Optimisation de la construction des binaires

Modification du fichier de workflow de release pour compiler uniquement les outils obitools lors de la construction des binaires pour chaque plateforme (Linux AMD64, Linux ARM64, macOS AMD64, macOS ARM64, Windows AMD64). Cela permet d'optimiser le processus de build en ne générant que les binaires nécessaires.

.github/workflows/release.yml

@@ -0,0 +1,176 @@
+name: Create Release on Tag
+
+on:
+  push:
+    tags:
+      - "Release_*"
+
+permissions:
+  contents: write
+
+jobs:
+  # First run tests
+  test:
+    runs-on: ubuntu-latest
+    steps:
+      - name: Setup Go
+        uses: actions/setup-go@v5
+        with:
+          go-version: "1.23"
+      - name: Checkout obitools4 project
+        uses: actions/checkout@v4
+      - name: Run tests
+        run: make githubtests
+
+  # Then create release only if tests pass
+  create-release:
+    needs: test
+    runs-on: ubuntu-latest
+    steps:
+      - name: Checkout code
+        uses: actions/checkout@v4
+        with:
+          fetch-depth: 0
+
+      - name: Setup Go
+        uses: actions/setup-go@v5
+        with:
+          go-version: "1.23"
+
+      - name: Extract version from tag
+        id: get_version
+        run: |
+          TAG=${GITHUB_REF#refs/tags/Release_}
+          echo "version=$TAG" >> $GITHUB_OUTPUT
+          echo "tag_name=Release_$TAG" >> $GITHUB_OUTPUT
+
+      - name: Build binaries for multiple platforms
+        env:
+          VERSION: ${{ steps.get_version.outputs.version }}
+        run: |
+          mkdir -p release
+
+          # Build for Linux AMD64
+          echo "Building for Linux AMD64..."
+          GOOS=linux GOARCH=amd64 make obitools
+          cd build
+          for binary in *; do
+            tar -czf ../release/${binary}_${VERSION}_linux_amd64.tar.gz ${binary}
+          done
+          cd ..
+          rm -rf build
+          
+
+          # Build for Linux ARM64
+          echo "Building for Linux ARM64..."
+          GOOS=linux GOARCH=arm64 make obitools
+          cd build
+          for binary in *; do
+            tar -czf ../release/${binary}_${VERSION}_linux_arm64.tar.gz ${binary}
+          done
+          cd ..
+          rm -rf build
+          
+
+          # Build for macOS AMD64 (Intel)
+          echo "Building for macOS AMD64..."
+          GOOS=darwin GOARCH=amd64 make obitools
+          cd build
+          for binary in *; do
+            tar -czf ../release/${binary}_${VERSION}_darwin_amd64.tar.gz ${binary}
+          done
+          cd ..
+          rm -rf build
+          
+
+          # Build for macOS ARM64 (Apple Silicon)
+          echo "Building for macOS ARM64..."
+          GOOS=darwin GOARCH=arm64 make obitools
+          cd build
+          for binary in *; do
+            tar -czf ../release/${binary}_${VERSION}_darwin_arm64.tar.gz ${binary}
+          done
+          cd ..
+          rm -rf build
+          
+
+          # Build for Windows AMD64
+          echo "Building for Windows AMD64..."
+          GOOS=windows GOARCH=amd64 make obitools
+          cd build
+          for binary in *; do
+            # Windows binaries have .exe extension
+            if [ -f "${binary}.exe" ]; then
+              zip ../release/${binary}_${VERSION}_windows_amd64.zip ${binary}.exe
+            else
+              zip ../release/${binary}_${VERSION}_windows_amd64.zip ${binary}
+            fi
+          done
+          cd ..
+
+          echo "Built archives:"
+          ls -lh release/
+
+      - name: Generate Release Notes
+        id: release_notes
+        env:
+          VERSION: ${{ steps.get_version.outputs.version }}
+        run: |
+          PREV_TAG=$(git describe --tags --abbrev=0 HEAD^ 2>/dev/null || echo "")
+
+          echo "# OBITools4 Release ${VERSION}" > release_notes.md
+          echo "" >> release_notes.md
+
+          if [ -n "$PREV_TAG" ]; then
+            echo "## Changes since ${PREV_TAG}" >> release_notes.md
+            echo "" >> release_notes.md
+            git log ${PREV_TAG}..HEAD --pretty=format:"- %s" >> release_notes.md
+          else
+            echo "## Changes" >> release_notes.md
+            echo "" >> release_notes.md
+            git log --pretty=format:"- %s" -n 20 >> release_notes.md
+          fi
+
+          echo "" >> release_notes.md
+          echo "" >> release_notes.md
+          echo "## Installation" >> release_notes.md
+          echo "" >> release_notes.md
+          echo "Download the appropriate binary for your system and extract it:" >> release_notes.md
+          echo "" >> release_notes.md
+          echo "### Linux (AMD64)" >> release_notes.md
+          echo '```bash' >> release_notes.md
+          echo "tar -xzf <tool>_${VERSION}_linux_amd64.tar.gz" >> release_notes.md
+          echo '```' >> release_notes.md
+          echo "" >> release_notes.md
+          echo "### Linux (ARM64)" >> release_notes.md
+          echo '```bash' >> release_notes.md
+          echo "tar -xzf <tool>_${VERSION}_linux_arm64.tar.gz" >> release_notes.md
+          echo '```' >> release_notes.md
+          echo "" >> release_notes.md
+          echo "### macOS (Intel)" >> release_notes.md
+          echo '```bash' >> release_notes.md
+          echo "tar -xzf <tool>_${VERSION}_darwin_amd64.tar.gz" >> release_notes.md
+          echo '```' >> release_notes.md
+          echo "" >> release_notes.md
+          echo "### macOS (Apple Silicon)" >> release_notes.md
+          echo '```bash' >> release_notes.md
+          echo "tar -xzf <tool>_${VERSION}_darwin_arm64.tar.gz" >> release_notes.md
+          echo '```' >> release_notes.md
+          echo "" >> release_notes.md
+          echo "### Windows (AMD64)" >> release_notes.md
+          echo '```powershell' >> release_notes.md
+          echo "Expand-Archive <tool>_${VERSION}_windows_amd64.zip" >> release_notes.md
+          echo '```' >> release_notes.md
+          echo "" >> release_notes.md
+          echo "Available tools: Replace \`<tool>\` with one of the obitools commands." >> release_notes.md
+
+      - name: Create GitHub Release
+        uses: softprops/action-gh-release@v1
+        with:
+          name: Release ${{ steps.get_version.outputs.version }}
+          body_path: release_notes.md
+          files: release/*
+          draft: false
+          prerelease: false
+        env:
+          GITHUB_TOKEN: ${{ secrets.GITHUB_TOKEN }}

.gitignore

@@ -16,12 +16,18 @@
 **/*.tgz
 **/*.yaml
 **/*.csv
+xx
 
 .rhistory
 /.vscode
 /build
+/bugs
 
 /ncbitaxo
 
 !/obitests/**
 !/sample/**
+LLM/** 
+*_files
+
+entropy.html

Makefile

@@ -2,8 +2,9 @@
 #export GOBIN=$(GOPATH)/bin
 #export PATH=$(GOBIN):$(shell echo $${PATH})
 
+GOFLAGS=
 GOCMD=go
-GOBUILD=$(GOCMD) build # -compiler gccgo -gccgoflags -O3
+GOBUILD=$(GOCMD) build $(GOFLAGS)
 GOGENERATE=$(GOCMD) generate
 GOCLEAN=$(GOCMD) clean
 GOTEST=$(GOCMD) test
@@ -16,6 +17,12 @@ PACKAGES_SRC:= $(wildcard pkg/*/*.go pkg/*/*/*.go)
 PACKAGE_DIRS:=$(sort $(patsubst %/,%,$(dir $(PACKAGES_SRC))))
 PACKAGES:=$(notdir $(PACKAGE_DIRS))
 
+GITHOOK_SRC_DIR=git-hooks
+GITHOOKS_SRC:=$(wildcard $(GITHOOK_SRC_DIR)/*)
+
+GITHOOK_DIR=.git/hooks
+GITHOOKS:=$(patsubst $(GITHOOK_SRC_DIR)/%,$(GITHOOK_DIR)/%,$(GITHOOKS_SRC))
+
 OBITOOLS_SRC:= $(wildcard cmd/obitools/*/*.go)
 OBITOOLS_DIRS:=$(sort $(patsubst %/,%,$(dir $(OBITOOLS_SRC))))
 OBITOOLS:=$(notdir $(OBITOOLS_DIRS))
@@ -53,34 +60,31 @@ endif
 
 OUTPUT:=$(shell mktemp)
 
-all: obitools
+all: install-githook obitools
+
+obitools: $(patsubst %,$(OBITOOLS_PREFIX)%,$(OBITOOLS))
+
+install-githook: $(GITHOOKS)
+
+$(GITHOOK_DIR)/%: $(GITHOOK_SRC_DIR)/%
+	@echo installing $$(basename $@)...
+	@mkdir -p $(GITHOOK_DIR)
+	@cp $< $@
+	@chmod +x $@
 
-packages: $(patsubst %,pkg-%,$(PACKAGES))
-obitools: $(patsubst %,$(OBITOOLS_PREFIX)%,$(OBITOOLS)) 
 
 update-deps:
 	go get -u ./...
 
-test:
+test: .FORCE
 	$(GOTEST) ./...
 
-obitests: 
+obitests:
 	@for t in $$(find obitests -name test.sh -print) ; do \
-		bash $${t} ;\
+		bash $${t} || exit 1;\
-	done 
+	done
 
 githubtests: obitools obitests
-	
-man: 
-	make -C doc man
-obibook: 
-	make -C doc obibook
-doc: man obibook
-
-macos-pkg: 
-	@bash pkgs/macos/macos-installer-builder-master/macOS-x64/build-macos-x64.sh \
-		OBITools \
-		0.0.1
 
 $(BUILD_DIR):
 	mkdir -p $@
@@ -90,19 +94,61 @@ $(foreach P,$(PACKAGE_DIRS),$(eval $(call MAKE_PKG_RULE,$(P))))
 
 $(foreach P,$(OBITOOLS_DIRS),$(eval $(call MAKE_OBITOOLS_RULE,$(P))))
 
-pkg/obioptions/version.go: .FORCE
+pkg/obioptions/version.go: version.txt .FORCE
-ifneq ($(strip $(COMMIT_ID)),)
+	@version=$$(cat version.txt); \
-	@cat $@ \
+	cat $@ \
-	| sed  -E 's/^var _Commit = "[^"]*"/var _Commit = "'$(COMMIT_ID)'"/' \
+	| sed  -E 's/^var _Version = "[^"]*"/var _Version = "Release '$$version'"/' \
-	| sed  -E 's/^var _Version = "[^"]*"/var _Version = "'"$(LAST_TAG)"'"/' \
 	> $(OUTPUT)
 
 	@diff $@ $(OUTPUT) 2>&1 > /dev/null \
-    	|| echo "Update version.go : $@ to $(LAST_TAG) ($(COMMIT_ID))" \
+    	|| (echo "Update version.go to $$(cat version.txt)" && mv $(OUTPUT) $@)
-    	&& mv $(OUTPUT) $@
 
 	@rm -f $(OUTPUT)
-endif
 
-.PHONY: all packages obitools man obibook doc update-deps obitests githubtests .FORCE
+bump-version:
-.FORCE:
+	@echo "Incrementing version..."
+	@current=$$(cat version.txt); \
+	echo "  Current version: $$current"; \
+	major=$$(echo $$current | cut -d. -f1); \
+	minor=$$(echo $$current | cut -d. -f2); \
+	patch=$$(echo $$current | cut -d. -f3); \
+	new_patch=$$((patch + 1)); \
+	new_version="$$major.$$minor.$$new_patch"; \
+	echo "  New version: $$new_version"; \
+	echo "$$new_version" > version.txt
+	@echo "✓ Version updated in version.txt"
+	@$(MAKE) pkg/obioptions/version.go
+
+jjnew:
+	@echo "$(YELLOW)→ Creating a new commit...$(NC)"
+	@echo "$(BLUE)→ Documenting current commit...$(NC)"
+	@jj auto-describe
+	@echo "$(BLUE)→ Done.$(NC)"
+	@jj new
+	@echo "$(GREEN)✓ New commit created$(NC)"
+
+jjpush:
+	@echo "$(YELLOW)→ Pushing commit to repository...$(NC)"
+	@echo "$(BLUE)→ Documenting current commit...$(NC)"
+	@jj auto-describe
+	@echo "$(BLUE)→ Creating new commit for version bump...$(NC)"
+	@jj new
+	@$(MAKE) bump-version
+	@echo "$(BLUE)→ Documenting version bump commit...$(NC)"
+	@jj auto-describe
+	@version=$$(cat version.txt); \
+	tag_name="Release_$$version"; \
+	echo "$(BLUE)→ Pushing commits and creating tag $$tag_name...$(NC)"; \
+	jj git push --change @; \
+	git tag -a "$$tag_name" -m "Release $$version" 2>/dev/null || echo "Tag $$tag_name already exists"; \
+	git push origin "$$tag_name" 2>/dev/null || echo "Tag already pushed"
+	@echo "$(GREEN)✓ Commits and tag pushed to repository$(NC)"
+
+jjfetch:
+	@echo "$(YELLOW)→ Pulling latest commits...$(NC)"
+	@jj git fetch
+	@jj new master@origin
+	@echo "$(GREEN)✓ Latest commits pulled$(NC)"
+
+.PHONY: all obitools update-deps obitests githubtests jjnew jjpush jjfetch bump-version .FORCE
+.FORCE:

Release-notes.md

@@ -1,13 +1,64 @@
 # OBITools release notes
 
+## New changes
+
+### Bug fixes
+
+- In `obipairing` correct the misspelling of the `obiparing_*` tags where the `i`
+  was missing to `obipairing_`.  
+
+- In `obigrep` the **-C** option that excludes sequences too abundant was not 
+  functional.
+
+- In `obitaxonomy` the **-l** option that lists all the taxonomic rank defined by
+  a taxonomy was not functional
+
+- The file type guesser was not using enough data to be able to correctly detect
+  file format when sequences were too long in fastq and fasta or when lines were
+  to long in CSV files. That's now corrected
+
+- Options **--fasta** or **--fastq** usable to specify input format were ignored.
+  They are now correctly considered
+
+- The `obiannotate` command were crashing when a selection option was used but
+  no editing option.
+  
+- The `--fail-on-taxonomy` led to an error on merged taxa even when the
+  `--update-taxid` option was used.
+
+- The `--compressed` option was not correctly named. It was renamed to `--compress`
+  
+### Enhancement
+
+- Some sequences in the Genbank and EMBL databases are several gigabases long. The 
+  sequence parser had to reallocate and recopy memory many times to read them, 
+  resulting in a complexity of O(N^2) for reading such large sequences. 
+  The new file chunk reader has a linear algorithm that speeds up the reading 
+  of very long sequences.
+
+- A new option **--csv** is added to every obitools to indicate that the input
+  format is CSV
+
+- The new version of obitools are now printing the taxids in a fancy way
+  including the scientific name and the taxonomic rank (`"taxon:9606 [Homo
+  sapiens]@species"`). But if you need the old fashion raw taxid, a new option
+  **--raw-taxid** has been added to get obitools printing the taxids without any
+  decorations (`"9606"`).
+
+
 ## March 1st, 2025. Release 4.4.0
 
 A new documentation website is available at https://obitools4.metabarcoding.org.
 Its development is still in progress. 
 
-The biggest step forward in this new version is taxonomy management.
+The biggest step forward in this new version is taxonomy management. The new
-The new version is now able to handle taxonomic identifiers that are not just integer values. This is a first step towards an easy way to handle other taxonomy databases soon, such as the GBIF or Catalogue of Life taxonomies.
+version is now able to handle taxonomic identifiers that are not just integer
-This version is able to handle files containing taxonomic information created by previous versions of OBITools, but files created by this new version may have some problems to be analysed by previous versions, at least for the taxonomic information.
+values. This is a first step towards an easy way to handle other taxonomy
+databases soon, such as the GBIF or Catalog of Life taxonomies. This version
+is able to handle files containing taxonomic information created by previous
+versions of OBITools, but files created by this new version may have some
+problems to be analyzed by previous versions, at least for the taxonomic
+information.
 
