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168
pkg/obiformats/fastseq_write_fastq.go
Normal file
168
pkg/obiformats/fastseq_write_fastq.go
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package obiformats
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import (
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"bytes"
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"fmt"
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"io"
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"log"
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"os"
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"time"
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"git.metabarcoding.org/lecasofts/go/oa2/pkg/obiseq"
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)
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func FormatFastq(seq obiseq.BioSequence, quality_shift int, formater FormatHeader) string {
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l := seq.Length()
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q := seq.Qualities()
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ascii := make([]byte, seq.Length())
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for j := 0; j < l; j++ {
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ascii[j] = uint8(q[j]) + uint8(quality_shift)
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}
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info := ""
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if formater != nil {
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info = formater(seq)
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}
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return fmt.Sprintf("@%s %s %s\n%s\n+\n%s",
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seq.Id(), info,
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seq.Definition(),
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string(seq.Sequence()),
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string(ascii),
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)
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}
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func FormatFastqBatch(batch obiseq.BioSequenceBatch, quality_shift int,
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formater FormatHeader) []byte {
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var bs bytes.Buffer
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for _, seq := range batch.Slice() {
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bs.WriteString(FormatFastq(seq, quality_shift, formater))
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bs.WriteString("\n")
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}
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return bs.Bytes()
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}
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func WriteFastq(iterator obiseq.IBioSequence, file io.Writer, options ...WithOption) error {
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opt := MakeOptions(options)
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header_format := opt.FormatFastSeqHeader()
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quality := opt.QualityShift()
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for iterator.Next() {
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seq := iterator.Get()
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fmt.Fprintln(file, FormatFastq(seq, quality, header_format))
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}
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return nil
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}
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func WriteFastqToFile(iterator obiseq.IBioSequence,
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filename string,
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options ...WithOption) error {
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file, err := os.Create(filename)
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if err != nil {
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log.Fatalf("open file error: %v", err)
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return err
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}
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return WriteFastq(iterator, file, options...)
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}
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func WriteFastqToStdout(iterator obiseq.IBioSequence, options ...WithOption) error {
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return WriteFastq(iterator, os.Stdout, options...)
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}
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type FileChunck struct {
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text []byte
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order int
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}
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func WriteFastqBatch(iterator obiseq.IBioSequenceBatch, file io.Writer, options ...WithOption) (obiseq.IBioSequenceBatch, error) {
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buffsize := iterator.BufferSize()
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new_iter := obiseq.MakeIBioSequenceBatch(buffsize)
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opt := MakeOptions(options)
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nwriters := 4
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chunkchan := make(chan FileChunck)
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header_format := opt.FormatFastSeqHeader()
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quality := opt.QualityShift()
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new_iter.Add(nwriters)
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go func() {
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new_iter.Wait()
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for len(chunkchan) > 0 {
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time.Sleep(time.Millisecond)
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}
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close(chunkchan)
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for len(new_iter.Channel()) > 0 {
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time.Sleep(time.Millisecond)
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}
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close(new_iter.Channel())
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}()
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ff := func(iterator obiseq.IBioSequenceBatch) {
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for iterator.Next() {
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batch := iterator.Get()
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chunkchan <- FileChunck{
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FormatFastqBatch(batch, quality, header_format),
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batch.Order(),
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}
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new_iter.Channel() <- batch
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}
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new_iter.Done()
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}
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log.Println("Start of the fastq file reading")
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for i := 0; i < nwriters; i++ {
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go ff(iterator.Split())
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}
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next_to_send := 0
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received := make(map[int]FileChunck, 100)
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go func() {
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for chunk := range chunkchan {
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if chunk.order == next_to_send {
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file.Write(chunk.text)
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next_to_send++
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chunk, ok := received[next_to_send]
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for ok {
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file.Write(chunk.text)
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delete(received, next_to_send)
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next_to_send++
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chunk, ok = received[next_to_send]
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}
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} else {
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received[chunk.order] = chunk
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}
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}
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}()
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return new_iter, nil
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}
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func WriteFastqBatchToStdout(iterator obiseq.IBioSequenceBatch, options ...WithOption) (obiseq.IBioSequenceBatch, error) {
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return WriteFastqBatch(iterator, os.Stdout, options...)
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}
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func WriteFastqBatchToFile(iterator obiseq.IBioSequenceBatch,
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filename string,
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options ...WithOption) (obiseq.IBioSequenceBatch, error) {
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file, err := os.Create(filename)
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if err != nil {
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log.Fatalf("open file error: %v", err)
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return obiseq.NilIBioSequenceBatch, err
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}
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return WriteFastqBatch(iterator, file, options...)
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}
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