7 Commits

6 changed files with 117 additions and 105 deletions

5
.gitignore vendored
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@ -1,8 +1,12 @@
/.gitignore
/.cproject
/.project
# /src/
/src/ecoPCR
/src/ecofind
/src/*.P
/src/*.o
/src/ecogrep
# /src/libapat/
@ -14,3 +18,4 @@
# /src/libthermo/
/src/libthermo/*.P

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@ -1 +1 @@
0.8.0
1.0.0

Binary file not shown.

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@ -6,7 +6,7 @@
#include <getopt.h>
#define VERSION "0.8.0"
#define VERSION "1.0.1"
/* ----------------------------------------------- */
@ -80,7 +80,7 @@ static void PrintHelp()
PP "column 21 : sequence\n");
PP "column 22 : definition\n");
PP "------------------------------------------\n");
PP " http://www.grenoble.prabi.fr/trac/ecoPCR/\n");
PP " https://git.metabarcoding.org/obitools/ecopcr/wikis/home\n");
PP "------------------------------------------\n\n");
PP "\n");
@ -566,11 +566,11 @@ int main(int argc, char **argv)
)
{
scname = taxonomy->taxons->taxon[seq->taxid].name;
strncpy(head,seq->SQ,10);
head[10]=0;
strncpy(tail,seq->SQ+seq->SQ_length-10,10);
tail[10]=0;
//scname = taxonomy->taxons->taxon[seq->taxid].name;
//strncpy(head,seq->SQ,10);
//head[10]=0;
//strncpy(tail,seq->SQ+seq->SQ_length-10,10);
//tail[10]=0;
apatseq=ecoseq2apatseq(seq,apatseq,circular);
@ -610,18 +610,21 @@ int main(int argc, char **argv)
{
posj+=o2c->patlen;
// printf("coucou %d %d %d\n",posi,posj,apatseq->seqlen);
errj =apatseq->hiterr[1]->val[j];
errj = apatseq->hiterr[1]->val[j];
length = 0;
if (posj > posi)
length=posj - posi - o1->patlen - o2->patlen;
if (posj < posi)
length= posj + apatseq->seqlen - posi - o1->patlen - o2->patlen;
if (length &&
length = posj - posi - o1->patlen - o2->patlen;
else {
if (circular > 0)
length = posj + apatseq->seqlen - posi - o1->patlen - o2->patlen;
}
if ((length>0) && // For when primers touch or overlap
(!lmin || (length >= lmin)) &&
(!lmax || (length <= lmax)))
(!lmax || (length <= lmax)))
{
printRepeat(seq,oligo1,oligo2,&tparm,o1,o2c,'D',kingdom_mode,posi,posj,erri,errj,taxonomy,delta);
//printf("%s\tD\t%s...%s (%d)\t%d\t%d\t%d\t%d\t%s\n",seq->AC,head,tail,seq->SQ_length,o1Hits,o2cHits,posi,posj,scname);
}
}
}
}
@ -647,7 +650,7 @@ int main(int argc, char **argv)
}
o1cHits = ManberAll(apatseq,o1c,3,begin,length);
// printf("circular= %d\n",circular);
if (o1cHits)
for (i=0; i < o2Hits;i++)
{
@ -666,15 +669,19 @@ int main(int argc, char **argv)
length = 0;
if (posj > posi)
length=posj - posi + 1 - o2->patlen - o1->patlen; /* - o1->patlen : suppress by <EC> */
if (posj < posi)
length= posj + apatseq->seqlen - posi - o1->patlen - o2->patlen;
length = posj - posi + 1 - o2->patlen - o1->patlen; /* - o1->patlen : deleted by <EC> (prior to the OBITools3) */
else {
if (circular > 0)
length = posj + apatseq->seqlen - posi - o1->patlen - o2->patlen;
}
if (length &&
if ((length>0) && // For when primers touch or overlap
(!lmin || (length >= lmin)) &&
(!lmax || (length <= lmax)))
(!lmax || (length <= lmax)))
{
printRepeat(seq,oligo1,oligo2,&tparm,o2,o1c,'R',kingdom_mode,posi,posj,erri,errj,taxonomy,delta);
//printf("%s\tR\t%s...%s (%d)\t%d\t%d\t%d\t%d\t%s\n",seq->AC,head,tail,seq->SQ_length,o2Hits,o1cHits,posi,posj,scname);
//printf("%s\tR\t%s...%s (%d)\t%d\t%d\t%d\t%d\t%s\n",seq->AC,head,tail,seq->SQ_length,o2Hits,o1cHits,posi,posj,scname);
}
}
}
}