 
 ### Breaking changes

blackboard/Prospective/kmer_index_design.md

@@ -0,0 +1,213 @@
+# Index de k-mers pour génomes de grande taille
+
+## Contexte et objectifs
+
+### Cas d'usage
+
+- Indexation de k-mers longs (k=31) pour des génomes de grande taille (< 10 Go par génome)
+- Nombre de génomes : plusieurs dizaines à quelques centaines
+- Indexation en parallèle
+- Stockage sur disque
+- Possibilité d'ajouter des génomes, mais pas de modifier un génome existant
+
+### Requêtes cibles
+
+- **Présence/absence** d'un k-mer dans un génome
+- **Intersection** entre génomes
+- **Distances** : Jaccard (présence/absence) et potentiellement Bray-Curtis (comptage)
+
+### Ressources disponibles
+
+- 128 Go de RAM
+- Stockage disque
+
+---
+
+## Estimation des volumes
+
+### Par génome
+
+- **10 Go de séquence** → ~10¹⁰ k-mers bruts (chevauchants)
+- **Après déduplication** : typiquement 10-50% de k-mers uniques → **~1-5 × 10⁹ k-mers distincts**
+
+### Espace théorique
+
+- **k=31** → 62 bits → ~4.6 × 10¹⁸ k-mers possibles
+- Table d'indexation directe impossible
+
+---
+
+## Métriques de distance
+
+### Présence/absence (binaire)
+
+- **Jaccard** : |A ∩ B| / |A ∪ B|
+- **Sørensen-Dice** : 2|A ∩ B| / (|A| + |B|)
+
+### Comptage (abondance)
+
+- **Bray-Curtis** : 1 - (2 × Σ min(aᵢ, bᵢ)) / (Σ aᵢ + Σ bᵢ)
+
+Note : Pour Bray-Curtis, le stockage des comptages est nécessaire, ce qui augmente significativement la taille de l'index.
+
+---
+
+## Options d'indexation
+
+### Option 1 : Bloom Filter par génome
+
+**Principe** : Structure probabiliste pour test d'appartenance.
+
+**Avantages :**
+- Très compact : ~10 bits/élément pour FPR ~1%
+- Construction rapide, streaming
+- Facile à sérialiser/désérialiser
+- Intersection et Jaccard estimables via formules analytiques
+
+**Inconvénients :**
+- Faux positifs (pas de faux négatifs)
+- Distances approximatives
+
+**Taille estimée** : 1-6 Go par génome (selon FPR cible)
+
+#### Dimensionnement des Bloom filters
+
+```
+\mathrm{FPR} ;=; \left(1 - e^{-h n / m}\right)^h
+```
+
+
+| Bits/élément | FPR optimal | k (hash functions) |
+|--------------|-------------|---------------------|
+| 8            | ~2%         | 5-6                 |
+| 10           | ~1%         | 7                   |
+| 12           | ~0.3%       | 8                   |
+| 16           | ~0.01%      | 11                  |
+
+Formule du taux de faux positifs :
+```
+FPR ≈ (1 - e^(-kn/m))^k
+```
+Où n = nombre d'éléments, m = nombre de bits, k = nombre de hash functions.
+
+### Option 2 : Ensemble trié de k-mers
+
+**Principe** : Stocker les k-mers (uint64) triés, avec compression possible.
+
+**Avantages :**
+- Exact (pas de faux positifs)
+- Intersection/union par merge sort O(n+m)
+- Compression efficace (delta encoding sur k-mers triés)
+
+**Inconvénients :**
+- Plus volumineux : 8 octets/k-mer
+- Construction plus lente (tri nécessaire)
+
+**Taille estimée** : 8-40 Go par génome (non compressé)
+
+### Option 3 : MPHF (Minimal Perfect Hash Function)
+
+**Principe** : Fonction de hash parfaite minimale pour les k-mers présents.
+
+**Avantages :**
+- Très compact : ~3-4 bits/élément
+- Lookup O(1)
+- Exact pour les k-mers présents
+
+**Inconvénients :**
+- Construction coûteuse (plusieurs passes)
+- Statique (pas d'ajout de k-mers après construction)
+- Ne distingue pas "absent" vs "jamais vu" sans structure auxiliaire
+
+### Option 4 : Hybride MPHF + Bloom filter
+
+- MPHF pour mapping compact des k-mers présents
+- Bloom filter pour pré-filtrage des absents
+
+---
+
+## Optimisation : Indexation de (k-2)-mers pour requêtes k-mers
+
+### Principe
+
+Au lieu d'indexer directement les 31-mers dans un Bloom filter, on indexe les 29-mers. Pour tester la présence d'un 31-mer, on vérifie que les **trois 29-mers** qu'il contient sont présents :
+
+- positions 0-28
+- positions 1-29
+- positions 2-30
+
+### Analyse probabiliste
+
+Si le Bloom filter a un FPR de p pour un 29-mer individuel, le FPR effectif pour un 31-mer devient **p³** (les trois requêtes doivent toutes être des faux positifs).
+
+| FPR 29-mer | FPR 31-mer effectif |
+|------------|---------------------|
+| 10%        | 0.1%                |
+| 5%         | 0.0125%             |
+| 1%         | 0.0001%             |
+
+### Avantages
+
+1. **Moins d'éléments à stocker** : il y a moins de 29-mers distincts que de 31-mers distincts dans un génome (deux 31-mers différents peuvent partager un même 29-mer)
+
+2. **FPR drastiquement réduit** : FPR³ avec seulement 3 requêtes
+
+3. **Index plus compact** : on peut utiliser moins de bits par élément (FPR plus élevé acceptable sur le 29-mer) tout en obtenant un FPR très bas sur le 31-mer
+
+### Trade-off
+
+Un Bloom filter à **5-6 bits/élément** pour les 29-mers donnerait un FPR effectif < 0.01% pour les 31-mers, soit environ **2× plus compact** que l'approche directe à qualité égale.
+
+**Coût** : 3× plus de requêtes par lookup (mais les requêtes Bloom sont très rapides).
+
+---
+
+## Accélération des calculs de distance : MinHash
+
+### Principe
+
+Pré-calculer une "signature" compacte (sketch) de chaque génome permettant d'estimer rapidement Jaccard sans charger les index complets.
+
+### Avantages
+
+- Matrice de distances entre 100+ génomes en quelques secondes
+- Signature de taille fixe (ex: 1000-10000 hash values) quel que soit le génome
+- Stockage minimal
+
+### Utilisation
+
+1. Construction : une passe sur les k-mers de chaque génome
+2. Distance : comparaison des sketches en O(taille du sketch)
+
+---
+
+## Architecture recommandée
+
+### Pour présence/absence + Jaccard
+
+1. **Index principal** : Bloom filter de (k-2)-mers avec l'optimisation décrite
+   - Compact (~3-5 Go par génome)
+   - FPR très bas pour les k-mers grâce aux requêtes triples
+
+2. **Sketches MinHash** : pour calcul rapide des distances entre génomes
+   - Quelques Ko par génome
+   - Permet exploration rapide de la matrice de distances
+
+### Pour comptage + Bray-Curtis
+
+1. **Index principal** : k-mers triés + comptages
+   - uint64 (k-mer) + uint8/uint16 (count)
+   - Compression delta possible
+   - Plus volumineux mais exact
+
+2. **Sketches** : variantes de MinHash pour données pondérées (ex: HyperMinHash)
+
+---
+
+## Prochaines étapes
+
+1. Implémenter un Bloom filter optimisé pour k-mers
+2. Implémenter l'optimisation (k-2)-mer → k-mer
+3. Implémenter MinHash pour les sketches
+4. Définir le format de sérialisation sur disque
+5. Benchmarker sur des génomes réels

cmd/obitools/obiannotate/main.go

@@ -30,7 +30,11 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obiannotate.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obiannotate",
+		"edits the sequence annotations",
+		obiannotate.OptionSet,
+	)
 
 	_, args := optionParser(os.Args)
 
@@ -38,6 +42,11 @@ func main() {
 	obiconvert.OpenSequenceDataErrorMessage(args, err)
 
 	annotator := obiannotate.CLIAnnotationPipeline()
+
+	if obiannotate.CLIHasSetNumberFlag() {
+		sequences = sequences.NumberSequences(1, !obiconvert.CLINoInputOrder())
+	}
+
 	obiconvert.CLIWriteBioSequences(sequences.Pipe(annotator), true)
 
 	obiutils.WaitForLastPipe()

cmd/obitools/obiclean/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obiclean.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obiclean",
+		"",
+		obiclean.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obicleandb/main.go

@@ -14,7 +14,10 @@ import (
 func main() {
 	obidefault.SetBatchSize(10)
 
-	optionParser := obioptions.GenerateOptionParser(obicleandb.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obicleandb",
+		"clean-up reference databases",
+		obicleandb.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obicomplement/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obiconvert.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obicomplement",
+		"reverse complement of sequences",
+		obiconvert.OptionSet(true))
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obiconsensus/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obiconsensus.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obiconsensus",
+		"ONT reads denoising",
+		obiconsensus.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obiconvert/main.go

@@ -14,7 +14,10 @@ func main() {
 	obidefault.SetStrictReadWorker(2)
 	obidefault.SetStrictWriteWorker(2)
 
-	optionParser := obioptions.GenerateOptionParser(obiconvert.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obiconvert",
+		"convertion of sequence files to various formats",
+		obiconvert.OptionSet(true))
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obicount/main.go

@@ -28,6 +28,8 @@ func main() {
 	// defer trace.Stop()
 
 	optionParser := obioptions.GenerateOptionParser(
+		"obicount",
+		"counts the sequences present in a file of sequences",
 		obiconvert.InputOptionSet,
 		obicount.OptionSet,
 	)

cmd/obitools/obicsv/main.go

@@ -10,7 +10,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obicsv.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obicsv",
+		"converts sequence files to CSV format",
+		obicsv.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obidemerge/main.go

@@ -15,7 +15,10 @@ func main() {
 	obidefault.SetStrictReadWorker(2)
 	obidefault.SetStrictWriteWorker(2)
 
-	optionParser := obioptions.GenerateOptionParser(obidemerge.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obidemerge",
+		"",
+		obidemerge.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obidistribute/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obidistribute.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obidistribute",
+		"divided an input set of sequences into subsets",
+		obidistribute.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obigrep/main.go

@@ -30,7 +30,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obigrep.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obigrep",
+		"select a subset of sequences on various criteria",
+		obigrep.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obijoin/main.go

@@ -15,7 +15,10 @@ func main() {
 	obidefault.SetStrictReadWorker(2)
 	obidefault.SetStrictWriteWorker(2)
 
-	optionParser := obioptions.GenerateOptionParser(obijoin.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obijoin",
+		"merge annotations contained in a file to another file",
+		obijoin.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obikmermatch/main.go

@@ -31,7 +31,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obikmersim.MatchOptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obikmermatch",
+		"",
+		obikmersim.MatchOptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obikmersimcount/main.go

@@ -32,7 +32,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obikmersim.CountOptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obikmersimcount",
+		"",
+		obikmersim.CountOptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obilandmark/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obilandmark.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obilandmark",
+		"",
+		obilandmark.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obilowmask/main.go

@@ -0,0 +1,47 @@
+package main
+
+import (
+	"os"
+
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obioptions"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obiconvert"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obilowmask"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiutils"
+)
+
+func main() {
+
+	defer obiseq.LogBioSeqStatus()
+
+	// go tool pprof -http=":8000" ./obipairing ./cpu.pprof
+	// f, err := os.Create("cpu.pprof")
+	// if err != nil {
+	// 	log.Fatal(err)
+	// }
+	// pprof.StartCPUProfile(f)
+	// defer pprof.StopCPUProfile()
+
+	// go tool trace cpu.trace
+	// ftrace, err := os.Create("cpu.trace")
+	// if err != nil {
+	// 	log.Fatal(err)
+	// }
+	// trace.Start(ftrace)
+	// defer trace.Stop()
+
+	optionParser := obioptions.GenerateOptionParser(
+		"obimicrosat",
+		"looks for microsatellites sequences in a sequence file",
+		obilowmask.OptionSet)
+
+	_, args := optionParser(os.Args)
+
+	sequences, err := obiconvert.CLIReadBioSequences(args...)
+	obiconvert.OpenSequenceDataErrorMessage(args, err)
+
+	selected := obilowmask.CLISequenceEntropyMasker(sequences)
+	obiconvert.CLIWriteBioSequences(selected, true)
+	obiutils.WaitForLastPipe()
+
+}

cmd/obitools/obimatrix/main.go

@@ -31,6 +31,8 @@ func main() {
 	// defer trace.Stop()
 
 	optionParser := obioptions.GenerateOptionParser(
+		"obimatrix",
+		"",
 		obimatrix.OptionSet,
 	)
 

cmd/obitools/obimicrosat/main.go

@@ -30,7 +30,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obimicrosat.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obimicrosat",
+		"looks for microsatellites sequences in a sequence file",
+		obimicrosat.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obimultiplex/main.go

@@ -28,7 +28,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obimultiplex.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obimultiplex",
+		"demultiplex amplicons",
+		obimultiplex.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obipairing/main.go

@@ -30,7 +30,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obipairing.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obipairing",
+		"align forward with reverse reads with paired reads",
+		obipairing.OptionSet)
 
 	optionParser(os.Args)
 

cmd/obitools/obipcr/main.go

@@ -29,7 +29,10 @@ func main() {
 	obidefault.SetParallelFilesRead(obidefault.ParallelWorkers() / 4)
 	obidefault.SetBatchSize(10)
 
-	optionParser := obioptions.GenerateOptionParser(obipcr.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obipcr",
+		"simulates a PCR on a sequence files",
+		obipcr.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obireffamidx/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obirefidx.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obireffamidx",
+		"",
+		obirefidx.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obirefidx/main.go

@@ -11,7 +11,10 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obirefidx.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obirefidx",
+		"",
+		obirefidx.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obiscript/main.go

@@ -31,7 +31,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obiscript.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obiscript",
+		"executes a lua script on the input sequences",
+		obiscript.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obisplit/main.go

@@ -31,7 +31,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obisplit.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obisplit",
+		"",
+		obisplit.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obisummary/main.go

@@ -33,7 +33,10 @@ func main() {
 	// trace.Start(ftrace)
 	// defer trace.Stop()
 
-	optionParser := obioptions.GenerateOptionParser(obisummary.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obisummary",
+		"resume main information from a sequence file",
+		obisummary.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/obitools/obitag/main.go

@@ -11,6 +11,7 @@ import (
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitax"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obiconvert"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obitag"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obitaxonomy"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiutils"
 
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obioptions"
@@ -39,7 +40,10 @@ func main() {
 	obidefault.SetStrictWriteWorker(1)
 	obidefault.SetBatchSize(10)
 
-	optionParser := obioptions.GenerateOptionParser(obitag.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obitag",
+		"realizes taxonomic assignment",
+		obitag.OptionSet)
 
 	_, args := optionParser(os.Args)
 
@@ -55,7 +59,7 @@ func main() {
 	}
 
 	if taxo == nil {
-		taxo, err = references.ExtractTaxonomy(nil)
+		taxo, err = references.ExtractTaxonomy(nil, obitaxonomy.CLINewickWithLeaves())
 
 		if err != nil {
 			log.Fatalf("No taxonomy specified or extractable from reference database: %v", err)
@@ -70,10 +74,12 @@ func main() {
 
 	var identified obiiter.IBioSequence
 
+	fsrb := fs.Rebatch(obidefault.BatchSize())
+
 	if obitag.CLIGeometricMode() {
-		identified = obitag.CLIGeomAssignTaxonomy(fs, references, taxo)
+		identified = obitag.CLIGeomAssignTaxonomy(fsrb, references, taxo)
 	} else {
-		identified = obitag.CLIAssignTaxonomy(fs, references, taxo)
+		identified = obitag.CLIAssignTaxonomy(fsrb, references, taxo)
 	}
 
 	obiconvert.CLIWriteBioSequences(identified, true)

cmd/obitools/obitagpcr/main.go

@@ -33,7 +33,10 @@ func main() {
 
 	obidefault.SetWorkerPerCore(1)
 
