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Author SHA1 Message Date
6d445fe3ad switch to version 3.0.1b5 2021-03-22 09:41:01 +13:00
824deb7e21 new command: obi rm: deletes any view (for now the user deleting a view
accepts that there will be missing information when running obi history
if other views came from the deleted view)
2021-03-18 09:17:06 +13:00
d579bb2749 switch to version 3.0.1b4 2021-03-16 17:40:58 +13:00
10e5ebdbc0 ngsfilter: fixed critical bug where barcodes shorter than the forward
primer would be missed
2021-03-16 15:09:28 +13:00
8833110490 import: fixed the import of tabular files with no header 2021-03-16 09:15:48 +13:00
bd38449f2d switch to version 3.0.1b3 2021-03-15 16:50:17 +13:00
904823c827 uniq: now OK to use -m option even if only one unique key in information
to merge (e.g. one sample)
2021-03-15 16:48:22 +13:00
af68a1024c Switch to version 3.0.1b2 2021-03-15 16:26:43 +13:00
425fe25bd2 Made the OBITools3 more 'empty file friendly' 2021-03-15 16:25:41 +13:00
d48aed38d4 switch to version 3.0.1b1 2021-03-11 17:11:23 +13:00
5e32f8523e Merge branch 'wsl_version' 2021-03-11 16:47:59 +13:00
cba78111c9 obi test: fixed bug introduced in previous version 2021-03-11 11:36:52 +13:00
9 changed files with 145 additions and 83 deletions

View File

@ -205,19 +205,25 @@ def run(config):
if type(entries) == list:
forward = entries[0]
reverse = entries[1]
aligner = Kmer_similarity(forward, \
view2=reverse, \
kmer_size=config['alignpairedend']['kmersize'], \
reversed_column=None)
if len(forward) == 0 or len(reverse) == 0:
aligner = None
else:
aligner = Kmer_similarity(forward, \
view2=reverse, \
kmer_size=config['alignpairedend']['kmersize'], \
reversed_column=None)
else:
aligner = Kmer_similarity(entries, \
column2=entries[REVERSE_SEQUENCE_COLUMN], \
qual_column2=entries[REVERSE_QUALITY_COLUMN], \
kmer_size=config['alignpairedend']['kmersize'], \
reversed_column=entries[b'reversed']) # column created by the ngsfilter tool
if len(entries) == 0:
aligner = None
else:
aligner = Kmer_similarity(entries, \
column2=entries[REVERSE_SEQUENCE_COLUMN], \
qual_column2=entries[REVERSE_QUALITY_COLUMN], \
kmer_size=config['alignpairedend']['kmersize'], \
reversed_column=entries[b'reversed']) # column created by the ngsfilter tool
ba = alignmentIterator(entries, aligner)
i = 0
for ali in ba:
@ -251,7 +257,7 @@ def run(config):
pb(i, force=True)
print("", file=sys.stderr)
if kmer_ali :
if kmer_ali and aligner is not None:
aligner.free()
# Save command config in View and DMS comments

View File

@ -322,7 +322,7 @@ cdef tuple annotate(sequences, infos, no_tags, verbose=False):
sequences[0] = sequences[0][directmatch[1][2]:]
else:
sequences[1] = sequences[1][directmatch[1][2]:]
sequences[0][REVERSE_SEQUENCE_COLUMN] = sequences[1].seq # used by alignpairedend tool
sequences[0][REVERSE_SEQUENCE_COLUMN] = sequences[1].seq # used by alignpairedend tool
sequences[0][REVERSE_QUALITY_COLUMN] = sequences[1].quality # used by alignpairedend tool
if directmatch[0].forward:
@ -369,7 +369,7 @@ cdef tuple annotate(sequences, infos, no_tags, verbose=False):
sequences[0] = sequences[0][:r[1]]
else:
sequences[1] = sequences[1][:r[1]]
sequences[0][REVERSE_SEQUENCE_COLUMN] = sequences[1].seq # used by alignpairedend tool
sequences[0][REVERSE_SEQUENCE_COLUMN] = sequences[1].seq # used by alignpairedend tool
sequences[0][REVERSE_QUALITY_COLUMN] = sequences[1].quality # used by alignpairedend tool
# do the same on the other seq
if first_match_first_seq:
@ -394,7 +394,7 @@ cdef tuple annotate(sequences, infos, no_tags, verbose=False):
seq_to_match = sequences[0]
reversematch = []
# Compute begin
begin=directmatch[1][2]+1 # end of match + 1 on the same sequence
#begin=directmatch[1][2]+1 # end of match + 1 on the same sequence -- No, already cut out forward primer
# Try reverse matching on the other sequence:
new_seq = True
pattern = 0
@ -408,7 +408,7 @@ cdef tuple annotate(sequences, infos, no_tags, verbose=False):
primer=p
# Saving original primer as 4th member of the tuple to serve as correct key in infos dict even if it might have been reversed complemented
# (3rd member already used by directmatch)
reversematch.append((primer, primer(seq_to_match, same_sequence=not new_seq, pattern=pattern, begin=begin), None, p))
reversematch.append((primer, primer(seq_to_match, same_sequence=not new_seq, pattern=pattern, begin=0), None, p))
new_seq = False
pattern+=1
# Choose match closer to the end of the sequence
@ -645,6 +645,7 @@ def run(config):
g = 0
u = 0
i = 0
no_tags = config['ngsfilter']['notags']
try:
for i in range(entries_len):