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@ -12,14 +12,14 @@ ecorankidx_t *read_rankidx(const char *filename)
int32_t i;
int32_t rs;
char *buffer;
f = open_ecorecorddb(filename,&count,1);
index = (ecorankidx_t*) ECOMALLOC(sizeof(ecorankidx_t) + sizeof(char*) * (count-1),
"Allocate rank index");
index->count=count;
index->count=count;
for (i=0; i < count; i++)
{
buffer = read_ecorecord(f,&rs);
@ -27,21 +27,18 @@ ecorankidx_t *read_rankidx(const char *filename)
"Allocate rank label");
strncpy(index->label[i],buffer,rs);
}
return index;
}
int32_t rank_index(const char* label,ecorankidx_t* ranks)
{
char **rep;
fprintf(stderr,"Looking for rank -%s-... ",label);
rep = bsearch(label,ranks->label,ranks->count,sizeof(char*),compareRankLabel);
if (rep)
return rep-ranks->label;
else
ECOERROR(ECO_NOTFOUND_ERROR,"Rank label not found");
return -1;
}

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@ -5,10 +5,10 @@
static ecotx_t *readnext_ecotaxon(FILE *f,ecotx_t *taxon);
/**
* Open the taxonomy database
* @param pointer to the database (.tdx file)
* @return a ecotxidx_t structure
/**
* Open the taxonomy database
* @param pointer to the database (.tdx file)
* @return a ecotxidx_t structure
*/
ecotxidx_t *read_taxonomyidx(const char *filename,const char *filename2)
{
@ -18,22 +18,22 @@ ecotxidx_t *read_taxonomyidx(const char *filename,const char *filename2)
FILE *f2;
ecotxidx_t *index;
int32_t i;
f = open_ecorecorddb(filename,&count,1);
f2 = open_ecorecorddb(filename2,&count2,0);
index = (ecotxidx_t*) ECOMALLOC(sizeof(ecotxidx_t) + sizeof(ecotx_t) * (count+count2-1),
"Allocate taxonomy");
index->count=count+count2;
fprintf(stderr,"Reading %d taxa...\n",count);
for (i=0; i < count; i++){
readnext_ecotaxon(f,&(index->taxon[i]));
index->taxon[i].parent=index->taxon + (int32_t)index->taxon[i].parent;
}
}
if (count2>0)
fprintf(stderr,"Reading %d local taxa...\n",count2);
@ -52,18 +52,18 @@ ecotxidx_t *read_taxonomyidx(const char *filename,const char *filename2)
int32_t delete_taxonomy(ecotxidx_t *index)
{
int32_t i;
if (index)
{
for (i=0; i< index->count; i++)
if (index->taxon[i].name)
ECOFREE(index->taxon[i].name,"Free scientific name");
ECOFREE(index,"Free Taxonomy");
return 0;
}
return 1;
}
@ -75,32 +75,32 @@ int32_t delete_taxon(ecotx_t *taxon)
{
if (taxon->name)
ECOFREE(taxon->name,"Free scientific name");
ECOFREE(taxon,"Free Taxon");
return 0;
}
return 1;
}
/**
* Read the database for a given taxon a save the data
* Read the database for a given taxon a save the data
* into the taxon structure(if any found)
* @param *f pointer to FILE type returned by fopen
* @param *taxon pointer to the structure
*
* @return a ecotx_t structure if any taxon found else NULL
*
