Add option related to agrep match on obigrep and obiannotate

This commit is contained in:
Eric Coissac
2024-09-09 16:52:13 +02:00
parent 65ae82622e
commit 05bf2bfd6c
12 changed files with 167 additions and 34 deletions

View File

@@ -25,7 +25,7 @@ func DeleteAttributesWorker(toBeDeleted []string) obiseq.SeqWorker {
return f
}
func MatchPatternWorker(pattern, name string, errormax int, allowsIndel bool) obiseq.SeqWorker {
func MatchPatternWorker(pattern, name string, errormax int, bothStrand, allowsIndel bool) obiseq.SeqWorker {
pat, err := obiapat.MakeApatPattern(pattern, errormax, allowsIndel)
if err != nil {
log.Fatalf("error in compiling pattern (%s) : %v", pattern, err)
@@ -56,7 +56,7 @@ func MatchPatternWorker(pattern, name string, errormax int, allowsIndel bool) ob
start, end, nerr, matched := pat.BestMatch(apats, 0, s.Len())
if matched {
if matched && start >= 0 && end <= s.Len() {
annot := s.Annotations()
annot[slot] = pattern
@@ -75,7 +75,7 @@ func MatchPatternWorker(pattern, name string, errormax int, allowsIndel bool) ob
} else {
start, end, nerr, matched := cpat.BestMatch(apats, 0, s.Len())
if matched {
if matched && start >= 0 && end <= s.Len() {
annot := s.Annotations()
annot[slot] = pattern
match, err := s.Subsequence(start, end, false)
@@ -328,9 +328,10 @@ func CLIAnnotationWorker() obiseq.SeqWorker {
}
if CLIHasPattern() {
log.Infof("Match pattern %s with %d error", CLIPattern(), CLIPatternError())
log.Infof("Match pattern %s with %d error", CLIPattern(), obigrep.CLIPatternError())
w := MatchPatternWorker(CLIPattern(), CLIHasPatternName(),
CLIPatternError(), CLIPatternInDels())
obigrep.CLIPatternError(), obigrep.CLIPatternBothStrand(),
obigrep.CLIPatternInDels())
annotator = annotator.ChainWorkers(w)
}

View File

@@ -24,8 +24,6 @@ var _setSeqLength = false
var _uniqueID = false
var _ahoCorazick = ""
var _pattern = ""
var _pattern_error = 0
var _pattern_indel = false
var _pattern_name = "pattern"
var _lcaSlot = ""
var _lcaError = 0.0
@@ -62,14 +60,6 @@ func SequenceAnnotationOptionSet(options *getoptions.GetOpt) {
options.Description("specify the name to use as prefix for the slots reporting the match"),
)
options.IntVar(&_pattern_error, "pattern-error", _pattern_error,
options.Description("Maximum number of allowed error during pattern matching"),
)
options.BoolVar(&_pattern_indel, "allows-indels", _pattern_indel,
options.Description("Allows for indel during pattern matching"),
)
options.StringVar(&_lcaSlot, "add-lca-in", _lcaSlot,
options.ArgName("SLOT_NAME"),
options.Description("From the taxonomic annotation of the sequence (taxid slot or merged_taxid slot), "+
@@ -304,14 +294,6 @@ func CLIHasPatternName() string {
return _pattern_name
}
func CLIPatternError() int {
return _pattern_error
}
func CLIPatternInDels() bool {
return _pattern_indel
}
func CLISetTaxonomicPath() bool {
return _taxonomicPath
}

View File

@@ -19,6 +19,8 @@ var __input_fastobi_format__ = false
var __input_ecopcr_format__ = false
var __input_embl_format__ = false
var __input_genbank_format__ = false
var __input_fastq_format__ = false
var __input_fasta_format__ = false
var __output_in_fasta__ = false
var __output_in_fastq__ = false
@@ -56,6 +58,12 @@ func InputOptionSet(options *getoptions.GetOpt) {
options.BoolVar(&__input_genbank_format__, "genbank", __input_genbank_format__,
options.Description("Read data following the Genbank flatfile format."))
options.BoolVar(&__input_fastq_format__, "fastq", __input_fastq_format__,
options.Description("Read data following the fastq format."))
options.BoolVar(&__input_fasta_format__, "fasta", __input_fasta_format__,
options.Description("Read data following the fasta format."))
options.BoolVar(&__no_ordered_input__, "no-order", __no_ordered_input__,
options.Description("When several input files are provided, "+
"indicates that there is no order among them."))
@@ -116,6 +124,10 @@ func OptionSet(options *getoptions.GetOpt) {
// file has to be printed.
func CLIInputFormat() string {
switch {
case __input_fasta_format__:
return "fasta"
case __input_fastq_format__:
return "fastq"
case __input_ecopcr_format__:
return "ecopcr"
case __input_embl_format__:

View File

@@ -138,6 +138,10 @@ func CLIReadBioSequences(filenames ...string) (obiiter.IBioSequence, error) {
}
switch CLIInputFormat() {
case "fastq":
reader = obiformats.ReadFastqFromFile
case "fasta":
reader = obiformats.ReadFastaFromFile
case "ecopcr":
reader = obiformats.ReadEcoPCRFromFile
case "embl":

View File

@@ -6,6 +6,7 @@ import (
log "github.com/sirupsen/logrus"
"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiapat"
"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiformats/ncbitaxdump"
"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitax"
@@ -43,6 +44,11 @@ var _SaveRejected = ""
var _PairedMode = "forward"
var _approx_pattern = make([]string, 0)
var _pattern_error = 0
var _pattern_indel = false
var _pattern_only_forward = false
func TaxonomySelectionOptionSet(options *getoptions.GetOpt) {
options.StringVar(&_Taxdump, "taxdump", _Taxdump,
@@ -143,6 +149,23 @@ func SequenceSelectionOptionSet(options *getoptions.GetOpt) {
options.Description("If paired reads are passed to obibrep, that option determines how the conditions "+
"are applied to both reads."),
)
options.StringSliceVar(&_approx_pattern, "approx-pattern", 1, 1,
options.ArgName("PATTERN"),
options.Description("Regular expression pattern to be tested against the sequence itself. The pattern is case insensitive."))
options.IntVar(&_pattern_error, "pattern-error", _pattern_error,
options.Description("Maximum number of allowed error during pattern matching"),
)
options.BoolVar(&_pattern_indel, "allows-indels", _pattern_indel,
options.Description("Allows for indel during pattern matching"),
)
options.BoolVar(&_pattern_only_forward, "only-forward", _pattern_only_forward,
options.Description("Look for pattern only on forward strand"),
)
}
// OptionSet adds to the basic option set every options declared for
@@ -212,6 +235,18 @@ func CLISelectedNCBITaxDump() string {
return _Taxdump
}
func CLIPatternError() int {
return _pattern_error
}
func CLIPatternInDels() bool {
return _pattern_indel
}
func CLIPatternBothStrand() bool {
return !_pattern_only_forward
}
func CLILoadSelectedTaxonomy() *obitax.Taxonomy {
if CLISelectedNCBITaxDump() != "" {
if _Taxonomy == nil {
@@ -327,6 +362,30 @@ func CLISequencePatternPredicate() obiseq.SequencePredicate {
return nil
}
func CLISequenceAgrep() obiseq.SequencePredicate {
if len(_approx_pattern) > 0 {
p := obiapat.IsPatternMatchSequence(
_approx_pattern[0],
CLIPatternError(),
CLIPatternBothStrand(),
CLIPatternInDels(),
)
for _, pattern := range _approx_pattern[1:] {
p = p.And(obiapat.IsPatternMatchSequence(
pattern,
CLIPatternError(),
CLIPatternBothStrand(),
CLIPatternInDels(),
))
}
return p
}
return nil
}
func CLIDefinitionPatternPredicate() obiseq.SequencePredicate {
if len(_DefinitionPatterns) > 0 {
@@ -419,6 +478,7 @@ func CLISequenceSelectionPredicate() obiseq.SequencePredicate {
p = p.And(CLIIdListPredicate())
p = p.And(CLIHasAttibutePredicate())
p = p.And(CLIIsAttibuteMatchPredicate())
p = p.And(CLISequenceAgrep())
if _InvertMatch {
p = p.Not()