src/obikidxcache/src/index_cache.rs

@@ -119,6 +119,16 @@ impl<'a> IndexCache<'a> {
         })
     }
 
+    /// Iterate over the unitigs of every cached layer, chained — the
+    /// partition/index level of `KmerLayer::iter_unitigs`'s layer/partition/
+    /// index chain, covering whichever scope this cache was opened with
+    /// (one partition, several, or the whole index — see [`new`](Self::new)).
+    /// No extra opening: every layer here is already open, this only
+    /// streams what [`iter`](Self::iter) already gives out.
+    pub fn iter_unitigs(&self) -> impl Iterator<Item = obikseq::Unitig> + '_ {
+        self.iter().flat_map(|l| l.iter_unitigs())
+    }
+
     #[inline]
     pub fn hash(&self, partition: usize, layer: usize, kmer: CanonicalKmer) -> Option<usize> {
         Some(self.get_layer(partition, layer)?.hash(kmer))

src/obikindex/src/layer/content_layer.rs

@@ -311,4 +311,16 @@ impl KmerLayer {
             }
         }
     }
+
+    /// Iterate over the layer's unitigs (whole sequences, not decomposed
+    /// into k-mers) — the "layer level" step of the layer/partition/index
+    /// chain of unitig iterators; see `KmerPartition::iter_unitigs`/
+    /// `KmerIndex::iter_unitigs`.
+    pub fn iter_unitigs(&self) -> crate::layer::mphf_layer::UnitigIter {
+        match self {
+            KmerLayer::Count { layer, .. } => layer.iter_unitigs(),
+            KmerLayer::Presence { layer, .. } => layer.iter_unitigs(),
+            KmerLayer::Empty { .. } => panic!("Layer::iter_unitigs() called on an Empty layer"),
+        }
+    }
 }

src/obikindex/src/layer/mod.rs

@@ -9,5 +9,5 @@ pub(crate) mod utils;
 pub use crate::index::error::{OKIError, OKIResult};
 pub use content_layer::KmerLayer;
 pub use meta::IndexMode;
-pub use mphf_layer::{EvidenceKind, KmerBatchIter, KmerIter, MphfLayer, MphfOnly};
+pub use mphf_layer::{EvidenceKind, KmerBatchIter, KmerIter, MphfLayer, MphfOnly, UnitigIter};
 pub use typed_layer::{HasLayerContent, HasStorageKind, Hit, LayerContent, LayerData, TypedLayer};

src/obikindex/src/layer/mphf_layer.rs

@@ -341,6 +341,16 @@ impl MphfLayer {
             (base, batch)
         })
     }
+
+    /// Iterate over the layer's unitigs (whole reconstructed sequences, not
+    /// decomposed into k-mers), in the physical order of `unitigs.bin`. Same
+    /// `Send + 'static` shape as [`iter_kmers`](Self::iter_kmers), via
+    /// `UnitigFileReader::iter_unitigs_owned`.
+    pub fn iter_unitigs(&self) -> UnitigIter {
+        UnitigIter {
+            inner: Box::new(self.unitigs.iter_unitigs_owned()),
+        }
+    }
 }
 
 // ── Iterator types ────────────────────────────────────────────────────────────
@@ -365,6 +375,24 @@ impl Iterator for KmerIter {
     }
 }
 
+/// Iterator over the unitigs stored in a layer, whole (not decomposed into
+/// k-mers). Produced by [`MphfLayer::iter_unitigs`]. Same ownership shape as
+/// [`KmerIter`] — owns an `Arc<UnitigFileReader>` clone, `Send + 'static`,
+/// streamed from disk.
+pub struct UnitigIter {
+    inner: Box<dyn Iterator<Item = (usize, obikseq::Unitig)> + Send>,
+}
+
+impl Iterator for UnitigIter {
+    type Item = obikseq::Unitig;
+
+    /// Return the next unitig in iteration order (its `chunk_id` dropped —
+    /// use [`Iterator::enumerate`] if the index within the layer is needed).
+    fn next(&mut self) -> Option<Self::Item> {
+        self.inner.next().map(|(_, unitig)| unitig)
+    }
+}
+
 /// Iterator over batches of canonical kmers stored in a layer.
 ///
 /// Produced by [`MphfLayer::iter_kmers_batch`].  Each call to [`next`](Self::next)

src/obikindex/src/layer/typed_layer.rs

@@ -223,6 +223,12 @@ impl<D: LayerData> TypedLayer<D> {
         self.mphf.enumerate_kmers_batch(n)
     }
 
