add a -l option to set a minimum length on the contig to annotate
Former-commit-id: a16feefc3a4d02c1b053e8ece340a65e966b58af Former-commit-id: 072e73cbf437c0308f287ac1b558bad269e85f94
This commit is contained in:
388
org-annotate.sh
388
org-annotate.sh
@ -25,6 +25,7 @@ irdetection="yes"
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organism="no"
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types="chloro"
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partial=0
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minlength=0
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function usage {
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echo "Usage:" ;
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@ -56,6 +57,9 @@ function usage {
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echo
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echo ' -p | --partial'
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echo ' Indicates that the genome sequence is partial and therefore in several contigs'
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echo
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echo ' -l | --min-length'
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echo ' Indicates for partial mode the minimum length of contig to annotate'
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exit $2
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}
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@ -67,7 +71,7 @@ function fastaIterator() {
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}
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# options may be followed by one colon to indicate they have a required argument
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if ! options=$(getopt -o t:o:icrmhp -l ncbi-taxid:,organism,no-ir-detection,chloroplast,nuclear-rdna,mitochondrion,partial,help -- "$@")
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if ! options=$(getopt -o t:o:icrmhpl: -l ncbi-taxid:,organism,no-ir-detection,chloroplast,nuclear-rdna,mitochondrion,partial,min-length:,help -- "$@")
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then
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# something went wrong, getopt will put out an error message for us
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usage $0 1
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@ -85,6 +89,7 @@ do
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-r|--nuclear-rdna) types="nucrdna" ;;
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-m|--mitochondrion) types="mito" ;;
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-p|--partial) partial="1" ;;
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-l|--min-length) minlength="$2" ; shift ;;
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-h|--help) usage $0 0;;
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(--) shift; break;;
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(-*) echo "$0: error - unrecognized option $1" 1>&2; exit 1;;
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@ -97,6 +102,7 @@ loginfo "Annotating mode.....: $types"
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loginfo "IR detection mode...: $irdetection"
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loginfo "Organism............: $organism"
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loginfo "Partial mode........: $partial"
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loginfo "Minimum length......: $minlength"
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#############################
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@ -124,209 +130,213 @@ pushTmpDir ORG.organnot
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unset IFS
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if [[ ! -z "${sequence}" ]] ; then
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echo "${sequence}" > toannotate.fasta
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sl=$(seqlength "toannotate.fasta")
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seqid=$($AwkCmd '(NR==1) {print substr($1,2,1000)}' toannotate.fasta)
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if (( sl >= minlength )) ; then
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case "$types" in
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chloro)
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loginfo "Annotating a plant chloroplast genome..."
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if [[ "$irdetection" == "yes" ]] && (( partial == 0 )) ; then
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seqid=$($AwkCmd '(NR==1) {print substr($1,2,1000)}' toannotate.fasta)
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loginfo "Normalizing the structure of the Chloroplast sequence..."
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loginfo " LSC + IRB + SSC + IRA"
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${PROG_DIR}/detectors/normalize/bin/go_normalize.sh toannotate.fasta > "${RESULTS}.norm.fasta"
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case "$types" in
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chloro)
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loginfo "Annotating a plant chloroplast genome..."
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if [[ "$irdetection" == "yes" ]] && (( partial == 0 )) ; then
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loginfo "Normalizing the structure of the Chloroplast sequence..."
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loginfo " LSC + IRB + SSC + IRA"
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${PROG_DIR}/detectors/normalize/bin/go_normalize.sh toannotate.fasta > "${RESULTS}.norm.fasta"
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loginfo "Done."
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loginfo "Annotating the Inverted repeats and Single copies (LSC and SSC)..."
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${PROG_DIR}/detectors/ir/bin/go_ir.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
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loginfo "Done."
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else
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loginfo "No normalization of the structure of the Chloroplast sequence..."
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cat toannotate.fasta > "${RESULTS}.norm.fasta"
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rm -f "${RESULTS}.annot"
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touch "${RESULTS}.annot"
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fi
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loginfo "Annotating the tRNA..."