-	optionParser := obioptions.GenerateOptionParser(obitagpcr.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obitagpcr",
+		"split a paired raw read data set per sample",
+		obitagpcr.OptionSet)
 
 	optionParser(os.Args)
 	pairs, err := obipairing.CLIPairedSequence()

cmd/obitools/obitaxonomy/main.go

@@ -4,9 +4,11 @@ import (
 	"os"
 
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obidefault"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiitercsv"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obioptions"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitax"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obiconvert"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obicsv"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitools/obitaxonomy"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiutils"
 
@@ -14,30 +16,59 @@ import (
 )
 
 func main() {
-	optionParser := obioptions.GenerateOptionParser(obitaxonomy.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obitaxonomy",
+		"manipulates and queries taxonomy",
+		obitaxonomy.OptionSet)
 
 	_, args := optionParser(os.Args)
 
 	var iterator *obitax.ITaxon
 
-	switch {
+	if obitaxonomy.CLIDownloadNCBI() {
-	case obitaxonomy.CLIDownloadNCBI():
 		err := obitaxonomy.CLIDownloadNCBITaxdump()
 		if err != nil {
 			log.Errorf("Cannot download NCBI taxonomy: %s", err.Error())
 			os.Exit(1)
 		}
+		os.Exit(0)
+	}
 
+	if !obidefault.HasSelectedTaxonomy() {
+		log.Fatal("you must indicate a taxonomy using the -t or --taxonomy option")
+	}
+
+	switch {
+	case obitaxonomy.CLIAskForRankList():
+		newIter := obiitercsv.NewICSVRecord()
+		newIter.Add(1)
+		newIter.AppendField("rank")
+		go func() {
+			ranks := obitax.DefaultTaxonomy().RankList()
+			data := make([]obiitercsv.CSVRecord, len(ranks))
+
+			for i, rank := range ranks {
+				record := make(obiitercsv.CSVRecord)
+				record["rank"] = rank
+				data[i] = record
+			}
+			newIter.Push(obiitercsv.MakeCSVRecordBatch(obitax.DefaultTaxonomy().Name(), 0, data))
+			newIter.Close()
+			newIter.Done()
+		}()
+		obicsv.CLICSVWriter(newIter, true)
+		obiutils.WaitForLastPipe()
 		os.Exit(0)
 
 	case obitaxonomy.CLIExtractTaxonomy():
 		iter, err := obiconvert.CLIReadBioSequences(args...)
+		iter = iter.NumberSequences(1, true)
 
 		if err != nil {
 			log.Fatalf("Cannot extract taxonomy: %v", err)
 		}
 
-		taxonomy, err := iter.ExtractTaxonomy()
+		taxonomy, err := iter.ExtractTaxonomy(obitaxonomy.CLINewickWithLeaves())
 
 		if err != nil {
 			log.Fatalf("Cannot extract taxonomy: %v", err)
@@ -99,7 +130,12 @@ func main() {
 	}
 
 	iterator = obitaxonomy.CLITaxonRestrictions(iterator)
-	obitaxonomy.CLICSVTaxaWriter(iterator, true)
+
+	if obitaxonomy.CLIAsNewick() {
+		obitaxonomy.CLINewickWriter(iterator, true)
+	} else {
+		obitaxonomy.CLICSVTaxaWriter(iterator, true)
+	}
 
 	obiutils.WaitForLastPipe()
 

cmd/obitools/obiuniq/main.go

@@ -33,7 +33,10 @@ func main() {
 
 	obidefault.SetBatchSize(10)
 	obidefault.SetReadQualities(false)
-	optionParser := obioptions.GenerateOptionParser(obiuniq.OptionSet)
+	optionParser := obioptions.GenerateOptionParser(
+		"obiuniq",
+		"dereplicate sequence data sets",
+		obiuniq.OptionSet)
 
 	_, args := optionParser(os.Args)
 

cmd/test/main.go

@@ -3,13 +3,13 @@ package main
 import (
 	"os"
 
-	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obitax"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiformats"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiutils"
 )
 
 func main() {
 
-	obitax.DetectTaxonomyFormat(os.Args[1])
+	obiformats.DetectTaxonomyFormat(os.Args[1])
 	println(obiutils.RemoveAllExt("toto/tutu/test.txt"))
 	println(obiutils.Basename("toto/tutu/test.txt"))
 

git-hooks/pre-push

@@ -0,0 +1,23 @@
+#!/bin/bash
+
+remote="$1"
+#url="$2"
+
+log() {
+    echo -e "[Pre-Push tests @ $(date)] $*" 1>&2
+}
+
+current_branch=$(git symbolic-ref --short head)
+
+cmd="make githubtests"
+
+if [[ $current_branch = "master" ]]; then
+    log "you are on $current_branch, running build test"
+    if ! eval "$cmd"; then
+        log "Pre-push tests failed $cmd" 
+        exit 1
+    fi
+fi
+
+log "Tests are OK, ready to push on $remote"
+exit 0

go.mod

@@ -1,11 +1,12 @@
 module git.metabarcoding.org/obitools/obitools4/obitools4
 
-go 1.23.1
+go 1.23.4
+
+toolchain go1.24.2
 
 require (
 	github.com/DavidGamba/go-getoptions v0.28.0
 	github.com/PaesslerAG/gval v1.2.2
-	github.com/TuftsBCB/io v0.0.0-20140121014543-22b94e9b23f9
 	github.com/barkimedes/go-deepcopy v0.0.0-20220514131651-17c30cfc62df
 	github.com/buger/jsonparser v1.1.1
 	github.com/chen3feng/stl4go v0.1.1
@@ -19,19 +20,23 @@ require (
 	github.com/stretchr/testify v1.8.4
 	github.com/tevino/abool/v2 v2.1.0
 	github.com/yuin/gopher-lua v1.1.1
-	golang.org/x/exp v0.0.0-20231006140011-7918f672742d
+	golang.org/x/exp v0.0.0-20231110203233-9a3e6036ecaa
 	gonum.org/v1/gonum v0.14.0
 	gopkg.in/yaml.v3 v3.0.1
 	scientificgo.org/special v0.0.0
 )
 
 require (
+	github.com/RoaringBitmap/roaring v1.9.4 // indirect
+	github.com/bits-and-blooms/bitset v1.12.0 // indirect
 	github.com/davecgh/go-spew v1.1.1 // indirect
 	github.com/goombaio/orderedmap v0.0.0-20180924084748-ba921b7e2419 // indirect
-	github.com/kr/pretty v0.3.0 // indirect
+	github.com/kr/pretty v0.3.1 // indirect
 	github.com/kr/text v0.2.0 // indirect
+	github.com/mschoch/smat v0.2.0 // indirect
+	github.com/pelletier/go-toml/v2 v2.2.4 // indirect
 	github.com/pmezard/go-difflib v1.0.0 // indirect
-	github.com/rogpeppe/go-internal v1.6.1 // indirect
+	github.com/rogpeppe/go-internal v1.12.0 // indirect
 )
 
 require (
@@ -44,8 +49,8 @@ require (
 	github.com/rivo/uniseg v0.4.4 // indirect
 	github.com/shopspring/decimal v1.3.1 // indirect
 	github.com/ulikunitz/xz v0.5.11
-	golang.org/x/net v0.17.0 // indirect
+	golang.org/x/net v0.35.0 // indirect
-	golang.org/x/sys v0.17.0 // indirect
+	golang.org/x/sys v0.30.0 // indirect
-	golang.org/x/term v0.13.0 // indirect
+	golang.org/x/term v0.29.0 // indirect
 	gopkg.in/check.v1 v1.0.0-20201130134442-10cb98267c6c
 )

go.sum

@@ -4,10 +4,12 @@ github.com/PaesslerAG/gval v1.2.2 h1:Y7iBzhgE09IGTt5QgGQ2IdaYYYOU134YGHBThD+wm9E
 github.com/PaesslerAG/gval v1.2.2/go.mod h1:XRFLwvmkTEdYziLdaCeCa5ImcGVrfQbeNUbVR+C6xac=
 github.com/PaesslerAG/jsonpath v0.1.0 h1:gADYeifvlqK3R3i2cR5B4DGgxLXIPb3TRTH1mGi0jPI=
 github.com/PaesslerAG/jsonpath v0.1.0/go.mod h1:4BzmtoM/PI8fPO4aQGIusjGxGir2BzcV0grWtFzq1Y8=
-github.com/TuftsBCB/io v0.0.0-20140121014543-22b94e9b23f9 h1:Zc1/GNsUpgZR9qm1EmRSKrnOHA7CCd0bIzGdq0cREN0=
+github.com/RoaringBitmap/roaring v1.9.4 h1:yhEIoH4YezLYT04s1nHehNO64EKFTop/wBhxv2QzDdQ=
-github.com/TuftsBCB/io v0.0.0-20140121014543-22b94e9b23f9/go.mod h1:PZyV4WA3NpqtezSY0h6E6NARAmdDm0qwrydveOyR5Gc=
+github.com/RoaringBitmap/roaring v1.9.4/go.mod h1:6AXUsoIEzDTFFQCe1RbGA6uFONMhvejWj5rqITANK90=
 github.com/barkimedes/go-deepcopy v0.0.0-20220514131651-17c30cfc62df h1:GSoSVRLoBaFpOOds6QyY1L8AX7uoY+Ln3BHc22W40X0=
 github.com/barkimedes/go-deepcopy v0.0.0-20220514131651-17c30cfc62df/go.mod h1:hiVxq5OP2bUGBRNS3Z/bt/reCLFNbdcST6gISi1fiOM=
+github.com/bits-and-blooms/bitset v1.12.0 h1:U/q1fAF7xXRhFCrhROzIfffYnu+dlS38vCZtmFVPHmA=
+github.com/bits-and-blooms/bitset v1.12.0/go.mod h1:7hO7Gc7Pp1vODcmWvKMRA9BNmbv6a/7QIWpPxHddWR8=
 github.com/buger/jsonparser v1.1.1 h1:2PnMjfWD7wBILjqQbt530v576A/cAbQvEW9gGIpYMUs=
 github.com/buger/jsonparser v1.1.1/go.mod h1:6RYKKt7H4d4+iWqouImQ9R2FZql3VbhNgx27UK13J/0=
 github.com/chen3feng/stl4go v0.1.1 h1:0L1+mDw7pomftKDruM23f1mA7miavOj6C6MZeadzN2Q=
@@ -36,10 +38,9 @@ github.com/klauspost/compress v1.17.2/go.mod h1:ntbaceVETuRiXiv4DpjP66DpAtAGkEQs
 github.com/klauspost/cpuid v1.2.0/go.mod h1:Pj4uuM528wm8OyEC2QMXAi2YiTZ96dNQPGgoMS4s3ek=
 github.com/klauspost/pgzip v1.2.6 h1:8RXeL5crjEUFnR2/Sn6GJNWtSQ3Dk8pq4CL3jvdDyjU=
 github.com/klauspost/pgzip v1.2.6/go.mod h1:Ch1tH69qFZu15pkjo5kYi6mth2Zzwzt50oCQKQE9RUs=
-github.com/kr/pretty v0.1.0/go.mod h1:dAy3ld7l9f0ibDNOQOHHMYYIIbhfbHSm3C4ZsoJORNo=
 github.com/kr/pretty v0.2.1/go.mod h1:ipq/a2n7PKx3OHsz4KJII5eveXtPO4qwEXGdVfWzfnI=
-github.com/kr/pretty v0.3.0 h1:WgNl7dwNpEZ6jJ9k1snq4pZsg7DOEN8hP9Xw0Tsjwk0=
+github.com/kr/pretty v0.3.1 h1:flRD4NNwYAUpkphVc1HcthR4KEIFJ65n8Mw5qdRn3LE=
-github.com/kr/pretty v0.3.0/go.mod h1:640gp4NfQd8pI5XOwp5fnNeVWj67G7CFk/SaSQn7NBk=
+github.com/kr/pretty v0.3.1/go.mod h1:hoEshYVHaxMs3cyo3Yncou5ZscifuDolrwPKZanG3xk=
 github.com/kr/pty v1.1.1/go.mod h1:pFQYn66WHrOpPYNljwOMqo10TkYh1fy3cYio2l3bCsQ=
 github.com/kr/text v0.1.0/go.mod h1:4Jbv+DJW3UT/LiOwJeYQe1efqtUx/iVham/4vfdArNI=
 github.com/kr/text v0.2.0 h1:5Nx0Ya0ZqY2ygV366QzturHI13Jq95ApcVaJBhpS+AY=
@@ -50,15 +51,21 @@ github.com/mattn/go-runewidth v0.0.15 h1:UNAjwbU9l54TA3KzvqLGxwWjHmMgBUVhBiTjelZ
 github.com/mattn/go-runewidth v0.0.15/go.mod h1:Jdepj2loyihRzMpdS35Xk/zdY8IAYHsh153qUoGf23w=
 github.com/mitchellh/colorstring v0.0.0-20190213212951-d06e56a500db h1:62I3jR2EmQ4l5rM/4FEfDWcRD+abF5XlKShorW5LRoQ=
 github.com/mitchellh/colorstring v0.0.0-20190213212951-d06e56a500db/go.mod h1:l0dey0ia/Uv7NcFFVbCLtqEBQbrT4OCwCSKTEv6enCw=
+github.com/mschoch/smat v0.2.0 h1:8imxQsjDm8yFEAVBe7azKmKSgzSkZXDuKkSq9374khM=
+github.com/mschoch/smat v0.2.0/go.mod h1:kc9mz7DoBKqDyiRL7VZN8KvXQMWeTaVnttLRXOlotKw=
 github.com/pbnjay/memory v0.0.0-20210728143218-7b4eea64cf58 h1:onHthvaw9LFnH4t2DcNVpwGmV9E1BkGknEliJkfwQj0=
 github.com/pbnjay/memory v0.0.0-20210728143218-7b4eea64cf58/go.mod h1:DXv8WO4yhMYhSNPKjeNKa5WY9YCIEBRbNzFFPJbWO6Y=
+github.com/pelletier/go-toml/v2 v2.2.4 h1:mye9XuhQ6gvn5h28+VilKrrPoQVanw5PMw/TB0t5Ec4=
+github.com/pelletier/go-toml/v2 v2.2.4/go.mod h1:2gIqNv+qfxSVS7cM2xJQKtLSTLUE9V8t9Stt+h56mCY=
+github.com/pkg/diff v0.0.0-20210226163009-20ebb0f2a09e/go.mod h1:pJLUxLENpZxwdsKMEsNbx1VGcRFpLqf3715MtcvvzbA=
 github.com/pmezard/go-difflib v1.0.0 h1:4DBwDE0NGyQoBHbLQYPwSUPoCMWR5BEzIk/f1lZbAQM=
 github.com/pmezard/go-difflib v1.0.0/go.mod h1:iKH77koFhYxTK1pcRnkKkqfTogsbg7gZNVY4sRDYZ/4=
 github.com/rivo/uniseg v0.2.0/go.mod h1:J6wj4VEh+S6ZtnVlnTBMWIodfgj8LQOQFoIToxlJtxc=
 github.com/rivo/uniseg v0.4.4 h1:8TfxU8dW6PdqD27gjM8MVNuicgxIjxpm4K7x4jp8sis=
 github.com/rivo/uniseg v0.4.4/go.mod h1:FN3SvrM+Zdj16jyLfmOkMNblXMcoc8DfTHruCPUcx88=
-github.com/rogpeppe/go-internal v1.6.1 h1:/FiVV8dS/e+YqF2JvO3yXRFbBLTIuSDkuC7aBOAvL+k=
+github.com/rogpeppe/go-internal v1.9.0/go.mod h1:WtVeX8xhTBvf0smdhujwtBcq4Qrzq/fJaraNFVN+nFs=
-github.com/rogpeppe/go-internal v1.6.1/go.mod h1:xXDCJY+GAPziupqXw64V24skbSoqbTEfhy4qGm1nDQc=
+github.com/rogpeppe/go-internal v1.12.0 h1:exVL4IDcn6na9z1rAb56Vxr+CgyK3nn3O+epU5NdKM8=
+github.com/rogpeppe/go-internal v1.12.0/go.mod h1:E+RYuTGaKKdloAfM02xzb0FW3Paa99yedzYV+kq4uf4=
 github.com/rrethy/ahocorasick v1.0.0 h1:YKkCB+E5PXc0xmLfMrWbfNht8vG9Re97IHSWZk/Lk8E=
 github.com/rrethy/ahocorasick v1.0.0/go.mod h1:nq8oScE7Vy1rOppoQxpQiiDmPHuKCuk9rXrNcxUV3R0=
 github.com/schollz/progressbar/v3 v3.13.1 h1:o8rySDYiQ59Mwzy2FELeHY5ZARXZTVJC7iHD6PEFUiE=
@@ -79,25 +86,23 @@ github.com/ulikunitz/xz v0.5.11 h1:kpFauv27b6ynzBNT/Xy+1k+fK4WswhN/6PN5WhFAGw8=
 github.com/ulikunitz/xz v0.5.11/go.mod h1:nbz6k7qbPmH4IRqmfOplQw/tblSgqTqBwxkY0oWt/14=
 github.com/yuin/gopher-lua v1.1.1 h1:kYKnWBjvbNP4XLT3+bPEwAXJx262OhaHDWDVOPjL46M=
 github.com/yuin/gopher-lua v1.1.1/go.mod h1:GBR0iDaNXjAgGg9zfCvksxSRnQx76gclCIb7kdAd1Pw=
-golang.org/x/exp v0.0.0-20231006140011-7918f672742d h1:jtJma62tbqLibJ5sFQz8bKtEM8rJBtfilJ2qTU199MI=
+golang.org/x/exp v0.0.0-20231110203233-9a3e6036ecaa h1:FRnLl4eNAQl8hwxVVC17teOw8kdjVDVAiFMtgUdTSRQ=
-golang.org/x/exp v0.0.0-20231006140011-7918f672742d/go.mod h1:ldy0pHrwJyGW56pPQzzkH36rKxoZW1tw7ZJpeKx+hdo=
+golang.org/x/exp v0.0.0-20231110203233-9a3e6036ecaa/go.mod h1:zk2irFbV9DP96SEBUUAy67IdHUaZuSnrz1n472HUCLE=
-golang.org/x/net v0.17.0 h1:pVaXccu2ozPjCXewfr1S7xza/zcXTity9cCdXQYSjIM=
+golang.org/x/net v0.35.0 h1:T5GQRQb2y08kTAByq9L4/bz8cipCdA8FbRTXewonqY8=
-golang.org/x/net v0.17.0/go.mod h1:NxSsAGuq816PNPmqtQdLE42eU2Fs7NoRIZrHJAlaCOE=
+golang.org/x/net v0.35.0/go.mod h1:EglIi67kWsHKlRzzVMUD93VMSWGFOMSZgxFjparz1Qk=
 golang.org/x/sys v0.0.0-20220715151400-c0bba94af5f8/go.mod h1:oPkhp1MJrh7nUepCBck5+mAzfO9JrbApNNgaTdGDITg=
 golang.org/x/sys v0.0.0-20220811171246-fbc7d0a398ab/go.mod h1:oPkhp1MJrh7nUepCBck5+mAzfO9JrbApNNgaTdGDITg=
 golang.org/x/sys v0.6.0/go.mod h1:oPkhp1MJrh7nUepCBck5+mAzfO9JrbApNNgaTdGDITg=
-golang.org/x/sys v0.17.0 h1:25cE3gD+tdBA7lp7QfhuV+rJiE9YXTcS3VG1SqssI/Y=
+golang.org/x/sys v0.30.0 h1:QjkSwP/36a20jFYWkSue1YwXzLmsV5Gfq7Eiy72C1uc=
-golang.org/x/sys v0.17.0/go.mod h1:/VUhepiaJMQUp4+oa/7Zr1D23ma6VTLIYjOOTFZPUcA=
+golang.org/x/sys v0.30.0/go.mod h1:/VUhepiaJMQUp4+oa/7Zr1D23ma6VTLIYjOOTFZPUcA=
 golang.org/x/term v0.6.0/go.mod h1:m6U89DPEgQRMq3DNkDClhWw02AUbt2daBVO4cn4Hv9U=
-golang.org/x/term v0.13.0 h1:bb+I9cTfFazGW51MZqBVmZy7+JEJMouUHTUSKVQLBek=
+golang.org/x/term v0.29.0 h1:L6pJp37ocefwRRtYPKSWOWzOtWSxVajvz2ldH/xi3iU=
-golang.org/x/term v0.13.0/go.mod h1:LTmsnFJwVN6bCy1rVCoS+qHT1HhALEFxKncY3WNNh4U=
+golang.org/x/term v0.29.0/go.mod h1:6bl4lRlvVuDgSf3179VpIxBF0o10JUpXWOnI7nErv7s=
 gonum.org/v1/gonum v0.14.0 h1:2NiG67LD1tEH0D7kM+ps2V+fXmsAnpUeec7n8tcr4S0=
 gonum.org/v1/gonum v0.14.0/go.mod h1:AoWeoz0becf9QMWtE8iWXNXc27fK4fNeHNf/oMejGfU=
 gopkg.in/check.v1 v0.0.0-20161208181325-20d25e280405/go.mod h1:Co6ibVJAznAaIkqp8huTwlJQCZ016jof/cbN4VW5Yz0=
-gopkg.in/check.v1 v1.0.0-20180628173108-788fd7840127/go.mod h1:Co6ibVJAznAaIkqp8huTwlJQCZ016jof/cbN4VW5Yz0=
 gopkg.in/check.v1 v1.0.0-20201130134442-10cb98267c6c h1:Hei/4ADfdWqJk1ZMxUNpqntNwaWcugrBjAiHlqqRiVk=
 gopkg.in/check.v1 v1.0.0-20201130134442-10cb98267c6c/go.mod h1:JHkPIbrfpd72SG/EVd6muEfDQjcINNoR0C8j2r3qZ4Q=
-gopkg.in/errgo.v2 v2.1.0/go.mod h1:hNsd1EY+bozCKY1Ytp96fpM3vjJbqLJn88ws8XvfDNI=
 gopkg.in/yaml.v3 v3.0.0-20200313102051-9f266ea9e77c/go.mod h1:K4uyk7z7BCEPqu6E+C64Yfv1cQ7kz7rIZviUmN+EgEM=
 gopkg.in/yaml.v3 v3.0.1 h1:fxVm/GzAzEWqLHuvctI91KS9hhNmmWOoWu0XTYJS7CA=
 gopkg.in/yaml.v3 v3.0.1/go.mod h1:K4uyk7z7BCEPqu6E+C64Yfv1cQ7kz7rIZviUmN+EgEM=

