Patch a bug in obitagpcr

.gitignore

@@ -118,3 +118,4 @@ doc/book/wolf_data/Release-253/ncbitaxo/readme.txt
 doc/book/results/toto.tasta
 sample/.DS_Store
 GO
+template.16S

pkg/obioptions/version.go

@@ -7,7 +7,7 @@ import (
 // TODO: The version number is extracted from git. This induces that the version
 // corresponds to the last commit, and not the one when the file will be
 // commited
-var _Commit = "fc75974"
+var _Commit = "4b65bfc"
 var _Version = "Release 4.3.0"
 
 // Version returns the version of the obitools package.

pkg/obiseq/biosequence.go

@@ -179,6 +179,9 @@ func (s *BioSequence) Copy() *BioSequence {
 	newSeq.sequence = CopySlice(s.sequence)
 	newSeq.qualities = CopySlice(s.qualities)
 	newSeq.feature = CopySlice(s.feature)
+	if s.revcomp != nil {
+		newSeq.revcomp = s.revcomp.Copy()
+	}
 
 	if len(s.annotations) > 0 {
 		s.annot_lock.Lock()

pkg/obiseq/revcomp.go

@@ -55,8 +55,7 @@ func (sequence *BioSequence) ReverseComplement(inplace bool) *BioSequence {
 
 	if !inplace {
 		original = sequence
-		sequence.revcomp = sequence.Copy()
-		sequence = sequence.revcomp
+		sequence = sequence.Copy()
 		sequence.revcomp = original
 	}
 

pkg/obitools/obitagpcr/pcrtag.go

@@ -134,8 +134,6 @@ func IPCRTagPESequencesBatch(iterator obiiter.IBioSequence,
 					banot["experiment"] = experiment
 
 					if CLIReorientate() && direction == "reverse" {
-						B.ReverseComplement(true)
-						A.ReverseComplement(true)
 						B.PairTo(A)
 						batch.Slice()[i] = B
 					}