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https://github.com/metabarcoding/obitools4.git
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Add EMBL rope parsing support and improve sequence extraction
Introduce EmblChunkParserRope function to parse EMBL chunks directly from a rope without using Pack(). Add extractEmblSeq helper to scan sequence sections and handle U to T conversion. Update parser logic to use rope-based parsing when available, and fix feature table handling for WGS entries.
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@@ -161,6 +161,149 @@ func EmblChunkParser(withFeatureTable, UtoT bool) func(string, io.Reader) (obise
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return parser
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}
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// extractEmblSeq scans the sequence section of an EMBL record directly on the
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// rope. EMBL sequence lines start with 5 spaces followed by bases in groups of
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// 10, separated by spaces, with a position number at the end. The section ends
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// with "//".
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func (s *ropeScanner) extractEmblSeq(dest []byte, UtoT bool) []byte {
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// We use ReadLine and scan each line for bases (skip digits, spaces, newlines).
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for {
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line := s.ReadLine()
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if line == nil {
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break
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}
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if len(line) >= 2 && line[0] == '/' && line[1] == '/' {
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break
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}
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// Lines start with 5 spaces; bases follow separated by single spaces.
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// Digits at the end are the position counter — skip them.
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// Simplest: take every byte that is a letter.
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for _, b := range line {
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if b >= 'A' && b <= 'Z' {
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b += 'a' - 'A'
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}
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if UtoT && b == 'u' {
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b = 't'
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}
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if b >= 'a' && b <= 'z' {
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dest = append(dest, b)
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}
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}
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}
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return dest
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}
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// EmblChunkParserRope parses an EMBL chunk directly from a rope without Pack().
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func EmblChunkParserRope(source string, rope *PieceOfChunk, withFeatureTable, UtoT bool) (obiseq.BioSequenceSlice, error) {
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scanner := newRopeScanner(rope)
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sequences := obiseq.MakeBioSequenceSlice(100)[:0]
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var id string
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var scientificName string
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defBytes := make([]byte, 0, 256)
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featBytes := make([]byte, 0, 1024)
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var taxid int
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inSeq := false
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for {
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line := scanner.ReadLine()
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if line == nil {
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break
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}
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if inSeq {
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// Should not happen — extractEmblSeq consumed up to "//"
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inSeq = false
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continue
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}
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switch {
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case bytes.HasPrefix(line, []byte("ID ")):
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id = string(bytes.SplitN(line[5:], []byte(";"), 2)[0])
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case bytes.HasPrefix(line, []byte("OS ")):
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scientificName = string(bytes.TrimSpace(line[5:]))
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case bytes.HasPrefix(line, []byte("DE ")):
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if len(defBytes) > 0 {
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defBytes = append(defBytes, ' ')
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}
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defBytes = append(defBytes, bytes.TrimSpace(line[5:])...)
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case withFeatureTable && bytes.HasPrefix(line, []byte("FH ")):
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featBytes = append(featBytes, line...)
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case withFeatureTable && bytes.Equal(line, []byte("FH")):
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featBytes = append(featBytes, '\n')
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featBytes = append(featBytes, line...)
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case bytes.HasPrefix(line, []byte("FT ")):
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if withFeatureTable {
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featBytes = append(featBytes, '\n')
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featBytes = append(featBytes, line...)
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}
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if bytes.HasPrefix(line, []byte(`FT /db_xref="taxon:`)) {
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rest := line[37:]
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end := bytes.IndexByte(rest, '"')
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if end > 0 {
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taxid, _ = strconv.Atoi(string(rest[:end]))
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}
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}
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case bytes.HasPrefix(line, []byte(" ")):
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// First sequence line: extract all bases via extractEmblSeq,
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// which also consumes this line's remaining content.
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// But ReadLine already consumed this line — we need to process it
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// plus subsequent lines. Process this line inline then call helper.
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seqDest := make([]byte, 0, 4096)
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for _, b := range line {
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if b >= 'A' && b <= 'Z' {
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b += 'a' - 'A'
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}
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if UtoT && b == 'u' {
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b = 't'
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}
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if b >= 'a' && b <= 'z' {
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seqDest = append(seqDest, b)
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}
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}
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seqDest = scanner.extractEmblSeq(seqDest, UtoT)
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seq := obiseq.NewBioSequenceOwning(id, seqDest, string(defBytes))
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seq.SetSource(source)
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if withFeatureTable {
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seq.SetFeatures(featBytes)
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}
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annot := seq.Annotations()
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annot["scientific_name"] = scientificName
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annot["taxid"] = taxid
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sequences = append(sequences, seq)
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// Reset state
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id = ""
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scientificName = ""
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defBytes = defBytes[:0]
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featBytes = featBytes[:0]
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taxid = 1
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case bytes.Equal(line, []byte("//")):
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// record ended without SQ/sequence section (e.g. WGS entries)
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if id != "" {
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seq := obiseq.NewBioSequenceOwning(id, []byte{}, string(defBytes))
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seq.SetSource(source)
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if withFeatureTable {
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seq.SetFeatures(featBytes)
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}
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annot := seq.Annotations()
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annot["scientific_name"] = scientificName
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annot["taxid"] = taxid
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sequences = append(sequences, seq)
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}
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id = ""
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scientificName = ""
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defBytes = defBytes[:0]
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featBytes = featBytes[:0]
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taxid = 1
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}
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}
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return sequences, nil
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}
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func _ParseEmblFile(
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input ChannelFileChunk,
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out obiiter.IBioSequence,
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@@ -171,7 +314,14 @@ func _ParseEmblFile(
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for chunks := range input {
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order := chunks.Order
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sequences, err := parser(chunks.Source, chunks.Raw)
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var sequences obiseq.BioSequenceSlice
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var err error
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if chunks.Rope != nil {
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sequences, err = EmblChunkParserRope(chunks.Source, chunks.Rope, withFeatureTable, UtoT)
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} else {
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sequences, err = parser(chunks.Source, chunks.Raw)
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}
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if err != nil {
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log.Fatalf("%s : Cannot parse the embl file : %v", chunks.Source, err)
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@@ -196,7 +346,7 @@ func ReadEMBL(reader io.Reader, options ...WithOption) (obiiter.IBioSequence, er
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1024*1024*128,
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EndOfLastFlatFileEntry,
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"\nID ",
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true,
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false,
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)
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newIter := obiiter.MakeIBioSequence()
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