go.work

@@ -1,3 +1,5 @@
-go 1.23.1
+go 1.23.4
+
+toolchain go1.24.2
 
 use .

go.work.sum

@@ -2,7 +2,6 @@ git.sr.ht/~sbinet/gg v0.3.1 h1:LNhjNn8DerC8f9DHLz6lS0YYul/b602DUxDgGkd/Aik=
 git.sr.ht/~sbinet/gg v0.3.1/go.mod h1:KGYtlADtqsqANL9ueOFkWymvzUvLMQllU5Ixo+8v3pc=
 github.com/ajstarks/svgo v0.0.0-20211024235047-1546f124cd8b h1:slYM766cy2nI3BwyRiyQj/Ud48djTMtMebDqepE95rw=
 github.com/ajstarks/svgo v0.0.0-20211024235047-1546f124cd8b/go.mod h1:1KcenG0jGWcpt8ov532z81sp/kMMUG485J2InIOyADM=
-github.com/buger/jsonparser v1.1.1/go.mod h1:6RYKKt7H4d4+iWqouImQ9R2FZql3VbhNgx27UK13J/0=
 github.com/chzyer/logex v1.1.10 h1:Swpa1K6QvQznwJRcfTfQJmTE72DqScAa40E+fbHEXEE=
 github.com/chzyer/logex v1.1.10/go.mod h1:+Ywpsq7O8HXn0nuIou7OrIPyXbp3wmkHB+jjWRnGsAI=
 github.com/chzyer/logex v1.2.0 h1:+eqR0HfOetur4tgnC8ftU5imRnhi4te+BadWS95c5AM=
@@ -20,15 +19,20 @@ github.com/go-latex/latex v0.0.0-20230307184459-12ec69307ad9 h1:NxXI5pTAtpEaU49b
 github.com/go-latex/latex v0.0.0-20230307184459-12ec69307ad9/go.mod h1:gWuR/CrFDDeVRFQwHPvsv9soJVB/iqymhuZQuJ3a9OM=
 github.com/go-pdf/fpdf v0.6.0 h1:MlgtGIfsdMEEQJr2le6b/HNr1ZlQwxyWr77r2aj2U/8=
 github.com/go-pdf/fpdf v0.6.0/go.mod h1:HzcnA+A23uwogo0tp9yU+l3V+KXhiESpt1PMayhOh5M=
+github.com/go-quicktest/qt v1.101.0/go.mod h1:14Bz/f7NwaXPtdYEgzsx46kqSxVwTbzVZsDC26tQJow=
 github.com/goccmack/gocc v0.0.0-20230228185258-2292f9e40198 h1:FSii2UQeSLngl3jFoR4tUKZLprO7qUlh/TKKticc0BM=
 github.com/goccmack/gocc v0.0.0-20230228185258-2292f9e40198/go.mod h1:DTh/Y2+NbnOVVoypCCQrovMPDKUGp4yZpSbWg5D0XIM=
 github.com/golang/freetype v0.0.0-20170609003504-e2365dfdc4a0 h1:DACJavvAHhabrF08vX0COfcOBJRhZ8lUbR+ZWIs0Y5g=
 github.com/golang/freetype v0.0.0-20170609003504-e2365dfdc4a0/go.mod h1:E/TSTwGwJL78qG/PmXZO1EjYhfJinVAhrmmHX6Z8B9k=
+github.com/google/go-cmdtest v0.4.1-0.20220921163831-55ab3332a786/go.mod h1:apVn/GCasLZUVpAJ6oWAuyP7Ne7CEsQbTnc0plM3m+o=
 github.com/google/go-cmp v0.5.8 h1:e6P7q2lk1O+qJJb4BtCQXlK8vWEO8V1ZeuEdJNOqZyg=
 github.com/google/go-cmp v0.5.8/go.mod h1:17dUlkBOakJ0+DkrSSNjCkIjxS6bF9zb3elmeNGIjoY=
+github.com/google/renameio v0.1.0/go.mod h1:KWCgfxg9yswjAJkECMjeO8J8rahYeXnNhOm40UhjYkI=
+github.com/google/safehtml v0.1.0/go.mod h1:L4KWwDsUJdECRAEpZoBn3O64bQaywRscowZjJAzjHnU=
 github.com/hashicorp/errwrap v1.0.0/go.mod h1:YH+1FKiLXxHSkmPseP+kNlulaMuP3n2brvKWEqk/Jc4=
 github.com/hashicorp/go-multierror v1.1.1/go.mod h1:iw975J/qwKPdAO1clOe2L8331t/9/fmwbPZ6JB6eMoM=
 github.com/ianlancetaylor/demangle v0.0.0-20220319035150-800ac71e25c2 h1:rcanfLhLDA8nozr/K289V1zcntHr3V+SHlXwzz1ZI2g=
+github.com/jba/templatecheck v0.7.1/go.mod h1:n1Etw+Rrw1mDDD8dDRsEKTwMZsJ98EkktgNJC6wLUGo=
 github.com/k0kubun/go-ansi v0.0.0-20180517002512-3bf9e2903213 h1:qGQQKEcAR99REcMpsXCp3lJ03zYT1PkRd3kQGPn9GVg=
 github.com/klauspost/cpuid v1.2.0 h1:NMpwD2G9JSFOE1/TJjGSo5zG7Yb2bTe7eq1jH+irmeE=
 github.com/kr/pty v1.1.1 h1:VkoXIwSboBpnk99O/KFauAEILuNHv5DVFKZMBN/gUgw=
@@ -39,17 +43,22 @@ github.com/stretchr/objx v0.1.0 h1:4G4v2dO3VZwixGIRoQ5Lfboy6nUhCyYzaqnIAPPhYs4=
 github.com/stretchr/objx v0.5.0 h1:1zr/of2m5FGMsad5YfcqgdqdWrIhu+EBEJRhR1U7z/c=
 github.com/stretchr/objx v0.5.0/go.mod h1:Yh+to48EsGEfYuaHDzXPcE3xhTkx73EhmCGUpEOglKo=
 github.com/yuin/goldmark v1.4.13 h1:fVcFKWvrslecOb/tg+Cc05dkeYx540o0FuFt3nUVDoE=
+github.com/yuin/goldmark v1.4.13/go.mod h1:6yULJ656Px+3vBD8DxQVa3kxgyrAnzto9xy5taEt/CY=
 golang.org/x/crypto v0.14.0 h1:wBqGXzWJW6m1XrIKlAH0Hs1JJ7+9KBwnIO8v66Q9cHc=
 golang.org/x/crypto v0.14.0/go.mod h1:MVFd36DqK4CsrnJYDkBA3VC4m2GkXAM0PvzMCn4JQf4=
+golang.org/x/crypto v0.33.0/go.mod h1:bVdXmD7IV/4GdElGPozy6U7lWdRXA4qyRVGJV57uQ5M=
 golang.org/x/image v0.6.0 h1:bR8b5okrPI3g/gyZakLZHeWxAR8Dn5CyxXv1hLH5g/4=
 golang.org/x/image v0.6.0/go.mod h1:MXLdDR43H7cDJq5GEGXEVeeNhPgi+YYEQ2pC1byI1x0=
 golang.org/x/mod v0.13.0 h1:I/DsJXRlw/8l/0c24sM9yb0T4z9liZTduXvdAWYiysY=
 golang.org/x/mod v0.13.0/go.mod h1:hTbmBsO62+eylJbnUtE2MGJUyE7QWk4xUqPFrRgJ+7c=
+golang.org/x/mod v0.14.0/go.mod h1:hTbmBsO62+eylJbnUtE2MGJUyE7QWk4xUqPFrRgJ+7c=
 golang.org/x/sync v0.0.0-20220722155255-886fb9371eb4 h1:uVc8UZUe6tr40fFVnUP5Oj+veunVezqYl9z7DYw9xzw=
 golang.org/x/text v0.13.0 h1:ablQoSUd0tRdKxZewP80B+BaqeKJuVhuRxj/dkrun3k=
 golang.org/x/text v0.13.0/go.mod h1:TvPlkZtksWOMsz7fbANvkp4WM8x/WCo/om8BMLbz+aE=
+golang.org/x/text v0.22.0/go.mod h1:YRoo4H8PVmsu+E3Ou7cqLVH8oXWIHVoX0jqUWALQhfY=
 golang.org/x/tools v0.14.0 h1:jvNa2pY0M4r62jkRQ6RwEZZyPcymeL9XZMLBbV7U2nc=
 golang.org/x/tools v0.14.0/go.mod h1:uYBEerGOWcJyEORxN+Ek8+TT266gXkNlHdJBwexUsBg=
+golang.org/x/tools v0.15.0/go.mod h1:hpksKq4dtpQWS1uQ61JkdqWM3LscIS6Slf+VVkm+wQk=
 golang.org/x/xerrors v0.0.0-20190717185122-a985d3407aa7 h1:9zdDQZ7Thm29KFXgAX/+yaf3eVbP7djjWp/dXAppNCc=
 gonum.org/v1/plot v0.10.1 h1:dnifSs43YJuNMDzB7v8wV64O4ABBHReuAVAoBxqBqS4=
 gonum.org/v1/plot v0.10.1/go.mod h1:VZW5OlhkL1mysU9vaqNHnsy86inf6Ot+jB3r+BczCEo=

install_obitools.sh

@@ -59,6 +59,11 @@ if [[ ! "$WORK_DIR" || ! -d "$WORK_DIR" ]]; then
   exit 1
 fi
 
