Patch RPS12 detection

This commit is contained in:
Eric Coissac
2025-05-25 10:31:30 +02:00
parent c5b92799b1
commit 9205fd1ed1
4 changed files with 16 additions and 5 deletions

View File

@ -31,6 +31,10 @@ else
QUERY="$1"
fi
shift
GENOME_LENGHT="$2"
if (( $# > 1 )) ; then
TEMP=$2
else
@ -45,7 +49,6 @@ AnnotFile="$CDS_DATA_DIR/sp_chlorodb/Annot.lst"
ModelsDir="$CDS_DATA_DIR/sp_chlorodb/models"
SEQLEN=$(seqlength "${QUERY}")
SEQUENCE=$(readfirstfastaseq "${QUERY}")
pushTmpDir ORG.RPS12
@ -78,7 +81,10 @@ blastx \
{PREV_CDS = $2;}
(BEST_EVAL > ($11 + 0.0)) {BEST_EVAL = ($11 + 0.0)}
' > "rps12_locate.hsps"
' \
| $AwkCmd -v glength=${GENOME_LENGHT} \
'!($7 + 0 > glength + 0 && $8 + 0 > glength + 0)' \
> "rps12_locate.hsps"
#

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@ -9,6 +9,7 @@
# go_cds.sh <FASTAFILE> [DBROOT]
#
# - <FASTAFILE> : The fasta file containing the genome to annotate
# - <GENOME_LENGHT> : The length of the genome to annotate
# - [DBROOT] : optionnal argument allowing to specify database directory
#
# Results are printed to the standard output
@ -28,12 +29,14 @@ else
Threads=$ANNOT_MAXCPU
fi
needarg 1
needarg 2
Fasta=$1; shift
needfile "$Fasta"
GenomeLength=$1; shift
# Genome names is set from the base
# name of the genome file without its extension
Genome=$(basename ${Fasta%.*})
@ -92,7 +95,7 @@ fi
if [[ "$cdsdetection_pass2" == "yes" ]] ; then
loginfo "running pass2:rps12 exonerate of $Genome on $DbRoot"
$PROG_DIR/do_rps12.sh $Fasta $temp
$PROG_DIR/do_rps12.sh $Fasta $GenomeLength $temp
fi
#

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@ -34,6 +34,7 @@ function convert(p1,p2) {
r = $0
while (length(s) > 0) {
match(s,/[0-9]+\.\.[0-9]+/)
if (RLENGTH+0 < 0) break
range = substr(s,RSTART,RLENGTH)
s = substr(s,RSTART+RLENGTH+1)
match(range,/^[0-9]+/)

View File

@ -441,6 +441,7 @@ do
shift
done
loginfo "Locus tag prefix provided: $tagprefix"
loginfo "Locus tag numbered from..: $locusshift"
loginfo "NCBI taxid provided......: $taxid"
@ -572,7 +573,7 @@ pushTmpDir ORG.organnot
cdsdetection_pass1=$cdsdetection_pass1 \
cdsdetection_pass2=$cdsdetection_pass2 \
cdsdetection_pass3=$cdsdetection_pass3 \
${PROG_DIR}/detectors/cds/bin/go_cds.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
${PROG_DIR}/detectors/cds/bin/go_cds.sh "${RESULTS}.norm.fasta" "$sl" >> "${RESULTS}.annot"
loginfo "Done."
fi