View File

@ -0,0 +1,44 @@
#cython: language_level=3
from obitools3.uri.decode import open_uri
from obitools3.apps.config import logger
from obitools3.dms import DMS
from obitools3.apps.optiongroups import addMinimalInputOption
from obitools3.dms.view.view cimport View
import os
__title__="Delete a view"
def addOptions(parser):
addMinimalInputOption(parser)
def run(config):
DMS.obi_atexit()
logger("info", "obi rm")
# Open the input
input = open_uri(config['obi']['inputURI'])
if input is None:
raise Exception("Could not read input")
# Check that it's a view
if isinstance(input[1], View) :
view = input[1]
else:
raise NotImplementedError()
# Get the path to the view file to remove
path = input[0].full_path # dms path
path+=b"/VIEWS/"
path+=view.name
path+=b".obiview"
# Close the view and the DMS
view.close()
input[0].close(force=True)
# Rm
os.remove(path)

View File

@ -354,8 +354,8 @@ cdef uniq_sequences(View_NUC_SEQS view, View_NUC_SEQS o_view, ProgressBar pb, di
key = mergedKeys[k]
merged_col_name = mergedKeys_m[k]
if merged_infos[merged_col_name]['nb_elts'] == 1:
raise Exception("Can't merge information from a tag with only one element (e.g. one sample ; don't use -m option)")
# if merged_infos[merged_col_name]['nb_elts'] == 1:
# raise Exception("Can't merge information from a tag with only one element (e.g. one sample ; don't use -m option)")
if merged_col_name in view:
i_col = view[merged_col_name]

View File

@ -600,7 +600,8 @@ cdef class View(OBIWrapper) :
if element is not None:
if element.comments[b"input_dms_name"] is not None :
for i in range(len(element.comments[b"input_dms_name"])) :
if element.comments[b"input_dms_name"][i] == element.dms.name and b"/" not in element.comments[b"input_view_name"][i]: # Same DMS and not a special element like a taxonomy
if b"/" not in element.comments[b"input_view_name"][i] and element.comments[b"input_view_name"][i] in element.dms \
and element.comments[b"input_dms_name"][i] == element.dms.name : # Same DMS and not a special element like a taxonomy and view was not deleted
top_level.append(element.dms[element.comments[b"input_view_name"][i]])
else:
top_level.append(None)

View File

@ -8,7 +8,7 @@ Created on feb 20th 2018
import types
from obitools3.utils cimport __etag__
from obitools3.utils cimport str2bytes
def tabIterator(lineiterator,
bint header = False,
@ -75,7 +75,7 @@ def tabIterator(lineiterator,
continue
else:
# TODO ??? default column names? like R?
keys = [i for i in range(len(line.split(sep)))]
keys = [str2bytes(str(i)) for i in range(len(line.split(sep)))]
while skipped < skip :
line = next(iterator)

View File

@ -53,7 +53,11 @@ def entryIteratorFactory(lineiterator,
i = iterator
first=next(i)
try:
first=next(i)
except StopIteration:
first=""
pass
format=b"tabular"

View File

@ -1,5 +1,5 @@
major = 3
minor = 0
serial= '0b43'
serial= '1b5'
version ="%d.%d.%s" % (major,minor,serial)