+mkdir -p "${WORK_DIR}/cache" \
+  || (echo "Cannot create ${WORK_DIR}/cache directory" 1>&2  
+      exit 1)
+      
+
 mkdir -p "${INSTALL_DIR}/bin" 2> /dev/null \
   || (echo "Please enter your password for installing obitools in ${INSTALL_DIR}"  1>&2
       sudo mkdir -p "${INSTALL_DIR}/bin")
@@ -68,11 +73,11 @@ if [[ ! -d "${INSTALL_DIR}/bin" ]]; then
   exit 1
 fi
 
-INSTALL_DIR="$(cd $INSTALL_DIR && pwd)"
+INSTALL_DIR="$(cd ${INSTALL_DIR} && pwd)"
 
-echo WORK_DIR=$WORK_DIR  1>&2
+echo "WORK_DIR=$WORK_DIR"  1>&2
-echo INSTALL_DIR=$INSTALL_DIR  1>&2
+echo "INSTALL_DIR=$INSTALL_DIR"  1>&2
-echo OBITOOLS_PREFIX=$OBITOOLS_PREFIX  1>&2
+echo "OBITOOLS_PREFIX=$OBITOOLS_PREFIX"  1>&2
 
 pushd "$WORK_DIR"|| exit
 
@@ -105,6 +110,13 @@ curl "$GOURL" \
 
 PATH="$(pwd)/go/bin:$PATH"
 export PATH
+GOPATH="$(pwd)/go"
+export GOPATH
+
+export GOCACHE="$(pwd)/cache"
+echo "GOCACHE=$GOCACHE" 1>&2@
+mkdir -p "$GOCACHE"
+
 
 curl -L "$OBIURL4" > master.zip
 unzip master.zip
@@ -112,11 +124,12 @@ unzip master.zip
 echo "Install OBITOOLS from : $OBIURL4"
 
 cd obitools4-master || exit
+mkdir vendor
 
 if [[ -z "$OBITOOLS_PREFIX" ]] ; then
-  make
+  make GOFLAGS="-buildvcs=false" 
 else
-  make OBITOOLS_PREFIX="${OBITOOLS_PREFIX}"
+  make GOFLAGS="-buildvcs=false" OBITOOLS_PREFIX="${OBITOOLS_PREFIX}"
 fi
 
 (cp build/* "${INSTALL_DIR}/bin" 2> /dev/null) \
@@ -125,5 +138,6 @@ fi
 
 popd || exit
 
+chmod -R +w "$WORK_DIR"
 rm -rf "$WORK_DIR"
 

kmer_roaring_index/FREQUENCY_FILTER_FINAL.md

@@ -0,0 +1,292 @@
+# Filtre de Fréquence avec v Niveaux de Roaring Bitmaps
+
+## Algorithme
+
+```go
+Pour chaque k-mer rencontré dans les données:
+    c = 0
+    tant que (k-mer ∈ index[c] ET c < v):
+        c++
+    
+    si c < v:
+        index[c].insert(k-mer)
+```
+
+**Résultat** : `index[v-1]` contient les k-mers vus **≥ v fois**
+
+---
+
+## Exemple d'exécution (v=3)
+
+```
+Données:
+  Read1: kmer X
+  Read2: kmer X
+  Read3: kmer X  (X vu 3 fois)
+  Read4: kmer Y
+  Read5: kmer Y  (Y vu 2 fois)
+  Read6: kmer Z  (Z vu 1 fois)
+
+Exécution:
+
+Read1 (X):
+  c=0: X ∉ index[0] → index[0].add(X)
+  État: index[0]={X}, index[1]={}, index[2]={}
+
+Read2 (X):
+  c=0: X ∈ index[0] → c=1
+  c=1: X ∉ index[1] → index[1].add(X)
+  État: index[0]={X}, index[1]={X}, index[2]={}
+
+Read3 (X):
+  c=0: X ∈ index[0] → c=1
+  c=1: X ∈ index[1] → c=2
+  c=2: X ∉ index[2] → index[2].add(X)
+  État: index[0]={X}, index[1]={X}, index[2]={X}
+
+Read4 (Y):
+  c=0: Y ∉ index[0] → index[0].add(Y)
+  État: index[0]={X,Y}, index[1]={X}, index[2]={X}
+
+Read5 (Y):
+  c=0: Y ∈ index[0] → c=1
+  c=1: Y ∉ index[1] → index[1].add(Y)
+  État: index[0]={X,Y}, index[1]={X,Y}, index[2]={X}
+
+Read6 (Z):
+  c=0: Z ∉ index[0] → index[0].add(Z)
+  État: index[0]={X,Y,Z}, index[1]={X,Y}, index[2]={X}
+
+Résultat final:
+  index[0] (freq≥1): {X, Y, Z}
+  index[1] (freq≥2): {X, Y}
+  index[2] (freq≥3): {X}  ← K-mers filtrés ✓
+```
+
+---
+
+## Utilisation
+
+```go
+// Créer le filtre
+filter := obikmer.NewFrequencyFilter(31, 3) // k=31, minFreq=3
+
+// Ajouter les séquences
+for _, read := range reads {
+    filter.AddSequence(read)
+}
+
+// Récupérer les k-mers filtrés (freq ≥ 3)
+filtered := filter.GetFilteredSet("filtered")
+fmt.Printf("K-mers de qualité: %d\n", filtered.Cardinality())
+
+// Statistiques
+stats := filter.Stats()
+fmt.Println(stats.String())
+```
+
+---
+
+## Performance
+
+### Complexité
+
+**Par k-mer** :
+- Lookups : Moyenne ~v/2, pire cas v
+- Insertions : 1 Add
+- **Pas de Remove** ✅
+
+**Total pour n k-mers** :
+- Temps : O(n × v/2)
+- Mémoire : O(unique_kmers × v × 2 bytes)
+
+### Early exit pour distribution skewed
+
+Avec distribution typique (séquençage) :
+```
+80% singletons → 1 lookup (early exit)
+15% freq 2-3   → 2-3 lookups
+5% freq ≥4     → jusqu'à v lookups
+
+Moyenne réelle : ~2 lookups/kmer (au lieu de v/2)
+```
+
+---
+
+## Mémoire
+
+### Pour 10^8 k-mers uniques
+
+| v (minFreq) | Nombre bitmaps | Mémoire | vs map simple |
+|-------------|----------------|---------|---------------|
+| v=2 | 2 | ~400 MB | 6x moins |
+| v=3 | 3 | ~600 MB | 4x moins |
+| v=5 | 5 | ~1 GB | 2.4x moins |
+| v=10 | 10 | ~2 GB | 1.2x moins |
+| v=20 | 20 | ~4 GB | ~égal |
+
+**Note** : Avec distribution skewed (beaucoup de singletons), la mémoire réelle est bien plus faible car les niveaux hauts ont peu d'éléments.
+
+### Exemple réaliste (séquençage)
+
+Pour 10^8 k-mers totaux, v=3 :
+```
+Distribution:
+  80% singletons  → 80M dans index[0]
+  15% freq 2-3    → 15M dans index[1]
+  5% freq ≥3      → 5M dans index[2]
+
+Mémoire:
+  index[0]: 80M × 2 bytes = 160 MB
+  index[1]: 15M × 2 bytes = 30 MB
+  index[2]: 5M × 2 bytes = 10 MB
+  Total: ~200 MB ✅
+
+vs map simple: 80M × 24 bytes = ~2 GB
+Réduction: 10x
+```
+
+---
+
+## Comparaison des approches
+
+| Approche | Mémoire (10^8 kmers) | Passes | Lookups/kmer | Quand utiliser |
+|----------|----------------------|--------|--------------|----------------|
+| **v-Bitmaps** | **200-600 MB** | **1** | **~2 (avg)** | **Standard** ✅ |
+| Map simple | 2.4 GB | 1 | 1 | Si RAM illimitée |
+| Multi-pass | 400 MB | v | v | Si I/O pas cher |
+
+---
+
+## Avantages de v-Bitmaps
+
+✅ **Une seule passe** sur les données  
+✅ **Mémoire optimale** avec Roaring bitmaps  
+✅ **Pas de Remove** (seulement Contains + Add)  
+✅ **Early exit** efficace sur singletons  
+✅ **Scalable** jusqu'à v~10-20  
+✅ **Simple** à implémenter et comprendre  
+
+---
+
+## Cas d'usage typiques
+
+### 1. Éliminer erreurs de séquençage
+
+```go
+filter := obikmer.NewFrequencyFilter(31, 3)
+
+// Traiter FASTQ
+for read := range StreamFastq("sample.fastq") {
+    filter.AddSequence(read)
+}
+
+// K-mers de qualité (pas d'erreurs)
+cleaned := filter.GetFilteredSet("cleaned")
+```
+
+**Résultat** : Élimine 70-80% des k-mers (erreurs)
+
+### 2. Assemblage de génome
+
+```go
+filter := obikmer.NewFrequencyFilter(31, 2)
+
+// Filtrer avant l'assemblage
+for read := range reads {
+    filter.AddSequence(read)
+}
+
+solidKmers := filter.GetFilteredSet("solid")
+// Utiliser solidKmers pour le graphe de Bruijn
+```
+
+### 3. Comparaison de génomes
+
+```go
+collection := obikmer.NewKmerSetCollection(31)
+
+for _, genome := range genomes {
+    filter := obikmer.NewFrequencyFilter(31, 3)
+    filter.AddSequences(genome.Reads)
+    
+    cleaned := filter.GetFilteredSet(genome.ID)
+    collection.Add(cleaned)
+}
+
+// Analyses comparatives sur k-mers de qualité
+matrix := collection.ParallelPairwiseJaccard(8)
+```
+
+---
+
+## Limites
+
+**Pour v > 20** :
+- Trop de lookups (v lookups/kmer)
+- Mémoire importante (v × 200MB pour 10^8 kmers)
+
+**Solutions alternatives pour v > 20** :
+- Utiliser map simple (9 bytes/kmer) si RAM disponible
+- Algorithme différent (sketch, probabiliste)
+
+---
+
+## Optimisations possibles
+
+### 1. Parallélisation
+
+```go
+// Traiter plusieurs fichiers en parallèle
+filters := make([]*FrequencyFilter, numFiles)
+
+var wg sync.WaitGroup
+for i, file := range files {
+    wg.Add(1)
+    go func(idx int, f string) {
+        defer wg.Done()
+        filters[idx] = ProcessFile(f, k, minFreq)
+    }(i, file)
+}
+wg.Wait()
+
+// Merger les résultats
+merged := MergeFilters(filters)
+```
+
+### 2. Streaming avec seuil adaptatif
+
+```go
+// Commencer avec v=5, réduire progressivement
+filter := obikmer.NewFrequencyFilter(31, 5)
+
+// ... traitement ...
+
+// Si trop de mémoire, réduire à v=3
+if filter.MemoryUsage() > threshold {
+    filter = ConvertToLowerThreshold(filter, 3)
+}
+```
+
+---
+
+## Récapitulatif final
+
+**Pour filtrer les k-mers par fréquence ≥ v :**
+
+1. **Créer** : `filter := NewFrequencyFilter(k, v)`
+2. **Traiter** : `filter.AddSequence(read)` pour chaque read
+3. **Résultat** : `filtered := filter.GetFilteredSet(id)`
+
+**Mémoire** : ~2v MB par million de k-mers uniques  
+**Temps** : Une seule passe, ~2 lookups/kmer en moyenne  
+**Optimal pour** : v ≤ 20, distribution skewed (séquençage)  
+
+---
+
+## Code fourni
+
+1. **frequency_filter.go** - Implémentation complète
+2. **examples_frequency_filter_final.go** - Exemples d'utilisation
+
+**Tout est prêt à utiliser !** 🚀