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${PROG_DIR}/detectors/trna/bin/go_trna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
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loginfo "Done."
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loginfo "Annotating the Inverted repeats and Single copies (LSC and SSC)..."
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${PROG_DIR}/detectors/ir/bin/go_ir.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
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loginfo "Annotating the rRNA genes..."
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${PROG_DIR}/detectors/rrna/bin/go_rrna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
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loginfo "Done."
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loginfo "Annotating the CDS..."
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tcsh -f ${PROG_DIR}/detectors/cds/bin/go_cds.csh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
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loginfo "Done."
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else
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loginfo "No normalization of the structure of the Chloroplast sequence..."
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cat toannotate.fasta > "${RESULTS}.norm.fasta"
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rm -f "${RESULTS}.annot"
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touch "${RESULTS}.annot"
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fi
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loginfo "Annotating the tRNA..."
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${PROG_DIR}/detectors/trna/bin/go_trna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
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loginfo "Done."
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loginfo "Annotating the rRNA genes..."
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${PROG_DIR}/detectors/rrna/bin/go_rrna.sh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
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loginfo "Done."
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loginfo "Annotating the CDS..."
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tcsh -f ${PROG_DIR}/detectors/cds/bin/go_cds.csh "${RESULTS}.norm.fasta" >> "${RESULTS}.annot"
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loginfo "Done."
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if (( partial == 0 )) ; then
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topology="circular"
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defline="plastid, complete genome"
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else
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topology="linear"
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defline="plastid, partial sequence"
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fi
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;;
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nucrdna)
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loginfo "Annotating a plant rDNA cistron..."
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loginfo "Normalizing the structure of the cistron sequence..."
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${PROG_DIR}/detectors/normalizerdna/bin/go_normalizerdna.sh toannotate.fasta > "${RESULTS}.norm.fasta"
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loginfo "Done."
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loginfo "Annotating the rRNA genes..."
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${PROG_DIR}/detectors/nucrrna/bin/go_nucrrna.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
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loginfo "Done."
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topology="linear"
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defline="18S rRNA gene, ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene"
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;;
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mito)
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loginfo "Annotating an animal mitochondrial genome..."
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logerror "Not yet implemented"
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if (( partial == 0 )) ; then
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topology="circular"
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defline="mitochondrion, complete genome"
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else
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topology="linear"
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defline="mitochondrion, partial sequence"
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fi
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exit 1
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;;
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*)
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usage $0 1;;
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esac
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if [[ "${organism}" == "no" ]]; then
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organism="{organism}"
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else
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organism="$(echo ${organism} | tr '_' ' ')"
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fi
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if (( partial == 0 )) ; then
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topology="circular"
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defline="plastid, complete genome"
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else
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topology="linear"
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defline="plastid, partial sequence"
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fi
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;;
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nucrdna)
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loginfo "Annotating a plant rDNA cistron..."
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loginfo "Normalizing the structure of the cistron sequence..."
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${PROG_DIR}/detectors/normalizerdna/bin/go_normalizerdna.sh toannotate.fasta > "${RESULTS}.norm.fasta"
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loginfo "Done."
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loginfo "Annotating the rRNA genes..."
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${PROG_DIR}/detectors/nucrrna/bin/go_nucrrna.sh "${RESULTS}.norm.fasta" > "${RESULTS}.annot"
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loginfo "Done."
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sl=$(seqlength "${RESULTS}.norm.fasta")
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topology="linear"
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defline="18S rRNA gene, ITS1, 5.8S rRNA gene, ITS2 and 28S rRNA gene"
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;;
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mito)
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loginfo "Annotating an animal mitochondrial genome..."
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logerror "Not yet implemented"
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loginfo "Printing minimal header..."
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echo "ID ${seqid}; ${seqid}; ${topology}; genomic DNA; XXX; XXX; ${sl} BP."
