Replace MakeBioSequence call by NewBioSequence call,

Implements a new file format guesser
Adds some more API doc


Former-commit-id: 9837bf1c28beca6ddb599b367f93548950ba83c1
This commit is contained in:
2023-08-30 19:59:46 +02:00
parent c2533667b2
commit 3f8c0d6a2f
7 changed files with 582 additions and 116 deletions

View File

@ -2,11 +2,14 @@ package obiformats
import (
"bufio"
gzip "github.com/klauspost/pgzip"
"bytes"
"io"
"os"
"path"
"strings"
"regexp"
"github.com/gabriel-vasile/mimetype"
gzip "github.com/klauspost/pgzip"
log "github.com/sirupsen/logrus"
@ -14,36 +17,89 @@ import (
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiutils"
)
func GuessSeqFileType(firstline string) string {
switch {
case strings.HasPrefix(firstline, "#@ecopcr-v2"):
return "ecopcr"
case strings.HasPrefix(firstline, "#"):
return "ecopcr"
case strings.HasPrefix(firstline, ">"):
return "fasta"
case strings.HasPrefix(firstline, "@"):
return "fastq"
case strings.HasPrefix(firstline, "ID "):
return "embl"
case strings.HasPrefix(firstline, "LOCUS "):
return "genbank"
// Special case for genbank release files
// I hope it is enougth stringeant
case strings.HasSuffix(firstline, " Genetic Se"):
return "genbank"
default:
return "unknown"
// OBIMimeTypeGuesser is a function that takes an io.Reader as input and guesses the MIME type of the data.
// It uses several detectors to identify specific file formats, such as FASTA, FASTQ, ecoPCR2, GenBank, and EMBL.
// The function reads data from the input stream and analyzes it using the mimetype library.
// It then returns the detected MIME type, a modified reader with the read data, and any error encountered during the process.
//
// The following file types are recognized:
// - "text/ecopcr": if the first line starts with "#@ecopcr-v2".
// - "text/fasta": if the first line starts with ">".
// - "text/fastq": if the first line starts with "@".
// - "text/embl": if the first line starts with "ID ".
// - "text/genbank": if the first line starts with "LOCUS ".
// - "text/genbank" (special case): if the first line "Genetic Sequence Data Bank" (for genbank release files).
// - "text/csv"
//
// Parameters:
// - stream: An io.Reader representing the input stream to read data from.
//
// Returns:
// - *mimetype.MIME: The detected MIME type of the data.
// - io.Reader: A modified reader with the read data.
// - error: Any error encountered during the process.
func OBIMimeTypeGuesser(stream io.Reader) (*mimetype.MIME, io.Reader, error) {
fastaDetector := func(raw []byte, limit uint32) bool {
ok, err := regexp.Match("^>[^ ]", raw)
return ok && err == nil
}
fastqDetector := func(raw []byte, limit uint32) bool {
ok, err := regexp.Match("^@[^ ]", raw)
return ok && err == nil
}
ecoPCR2Detector := func(raw []byte, limit uint32) bool {
ok := bytes.HasPrefix(raw, []byte("#@ecopcr-v2"))
return ok
}
genbankDetector := func(raw []byte, limit uint32) bool {
ok2 := bytes.HasPrefix(raw, []byte("LOCUS "))
ok1, err := regexp.Match("^[^ ]* +Genetic Sequence Data Bank *\n", raw)
return ok2 || (ok1 && err == nil)
}
emblDetector := func(raw []byte, limit uint32) bool {
ok := bytes.HasPrefix(raw, []byte("ID "))
return ok
}
mimetype.Lookup("text/plain").Extend(fastaDetector, "text/fasta", ".fasta")
mimetype.Lookup("text/plain").Extend(fastqDetector, "text/fastq", ".fastq")
mimetype.Lookup("text/plain").Extend(ecoPCR2Detector, "text/ecopcr2", ".ecopcr")
mimetype.Lookup("text/plain").Extend(genbankDetector, "text/genbank", ".seq")
mimetype.Lookup("text/plain").Extend(emblDetector, "text/embl", ".dat")
// Create a buffer to store the read data
buf := make([]byte, 1024*128)
n, err := stream.Read(buf)
if err != nil && err != io.EOF {
return nil, nil, err
}
// Detect the MIME type using the mimetype library
mimeType := mimetype.Detect(buf)
if mimeType == nil {
return nil, nil, err
}
// Create a new reader based on the read data
newReader := io.MultiReader(bytes.NewReader(buf[:n]), stream)
return mimeType, newReader, nil
}
// ReadSequencesFromFile reads sequences from a file and returns an iterator of bio sequences and an error.
//
// Parameters:
// - filename: The name of the file to read the sequences from.
// - options: Optional parameters to customize the reading process.
//
// Returns:
// - obiiter.IBioSequence: An iterator of bio sequences.
// - error: An error if any occurred during the reading process.
func ReadSequencesFromFile(filename string,
options ...WithOption) (obiiter.IBioSequence, error) {
var file *os.File
@ -71,35 +127,28 @@ func ReadSequencesFromFile(filename string,
reader = greader
}
breader := bufio.NewReader(reader)
mime, reader, err := OBIMimeTypeGuesser(reader)
tag, _ := breader.Peek(30)
if len(tag) < 30 {
newIter := obiiter.MakeIBioSequence()
newIter.Close()
return newIter, nil
if err != nil {
return obiiter.NilIBioSequence, err
}
filetype := GuessSeqFileType(string(tag))
log.Debugf("File guessed format : %s (tag: %s)",
filetype, (strings.Split(string(tag), "\n"))[0])
reader = breader
reader = bufio.NewReader(reader)
switch filetype {
case "fastq", "fasta":
switch mime.String() {
case "text/fasta", "text/fastq":
file.Close()
is, err := ReadFastSeqFromFile(filename, options...)
return is, err
case "ecopcr":
case "text/ecopcr2":
return ReadEcoPCR(reader, options...), nil
case "embl":
case "text/embl":
return ReadEMBL(reader, options...), nil
case "genbank":
case "text/genbank":
return ReadGenbank(reader, options...), nil
default:
log.Fatalf("File %s has guessed format %s which is not yet implemented",
filename, filetype)
filename, mime.String())
}
return obiiter.NilIBioSequence, nil