kmer_roaring_index/examples_frequency_filter_final.go

@@ -0,0 +1,320 @@
+package main
+
+import (
+	"fmt"
+	"obikmer"
+)
+
+func main() {
+	// ==========================================
+	// EXEMPLE 1 : Utilisation basique
+	// ==========================================
+	fmt.Println("=== EXEMPLE 1 : Utilisation basique ===\n")
+
+	k := 31
+	minFreq := 3 // Garder les k-mers vus ≥3 fois
+
+	// Créer le filtre
+	filter := obikmer.NewFrequencyFilter(k, minFreq)
+
+	// Simuler des séquences avec différentes fréquences
+	sequences := [][]byte{
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"), // Kmer X
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"), // Kmer X (freq=2)
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"), // Kmer X (freq=3) ✓
+		[]byte("TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT"), // Kmer Y
+		[]byte("TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT"), // Kmer Y (freq=2) ✗
+		[]byte("GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG"), // Kmer Z (freq=1) ✗
+	}
+
+	fmt.Printf("Traitement de %d séquences...\n", len(sequences))
+	for _, seq := range sequences {
+		filter.AddSequence(seq)
+	}
+
+	// Récupérer les k-mers filtrés
+	filtered := filter.GetFilteredSet("filtered")
+	fmt.Printf("\nK-mers avec freq ≥ %d: %d\n", minFreq, filtered.Cardinality())
+
+	// Statistiques
+	stats := filter.Stats()
+	fmt.Println("\n" + stats.String())
+
+	// ==========================================
+	// EXEMPLE 2 : Vérifier les niveaux
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 2 : Inspection des niveaux ===\n")
+
+	// Vérifier chaque niveau
+	for level := 0; level < minFreq; level++ {
+		levelSet := filter.GetKmersAtLevel(level)
+		fmt.Printf("Niveau %d (freq≥%d): %d k-mers\n",
+			level+1, level+1, levelSet.Cardinality())
+	}
+
+	// ==========================================
+	// EXEMPLE 3 : Données réalistes
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 3 : Simulation données séquençage ===\n")
+
+	filter2 := obikmer.NewFrequencyFilter(31, 3)
+
+	// Simuler un dataset réaliste :
+	// - 1000 reads
+	// - 80% contiennent des erreurs (singletons)
+	// - 15% vrais k-mers à basse fréquence
+	// - 5% vrais k-mers à haute fréquence
+
+	// Vraie séquence répétée
+	trueSeq := []byte("ACGTACGTACGTACGTACGTACGTACGTACG")
+	for i := 0; i < 50; i++ {
+		filter2.AddSequence(trueSeq)
+	}
+
+	// Séquence à fréquence moyenne
+	mediumSeq := []byte("CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC")
+	for i := 0; i < 5; i++ {
+		filter2.AddSequence(mediumSeq)
+	}
+
+	// Erreurs de séquençage (singletons)
+	for i := 0; i < 100; i++ {
+		errorSeq := []byte(fmt.Sprintf("TTTTTTTTTTTTTTTTTTTTTTTTTTTT%03d", i))
+		filter2.AddSequence(errorSeq)
+	}
+
+	stats2 := filter2.Stats()
+	fmt.Println(stats2.String())
+
+	fmt.Println("Distribution attendue:")
+	fmt.Println("  - Beaucoup de singletons (erreurs)")
+	fmt.Println("  - Peu de k-mers à haute fréquence (signal)")
+	fmt.Println("  → Filtrage efficace !")
+
+	// ==========================================
+	// EXEMPLE 4 : Tester différents seuils
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 4 : Comparaison de seuils ===\n")
+
+	testSeqs := [][]byte{
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+		[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"), // freq=5
+		[]byte("TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT"),
+		[]byte("TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT"),
+		[]byte("TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT"), // freq=3
+		[]byte("GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG"), // freq=1
+	}
+
+	for _, minFreq := range []int{2, 3, 5} {
+		f := obikmer.NewFrequencyFilter(31, minFreq)
+		f.AddSequences(testSeqs)
+
+		fmt.Printf("minFreq=%d: %d k-mers retenus (%.2f MB)\n",
+			minFreq,
+			f.Cardinality(),
+			float64(f.MemoryUsage())/1024/1024)
+	}
+
+	// ==========================================
+	// EXEMPLE 5 : Comparaison mémoire
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 5 : Comparaison mémoire ===\n")
+
+	filter3 := obikmer.NewFrequencyFilter(31, 3)
+
+	// Simuler 10000 séquences
+	for i := 0; i < 10000; i++ {
+		seq := make([]byte, 100)
+		for j := range seq {
+			seq[j] = "ACGT"[(i+j)%4]
+		}
+		filter3.AddSequence(seq)
+	}
+
+	fmt.Println(filter3.CompareWithSimpleMap())
+
+	// ==========================================
+	// EXEMPLE 6 : Workflow complet
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 6 : Workflow complet ===\n")
+
+	fmt.Println("1. Créer le filtre")
+	finalFilter := obikmer.NewFrequencyFilter(31, 3)
+
+	fmt.Println("2. Traiter les données (simulation)")
+	// En pratique : lire depuis FASTQ
+	// for read := range ReadFastq("data.fastq") {
+	//     finalFilter.AddSequence(read)
+	// }
+
+	// Simulation
+	for i := 0; i < 1000; i++ {
+		seq := []byte("ACGTACGTACGTACGTACGTACGTACGTACG")
+		finalFilter.AddSequence(seq)
+	}
+
+	fmt.Println("3. Récupérer les k-mers filtrés")
+	result := finalFilter.GetFilteredSet("final")
+
+	fmt.Println("4. Utiliser le résultat")
+	fmt.Printf("   K-mers de qualité: %d\n", result.Cardinality())
+	fmt.Printf("   Mémoire utilisée: %.2f MB\n", float64(finalFilter.MemoryUsage())/1024/1024)
+
+	fmt.Println("5. Sauvegarder (optionnel)")
+	// result.Save("filtered_kmers.bin")
+
+	// ==========================================
+	// EXEMPLE 7 : Vérification individuelle
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 7 : Vérification de k-mers spécifiques ===\n")
+
+	checkFilter := obikmer.NewFrequencyFilter(31, 3)
+
+	testSeq := []byte("ACGTACGTACGTACGTACGTACGTACGTACG")
+	for i := 0; i < 5; i++ {
+		checkFilter.AddSequence(testSeq)
+	}
+
+	var kmers []uint64
+	kmers = obikmer.EncodeKmers(testSeq, 31, &kmers)
+
+	if len(kmers) > 0 {
+		testKmer := kmers[0]
+
+		fmt.Printf("K-mer test: 0x%016X\n", testKmer)
+		fmt.Printf("  Présent dans filtre: %v\n", checkFilter.Contains(testKmer))
+		fmt.Printf("  Fréquence approx: %d\n", checkFilter.GetFrequency(testKmer))
+	}
+
+	// ==========================================
+	// EXEMPLE 8 : Intégration avec collection
+	// ==========================================
+	fmt.Println("\n=== EXEMPLE 8 : Intégration avec KmerSetCollection ===\n")
+
+	// Créer une collection de génomes filtrés
+	collection := obikmer.NewKmerSetCollection(31)
+
+	genomes := map[string][][]byte{
+		"Genome1": {
+			[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+			[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+			[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+			[]byte("TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT"), // Erreur
+		},
+		"Genome2": {
+			[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+			[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+			[]byte("ACGTACGTACGTACGTACGTACGTACGTACG"),
+			[]byte("GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG"), // Erreur
+		},
+	}
+
+	for id, sequences := range genomes {
+		// Filtrer chaque génome
+		genomeFilter := obikmer.NewFrequencyFilter(31, 3)
+		genomeFilter.AddSequences(sequences)
+
+		// Ajouter à la collection
+		filteredSet := genomeFilter.GetFilteredSet(id)
+		collection.Add(filteredSet)
+
+		fmt.Printf("%s: %d k-mers de qualité\n", id, filteredSet.Cardinality())
+	}
+
+	// Analyser la collection
+	fmt.Println("\nAnalyse comparative:")
+	collectionStats := collection.ComputeStats()
+	fmt.Printf("  Core genome: %d k-mers\n", collectionStats.CoreSize)
+	fmt.Printf("  Pan genome: %d k-mers\n", collectionStats.PanGenomeSize)
+
+	// ==========================================
+	// RÉSUMÉ
+	// ==========================================
+	fmt.Println("\n=== RÉSUMÉ ===\n")
+	fmt.Println("Le FrequencyFilter permet de:")
+	fmt.Println("  ✓ Filtrer les k-mers par fréquence minimale")
+	fmt.Println("  ✓ Utiliser une mémoire optimale avec Roaring bitmaps")
+	fmt.Println("  ✓ Une seule passe sur les données")
+	fmt.Println("  ✓ Éliminer efficacement les erreurs de séquençage")
+	fmt.Println("")
+	fmt.Println("Workflow typique:")
+	fmt.Println("  1. filter := NewFrequencyFilter(k, minFreq)")
+	fmt.Println("  2. for each sequence: filter.AddSequence(seq)")
+	fmt.Println("  3. filtered := filter.GetFilteredSet(id)")
+	fmt.Println("  4. Utiliser filtered dans vos analyses")
+}
+
+// ==================================
+// FONCTION HELPER POUR BENCHMARKS
+// ==================================
+
+func BenchmarkFrequencyFilter() {
+	k := 31
+	minFreq := 3
+
+	// Test avec différentes tailles
+	sizes := []int{1000, 10000, 100000}
+
+	fmt.Println("\n=== BENCHMARK ===\n")
+
+	for _, size := range sizes {
+		filter := obikmer.NewFrequencyFilter(k, minFreq)
+
+		// Générer des séquences
+		for i := 0; i < size; i++ {
+			seq := make([]byte, 100)
+			for j := range seq {
+				seq[j] = "ACGT"[(i+j)%4]
+			}
+			filter.AddSequence(seq)
+		}
+
+		fmt.Printf("Size=%d reads:\n", size)
+		fmt.Printf("  Filtered k-mers: %d\n", filter.Cardinality())
+		fmt.Printf("  Memory: %.2f MB\n", float64(filter.MemoryUsage())/1024/1024)
+		fmt.Println()
+	}
+}
+
+// ==================================
+// FONCTION POUR DONNÉES RÉELLES
+// ==================================
+
+func ProcessRealData() {
+	// Exemple pour traiter de vraies données FASTQ
+
+	k := 31
+	minFreq := 3
+
+	filter := obikmer.NewFrequencyFilter(k, minFreq)
+
+	// Pseudo-code pour lire un FASTQ
+	/*
+	fastqFile := "sample.fastq"
+	reader := NewFastqReader(fastqFile)
+
+	for reader.HasNext() {
+		read := reader.Next()
+		filter.AddSequence(read.Sequence)
+	}
+
+	// Récupérer le résultat
+	filtered := filter.GetFilteredSet("sample_filtered")
+	filtered.Save("sample_filtered_kmers.bin")
+
+	// Stats
+	stats := filter.Stats()
+	fmt.Println(stats.String())
+	*/
+
+	fmt.Println("Workflow pour données réelles:")
+	fmt.Println("  1. Créer le filtre avec minFreq approprié (2-5 typique)")
+	fmt.Println("  2. Stream les reads depuis FASTQ")
+	fmt.Println("  3. Récupérer les k-mers filtrés")
+	fmt.Println("  4. Utiliser pour assemblage/comparaison/etc.")
+
+	_ = filter // unused
+}

obitests/obitools/obiannotate/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obiannotate
+CMD=obiannotate
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obiclean/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obiclean
+CMD=obiclean
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obicleandb/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obicleandb
+CMD=obicleandb
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obicomplement/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obicomplement
+CMD=obicomplement
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obiconsensus/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obiconsensus
+CMD=obiconsensus
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obiconvert/test.sh

@@ -0,0 +1,144 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obiconvert
+CMD=obiconvert
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+
+if [ -z "$TEST_DIR" ] ; then
+   TEST_DIR="."
+fi
+
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+((ntest++))
+if obiconvert -Z "${TEST_DIR}/gbpln1088.4Mb.fasta.gz" \
+                 > "${TMPDIR}/xxx.fasta.gz" && \
+   zdiff "${TEST_DIR}/gbpln1088.4Mb.fasta.gz" \
+                 "${TMPDIR}/xxx.fasta.gz" 
+then
+    log "$MCMD: converting large fasta file to fasta OK" 
+    ((success++))
+else
+    log "$MCMD: converting large fasta file to fasta failed" 
+    ((failed++))
+fi
+ 
+((ntest++))
+if obiconvert -Z --fastq-output \
+              "${TEST_DIR}/gbpln1088.4Mb.fasta.gz" \
+                 > "${TMPDIR}/xxx.fastq.gz" && \
+   obiconvert -Z --fasta-output \
+              "${TMPDIR}/xxx.fastq.gz" \
+              > "${TMPDIR}/yyy.fasta.gz" && \
+   zdiff "${TEST_DIR}/gbpln1088.4Mb.fasta.gz" \
+                 "${TMPDIR}/yyy.fasta.gz" 
+then
+    log "$MCMD: converting large file between fasta and fastq OK" 
+    ((success++))
+else
+    log "$MCMD: converting large file between fasta and fastq failed" 
+    ((failed++))
+fi
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obicount/test.sh

@@ -5,6 +5,7 @@
 #
 
 TEST_NAME=obicount
+CMD=obicount
 
 ######
 #
@@ -15,6 +16,7 @@ TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
 OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
 export PATH="${OBITOOLS_DIR}:${PATH}"
 
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
 
 TMPDIR="$(mktemp -d)"
 ntest=0
@@ -38,9 +40,14 @@ cleanup() {
 
     if [ $failed -gt 0 ]; then
        log "$TEST_NAME tests failed" 
+        log
+        log
        exit 1
     fi
 
+    log
+    log
+
     exit 0
 }
 
@@ -79,6 +86,18 @@ log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
 ####
 ######################################################################
 
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
 ((ntest++))
 if obicount "${TEST_DIR}/wolf_F.fasta.gz" \
     > "${TMPDIR}/wolf_F.fasta_count.csv" 

obitests/obitools/obicsv/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obicsv
+CMD=obicsv
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obidemerge/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obidemerge
+CMD=obidemerge
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obidistribute/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obidistribute
+CMD=obidistribute
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obigrep/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obigrep
+CMD=obigrep
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obijoin/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obijoin
+CMD=obijoin
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obikmermatch/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obikmermatch
+CMD=obikmermatch
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obikmersimcount/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obikmersimcount
+CMD=obikmersimcount
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obilandmark/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obilandmark
+CMD=obilandmark
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obimatrix/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obimatrix
+CMD=obimatrix
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obimicrosat/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obimicrosat
+CMD=obimicrosat
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obimultiplex/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obimultiplex
+CMD=obimultiplex
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obipairing/test.sh

@@ -4,7 +4,8 @@
 # Here give the name of the test serie
 #
 
-TEST_NAME=obiparing
+TEST_NAME=obipairing
+CMD=obipairing
 
 ######
 #
@@ -15,6 +16,7 @@ TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
 OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
 export PATH="${OBITOOLS_DIR}:${PATH}"
 
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
 
 TMPDIR="$(mktemp -d)"
 ntest=0
@@ -38,9 +40,13 @@ cleanup() {
 
     if [ $failed -gt 0 ]; then
        log "$TEST_NAME tests failed" 
+       log
+       log
        exit 1
     fi
 
+    log
+    log
     exit 0
 }
 
@@ -79,6 +85,16 @@ log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
 ####
 ######################################################################
 
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
 ((ntest++))
 if obipairing -F "${TEST_DIR}/wolf_F.fastq.gz" \
               -R "${TEST_DIR}/wolf_R.fastq.gz" \
@@ -94,8 +110,8 @@ fi
 
 ((ntest++))
 if obicsv -Z -s -i \
-          -k ali_dir -k ali_length -k paring_fast_count \
+          -k ali_dir -k ali_length -k pairing_fast_count \
-          -k paring_fast_overlap -k paring_fast_score \
+          -k pairing_fast_overlap -k pairing_fast_score \
           -k score -k score_norm -k seq_a_single \
           -k seq_b_single -k seq_ab_match \
           "${TMPDIR}/wolf_paired_alignment.fastq.gz" \

obitests/obitools/obipcr/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obipcr
+CMD=obipcr
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obirefidx/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obirefidx
+CMD=obirefidx
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obiscript/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obiscript
+CMD=obiscript
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obisplit/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obisplit
+CMD=obisplit
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obisummary/some_uniq_seq.fasta

@@ -0,0 +1,9 @@
+>Seq_1 {"count":2,"merged_sample":{"15a_F730814":1,"29a_F260619":1}}
+ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
+agctyaaaactcaaaggacttggcggtgctttataccctt
+>Seq_2 {"count":22,"merged_sample":{"15a_F730814":12,"29a_F260619":10}}
+ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcaat
+atcttaaaactcaaaggacttggcggtgctttataccctt
+>Seq_3 {"count":22,"merged_sample":{"15a_F730814":15,"29a_F260619":7}}
+ttagccctaaacacaagtaattaatataacaaaattattcgccagagtactaccggcgat
+agcttaaaactcaaaggacttggcggtgctttataccctt

obitests/obitools/obisummary/some_uniq_seq.json

@@ -0,0 +1,35 @@
+{
+  "annotations": {
+    "keys": {
+      "map": {
+        "merged_sample": 3
+      },
+      "scalar": {
+        "count": 3
+      }
+    },
+    "map_attributes": 1,
+    "scalar_attributes": 1,
+    "vector_attributes": 0
+  },
+  "count": {
+    "reads": 46,
+    "total_length": 300,
+    "variants": 3
+  },
+  "samples": {
+    "sample_count": 2,
+    "sample_stats": {
+      "15a_F730814": {
+        "reads": 28,
+        "singletons": 1,
+        "variants": 3
+      },
+      "29a_F260619": {
+        "reads": 18,
+        "singletons": 1,
+        "variants": 3
+      }
+    }
+  }
+}

obitests/obitools/obisummary/some_uniq_seq.yaml

@@ -0,0 +1,25 @@
+annotations:
+    keys:
+        map:
+            merged_sample: 3
+        scalar:
+            count: 3
+    map_attributes: 1
+    scalar_attributes: 1
+    vector_attributes: 0
+count:
+    reads: 46
+    total_length: 300
+    variants: 3
+samples:
+    sample_count: 2
+    sample_stats:
+        15a_F730814:
+            reads: 28
+            singletons: 1
+            variants: 3
+        29a_F260619:
+            reads: 18
+            singletons: 1
+            variants: 3
+

obitests/obitools/obisummary/test.sh

@@ -0,0 +1,152 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obisummary
+CMD=obisummary
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+((ntest++))
+if obisummary "${TEST_DIR}/some_uniq_seq.fasta" \
+              > "${TMPDIR}/some_uniq_seq.json"
+then
+    log "$MCMD: formating json execution OK" 
+    ((success++))
+else
+    log "$MCMD: formating json execution failed" 
+    ((failed++))
+fi
+
+((ntest++))
+if diff "${TEST_DIR}/some_uniq_seq.json" \
+        "${TMPDIR}/some_uniq_seq.json" > /dev/null
+then
+    log "$MCMD: formating json OK" 
+    ((success++))
+else
+    log "$MCMD: formating json failed" 
+    ((failed++))
+fi
+
+((ntest++))
+if obisummary --yaml "${TEST_DIR}/some_uniq_seq.fasta" \
+              > "${TMPDIR}/some_uniq_seq.yaml"
+then
+    log "$MCMD: formating yaml execution OK" 
+    ((success++))
+else
+    log "$MCMD: formating yaml execution failed" 
+    ((failed++))
+fi
+
+((ntest++))
+if diff "${TEST_DIR}/some_uniq_seq.yaml" \
+        "${TMPDIR}/some_uniq_seq.yaml" > /dev/null
+then
+    log "$MCMD: formating yaml OK" 
+    ((success++))
+else
+    log "$MCMD: formating yaml failed" 
+    ((failed++))
+fi
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obitag/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obitag
+CMD=obitag
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obitagpcr/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obipcr
+CMD=obipcr
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obitaxonomy/test.sh

