obitools/obitools3
2
0
Fork 0
Code Issues Pull Requests Actions Packages Projects Releases 66 Wiki Activity

multithreaded obiclean working but not cleaned

Browse Source
This commit is contained in:
Celine Mercier
2019-06-19 17:29:58 +02:00
parent fd0b7a9177
commit 9b4c3537f9
1 changed files with 168 additions and 137 deletions
Download Patch File Download Diff File Expand all files Collapse all files

133
src/obi_clean.c
View File

@ -160,33 +160,23 @@ int obi_clean(const char* dms_name,
{
char* o_view_name_temp = NULL;
float p;
index_t i, j, l;
index_t l;
index_t k;
index_t seq_count;
index_t* line_selection = NULL;
double score;
bool above_threshold;
int lcs_length;
int ali_length;
Kmer_table_p ktable;
Obi_blob_p blob1;
Obi_blob_p blob2;
int lcs_min;
int sample_count;
int sample;
int s1_count;
int s2_count;
bool head;
int head_count;
int internal_count;
int singleton_count;
int ind_sample_count;
char status;
int samp;
byte_t* alignment_result_array = NULL;
byte_t ali_result;
int* complete_sample_count_array = NULL;
int* sample_count_array = NULL;
Obi_blob_p* blob_array = NULL;
OBIDMS_p dms = NULL;
@ -201,9 +191,6 @@ int obi_clean(const char* dms_name,
OBIDMS_column_p singletoncount_column = NULL;
OBIDMS_column_p samplecount_column = NULL;
byte_t no;
byte_t yes;
bool normalize = false;
int reference = 0;
bool similarity_mode = false;
@ -352,11 +339,10 @@ int obi_clean(const char* dms_name,
obidebug(1, "\nError allocating memory for the array of sample counts, size: %lld", seq_count * sample_count * sizeof(int));
return -1;
}
for (sample=0; sample < sample_count; sample++)
for (samp=0; samp < sample_count; samp++)
{
sample_count_array = complete_sample_count_array+(sample*seq_count);
for (i=0; i<seq_count; i++)
sample_count_array[i] = obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, i, sample);
for (k=0; k<seq_count; k++)
complete_sample_count_array[k+(samp*seq_count)] = obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, k, samp);
}
// Allocate arrays for blobs otherwise reading in mapped files takes longer
@ -367,14 +353,15 @@ int obi_clean(const char* dms_name,
obidebug(1, "\nError allocating memory for the array of blobs");
return -1;
}
for (i=0; i<seq_count; i++)
for (k=0; k<seq_count; k++)
{
blob_array[i] = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, i, 0);
blob_array[k] = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, k, 0);
}
// Allocate alignment result array (byte at 0 if not aligned yet,
// 1 if sequence at index has a similarity above the threshold with the current sequence,
// 2 if sequence at index has a similarity below the threshold with the current sequence)
//alignment_result_array = (byte_t*) calloc(thread_count*seq_count, sizeof(byte_t));
alignment_result_array = (byte_t*) calloc(seq_count, sizeof(byte_t));
if (alignment_result_array == NULL)
{
@ -384,13 +371,13 @@ int obi_clean(const char* dms_name,
}
// Initialize all sequences to singletons or NA if no sequences in that sample
for (i=0; i<seq_count; i++)
for (k=0; k<seq_count; k++)
{
for (sample=0; sample < sample_count; sample++)
for (samp=0; samp < sample_count; samp++)
{
if (obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, i, sample) != OBIInt_NA) // Only initialize samples where there are some sequences
if (obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, k, samp) != OBIInt_NA) // Only initialize samples where there are some sequences
{
if (obi_set_char_with_elt_idx_and_col_p_in_view(o_view, status_column, i, sample, 's') < 0)
if (obi_set_char_with_elt_idx_and_col_p_in_view(o_view, status_column, k, samp, 's') < 0)
{
obidebug(1, "\nError initializing all sequences to singletons");
return -1;
@ -399,38 +386,77 @@ int obi_clean(const char* dms_name,
}
}
Obi_blob_p blob1;
index_t i;
byte_t* ali_result_array = alignment_result_array;
for (i=0; i< (seq_count-1); i++)
{
if (i%1000 == 0)
{
p = (i/(float)seq_count)*100;
p = (i/(float)(seq_count/(float)thread_count))*100;
fprintf(stderr,"\rDone : %f %% ",p);
}
// Get first sequence
blob1 = blob_array[i];
// blob1 = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, i, 0); // slower
// blob1 = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, i, 0); // slower
if (blob1 == NULL)
{
obidebug(1, "\nError retrieving sequences to align");
return -1;
stop = true;
}
#pragma omp parallel default(none) \
shared(ali_result_array, thread_count, seq_count, blob_array, complete_sample_count_array, alignment_result_array, stop, blob1, i, \
obi_errno, stderr, max_ratio, iseq_column, i_view, similarity_mode, reference, normalize, threshold, ktable, status_column, o_view, sample_count)