View File

@ -229,6 +229,8 @@ int obi_clean(const char* dms_name,
return -1;
}
seq_count = (i_view->infos)->line_count;
// Open the sequence column
if (strcmp((i_view->infos)->view_type, VIEW_TYPE_NUC_SEQS) == 0)
iseq_column = obi_view_get_column(i_view, NUC_SEQUENCE_COLUMN);
@ -245,7 +247,7 @@ int obi_clean(const char* dms_name,
}
// Open the sample column if there is one
if ((strcmp(sample_column_name, "") == 0) || (sample_column_name == NULL))
if ((strcmp(sample_column_name, "") == 0) || (sample_column_name == NULL) || (seq_count == 0))
{
fprintf(stderr, "Info: No sample information provided, assuming one sample.\n");
sample_column = obi_view_get_column(i_view, COUNT_COLUMN);
@ -340,66 +342,67 @@ int obi_clean(const char* dms_name,
return -1;
}
// Build kmer tables
ktable = hash_seq_column(i_view, iseq_column, 0);
if (ktable == NULL)
if (seq_count > 0)
{
obi_set_errno(OBI_CLEAN_ERROR);
obidebug(1, "\nError building kmer tables before aligning");
return -1;
}
// Build kmer tables
ktable = hash_seq_column(i_view, iseq_column, 0);
if (ktable == NULL)
{
obi_set_errno(OBI_CLEAN_ERROR);
obidebug(1, "\nError building kmer tables before aligning");
return -1;
}
seq_count = (i_view->infos)->line_count;
// Allocate arrays for sample counts otherwise reading in mapped files takes longer
complete_sample_count_array = (int*) malloc(seq_count * sample_count * sizeof(int));
if (complete_sample_count_array == NULL)
{
obi_set_errno(OBI_MALLOC_ERROR);
obidebug(1, "\nError allocating memory for the array of sample counts, size: %lld", seq_count * sample_count * sizeof(int));
return -1;
}
for (samp=0; samp < sample_count; samp++)
{
for (k=0; k<seq_count; k++)
complete_sample_count_array[k+(samp*seq_count)] = obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, k, samp);
}
// Allocate arrays for blobs otherwise reading in mapped files takes longer
blob_array = (Obi_blob_p*) malloc(seq_count * sizeof(Obi_blob_p));
if (blob_array == NULL)
{
obi_set_errno(OBI_MALLOC_ERROR);
obidebug(1, "\nError allocating memory for the array of blobs");
return -1;
}
for (k=0; k<seq_count; k++)
{
blob_array[k] = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, k, 0);
}
// Allocate alignment result array (byte at 0 if not aligned yet,
// 1 if sequence at index has a similarity above the threshold with the current sequence,
// 2 if sequence at index has a similarity below the threshold with the current sequence)
alignment_result_array = (byte_t*) calloc(seq_count, sizeof(byte_t));
if (alignment_result_array == NULL)
{
obi_set_errno(OBI_MALLOC_ERROR);
obidebug(1, "\nError allocating memory for alignment result array");
return -1;
}
// Initialize all sequences to singletons or NA if no sequences in that sample
for (k=0; k<seq_count; k++)
{
// Allocate arrays for sample counts otherwise reading in mapped files takes longer
complete_sample_count_array = (int*) malloc(seq_count * sample_count * sizeof(int));
if (complete_sample_count_array == NULL)
{
obi_set_errno(OBI_MALLOC_ERROR);
obidebug(1, "\nError allocating memory for the array of sample counts, size: %lld", seq_count * sample_count * sizeof(int));
return -1;
}
for (samp=0; samp < sample_count; samp++)
{
if (obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, k, samp) != OBIInt_NA) // Only initialize samples where there are some sequences
for (k=0; k<seq_count; k++)
complete_sample_count_array[k+(samp*seq_count)] = obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, k, samp);
}
// Allocate arrays for blobs otherwise reading in mapped files takes longer
blob_array = (Obi_blob_p*) malloc(seq_count * sizeof(Obi_blob_p));
if (blob_array == NULL)
{
obi_set_errno(OBI_MALLOC_ERROR);
obidebug(1, "\nError allocating memory for the array of blobs");
return -1;
}
for (k=0; k<seq_count; k++)
{
blob_array[k] = obi_get_blob_with_elt_idx_and_col_p_in_view(i_view, iseq_column, k, 0);
}
// Allocate alignment result array (byte at 0 if not aligned yet,
// 1 if sequence at index has a similarity above the threshold with the current sequence,
// 2 if sequence at index has a similarity below the threshold with the current sequence)
alignment_result_array = (byte_t*) calloc(seq_count, sizeof(byte_t));
if (alignment_result_array == NULL)
{
obi_set_errno(OBI_MALLOC_ERROR);
obidebug(1, "\nError allocating memory for alignment result array");
return -1;
}
// Initialize all sequences to singletons or NA if no sequences in that sample
for (k=0; k<seq_count; k++)
{
for (samp=0; samp < sample_count; samp++)
{
if (obi_set_char_with_elt_idx_and_col_p_in_view(o_view, status_column, k, samp, 's') < 0)
if (obi_get_int_with_elt_idx_and_col_p_in_view(i_view, sample_column, k, samp) != OBIInt_NA) // Only initialize samples where there are some sequences
{
obidebug(1, "\nError initializing all sequences to singletons");
return -1;
if (obi_set_char_with_elt_idx_and_col_p_in_view(o_view, status_column, k, samp, 's') < 0)
{
obidebug(1, "\nError initializing all sequences to singletons");
return -1;
}
}
}
}
@ -551,17 +554,20 @@ int obi_clean(const char* dms_name,
}
}
free_kmer_tables(ktable, seq_count);
free(complete_sample_count_array);
free(blob_array);
free(alignment_result_array);
if (seq_count > 0)
{
free_kmer_tables(ktable, seq_count);
free(complete_sample_count_array);
free(blob_array);
free(alignment_result_array);
}
fprintf(stderr, "\n");
if (stop)
return -1;
if (heads_only)
if (heads_only && (seq_count > 0))
{
line_selection = malloc((((o_view->infos)->line_count) + 1) * sizeof(index_t));
if (line_selection == NULL)
@ -635,7 +641,7 @@ int obi_clean(const char* dms_name,
}
// Flag the end of the line selection
if (heads_only)
if (heads_only && (seq_count > 0))
line_selection[l] = -1;
// Create new view with line selection if heads only