Change the notation algorithm to take advantage of the new CAU tRNA
reference library Former-commit-id: 32650f41c4a7f95ce5da78c1f520438b35c1d4d1 Former-commit-id: 7ee31aaed2aca437b689fc7930095279fce0051b
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@ -54,40 +54,76 @@ function epissage(intron,seq) {
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}
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function patchtRNA(anticodon,trna,seq) {
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delete res;
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delete maxi;
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delete score;
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delete field;
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delete f2;
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if (anticodon == "cat") {
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file=printfasta(trna "_" anticodon,seq,"");
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command= "sumatra -t 0.9 -x -n " file " " trnalib() " 2>> /dev/null";
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command= "sumatra -x -n " file " " trnalib() " 2>> /dev/null";
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while ((command | getline output) > 0) {
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split(output,field," ");
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match(field[2],"trn.M?");
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trna=substr(field[2],RSTART,RLENGTH);
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n[trna]+=field[5];
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sub("_"," ",field[2]);
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split(field[2],f2," ");
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trna=f2[1];
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ac=f2[2];
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res[ac][trna]=field[3];
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}
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close(command)
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nmax=0;
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for (i in n) {
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dist=n[i];
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if (n[i] > nmax) {
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nmax=n[i];
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trna=i;
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}
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i=0;
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for (ac in res) {
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max=0;
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for (trna in res[ac]) {
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s=res[ac][trna]
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if (s > max) {
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max=s
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tmax=trna
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}
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}
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i++;
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maxi[tmax][ac]=1
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}
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score["trnfM"]=0;
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score["trnM"]=0;
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score["trnI"]=0;
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for (trna in maxi) {
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score[trna]=length(maxi[trna])
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}
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scores="alternative CAU scores :"
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max=0
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for (trna in score) {
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if (score[trna] > max) {
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max=score[trna];
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tmax=trna;
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}
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scores=scores" "trna"=" score[trna];
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}
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trna=tmax
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}
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else {
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trna="trn" AA1[substr(trna,6,3)];
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scores="-"
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}
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return trna;
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resultat=trna"@"scores
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return resultat;
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}
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function gene2product(gene) {
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return "tRNA-" AA3[substr(gene,4,1)];
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}
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function emblTRNA(geneid,trna,loc,anti,intron,seq) {
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function emblTRNA(geneid,trna,loc,anti,intron,notes,seq) {
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if (loc ~ /^c/) {
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complement=1;
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match(loc,",[0-9][0-9]*\\]");
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@ -132,7 +168,10 @@ function emblTRNA(geneid,trna,loc,anti,intron,seq) {
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print "FT /gene=\""trna"\"";
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print "FT /anticodon=\""anti"\"";
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print "FT /product=\""product"("anti")\"";
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print "FT /inference=\"Aragorn-1.2.38\"";
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if (notes!="-")
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print "FT /note=\""notes"\"";
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}
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function gffTRNA(geneid,trna,loc,anti,intron,seq) {
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@ -239,9 +278,12 @@ BEGIN {
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((geneid != "") && /^[0-9]+/ && ! /genes found/) \
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{ seq=epissage(intron,seq);
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trna=patchtRNA(anti,trna,seq);
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xxx=patchtRNA(anti,trna,seq)
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split(xxx,trnadata,"@");
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# print geneid,trna,loc,anti,"'"intron"'",seq;
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emblTRNA(geneid,trna,loc,anti,intron,seq);
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emblTRNA(geneid,trnadata[1],loc,anti,intron,trnadata[2],seq);
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seq=""
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}
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@ -263,7 +305,10 @@ BEGIN {
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}
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END { seq=epissage(intron,seq);
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trna=patchtRNA(anti,trna,seq);
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xxx=patchtRNA(anti,trna,seq)
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split(xxx,trnadata,"@");
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# print geneid,trna,loc,anti,"'"intron"'",seq;
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emblTRNA(geneid,trna,loc,anti,intron,seq);
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emblTRNA(geneid,trnadata[1],loc,anti,intron,trnadata[2],seq);
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}
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