ecotag: added separate threshold for minimum circle identity (and switch

to version 3.0.0b35

python/obitools3/commands/ecotag.pyx

@@ -41,6 +41,17 @@ def addOptions(parser):
                       help="Minimum identity to consider for assignment, as a normalized identity, e.g. 0.95 for an identity of 95%%. "
                            "Default: 0.00 (no threshold).")
 
+    group.add_argument('--minimum-circle','-c',
+                      action="store", dest="ecotag:bubble_threshold",
+                      metavar='<CIRCLE_THRESHOLD>',
+                      default=0.99,
+                      type=float,
+                      help="Minimum identity considered for the assignment circle "
+                           "(sequence is assigned to the LCA of all sequences within a similarity circle of the best matches; "
+                           "the threshold for this circle is the highest value between <CIRCLE_THRESHOLD> and the best assignment score found). "
+                           "Give value as a normalized identity, e.g. 0.95 for an identity of 95%%. "
+                           "Default: 0.99.")
+
 def run(config):
         
     DMS.obi_atexit()
@@ -66,9 +77,8 @@ def run(config):
     ref_view_name = ref[1]
 
     # Check that the threshold demanded is greater than or equal to the threshold used to build the reference database
-    if config['ecotag']['threshold'] < eval(ref_dms[ref_view_name].comments["ref_db_threshold"]) :
-        print("Error: The threshold demanded (%f) is lower than the threshold used to build the reference database (%f).", 
-              config['ecotag']['threshold'], ref_dms[ref_view_name].comments["ref_db_threshold"])
+    if config['ecotag']['bubble_threshold'] < eval(ref_dms[ref_view_name].comments["ref_db_threshold"]) :
+        raise Exception(f"Error: The threshold demanded ({config['ecotag']['bubble_threshold']}) is lower than the threshold used to build the reference database ({float(ref_dms[ref_view_name].comments['ref_db_threshold'])}).")
     
     # Open the output: only the DMS
     output = open_uri(config['obi']['outputURI'],
@@ -113,8 +123,9 @@ def run(config):
     if obi_ecotag(i_dms.name_with_full_path, tobytes(i_view_name), \
                   ref_dms.name_with_full_path, tobytes(ref_view_name), \
                   taxo_dms.name_with_full_path, tobytes(taxonomy_name), \
-                  tobytes(o_view_name), comments, 
-                  config['ecotag']['threshold']) < 0:
+                  tobytes(o_view_name), comments, \
+                  config['ecotag']['threshold'], \
+                  config['ecotag']['bubble_threshold']) < 0:
         raise Exception("Error running ecotag")
     
     # If the input and output DMS are not the same, export result view to output DMS

python/obitools3/dms/capi/obiecotag.pxd

@@ -11,4 +11,5 @@ cdef extern from "obi_ecotag.h" nogil:
                    const char* taxonomy_name,
                    const char* output_view_name,
                    const char* output_view_comments,
-                   double ecotag_threshold)
+                   double ecotag_threshold,
+                   double bubble_threshold)

python/obitools3/version.py

@@ -1,5 +1,5 @@
 major = 3
 minor = 0
-serial= '0b34'
+serial= '0b35'
 
 version ="%d.%d.%s" % (major,minor,serial)