mirror of
https://github.com/metabarcoding/obitools4.git
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many changes ;-)
Former-commit-id: cb4aea844e960e4af4dc673ebc8eec49a7d12b13
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doc/.DS_Store
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doc/book/.ipynb_checkpoints/Untitled-checkpoint.ipynb
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doc/book/.ipynb_checkpoints/Untitled-checkpoint.ipynb
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{
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"cells": [],
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"metadata": {},
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"nbformat": 4,
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"nbformat_minor": 5
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}
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doc/book/Untitled.ipynb
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doc/book/Untitled.ipynb
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{
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"cells": [],
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"metadata": {},
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"nbformat": 4,
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"nbformat_minor": 5
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}
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doc/book/wolf_data/sort_gb_order.sh
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doc/book/wolf_data/sort_gb_order.sh
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#!/bin/bash
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#!/bin/bash
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#OAR -n gbsort
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##OAR --array 50
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##OAR --array-param-file 50_first.tsv
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#OAR --project phyloalps
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#OAR -l nodes=1/core=10,walltime=24:00:00
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#OAR -O gbsort.%jobid%.log
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#OAR -E gbsort.%jobid%.log
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# /silenus/PROJECTS/pr-phyloalps/coissac
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# /bettik/LECA/ENVIRONMENT/data/biodatabase/genbank
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#
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# Used resources URLs
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#
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NCBIURL="https://ftp.ncbi.nlm.nih.gov/" # NCBI Web site URL
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GBURL="${NCBIURL}genbank/" # Directory of Genbank flat files
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TAXOURL="${NCBIURL}pub/taxonomy/taxdump.tar.gz" # NCBI Taxdump
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LOGFILE="download.log"
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#
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# List of downloaded Genbank divisions
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#
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DIV="bct|inv|mam|phg|pln|pri|rod|vrl|vrt"
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############################
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#
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# Functions
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#
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############################
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pattern_at_rank() {
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local taxo="$1"
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local rank="$2"
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echo "^($(awk -F "|" -v rank="$rank" 'BEGIN {
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ORS="|";
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rank="\t" rank "\t"
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}
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($3 ~ rank) {sub(/^[ \t]+/,"",$1);
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sub(/[ \t]+$/,"",$1);
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print $1}
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' "${taxo}/nodes.dmp" \
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| sed 's/|$//'))$"
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}
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GBDIR=$1
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#
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# Extrate from the web site the current Genbank release number
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# end create the corresponding directory
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#
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echo "Looking at current Genbank release number"
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GB_Release_Number=$(for r in $(ls -d "${GBDIR}/Release-"* ); do
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basename $r;
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done \
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| sort -r \
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| head -1 \
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| sed 's/^Release-//')
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GB_Release_Number=251
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echo "identified latest release number is : ${GB_Release_Number}"
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GBSOURCE="${GBDIR}/Release-${GB_Release_Number}"
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mkdir -p "Release-${GB_Release_Number}"
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cd "Release-${GB_Release_Number}" || exit
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#
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# Download the current NCBI taxonomy
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#
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mkdir -p "ncbitaxo"
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if [[ ! -f ncbitaxo/nodes.dmp ]] || [[ ! -f ncbitaxo/names.dmp ]] ; then
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curl "${TAXOURL}" \
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| tar -C "ncbitaxo" -zxf -
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fi
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for f in $(ls -1 "${GBSOURCE}/"*.seq.gz ) ; do
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echo "PROCESSING : $f saved into $fasta" $(pwd)
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obiannotate --genbank -t ncbitaxo \
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--with-taxon-at-rank kingdom \
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--with-taxon-at-rank superkingdom \
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--with-taxon-at-rank phylum\
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--with-taxon-at-rank order \
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--with-taxon-at-rank family \
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--with-taxon-at-rank genus \
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-S division='"misc-@-0"' \
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-S section='"misc-@-0"' \
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"$f" \
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| obigrep -A genus_taxid -A family_taxid \
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| obigrep -p 'annotations.genus_taxid > 0 && annotations.family_taxid > 0' \
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-p 'annotations.phylum_taxid > 0 || annotations.order_taxid > 0' \
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| obiannotate -p 'annotations.superkingdom_taxid > 0' \
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-S division='printf("%s-S-%d",subspc(annotations.superkingdom_name),annotations.superkingdom_taxid)' \
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| obiannotate -p 'annotations.kingdom_taxid > 0' \
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-S division='printf("%s-K-%d",subspc(annotations.kingdom_name),annotations.kingdom_taxid)' \
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| obiannotate -p 'annotations.phylum_taxid > 0' \
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-S section='printf("%s-P-%d",subspc(annotations.phylum_name),annotations.phylum_taxid)' \
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| obiannotate -p 'annotations.order_taxid > 0' \
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-S section='printf("%s-O-%d",subspc(annotations.order_name),annotations.order_taxid)' \
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| obidistribute -Z -A -p "%s.fasta" -c section -d division
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done
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