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Patch a bug for multiple amplicon per sequence.

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Former-commit-id: b252d2de8e1a85d65c2951aa1958ee038e35741d
This commit is contained in:
Eric Coissac
2023-03-31 15:10:25 +02:00
parent 84b3e4d097
commit 3f69fa41d6
4 changed files with 42 additions and 33 deletions
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34
pkg/obiapat/pcr.go
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@@ -201,7 +201,6 @@ func OptionBatchSize(size int) WithOption {
}
func _Pcr(seq ApatSequence,
sequence *obiseq.BioSequence,
opt Options) obiseq.BioSequenceSlice {
results := make(obiseq.BioSequenceSlice, 0, 10)
@@ -218,7 +217,7 @@ func _Pcr(seq ApatSequence,
length := seq.Len() - begin
if opt.pointer.maxLength > 0 {
length = forwardMatches[len(forwardMatches)-1][2] - begin + opt.MaxLength() + reverse.Len()
length = forwardMatches[len(forwardMatches)-1][1] - begin + opt.MaxLength() + reverse.Len()
}
if opt.Circular() {
@@ -254,26 +253,27 @@ func _Pcr(seq ApatSequence,
if length > 0 && // For when primers touch or overlap
(opt.MinLength() == 0 || length >= opt.MinLength()) &&
(opt.MaxLength() == 0 || length <= opt.MaxLength()) {
amplicon, _ := sequence.Subsequence(fm[1], rm[0], opt.pointer.circular)
amplicon, _ := seq.pointer.reference.Subsequence(fm[1], rm[0], opt.pointer.circular)
log.Debugf("seq length : %d capacity : %d",amplicon.Len(),cap(amplicon.Sequence()))
annot := amplicon.Annotations()
obiutils.MustFillMap(annot, sequence.Annotations())
obiutils.MustFillMap(annot, seq.pointer.reference.Annotations())
annot["forward_primer"] = forward.String()
match, _ := sequence.Subsequence(fm[0], fm[1], opt.pointer.circular)
match, _ := seq.pointer.reference.Subsequence(fm[0], fm[1], opt.pointer.circular)
annot["forward_match"] = match.String()
match.Recycle()
annot["forward_error"] = erri
annot["reverse_primer"] = reverse.String()
match, _ = sequence.Subsequence(rm[0], rm[1], opt.pointer.circular)
match, _ = seq.pointer.reference.Subsequence(rm[0], rm[1], opt.pointer.circular)
match = match.ReverseComplement(true)
annot["reverse_match"] = match.String()
match.Recycle()
annot["reverse_error"] = errj
annot["direction"] = "forward"
// log.Debugf("amplicon sequence capacity : %d", cap(amplicon.Sequence()))
@@ -287,13 +287,14 @@ func _Pcr(seq ApatSequence,
}
forwardMatches = reverse.FindAllIndex(seq, 0, -1)
if forwardMatches != nil {
begin := forwardMatches[0][0]
length := seq.Len() - begin
if opt.pointer.maxLength > 0 {
length = forwardMatches[len(forwardMatches)-1][2] - begin + opt.MaxLength() + reverse.Len()
length = forwardMatches[len(forwardMatches)-1][1] - begin + opt.MaxLength() + reverse.Len()
}
if opt.Circular() {
@@ -302,6 +303,7 @@ func _Pcr(seq ApatSequence,
}
reverseMatches := cfwd.FindAllIndex(seq, begin, length)
if reverseMatches != nil {
for _, fm := range forwardMatches {
@@ -329,14 +331,14 @@ func _Pcr(seq ApatSequence,
if length > 0 && // For when primers touch or overlap
(opt.MinLength() == 0 || length >= opt.MinLength()) &&
(opt.MaxLength() == 0 || length <= opt.MaxLength()) {
amplicon, _ := sequence.Subsequence(fm[1], rm[0], opt.pointer.circular)
amplicon, _ := seq.pointer.reference.Subsequence(fm[1], rm[0], opt.pointer.circular)
amplicon = amplicon.ReverseComplement(true)
annot := amplicon.Annotations()
obiutils.MustFillMap(annot, sequence.Annotations())
obiutils.MustFillMap(annot, seq.pointer.reference.Annotations())
annot["forward_primer"] = forward.String()
match, _ := sequence.Subsequence(rm[0], rm[1], opt.pointer.circular)
match, _ := seq.pointer.reference.Subsequence(rm[0], rm[1], opt.pointer.circular)
match.ReverseComplement(true)
annot["forward_match"] = match.String()
match.Recycle()
@@ -344,11 +346,13 @@ func _Pcr(seq ApatSequence,
annot["forward_error"] = errj
annot["reverse_primer"] = reverse.String()
match, _ = sequence.Subsequence(fm[0], fm[1], opt.pointer.circular)
match, _ = seq.pointer.reference.Subsequence(fm[0], fm[1], opt.pointer.circular)
annot["reverse_match"] = match.String()
match.Recycle()
annot["reverse_error"] = erri
annot["direction"] = "reverse"
results = append(results, amplicon)
// log.Debugf("amplicon sequence capacity : %d", cap(amplicon.Sequence()))
}
@@ -372,7 +376,7 @@ func PCRSim(sequence *obiseq.BioSequence, options ...WithOption) obiseq.BioSeque
seq, _ := MakeApatSequence(sequence, opt.Circular())
defer seq.Free()
results := _Pcr(seq, sequence, opt)
results := _Pcr(seq, opt)
return results
}
@@ -388,16 +392,16 @@ func _PCRSlice(sequences obiseq.BioSequenceSlice,
// if AllocatedApaSequences() == 0 {
// log.Panicln("Bizarre....")
// }
amplicons := _Pcr(seq, sequences[0], options)
amplicons := _Pcr(seq, options)
if len(amplicons) > 0 {
results = append(results, amplicons...)
}
log.Debugf("Number of sequences in the slice : %d",len(sequences))
for _, sequence := range sequences[1:] {
seq, _ = MakeApatSequence(sequence, options.Circular(), seq)
amplicons = _Pcr(seq, sequence, options)
amplicons = _Pcr(seq, options)
if len(amplicons) > 0 {
results = append(results, amplicons...)
}