obitools/ecoprimers
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Added command line options for:

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a. 'salt method' like "-m 1" or "-m 2" here 1 is for SANTALUCIA and 2 is for OWCZARZY
b. 'salt concentration' "-a 0.01" valid range is from 0.01 to 0.3. if not specified then we use default 0.05

git-svn-id: https://www.grenoble.prabi.fr/svn/LECASofts/ecoPrimers/trunk@224 60f365c0-8329-0410-b2a4-ec073aeeaa1d
This commit is contained in:
Eric Coissac
2009-07-14 22:23:18 +00:00
parent e79738e170
commit 50c26e81b9
3 changed files with 27 additions and 11 deletions
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28
src/ecoprimer.c
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@ -157,6 +157,8 @@ void initoptions(poptions_t options)
options->no_multi_match=FALSE;
options->pnparm = NULL;
strcpy(options->taxonrank, DEFAULTTAXONRANK); /*taxon level for results, species by default*/
options->saltmethod = SALT_METHOD_SANTALUCIA;
options->salt = DEF_SALT;
}
void printapair(int32_t index,ppair_t pair, poptions_t options)
@ -540,11 +542,10 @@ int main(int argc, char **argv)
//printf ("\nGETREVCODE: A=%d, C=%d, G=%d, T=%d\n", GETREVCODE(t[0]), GETREVCODE('C'), GETREVCODE('G'), GETREVCODE('T'));
//return 0;
CNNParams nnparams;
nparam_InitParams(&nnparams, DEF_CONC_PRIMERS,DEF_CONC_SEQUENCES,DEF_SALT,SALT_METHOD_SANTALUCIA);
initoptions(&options);
while ((carg = getopt(argc, argv, "hfvcUDSd:l:L:e:i:r:R:q:3:s:x:t:O:")) != -1) {
while ((carg = getopt(argc, argv, "hfvcUDSd:l:L:e:i:r:R:q:3:s:x:t:O:m:a:")) != -1) {
switch (carg) {
/* ---------------------------- */
@ -674,7 +675,19 @@ int main(int argc, char **argv)
sscanf(optarg,"%d",&(options.primer_length));
break;
/* -------------------- */
/* --------------------------------- */
case 'm': /* set salt method */
/* --------------------------------- */
sscanf(optarg,"%d",&(options.saltmethod));
break;
/* --------------------------------- */
case 'a': /* set salt */
/* --------------------------------- */
sscanf(optarg,"%f",&(options.salt));
break;
/* -------------------- */
case 'c': /* sequences are circular */
/* --------------------------------- */
options.circular = 1;
@ -687,6 +700,15 @@ int main(int argc, char **argv)
}
options.pnparm = &nnparams;
if (options.saltmethod != 2) //if not SALT_METHOD_OWCZARZY
options.saltmethod = SALT_METHOD_SANTALUCIA; //then force SALT_METHOD_SANTALUCIA
if (options.salt < 0.01 || options.salt > 0.3) //if salt value out of literature values
options.salt = DEF_SALT; //set to default
nparam_InitParams(&nnparams, DEF_CONC_PRIMERS,DEF_CONC_SEQUENCES,options.salt,options.saltmethod);
fprintf(stderr,"Reading taxonomy database ...");
taxonomy = read_taxonomy(options.prefix,0);
fprintf(stderr,"Ok\n");

2
src/libecoprimer/ecoprimer.h
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@ -278,6 +278,8 @@ typedef struct {
int32_t outsamples;
int32_t intaxa;
int32_t outtaxa;
int saltmethod;
float salt;
PNNParams pnparm;
} options_t, *poptions_t;

8
src/libthermo/nnparams.c
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@ -89,14 +89,6 @@ float nparam_CalcTM(float entropy,float enthalpy)
return tm;
}
/* PURPOSE: Initialize nearest neighbor parameters.
*
* PARAMETERS:
* none
*
* RETURN VALUE:
* void
*/
void nparam_InitParams(PNNParams nparm, float c1, float c2, float kp, int sm)
{