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Update Wolf tutorial with the OBITools3

Celine Mercier
2021-04-28 00:52:07 +02:00
parent 72303f4f2a
commit 77604f635b
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Wolf-tutorial-with-the-OBITools3.md

@ -55,11 +55,11 @@ And unzip it:
We can check the average alignment score (corresponding approximately to the similarity between the sequences in the overlap): We can check the average alignment score (corresponding approximately to the similarity between the sequences in the overlap):
obi stats -a score_norm wolf/aligned_reads obi stats -a score_norm wolf/aligned_reads
### 3. Remove unaligned sequence records ### 3. Remove sequence records with a low overlap alignment score:
obi grep -a mode:alignment wolf/aligned_reads wolf/good_sequences obi grep -p "sequence['score_norm'] > 0.8" wolf/aligned_reads wolf/good_sequences
### 4. Assign each sequence record to the corresponding sample/marker combination ### 4. Assign each sequence record to the corresponding sample/marker combination