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echo "XX"
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echo "AC ${seqid};"
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echo "DE ${organism} ${defline}."
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echo "XX"
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loginfo "Done."
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loginfo "Printing annotations header..."
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echo "FH Key Location/Qualifiers"
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loginfo "Done."
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loginfo "Printing the source feature"
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echo "FT source 1..${sl}"
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if [[ "${organism}" != "{organism}" ]] ; then
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echo "FT /organism=\"${organism}\""
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fi
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case "${types}" in
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chloro)
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echo "FT /organelle=\"plastid:chloroplast\""
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;;
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mito)
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echo "FT /organelle=\"mitochondrion\""
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;;
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if (( partial == 0 )) ; then
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topology="circular"
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defline="mitochondrion, complete genome"
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else
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topology="linear"
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defline="mitochondrion, partial sequence"
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fi
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exit 1
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;;
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*)
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loginfo "Nuclear sequence"
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;;
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usage $0 1;;
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esac
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echo "FT /mol_type=\"genomic DNA\""
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if [[ "${taxid}" != "no" ]] ; then
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echo "FT /db_xref=\"taxon:${taxid}\""
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if [[ "${organism}" == "no" ]]; then
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organism="{organism}"
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else
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organism="$(echo ${organism} | tr '_' ' ')"
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fi
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# echo "FT /country=\"Poland: Bialowieza Forest\""
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loginfo "Done."
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loginfo "Ordering annotations..."
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$AwkCmd '(entry && /^.....(misc|repeat|rRNA|tRNA|gene|source)/) { \
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print pos,entry } \
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/^.....(misc|repeat|rRNA|tRNA|gene|source)/ { \
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match($3,"[0-9][0-9]*"); \
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pos=substr($3,RSTART,RLENGTH)*1000 + 1; \
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entry=$0; \
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next} \
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{ entry=entry "@" $0} \
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END {print pos,entry}' "${RESULTS}.annot" | \
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sort -nk1 |\
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$AwkCmd '{ \
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match($0,"^[0-9]* ");\
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line=substr($0,RLENGTH+1);\
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gsub("@","\n",line); \
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print line}'
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loginfo "Done."
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loginfo "Closing annotations table..."
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echo "XX"
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loginfo "Done."
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loginfo "Computing statistics on nucleotide usage..."
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$AwkCmd '! /^>/ { \
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seq=toupper($0); \
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gsub(" ","",seq); \
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lseq=length(seq); \
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for (i=0; i < lseq; i++) { \
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freq[substr(seq,i,1)]++}\
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} \
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END { \
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other=0; \
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for (i in freq) { \
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if (i!="A" && i!="C" && i!="G" && i!="T") {\
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other+=freq[i] \
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} \
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}; \
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print "SQ Sequence "\
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(freq["A"]+freq["C"]+freq["G"]+freq["T"]+other) \
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" BP; "\
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freq["A"]" A; "\
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freq["C"]" C; "\
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freq["G"]" G; "\
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freq["T"]" T; "\
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other" other;" \
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}' "${RESULTS}.norm.fasta"
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loginfo "Done."
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loginfo "Reformating sequences..."
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lines=$(wc -l "${RESULTS}.norm.fasta" | $AwkCmd '{print $1}')
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sl=$(seqlength "${RESULTS}.norm.fasta")
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loginfo "Sequence length $(seqlength ${RESULTS}.norm.fasta)"
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loginfo "lines $lines"
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formatfasta "${RESULTS}.norm.fasta" | \
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$AwkCmd -v lines=$lines ' \
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! /^>/ { \
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seq=tolower($0); \
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gsub(" ","",seq); \
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printf(" ") ;\
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for (i=0; i < 6; i++) { \
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f=substr(seq,i * 10 + 1, 10); \
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pos+=length(f); \
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f = f substr(" ",1,10-length(f)); \
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printf("%s ",f) \
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}; \
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printf(" %6d\n",pos) \
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}'
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loginfo "Done."
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loginfo "Printing minimal header..."
