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volume = 227,
year = 1985,
bdsk-url-1 = {http://www.ncbi.nlm.nih.gov/pubmed/2983426}}
@ARTICLE{Shehzad2012-pn,
title = "{Carnivore diet analysis based on next-generation sequencing:
Application to the leopard cat (Prionailurus bengalensis) in
Pakistan}",
author = "Shehzad, Wasim and Riaz, Tiayyba and Nawaz, Muhammad A and
Miquel, Christian and Poillot, Carole and Shah, Safdar A and
Pompanon, Francois and Coissac, Eric and Taberlet, Pierre",
journal = "Molecular ecology",
publisher = "Wiley Online Library",
volume = 21,
number = 8,
pages = "1951--1965",
year = 2012,
url = "https://onlinelibrary.wiley.com/doi/abs/10.1111/j.1365-294X.2011.05424.x",
issn = "0962-1083"
}
@ARTICLE{Riaz2011-gn,
title = "{ecoPrimers: inference of new DNA barcode markers from whole
genome sequence analysis}",
author = "Riaz, Tiayyba and Shehzad, Wasim and Viari, Alain and Pompanon,
Fran{\c c}ois and Taberlet, Pierre and Coissac, Eric",
abstract = "Using non-conventional markers, DNA metabarcoding allows
biodiversity assessment from complex substrates. In this article,
we present ecoPrimers, a software for identifying new barcode
markers and their associated PCR primers. ecoPrimers scans whole
genomes to find such markers without a priori knowledge.
ecoPrimers optimizes two quality indices measuring taxonomical
range and discrimination to select the most efficient markers
from a set of reference sequences, according to specific
experimental constraints such as marker length or specifically
targeted taxa. The key step of the algorithm is the
identification of conserved regions among reference sequences for
anchoring primers. We propose an efficient algorithm based on
data mining, that allows the analysis of huge sets of sequences.
We evaluate the efficiency of ecoPrimers by running it on three
different sequence sets: mitochondrial, chloroplast and bacterial
genomes. Identified barcode markers correspond either to barcode
regions already in use for plants or animals, or to new potential
barcodes. Results from empirical experiments carried out on a
promising new barcode for analyzing vertebrate diversity fully
agree with expectations based on bioinformatics analysis. These
tests demonstrate the efficiency of ecoPrimers for inferring new
barcodes fitting with diverse experimental contexts. ecoPrimers
is available as an open source project at:
http://www.grenoble.prabi.fr/trac/ecoPrimers.",
journal = "Nucleic acids research",
volume = 39,
number = 21,
pages = "e145",
month = nov,
year = 2011,
url = "http://dx.doi.org/10.1093/nar/gkr732",
language = "en",
issn = "0305-1048, 1362-4962",
pmid = "21930509",
doi = "10.1093/nar/gkr732",
pmc = "PMC3241669"
}
@ARTICLE{Seguritan2001-tg,
title = "{FastGroup: a program to dereplicate libraries of 16S rDNA
sequences}",
author = "Seguritan, V and Rohwer, F",
abstract = "BACKGROUND: Ribosomal 16S DNA sequences are an essential tool for
identifying and classifying microbes. High-throughput DNA
sequencing now makes it economically possible to produce very
large datasets of 16S rDNA sequences in short time periods,
necessitating new computer tools for analyses. Here we describe
FastGroup, a Java program designed to dereplicate libraries of
16S rDNA sequences. By dereplication we mean to: 1) compare all
the sequences in a data set to each other, 2) group similar
sequences together, and 3) output a representative sequence from
each group. In this way, duplicate sequences are removed from a
library. RESULTS: FastGroup was tested using a library of
single-pass, bacterial 16S rDNA sequences cloned from
coral-associated bacteria. We found that the optimal strategy for
dereplicating these sequences was to: 1) trim ambiguous bases
from the 5' end of the sequences and all sequence 3' of the
conserved Bact517 site, 2) match the sequences from the 3' end,
and 3) group sequences > or =97\% identical to each other.
CONCLUSIONS: The FastGroup program simplifies the dereplication
of 16S rDNA sequence libraries and prepares the raw sequences for
subsequent analyses.",
journal = "BMC bioinformatics",
volume = 2,
pages = "9",
month = oct,
year = 2001,
url = "http://dx.doi.org/10.1186/1471-2105-2-9",
language = "en",
issn = "1471-2105",
pmid = "11707150",
doi = "10.1186/1471-2105-2-9",
pmc = "PMC59723"
}