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modified:   pkg/obitools/obitagpcr/pcrtag.go
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Eric Coissac
2024-10-29 16:53:10 +01:00
parent fefc360f80
commit 422f11cceb
1 changed files with 23 additions and 0 deletions
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pkg/obitools/obitagpcr/pcrtag.go
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@ -12,6 +12,29 @@ import (
"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obipairing"
)
// IPCRTagPESequencesBatch performs paired-end sequence demultiplexing and tagging.
// It takes an iterator of paired-end biosequences, and various parameters to control
// the demultiplexing and tagging process. It returns a new iterator with the
// demultiplexed and tagged sequences.
//
// The function first checks if the input iterator contains paired-end sequences.
// It then creates a new iterator to hold the processed sequences, and launches
// a number of worker goroutines to process the input sequences in parallel.
//
// For each sequence pair, the function attempts to assemble the paired-end
// sequences into a consensus sequence, and then extracts the barcodes and
// other metadata from the consensus. The extracted information is then
// added as annotations to the original sequence pair.
//
// If the CLI option to reorient the sequences is enabled, the function will
// reverse-complement the sequences as needed to ensure the forward and reverse
// tags are correctly oriented.
//
// If the CLI option to conserve unassigned sequences is disabled, the function
// will filter out any sequences that could not be demultiplexed.
//
// If the CLI option to save unassigned sequences is enabled, the function will
// write those sequences to a separate file.
func IPCRTagPESequencesBatch(iterator obiiter.IBioSequence,
gap, scale float64, delta, minOverlap int,
minIdentity float64, fastAlign, fastScoreRel,