ngsfilter and ecopcr: now check for primers too long for apat library to
handle (31bp max) and switch to version 3.0.1b23
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@ -18,6 +18,7 @@
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#include "libecoPCR/ecoPCR.h"
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#include "libecoPCR/libthermo/nnparams.h"
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#include "libecoPCR/libapat/apat.h"
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#include "obi_ecopcr.h"
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#include "obidms.h"
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@ -365,8 +366,6 @@ static int print_seq(Obiview_p i_view, Obiview_p o_view,
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int32_t i;
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// TODO add check for primer longer than MAX_PAT_LEN (32)
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// Get sequence id
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seq_id = obi_get_str_with_elt_idx_and_col_p_in_view(i_view, i_id_column, i_idx, 0);
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@ -751,6 +750,20 @@ int obi_ecopcr(const char* i_dms_name,
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o1c = complementPattern(o1);
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o2c = complementPattern(o2);
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// check for primers equal or longer than MAX_PAT_LEN (32)
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if (strlen(primer1) >= MAX_PAT_LEN)
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{
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obi_set_errno(OBI_ECOPCR_ERROR);
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obidebug(1, "\nError: first primer is too long, needs to be < 32bp (%s)", primer1);
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return -1;
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}
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if (strlen(primer2) >= MAX_PAT_LEN)
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{
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obi_set_errno(OBI_ECOPCR_ERROR);
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obidebug(1, "\nError: second primer is too long, needs to be < 32bp (%s)", primer2);
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return -1;
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}
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// Open input DMS
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i_dms = obi_open_dms(i_dms_name, false);
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if (i_dms == NULL)
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