿ%*9 ÿ%,9 ÿ%.9 ÿ%09 ÿ%29 ÿ%49 ÿ%69 ÿ%89 ÿ%:9 ÿ%<9 ÿ%>9 ÿ%@9 ÿ%B9 ÿ%D9 ÿ%F9 ÿ%H9 ÿ%J9 ÿ%L9 ÿ%N9 ÿ%P9 ÿ%R9 ÿ%T9 ÿ%V9 ÿ%X9 ÿ%Z9 ÿ%\9 ÿ%^9 ÿ%`9 ÿ%b9 ÿ%d9 ÿ%f9 ÿ%h9 ÿ%j9 ÿ%l9 ÿ%n9 ÿ%p9 L� i8 é@„ÿÿL� E9 é4„ÿÿL� Á8 é(„ÿÿL�
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9 é „ÿÿL� a8 é„ÿÿL� •8 é„ÿÿL� ©8 éøƒÿÿL� 8 éìƒÿÿL� ™8 éàƒÿÿL� µ8 éÔƒÿÿL� A8 éȃÿÿL� -8 鼃ÿÿL� 8 é°ƒÿÿL� }8 餃ÿÿL� ‘8 阃ÿÿL� u8 錃ÿÿL� �8 逃ÿÿL� u7 étƒÿÿL� �7 éhƒÿÿL� �7 é\ƒÿÿL� Ù7 éPƒÿÿL� Å7 éDƒÿÿL� I7 é8ƒÿÿL� u7 é,ƒÿÿL� Q7 é ƒÿÿL� ½7 éƒÿÿL� ©7 éƒÿÿL� 8 éü‚ÿÿL� ù7 éð‚ÿÿL� 8 éä‚ÿÿL� á6 éØ‚ÿÿL� -7 éÌ‚ÿÿL� ù6 éÀ‚ÿÿL� Õ6 é´‚ÿÿL� q7 騂ÿÿL� 7 霂ÿÿL� ¹7 é�‚ÿÿL� U7 é„‚ÿÿ%a %Y-%m-%d %H:%M:%S %Z #%d#, %s
|
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%d:%02d:%02d %d %d
|
|||
|
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%6d %s %s %c%c %d %4.3f %3.2f
|
|||
|
|
bG taxon taxa Total pair count : %d
|
|||
|
|
ecoprimer.c Cannot Allocate ordered pairs Total good pair count : %u
|
|||
|
|
# 0.2 # ecoPrimer version %s
|
|||
|
|
# Rank level optimisation : %s
|
|||
|
|
# max error count by oligonucleotide : %d
|
|||
|
|
# Restricted to %s:
|
|||
|
|
# %d : %s (%s)
|
|||
|
|
# Ignore %s:
|
|||
|
|
# strict primer quorum : %3.2f
|
|||
|
|
# example quorum : %3.2f
|
|||
|
|
# counterexample quorum : %3.2f
|
|||
|
|
# database : %s
|
|||
|
|
# Database is constituted of %5d examples corresponding to %5d %s
|
|||
|
|
# and %5d counterexamples corresponding to %5d %s
|
|||
|
|
# amplifiat length between [%d,%d] bp
|
|||
|
|
# amplifiat length larger than %d bp
|
|||
|
|
# amplifiat length smaller than %d bp
|
|||
|
|
# DB sequences are considered as circular # DB sequences are considered as linear
|
|||
|
|
Unknown taxon level: '%s'
|
|||
|
|
Error on prefix allocation ------------------------------------------
|
|||
|
|
ecoPrimer Version %s
|
|||
|
|
synopsis : finding primers and measureing the quality of primers and barcode region
|
|||
|
|
usage: ./ecoPrimer [options]
|
|||
|
|
options:
|
|||
|
|
-d : [D]atabase : to match the expected format, the database
|
|||
|
|
has to be formated first by the ecoPCRFormat.py program located.
|
|||
|
|
in the ecoPCR/tools directory.
|
|||
|
|
ecoPCRFormat.py creates three file types :
|
|||
|
|
.sdx : contains the sequences
|
|||
|
|
.tdx : contains information concerning the taxonomy
|
|||
|
|
.rdx : contains the taxonomy rank
|
|||
|
|
|
|||
|
|
ecoPrimer needs all the file type. As a result, you have to write the
|
|||
|
|
database radical without any extension. For example /ecoPrimerDB/fstvert
|
|||
|
|
|
|||
|
|
-e : [E]rror : max error allowed by oligonucleotide (0 by default)
|
|||
|
|
|
|||
|
|
-h : [H]elp - print <this> help
|
|||
|
|
|
|||
|
|
-i : [I]gnore the given taxonomy id.
|
|||
|
|
|
|||
|
|
-l : minimum [L]ength : define the minimum amplication length.
|
|||
|
|
|
|||
|
|
-L : maximum [L]ength : define the maximum amplicationlength.
|
|||
|
|
|
|||
|
|
-r : [R]estricts the search to the given taxonomic id.
|
|||
|
|
|
|||
|
|
-c : Consider that the database sequences are [c]ircular
|
|||
|
|
|
|||
|
|
-q : Strict matching [q]uorum, percentage of the sequences in which strict primers are found. By default it is 70
|
|||
|
|
|
|||
|
|
-s : [S]ensitivity quorum
|
|||
|
|
|
|||
|
|
-t : required [t]axon level for results, by default the results are computed at species level
|
|||
|
|
|
|||
|
|
-x : false positive quorum
|
|||
|
|
|
|||
|
|
-D : set in [d]ouble strand mode
|
|||
|
|
|
|||
|
|
-S : Set in [s]ingle strand mode
|
|||
|
|
|
|||
|
|
-U : No multi match
|
|||
|
|
|
|||
|
|
Table result description :
|
|||
|
|
column 1 : serial number
|
|||
|
|
column 2 : primer1
|
|||
|
|
column 3 : primer2
|
|||
|
|
column 4 : good/bad
|
|||
|
|
column 5 : in sequence count
|
|||
|
|
column 6 : out sequence count
|
|||
|
|
column 7 : yule
|
|||
|
|
column 8 : in taxa count
|
|||
|
|
column 9 : out taxa count
|
|||
|
|
column 10 : coverage
|
|||
|
|
column 11 : unambiguously identified taxa
|
|||
|
|
column 12 : specificity
|
|||
|
|
column 13 : minimum amplified length
|
|||
|
|
column 14 : maximum amplified length
|
|||
|
|
column 15 : average amplified length
|
|||
|
|
http://www.grenoble.prabi.fr/trac/ecoPrimer/
|