Patch rev complement and first implementation of --auto in obicsv

Former-commit-id: f3020e81283b1073c4d1c2d2ff0887e3998e6764
This commit is contained in:
2023-11-07 09:37:07 +02:00
parent 6a6a6f6f2c
commit 61c30f9b6a
21 changed files with 270 additions and 107 deletions

View File

@@ -10,6 +10,7 @@ import (
"time"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiiter"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obioptions"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiutils"
log "github.com/sirupsen/logrus"
@@ -65,7 +66,7 @@ func CSVRecord(sequence *obiseq.BioSequence, opt Options) []string {
l := sequence.Len()
q := sequence.Qualities()
ascii := make([]byte, l)
quality_shift := opt.QualityShift()
quality_shift := obioptions.OutputQualityShift()
for j := 0; j < l; j++ {
ascii[j] = uint8(q[j]) + uint8(quality_shift)
}
@@ -130,6 +131,8 @@ func FormatCVSBatch(batch obiiter.BioSequenceBatch, opt Options) []byte {
func WriteCSV(iterator obiiter.IBioSequence,
file io.WriteCloser,
options ...WithOption) (obiiter.IBioSequence, error) {
var auto_slot obiutils.Set[string]
opt := MakeOptions(options)
file, _ = obiutils.CompressStream(file, opt.CompressedFile(), opt.CloseFile())
@@ -167,12 +170,6 @@ func WriteCSV(iterator obiiter.IBioSequence,
newIter.Done()
}
log.Debugln("Start of the CSV file writing")
go ff(iterator)
for i := 0; i < nwriters-1; i++ {
go ff(iterator.Split())
}
next_to_send := 0
received := make(map[int]FileChunck, 100)
@@ -203,6 +200,25 @@ func WriteCSV(iterator obiiter.IBioSequence,
}()
if opt.pointer.csv_auto {
if iterator.Next() {
batch := iterator.Get()
auto_slot = batch.Slice().AttributeKeys(true)
CSVKeys(auto_slot.Members())(opt)
chunkchan <- FileChunck{
FormatCVSBatch(batch, opt),
batch.Order(),
}
newIter.Push(batch)
}
}
log.Debugln("Start of the CSV file writing")
go ff(iterator)
for i := 0; i < nwriters-1; i++ {
go ff(iterator.Split())
}
return newIter, nil
}

View File

@@ -234,7 +234,7 @@ func ParseFastaChunk(source string, ch FastxChunk) *obiiter.BioSequenceBatch {
C = C + 'a' - 'A'
}
// Removing white space from the sequence
if (C >= 'a' && C <= 'z') || C == '-' || C == '.' {
if (C >= 'a' && C <= 'z') || C == '-' || C == '.' || C == '[' || C == ']' {
ch.Bytes[current] = C
current++
}

View File

@@ -329,7 +329,7 @@ func ReadFastq(reader io.Reader, options ...WithOption) (obiiter.IBioSequence, e
parser := func() {
defer out.Done()
for chk := range chkchan {
seqs := ParseFastqChunk(source, chk, byte(opt.QualityShift()))
seqs := ParseFastqChunk(source, chk, byte(obioptions.InputQualityShift()))
if seqs != nil {
out.Push(*seqs)
} else {

View File

@@ -15,6 +15,7 @@ import (
log "github.com/sirupsen/logrus"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiiter"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obioptions"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiutils"
)
@@ -91,7 +92,7 @@ func ReadFastSeqFromFile(filename string, options ...WithOption) (obiiter.IBioSe
name := C.CString(filename)
defer C.free(unsafe.Pointer(name))
pointer := C.open_fast_sek_file(name, C.int32_t(opt.QualityShift()))
pointer := C.open_fast_sek_file(name, C.int32_t(obioptions.InputQualityShift()))
var err error
err = nil
@@ -150,7 +151,7 @@ func ReadFastSeqFromStdin(options ...WithOption) obiiter.IBioSequence {
}(newIter)
go _FastseqReader(opt.Source(),
C.open_fast_sek_stdin(C.int32_t(opt.QualityShift())),
C.open_fast_sek_stdin(C.int32_t(obioptions.InputQualityShift())),
newIter, opt.BatchSize())
log.Debugln("Full file batch mode : ", opt.FullFileBatch())