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echo "ID ${seqid}; ${seqid}; ${topology}; genomic DNA; XXX; XXX; ${sl} BP."
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echo "XX"
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echo "AC ${seqid};"
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echo "DE ${organism} ${defline}."
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echo "XX"
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loginfo "Done."
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loginfo "Closing sequence part..."
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echo "//"
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loginfo "Done."
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fi
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loginfo "Printing annotations header..."
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echo "FH Key Location/Qualifiers"
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loginfo "Done."
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loginfo "Printing the source feature"
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echo "FT source 1..${sl}"
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if [[ "${organism}" != "{organism}" ]] ; then
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echo "FT /organism=\"${organism}\""
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fi
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case "${types}" in
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chloro)
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echo "FT /organelle=\"plastid:chloroplast\""
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;;
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mito)
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echo "FT /organelle=\"mitochondrion\""
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;;
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*)
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loginfo "Nuclear sequence"
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;;
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esac
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echo "FT /mol_type=\"genomic DNA\""
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if [[ "${taxid}" != "no" ]] ; then
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echo "FT /db_xref=\"taxon:${taxid}\""
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fi
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# echo "FT /country=\"Poland: Bialowieza Forest\""
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loginfo "Done."
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loginfo "Ordering annotations..."
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$AwkCmd '(entry && /^.....(misc|repeat|rRNA|tRNA|gene|source)/) { \
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print pos,entry } \
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/^.....(misc|repeat|rRNA|tRNA|gene|source)/ { \
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match($3,"[0-9][0-9]*"); \
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pos=substr($3,RSTART,RLENGTH)*1000 + 1; \
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entry=$0; \
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next} \
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{ entry=entry "@" $0} \
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END {print pos,entry}' "${RESULTS}.annot" | \
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sort -nk1 |\
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$AwkCmd '{ \
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match($0,"^[0-9]* ");\
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line=substr($0,RLENGTH+1);\
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gsub("@","\n",line); \
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print line}'
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loginfo "Done."
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loginfo "Closing annotations table..."
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echo "XX"
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loginfo "Done."
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loginfo "Computing statistics on nucleotide usage..."
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$AwkCmd '! /^>/ { \
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seq=toupper($0); \
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gsub(" ","",seq); \
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lseq=length(seq); \
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for (i=0; i < lseq; i++) { \
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freq[substr(seq,i,1)]++}\
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} \
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END { \
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other=0; \
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for (i in freq) { \
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if (i!="A" && i!="C" && i!="G" && i!="T") {\
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other+=freq[i] \
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} \
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}; \
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print "SQ Sequence "\
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(freq["A"]+freq["C"]+freq["G"]+freq["T"]+other) \
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" BP; "\
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freq["A"]" A; "\
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freq["C"]" C; "\
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freq["G"]" G; "\
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freq["T"]" T; "\
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other" other;" \
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}' "${RESULTS}.norm.fasta"
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loginfo "Done."
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loginfo "Reformating sequences..."
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lines=$(wc -l "${RESULTS}.norm.fasta" | $AwkCmd '{print $1}')
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loginfo "Sequence length $(seqlength ${RESULTS}.norm.fasta)"
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loginfo "lines $lines"
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formatfasta "${RESULTS}.norm.fasta" | \
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$AwkCmd -v lines=$lines ' \
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! /^>/ { \
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seq=tolower($0); \
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gsub(" ","",seq); \
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printf(" ") ;\
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for (i=0; i < 6; i++) { \
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f=substr(seq,i * 10 + 1, 10); \
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pos+=length(f); \
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f = f substr(" ",1,10-length(f)); \
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printf("%s ",f) \
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}; \
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printf(" %6d\n",pos) \
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}'
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loginfo "Done."
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loginfo "Closing sequence part..."
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echo "//"
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loginfo "Done."
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fi # End of the minimum length condition
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fi # End of not empty sequence condition
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IFS=$'\f'
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done # End of the loop over the sequences
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