@@ -0,0 +1,109 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obitaxonomy
+CMD=obitaxonomy
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obiuniq/test.sh

@@ -0,0 +1,205 @@
+#!/bin/bash
+
+#
+# Here give the name of the test serie
+#
+
+TEST_NAME=obiuniq
+CMD=obiuniq
+
+######
+#
+# Some variable and function definitions: please don't change them
+#
+######
+TEST_DIR="$(dirname "$(readlink -f "${BASH_SOURCE[0]}")")"
+OBITOOLS_DIR="${TEST_DIR/obitest*/}build"
+export PATH="${OBITOOLS_DIR}:${PATH}"
+
+MCMD="$(echo "${CMD:0:4}" | tr '[:lower:]' '[:upper:]')$(echo "${CMD:4}" | tr '[:upper:]' '[:lower:]')"
+
+TMPDIR="$(mktemp -d)"
+ntest=0
+success=0
+failed=0
+
+cleanup() {
+    echo "========================================" 1>&2
+    echo "## Results of the $TEST_NAME tests:" 1>&2
+
+    echo 1>&2
+    echo "- $ntest tests run" 1>&2
+    echo "- $success successfully completed" 1>&2
+    echo "- $failed failed tests" 1>&2
+    echo 1>&2
+    echo "Cleaning up the temporary directory..." 1>&2
+    echo 1>&2
+    echo "========================================" 1>&2
+
+    rm -rf "$TMPDIR"  # Suppress the temporary directory
+
+    if [ $failed -gt 0 ]; then
+       log "$TEST_NAME tests failed" 
+        log
+        log
+       exit 1
+    fi
+
+    log
+    log
+
+    exit 0
+}
+
+log() {
+    echo -e "[$TEST_NAME @ $(date)] $*" 1>&2
+}
+
+log "Testing $TEST_NAME..." 
+log "Test directory is $TEST_DIR" 
+log "obitools directory is $OBITOOLS_DIR" 
+log "Temporary directory is $TMPDIR" 
+log "files: $(find $TEST_DIR | awk -F'/' '{print $NF}' | tail -n +2)"
+
+######################################################################
+####
+#### Below are the tests
+####
+#### Before each test :
+####  - increment the variable ntest
+####
+#### Run the command as the condition of an if / then /else
+####  - The command must return 0 on success
+####  - The command must return an exit code different from 0 on failure
+####  - The datafiles are stored in the same directory than the test script
+####  - The test script directory is stored in the TEST_DIR variable
+####  - If result files have to be produced they must be stored
+####    in the temporary directory (TMPDIR variable)
+####
+#### then clause is executed on success of the command
+####  - Write a success message using the log function
+####  - increment the variable success
+####
+#### else clause is executed on failure of the command
+####  - Write a failure message using the log function
+####  - increment the variable failed
+####
+######################################################################
+
+
+
+((ntest++))
+if $CMD -h > "${TMPDIR}/help.txt" 2>&1 
+then
+    log "$MCMD: printing help OK" 
+    ((success++))
+else
+    log "$MCMD: printing help failed" 
+    ((failed++))
+fi
+
+((ntest++))
+if obiuniq "${TEST_DIR}/touniq.fasta" \
+    > "${TMPDIR}/touniq_u.fasta"
+then
+    log "OBIUniq simple: running OK" 
+    ((success++))
+else
+    log "OBIUniq simple: running failed"
+    ((failed++))
+fi
+
+obicsv -s --auto ${TEST_DIR}/touniq_u.fasta \
+| tail -n +2 \
+| sort \
+> "${TMPDIR}/touniq_u_ref.csv"
+
+obicsv -s --auto ${TMPDIR}/touniq_u.fasta \
+| tail -n +2 \
+| sort \
+> "${TMPDIR}/touniq_u.csv"
+
+((ntest++))
+if diff "${TMPDIR}/touniq_u_ref.csv" \
+        "${TMPDIR}/touniq_u.csv"  > /dev/null
+then
+    log "OBIUniq simple: result OK"
+    ((success++))
+else
+    log "OBIUniq simple: result failed"
+    ((failed++))
+fi
+
+((ntest++))
+if obiuniq -c a "${TEST_DIR}/touniq.fasta" \
+    > "${TMPDIR}/touniq_u_a.fasta"
+then
+    log "OBIUniq one category: running OK" 
+    ((success++))
+else
+    log "OBIUniq one category: running failed"
+    ((failed++))
+fi
+
+obicsv -s --auto ${TEST_DIR}/touniq_u_a.fasta \
+| tail -n +2 \
+| sort \
+> "${TMPDIR}/touniq_u_a_ref.csv"
+
+obicsv -s --auto ${TMPDIR}/touniq_u_a.fasta \
+| tail -n +2 \
+| sort \
+> "${TMPDIR}/touniq_u_a.csv"
+
+
+((ntest++))
+if diff "${TMPDIR}/touniq_u_a_ref.csv" \
+        "${TMPDIR}/touniq_u_a.csv"  > /dev/null
+then
+    log "OBIUniq one category: result OK"
+    ((success++))
+else
+    log "OBIUniq one category: result failed"
+    ((failed++))
+fi
+
+((ntest++))
+if obiuniq -c a -c b "${TEST_DIR}/touniq.fasta" \
+    > "${TMPDIR}/touniq_u_a_b.fasta"
+then
+    log "OBIUniq two categories: running OK" 
+    ((success++))
+else
+    log "OBIUniq two categories: running failed"
+    ((failed++))
+fi
+
+obicsv -s --auto ${TEST_DIR}/touniq_u_a_b.fasta \
+| tail -n +2 \
+| sort \
+> "${TMPDIR}/touniq_u_a_b_ref.csv"
+
+obicsv -s --auto ${TMPDIR}/touniq_u_a_b.fasta \
+| tail -n +2 \
+| sort \
+> "${TMPDIR}/touniq_u_a_b.csv"
+
+((ntest++))
+if diff "${TMPDIR}/touniq_u_a_b_ref.csv" \
+        "${TMPDIR}/touniq_u_a_b.csv"  > /dev/null
+then
+    log "OBIUniq two categories: result OK"
+    ((success++))
+else
+    log "OBIUniq two categories: result failed"
+    ((failed++))
+fi
+
+#########################################
+#
+# At the end of the tests
+# the cleanup function is called
+#
+#########################################
+
+cleanup

obitests/obitools/obiuniq/touniq.fasta

@@ -0,0 +1,16 @@
+>seq1 {"a":2, "b":4,"c":5}
+aaacccgggttt
+>seq2 {"a":3, "b":4,"c":5}
+aaacccgggttt
+>seq3 {"a":3, "b":5,"c":5}
+aaacccgggttt
+>seq4 {"a":3, "b":5,"c":6}
+aaacccgggttt
+>seq5 {"a":2, "b":4,"c":5}
+aaacccgggtttca
+>seq6 {"a":3, "b":4,"c":5}
+aaacccgggtttca
+>seq7 {"a":3, "b":5,"c":5}
+aaacccgggtttca
+>seq8 {"a":3, "b":5,"c":6}
+aaacccgggtttca

obitests/obitools/obiuniq/touniq_u.fasta

@@ -0,0 +1,4 @@
+>seq5 {"count":4}
+aaacccgggtttca
+>seq1 {"count":4}
+aaacccgggttt

obitests/obitools/obiuniq/touniq_u_a.fasta

@@ -0,0 +1,8 @@
+>seq5 {"a":2,"b":4,"c":5,"count":1}
+aaacccgggtttca
+>seq6 {"a":3,"count":3}
+aaacccgggtttca
+>seq1 {"a":2,"b":4,"c":5,"count":1}
+aaacccgggttt
+>seq2 {"a":3,"count":3}
+aaacccgggttt

obitests/obitools/obiuniq/touniq_u_a_b.fasta

@@ -0,0 +1,12 @@
+>seq5 {"a":2,"b":4,"c":5,"count":1}
+aaacccgggtttca
+>seq6 {"a":3,"b":4,"c":5,"count":1}
+aaacccgggtttca
+>seq7 {"a":3,"b":5,"count":2}
+aaacccgggtttca
+>seq1 {"a":2,"b":4,"c":5,"count":1}
+aaacccgggttt
+>seq2 {"a":3,"b":4,"c":5,"count":1}
+aaacccgggttt
+>seq3 {"a":3,"b":5,"count":2}
+aaacccgggttt

pkg/obialign/alignment.go

@@ -169,7 +169,7 @@ func BuildQualityConsensus(seqA, seqB *obiseq.BioSequence, path []int, statOnMis
 
 	right = len(*bufferQA) - right
 
-	// log.Warnf("BuildQualityConsensus: left = %d right = %d\n", left, right)
+	// obilog.Warnf("BuildQualityConsensus: left = %d right = %d\n", left, right)
 
 	for i, qA = range *bufferQA {
 		nA := (*bufferSA)[i]

pkg/obialign/dnamatrix.go

@@ -117,7 +117,7 @@ func _MatchScoreRatio(QF, QR byte) (float64, float64) {
 	term1 := _Logaddexp(qF, qR)
 	term2 := _Logdiffexp(term1, qF+qR)
 
-	// log.Warnf("MatchScoreRatio: %v, %v , %v, %v", QF, QR, term1, term2)
+	// obilog.Warnf("MatchScoreRatio: %v, %v , %v, %v", QF, QR, term1, term2)
 
 	match_logp := _Log1mexp(term2 + l3 - l4)
 	match_score := match_logp - _Log1mexp(match_logp)

pkg/obialign/fastlcsegf.go

@@ -4,33 +4,6 @@ import (
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
 )
 
-var _iupac = [26]byte{
-	//  a   b  c  d   e  f
-	1, 14, 2, 13, 0, 0,
-	//  g   h  i  j  k   l
-	4, 11, 0, 0, 12, 0,
-	//  m   n  o  p  q  r
-	3, 15, 0, 0, 0, 5,
-	//  s  t  u   v  w  x
-	6, 8, 8, 13, 9, 0,
-	//  y   z
-	10, 0,
-}
-
-func _samenuc(a, b byte) bool {
-	if (a >= 'A') && (a <= 'Z') {
-		a |= 32
-	}
-	if (b >= 'A') && (b <= 'Z') {
-		b |= 32
-	}
-
-	if (a >= 'a') && (a <= 'z') && (b >= 'a') && (b <= 'z') {
-		return (_iupac[a-'a'] & _iupac[b-'a']) > 0
-	}
-	return a == b
-}
-
 // FastLCSEGFScoreByte calculates the score of the Longest Common Subsequence (LCS) between two byte slices.
 //
 // The score is calculated using the following scoring matrix:
@@ -165,7 +138,7 @@ func FastLCSEGFScoreByte(bA, bB []byte, maxError int, endgapfree bool, buffer *[
 			default:
 				// We are in the middle of the matrix
 				Sdiag = _incpath(previous[x])
-				if _samenuc(bA[j-1], bB[i-1]) {
+				if obiseq.SameIUPACNuc(bA[j-1], bB[i-1]) {
 					Sdiag = _incscore(Sdiag)
 				}
 
@@ -265,7 +238,7 @@ func FastLCSEGFScoreByte(bA, bB []byte, maxError int, endgapfree bool, buffer *[
 				Sleft = _notavail
 			default:
 				Sdiag = _incpath(previous[x])
-				if _samenuc(bA[j-1], bB[i-1]) {
+				if obiseq.SameIUPACNuc(bA[j-1], bB[i-1]) {
 					Sdiag = _incscore(Sdiag)
 				}
 

pkg/obialign/locatepattern.go

@@ -1,6 +1,9 @@
 package obialign
 
-import log "github.com/sirupsen/logrus"
+import (
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
+	log "github.com/sirupsen/logrus"
+)
 
 // buffIndex converts a pair of coordinates (i, j) into a linear index in a matrix
 // of size width x width. The coordinates are (-1)-indexed, and the linear index
@@ -69,7 +72,7 @@ func LocatePattern(id string, pattern, sequence []byte) (int, int, int) {
 			// Mismatch score = -1
 			// Match score = 0
 			match := -1
-			if _samenuc(pattern[j], sequence[i]) {
+			if obiseq.SameIUPACNuc(pattern[j], sequence[i]) {
 				match = 0
 			}
 
@@ -103,7 +106,7 @@ func LocatePattern(id string, pattern, sequence []byte) (int, int, int) {
 		// Mismatch score = -1
 		// Match score = 0
 		match := -1
-		if _samenuc(pattern[jmax], sequence[i]) {
+		if obiseq.SameIUPACNuc(pattern[jmax], sequence[i]) {
 			match = 0
 		}
 
@@ -152,7 +155,7 @@ func LocatePattern(id string, pattern, sequence []byte) (int, int, int) {
 
 	}
 
-	// log.Warnf("from : %d to: %d error: %d match: %v",
+	// obilog.Warnf("from : %d to: %d error: %d match: %v",
 	// 	i, end+1, -buffer[buffIndex(len(sequence)-1, len(pattern)-1, width)],
 	// 	string(sequence[i:(end+1)]))
 	return i, end + 1, -buffer[buffIndex(len(sequence)-1, len(pattern)-1, width)]

pkg/obialign/readalign.go

@@ -53,10 +53,10 @@ func ReadAlign(seqA, seqB *obiseq.BioSequence,
 		over = min(seqA.Len(), seqB.Len())
 	}
 
-	// log.Warnf("fw/fw: %v shift=%d fastCount=%d/over=%d fastScore=%f",
+	// obilog.Warnf("fw/fw: %v shift=%d fastCount=%d/over=%d fastScore=%f",
 	// 	directAlignment, shift, fastCount, over, fastScore)
 
-	// log.Warnf(("seqA: %s\nseqB: %s\n"), seqA.String(), seqB.String())
+	// obilog.Warnf(("seqA: %s\nseqB: %s\n"), seqA.String(), seqB.String())
 
 	// At least one mismatch exists in the overlaping region
 	if fastCount+3 < over {

pkg/obiapat/obiapat.c

@@ -149,9 +149,9 @@ char *LowerSequence(char *seq)
         char *cseq;
 
         for (cseq = seq ; *cseq ; cseq++) 
-            if (IS_UPPER(*cseq))
+            if (IS_UPPER(*cseq)) {
                 *cseq = TO_LOWER(*cseq);
-
+			}
 		return seq;
 }
  
@@ -299,14 +299,14 @@ int32_t delete_apatseq(SeqPtr pseq,
         return 1;
 }
 
-PatternPtr buildPattern(const char *pat, int32_t error_max, uint8_t hasIndel, 
+Pattern *buildPattern(const char *pat, int32_t error_max, uint8_t hasIndel, 
 						int *errno, char **errmsg)
 {
-	PatternPtr pattern;
+	Pattern *pattern;
 	int32_t    patlen;
 	int32_t    patlen2;
 
-	patlen  = strlen(pat);
+	patlen  = (int32_t)strlen(pat);
 	patlen2 = lenPattern(pat);
 
 	pattern = ECOMALLOC(sizeof(Pattern) +                   // Space for struct Pattern
@@ -341,10 +341,10 @@ PatternPtr buildPattern(const char *pat, int32_t error_max, uint8_t hasIndel,
 		
 }
 
-PatternPtr complementPattern(PatternPtr pat, int *errno, 
+Pattern *complementPattern(Pattern *pat, int *errno, 
 								char **errmsg)
 {
-	PatternPtr pattern;
+	Pattern *pattern;
 	
 	pattern = ECOMALLOC(sizeof(Pattern) + 
 							sizeof(char)      * strlen(pat->cpat) + 1 +

pkg/obiapat/obiapat.h

@@ -116,13 +116,13 @@ ecoseq_t *new_ecoseq_with_data( char *AC,
 
 
 
