4 Annexes
4.0.1 Sequence attributes
4.0.1.1 Reserved sequence attributes
4.0.1.1.1 ali_dir
4.0.1.1.1.2 Set by the obipairing tool
The alignment generated by obipairing is a 3’-end gap free algorithm.
Two cases can occur when aligning the forward and reverse reads. If the
barcode is long enough, both the reads overlap only on their 3’ ends. In
such case, the alignment direction ali_dir is set to left. If the
barcode is shorter than the read length, the paired reads overlap by
their 5’ ends, and the complete barcode is sequenced by both the reads.
In that later case, ali_dir is set to right.
4.0.1.1.3 count : the number of sequence occurrences
4.0.1.1.4 merged_*
4.0.1.1.4.2 Set by the obiuniq tool
The -m option of the obiuniq tools allows for keeping track of the
distribution of the values stored in given attribute of interest. Often
this option is used to summarise distribution of a sequence variant
accross samples when obiuniq is run after running obimultiplex. The
actual name of the attribute depends on the name of the monitored
attribute. If -m option is used with the attribute sample, then this
attribute names merged_sample.