mirror of
https://github.com/metabarcoding/obitools4.git
synced 2025-06-29 16:20:46 +00:00
Code refactoring
This commit is contained in:
@ -169,8 +169,8 @@ func BuildQualityConsensus(seqA, seqB obiseq.BioSequence, path []int,
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qm = qA
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}
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if qB == qA {
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nuc := __four_bits_base_code__[sA[i]&31] | __four_bits_base_code__[sB[i]&31]
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consensus = append(consensus, __four_bits_base_decode__[nuc])
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nuc := _FourBitsBaseCode[sA[i]&31] | _FourBitsBaseCode[sB[i]&31]
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consensus = append(consensus, _FourBitsBaseDecode[nuc])
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}
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q := qA + qB
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@ -74,8 +74,8 @@ func _MatchScoreRatio(a, b byte) (float64, float64) {
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func _InitNucPartMatch() {
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for i, a := range __four_bits_base_code__ {
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for j, b := range __four_bits_base_code__ {
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for i, a := range _FourBitsBaseCode {
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for j, b := range _FourBitsBaseCode {
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_NucPartMatch[i][j] = _MatchRatio(a, b)
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}
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}
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@ -4,7 +4,7 @@ import (
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"git.metabarcoding.org/lecasofts/go/obitools/pkg/obiseq"
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)
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var __four_bits_base_code__ = []byte{0b0000,
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var _FourBitsBaseCode = []byte{0b0000,
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// IUPAC nucleotide code Base
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0b0001, // A Adenine
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0b1110, // B C or G or T
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@ -38,7 +38,7 @@ var __four_bits_base_code__ = []byte{0b0000,
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0b0000,
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0b0000}
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var __four_bits_base_decode__ = []byte{
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var _FourBitsBaseDecode = []byte{
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// 0b0000 0b0001 0b0010 0b0011
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'.', 'a', 'c', 'm',
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// 0b0100 0b0101 0b0110 0b0111
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@ -49,6 +49,14 @@ var __four_bits_base_decode__ = []byte{
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'k', 'd', 'b', 'n',
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}
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// Encode4bits encodes each nucleotide of a sequence into a binary
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// code where the four low weigth bit of a byte correspond respectively
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// to the four nucleotides A, C, G, T. Simple bases A, C, G, T are therefore
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// represented by a code with only a single bit on, when anbiguous symboles
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// like R, D or N have the bits corresponding to each nucleotide represented
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// by the ambiguity set to 1.
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// A byte slice can be provided (buffer) to preveent allocation of a new
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// memory chunk by th function.
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func Encode4bits(seq obiseq.BioSequence, buffer []byte) []byte {
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length := seq.Length()
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rawseq := seq.Sequence()
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@ -65,7 +73,7 @@ func Encode4bits(seq obiseq.BioSequence, buffer []byte) []byte {
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if nuc == '.' || nuc == '-' {
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code = 0
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} else {
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code = __four_bits_base_code__[nuc&31]
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code = _FourBitsBaseCode[nuc&31]
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}
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buffer = append(buffer, code)
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}
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@ -51,17 +51,17 @@ func __get_matrix_from__(matrix *[]int, lenA, a, b int) (int, int, int) {
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}
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func __pairing_score_pe_align__(baseA, qualA, baseB, qualB byte) int {
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part_match := __nuc_part_match__[baseA&31][baseB&31]
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part_match := _NucPartMatch[baseA&31][baseB&31]
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// log.Printf("id : %f A : %s %d B : %s %d\n", part_match, string(baseA), qualA, string(baseB), qualB)
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switch {
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case part_match == 1:
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// log.Printf("match\n")
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return __nuc_score_part_match_match__[qualA][qualB]
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return _NucScorePartMatchMatch[qualA][qualB]
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case part_match == 0:
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return __nuc_score_part_match_mismatch__[qualA][qualB]
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return _NucScorePartMatchMismatch[qualA][qualB]
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default:
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return int(part_match*float64(__nuc_score_part_match_match__[qualA][qualB]) +
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(1-part_match)*float64(__nuc_score_part_match_mismatch__[qualA][qualB]) + 0.5)
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return int(part_match*float64(_NucScorePartMatchMatch[qualA][qualB]) +
