Testing RAM instead of mmap for blob alignment

exceptions in Python>=3.7

python/obitools3/commands/ngsfilter.pyx

@@ -24,6 +24,9 @@ from cpython.exc cimport PyErr_CheckSignals
 from io import BufferedWriter
 
 
+MAX_PAT_LEN = 31
+
+
 __title__="Assign sequence records to the corresponding experiment/sample based on DNA tags and primers"
 
 
@@ -84,6 +87,8 @@ class Primer:
         @type direct:
         '''
         
+        assert len(sequence) <= MAX_PAT_LEN, "Primer %s is too long, 31 bp max" % sequence
+        
         assert sequence not in Primer.collection        \
             or Primer.collection[sequence]==taglength,  \
             "Primer %s must always be used with tags of the same length" % sequence

python/obitools3/parsers/fastq.pyx

@@ -103,7 +103,11 @@ def fastqWithQualityIterator(lineiterator,
         yield seq
 
         read+=1
+        try:
             hline = next(i)
+        except StopIteration:
+            return
+
     
 
 def fastqWithoutQualityIterator(lineiterator, 
@@ -174,5 +178,7 @@ def fastqWithoutQualityIterator(lineiterator,
         yield seq
     
         read+=1
+        try:
             hline = next(i)
-        
+        except StopIteration:
+            return        

python/obitools3/parsers/tab.pyx

@@ -99,7 +99,10 @@ def tabIterator(lineiterator,
         
         read+=1
         
+        try:
             line = next(iterator)
+        except StopIteration:
+            return 
     
         
         

python/obitools3/version.py

@@ -1,5 +1,5 @@
 major = 3
 minor = 0
-serial= '1b22'
+serial= '1b25'
 
 version ="%d.%d.%s" % (major,minor,serial)

src/obi_ecopcr.c

@@ -365,8 +365,6 @@ static int print_seq(Obiview_p i_view, Obiview_p o_view,
 
 	int32_t i;
 
-	// TODO add check for primer longer than MAX_PAT_LEN (32)
-
 	// Get sequence id
 	seq_id = obi_get_str_with_elt_idx_and_col_p_in_view(i_view, i_id_column, i_idx, 0);
 
@@ -751,6 +749,20 @@ int obi_ecopcr(const char* i_dms_name,
 	o1c = complementPattern(o1);
 	o2c = complementPattern(o2);
 
+	// check for primers equal or longer than MAX_PAT_LEN (32)
+	if (strlen(primer1) >= MAX_PAT_LEN)
+	{
+		obi_set_errno(OBI_ECOPCR_ERROR);
+		obidebug(1, "\nError: first primer is too long, needs to be < 32bp (%s)", primer1);
+		return -1;
+	}
+	if (strlen(primer2) >= MAX_PAT_LEN)
+	{
+		obi_set_errno(OBI_ECOPCR_ERROR);
+		obidebug(1, "\nError: second primer is too long, needs to be < 32bp (%s)", primer2);
+		return -1;
+	}
+
 	// Open input DMS
 	i_dms = obi_open_dms(i_dms_name, false);
 	if (i_dms == NULL)

src/obi_lcs.c

@@ -10,8 +10,9 @@
  */
 
 //#define OMP_SUPPORT // TODO
-#ifdef OMP_SUPPORT
+#ifdef _OPENMP
 #include <omp.h>
+#include <pthread.h>
 #endif
 
 #include <stdlib.h>
@@ -407,10 +408,15 @@ int obi_lcs_align_one_column(const char* dms_name,
 	int             lcs_length;
 	int             ali_length;
 	Kmer_table_p    ktable;
+	Obi_blob_p      blob1_mapped;
+	Obi_blob_p   	blob2_mapped;
 	Obi_blob_p      blob1;
 	Obi_blob_p      blob2;
+	int				blob1_size;
+	int             blob2_size;
 	int				lcs_min;
 	index_t         seq_elt_idx;
+	bool			stop = false;
 
 	OBIDMS_p        dms = NULL;
 	Obiview_p       seq_view = NULL;
@@ -568,10 +574,16 @@ int obi_lcs_align_one_column(const char* dms_name,
 
 	seq_count = (seq_view->infos)->line_count;
 
-	#ifdef OMP_SUPPORT
+//	#ifdef _OPENMP
-	omp_set_num_threads(thread_count);
+//	int max_threads = omp_get_max_threads();
-	#pragma omp parallel for
+//	if ((thread_count == -1) || (thread_count > max_threads))
-	#endif
+//		thread_count = max_threads;
+//	omp_set_num_threads(thread_count);
+//	fprintf(stderr, "Running on %d thread(s)\n", thread_count);
+//	pthread_mutex_t mutex;
+//	if (pthread_mutex_init(&mutex, NULL) < 0)
+//		return -1;
+//	#endif
 