//private(ali_result_array, thread_id, i, j, p, blob1, blob2, s1_count, s2_count, \
sample_count_array, sample, yes, no, above_threshold, ali_length, lcs_length, ali_result, score)
{
//byte_t* ali_result_array = NULL;
int thread_id = 0;
index_t j;
float p;
Obi_blob_p blob2;
int s1_count;
int s2_count;
int* sample_count_array;
int sample;
byte_t no;
byte_t yes;
double score;
int lcs_min;
bool above_threshold;
int lcs_length;
int ali_length;
byte_t ali_result;
// #ifdef _OPENMP
// thread_id = omp_get_thread_num();
// ali_result_array = alignment_result_array+thread_id;
// #else
// ali_result_array = alignment_result_array;
// #endif
#pragma omp for schedule(dynamic, sample_count/thread_count)
for (sample=0; sample < sample_count; sample++)
{
sample_count_array = complete_sample_count_array+(sample*seq_count);
// Get count for this sample
s1_count = sample_count_array[i];
//s1_count = obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, i, sample); // slower
#pragma omp parallel shared(i, seq_count, s1_count, sample, blob_array, sample_count_array, alignment_result_array, stop) \
private(j, blob2, s2_count, yes, no, above_threshold, ali_result, score)
{
#pragma omp for schedule(dynamic, 100)
for (j=i+1; j < seq_count; j++)
{
//fprintf(stderr, "\nthread=%d, i=%d, sample=%d, j=%d", omp_get_thread_num(),i,sample,j);
// Get second sequence
blob2 = blob_array[j];
// blob2 = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, j, 0); // slower
@ -453,7 +479,7 @@ int obi_clean(const char* dms_name,
yes = 0;
no = 0;
above_threshold = false;
ali_result = alignment_result_array[j];
ali_result = ali_result_array[j];
if (ali_result > 0) // already aligned
{
if (ali_result == 2)
@ -483,7 +509,7 @@ int obi_clean(const char* dms_name,
{
if (yes == 0)
// Set ali result as above the threshold (value 1)
alignment_result_array[j] = 1;
ali_result_array[j] = 1;
// Might be worth having arrays to read values too for some datasets but unlikely
// label as head or internal
@ -513,17 +539,19 @@ int obi_clean(const char* dms_name,
}
}
}
else if (no == 0)
// Set ali result as above the threshold (value 2)
alignment_result_array[j] = 2;
ali_result_array[j] = 2;
}
}
}
if (stop)
return -1;
}
// Reset ali result array to 0
memset(alignment_result_array, 0, seq_count);
memset(ali_result_array, 0, seq_count);
}
}
free_kmer_tables(ktable, seq_count);
@ -533,6 +561,9 @@ int obi_clean(const char* dms_name,
fprintf(stderr, "\n");
if (stop)
return -1;
if (heads_only)
{
line_selection = malloc((o_view->infos)->line_count * sizeof(index_t));
@ -545,11 +576,11 @@ int obi_clean(const char* dms_name,
l=0;
}
for (i=0; i<seq_count; i++)
for (k=0; k<seq_count; k++)
{
if (i%1000 == 0)
if (k%1000 == 0)
{
p = (i/(float)(seq_count))*100;
p = (k/(float)(seq_count))*100;
fprintf(stderr, "\rAnnotating : %f %% ",p);
}
@ -559,16 +590,16 @@ int obi_clean(const char* dms_name,
singleton_count = 0;
ind_sample_count = 0;
for (sample=0; sample < sample_count; sample++)
for (samp=0; samp < sample_count; samp++)
{
// Check if head or singleton in at least one sample
status = obi_get_char_with_elt_idx_and_col_p_in_view(o_view, status_column, i, sample);
status = obi_get_char_with_elt_idx_and_col_p_in_view(o_view, status_column, k, samp);
if ((!head) && ((status == 'h') || (status == 's')))
{
head = true;
if (heads_only)
{
line_selection[l] = i;
line_selection[l] = k;
l++;
}
}
@ -593,15 +624,15 @@ int obi_clean(const char* dms_name,
if (!heads_only || (heads_only && head)) // Label only if sequence is going to be kept in final view
{
if (obi_set_bool_with_elt_idx_and_col_p_in_view(o_view, head_column, i, 0, head) < 0)
if (obi_set_bool_with_elt_idx_and_col_p_in_view(o_view, head_column, k, 0, head) < 0)
return -1;
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, singletoncount_column, i, 0, singleton_count) < 0)
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, singletoncount_column, k, 0, singleton_count) < 0)
return -1;
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, internalcount_column, i, 0, internal_count) < 0)
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, internalcount_column, k, 0, internal_count) < 0)
return -1;
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, headcount_column, i, 0, head_count) < 0)
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, headcount_column, k, 0, head_count) < 0)
return -1;
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, samplecount_column, i, 0, ind_sample_count) < 0)
if (obi_set_int_with_elt_idx_and_col_p_in_view(o_view, samplecount_column, k, 0, ind_sample_count) < 0)
return -1;
}
}