mirror of
https://github.com/metabarcoding/obitools4.git
synced 2025-06-29 16:20:46 +00:00
change the model for representing paired reads and extend its usage to other commands
This commit is contained in:
@ -46,6 +46,7 @@ type _IBioSequence struct {
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batch_size int32
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sequence_format string
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finished *abool.AtomicBool
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paired bool
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}
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type IBioSequence struct {
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@ -73,6 +74,7 @@ func MakeIBioSequence(sizes ...int) IBioSequence {
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batch_size: -1,
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sequence_format: "",
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finished: abool.New(),
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paired: false,
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}
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waiting := sync.WaitGroup{}
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@ -199,6 +201,11 @@ func (iterator IBioSequence) Split() IBioSequence {
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i.lock = &lock
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newIter := IBioSequence{&i}
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if iterator.IsPaired() {
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newIter.MarkAsPaired()
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}
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return newIter
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}
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@ -270,6 +277,7 @@ func (iterator IBioSequence) Finished() bool {
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return iterator.pointer.finished.IsSet()
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}
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// Sorting the batches of sequences.
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func (iterator IBioSequence) SortBatches(sizes ...int) IBioSequence {
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buffsize := iterator.BufferSize()
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@ -311,6 +319,10 @@ func (iterator IBioSequence) SortBatches(sizes ...int) IBioSequence {
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newIter.Done()
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}()
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if iterator.IsPaired() {
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newIter.MarkAsPaired()
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}
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return newIter
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}
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@ -321,6 +333,11 @@ func (iterator IBioSequence) Concat(iterators ...IBioSequence) IBioSequence {
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return iterator
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}
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allPaired := iterator.IsPaired()
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for _, i := range iterators {
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allPaired = allPaired && i.IsPaired()
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}
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buffsize := iterator.BufferSize()
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newIter := MakeIBioSequence(buffsize)
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@ -357,6 +374,10 @@ func (iterator IBioSequence) Concat(iterators ...IBioSequence) IBioSequence {
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newIter.Done()
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}()
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if allPaired {
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newIter.MarkAsPaired()
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}
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return newIter
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}
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@ -368,6 +389,12 @@ func (iterator IBioSequence) Pool(iterators ...IBioSequence) IBioSequence {
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return iterator
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}
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allPaired := iterator.IsPaired()
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for _, i := range iterators {
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allPaired = allPaired && i.IsPaired()
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}
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nextCounter := goutils.AtomicCounter()
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buffsize := iterator.BufferSize()
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newIter := MakeIBioSequence(buffsize)
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@ -392,6 +419,10 @@ func (iterator IBioSequence) Pool(iterators ...IBioSequence) IBioSequence {
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go ff(i)
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}
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if allPaired {
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newIter.MarkAsPaired()
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}
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return newIter
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}
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@ -441,6 +472,10 @@ func (iterator IBioSequence) Rebatch(size int, sizes ...int) IBioSequence {
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}()
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if iterator.IsPaired() {
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newIter.MarkAsPaired()
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}
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return newIter
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}
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@ -492,47 +527,6 @@ func (iterator IBioSequence) Count(recycle bool) (int, int, int) {
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return variants, reads, nucleotides
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}
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func (iterator IBioSequence) PairWith(reverse IBioSequence,
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sizes ...int) IPairedBioSequenceBatch {
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buffsize := iterator.BufferSize()
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batchsize := 5000
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if len(sizes) > 0 {
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batchsize = sizes[0]
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}
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if len(sizes) > 1 {
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buffsize = sizes[1]
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}
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iterator = iterator.Rebatch(batchsize)
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reverse = reverse.Rebatch(batchsize)
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newIter := MakeIPairedBioSequenceBatch(buffsize)
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newIter.Add(1)
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go func() {
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newIter.WaitAndClose()
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log.Println("End of association of paired reads")
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}()
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log.Println("Start association of paired reads")
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go func() {
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for iterator.Next() {
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if !reverse.Next() {
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log.Panicln("Etrange reverse pas prêt")
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}
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newIter.Channel() <- MakePairedBioSequenceBatch(iterator.Get(),
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reverse.Get())
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}
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newIter.Done()
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}()
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return newIter
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}
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// A function that takes a predicate and returns two IBioSequenceBatch iterators.
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// Sequences extracted from the input iterator are distributed among both the
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// iterator following the predicate value.
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@ -599,6 +593,10 @@ func (iterator IBioSequence) DivideOn(predicate obiseq.SequencePredicate,
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falseIter.Done()
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}()
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if iterator.IsPaired() {
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trueIter.MarkAsPaired()
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falseIter.MarkAsPaired()
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}
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return trueIter, falseIter
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}
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@ -654,6 +652,71 @@ func (iterator IBioSequence) FilterOn(predicate obiseq.SequencePredicate,
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go ff(iterator)
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if iterator.IsPaired() {
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trueIter.MarkAsPaired()
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}
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return trueIter.Rebatch(size)
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}
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func (iterator IBioSequence) FilterAnd(predicate obiseq.SequencePredicate,
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size int, sizes ...int) IBioSequence {
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buffsize := iterator.BufferSize()
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nworkers := 4
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if len(sizes) > 0 {
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nworkers = sizes[0]
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}
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if len(sizes) > 1 {
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buffsize = sizes[1]
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}
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trueIter := MakeIBioSequence(buffsize)
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trueIter.Add(nworkers)
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go func() {
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trueIter.WaitAndClose()
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}()
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ff := func(iterator IBioSequence) {
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// iterator = iterator.SortBatches()
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for iterator.Next() {
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seqs := iterator.Get()
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slice := seqs.slice
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j := 0
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for _, s := range slice {
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good := predicate(s)
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if s.IsPaired() {
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good = good && predicate(s.PairedWith())
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}
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if good {
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slice[j] = s
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j++
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} else {
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s.Recycle()
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}
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}
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seqs.slice = slice[:j]
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trueIter.pointer.channel <- seqs
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}
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trueIter.Done()
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}
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for i := 1; i < nworkers; i++ {
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go ff(iterator.Split())
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}
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go ff(iterator)
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if iterator.IsPaired() {
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trueIter.MarkAsPaired()
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}
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return trueIter.Rebatch(size)
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}
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@ -673,13 +736,14 @@ func (iterator IBioSequence) Load() obiseq.BioSequenceSlice {
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// It takes a slice of BioSequence objects, and returns an iterator that will return batches of
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// BioSequence objects
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func IBatchOver(data obiseq.BioSequenceSlice,
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size int, sizes ...int) IBioSequence {
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buffsize := 0
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if len(sizes) > 0 {
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buffsize = sizes[1]
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buffsize = sizes[0]
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}
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newIter := MakeIBioSequence(buffsize)
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@ -706,5 +770,8 @@ func IBatchOver(data obiseq.BioSequenceSlice,
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newIter.Done()
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}()
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if data.IsPaired() {
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newIter.MarkAsPaired()
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}
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return newIter
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}
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