-int32_t  delete_apatseq(SeqPtr pseq, 
+int32_t  delete_apatseq(Seq *pseq, 
 				   int *errno, char **errmsg);
-PatternPtr buildPattern(const char *pat, int32_t error_max, uint8_t hasIndel, int *errno, char **errmsg);
+Pattern *buildPattern(const char *pat, int32_t error_max, uint8_t hasIndel, int *errno, char **errmsg);
-PatternPtr complementPattern(PatternPtr pat, int *errno, char **errmsg);
+Pattern *complementPattern(Pattern *pat, int *errno, char **errmsg);
 
-SeqPtr new_apatseq(const char *in,int32_t circular, int32_t seqlen,
+Seq *new_apatseq(const char *in,int32_t circular, int32_t seqlen,
-                    SeqPtr out, 
+                    Seq *out, 
 					int *errno, char **errmsg);
 					
 char *ecoComplementPattern(char *nucAcSeq);

pkg/obiapat/pattern.go

@@ -26,7 +26,7 @@ var _AllocatedApaPattern = 0
 // ApatPattern stores a regular pattern usable by the
 // Apat algorithm functions and methods
 type _ApatPattern struct {
-	pointer *C.Pattern
+	pointer C.PatternPtr
 	pattern string
 }
 
@@ -72,6 +72,7 @@ var NilApatSequence = ApatSequence{nil}
 //
 // Returns an ApatPattern object and an error if the pattern is invalid.
 func MakeApatPattern(pattern string, errormax int, allowsIndel bool) (ApatPattern, error) {
+
 	cpattern := C.CString(pattern)
 	defer C.free(unsafe.Pointer(cpattern))
 	cerrormax := C.int32_t(errormax)
@@ -159,7 +160,7 @@ func (pattern ApatPattern) Free() {
 // Print method prints the ApatPattern to the standard output.
 // This is mainly a debug method.
 func (pattern ApatPattern) Print() {
-	C.PrintDebugPattern(C.PatternPtr(pattern.pointer.pointer))
+	C.PrintDebugPattern((*C.Pattern)(pattern.pointer.pointer))
 }
 
 // MakeApatSequence casts an obiseq.BioSequence to an ApatSequence.
@@ -410,8 +411,8 @@ func (pattern ApatPattern) FilterBestMatch(sequence ApatSequence, begin, length
 
 	best := [3]int{0, 0, 10000}
 	for _, m := range res {
-		// log.Warnf("Current : Begin : %d End : %d Err : %d", m[0], m[1], m[2])
+		// obilog.Warnf("Current : Begin : %d End : %d Err : %d", m[0], m[1], m[2])
-		// log.Warnf("Best    : Begin : %d End : %d Err : %d", best[0], best[1], best[2])
+		// obilog.Warnf("Best    : Begin : %d End : %d Err : %d", best[0], best[1], best[2])
 		if (m[0] - m[2]) < best[1]+best[2] {
 			// match are overlapping
 			// log.Warnln("overlap")
@@ -467,7 +468,7 @@ func (pattern ApatPattern) AllMatches(sequence ApatSequence, begin, length int)
 		// Recompute the start and end position of the match
 		// when the pattern allows for indels
 		if m[2] > 0 && pattern.pointer.pointer.hasIndel {
-			// log.Warnf("Locating indel on sequence %s[%s]", sequence.pointer.reference.Id(), pattern.String())
+			// obilog.Warnf("Locating indel on sequence %s[%s]", sequence.pointer.reference.Id(), pattern.String())
 			start := m[0] - m[2]*2
 			start = max(start, 0)
 			end := start + int(pattern.pointer.pointer.patlen) + 4*m[2]
@@ -489,7 +490,7 @@ func (pattern ApatPattern) AllMatches(sequence ApatSequence, begin, length int)
 			m[0] = start + pb
 			m[1] = start + pe
 
-			// log.Warnf("seq[%d@%d:%d] %d: %s %d - %s:%s:%s", i, m[0], m[1], olderr, sequence.pointer.reference.Id(), score,
+			// obilog.Warnf("seq[%d@%d:%d] %d: %s %d - %s:%s:%s", i, m[0], m[1], olderr, sequence.pointer.reference.Id(), score,
 			// 	frg, (*cpattern)[0:int(pattern.pointer.pointer.patlen)], sequence.pointer.reference.Sequence()[m[0]:m[1]])
 		}
 

pkg/obichunk/chunk.go

@@ -0,0 +1,22 @@
+package obichunk
+
+import (
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiiter"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
+)
+
+func ISequenceChunk(iterator obiiter.IBioSequence,
+	classifier *obiseq.BioSequenceClassifier,
+	onMemory bool,
+	dereplicate bool,
+	na string,
+	statsOn obiseq.StatsOnDescriptions,
+	uniqueClassifier *obiseq.BioSequenceClassifier,
+) (obiiter.IBioSequence, error) {
+
+	if onMemory {
+		return ISequenceChunkOnMemory(iterator, classifier)
+	} else {
+		return ISequenceChunkOnDisk(iterator, classifier, dereplicate, na, statsOn, uniqueClassifier)
+	}
+}

pkg/obichunk/chunk_on_disk.go

@@ -10,6 +10,7 @@ import (
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiformats"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiiter"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiutils"
 )
 
 // tempDir creates a temporary directory with a prefix "obiseq_chunks_"
@@ -73,7 +74,13 @@ func find(root, ext string) []string {
 // is removed. The function logs the number of batches created and the processing
 // status of each batch.
 func ISequenceChunkOnDisk(iterator obiiter.IBioSequence,
-	classifier *obiseq.BioSequenceClassifier) (obiiter.IBioSequence, error) {
+	classifier *obiseq.BioSequenceClassifier,
+	dereplicate bool,
+	na string,
+	statsOn obiseq.StatsOnDescriptions,
+	uniqueClassifier *obiseq.BioSequenceClassifier,
+) (obiiter.IBioSequence, error) {
+	obiutils.RegisterAPipe()
 	dir, err := tempDir()
 	if err != nil {
 		return obiiter.NilIBioSequence, err
@@ -86,7 +93,7 @@ func ISequenceChunkOnDisk(iterator obiiter.IBioSequence,
 	go func() {
 		defer func() {
 			os.RemoveAll(dir)
-			log.Debugln("Clear the cache directory")
+			obiutils.UnregisterPipe()
 		}()
 
 		newIter.Wait()
@@ -111,11 +118,45 @@ func ISequenceChunkOnDisk(iterator obiiter.IBioSequence,
 				panic(err)
 			}
 
-			source, chunk := iseq.Load()
+			if dereplicate {
+				u := make(map[string]*obiseq.BioSequence)
+				var source string
+				uniqueClassifier.Reset()
 
-			newIter.Push(obiiter.MakeBioSequenceBatch(source, order, chunk))
+				for iseq.Next() {
-			log.Infof("Start processing of batch %d/%d : %d sequences",
+					batch := iseq.Get()
-				order, nbatch, len(chunk))
+					source = batch.Source()
+
+					for _, seq := range batch.Slice() {
+						// Use composite key: sequence + categories
+						code := uniqueClassifier.Code(seq)
+						key := uniqueClassifier.Value(code)
+						prev, ok := u[key]
+						if ok {
+							prev.Merge(seq, na, true, statsOn)
+						} else {
+							u[key] = seq
+						}
+					}
+				}
+
+				chunk := obiseq.MakeBioSequenceSlice(len(u))
+				i := 0
+
+				for _, seq := range u {
+					chunk[i] = seq
+					i++
+				}
+
+				newIter.Push(obiiter.MakeBioSequenceBatch(source, order, chunk))
+
+			} else {
+				source, chunk := iseq.Load()
+
+				newIter.Push(obiiter.MakeBioSequenceBatch(source, order, chunk))
+				log.Infof("Start processing of batch %d/%d : %d sequences",
+					order+1, nbatch, len(chunk))
+			}
 
 		}
 

pkg/obichunk/chunks_on_memory.go

@@ -9,7 +9,20 @@ import (
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
 )
 
-func ISequenceChunk(iterator obiiter.IBioSequence,
+// ISequenceChunkOnMemory processes a sequence iterator by distributing the sequences
+// into chunks in memory. It uses a classifier to determine how to distribute
+// the sequences and returns a new iterator for the processed sequences.
+//
+// Parameters:
+//   - iterator: An iterator of biosequences to be processed.
+//   - classifier: A pointer to a BioSequenceClassifier used to classify the sequences
+//     during distribution.
+//
+// Returns:
+// An iterator of biosequences representing the processed chunks.
+//
+// The function operates asynchronously.
+func ISequenceChunkOnMemory(iterator obiiter.IBioSequence,
 	classifier *obiseq.BioSequenceClassifier) (obiiter.IBioSequence, error) {
 
 	newIter := obiiter.MakeIBioSequence()

pkg/obichunk/unique.go

@@ -25,18 +25,35 @@ func IUniqueSequence(iterator obiiter.IBioSequence,
 
 	log.Infoln("Starting data splitting")
 
+	cat := opts.Categories()
+	na := opts.NAValue()
+
+	// Classifier for bucketing: Hash only to control number of chunks
+	bucketClassifier := obiseq.HashClassifier(opts.BatchCount())
+
+	// Classifier for uniqueness: Sequence + categories
+	var uniqueClassifier *obiseq.BioSequenceClassifier
+	if len(cat) > 0 {
+		cls := make([]*obiseq.BioSequenceClassifier, len(cat)+1)
+		cls[0] = obiseq.SequenceClassifier()
+		for i, c := range cat {
+			cls[i+1] = obiseq.AnnotationClassifier(c, na)
+		}
+		uniqueClassifier = obiseq.CompositeClassifier(cls...)
+	} else {
+		uniqueClassifier = obiseq.SequenceClassifier()
+	}
+
 	if opts.SortOnDisk() {
 		nworkers = 1
-		iterator, err = ISequenceChunkOnDisk(iterator,
+		iterator, err = ISequenceChunkOnDisk(iterator, bucketClassifier, true, na, opts.StatsOn(), uniqueClassifier)
-			obiseq.HashClassifier(opts.BatchCount()))
 
 		if err != nil {
 			return obiiter.NilIBioSequence, err
 		}
 
 	} else {
-		iterator, err = ISequenceChunk(iterator,
+		iterator, err = ISequenceChunkOnMemory(iterator, bucketClassifier)
-			obiseq.HashClassifier(opts.BatchCount()))
 
 		if err != nil {
 			return obiiter.NilIBioSequence, err
@@ -63,63 +80,25 @@ func IUniqueSequence(iterator obiiter.IBioSequence,
 		return neworder
 	}
 
-	var ff func(obiiter.IBioSequence,
+	ff := func(input obiiter.IBioSequence,
-		*obiseq.BioSequenceClassifier,
+		classifier *obiseq.BioSequenceClassifier) {
-		int)
-
-	cat := opts.Categories()
-	na := opts.NAValue()
-
-	ff = func(input obiiter.IBioSequence,
-		classifier *obiseq.BioSequenceClassifier,
-		icat int) {
-		icat--
 		input, err = ISequenceSubChunk(input,
 			classifier,
 			1)
 
-		var next obiiter.IBioSequence
-		if icat >= 0 {
-			next = obiiter.MakeIBioSequence()
-
-			iUnique.Add(1)
-
-			go ff(next,
-				obiseq.AnnotationClassifier(cat[icat], na),
-				icat)
-		}
-
-		o := 0
 		for input.Next() {
 			batch := input.Get()
-
+			if !(opts.NoSingleton() && len(batch.Slice()) == 1 && batch.Slice()[0].Count() == 1) {
-			if icat < 0 || len(batch.Slice()) == 1 {
+				iUnique.Push(batch.Reorder(nextOrder()))
-				// No more sub classification of sequence or only a single sequence
-				if !(opts.NoSingleton() && len(batch.Slice()) == 1 && batch.Slice()[0].Count() == 1) {
-					iUnique.Push(batch.Reorder(nextOrder()))
-				}
-			} else {
-				// A new step of classification must du realized
-				next.Push(batch.Reorder(o))
-				o++
 			}
 		}
-
-		if icat >= 0 {
-			next.Close()
-		}
-
 		iUnique.Done()
 	}
 
 	for i := 0; i < nworkers-1; i++ {
-		go ff(iterator.Split(),
+		go ff(iterator.Split(), uniqueClassifier.Clone())
-			obiseq.SequenceClassifier(),
-			len(cat))
 	}
-	go ff(iterator,
+	go ff(iterator, uniqueClassifier)
-		obiseq.SequenceClassifier(),
-		len(cat))
 
 	iMerged := iUnique.IMergeSequenceBatch(opts.NAValue(),
 		opts.StatsOn(),

pkg/obicorazick/worker.go

@@ -2,21 +2,75 @@ package obicorazick
 
 import (
 	log "github.com/sirupsen/logrus"
-
+	"sync"
+	"os"
 	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obiseq"
+	"git.metabarcoding.org/obitools/obitools4/obitools4/pkg/obidefault"
 	"github.com/rrethy/ahocorasick"
+	"github.com/schollz/progressbar/v3"
 )
 
 func AhoCorazickWorker(slot string, patterns []string) obiseq.SeqWorker {
 
-	matcher := ahocorasick.CompileStrings(patterns)
+	sizebatch:=10000000
+	nmatcher := len(patterns) / sizebatch + 1
+	log.Infof("Building AhoCorasick %d matcher for %d patterns in slot %s", 
+			 nmatcher, len(patterns), slot)
+
+	if nmatcher == 0 {
+		log.Errorln("No patterns provided")
+	}
+	
+	matchers := make([]*ahocorasick.Matcher, nmatcher)
+	ieme := make(chan int) 
+	mutex := &sync.WaitGroup{}
+	npar := min(obidefault.ParallelWorkers(), nmatcher)
+	mutex.Add(npar)
+
+	pbopt := make([]progressbar.Option, 0, 5)
+	pbopt = append(pbopt,
+		progressbar.OptionSetWriter(os.Stderr),
+		progressbar.OptionSetWidth(15),
+		progressbar.OptionShowCount(),
+		progressbar.OptionShowIts(),
+		progressbar.OptionSetDescription("Building AhoCorasick matcher..."),
+	)
+
+	bar := progressbar.NewOptions(nmatcher, pbopt...)
+	bar.Add(0)
+
+	builder := func() {
+		for i := range ieme  {		
+		matchers[i] = ahocorasick.CompileStrings(patterns[i*sizebatch:min((i+1)*sizebatch,len(patterns))])
+		bar.Add(1)
+		}
+		mutex.Done()
+	}
+
+	for i := 0; i < npar; i++ {
+		go builder()
+	}
+
+	for i := 0; i < nmatcher; i++ {
+		ieme <- i
+	}
+
+	close(ieme)
+	mutex.Wait()
 
 	fslot := slot + "_Fwd"
 	rslot := slot + "_Rev"
 
 	f := func(s *obiseq.BioSequence) (obiseq.BioSequenceSlice, error) {
-		matchesF := len(matcher.FindAllByteSlice(s.Sequence()))
+		matchesF := 0
-		matchesR := len(matcher.FindAllByteSlice(s.ReverseComplement(false).Sequence()))
+		matchesR := 0
+		b := s.Sequence()
+		bc := s.ReverseComplement(false).Sequence()
+
+		for _, matcher := range matchers {
+			matchesF += len(matcher.FindAllByteSlice(b))
+			matchesR += len(matcher.FindAllByteSlice(bc))
+		}
 
 		log.Debugln("Macthes = ", matchesF, matchesR)
 		matches := matchesF + matchesR

pkg/obidefault/compressed.go

@@ -1,15 +1,15 @@
 package obidefault
 
-var __compressed__ = false
+var __compress__ = false
 
 func CompressOutput() bool {
-	return __compressed__
+	return __compress__
 }
 
 func SetCompressOutput(b bool) {
-	__compressed__ = b
+	__compress__ = b
 }
 
-func CompressedPtr() *bool {
+func CompressOutputPtr() *bool {
-	return &__compressed__
+	return &__compress__
 }