* @return a ecotx_t structure if any taxon found else NULL
*/
ecotx_t *readnext_ecotaxon(FILE *f,ecotx_t *taxon)
{
ecotxformat_t *raw;
int32_t rs;
raw = read_ecorecord(f,&rs);
if (!raw)
return NULL;
@ -109,18 +109,18 @@ ecotx_t *readnext_ecotaxon(FILE *f,ecotx_t *taxon)
raw->namelength = swap_int32_t(raw->namelength);
raw->parent = swap_int32_t(raw->parent);
raw->rank = swap_int32_t(raw->rank);
raw->taxid = swap_int32_t(raw->taxid);
raw->taxid = swap_int32_t(raw->taxid);
}
taxon->parent = (ecotx_t*)raw->parent;
taxon->taxid = raw->taxid;
taxon->rank = raw->rank;
taxon->name = ECOMALLOC((raw->namelength+1) * sizeof(char),
"Allocate taxon scientific name");
strncpy(taxon->name,raw->name,raw->namelength);
return taxon;
}
@ -131,26 +131,26 @@ ecotaxonomy_t *read_taxonomy(const char *prefix,int32_t readAlternativeName)
char *filename;
char *filename2;
int buffsize;
tax = ECOMALLOC(sizeof(ecotaxonomy_t),
"Allocate taxonomy structure");
buffsize = strlen(prefix)+10;
filename = ECOMALLOC(buffsize,
"Allocate filename");
filename2= ECOMALLOC(buffsize,
"Allocate filename");
snprintf(filename,buffsize,"%s.rdx",prefix);
tax->ranks = read_rankidx(filename);
snprintf(filename,buffsize,"%s.tdx",prefix);
snprintf(filename2,buffsize,"%s.ldx",prefix);
tax->taxons = read_taxonomyidx(filename,filename2);
if (readAlternativeName)
{
snprintf(filename,buffsize,"%s.ndx",prefix);
@ -159,7 +159,7 @@ ecotaxonomy_t *read_taxonomy(const char *prefix,int32_t readAlternativeName)
else
tax->names=NULL;
return tax;
}
@ -170,15 +170,15 @@ int32_t delete_ecotaxonomy(ecotaxonomy_t *taxonomy)
{
if (taxonomy->ranks)
ECOFREE(taxonomy->ranks,"Free rank index");
if (taxonomy->taxons)
ECOFREE(taxonomy->taxons,"Free taxon index");
ECOFREE(taxonomy,"Free taxonomy structure");
return 0;
}
return 1;
}
@ -187,17 +187,17 @@ ecotx_t *eco_findtaxonatrank(ecotx_t *taxon,
{
ecotx_t *current_taxon;
ecotx_t *next_taxon;
current_taxon = taxon;
next_taxon = current_taxon->parent;
while ((current_taxon!=next_taxon) && // I' am the root node
(current_taxon->rank!=rankidx))
{
current_taxon = next_taxon;
next_taxon = current_taxon->parent;
}
if (current_taxon->rank==rankidx)
return current_taxon;
else
@ -207,19 +207,19 @@ ecotx_t *eco_findtaxonatrank(ecotx_t *taxon,
/**
* Get back information concerning a taxon from a taxonomic id
* @param *taxonomy the taxonomy database
* @param taxid the taxonomic id
*
* @result a ecotx_t structure containing the taxonimic information
* @param taxid the taxonomic id
*
* @result a ecotx_t structure containing the taxonimic information
**/
ecotx_t *eco_findtaxonbytaxid(ecotaxonomy_t *taxonomy,
ecotx_t *eco_findtaxonbytaxid(ecotaxonomy_t *taxonomy,
int32_t taxid)
{
ecotx_t *current_taxon;