+    /// Iterate over the layer's unitigs (whole sequences, not decomposed
+    /// into k-mers).
+    pub fn iter_unitigs(&self) -> crate::layer::mphf_layer::UnitigIter {
+        self.mphf.iter_unitigs()
+    }
+
     pub fn unitig_writer(out_dir: &Path) -> OKIResult<UnitigFileWriter> {
         MphfLayer::unitig_writer(out_dir)
     }

src/obikmer2/src/cmd/estimate/mod.rs

@@ -0,0 +1,38 @@
+use clap::Args;
+
+use super::index::resolve_approx_params;
+
+#[derive(Args)]
+pub struct EstimateArgs {
+    /// k-mer size used for querying (same as --kmer-size in index)
+    #[arg(short = 'k', long, default_value_t = 31)]
+    pub kmer_size: usize,
+
+    /// Findere z parameter: number of consecutive k-mers that must all match.
+    /// Effective indexed k-mer size is kmer_size - z + 1.
+    #[arg(short = 'z', long, default_value = None)]
+    pub findere_z: Option<u8>,
+
+    /// Fingerprint bits per slot (b).  FP per z-window = 1/2^(b·z).
+    #[arg(long, default_value = None)]
+    pub evidence_bits: Option<u8>,
+
+    /// Target false-positive rate per z-window (e.g. 0.01).
+    #[arg(long, default_value = None)]
+    pub fp: Option<f64>,
+}
+
+pub fn run(args: EstimateArgs) {
+    let (z, b, fp_window) = resolve_approx_params(args.findere_z, args.evidence_bits, args.fp);
+
+    let k_query = args.kmer_size;
+    let k_index = k_query.saturating_sub(z as usize - 1);
+    let fp_kmer = 1.0_f64 / 2_f64.powi(b as i32);
+
+    println!("{:<22} {}", "k (query):",        k_query);
+    println!("{:<22} {}", "k (indexed):",      k_index);
+    println!("{:<22} {}", "z:",                z);
+    println!("{:<22} {}", "evidence bits (b):", b);
+    println!("{:<22} {:.3e}  (1/2^{})",        "FP per k-mer:",    fp_kmer,   b);
+    println!("{:<22} {:.3e}  (1/2^{})",        "FP per z-window:", fp_window, b as u32 * z as u32);
+}

src/obikmer2/src/cmd/mod.rs

@@ -1,3 +1,4 @@
+pub mod estimate;
 pub mod index;
 pub mod merge;
 pub mod superkmer;

src/obikmer2/src/main.rs

@@ -19,6 +19,8 @@ enum Commands {
     Superkmer(cmd::superkmer::SuperkmerArgs),
     /// Merge multiple genome indexes into one
     Merge(cmd::merge::MergeArgs),
+    /// Estimate approximate-evidence false-positive rates for given parameters
+    Estimate(cmd::estimate::EstimateArgs),
 }
 
 fn main() {
@@ -34,5 +36,6 @@ fn main() {
         Commands::Index(args) => cmd::index::run(args),
         Commands::Superkmer(args) => cmd::superkmer::run(args),
         Commands::Merge(args) => cmd::merge::run(args),
+        Commands::Estimate(args) => cmd::estimate::run(args),
     }
 }

src/obiskio/src/unitig_index/reader.rs

@@ -208,6 +208,31 @@ impl UnitigFileReader {
         self.iter_chunks_sequential()
     }
 
+    /// Same streamed sequence as [`iter_unitigs`](Self::iter_unitigs), but
+    /// owning a clone of `self` instead of borrowing it — `Send + 'static`,
+    /// so it can be handed to a threaded consumer without first collecting
+    /// the layer's unitigs into memory. Same shape as
+    /// [`iter_indexed_canonical_kmers_owned`](Self::iter_indexed_canonical_kmers_owned),
+    /// stopping short of decomposing into k-mers.
+    pub fn iter_unitigs_owned(
+        self: &Arc<Self>,
+    ) -> impl Iterator<Item = (usize, Unitig)> + Send + 'static {
+        let this = Arc::clone(self);
+        let k = this.k;
+        let n = this.n_unitigs;
+        let mut offset = 0usize;
+        (0..n).map(move |chunk_id| {
+            let mmap = &*this.mmap;
+            let seql = mmap[offset] as usize + k;
+            let byte_len = (seql + 3) / 4;
+            let bytes = mmap[offset + 1..offset + 1 + byte_len]
+                .to_vec()
+                .into_boxed_slice();
+            offset += 1 + byte_len;
+            (chunk_id, Unitig::new((seql % 4) as u8, bytes))
+        })
+    }
+
     pub fn iter_kmers(&self) -> impl Iterator<Item = Kmer> + '_ {
         self.iter_chunks_sequential()
             .flat_map(|(_, u)| u.into_kmers())