View File

@@ -11,21 +11,16 @@ import (
log "github.com/sirupsen/logrus"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiiter"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obioptions"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiutils"
)
// The function FormatFastq takes a BioSequence object, a quality shift value, and a header formatter
// function as input, and returns a formatted string in FASTQ format.
func FormatFastq(seq *obiseq.BioSequence, quality_shift int, formater FormatHeader) string {
func FormatFastq(seq *obiseq.BioSequence, formater FormatHeader) string {
l := seq.Len()
q := seq.Qualities()
ascii := make([]byte, seq.Len())
for j := 0; j < l; j++ {
ascii[j] = uint8(q[j]) + uint8(quality_shift)
}
q := seq.QualitiesString()
info := ""
if formater != nil {
@@ -34,8 +29,8 @@ func FormatFastq(seq *obiseq.BioSequence, quality_shift int, formater FormatHead
return fmt.Sprintf("@%s %s\n%s\n+\n%s",
seq.Id(), info,
string(seq.Sequence()),
string(ascii),
seq.String(),
q,
)
}
@@ -44,7 +39,7 @@ func FormatFastqBatch(batch obiiter.BioSequenceBatch, quality_shift int,
var bs bytes.Buffer
for _, seq := range batch.Slice() {
if seq.Len() > 0 {
bs.WriteString(FormatFastq(seq, quality_shift, formater))
bs.WriteString(FormatFastq(seq, formater))
bs.WriteString("\n")
} else {
if skipEmpty {
@@ -81,7 +76,7 @@ func WriteFastq(iterator obiiter.IBioSequence,
chunkchan := make(chan FileChunck)
header_format := opt.FormatFastSeqHeader()
quality := opt.QualityShift()
quality := obioptions.OutputQualityShift()
newIter.Add(nwriters)

View File

@@ -12,7 +12,6 @@ type __options__ struct {
buffer_size int
batch_size int
full_file_batch bool
quality_shift int
parallel_workers int
closefile bool
appendfile bool
@@ -27,6 +26,7 @@ type __options__ struct {
csv_keys []string
csv_separator string
csv_navalue string
csv_auto bool
paired_filename string
source string
}
@@ -43,7 +43,6 @@ func MakeOptions(setters []WithOption) Options {
fastseq_header_writer: FormatFastSeqJsonHeader,
with_progress_bar: false,
buffer_size: 2,
quality_shift: 33,
parallel_workers: obioptions.CLIReadParallelWorkers(),
batch_size: obioptions.CLIBatchSize(),
full_file_batch: false,
@@ -60,6 +59,7 @@ func MakeOptions(setters []WithOption) Options {
csv_separator: ",",
csv_navalue: "NA",
csv_keys: make([]string, 0),
csv_auto: false,
paired_filename: "",
source: "",
}
@@ -73,10 +73,6 @@ func MakeOptions(setters []WithOption) Options {
return opt
}
func (opt Options) QualityShift() int {
return opt.pointer.quality_shift
}
func (opt Options) BatchSize() int {
return opt.pointer.batch_size
}
@@ -153,6 +149,10 @@ func (opt Options) CSVNAValue() string {
return opt.pointer.csv_navalue
}
func (opt Options) CSVAutoColumn() bool {
return opt.pointer.csv_auto
}
func (opt Options) HaveToSavePaired() bool {
return opt.pointer.paired_filename != ""
}
@@ -217,31 +217,6 @@ func OptionsNewFile() WithOption {
return f
}
// Allows to specify the ascii code corresponding to
// a quality of 0 in fastq encoded quality scores.
func OptionsQualityShift(shift int) WithOption {
f := WithOption(func(opt Options) {
opt.pointer.quality_shift = shift
})
return f
}
// Allows to specify a quality shift of 33, corresponding
// to a FastQ file qualities encoded following Sanger
// convention. This corresponds to Illumina produced FastQ
// files.
func OptionsQualitySanger() WithOption {
return OptionsQualityShift(33)
}
// Allows to specify a quality shift of 64, corresponding
// to a FastQ file qualities encoded following the Solexa
// convention.
func OptionsQualitySolexa() WithOption {
return OptionsQualityShift(64)
}
func OptionsFastSeqHeaderParser(parser obiseq.SeqAnnotator) WithOption {
f := WithOption(func(opt Options) {
opt.pointer.fastseq_header_parser = parser
@@ -403,3 +378,11 @@ func CSVNAValue(navalue string) WithOption {
return f
}
func CSVAutoColumn(auto bool) WithOption {
f := WithOption(func(opt Options) {
opt.pointer.csv_auto = auto
})
return f
}