obitools4/pkg/obiformats/genbank_read.go

package obiformats

import (
	"bufio"
	"bytes"
	"io"
	"os"
	"strconv"
	"strings"

	gzip "github.com/klauspost/pgzip"

	log "github.com/sirupsen/logrus"

	"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiiter"
	"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
)

type gbstate int

const (
	inHeader     gbstate = 0
	inEntry      gbstate = 1
	inDefinition gbstate = 2
	inFeature    gbstate = 3
	inSequence   gbstate = 4
)

func _ParseGenbankFile(input <-chan _FileChunk, out obiiter.IBioSequence) {

	state := inHeader

	for chunks := range input {
		log.Debugln("Chunk size", (chunks.raw.(*bytes.Buffer)).Len())
		scanner := bufio.NewScanner(chunks.raw)
		order := chunks.order
		sequences := make(obiseq.BioSequenceSlice, 0, 100)
		id := ""
		scientificName := ""
		defBytes := new(bytes.Buffer)
		featBytes := new(bytes.Buffer)
		seqBytes := new(bytes.Buffer)
		taxid := 1
		nl := 0
		sl := 0
		for scanner.Scan() {
			nl++
			line := scanner.Text()
			switch {
			case state==inDefinition && ! strings.HasPrefix(line, "            "):
				state = inEntry
				fallthrough
			case strings.HasPrefix(line, "LOCUS       "):
				state = inEntry
				id = strings.SplitN(line[12:], " ", 2)[0]
			case strings.HasPrefix(line, "SOURCE      "):
				scientificName = strings.TrimSpace(line[12:])
			case strings.HasPrefix(line, "DEFINITION  "):
				defBytes.WriteString(strings.TrimSpace(line[12:]))
				state = inDefinition
			case strings.HasPrefix(line, "FEATURES    "):
				featBytes.WriteString(line)
				state = inFeature
			case strings.HasPrefix(line, "ORIGIN      "):
				state = inSequence
			case line == "//":
				log.Debugln("Total lines := ", nl)
				sequence := obiseq.NewBioSequence(id,
					bytes.ToLower(seqBytes.Bytes()),
					defBytes.String())
				state = inHeader

				sequence.SetFeatures(featBytes.Bytes())

				annot := sequence.Annotations()
				annot["scientific_name"] = scientificName
				annot["taxid"] = taxid
				// log.Println(FormatFasta(sequence, FormatFastSeqJsonHeader))
				sequences = append(sequences, sequence)
				defBytes = new(bytes.Buffer)
				featBytes = new(bytes.Buffer)
				seqBytes = new(bytes.Buffer)
				nl=0
				sl=0
			default:
				switch state {
				case inDefinition:
					defBytes.WriteByte(' ')
					defBytes.WriteString(strings.TrimSpace(line[5:]))
				case inFeature:
					featBytes.WriteByte('\n')
					featBytes.WriteString(line)
					if strings.HasPrefix(line, `                     /db_xref="taxon:`) {
						taxid, _ = strconv.Atoi(strings.SplitN(line[37:], `"`, 2)[0])
					}
				case inSequence:
					sl++
					parts := strings.SplitN(line[10:], " ", 7)
					lparts := len(parts)
					for i := 0; i < lparts; i++ {
						seqBytes.WriteString(parts[i])
					}
				}
			}

		}
		out.Push(obiiter.MakeBioSequenceBatch(order, sequences))
	}

	out.Done()

}

func ReadGenbank(reader io.Reader, options ...WithOption) obiiter.IBioSequence {
	opt := MakeOptions(options)
	entry_channel := make(chan _FileChunk)

	newIter := obiiter.MakeIBioSequence()

	nworkers := opt.ParallelWorkers()
	newIter.Add(nworkers)

	go func() {
		newIter.WaitAndClose()
	}()

	// for j := 0; j < opt.ParallelWorkers(); j++ {
	for j := 0; j < nworkers; j++ {
		go _ParseGenbankFile(entry_channel, newIter)
	}

	go _ReadFlatFileChunk(reader, entry_channel)

	return newIter
}

func ReadGenbankFromFile(filename string, options ...WithOption) (obiiter.IBioSequence, error) {
	var reader io.Reader
	var greader io.Reader
	var err error

	reader, err = os.Open(filename)
	if err != nil {
		log.Printf("open file error: %+v", err)
		return obiiter.NilIBioSequence, err
	}

	// Test if the flux is compressed by gzip
	//greader, err = gzip.NewReader(reader)
	greader, err = gzip.NewReaderN(reader, 1<<24, 2)
	if err == nil {
		reader = greader
	}

	return ReadGenbank(reader, options...), nil
}