int32_t taxoncount;
int32_t i;
taxoncount=taxonomy->taxons->count;
for (current_taxon=taxonomy->taxons->taxon,
i=0;
i < taxoncount;
@ -229,30 +229,30 @@ ecotx_t *eco_findtaxonbytaxid(ecotaxonomy_t *taxonomy,
return current_taxon;
}
}
return (ecotx_t*)NULL;
return (ecotx_t*)NULL;
}
/**
* Find out if taxon is son of other taxon (identified by its taxid)
* @param *taxon son taxon
* @param parent_taxid taxonomic id of the other taxon
*
*
* @return 1 is the other taxid math a parent taxid, else 0
**/
int eco_isundertaxon(ecotx_t *taxon,
int eco_isundertaxon(ecotx_t *taxon,
int other_taxid)
{
ecotx_t *next_parent;
next_parent = taxon->parent;
while ( (other_taxid != next_parent->taxid) &&
next_parent = taxon->parent;
while ( (other_taxid != next_parent->taxid) &&
(strcmp(next_parent->name, "root")) )
{
next_parent = next_parent->parent;
}
if (other_taxid == next_parent->taxid)
return 1;
else
@ -264,16 +264,16 @@ ecotx_t *eco_getspecies(ecotx_t *taxon,
{
static ecotaxonomy_t *tax=NULL;
static int32_t rankindex=-1;
if (taxonomy && tax!=taxonomy)
{
rankindex = rank_index("species",taxonomy->ranks);
tax=taxonomy;
}
if (!tax || rankindex < 0)
ECOERROR(ECO_ASSERT_ERROR,"No taxonomy defined");
return eco_findtaxonatrank(taxon,rankindex);
}
@ -282,16 +282,16 @@ ecotx_t *eco_getgenus(ecotx_t *taxon,
{
static ecotaxonomy_t *tax=NULL;
static int32_t rankindex=-1;
if (taxonomy && tax!=taxonomy)
{
rankindex = rank_index("genus",taxonomy->ranks);
tax=taxonomy;
}
if (!tax || rankindex < 0)
ECOERROR(ECO_ASSERT_ERROR,"No taxonomy defined");
return eco_findtaxonatrank(taxon,rankindex);
}
@ -301,16 +301,16 @@ ecotx_t *eco_getfamily(ecotx_t *taxon,
{
static ecotaxonomy_t *tax=NULL;
static int32_t rankindex=-1;
if (taxonomy && tax!=taxonomy)
{
rankindex = rank_index("family",taxonomy->ranks);
tax=taxonomy;
}
if (!tax || rankindex < 0)
ECOERROR(ECO_ASSERT_ERROR,"No taxonomy defined");
return eco_findtaxonatrank(taxon,rankindex);
}
@ -319,16 +319,16 @@ ecotx_t *eco_getkingdom(ecotx_t *taxon,
{
static ecotaxonomy_t *tax=NULL;
static int32_t rankindex=-1;
if (taxonomy && tax!=taxonomy)
{
rankindex = rank_index("kingdom",taxonomy->ranks);
tax=taxonomy;
}
if (!tax || rankindex < 0)
ECOERROR(ECO_ASSERT_ERROR,"No taxonomy defined");
return eco_findtaxonatrank(taxon,rankindex);
}
@ -337,15 +337,18 @@ ecotx_t *eco_getsuperkingdom(ecotx_t *taxon,
{
static ecotaxonomy_t *tax=NULL;
static int32_t rankindex=-1;
if (taxonomy && tax!=taxonomy)
{
rankindex = rank_index("superkingdom",taxonomy->ranks);
if (rankindex < 0) {
rankindex = rank_index("domain",taxonomy->ranks);
}
tax=taxonomy;
}
if (!tax || rankindex < 0)
ECOERROR(ECO_ASSERT_ERROR,"No taxonomy defined");
return eco_findtaxonatrank(taxon,rankindex);
}