 	for (i=0; i < (seq_count - 1); i++)
 	{
@@ -585,23 +597,45 @@ int obi_lcs_align_one_column(const char* dms_name,
 		id1_idx = obi_get_index_with_elt_idx_and_col_p_in_view(seq_view, id_column, i, 0);	// TODO Could there be multiple IDs per line?
 		// Get first sequence and its index
 		seq1_idx = obi_get_index_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, i, seq_elt_idx);
-		blob1 = obi_get_blob_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, i, seq_elt_idx);
+		blob1_mapped = obi_get_blob_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, i, seq_elt_idx);
+		if (blob1_mapped == NULL)
+		{
+			obidebug(1, "\nError retrieving sequences to align");
+			return -1;
+		}
+		blob1_size = obi_blob_sizeof(blob1_mapped);
+		blob1 = (Obi_blob_p) malloc(blob1_size);
 		if (blob1 == NULL)
 		{
 			obidebug(1, "\nError retrieving sequences to align");
 			return -1;
 		}
+		blob1 = memcpy(blob1, blob1_mapped, blob1_size);
 
+//#pragma omp parallel shared(blob1_mapped, blob1, blob1_size, id1_idx, seq1_idx, stop, seq_count, output_view, k, ktable, \
+//		   idx1_column, idx2_column, i, id1_column, id2_column, print_seq, seq1_column, seq2_column, \
+//		   print_count, count1_column, count2_column, ali_length_column, lcs_length_column, score_column, reference, normalize, similarity_mode) \
+//		private(blob2_mapped, blob2, blob2_size, j, id2_idx, seq2_idx, count2, count1, score, ali_length, lcs_length)
+//{
+//		#pragma omp for schedule(dynamic, seq_count/thread_count + (seq_count % thread_count != 0))  // Avoid 0 which blocks the program
 		for (j=i+1; j < seq_count; j++)
 		{
 			// Get second sequence and its index
 			seq2_idx = obi_get_index_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, j, seq_elt_idx);
-			blob2 = obi_get_blob_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, j, seq_elt_idx);
+			blob2_mapped = obi_get_blob_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, j, seq_elt_idx);
+			if (blob2_mapped == NULL)
+			{
+				obidebug(1, "\nError retrieving sequences to align");
+				stop = true;
+			}
+			blob2_size = obi_blob_sizeof(blob2_mapped);
+			blob2 = (Obi_blob_p) malloc(blob2_size);
 			if (blob2 == NULL)
 			{
 				obidebug(1, "\nError retrieving sequences to align");
-				return -1;
+				stop = true;
 			}
+			blob2 = memcpy(blob2, blob2_mapped, blob2_size);
 
 			// Check if the sequences are identical in a quick way (same index in the same indexer)
 			if (obi_get_index_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, i, seq_elt_idx) == obi_get_index_with_elt_idx_and_col_p_in_view(seq_view, iseq_column, j, seq_elt_idx))
@@ -624,6 +658,8 @@ int obi_lcs_align_one_column(const char* dms_name,
 					score = obiblob_sse_banded_lcs_align(blob1, blob2, threshold, normalize, reference, similarity_mode, &lcs_length, &ali_length);
 			}
 
+			free(blob2);
+
 			if ((score >= 0) && (((normalize || similarity_mode) && (score >= threshold)) || ((!similarity_mode && !normalize) && (score <= threshold))))
 			{	// Print result
 
@@ -637,6 +673,10 @@ int obi_lcs_align_one_column(const char* dms_name,
 					count2 = obi_get_int_with_elt_idx_and_col_p_in_view(seq_view, i_count_column, j, 0);
 				}
 
+//				#ifdef _OPENMP
+//				if (pthread_mutex_lock(&mutex) < 0)
+//					stop = true;
+//				#endif
 				if (print_alignment_result(output_view, k,
 										   idx1_column, idx2_column, i, j,
 										   id1_column, id2_column, id1_idx, id2_idx,
@@ -646,15 +686,24 @@ int obi_lcs_align_one_column(const char* dms_name,
 										   lcs_length_column, lcs_length,
 										   score_column, score,
 										   reference, normalize, similarity_mode) < 0)
-					return -1;
+					stop = true;
+//				#ifdef _OPENMP
+//				if (pthread_mutex_unlock(&mutex) < 0)
+//					stop = true;
+//				#endif
 
 				k++;
 			}
 		}
+//}
+		free(blob1);
 	}
 
 	fprintf(stderr,"\rDone : 100 %%         \n");
 
+	//fprintf(stderr,"\nstop=%d\n", stop);
+
+
 	// Close views
 	if (obi_save_and_close_view(seq_view) < 0)
 	{
@@ -675,6 +724,11 @@ int obi_lcs_align_one_column(const char* dms_name,
 
 	free_kmer_tables(ktable, seq_count);
 
+//	#ifdef _OPENMP
+//	if (pthread_mutex_destroy(&mutex) < 0)
+//		return -1;
+//	#endif
+
 	return 0;
 }
 
@@ -1087,4 +1141,3 @@ int obi_lcs_align_two_columns(const char* dms_name,
 
 	return 0;
 }
-