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(1-part_match)*float64(_NucScorePartMatchMismatch[qualA][qualB]) + 0.5)
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}
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}
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@ -73,7 +73,7 @@ func __fill_matrix_pe_left_align__(seqA, qualA, seqB, qualB []byte, gap int,
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// The actual gap score is the gap score times the mismatch between
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// two bases with a score of 40
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gap = gap * __nuc_score_part_match_mismatch__[40][40]
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gap = gap * _NucScorePartMatchMismatch[40][40]
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needed := (la + 1) * (lb + 1)
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@ -144,7 +144,7 @@ func __fill_matrix_pe_right_align__(seqA, qualA, seqB, qualB []byte, gap int,
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// The actual gap score is the gap score times the mismatch between
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// two bases with a score of 40
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gap = gap * __nuc_score_part_match_mismatch__[40][40]
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gap = gap * _NucScorePartMatchMismatch[40][40]
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needed := (la + 1) * (lb + 1)
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@ -215,9 +215,9 @@ func __fill_matrix_pe_right_align__(seqA, qualA, seqB, qualB []byte, gap int,
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func PELeftAlign(seqA, seqB obiseq.BioSequence, gap int, arena PEAlignArena) (int, []int) {
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if !__initialized_dna_score__ {
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if !_InitializedDnaScore {
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log.Println("Initializing the DNA Scoring matrix")
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InitDNAScoreMatrix()
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_InitDNAScoreMatrix()
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}
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if arena.pointer == nil {
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@ -229,7 +229,7 @@ func PELeftAlign(seqA, seqB obiseq.BioSequence, gap int, arena PEAlignArena) (in
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&arena.pointer.score_matrix,
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&arena.pointer.path_matrix)
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arena.pointer.path = __backtracking__(arena.pointer.path_matrix,
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arena.pointer.path = _Backtracking(arena.pointer.path_matrix,
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seqA.Length(), seqB.Length(),
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&arena.pointer.path)
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@ -238,9 +238,9 @@ func PELeftAlign(seqA, seqB obiseq.BioSequence, gap int, arena PEAlignArena) (in
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func PERightAlign(seqA, seqB obiseq.BioSequence, gap int, arena PEAlignArena) (int, []int) {
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if !__initialized_dna_score__ {
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if !_InitializedDnaScore {
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log.Println("Initializing the DNA Scoring matrix")
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InitDNAScoreMatrix()
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_InitDNAScoreMatrix()
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}
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if arena.pointer == nil {
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@ -252,7 +252,7 @@ func PERightAlign(seqA, seqB obiseq.BioSequence, gap int, arena PEAlignArena) (i
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&arena.pointer.score_matrix,
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&arena.pointer.path_matrix)
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arena.pointer.path = __backtracking__(arena.pointer.path_matrix,
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arena.pointer.path = _Backtracking(arena.pointer.path_matrix,
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seqA.Length(), seqB.Length(),
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&arena.pointer.path)
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@ -269,9 +269,9 @@ func PEAlign(seqA, seqB obiseq.BioSequence,
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var raw_seqB, qual_seqB []byte
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var extra5, extra3 int
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if !__initialized_dna_score__ {
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if !_InitializedDnaScore {
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log.Println("Initializing the DNA Scoring matrix")
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InitDNAScoreMatrix()
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_InitDNAScoreMatrix()
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}
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index := obikmer.Index4mer(seqA,
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@ -323,7 +323,7 @@ func PEAlign(seqA, seqB obiseq.BioSequence,
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&arena.pointer.path_matrix)
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}
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arena.pointer.path = __backtracking__(arena.pointer.path_matrix,
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arena.pointer.path = _Backtracking(arena.pointer.path_matrix,
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len(raw_seqA), len(raw_seqB),
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&arena.pointer.path)
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@ -349,7 +349,7 @@ func PEAlign(seqA, seqB obiseq.BioSequence,
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score = 0
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for i, qualA := range qual_seqA {
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qualB := qual_seqB[i]
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score += __nuc_score_part_match_match__[qualA][qualB]
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score += _NucScorePartMatchMatch[qualA][qualB]
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}
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arena.pointer.path = arena.pointer.path[:0]
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arena.pointer.path = append(arena.pointer.path, 0, part_len)
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